Mouse embryo culture device
The embryo culture device, designed with a motor-driven rotary table and convex-concave slide rails, overcomes the limitations of existing devices in simulating physiological environments, achieves stable embryo development and simplifies operation, reduces costs, and ensures the stability of a sterile environment.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-15
- Publication Date
- 2026-03-27
AI Technical Summary
Existing mouse embryo culture devices have limitations in simulating the complex physiological environment within the maternal uterus. They fail to fully reproduce dynamic fluid flow and appropriate mechanical stimulation, leading to abnormal embryo development. Furthermore, their operational complexity and high cost limit their widespread application.
A mouse embryo culture device was designed. The rotating table is driven by a motor to rotate slowly. Combined with the concave and convex slide rail design, the culture dish is intermittently shaken to simulate the mechanical stimulation and flow conditions of the in vivo environment. Locking components are used to ensure the stability and sealing of the culture dish and prevent external contamination.
It improved the quality of embryo development, simplified the operation process, reduced costs, and ensured the stability of the sterile environment and the accuracy of experimental results.
Smart Images
Figure CN224047413U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to mouse embryo culture technical field especially, relate to a mouse embryo culture device. BACKGROUND
[0002] Mouse is one of the most commonly used model organisms in biological research, and its embryo culture plays a crucial role in basic scientific research, genetics research, developmental biology, pharmacology testing and assisted reproductive technology. In order to support research in these fields, scientists need to be able to simulate in-vivo environment under laboratory conditions to ensure that embryos can develop normally, so it is particularly important to design a mouse embryo culture device that can provide a stable and controllable environment.
[0003] However, the current culture device has limitations in simulating the complex physiological environment of the maternal uterus, and fails to fully reproduce dynamic liquid flow and appropriate mechanical stimulation, which are essential for maintaining normal embryonic development. The lack of such simulation may lead to abnormal embryonic development, and the complexity of operation and high cost also limit the wider application and development of this technology. SUMMARY
[0004] In order to overcome the shortcomings of the above-mentioned prior art, the utility model provides a mouse embryo culture device.
[0005] The utility model provides a mouse embryo culture device, including box and door board, the box is the assembly carrier of culture device, the opening of putting culture dish is set up on the front side of box, the door board is hinged in the opening of box front side, the outside wall of door board's front side installs the handle, still include: motor, fixed mounting is installed on the inside bottom plate of box, the output shaft of motor is fixed with the male rod, the bottom surface of rotary table is fixedly connected with the hollow sleeve rod, the side wall of sleeve rod is opened the sliding slot, the rotary table is arranged on the output shaft of motor through sleeve rod, the male rod on the output shaft of motor is located in the sliding slot of sleeve rod, and the first spring is arranged between the output shaft of motor and rotary table, the limiting plate is fixedly arranged at the four corners of rotary table top surface, and the limiting plate is used for limiting culture dish placed on the rotary table, the concave-convex slide rail is fixedly arranged on the inside bottom plate of box, the concave-convex slide rail is arranged on the circumference of motor, and a circle of symmetrical concave-convex slide rail is arranged on the concave-convex slide rail, the contact rod is arranged with two and is symmetrically fixedly arranged on the bottom surface of rotary table, the lower end of contact rod and the concave-convex slide rail on the concave-convex slide rail sliding contact, the locking piece is arranged on the rotary table, and the locking piece is used for locking culture dish placed on the rotary table, improves the stability of culture dish placed on the rotary table, utilizes motor to drive rotary table to rotate slowly, and rotary table is slid along the concave-convex slide rail on the concave-convex slide rail through contact rod, cooperates the elastic effect of rotary table of first spring, makes the culture dish stably placed on rotary table to occur intermittent shaking while rotating, thereby creates mouse embryo under the condition of proper mechanical stimulation and flowing culture solution, thereby simulates the environment more close to mouse embryo in natural physiological state, is favorable to maintaining and promoting the normal development of mouse embryo.
