Exosome concentration and purification equipment and system

Abstract

The utility model discloses exosome concentration and purification equipment and system, which comprises a shell, a concentration device and a control system which are arranged in the shell, and further comprises a purification device which comprises a purification pump, a purification column and a buffer solution tank, an inlet end of the purification pump is respectively connected with an outlet of the concentration device and the buffer solution tank through a first three-way electromagnetic valve, an outlet end of the purification pump is connected with an inlet of the purification column, and an outlet of the purification column is respectively connected with a purified liquid storage tank and a second waste liquid tank through a second three-way electromagnetic valve; a concentration pump of the concentration device and a purification pump of the purification device are arranged in the shell, a concentration filter of the concentration device and a purification column of the purification device are arranged on the outer side wall of the shell, and the concentration pump, the purification pump, the first three-way electromagnetic valve and the second three-way electromagnetic valve are electrically connected with the control system respectively. The device is simple in structure, integrates the functions of concentration and purification, and reduces the cost.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of biological technology, and specifically relates to an exosome concentration and purification device and system. BACKGROUND

[0002] Existing stem cell exosomes are widely used in the field of biomedicine, and stem cell exosomes are high-purity stem cell exosomes separated from biological samples, wherein stem cell culture medium is the main source of stem cell exosomes for treatment. In order to meet the needs of treatment applications, it is necessary to separate exosomes from a large amount of cell culture medium, but a large amount of cell culture medium is not conducive to the concentration and separation operation of exosomes. Therefore, for the actual application of exosomes, it is necessary to establish an efficient concentration and purification separation method to concentrate stem cell exosomes from the culture medium.

[0003] The existing stem cell exosome concentration method either only uses a single instrument to simply concentrate cell extracellular vesicles without purification function or needs to use multiple instruments in combination to complete the steps of concentration, purification, sterilization and the like. Moreover, in the concentration process, the exosomes or protein impurities will block the filter membrane, the filter membrane is difficult to reuse, resulting in time-consuming and high-cost filtration. Therefore, there is an urgent need to develop a stem cell exosome concentration and purification device that is efficient, low in cost and convenient to clean. SUMMARY

[0004] The utility model aims at the shortage of prior art, provides a kind of exosome concentration and purification device and system, simple structure, integrated concentration and purification function, low in cost, it is convenient to clean concentration filter.

[0005] One of the purposes of the utility model is to adopt the technical scheme that

[0006] An exosome concentration and purification device, comprising a shell, a concentration device and a control system arranged in the shell, the concentration device is provided with a concentration pump and a concentration filter, further comprising a purification device, the purification device comprises a purification pump, a purification column and a buffer tank, the inlet end of the purification pump is connected with the outlet of the concentration device and the buffer tank respectively through a first three-way electromagnetic valve, the outlet end of the purification pump is connected with the inlet of the purification column, the outlet of the purification column is used to connect a purified liquid storage tank and a second waste liquid tank respectively through a second three-way electromagnetic valve, the concentration pump of the concentration device and the purification pump of the purification device are arranged in the shell, the flow of the concentration pump of the concentration device is greater than the flow of the purification pump, the concentration filter of the concentration device and the purification column of the purification device are arranged on the outer wall of the shell, and the concentration pump, the purification pump, the first three-way electromagnetic valve and the second three-way electromagnetic valve are electrically connected with the control system respectively.

[0007] Further, the concentration device comprises a concentration pump, a concentration filter, a sampling valve, a proportional pinch valve and a device failure valve, the concentration filter is connected with the inlet of the stock solution tank through the concentration pump, the device failure valve is arranged at the inlet end of the concentration filter, the outlet end of the concentration filter is connected with the stock solution tank through a backflow pipeline, the sampling valve and the proportional pinch valve are arranged on the backflow pipeline, the inlet end of the concentration filter is provided with an inlet membrane pressure sensor, the outlet end of the concentration filter is provided with an outlet membrane pressure sensor, and the waste liquid port of the concentration filter extends out of the shell through a pipeline and is connected with the first waste liquid tank.

[0008] Further, the concentration pump adopts a large-flow peristaltic pump, the purification pump adopts a small-flow peristaltic pump, and the flow of the concentration pump of the concentration device is greater than that of the purification pump.

[0009] Further, a three-way backwashing water valve is further included, the concentration filter adopts a concentration column, a backwashing on-off valve is arranged on the concentration filter, the three-way backwashing water valve is arranged between the inlet end of the concentration filter and the device failure valve, the waste liquid port of the concentration filter is connected with a backwashing tank through a pipeline, and the three-way backwashing water valve is connected with a backwashing waste water tank.

