Sterile sampling device for corrugated pipe of bioreactor

Abstract

The utility model discloses a bioreactor corrugated pipe sterile sampling device, and relates to the field of biological culture sampling, the bioreactor corrugated pipe sterile sampling device comprises a reaction tank and a reaction tank upper cover fixed at an opening of the reaction tank, a winding roll film with a carrier is placed in the reaction tank, and a sampling strip is inserted between the winding roll films; a corrugated pipe for inserting one end of the sampling strip is arranged on the surface of the reaction tank upper cover, the top end in the corrugated pipe is fixedly connected with the end, away from the winding film, of the sampling strip, when the corrugated pipe is stretched, the sampling strip located in the winding film is completely located in the shearing section to achieve sampling, and the surface of the shearing section is sleeved with a sealing part used for sealing the shearing section; when the corrugated pipe is compressed, a clamping frame for limiting is arranged on the surface, and the corrugated pipe can be pulled to be unfolded when the clamping frame is taken down. Therefore, the reaction tank cannot be polluted by external air during sampling by the sampling device, the sampling detection precision is high, and the operation is also convenient.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biological culture sampling, in particular to a biological reactor bellows aseptic sampling device. BACKGROUND

[0002] Biological reactors achieve fixation, culture and sampling, thereby achieving efficient biological treatment and analysis. In a biological fixed bed system, a sampling device is a key component that is responsible for extracting biological samples or reaction products from the fixed bed for subsequent analysis and research, and thus requires sampling to determine the culture conditions of the organism.

[0003] The existing sampling device opens the biological culture device by rotating the threads to sample, but opening the culture device causes the cultured organisms to come into contact with the outside air, resulting in contamination of the biological sample. CONTENT OF THE UTILITY MODEL

[0004] In order to improve the problem of contamination of biological culture caused by sampling, the present application provides a biological reactor bellows aseptic sampling device.

[0005] The biological reactor bellows aseptic sampling device provided by the present application adopts the following technical solution:

[0006] A biological reactor bellows aseptic sampling device, comprising a reactor tank and a reactor tank upper cover fixed at the opening of the reactor tank, the inside of the reactor tank is placed with a winding film for carriers, a sampling strip is inserted into the inside of the winding film, so that the organism grows on the surface of the sampling strip and is used to evaluate the growth of the organism on the surface of the winding film;

[0007] The reactor tank upper cover is sealingly provided with a bellows for inserting one end of the sampling strip, the inside top end of the bellows is fixedly connected with the end of the sampling strip away from the winding film, and the end of the bellows is provided with a shear section between the reactor tank upper cover;

[0008] When the bellows is elongated, the sampling strip inside the winding film is completely located inside the shear section, thereby achieving sampling, and the surface of the shear section is provided with a fixing clamp for sealing the shear section;

[0009] When the bellows is compressed, the surface is provided with a clamping frame for limiting, and the clamping frame can be removed to pull out the bellows.

[0010] By adopting the above technical solution, the clamping frame is used to prevent the bellows from popping out, the sampling strip is pulled out of the reactor tank by the bellows, and the sealing fixation of the fixing clamp can avoid the entry of external air and impurities into the reactor tank to contaminate the organism and cells during sampling, effectively improving the sampling effect. At the same time, the sampling strip is also protected by the bellows, and the length of the shear section is set to at least 1 centimeter.

[0011] Preferably, the other end of the bellows is provided as a fixed section, the fixed section is a sealed structure, and one end of the sampling strip is fixed to the top inside the fixed section.

[0012] By adopting the above technical scheme, the fixed section can be fused and fixed with the sampling strip and realize sealing, so as to avoid air entering the reaction tank and facilitate pulling the sampling strip.

[0013] Preferably, the bellows is made of a sterile material and has a length greater than that of the sampling strip.

[0014] By adopting the above technical scheme, the bellows can completely accommodate the sampling strip to achieve protection and facilitate shearing of the bellows, and the bellows is made of TPE or EVA.

