Separation device for single cell sequencing

Abstract

The utility model is applicable to the technical field of single cell sequencing, and provides a separation device for single cell sequencing, which comprises a separation frame arranged on a rack and used for separating single cells, and a separation mechanism used for driving the separation frame to rotate to separate the single cells is arranged on the rack and the separation frame; the mixing vessel is arranged on the rack and is used for mixing tissues containing single cells and a solvent; the separation hopper is assembled on the rack, and a filter screen for separating a solution containing single-cell tissues is arranged in the separation hopper; and the extruding mechanism is arranged on the rack and is used for extruding and mixing the tissue containing the single cells and the solution in the mixing vessel. According to the separation device for single cell sequencing, provided by the scheme, single cell separation operation can be carried out, a worker can be helped to carry out pretreatment operation on a single cell solution, the operation burden of the worker is effectively reduced, and convenience is provided for the worker.

Description

TECHNICAL FIELD

[0001] The utility model belongs to single cell sequencing technical field especially, it relates to a kind of separating device for single cell sequencing. BACKGROUND

[0002] When single cell sequencing is carried out, single cell needs to be separated and handled, so the corresponding separating device for single cell sequencing needs to be used.

[0003] When staff carries out single cell separation processing, single cell is separated by centrifuging solution containing single cell and corresponding solvent, but before separation, staff needs to mix tissue containing cell and corresponding solvent, and the tissue needs to be repeatedly crushed during mixing to make tissue and solvent fully mixed, then it is filtered to obtain solution containing single cell, and some separating devices for single cell sequencing cannot help staff to carry out these operations, and staff needs to manually operate, which undoubtedly increases the operation burden of staff when staff carries out a large number of experimental operations every day. UTILITY MODEL CONTENT

[0004] The utility model provides a kind of separating device for single cell sequencing, to solve the problem of the separating device for single cell sequencing currently used in the above background art is more limited.

[0005] To solve the above problems, the utility model is realized as follows: a kind of separating device for single cell sequencing, comprising: separating frame for separating single cell is arranged on rack, the rack and the separating frame are provided with separating mechanism for driving the separating frame rotation to separate single cell;Mixing dish for mixing tissue containing single cell and solvent is arranged on the rack;Separating hopper is assembled on the rack, and the separating hopper is provided with filter screen for separating single cell tissue solution;Extrusion mechanism for extruding and mixing tissue containing single cell and solution in mixing dish is arranged on the rack.

[0006] Preferably, the extrusion mechanism includes: telescopic rod fixedly installed on the rack;Extrusion rod for extruding tissue containing single cell in the mixing dish is arranged on the output shaft of the telescopic rod.

[0007] Preferably, the rack and the mixing dish are provided with a pouring mechanism for pouring the single-cell tissue solution mixed in the mixing dish into the separation hopper, the pouring mechanism comprising: a first servo motor fixedly installed on the rack; two installation shafts rotatably installed on the rack, one end of one of the installation shafts being fixedly connected with an output shaft of the first servo motor; and a mounting frame fixedly installed on the two installation shafts, the mounting frame being threadedly connected with the mixing dish.

[0008] Preferably, the separation mechanism comprises: a second servo motor fixedly installed on the rack; a connecting shaft rotatably installed on the rack, a bottom end of the connecting shaft being fixedly connected with an output shaft of the second servo motor, and a top end of the connecting shaft being fixedly connected with a bottom of the separation frame.

[0009] Preferably, a chute is formed in the rack, the chute is annularly arranged, and a plurality of sliding blocks are fixedly installed on the bottom of the separation frame, the sliding blocks being slidably connected with the chute.

[0010] Preferably, a plurality of placement grooves for placing centrifuge tubes used for containing single-cell solution are formed in the separation frame, and a plurality of positioning frames are threadedly installed on the separation frame, the positioning frames being used for abuttingly positioning the centrifuge tubes placed in the placement grooves.

[0011] Preferably, a threaded frame is fixedly installed on an output shaft of the telescopic rod, and a threaded block is fixedly installed on a top end of the extrusion rod, the threaded block being threadedly connected with the threaded frame.

