Genital tract sample collection tube device
By using zirconia beads and sample lysis buffer in the reproductive tract sample collection tube, efficient collection, transportation, and preservation of reproductive tract samples were achieved, solving the problems of long sample processing cycles and contamination risks, and improving the accuracy and efficiency of testing.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2026-03-24
AI Technical Summary
In existing technologies, the collection, storage and transportation of reproductive tract samples suffer from long sample processing cycles, insufficient timeliness and accuracy of testing, and the risk of contamination during the sampling process.
Design a reproductive tract sample collection tube device, comprising zirconium oxide beads and sample lysis buffer, which uses mechanical friction to break down the cell walls of pathogens and release nucleic acids, achieving fully enclosed sample transport and avoiding contamination.
It improves the accuracy and efficiency of sample testing, reduces sample processing time, lowers the risk of contamination, and ensures the integrity and preservation performance of samples during transportation.
Smart Images
Figure CN224023589U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to medical equipment technical field, concretely is a genital tract sample collection tube device. BACKGROUND
[0002] The storage, transportation and extraction efficiency of the sample of special pathogenic bacteria can affect the accuracy of detection, so the pretreatment step before extraction is very important. At present, the collected sample is usually stored in a conventional storage tube, and needs to be ground by a homogenizer after entering the laboratory. This operation can cause the sample processing period to be lengthened, thereby affecting the timeliness and accuracy of sample detection.
[0003] In addition, the clinical genital tract microbial sampling usually uses a disposable medical cotton swab, and after sampling, the cotton swab is placed into a disposable open plastic test tube and then the sampling specimen is transported. The disposable cotton swab and the test tube are not independently sealed and packaged. Since the patient is manually transported, there is a risk of dropping or misplacing. Whether before sampling or during transportation, the sampling specimen cannot be guaranteed to be contaminated. If the sampling specimen is contaminated, the detection result can be affected and the doctor's diagnosis can be disturbed. Therefore, a device for assisting sample collection, transportation and storage is needed to improve the accuracy of the detection result. SUMMARY
[0004] The utility model discloses a genital tract sample collection tube device to solve the problem in the above background art. The device not only helps to store the sample, but also improves the experimental efficiency. After the sample is collected, it is placed in the test tube containing the sample lysate provided by the utility model, which can continuously lyse the pathogenic bacteria and release nucleic acids. In addition, the collection tube is provided with zirconium oxide beads for grinding, which can provide continuous mechanical friction during sample transportation, thereby improving the lysis effect of special pathogenic bacteria. After sampling, the sample is directly sealed and transported, avoiding the risk of dropping, misplacing and sample contamination.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:
[0006] The utility model provides a genital tract sample collection tube device, including the pipe body, the pipe cover, the sampling cotton swab, the pipe body is provided with zirconium oxide bead in, the pipe body top is provided with the flared section, the flared section outside is provided with the outer thread, the inner wall of flared section and the pipe body inner wall are connected through the inclined plane, the sampling cotton swab is supported and set up on the inclined plane, the pipe cover is provided with the cavity in, the pipe cover bottom inner wall is provided with the inner thread, and the inner thread is matched with the outer thread and is set up, the cavity is provided with the sample lysate, and the cavity is provided with the lancet, and the lancet top penetrates the pipe cover top and is fixedly connected to the lancet push plate, and the C type stopper is clamped and set up between the lancet push plate and the pipe cover.
[0007] Further, the sampling cotton swab comprises a pushing barrel, the top end of the pushing barrel is provided with a limiting plate, the limiting plate is arranged on an inclined surface, a pushing rod is arranged in the pushing barrel, a cotton swab rod is arranged at the bottom of the pushing barrel, a plurality of limiting rings are arranged at the top of the cotton swab rod, the limiting rings are arranged in the pushing barrel, and a cotton head is arranged at the bottom of the cotton swab rod; the cotton swab rod is pushed by the pushing rod, the cotton swab rod is separated from the pushing barrel, the cotton head is arranged in the pipe body, contact is reduced, pollution is avoided, and the sealing of the pipe cover of the pipe body is facilitated.