[0015] Preferably, the sealing part is provided as a fixed clamp, the fixed clamp includes a clamp body, and the middle part and one end of the clamp body are respectively provided with an upper through hole and a lower through hole for inserting the shearing section.

[0016] By adopting the above technical scheme, the upper through hole and the lower through hole can facilitate insertion of the bellows and fix the fixed clamp on the bellows for subsequent use.

[0017] Preferably, opposite sides of the clamp body are respectively provided with abutting protrusions for extruding the shearing section and realizing sealing.

[0018] By adopting the above technical scheme, the opposite surfaces of the abutting protrusions are provided as arc chamfers to avoid damaging the bellows.

[0019] Preferably, a clamping groove is formed on one side of the clamp body surface located at the lower through hole for inserting the end of the clamp body away from the lower through hole, and when the end of the clamp body away from the lower through hole is inserted into the clamping groove, the two abutting protrusions are close to each other to extrude and seal the shearing section.

[0020] By adopting the above technical scheme, the clamping groove and one end of the clamp body are fixed to realize clamping and fixing, so that the part of the bellows cut off is always in a sealed state.

[0021] Preferably, the surface of the upper cover of the reaction tank is equally provided with a plurality of joints in communication with the reaction tank, the joints are inserted and fixed with the shearing section of the bellows, the sampling strip penetrates the joints, and one end of the sampling strip located inside the reaction tank is attached to the surface of the winding film.

[0022] By adopting the above technical scheme, the joints can be quickly connected and fixed with the bellows, which is convenient for installation and use.

[0023] Preferably, the middle part of the upper cover of the reaction tank is fixedly provided with a liquid injection pipe in communication with the reaction tank.

[0024] By adopting the technical scheme, the culture solution can be injected into the reaction tank through the liquid injection pipe for biological and cell growth.

[0025] To sum up, the present application has at least one of the following beneficial technical effects:

[0026] 1. Pull the corrugated pipe to move the sampling strip out of the reaction tank and into the corrugated pipe, then clamp and seal the cutting section by the fixed clamp to avoid air entering the reaction tank, then cut the corrugated pipe at the lower end of the sampling strip and the upper end of the fixed clamp to realize the sampling effect. This sampling will not cause the reaction tank to be contaminated by external air, and the sampling detection accuracy is high and the operation is convenient. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 is a schematic diagram of the present application as a whole;

[0028] Figure 2 is a schematic diagram of the present application as a side view;

[0029] Figure 3 is a schematic diagram of the present application as a fixed clamp, corrugated pipe and sampling strip separation.

[0030] Reference signs: 1, reaction tank; 2, reaction tank upper cover; 3, joint; 4, liquid injection pipe;

[0031] 5, fixed clamp; 51, clamp body; 52, upper perforation; 53, lower perforation; 54, clamping groove; 55, abutting protrusion;

[0032] 6, clamping frame; 7, corrugated pipe; 8, sampling strip; 9, cutting section; 10, fixed section. DETAILED DESCRIPTION

[0033] The following will be described in detail in combination with the accompanying Figures 1-3 The present application will be further described in detail.

[0034] The present application discloses a kind of biological reactor corrugated pipe aseptic sampling device.

[0035] Refer to Figure 1The utility model provides a kind of bioreactor bellows aseptic sampling device, including reactor 1 and the upper cover of reactor 2 fixed on the upper end of reactor 1, the size of upper cover of reactor 2 is greater than the diameter of reactor 1, and the outside of reactor 1 is also closed by container, and the specific structure connection relationship of the structure is not detailed, at least four joints 3 are equally divided and arranged on the upper end of upper cover of reactor 2, four joints 3 are communicated with reactor 1, joint 3 is inserted and fixed with the shear section 9 of bellows 7 and can realize disassembly, sampling strip 8 is inserted through joint 3 and located in the middle of the inner chamber of joint 3, and liquid injection pipe 4 is fixed on the upper end of upper cover of reactor 2, which is communicated with reactor 1 for injecting culture solution, and sealing structure is also provided on liquid injection pipe 4.