[0012] Preferably, a placement frame is arranged on the rack, the placement frame being used for supporting and placing the separation hopper and the centrifuge tubes used for containing single-cell solution.

[0013] Compared with the related art, the separation device for single-cell sequencing has the following beneficial effects:

[0014] Compared with the prior art, the separation device for single-cell sequencing can perform single-cell separation operation, and can help the staff to perform pretreatment operation on the single-cell solution, effectively reducing the operation burden of the staff, and providing convenience for the staff. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 is a front view structural schematic diagram of the separation device for single-cell sequencing provided by the utility model;

[0016] Figure 2 is a front view structural schematic diagram of the separation device for single-cell sequencing provided by the utility model;

[0017] Figure 3 is aFigure 2 An enlarged structural schematic view of part A shown in FIG.

[0018] Figure 4 For Figure 2 An enlarged structural schematic view of part B shown in FIG.

[0019] The figure mark: 1, frame; 2, separation frame; 3, mixing dish; 4, separation hopper; 5, filter screen; 6, telescopic rod; 7, extrusion rod; 8, first servo motor; 9, mounting shaft; 10, mounting frame; 11, second servo motor; 12, connecting shaft; 13, sliding chute; 14, sliding block; 15, positioning frame; 16, placing frame. DETAILED DESCRIPTION

[0020] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used in the description herein is for describing particular embodiments only and is not intended to be limiting of the application; the description and the drawings are to be regarded as illustrative in nature and are not intended to limit the application; the terminology used in the description and the claims of the present application and the above description of the drawings includes the terms specifically mentioned above as well as their derivatives.

[0021] Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase "in an embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are they necessarily all directed to the same embodiment, or to a single alternative embodiment. It is explicitly contemplated that embodiments described herein can be combined with each other.

[0022] The utility model discloses an embodiment provides a kind of single cell sequencing with separation device, such as Figures 1-4As shown, the single cell separation device comprises a separation rack 2 provided on a rack 1 for separating single cells, the rack 1 and the separation rack 2 are provided with a separation mechanism for driving the separation rack 2 to rotate for single cell separation; a mixing dish 3 provided on the rack 1 for mixing single cell-containing tissue and solvent; a separation hopper 4 assembled on the rack 1, the separation hopper 4 is provided with a filter screen 5 for separating single cell-containing tissue solution; and a squeezing mechanism provided on the rack 1 for squeezing and mixing the single cell-containing tissue and solution in the mixing dish 3.

[0023] In this embodiment, first, the side of the device is provided with a controller, the whole device can be controlled by the controller, the working principle of the controller is prior art, and details are not repeated here, when performing the corresponding single cell separation operation, first, the worker puts the single cell-containing tissue and the corresponding solvent into the mixing dish 3, and then starts the squeezing mechanism to continuously squeeze the single cell-containing tissue in the mixing dish 3 back and forth through the controller, so that the single cell-containing tissue and the solvent are fully mixed, and then the mixed solution is poured into the separation hopper 4, and the single cell-containing solution is filtered and impurities are removed through the filter screen 5, and the single cell-containing solution is discharged into the centrifugal tube after filtering and impurities are removed, so that the corresponding single cell solution is obtained, and these pretreatment operations do not need to be operated by the worker, effectively reducing the operation burden of the worker, and providing convenience for the worker, then the worker places the centrifugal tube containing the single cell solution on the separation rack 2, and starts the separation mechanism to drive the centrifugal tube on the separation rack 2 to rotate through the controller, so as to perform the single cell separation operation, and then facilitate the worker to perform the single cell sequencing work, through the whole device, the single cell separation operation can be performed, and the worker can be helped to perform the single cell solution pretreatment operation, effectively reducing the operation burden of the worker, and providing convenience for the worker.

[0024] In further preferred embodiments of the utility model, the squeezing mechanism comprises: a telescopic rod 6 fixedly installed on the rack 1; and a squeezing rod 7 provided on an output shaft of the telescopic rod 6 for squeezing the single cell-containing tissue in the mixing dish 3.