[0010] Further, an alarm device is further included, the alarm device is electrically connected with the control system, and the alarm device is used for alarming and prompting when the pressure of the concentration device exceeds a preset pressure.

[0011] Further, a water pan is further included, the second three-way electromagnetic valve, the sampling valve, the device failure valve and the backwashing on-off valve are arranged on one side of the shell, and the water pan is arranged at the lower end of the second three-way electromagnetic valve, the sampling valve, the device failure valve and the backwashing on-off valve.

[0012] Further, the proportional pinch valve is an electric proportional valve, and the sampling valve, the device failure valve and the backwashing on-off valve are all manual valves.

[0013] Further, the control system is arranged at the upper end of the shell, and the control system is provided with a display screen.

[0014] The second purpose of the utility model discloses the technical scheme that adopts:

[0015] An exosome concentration and purification system, comprising a stock solution tank, a concentration and purification device and a first waste liquid tank, wherein the concentration and purification device adopts the exosome concentration and purification device described above.

[0016] Further, the lower end of the stock solution tank, the buffer tank and the first waste liquid tank is provided with a weighing device.

[0017] The above technical scheme has the following beneficial effects:

[0018] The utility model discloses simple structure, through with the concentration device and the purification device set up in a casing, form the single instrument of concentration and purification function, do not need many instruments, reduced cost.

[0019] Still include three -way backwash water valve, the concentration filter adopts concentration column, be equipped with backwash on -off valve on the concentration filter, three -way backwash water valve set up between the import end of concentration filter and equipment failure valve, the waste liquid mouth of concentration filter is connected with backwash jar through pipeline, three -way backwash water valve is used for connecting backwash waste water jar, and the concentration filter is cleaned up conveniently.

[0020] The concentration pump adopts a large flow peristaltic pump, and the volume is large before concentration and small after concentration, the purification pump adopts a small flow peristaltic pump, and the flow of the concentration pump of the concentration device is greater than that of the purification pump.

[0021] Still include water pan, second three -way electromagnetic valve, sampling valve, equipment failure valve and backwash on -off valve all set up in a side of casing, water pan set up in the lower end of second three -way electromagnetic valve, sampling valve, equipment failure valve and backwash on -off valve, prevent the leakage of connecting place through water pan.

[0022] The following further description is made in conjunction with the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF DRAWINGS

[0023] Fig. 1 It is structural schematic diagram of specific embodiment 1;

[0024] Fig. 2 It is working principle diagram of specific embodiment 1.

[0025] In the drawings, 1 is a casing, 2 is a concentration pump, 3 is a purification pump, 4 is a proportional pinch valve, 5 is a first three-way electromagnetic valve, 6 is a control system, 7 is a display screen, 8 is a purification column, 9 is a concentration column, 10 is a second three-way electromagnetic valve, 11 is a membrane inlet pressure sensor, 12 is a water pan, 13 is a stock solution tank, 14 is a sampling valve, 15 is an equipment failure valve, 16 is a first waste liquid tank, 17 is a buffer solution tank, 18 is a purified liquid storage tank, 19 is a second waste liquid tank, 20 is a membrane outlet pressure sensor, 21 is a three-way backwash water valve, and 22 is a backwash on-off valve. DETAILED DESCRIPTION SPECIFIC EMBODIMENT

[0026] See Figs. 1-2As shown, an exosome concentration purification device includes a shell 1, a concentration device, a purification device and a control system arranged in the shell, the concentration device is provided with a concentration pump and a concentration filter, the concentration device includes a concentration pump, a concentration filter, a sampling valve 14, a proportional pinch valve and a device failure valve 15, the concentration filter can be a concentration column or a flat plate filter membrane, the concentration filter is connected with the inlet of the stock solution tank 13 through the concentration pump, the device failure valve is arranged at the inlet end of the concentration pump, the outlet end of the concentration filter is connected with the stock solution cylinder through a backflow pipeline, the sampling valve and the proportional pinch valve are arranged on the backflow pipeline, the proportional pinch valve is an electric proportional valve, the inlet end of the concentration filter is provided with an inlet membrane pressure sensor, the outlet end of the concentration filter is provided with an outlet membrane pressure sensor 20, and the waste liquid port of the concentration filter extends out of the shell through a pipeline to connect a first waste liquid tank 16, and a on-off valve is arranged on the pipeline to shut off the first waste liquid tank 16. Further, the sampling valve, the device failure valve and the backwashing on-off valve are all manual valves.