[0006] As preferred, the locking piece includes a clamping plate, a wedge-shaped block and a support plate, the clamping plate is symmetrically fixedly connected to one side of the outer wall of the culture dish, the clamping plate is provided with a clamping groove, the support plate is symmetrically fixedly arranged on one side of the top surface of the rotary table, the number of the wedge-shaped block is consistent with the clamping plate, and the wedge-shaped block is slidably arranged on the corresponding support plate through a guide rod, the second spring is arranged between the wedge-shaped block and the support plate, the wedge-shaped block is adapted and clamped in the clamping groove of the corresponding clamping plate, and the culture dish is limited on the rotary table by the limiting plate. Then, the wedge-shaped block is clamped into the clamping plate of the rotary table, so that the culture dish can be stably placed on the rotary table for culture.
[0007] As preferred, a double-acting pneumatic cylinder is fixedly installed on one side of the top surface of the rotary table close to the wedge-shaped block, the double-acting pneumatic cylinder is provided with a pressing plate on the double-acting telescopic rod, the pressing plate is in contact with the corresponding wedge-shaped block on the same side, the double-acting pneumatic cylinder presses the corresponding wedge-shaped block through the pressing plate, the wedge-shaped block compresses the second spring and automatically decouples with the corresponding clamping plate, after the pressing plate of the double-acting pneumatic cylinder no longer presses the wedge-shaped block, the wedge-shaped block is reset and clamped into the clamping plate under the action of the second spring, so that the culture dish can be conveniently locked and unlocked on the rotary table.
[0008] As preferred, the box is symmetrically fixed with a fixed shaft near the two sides of the outer wall of the front opening, the rotating plate is rotatably installed on the fixed shaft, the clamping block is symmetrically fixed on the outer wall of the door plate near the rotating plate, the clamping block is an L-shaped block and is matched with the rotating plate on the same side, and the rotating plate can be clamped into the clamping block on the same side, so as to lock the door plate on the box.
[0009] As preferred, the rotating plate is provided with a clamping hole at one end away from the clamping block, the spring telescopic rod is symmetrically arranged on the front outer wall of the box near the door plate, and the spring telescopic rod is matched with the clamping hole on the rotating plate. After the rotating plate rotates by a certain angle and contacts the clamping buckle of the clamping block, the rotating plate is elastically clamped into the corresponding spring telescopic rod through the clamping hole on the rotating plate, so that the rotating plate is stably kept and the clamping block is released.
[0010] As preferred, the inner wall side of the door plate is provided with a sealing gasket, the sealing gasket is consistent with the opening area of the front side of the box, and the sealing gasket can tightly block the opening of the front side of the box, so that the inside and outside of the box are in a relatively sealed and isolated state, thereby avoiding the adverse effects of the external environment on the mouse embryos in the culture dish in the box.
[0011] Beneficial effects are: 1. The utility model discloses a motor-driven rotating table slowly rotates, and combines the design of the concave-convex slide rail, so that the culture dish on the rotating table is intermittently shaken, thereby creating mechanical stimulation and flow conditions similar to the in-vivo environment, which helps to improve the development quality of embryos.
[0012] 2. The utility model discloses a locking piece to ensure the stability of the culture dish placed on the rotating table, prevent it from shifting or falling off during rotation, and through the cooperation of the rotating plate, the sealing gasket and the clamping block, ensure the airtightness and sterile environment of the whole system, and avoid the influence of external pollution on the experimental results.
[0013] 3. The utility model discloses a bidirectional air cylinder matched with the extrusion plate can easily realize the automatic release and reset of the wedge-shaped block, simplify the installation and unloading process of the culture dish. ACCURACY
[0014] Figure 1 It is a three-dimensional structure schematic diagram of the utility model.
[0015] Figure 2 It is a position relation diagram of the box, the rotating table, the motor and the culture dish of the utility model.
[0016] Figure 3 It is a connection relation diagram of the box, the motor, the convex rod and the concave-convex slide rail of the utility model.
[0017] Figure 4 It is an explosion diagram of the motor and the rotating table of the utility model.
[0018] Figure 5 This is a schematic diagram of the rotary table, clamping plate, wedge block, support plate and double-acting cylinder of this utility model.
[0019] Figure 6 This is a schematic diagram of the door panel and sealing gasket of this utility model.