[0008] Further, the pushing barrel is in an inverted conical shape; the patient is prevented from being scratched by edges and corners, the part in contact with the cotton swab rod is relatively thin, and the cotton swab rod is facilitated to be pushed out.
[0009] Further, the top of the pushing rod is a pushing plate, and the cotton swab rod is facilitated to be pushed out.
[0010] Further, the lancet and the cover are connected through threads, and the cavity is sealed.
[0011] Further, the cavity has a cylindrical cavity structure formed by a vertically extending side wall.
[0012] Further, the bottom of the cavity is a film, and the film is facilitated to be pierced.
[0013] Further, the zirconium oxide beads have a diameter of 0.3-5 mm and are arranged at the bottom of the pipe body of the sampling tube; the sampling tube is used in cooperation with a small amount of sterile zirconium oxide beads, mechanical friction is generated in the sample transportation process, laboratory operation is more convenient, time and labor are saved, and the like.
[0014] Further, the pipe body of the sampling tube has a length of 95-110 mm and is made of transparent sterile medical-grade material, the state of the sample is facilitated to be observed, and the chemical stability of the sample is ensured.
[0015] The pipe cover has a diameter of 12-18 mm, is made of PV material plastic, is connected to the top of the pipe body of the sampling tube, is good in sealing performance, is easy to operate, and can avoid that the sample is polluted by the outside.
[0016] The sampling tube is sealed through thread design, and the tube is facilitated to be inverted to mix substances.
[0017] The sample lysis liquid has a volume of 1-5 mL, the lysis liquid contains a specific formula capable of inhibiting microbial growth and lysis and releasing sample nucleic acid, and the sample storage time can be effectively prolonged.
[0018] Compared with the prior art, the utility model has the beneficial effects that:
[0019] The utility model is used in cooperation with the sample lysis liquid and the zirconium oxide beads in the sampling tube, the storage performance of the collected sample is ensured, downstream experiment requirements can be met, and the qualified rate of the sample is improved.
[0020] The collecting tube device provided by the utility model spares the experiment platform from using a homogenizer to process the sample twice, reduces the sample processing time, and improves the experiment efficiency.
[0021] The reproductive tract sample collecting tube device provides a safe and efficient solution for sample collection, transportation and long-term storage.
[0022] After sampling, the sample is pushed into the tube body without contact, and then the tube cover is sealed, so that the sample is directly sealed and conveyed after sampling, and the risk of falling, misplacing and sample contamination is avoided.
[0023] The utility model realizes the pre-storage of the lysis liquid, realizes the integrated collection and pretreatment of the sample by adding the lysis liquid in the fully closed state of the sample, is simple and convenient to operate, not only saves the operation time of the experiment personnel and reduces the labor intensity, but also greatly improves the detection efficiency. BRIEF DESCRIPTION OF DRAWINGS
[0024] Figure 1 It is a three-dimensional structure schematic view of the reproductive tract sample collecting tube device of the utility model
[0025] Figure 2 It is an internal structure schematic view of the reproductive tract sample collecting tube device of the utility model
[0026] Figure 3 It is the utility model Figure 2 It is an enlarged view of the middle part A.
[0027] BRIEF DESCRIPTION OF DRAWINGS
[0028] 1, tube cover, 2, tube body, 3, needle push plate, 4, C type limiting piece, 5, flared section, 6, zirconia bead, 7, cotton head, 8, cotton swab rod, 9, push cylinder, 10, push rod, 11, limiting plate, 12, push plate, 13, needle, 14, cavity, 15, film, 16, limiting ring. DETAILED DESCRIPTION
[0029] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model. Embodiment 1
[0030] As Figures 1-3As shown, a reproductive tract sample collection tube device includes a tube body, a tube cover, a sampling cotton swab, a zirconium oxide bead is arranged in the tube body, a flared section is arranged at the top of the tube body, an external thread is arranged outside the flared section, the inner wall of the flared section is connected with the inner wall of the tube body through a slope, the sampling cotton swab is supported and arranged on the slope, a cavity is arranged in the tube cover, an internal thread is arranged on the inner wall of the bottom of the tube cover, the internal thread is arranged in matching with the external thread, a sample lysate is arranged in the cavity, a lancet is arranged in the cavity, the top of the lancet penetrates through the top of the tube cover and is fixedly connected with a lancet push plate, a C-shaped limiting piece is clamped between the lancet push plate and the tube cover.