[0027] The purification device includes a purification pump, a purification column and a buffer solution tank 17, the inlet end of the purification pump is connected with the outlet of the concentration device and the buffer solution tank through a first three-way electromagnetic valve respectively, the first three-way electromagnetic valve is connected with the concentration device through a purification pipeline, the first three-way electromagnetic valve is connected with the buffer solution tank through a buffer solution pipeline, and the outlet end of the purification pump is connected with the inlet of the purification column. The outlet of the purification column is connected with a purified liquid storage tank 18 and a second waste liquid tank 19 through a second three-way electromagnetic valve, the second three-way electromagnetic valve is connected with the second waste liquid tank 19 through a waste liquid pipeline, and the second three-way electromagnetic valve is connected with the purified liquid storage tank through a purification pipeline. The concentration pump of the concentration device and the purification pump of the purification device are arranged in the shell, the concentration pump and the purification pump are both peristaltic pumps, the flow rate of the concentration pump of the concentration device is greater than that of the purification pump, and the concentration filter of the concentration device and the purification column of the purification device are arranged on the outer wall of the shell. The concentration pump, the purification pump and the first three-way electromagnetic valve are electrically connected with the control system respectively.

[0028] The control system is arranged at the upper end of the shell, and the control system includes a single-chip microcomputer, a display screen and an input keyboard electrically connected with the single-chip microcomputer, the display screen is used for displaying pressure, and the input keyboard is used for inputting parameters.

[0029] In this specific embodiment: the thickening filter uses a thickening column, which is a hollow fiber membrane column. Possibly, it also includes a three-way backwash water valve 21. The thickening filter is equipped with a backwash shut-off valve 22, which is either a ball valve or a diaphragm valve. Ball valves are cheaper, while diaphragm valves are corrosion-resistant and mainly used for strong acids and alkalis. The three-way backwash water valve is located between the inlet end of the thickening filter and the equipment failure valve. The waste liquid outlet of the thickening filter is connected to the backwash tank via a pipeline. The three-way backwash water valve is used to connect to the backwash wastewater tank, which contains backwash liquid. During backwashing, the backwash wastewater tank is replaced by the first waste liquid tank 16.

[0030] Furthermore, it also includes an alarm device, which is electrically connected to the control system. The alarm device is used to detect if the pressure of the concentration device exceeds a preset pressure, and then issue an alarm. In this specific embodiment, the alarm device adopts an audible and visual alarm.

[0031] Furthermore, it also includes a water receiving tray, and the second three-way solenoid valve, sampling valve, equipment failure valve and backwash shut-off valve are all located on one side of the housing, with the water receiving tray located at the lower end of the second three-way solenoid valve, sampling valve, equipment failure valve and backwash shut-off valve.

[0032] An exosome concentration and purification system includes a stock solution tank, a concentration and purification device, and a first waste solution tank, wherein the concentration and purification device is the exosome concentration and purification device described above.

[0033] The lower ends of the stock solution tank, the buffer solution tank, and the first waste solution tank are all equipped with weighing devices to convert weight into volume. In this specific embodiment, the weighing device uses a load cell.

[0034] The working principle of this utility model:

[0035] 1. Set the concentration and purification parameters in the control system.

[0036] 2. The equipment starts the concentration pump, which pumps the raw material liquid in the raw liquid tank into the concentration filter (hollow fiber membrane column) through the inlet membrane pressure sensor. The waste liquid of the concentration filter is discharged from the waste liquid outlet and discharged into the first waste liquid tank. The remaining concentrate is discharged from the outlet and flows back to the raw material liquid tank after passing through the outlet membrane pressure sensor and the proportional clamp valve.

[0037] 3. The concentrator pump is a high-flow peristaltic pump. After startup, the flow rate is gradually increased until it reaches the shear flow rate of the concentrator filter, at which point the increase stops.

[0038] 4. The proportional pinch valve slowly clamps the pipeline, and the pipeline pressure gradually increases to the operating pressure of the concentrator filter.

[0039] 5. As the concentration proceeds, the concentration of the stock solution increases, the pipeline pressure increases, and the proportional pinch valve gradually releases to maintain the pressure at the concentration filter use pressure.

[0040] 6. When the proportional pinch valve is fully open, the concentration pump gradually reduces the flow to maintain the pressure at the concentration filter use pressure.

[0041] 7. When the weighing sensor at the lower end of the stock solution tank detects that the remaining weight reaches the set value, the concentration pump stops and the concentration stops.

[0042] 8. When the inlet membrane pressure sensor or the outlet membrane pressure sensor reaches the concentration filter overpressure value, the concentration pump stops and the audible and visual alarm sounds.

[0043] 9. After the concentration is completed and the concentration pump stops, the purification pump is started, the first three-way electromagnetic valve is switched to the purification pipeline, and the purification begins.