[0020] Explanation of reference numerals in the attached drawings: 1-box body, 2-door panel, 3-handle, 4-motor, 41-protruding rod, 5-rotating table, 51-sleeve rod, 52-limiting plate, 6-first spring, 7-concave-convex slide rail, 8-contact rod, 81-culture dish, 9-fixed shaft, 10-rotating plate, 101-locking hole, 11-locking block, 12-spring telescopic rod, 13-locking plate, 14-wedge block, 15-support plate, 16-second spring, 17-double-acting cylinder, 171-pressing plate, 18-sealing gasket. Detailed Implementation
[0021] Exemplary embodiments will now be described in detail, examples of which are illustrated in the accompanying drawings. When the following description refers to the drawings, unless otherwise indicated, the same numbers in different drawings denote the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with this application. Rather, they are merely examples of apparatuses and methods consistent with some aspects of this application as detailed in the appended claims.
[0022] A mouse embryo culture device, such as Figures 1-5As shown, including the box 1 and door plate 2, box 1 is the assembly carrier of the present culture device, the front side of the box 1 is provided with an opening for placing the culture dish 81, the door plate 2 is hinged to the opening of the front side of the box 1, the front side of the door plate 2 is provided with a handle 3; further comprising: motor 4, fixedly installed on the inner bottom plate of the box 1, the output shaft of the motor 4 is provided with a convex rod 41; rotating table 5, the bottom surface of the rotating table 5 is fixedly connected with a hollow sleeve rod 51, the side wall of the sleeve rod 51 is provided with a sliding groove, the rotating table 5 is arranged on the output shaft of the motor 4 through the sleeve rod 51, the convex rod 41 on the output shaft of the motor 4 is located in the sliding groove of the sleeve rod 51, the first spring 6 is arranged between the output shaft of the motor 4 and the rotating table 5; limiting plate 52, fixedly arranged at the four corners of the top surface of the rotating table 5, the limiting plate 52 is used for limiting the culture dish 81 placed on the rotating table 5; concave-convex slide rail 7, fixedly arranged on the inner bottom plate of the box 1, the concave-convex slide rail 7 is arranged around the circumference of the motor 4, and a circle of symmetrical concave-convex slide rails is arranged on the concave-convex slide rail 7; contact rod 8, two contact rods 8 are symmetrically arranged on the bottom surface of the rotating table 5, the lower end of the contact rod 8 is in sliding contact with the concave-convex slide rail 7; locking member, arranged on the rotating table 5, the locking member is used for locking the culture dish 81 placed on the rotating table 5, improving the stability of the culture dish 81 placed on the rotating table 5, driving the rotating table 5 to rotate slowly by the motor 4, the rotating table 5 slides along the concave-convex slide rail 7 through the contact rod 8, cooperating with the elastic effect of the first spring 6 on the rotating table 5, so that the culture dish 81 stably placed on the rotating table 5 is intermittently shaken while rotating, thereby creating a condition of appropriate mechanical stimulation and flowing culture solution for mouse embryos, thereby simulating an environment closer to the natural physiological state of mouse embryos, which is conducive to maintaining and promoting the normal development of mouse embryos.
[0023] As shown in Figure 2 and Figure 5 The locking member includes a clamping plate 13, a wedge block 14 and a support plate 15, the clamping plate 13 is symmetrically fixedly connected to one side of the outer wall of the culture dish 81, the clamping plate 13 is provided with a clamping groove, the support plate 15 is symmetrically fixedly arranged on one side of the top surface of the rotating table 5, the number of the wedge block 14 is consistent with the clamping plate 13 and is slidably arranged on the corresponding support plate 15 through a guide rod, the second spring 16 is arranged between the wedge block 14 and the support plate 15, the wedge block 14 is adapted and clamped in the clamping groove of the corresponding clamping plate 13, the culture dish 81 is limited on the rotating table 5 by the limiting plate 52, and then the wedge block 14 is clamped into the clamping plate 13 of the rotating table 5, so that the culture dish 81 can be stably placed on the rotating table 5 for culture.