[0036] Bellows 7 can be installed on each joint 3 to realize sampling at multiple positions inside reactor 1, increase sampling accuracy, and container is sleeved on the surface of reactor 1 and abuts against the lower end of upper cover of reactor 2, then connected and fixed by bolt or other fixing mode to form a whole.

[0037] Refer to Figure 2 、 Figure 3 Carrier winding film is placed inside reactor 1, winding film is used as cell culture carrier structure and is mature technology, and the application will not be detailed, sampling strip 8 is inserted between winding film, one end of sampling strip 8 can be attached to the surface of winding film inside reactor 1, one end of sampling strip 8 extends to the lower end of winding film, so that cells grow on sampling strip 8, the material of sampling strip 8 is the same as that of winding film, so that the cells grow on the surface of sampling strip 8, and the growth of cells on the surface of winding film is evaluated; the upper end of bellows 7 is provided as fixed section 10, the lower end of bellows 7 and upper cover of reactor 2 are provided as shear section 9, the end of fixed section 10 is provided as sealing, and the end of sampling strip 8 away from winding film is fixed to the top inside fixed section 10, bellows 7 is made of sterile material, and the length of bellows 7 is greater than the length of sampling strip 8, so that sampling strip 8 is completely located inside bellows 7, and one end of sampling strip 8 is located inside bellows 7.

[0038] The user inserts one end of sampling strip 8 into winding film to culture cells with winding film, so that the growth of cells on sampling strip 8 is representative of the growth of cells on the surface of winding film, then one end of sampling strip 8 is inserted through joint 3 and into bellows 7, then the shear section 9 of bellows 7 is inserted into joint 3, then sampling strip 8 is straightened, and then the fixed section 10 of bellows 7 and one end of sampling strip 8 are fixed together by external melting shear machine to realize overall fixation.

[0039] When bellows 7 is elongated, sampling strip 8 located inside winding film is completely located inside shear section 9 to realize sampling.

[0040] Referring to Figure 2 , Figure 3 The sealing part is provided with a fixing clamp 5, and the surface of the shearing section 9 is sleeved with the fixing clamp 5 for sealing the shearing section 9. The fixing clamp 5 can also adopt an existing product, such as a Robert pipe clamp. The fixing clamp 5 comprises a clamp body 51, the middle part of the clamp body 51 is provided with an upper through hole 52 for inserting the shearing section 9, one end of the clamp body 51 is provided with a lower through hole 53 for inserting the shearing section 9, and the opposite sides of the clamp body 51 are both fixedly provided with abutting protrusions 55. The surface of the clamp body 51 is provided with a clamping groove 54 on the side of the lower through hole 53. The end of the clamp body 51 away from the lower through hole 53 is inserted into the clamping groove 54 and is clamped and fixed. When the end of the clamp body 51 away from the lower through hole 53 is inserted into the clamping groove 54, the two abutting protrusions 55 are close to each other, press the shearing section 9 and realize sealing. The surface of the bellows 7 is provided with a clamping frame 6 for limiting when the bellows 7 is compressed. The clamping frame 6 is provided with an "U" shape and is provided with an arc-shaped groove at both ends and is fixed to the surface of the bellows 7 under the action of friction. The clamping frame 6 can be removed to pull the bellows 7 to expand. The sealing part can also use a pipe sealing machine to heat and melt to seal the shearing section 9.

[0041] When sampling, the user first removes the clamping frame 6, then holds the connection between the shearing section 9 and the joint 3 with one hand and does not separate, then stretches the bellows 7 to stretch the bellows 7, and then drives the sampling strip 8 to slowly move out of the reaction tank 1, until the end of the sampling strip 8 for culture enters the shearing section 9, and the end of the sampling strip 8 for culture has a distance from the joint 3, the distance is used for shearing, then the user moves the fixing clamp 5 to the position of the shearing section 9 at the lower end of the sampling strip 8, then the user holds the two ends of the clamp body 51 with his hand, and clamps one end of the clamp body 51 into the clamping groove 54 to realize fixation. In this process, the two abutting protrusions 55 are close to each other, press the shearing section 9 and deform to realize sealing, so as to avoid the reaction tank 1 from being connected with the outside world. Then the user takes away the cut bellows 7 to realize sampling.

