Enzymolysis reaction device

Abstract

The utility model discloses an enzymolysis reaction device which comprises a constant-temperature box, a crank sliding rod assembly is arranged at the bottom of the inner side of the constant-temperature box, guide rods are arranged at the front end and the rear end of the bottom of the inner side of the constant-temperature box, oscillation discs are installed on the guide rods, and the oscillation discs can move left and right on the guide rods. The top of the crank sliding rod assembly is connected with the oscillation disc. A plurality of trays are arranged on the oscillation disc from top to bottom, and the trays are detachably connected with the oscillation disc. According to the utility model, the crank sliding rod assembly is arranged in the constant-temperature box and can drive the oscillation disc to shake left and right back and forth; a plurality of trays can be mounted at the top of the oscillation disc, and substrates needing to react or containers filled with the substrates can be placed in the trays; under the action of the oscillating disc, reactants in the tray can oscillate back and forth, so that full contact between enzyme and a substrate is ensured; and under the action of the guide rod, the oscillation disc is stable enough.

Description

TECHNICAL FIELD

[0001] The utility model belongs to enzymolysis reaction equipment technical field, concretely relates to an enzymolysis reaction device. BACKGROUND

[0002] Flavonoid glycoside compound synthesis needs to use toxic reagent, and enzyme catalysis method can green, high selectivity glycosidic bond is synthesized, avoids side reaction. Therefore in the synthesis process of flavonoid glycoside compound often needs to use enzymolysis reaction device.

[0003] In the prior art, the Chinese patent with publication number CN218710529U discloses an enzymolysis reaction device, which comprises a reaction box, the inner wall of the reaction box is fixedly connected with a carrier plate, the upper surface of the carrier plate is fixedly embedded with a collecting hopper, the bottom surface of the collecting hopper is fixedly embedded with a first bearing, the bottom surface of the collecting hopper is provided with a plurality of leakage holes arranged in a ring shape, the inner wall of the reaction box is fixedly connected with a partition plate, the upper surface of the partition plate is fixedly embedded with a second bearing, and the inner bottom wall of the reaction box is provided with a motor, and the output end of the motor is fixedly connected with a rotating rod. The carrier plate, the collecting hopper, the motor, the rotating rod and the stirring rod are matched, the collecting hopper can hold reagents, the motor can drive the rotating rod to rotate, the stirring plate can stir the reagents in the collecting hopper, the reagents can uniformly fall downward from the leakage holes, and the stirring rod can uniformly stir the reagents, so that the efficiency of enzymolysis is improved.

[0004] The enzymolysis reaction device provided by the above-mentioned patent mainly provides a reaction container. However, in fact, different equipment is used for different enzymolysis reactions. For example, in the process of synthesizing flavonoid glycoside compounds by enzyme method, glycosylation reaction will use β-glucosidase or glycosyltransferase to transfer rutinose to the 7-OH position of flavonoid nucleus. The reaction conditions are as follows: the reaction is carried out in a constant temperature shaking incubator at a temperature of 40°C, in a pH 5.5 buffer solution, for 12-24 hours. The constant temperature shaking incubator controls the reaction temperature (usually 25-50°C) and provides homogeneous stirring, ensuring that the enzyme and the substrate are in sufficient contact. However, the existing enzymolysis reaction device cannot oscillate and incubate the reactants at a constant temperature, which is not convenient for use in the production of flavonoid glycoside compounds. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing an enzymolysis reaction device to solve the problem that the existing enzymolysis reaction device cannot oscillate and incubate the reactants at a constant temperature, which is not suitable for the production of flavonoid glycoside compounds.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme

[0007] The utility model provides an enzymolysis reaction device, including thermostat, the inside bottom of thermostat is equipped with crank slider assembly, the inside bottom of thermostat is equipped with guide rod in front and back, install the oscillating disc on guide rod, oscillating disc can move left and right on guide rod, the top of crank slider assembly is connected with oscillating disc, a plurality of trays are equipped from top to bottom on oscillating disc, and the detachable connection of tray and oscillating disc is connected.