[0031] The sampling cotton swab includes a push barrel, a limiting plate is arranged at the top end of the push barrel, the limiting plate is arranged on the slope, a push rod is arranged in the push barrel, a cotton swab rod is arranged at the bottom of the push barrel, a plurality of limiting rings are arranged at the top of the cotton swab rod, the limiting rings are arranged in the push barrel, and a cotton head is arranged at the bottom of the cotton swab rod; the cotton swab rod is pushed by the push rod to be separated from the push barrel, the cotton head is arranged in the tube body, contact is reduced, pollution is avoided, and sealing of the tube body and the tube cover is facilitated. The push barrel is in an inverted conical shape; edge scratches of a patient are avoided, the part in contact with the cotton swab rod is relatively thin, and the cotton swab rod is facilitated to be pushed out. The top of the push rod is a push plate, and the cotton swab rod is facilitated to be pushed out by force.
[0032] The lancet is connected with the tube cover through a thread.
[0033] The cavity has a cylindrical cavity structure formed by a vertically extending side wall; the bottom of the cavity is a film.
[0034] The length of the tube body of the collection tube is 95-110 mm, the tube body is made of transparent sterile medical-grade material, the state of the sample is facilitated to be observed, and the chemical stability of the sample is ensured.
[0035] The diameter of the tube cover is 12-18 mm, the tube cover is made of PV material plastic connected with the top of the tube body of the collection tube, is good in sealing performance, easy to operate, and can avoid pollution of the sample by the outside world.
[0036] Preferably, a thread is arranged on the tube cover, the tube cover is connected with the tube body through the thread design, the collection tube is sealed, and the test tube mixture is facilitated to be mixed upside down.
[0037] The volume of the sample lysate is 1-5 mL, the lysate contains a specific formula capable of inhibiting microbial growth and lysing and releasing sample nucleic acids, and can effectively prolong the sample storage time.
[0038] The diameter of the zirconium oxide bead is 0.3-5 mm, the zirconium oxide bead is a white substance at the bottom of the collection tube, a small amount of sterile zirconium oxide bead is used in cooperation with the sample collection tube to generate mechanical friction during sample transportation, so that laboratory operation is more convenient, time-saving and labor-saving.
[0039] In the embodiment of the utility model, the tube body length of sample collection tube is 98mm cylindrical, the tube cover diameter is 18mm, the required sample lysate volume is 3mL, the zirconium oxide bead diameter used is 1mm.
[0040] In the embodiment of the utility model, the sample lysate contains a specific formula, specifically SDS, TritonX-100, EDTA, Proclin300Plus.
[0041] Working principle: when sampling, unscrew the tube cover, take out the sampling cotton swab after sampling, hold the limiting plate with fingers, push the push plate, push the cotton swab rod out of the push cylinder, place it in the tube body, screw on the tube cover, remove the C-shaped limiting piece, then rotate the needle push plate to separate the needle from the tube cover and push down at the same time, pierce the film, and the sample lysate flows into the tube body. Embodiment 2
[0042] Performance comparison of collection tube
[0043] S1-1: sample collection. Two samples are collected from the same part of the subject using a collection cotton swab, and then the two samples are placed in a collection tube containing sample lysate and zirconium oxide beads and a collection tube containing 95% ethanol. After screwing the tube cover, the two test tubes should be inverted to mix the contents. After completing the above steps, the two collection tubes are properly packaged and sent to the laboratory for subsequent experiments.
[0044] S1-2: sample transportation. During sample transportation, the zirconium oxide beads in the collection tube containing zirconium oxide beads and sample lysate will continuously generate mechanical friction due to jolting, which helps to destroy the cell walls of certain pathogenic bacteria. At the same time, the use of sample lysate with the collection tube can effectively destroy pathogenic bacteria and release nucleic acids, ensuring the accuracy of the test results.