[0024] As shown in Figure 2 and Figure 5As shown, the top surface of the rotating table 5 near one side of the wedge block 14 is fixedly provided with a bidirectional air cylinder 17, and the bidirectional telescopic rod of the bidirectional air cylinder 17 is fixedly provided with an extrusion plate 171. The extrusion plate 171 is in contact with the corresponding wedge block 14 on the same side. The bidirectional air cylinder 17 extrudes the corresponding wedge block 14 through the extrusion plate 171, so that the wedge block 14 compresses the second spring 16 and automatically releases the corresponding clamping plate 13. After the extrusion plate 171 of the bidirectional air cylinder 17 no longer extrudes the wedge block 14, the wedge block 14 is reset under the action of the second spring 16 and clamped into the clamping plate 13, so that the culture dish 81 can be conveniently locked and unlocked on the rotating table 5.
[0025] As shown in Figure 1 , the box body 1 is symmetrically and fixedly provided with a fixed shaft 9 on both sides of the outer wall near the front opening. The fixed shaft 9 is rotatably provided with a rotating plate 10. The outer wall of the door plate 2 is symmetrically and fixedly provided with a clamping block 11 near the rotating plate 10. The clamping block 11 is an “L” shaped block and is adapted to the rotating plate 10 on the same side. The rotating plate 10 can be clamped into the clamping block 11 on the same side, so as to lock and close the door plate 2 on the box body 1. The end of the rotating plate 10 away from the clamping block 11 is provided with a clamping hole 101. The box body 1 is symmetrically provided with a spring telescopic rod 12 near the door plate 2 on the front outer wall. The spring telescopic rod 12 is adapted to the clamping hole 101 on the rotating plate 10. After the rotating plate 10 is rotated by a certain angle and contacts the clamping block 11, the rotating plate 10 is elastically clamped into the corresponding spring telescopic rod 12 through the clamping hole 101 thereon, so as to stably keep the state of being released from the clamping block 11.
[0026] As shown in Figure 1 , Figure 2 , and Figure 6 , the inner wall of the door plate 2 is provided with a sealing gasket 18. The sealing gasket 18 is consistent with the opening area of the front side of the box body 1. The sealing gasket 18 can tightly block the opening on the front side of the box body 1, so that the inside and outside of the box body 1 are in a relatively sealed and isolated state, avoiding the adverse effects of the external environment on the mouse embryos in the culture dish 81 in the box body 1.
[0027] When the culture device is used to culture mouse embryos, the operator unlocks the door plate 2 by disengaging the rotating plate 10 from the L-shaped clamping block 11, and rotating the disengaged rotating plate 10 is elastically stabilized by the clamping hole 101 on the rotating plate 10 and clamped into the corresponding spring telescopic rod 12, then the operator opens the door plate 2 by using the handle 3 arranged on the door plate 2, and then places the culture dish 81 containing mouse embryos on the rotating table 5 and ensures that the culture dish 81 is located between the limiting plates 52 to keep stable, at this time the clamping plate 13 on the culture dish 81 will be aligned with the wedge-shaped block 14, then the bidirectional air cylinder 17 is started, the bidirectional telescopic rod of the bidirectional air cylinder 17 drives the corresponding extrusion plate 171 to disengage the extrusion of the wedge-shaped block 14, the wedge-shaped block 14 is popped out outward under the elastic force of the second spring 16 and clamped into the clamping groove on the clamping plate 13, and the limiting plate 52 is limited downward, so as to realize the stable locking of the culture dish 81, then the door plate 2 is closed, and the door plate 2 is locked by cooperating the rotating plate 10 with the clamping block 11, which ensures that the door plate 2 is firmly closed and the sealing gasket 18 tightly blocks the front opening of the box body 1, maintains the stability of the culture environment in the box body 1, finally the motor 4 is started, the motor 4 drives the rotating table 5 to rotate slowly, the contact rod 8 at the bottom of the rotating table 5 moves along the concave-convex sliding rail 7, and the rotating table 5 and the culture dish 81 above it are intermittently shaken due to the action of the first spring 6 between the output shaft of the motor 4 and the rotating table 5, so as to simulate the mechanical stimulation and flow conditions of the mouse embryos in the natural physiological state, which is beneficial to the normal development of the embryos.
[0028] Although the present application is described in detail with reference to the above embodiments, it is obvious to those skilled in the art that various changes or modifications can be made to the present application without departing from the principles and spirit of the application defined in the claims. Therefore, the detailed description of the present application is only used to explain, not to limit the present application, and the protection scope is defined by the content of the claims.