[0008] Preferably, the side of the thermostat is provided with an electrical connection line, the end of the electrical connection line is provided with an electrical connection plug, and the front bottom of the thermostat is provided with a control panel.

[0009] Preferably, the front opening of the thermostat is connected with a door through a hinge, the middle of the door is provided with an observation window, and the door is provided with a handle; the inside bottom of the thermostat is provided with a supporting bottom plate.

[0010] Preferably, the crank slider assembly includes a mounting plate, a motor, a crank, a connecting rod and a slide rod; the motor is installed at the bottom of the mounting plate, the output end of the motor penetrates through the mounting plate and is connected with the crank, the crank is connected with the slide rod through the connecting rod, the mounting plate is provided with a guide block, and one end of the slide rod penetrates through the middle of the guide block; under the driving of the motor, the slide rod can move back and forth along the guide block.

[0011] Preferably, the bottom of both ends of the mounting plate is provided with a mounting foot, and the mounting foot is provided with a plurality of mounting holes; one end of the slide rod penetrating through the guide block is provided with a connecting plate, and the connecting plate is provided with a plurality of connecting holes.

[0012] Preferably, the side of the motor is provided with a communication line, the end of the communication line is provided with a communication plug, and the communication plug is electrically connected with the thermostat.

[0013] Preferably, the bottom of the oscillating disc is provided with a connecting block, the middle of the connecting block is provided with a linear bearing, and the linear bearing is sleeved on the guide rod.

[0014] Preferably, the both ends of the guide rod are provided with a fixing disc, and the edge of the fixing disc is provided with a plurality of fixing holes.

[0015] Preferably, the both sides of the tray are symmetrically provided with a grip, the inside of the tray is provided with a limiting plate, the limiting plate is provided with a plurality of limiting holes, the bottom of the tray is provided with a bottom plate, and the bottom plate is used for embedding into the inside of the adjacent tray or oscillating disc.

[0016] Preferably, it further includes a gland, the front end face of the oscillating disc is provided with a sleeve joint, the middle of the sleeve joint is provided with a sleeve hole, the position of the sleeve joint aligning with the sleeve hole is provided with a pressing knob, the upper surface of the gland is provided with a handle, the bottom surface of the gland is provided with an insertion plate, the front face of the gland is provided with an insertion rod, the insertion rod is inserted into the inside of the sleeve hole, and the pressing knob is abutted on the insertion rod.

[0017] Compared with the prior art, the utility model has the advantages of:

[0018] 1, the utility model discloses a crank slide bar assembly is installed in the thermostat, and the reaction substrate or the container with substrate is placed in the inside of the tray through the crank slide bar assembly, and the reaction substrate is oscillated back and forth under the action of the oscillation disc, and the enzyme is fully contacted with the substrate, and the oscillation disc is also stable under the action of the guide rod.

[0019] 2, the utility model discloses a gland, and the tray is pressed on the top of the oscillation disc through the gland, and the oscillation disc carries the tray and oscillates, and the reaction substrate is stable on the tray in the oscillation process. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 It is the whole structure schematic diagram of the utility model;

[0021] Figure 2 It is the structure schematic diagram of the thermostat of the utility model;

[0022] Figure 3 It is the structure schematic diagram of the crank slide bar assembly of the utility model;

[0023] Figure 4 It is the structure schematic diagram of the oscillation disc of the utility model;

[0024] Figure 5 It is the structure schematic diagram of the guide rod of the utility model;

[0025] Figure 6 It is the structure schematic diagram of the tray of the utility model;

[0026] Figure 7 It is the structure schematic diagram of the gland of the utility model.