[0045] S1-3: sample experiment. After receiving the sample, two collection tubes described in S1-1 are used for experiments. The collection tube containing 95% ethanol needs to be centrifuged at high speed to obtain the precipitate in the tube, and then the same volume of sample lysate and the same volume of zirconium oxide beads are added, and then the homogenizer is used for grinding and extraction; while using the collection tube of the utility model, the sample is directly extracted after being stored at room temperature for one day, three days, five days and seven days.
[0046] S1-4: comparison of experimental results. The extraction results of the collection tube containing sample lysate and zirconium oxide beads and the collection tube containing 95% ethanol are compared, and it is found that there is no significant difference, see Table 1.
[0047] Table 1. Comparison of experimental results of different collection tubes
[0048] It can be known from Table 1 that the samples collected in the collecting tube provided by the utility model are respectively stored for one day, three days, five days and seven days, and then the 16S CT value, RNase P CT value and sample concentration of the samples are compared with the related values of the samples stored in the collecting tube containing 95% ethanol. In the aspect of 16S CT value, the sample stored in the collecting tube provided by the utility model and the control sample have the largest difference on the third day, and the difference value is 0.10. In the aspect of RNase P CT value, the sample stored in the collecting tube provided by the utility model and the control sample have the largest difference on the third day, and the difference value is 0.33. In the aspect of concentration, the sample stored in the collecting tube provided by the utility model and the control sample have the largest difference on the seventh day, and the difference value is 3.15. The above difference values all have no significant difference, that is, the collecting tube provided by the utility model has high accuracy and good storage effect.
[0049] It should be noted that, in this document, the terms such as first and second are used merely to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that these entities or operations have any such actual relationship or order. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed or inherent to such a process, method, article or device.
[0050] It should be noted that the above only illustrates the technical idea of the utility model, and cannot limit the protection scope of the utility model. For ordinary skilled persons in the art, without departing from the principle of the utility model, a number of improvements and refinements can be made, which all fall within the protection scope of the utility model claim.
Claims
1. A reproductive tract sample collection tube device, comprising: The sampling cotton swab comprises a tube body, a tube cover, and a sampling cotton swab, the tube body is internally provided with a zirconium oxide bead, the top of the tube body is provided with an expanded section, the expanded section is externally provided with an external thread, the inner wall of the expanded section is connected with the inner wall of the tube body through a slope, the sampling cotton swab is supported and arranged on the slope, the tube cover is internally provided with a cavity, the bottom inner wall of the tube cover is provided with an internal thread, the internal thread is arranged in matching with the external thread, the cavity is internally provided with a sample lysate, the cavity is internally provided with a lancet, the top of the lancet penetrates through the top of the tube cover and is fixedly connected with a lancet push plate, and a C-shaped limiting piece is clamped between the lancet push plate and the tube cover.
2. A reproductive tract specimen collection tube device according to claim 1, wherein, The sampling cotton swab comprises a push cylinder, the top end of the push cylinder is provided with a limiting plate, the limiting plate is arranged on the slope, the push cylinder is internally provided with a push rod, the bottom of the push cylinder is provided with a cotton swab rod, the top of the cotton swab rod is provided with a plurality of limiting rings, the limiting rings are arranged in the push cylinder, and the bottom of the cotton swab rod is provided with a cotton head.
3. A reproductive tract specimen collection tube device according to claim 2, wherein, The push cylinder is in an inverted conical shape.
4. The reproductive tract specimen collection tube device of claim 2, wherein, The top of the push rod is a push plate.
5. The reproductive tract specimen collection tube device of claim 2, wherein, The lancet is connected with the tube cover through a thread.
6. The reproductive tract specimen collection tube device of claim 1, wherein, The cavity has a cylindrical cavity structure formed by a vertically extending side wall.
7. The device of claim 1, wherein the device is a female reproductive tract sample collection tube device. The bottom of the cavity is a film.
8. The reproductive tract specimen collection tube device of claim 1, wherein, The zirconium oxide bead has a diameter of 0.3-5 mm and is placed at the bottom of the tube body of the collection tube.