Centrifugal tube capable of extracting supernatant or turbid liquid

Abstract

The utility model discloses a centrifuge tube capable of extracting supernate or turbid liquid, which relates to the technical field of centrifugal separation devices, and comprises a test tube, an inner sleeve, at least two one-way valves, a test tube cover and a screw cap, the inner sleeve is arranged in the test tube, the bottom of the inner sleeve is provided with the one-way valves, the one-way valves are opposite in direction, the test tube cover covers the test tube, and the screw cap covers the test tube. The screw cap penetrates through the test tube cover and is in shaft connection with the test tube cover, a screw rod is arranged at one end, located in the test tube cover, of the screw cap, the screw rod is in threaded connection with the open end of the inner sleeve, and a closable pressure relief hole is formed in the test tube cover. A user can adjust the bottom of the inner sleeve to the junction of a turbid liquid and a supernatant according to previous experience, the turbid liquid is located in the test tube and the supernatant is located in the inner sleeve after centrifugation, the centrifugal separation function is achieved, the operation steps of centrifugal separation and extraction are simplified, the sampling and collecting speed is increased, and the application range is wider.

Description

TECHNICAL FIELD

[0001] The utility model relates to centrifugal separation device technical field especially, it relates to a centrifugal tube that can extract supernatant or turbidity liquid. BACKGROUND

[0002] Whether to take supernatant or discard supernatant after centrifugation depends on experimental objectives and requirements.

[0003] 1. Take supernatant after centrifugation: This approach is to obtain supernatant after centrifugation, which may contain the desired target substance. For example, in the experiment of extracting and purifying metagenomic DNA, the supernatant needs to be obtained to remove impurities and unwanted substances through this step.

[0004] 2. Discard supernatant after centrifugation: This approach is to remove the supernatant, which may contain unwanted substances. For example, in cell culture experiments, the supernatant may need to be discarded after centrifugation to remove nutrients and cell debris in the culture medium.

[0005] Different experiments have different objectives and requirements, and the decision to take supernatant or discard supernatant after centrifugation needs to be made according to the specific experimental steps and requirements to ensure proper operation of the centrifuge and obtain the desired experimental results.

[0006] Patent document (CN213245011U) provides a double-cavity centrifugal tube integrating suspension separation and supernatant extraction functions, which sets a partition in the test tube and sets a elastic one-way valve on the partition, longitudinally dividing the test tube cavity into a sample cavity and a supernatant collection cavity. The elastic one-way valve is provided with a separation hole, a baffle, a mass block and an elastic structure. By inserting the double-cavity centrifugal tube into the fixed angle rotor of the centrifuge, the liquid zone inside the centrifugal tube can be divided by changing the positions of the sample cavity and the supernatant collection cavity. This patent uses centrifugal force as the switch of the one-way valve to realize the separation and extraction of the supernatant. This method is extremely efficient, fast and pure when applied to situations where supernatant needs to be extracted.

[0007] The above disclosure file separates and extracts the supernatant by opening the one-way valve switch through the action of centrifugation, but also has the following shortcomings:

[0008] (1) The one-way valve is opened by the action of centrifugal force, allowing the supernatant to enter the collection cavity, but only part of the supernatant enters the collection cavity during actual operation, and it cannot be completely separated.

[0009] (2) This invention can only extract supernatant, and the extraction of turbidity liquid still needs manual operation.

[0010] Therefore, a centrifugal tube that can extract supernatant or turbidity liquid is proposed. UTILITY MODEL CONTENTS

[0011] The centrifugal tube capable of extracting supernatant or turbid liquid is provided, and at least one problem in the above background art is solved.

[0012] To achieve the above object, the utility model provides the following technical scheme:

[0013] A centrifugal tube capable of extracting supernatant or turbid liquid, including test tube, inner sleeve, check valve, test tube cover and screw cap, the inner sleeve is arranged in the test tube, the bottom of the inner sleeve is provided with at least two check valves in opposite directions, the check valve includes valve and valve sheet, the test tube cover is covered on the test tube, the screw cap passes through the test tube cover, and the test tube cover forms the shaft joint relationship, one end of the screw cap located outside the test tube cover is provided with a knob, one end of the screw cap located inside the test tube cover is provided with a screw rod, the screw rod and the opening end of the inner sleeve form the threaded connection relationship, and the test tube cover is provided with a closable pressure relief hole.