[0027] In the drawing: 1, thermostat;11, power line;12, power plug;13, control panel;14, door;15, observation window;16, handle;17, support bottom plate;18, hinge;2, crank slide bar assembly;21, mounting plate;211, mounting foot;212, mounting hole;213, guide block;22, motor;221, communication line;222, communication plug;23, crank;24, connecting rod;25, slide bar;251, connecting plate;252, connecting hole;3, oscillation disc;31, socket joint;32, socket hole;33, pressing knob;34, connecting block;35, linear bearing;4, guide rod;41, fixed disc;42, fixed hole;5, tray;51, grip;52, limiting plate;53, limiting hole;54, bottom plate;6, gland;61, handle;62, plug plate;63, plug rod. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the utility model will be apparently and completely described in connection with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all the other embodiments obtained by the ordinary skilled in the art without creative labor belong to the protection scope of the utility model.

[0029] Embodiment 1

[0030] Please refer to Figure 1 An enzymolysis reaction device, comprising a thermostat 1, the thermostat 1 is convenient for providing a constant temperature environment for the substrate of enzymolysis reaction. The inside bottom of the thermostat 1 is provided with a crank slide bar assembly 2, the inside bottom of the thermostat 1 is provided with a guide rod 4 at both ends, the guide rod 4 is installed with an oscillating disc 3, the oscillating disc 3 can move left and right on the guide rod 4, and the top of the crank slide bar assembly 2 is connected with the oscillating disc 3; the crank slide bar assembly 2 is used to drive the oscillating disc 3 to move back and forth on the guide rod 4. A plurality of trays 5 are arranged on the oscillating disc 3 from top to bottom, and the tray 5 is detachably connected with the oscillating disc 3; the tray 5 is convenient for placing the substrate of enzymolysis reaction, so as to be convenient for oscillation and stirring under the driving of the oscillating disc 3.

[0031] Please refer to Figure 2 The side of the thermostat 1 is provided with an electric wire 11, and the end of the electric wire 11 is provided with an electric plug 12; the electric wire 11 and the electric plug 12 are convenient for electrifying the thermostat 1 and facilitating the operation of the thermostat 1. The front bottom of the thermostat 1 is provided with a control panel 13, and the control panel 13 is convenient for controlling the opening and closing of the thermostat 1. The front opening of the thermostat 1 is connected with a box door 14 through a hinge 18, and the box door 14 is convenient for opening and closing the thermostat 1. The middle of the box door 14 is provided with an observation window 15, and the box door 14 is provided with a handle 16; the handle 16 is convenient for opening the box door 14, and the observation window 15 is convenient for observing the inside of the thermostat 1. The inside bottom of the thermostat 1 is provided with a supporting bottom plate 17, and the supporting bottom plate 17 is used to install the crank slide bar assembly 2.

[0032] Please refer to Figure 3The crank slider assembly 2 comprises a mounting plate 21, a motor 22, a crank 23, a connecting rod 24 and a slider 25. The motor 22 is mounted at the bottom of the mounting plate 21, and the output end of the motor 22 penetrates through the mounting plate 21 and is connected with the crank 23. The crank 23 is connected with the slider 25 through the connecting rod 24. The mounting plate 21 is provided with a guide block 213, and the slider 25 penetrates through the middle of the guide block 213. Under the driving of the motor 22, the slider 25 can move back and forth along the guide block 213. The bottom of each end of the mounting plate 21 is provided with a mounting foot 211, and the mounting foot 211 is provided with a plurality of mounting holes 212. The mounting foot 211 and the mounting hole 212 are convenient for fixing the position of the mounting plate 21 through bolts. One end of the slider 25 penetrating through the guide block 213 is provided with a connecting plate 251, and the connecting plate 251 is provided with a plurality of connecting holes 252. The connecting plate 251 and the connecting hole 252 are convenient for connecting the slider 25 with the oscillating disc 3 through bolts. The side surface of the motor 22 is provided with a communication line 221, and the end of the communication line 221 is provided with a communication plug 222. The communication plug 222 is electrically connected with the thermostat box 1, which is convenient for controlling and operating the motor 22.