[0025] In this embodiment, when the squeezing mechanism is used, the telescopic rod 6 drives the squeezing rod 7 to move back and forth up and down through the controller, so as to continuously squeeze the single cell-containing tissue in the mixing dish 3 back and forth, so that the single cell-containing tissue and the solvent are fully mixed, and the operation burden of the worker is reduced, and convenience is provided for the worker.

[0026] The utility model further preferable embodiment, the rack 1 and the mixing dish 3 are provided with the pouring mechanism for pouring the single cell tissue solution mixed in the mixing dish 3 to the separating hopper 4, the pouring mechanism includes: the first servo motor 8 of fixed mounting on the rack 1, two installation shafts 9 of rotation installation on the rack 1, one end of one of the installation shaft 9 and the output shaft of the first servo motor 8 fixed connection, the mounting bracket 10 of fixed installation on two installation shafts 9, the mounting bracket 10 and the mixing dish 3 are threadedly connected.

[0027] In this embodiment, after the extrusion rod 7 continuously back and forth extrudes the single cell containing tissue in the mixing dish 3, the controller starts the first servo motor 8 to drive the installation shaft 9 to rotate by a certain angle, thereby tilting the mixing dish 3, pouring the single cell containing solution in the mixing dish 3 into the separating hopper 4, and filtering the single cell containing solution through the filter screen 5 to remove impurities. After filtering and removing impurities, the single cell containing solution is discharged into the centrifugal tube, thereby obtaining the corresponding single cell solution. These pretreatment operations do not need to be operated by the staff, effectively reducing the operation burden of the staff, and providing convenience for the staff. Then the staff places the centrifugal tube containing the single cell solution on the separating frame 2 to perform the separation operation.

[0028] The utility model further preferable embodiment, the separating mechanism includes: the second servo motor 11 of fixed installation on the rack 1, the connecting shaft 12 of rotation installation on the rack 1, the bottom end of the connecting shaft 12 and the output shaft of the second servo motor 11 fixed connection, and the top end of the connecting shaft 12 and the bottom of the separating frame 2 fixed connection.

[0029] In this embodiment, when using the separating mechanism, the second servo motor 11 is started to drive the connecting shaft 12 to rotate, thereby driving the single cell containing solution in the centrifugal tube on the separating frame 2 to rotate and centrifuge, thereby performing single cell separation operation, and facilitating the staff to perform single cell sequencing work. The rotation speed of the second servo motor 11 can be controlled by the controller.

[0030] The utility model further preferable embodiment, the rack 1 is set up with the chute 13, the chute 13 is annularly set up, the bottom of the separating frame 2 is fixedly installed with a plurality of sliding blocks 14, and the plurality of sliding blocks 14 are slidably connected with the chute 13.

[0031] In this embodiment, when the separating frame 2 rotates, the separating frame 2 drives the sliding block 14 to rotate in the chute 13, thereby improving the stability of the separating frame 2 when rotating.

[0032] The further preferable embodiment of the utility model further provides a plurality of positioning frames 15 are screw -threaded on the separation frame 2, and the positioning frame 15 is used to the centrifugal tube that places in the placing groove is positioned by resisting.

[0033] In the embodiment, after placing the centrifugal tube in the placing groove on the separation frame 2, the positioning frame 15 is screw -threaded on the separation frame 2, and the positioning frame 15 is positioned by resisting the centrifugal tube placed in the placing groove, so that the solution in the centrifugal tube is conveniently centrifuged.

[0034] The further preferable embodiment of the utility model further provides that the output shaft of telescopic link 6 is fixedly installed with threaded frame, the top end of extruding rod 7 is fixedly installed with threaded block, and the threaded block is screw -threaded with threaded frame.

[0035] In the embodiment, the telescopic link 6 and extruding rod 7 are fixedly connected by the cooperation of threaded frame and threaded block, and the extruding rod 7 is conveniently disassembled and cleaned.

[0036] The further preferable embodiment of the utility model further provides that the rack 1 is provided with placing rack 16, and the placing rack 16 is used to support and place the separation hopper 4 and the centrifugal tube for containing single cell solution.