[0014] Further, the test tube side is provided with a scale line.

[0015] Further, the valve sheet on the check valve is made of elastic material.

[0016] Further, the valve sheet on the check valve is made of corrosion-resistant rubber material.

[0017] Further, the pressure relief hole is closed by a small cover that can be screwed on the small hole.

[0018] Further, the knob on the screw cap is provided with an anti-slip pattern.

[0019] Compared with the prior art, the utility model has the following beneficial effects:

[0020] 1, the utility model discloses a check valve arranged at the bottom of the inner sleeve, which divides the test tube into a turbid liquid area and the inner sleeve into a supernatant area, so that the user can adjust the bottom of the inner sleeve to the junction of the turbid liquid and the supernatant according to previous experience, and finally, after centrifugation, the turbid liquid is located in the test tube, and the supernatant is located in the inner sleeve, realizing the centrifugal separation function, simplifying the operation steps of centrifugal separation extraction and accelerating the speed of sampling collection.

[0021] 2, the utility model can extract supernatant or turbid liquid according to experimental requirements, and has a wider application range. DRAWINGS

[0022] Figure 1 It is an explosion schematic view of the centrifugal tube capable of extracting supernatant or turbid liquid.

[0023] Figure 2 It is a sectional view of the centrifugal tube capable of extracting supernatant or turbid liquid.

[0024] Figure 3 A one-way valve closing schematic view of a centrifugal tube capable of extracting supernatant or precipitate;

[0025] Figure 4 A one-way valve opening schematic view of a centrifugal tube capable of extracting supernatant or precipitate.

[0026] The reference signs in the drawings of the specification include:

[0027] 1, test tube; 2, inner sleeve; 3, one-way valve; 301, valve; 302, valve plate; 4, test tube cover; 5, screw cover; 501, knob; 502, screw rod; 6, pressure relief hole; 7, small cover; 8, scale, 9, anti-skid pattern. DETAILED DESCRIPTION

[0028] The utility model will be further described in detail in combination with the drawings and embodiments:

[0029] As Figures 1-4 shown, a centrifugal tube capable of extracting supernatant or precipitate, comprising test tube 1, inner sleeve 2, one-way valve 3, test tube cover 4 and screw cover 5, test tube 1 is provided with inner sleeve 2, test tube 1 side is provided with scale 8, can judge the bottom of inner sleeve 2 is located where according to scale 8, the bottom of inner sleeve 2 is provided with one-way valve 3, one-way valve 3 includes valve 301 and valve plate 302, one-way valve 3 is preferably a plurality of arrays in the bottom of inner sleeve 2, and at least two opposite directions one-way valve 3, when the number of one-way valve 3 is more, the number of one-way valve 3 opening to inner sleeve 2 direction is far less than one-way valve 3 opening to test tube 1 direction, the valve plate 302 on one-way valve 3 is made of elastic material, can be elastically deformed to open valve 301 under the action of centrifugal force, preferably, the valve plate 302 on one-way valve 3 is made of corrosion-resistant rubber material.

[0030] Test tube cover 4 is covered on test tube 1, screw cover 5 passes through test tube cover 4, and test tube cover 4 forms shaft joint relationship, screw cover 5 is provided with knob 501 at one end outside test tube cover 4, knob 501 is provided with anti-skid pattern 9, screw cover 5 is provided with screw rod 502 at one end inside test tube cover 4, and screw rod 502 and the opening end of inner sleeve 2 form threaded connection relationship, because screw cover 5 is provided with screw rod 502 at one end inside test tube cover 4, when rotating knob 501 provided at one end outside test tube cover 4, inner sleeve 2 can move up and down to the appropriate position in test tube 1 under the action of screw thread.

[0031] Test tube cover 4 is provided with closable pressure relief hole 6, and the pressure relief hole 6 is closed by small cover 7 that can be screwed on the small hole, when closing pressure relief hole 6, test tube 1 and test tube cover 4 are closed to form a sealed space, which is convenient for centrifugation, when opening pressure relief hole 6, test tube 1 and inner sleeve 2 can be separated after centrifugation is completed.