[0033] Please refer to Figure 4 The bottom of the oscillating disc 3 is provided with a connecting block 34, and the middle of the connecting block 34 is provided with a linear bearing 35. The linear bearing 35 is sleeved on the guide rod 4. This structure is convenient for moving the oscillating disc 3 back and forth on the guide rod 4, and is convenient for moving the tray 5 back and forth under the driving of the oscillating disc 3.

[0034] Please refer to Figure 5 The two ends of the guide rod 4 are provided with fixed discs 41, and the edges of the fixed discs 41 are provided with a plurality of fixed holes 42. The fixed disc 41 and the fixed hole 42 are convenient for fixing the position of the guide rod 4 through bolts.

[0035] Please refer to Figure 6 The two sides of the tray 5 are symmetrically provided with holding openings 51, which are convenient for holding and disassembling the tray 5. The inner side of the tray 5 is provided with a limiting plate 52, and the limiting plate 52 is provided with a plurality of limiting holes 53. The limiting plate 52 and the limiting hole 53 are convenient for limiting the containers containing reaction substrates. The bottom of the tray 5 is provided with a bottom plate 54, which is used for embedding into the adjacent tray 5 or the inside of the oscillating disc 3.

[0036] Example 2

[0037] Please refer to Figure 1The utility model provides an enzymolysis reaction device, including thermostat 1, thermostat 1 is convenient for providing a constant temperature environment for the substrate of enzymolysis reaction, the inside bottom of thermostat 1 is equipped with crank slider assembly 2, the inside bottom of thermostat 1 is equipped with guide rod 4 in front and back, is installed with oscillating disc 3 on guide rod 4, oscillating disc 3 can move left and right on guide rod 4, and the top of crank slider assembly 2 is connected with oscillating disc 3, and crank slider assembly 2 is used to drive oscillating disc 3, so that oscillating disc 3 moves back and forth on guide rod 4, a plurality of trays 5 are equipped from top to bottom on oscillating disc 3, and the detachable connection of tray 5 and oscillating disc 3, and tray 5 is convenient for placing the substrate of enzymolysis reaction, to be convenient for oscillating stirring under the drive of oscillating disc 3.

[0038] Please refer to Figure 2 The side of thermostat 1 is equipped with electricity wire 11, and the end of electricity wire 11 is equipped with electricity plug 12, and electricity wire 11 and electricity plug 12 are convenient for the power supply of thermostat 1, and the operation of thermostat 1, and the bottom of the front of thermostat 1 is equipped with control panel 13, and control panel 13 is convenient for controlling the opening and closing of thermostat 1, and the opening of the front of thermostat 1 is connected with box door 14 through hinge 18, and box door 14 is convenient for the opening and closing of thermostat 1, and the middle of box door 14 is equipped with observation window 15, and box door 14 is equipped with handle 16, and handle 16 is convenient for opening box door 14, and observation window 15 is convenient for observing the inside condition of thermostat 1, and the inside bottom of thermostat 1 is equipped with supporting bottom plate 17, and supporting bottom plate 17 is used for installing crank slider assembly 2.

[0039] Please refer to Figure 3 Crank slider assembly 2 includes mounting plate 21, motor 22, crank 23, connecting rod 24 and slide bar 25, motor 22 is installed at the bottom of mounting plate 21, and the output end of motor 22 passes through mounting plate 21 and is connected with crank 23, crank 23 is connected with slide bar 25 through connecting rod 24, and mounting plate 21 is equipped with guide block 213, and slide bar 25 passes through the middle of guide block 213, and under the drive of motor 22, slide bar 25 can stretch and contract along guide block 213, and the both ends of bottom of mounting plate 21 are equipped with mounting feet 211, and mounting feet 211 are equipped with a plurality of mounting holes 212, and mounting feet 211 and mounting holes 212 are convenient for fixing the position of mounting plate 21 through bolt, and one end of slide bar 25 passing through guide block 213 is equipped with connecting plate 251, and connecting plate 251 is equipped with a plurality of connecting holes 252, and the cooperation of connecting plate 251 and connecting hole 252 is convenient for connecting slide bar 25 with oscillating disc 3 through bolt, and the side of motor 22 is equipped with intercommunication wire 221, and the end of intercommunication wire 221 is equipped with intercommunication plug 222, and intercommunication plug 222 is electrically connected with thermostat 1, and it is convenient for the control of motor 22 and the operation of motor 22.