[0037] In the embodiment, the separation hopper 4 and the centrifugal tube for containing single cell solution are conveniently supported and placed by placing rack 16.

[0038] Compared with the related art, the whole device can be used for single cell separation operation, and can help the staff to pretreat single cell solution, effectively reduce the operation burden of the staff, and provide convenience for the staff.

[0039] In several embodiments provided in the application, it should be understood that the disclosed device can be realized by other ways.

[0040] The above examples are only used to illustrate the technical solutions of the present application, and are not intended to limit the protection scope of the present application. Obviously, the described examples are only some of the embodiments of the present application, not all the embodiments. Based on these examples, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of the present application. Although the present application has been described in detail with reference to the above examples, those of ordinary skill in the art can still combine, add or delete the features in the embodiments of the present application according to the circumstances without creative labor, so as to obtain different other technical solutions which do not deviate from the concept of the present application in essence, and these technical solutions also fall within the scope of the present application.

Claims

1. A separation device for single-cell sequencing, characterized in that, include: A separation rack (2) for separating single cells is set on a frame (1), and a separation mechanism for driving the separation rack (2) to rotate to separate single cells is provided on the frame (1) and the separation rack (2); A mixing dish (3) is set on the rack (1) for mixing tissues containing single cells and solvents; A separation hopper (4) is mounted on the frame (1), and a filter screen (5) is provided inside the separation hopper (4) for separating solutions containing single-cell tissues; An extrusion mechanism is installed on the frame (1) for extruding and mixing tissues and solutions containing single cells in a mixing dish (3).

2. The separation device for single-cell sequencing as described in claim 1, characterized in that, The extrusion mechanism includes: Telescopic rod (6) fixedly installed on the frame (1); A squeezing rod (7) is provided on the output shaft of the telescopic rod (6) for squeezing the tissue containing single cells in the mixing dish (3).

3. The separation device for single-cell sequencing as described in claim 1, characterized in that, The frame (1) and the mixing dish (3) are provided with a pouring mechanism for pouring the single-cell tissue solution mixed in the mixing dish (3) into the separation hopper (4), the pouring mechanism comprising: A first servo motor (8) is fixedly mounted on the frame (1); Two mounting shafts (9) are rotated and mounted on the frame (1), one end of which is fixedly connected to the output shaft of the first servo motor (8); A mounting bracket (10) is fixedly mounted on the two mounting shafts (9), and the mounting bracket (10) is threadedly connected to the mixing dish (3).

4. The separation device for single-cell sequencing as described in claim 1, characterized in that, The separation mechanism includes: A second servo motor (11) is fixedly mounted on the frame (1); Rotate the connecting shaft (12) mounted on the frame (1). The bottom end of the connecting shaft (12) is fixedly connected to the output shaft of the second servo motor (11), and the top end of the connecting shaft (12) is fixedly connected to the bottom of the separation frame (2).

5. The separation device for single-cell sequencing as described in claim 1, characterized in that, The frame (1) is provided with a sliding groove (13), which is arranged in a ring. Multiple sliders (14) are fixedly installed at the bottom of the separation frame (2), and the multiple sliders (14) are slidably connected to the sliding groove (13).

6. The separation device for single-cell sequencing as described in claim 1, characterized in that, The separation rack (2) is provided with multiple placement slots for placing centrifuge tubes containing single-cell solutions, and the separation rack (2) is threaded with multiple positioning frames (15), which are used to position the centrifuge tubes placed in the placement slots.

7. The separation device for single-cell sequencing as described in claim 2, characterized in that, A threaded bracket is fixedly installed on the output shaft of the telescopic rod (6), and a threaded block is fixedly installed on the top end of the extrusion rod (7). The threaded block and the threaded bracket are threadedly connected.

8. The separation device for single-cell sequencing as described in claim 1, characterized in that, The rack (1) is provided with a placement rack (16) for supporting and placing the separation bucket (4) and centrifuge tubes for holding single-cell solutions.

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