[0044] 10. The purification pump is a small flow peristaltic pump, which pumps the concentrated stock solution into the purification column at a set fixed flow rate, and the second three-way electromagnetic valve after the purification column is switched to the waste liquid pipeline connected to the second waste liquid tank to discharge the front section waste liquid.

[0045] 11. When the stock solution is fully pumped into the purification column, the first three-way electromagnetic valve before the pump is switched to the buffer solution pipeline connected to the buffer solution tank to pump the buffer solution into the purification column.

[0046] 12. When the volume of the stock solution and the buffer solution pumped by the purification pump reaches the set front section waste liquid volume, the second three-way electromagnetic valve after the purification column is switched to the purification pipeline to collect the liquid into the purified liquid storage tank, and the front section is divided into a front section, a middle section and a rear section according to the time.

[0047] 13. When the volume of the stock solution and the buffer solution pumped by the purification pump reaches the sum of the set front section waste liquid volume and the purified liquid volume, the purification pump stops, and the entire concentration and purification are completed.

Claims

1. An exosome concentration and purification device, comprising a housing, a concentration unit disposed within the housing, and a control system, wherein the concentration unit is equipped with a concentration pump and a concentration filter, characterized in that: It also includes a purification device, which includes a purification pump, a purification column, and a buffer tank. The inlet of the purification pump is connected to the outlet of the concentration device and the buffer tank via a first three-way solenoid valve. The outlet of the purification pump is connected to the inlet of the purification column. The outlet of the purification column is connected to a purified liquid storage tank and a waste liquid tank via a second three-way solenoid valve. The concentration pump of the concentration device and the purification pump of the purification device are disposed inside the housing. The concentration filter of the concentration device and the purification column of the purification device are disposed on the outer wall of the housing. The concentration pump, the purification pump, the first three-way solenoid valve, and the second three-way solenoid valve are electrically connected to the control system.

2. The exosome concentration and purification device according to claim 1, characterized in that: The concentration device includes a concentration pump, a concentration and sampling valve, a proportional clamp valve, and an equipment failure valve. The concentration filter is connected to the inlet of the raw liquid tank via the concentration pump. The equipment failure valve is located at the inlet end of the concentration filter. The outlet end of the concentration filter is connected to the raw liquid tank via a reflux pipe. The sampling valve and the proportional clamp valve are located on the reflux pipe. The inlet end of the concentration filter is equipped with an inlet membrane pressure sensor, and the outlet end of the concentration filter is equipped with an outlet membrane pressure sensor. The waste liquid outlet of the concentration filter extends out of the housing via a pipe to connect to the first waste liquid tank.

3. An exosome concentration and purification device according to claim 1 or 2, characterized in that: The concentration pump is a high-flow peristaltic pump, the purification pump is a low-flow peristaltic pump, and the flow rate of the concentration pump in the concentration device is greater than the flow rate of the purification pump.

4. The exosome concentration and purification device according to claim 2, characterized in that: It also includes a three-way backwash water valve. The thickening filter adopts a thickening column. The thickening filter is equipped with a backwash shut-off valve. The three-way backwash water valve is located between the inlet end of the thickening filter and the equipment fault valve. The waste liquid port of the thickening filter is connected to the backwash tank through a pipeline. The three-way backwash water valve is used to connect to the backwash wastewater tank.

5. The exosome concentration and purification device according to claim 2, characterized in that: It also includes an alarm device, which is electrically connected to the control system. The alarm device is used to detect if the pressure of the concentration device exceeds a preset pressure, and then issue an alarm.

6. The exosome concentration and purification device according to claim 2, characterized in that: It also includes a water receiving tray. The second three-way solenoid valve, sampling valve, equipment failure valve and backwash shut-off valve are all located on one side of the housing. The water receiving tray is located at the lower end of the second three-way solenoid valve, sampling valve, equipment failure valve and backwash shut-off valve.

7. The exosome concentration and purification device according to claim 2, characterized in that: The proportional pinch valve is an electric proportional valve, while the sampling valve, equipment failure valve, and backwash shut-off valve are all manual valves.

8. An exosome concentration and purification device according to claim 1 or 2, characterized in that: The control system is located at the upper end of the housing, and the control system is equipped with a display screen.

9. An exosome concentration and purification system, comprising a stock solution tank, a concentration and purification device, and a first waste solution tank, characterized in that: The concentration and purification equipment is the exosome concentration and purification equipment according to any one of claims 1 to 8.

10. The exosome concentration and purification system according to claim 9, characterized in that: The lower ends of the stock solution tank, the buffer solution tank, and the first waste solution tank are all equipped with weighing devices.

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