[0040] Please refer to Figure 4, the bottom of the oscillating disc 3 is provided with a connecting block 34, the middle of the connecting block 34 is provided with a linear bearing 35, and the linear bearing 35 is sleeved on the guide rod 4; this structure is convenient for the oscillating disc 3 to move back and forth on the guide rod 4, and is convenient for the oscillating disc 3 to drive the tray 5 to move back and forth.

[0041] Please refer to Figure 5 , the two ends of the guide rod 4 are provided with fixed discs 41, and the edges of the fixed discs 41 are provided with a plurality of fixed holes 42; the cooperation of the fixed disc 41 and the fixed hole 42 is convenient for fixing the position of the guide rod 4 through bolts.

[0042] Please refer to Figure 6 , the two sides of the tray 5 are symmetrically provided with holding openings 51, the holding openings 51 are convenient for holding the tray 5, and the tray 5 is convenient for disassembly and assembly. The inner side of the tray 5 is provided with a limiting plate 52, the limiting plate 52 is provided with a plurality of limiting holes 53, and the cooperation of the limiting plate 52 and the limiting hole 53 is convenient for limiting the container containing the reaction substrate. The bottom of the tray 5 is provided with a bottom plate 54, which is used for embedding the adjacent tray 5 or the inside of the oscillating disc 3.

[0043] Please refer to Figure 1 、 Figure 4 and Figure 7 , the pressure cover 6 is convenient for pressing and limiting the tray 5, and is also convenient for stabilizing the reaction in the tray 5. The front end face of the oscillating disc 3 is provided with a sleeve joint 31, the middle of the sleeve joint 31 is provided with a sleeve hole 32, and the position of the sleeve joint 31 aligning the sleeve hole 32 is provided with a pressing knob 33; the pressing knob 33 is convenient for fixing the pressure cover 6, the upper surface of the pressure cover 6 is provided with a handle 61, the handle 61 is convenient for disassembly and assembly of the pressure cover 6. The bottom surface of the pressure cover 6 is provided with an insertion plate 62, which is convenient for inserting into the inner side of the tray 5. The front surface of the pressure cover 6 is provided with an insertion rod 63, which is inserted into the inner side of the sleeve hole 32, and the pressing knob 33 abuts against the insertion rod 63, which can ensure that the pressure cover 6 is stable enough.

[0044] Working principle: in use, according to the use requirement, the reaction required by enzymatic reaction is injected into the container, and the container is placed in the inner side of the tray 5, then the tray 5 is placed on the oscillating disc 3, and the position of the tray 5 is fixed by the pressure cover 6, so that the tray 5 is stable on the oscillating disc 3. Then control the incubator 1 to heat, so that the temperature in the incubator 1 rises to a specified temperature, and start the crank slide assembly 2, so that the crank slide assembly 2 drives the oscillating disc 3 to move back and forth, so as to achieve the purpose of sufficient contact between the enzyme and the substrate.

[0045] Compared with the prior art, the application is characterized in that a crank slide assembly 2 is installed in the thermostat 1, the oscillating disc 3 can be driven to swing left and right by the crank slide assembly 2, a plurality of trays 5 can be installed on the top of the oscillating disc 3, the substrates to be reacted or the containers containing the substrates can be loaded in the trays 5, the reaction substances in the trays 5 can be oscillated back and forth under the action of the oscillating disc 3, the contact between the enzymes and the substrates is ensured, and the oscillating disc 3 is also stable enough under the action of the guide rod 4.