[0032] The specific implementation process is as follows:

[0033] First, the centrifugal suspension to be placed in the inner sleeve 2, screw 502 is screwed to the opening of the inner sleeve 2, because the one-way valve 3 at the bottom of the inner sleeve 2 opens to the test tube 1 direction must be opened under the action of a certain threshold force, so the suspension will not leak out of the inner sleeve 2, then the inner sleeve 2 is placed in the test tube 1, and the test tube cover 4 is closed, and then the inner sleeve 2 is moved up and down in the test tube 1 by rotating the knob 501, the experimenter preliminarily confirms the position of the bottom of the inner sleeve 2 at the interface between the supernatant and the turbid liquid according to the previous experience and the scale line 8 on the outer wall of the test tube 1, if the supernatant is needed, the bottom of the inner sleeve 2 is slightly above the interface between the expected turbid liquid and the supernatant, if the supernatant is discarded, the bottom of the inner sleeve 2 is slightly below the interface between the expected turbid liquid and the supernatant, then the pressure relief hole 6 is closed, so as to be sealed, and finally put into the centrifuge, when the centrifugal revolution reaches a certain threshold, the one-way valve 3 at the bottom of the inner sleeve 2 opens to the test tube 1 direction, at this time the inner sleeve 2 is connected with the test tube 1, the suspension enters the test tube 1, because the position of the bottom of the inner sleeve 2 is set at the interface between the turbid liquid and the supernatant, when the centrifugation finally stops, the one-way valve 3 is closed, the turbid liquid is in the test tube 1, and the supernatant is in the inner sleeve 2, the experimenter can extract the supernatant or the turbid liquid as needed, it is worth noting that if the interface between the final turbid liquid and the supernatant is too different from the expected one, the bottom of the inner sleeve 2 can be adjusted to the interface between the final turbid liquid and the supernatant, because the suspension fills the test tube 1 and the inner sleeve 2 at this time, the one-way valve 3 at the bottom of the inner sleeve 2 in the opposite direction can make the inner sleeve 2 move freely forward and backward, after re-centrifugation, the expected material can be obtained to the maximum extent, and waste is reduced, finally, the pressure relief hole 6 on the test tube cover 4 is opened, the inner sleeve 2 in the test tube 1 is taken out, and the supernatant or the turbid liquid is obtained.

[0034] The above is only an embodiment of the present application, and the specific technical solutions and / or common knowledge of the scheme are not described in detail. It should be noted that for those skilled in the art, without departing from the technical scheme of the present application, a number of modifications and improvements can be made, which should also be considered as the protection scope of the present application, which will not affect the effect and practicality of the present application. The protection scope of the present application should be subject to the content of its claims, and the specific implementation mode and the like in the specification can be used to explain the content of the claims.

Claims

1. A centrifuge tube from which supernatant or pellet can be extracted, characterized in that: The utility model relates to a test tube, which comprises a test tube (1), an inner sleeve (2), a one-way valve (3), a test tube cover (4) and a screw cap (5), the test tube (1) is provided with the inner sleeve (2), the bottom of the inner sleeve (2) is provided with at least two one-way valves (3) in opposite directions, the one-way valve (3) comprises a valve (301) and a valve piece (302), the test tube cover (4) covers the test tube (1), the screw cap (5) passes through the test tube cover (4) and forms a shaft joint relationship with the test tube cover (4), one end of the screw cap (5) located outside the test tube cover (4) is provided with a knob (501), one end of the screw cap (5) located inside the test tube cover (4) is provided with a screw rod (502), the screw rod (502) forms a threaded connection relationship with the opening end of the inner sleeve (2), and the test tube cover (4) is provided with a closable pressure relief hole (6).

2. A centrifuge tube from which supernatant or sediment can be extracted according to claim 1, characterized in that: The test tube (1) is provided with scale lines (8) on the side.

3. The centrifuge tube of claim 1, wherein: The valve piece (302) of the one-way valve (3) is made of elastic material.

4. A centrifuge tube from which supernatant or sediment can be extracted according to claim 3, characterized in that: The valve piece (302) of the one-way valve (3) is made of corrosion-resistant rubber material.

5. The centrifuge tube of claim 1, wherein: The pressure relief hole (6) is closed by a small cover (7) that can be screwed on the small hole.

6. A centrifuge tube from which an extractable supernatant or pellet can be obtained according to any one of claims 1 to 5, characterized in that: The knob (501) of the screw cap (5) is provided with anti-slip lines (9).

Images