[0042] The implementation principle of the biological reactor bellows sterile sampling device is as follows: when sampling, the user first removes the clamping frame 6 from the surface of the bellows 7, then the user pulls the fixed section 10 of the bellows 7 with his hand, and then drives the sampling strip 8 to move, so that the sampling strip 8 moves out of the reaction tank 1. When the bellows 7 moves to the maximum distance, the sampling strip 8 is completely located in the bellows 7. Then the user moves the fixing clamp 5 to the position between the lower end of the sampling strip 8 and the joint 3, and presses the fixing clamp 5 to seal and fix a part of the shearing section 9. Then the user uses scissors to cut the shearing section 9 at the upper end of the fixing clamp 5, so that the sampling strip 8 is separated from the upper cover 2 of the reaction tank, and sampling is realized.

[0043] The above merely describes optional embodiments of the present disclosure, and is not intended to limit the present disclosure. The present disclosure can have various modifications and changes for those skilled in the art. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present disclosure shall be included in the protection scope of the present disclosure.

Claims

1. A bioreactor bellows aseptic sampling device, characterized by: The utility model provides a kind of reaction tank and its cover, including reaction tank (1) and the reaction tank upper cover (2) fixed at the opening of reaction tank (1), the inside of the reaction tank (1) is placed for the winding film of carrier, sampling strip (8) is inserted in the winding film; The upper end of the reaction tank upper cover (2) is sealingly provided with a bellows (7) for inserting one end of the sampling strip (8), the inner top end of the bellows (7) is fixedly connected with the end of the sampling strip (8) away from the winding film, and a shear section (9) is provided between one end of the bellows (7) and the reaction tank upper cover (2). When the bellows (7) is elongated, the sampling strip (8) located inside the winding film is completely located inside the shear section (9), thereby realizing sampling. The surface of the shear section (9) is provided with a sealing portion for sealing the shear section (9).

2. A bioreactor bellows aseptic sampling device according to claim 1, characterized in that: When the bellows (7) is compressed, the surface is provided with a clamping frame (6) for limiting, and the clamping frame (6) can be removed to expand the bellows (7).

3. A bioreactor bellows aseptic sampling device according to claim 1, characterized in that: The other end of the bellows (7) is provided as a fixed section (10), the fixed section (10) is sealed, and one end of the sampling strip (8) is fixed to the top inside the fixed section (10).

4. A bioreactor bellows aseptic sampling device according to claim 1, characterized in that: The bellows (7) is made of sterile material and has a length greater than that of the sampling strip (8) after expansion.

5. A bioreactor bellows aseptic sampling device according to claim 4, wherein: The sealing portion is provided as a fixed clamp (5), which includes a clamp body (51), and the middle part and one end of the clamp body (51) are respectively provided with an upper through hole (52) and a lower through hole (53) for inserting the shear section (9).

6. A bioreactor bellows aseptic sampling device according to claim 5, wherein: The opposite sides of the clamp body (51) are fixedly provided with abutting protrusions (55) for extruding the shear section (9) and realizing sealing.

7. A bioreactor bellows aseptic sampling device according to claim 1, wherein: The surface of the clamp body (51) is provided with a clamping groove (54) on one side of the lower through hole (53) for inserting the end of the clamp body (51) away from the lower through hole (53).

8. A bioreactor bellows aseptic sampling device according to claim 1, wherein: The surface of the reaction tank upper cover (2) is equally provided with a plurality of joints (3) communicating with the reaction tank (1), the joints (3) are fixedly connected with the shear section (9) of the bellows (7), the sampling strip (8) penetrates the joint (3), and one end of the sampling strip (8) located inside the reaction tank (1) is attached to the surface of the winding film. The middle part of the reaction tank upper cover (2) is fixedly provided with a liquid injection pipe (4) communicating with the reaction tank (1).

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