[0046] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. An enzymatic reaction device comprising an incubator (1), characterized in that: The inside bottom of the thermostat (1) is provided with a crank sliding rod assembly (2), the inside bottom of the thermostat (1) is provided with a guide rod (4) at the front and rear ends, a vibrating disc (3) is installed on the guide rod (4), the vibrating disc (3) can move left and right on the guide rod (4), and the vibrating disc (3) is connected with the crank sliding rod assembly (2) at the top.

2. The enzymatic reaction device according to claim 1, wherein: The side of the thermostat (1) is provided with an electric wire (11), the end of the electric wire (11) is provided with an electric plug (12), and the front bottom of the thermostat (1) is provided with a control panel (13).

3. The enzymatic reaction device according to claim 2, wherein: The front opening of the thermostat (1) is connected with a box door (14) through a hinge (18), the middle of the box door (14) is provided with an observation window (15), and the box door (14) is provided with a handle (16); and the inside bottom of the thermostat (1) is provided with a supporting bottom plate (17).

4. The enzymatic reaction device according to claim 1, wherein: The crank sliding rod assembly (2) comprises a mounting plate (21), a motor (22), a crank (23), a connecting rod (24) and a sliding rod (25); the motor (22) is installed at the bottom of the mounting plate (21), the output end of the motor (22) penetrates through the mounting plate (21) and is connected with the crank (23), the crank (23) is connected with the sliding rod (25) through the connecting rod (24), and the mounting plate (21) is provided with a guide block (213); and the sliding rod (25) penetrates through the middle of the guide block (213), and under the driving of the motor (22), the sliding rod (25) can move back and forth along the guide block (213).

5. The enzymatic reaction device according to claim 4, wherein: The bottom of the mounting plate (21) is provided with a mounting foot (211), and a plurality of mounting holes (212) are formed in the mounting foot (211); one end of the sliding rod (25) penetrating through the guide block (213) is provided with a connecting plate (251), and a plurality of connecting holes (252) are formed in the connecting plate (251).

6. The enzymatic reaction device according to claim 5, wherein: The side of the motor (22) is provided with a communication line (221), and the end of the communication line (221) is provided with a communication plug (222), and the communication plug (222) is electrically connected with the thermostat (1).

7. The enzymatic reaction device of claim 1, wherein: The bottom of the vibrating disc (3) is provided with a connecting block (34), the middle of the connecting block (34) is provided with a linear bearing (35), and the linear bearing (35) is sleeved on the guide rod (4).

8. The enzymatic reaction device according to claim 7, wherein: The two ends of the guide rod (4) are provided with a fixed disc (41), and a plurality of fixed holes (42) are formed in the edge of the fixed disc (41).

9. The enzymatic reaction device of claim 1, wherein: The two sides of the tray (5) are symmetrically provided with a holding opening (51), the inside of the tray (5) is provided with a limiting plate (52), a plurality of limiting holes (53) are formed in the limiting plate (52), the bottom of the tray (5) is provided with a bottom plate (54), and the bottom plate (54) is used for embedding into the inside of the adjacent tray (5) or vibrating disc (3).

10. The enzymatic reaction device according to any one of claims 1 to 9, characterized in that: Also include the gland (6), the oscillating disc (3) front end surface is equipped with the sleeve joint (31), the sleeve joint (31) middle part is equipped with sleeve hole (32), and the sleeve joint (31) alignment sleeve hole (32) position is equipped with the compression knob (33);The upper surface of the gland (6) is equipped with a handle (61), the bottom surface of the gland (6) is equipped with an insertion plate (62), the front surface of the gland (6) is equipped with an insertion rod (63), the insertion rod (63) is inserted into the inside of the sleeve hole (32), and the compression knob (33) is against the insertion rod (63).

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