Claims
1. A mouse embryo culture device, comprising a box (1) and a door plate (2), an opening for placing a culture dish (81) is formed on the front side of the box (1), the door plate (2) is hinged to the opening on the front side of the box (1), and a handle (3) is installed on the front side outer wall of the door plate (2). characterized in that further Comprise: a motor (4) fixedly installed on the inner bottom plate of the box (1), a protruding rod (41) is fixedly arranged on the output shaft of the motor (4); a rotating table (5), a hollow sleeve rod (51) is fixedly connected to the bottom surface of the rotating table (5), a sliding groove is formed in the side wall of the sleeve rod (51), the rotating table (5) is arranged on the output shaft of the motor (4) through the sleeve rod (51), the protruding rod (41) on the output shaft of the motor (4) is located in the sliding groove of the sleeve rod (51), and a first spring (6) is arranged between the output shaft of the motor (4) and the rotating table (5); a limiting plate (52) fixedly arranged at the four corners of the top surface of the rotating table (5), the limiting plate (52) is used for limiting the culture dish (81) placed on the rotating table (5); a concave-convex sliding rail (7) fixedly arranged on the inner bottom plate of the box (1), the concave-convex sliding rail (7) is arranged around the motor (4), and a circle of symmetrical concave-convex sliding rails is arranged on the concave-convex sliding rail (7); two contact rods (8) symmetrically fixedly arranged on the bottom surface of the rotating table (5), and the lower end of the contact rod (8) is in sliding contact with the concave-convex sliding rail (7); a locking member arranged on the rotating table (5) and used for locking the culture dish (81) placed on the rotating table (5).
2. The mouse embryo culture device of claim 1, wherein The locking member comprises a clamping plate (13), a wedge-shaped block (14) and a supporting plate (15), the clamping plate (13) is symmetrically fixedly connected to one side of the outer wall of the culture dish (81), a clamping groove is formed in the clamping plate (13), the supporting plate (15) is symmetrically fixedly arranged on one side of the top surface of the rotating table (5), the number of the wedge-shaped blocks (14) is consistent with that of the clamping plates (13), and the wedge-shaped blocks (14) are slidingly arranged on the corresponding supporting plates (15) through guide rods, the second spring (16) is arranged between the wedge-shaped block (14) and the supporting plate (15), and the wedge-shaped block (14) is adapted and clamped in the clamping groove of the corresponding clamping plate (13).
3. The mouse embryo culture device of claim 2, wherein the mouse embryo culture device is a mouse embryo culture dish. A bidirectional air cylinder (17) is fixedly installed on one side of the top surface of the rotating table (5) close to the wedge-shaped block (14), the bidirectional telescopic rod of the bidirectional air cylinder (17) is fixedly provided with a pressing plate (171), the pressing plate (171) is in contact with the corresponding wedge-shaped block (14) on the same side, the bidirectional air cylinder (17) presses the corresponding wedge-shaped block (14) through the pressing plate (171), the wedge-shaped block (14) compresses the second spring (16) and automatically and the corresponding clamping plate (13) is tripped.
4. The mouse embryo culture device of claim 3, wherein the mouse embryo culture device is a mouse embryo culture dish. Fixed shafts (9) are symmetrically fixedly arranged on the two sides of the outer wall of the box (1) close to the front opening, rotating plates (10) are rotatably installed on the fixed shafts (9), and clamping blocks (11) are symmetrically fixedly arranged on the outer wall of the door plate (2) close to the rotating plates (10).
5. The mouse embryo culture device of claim 4, wherein the mouse embryo culture device is a mouse embryo culture dish. The rotating plate (10) is provided with a clamping hole (101) at one end away from the clamping block (11), spring telescopic rods (12) are symmetrically arranged on the outer wall of the front side of the box (1) close to the door plate (2), and the spring telescopic rods (12) are matched with the clamping holes (101) on the rotating plate (10).
6. The mouse embryo culture device of claim 5, wherein the mouse embryo culture device is a mouse embryo culture dish. A sealing gasket (18) is arranged on the inner wall side of the door plate (2), the opening area of the sealing gasket (18) is consistent with that of the front side of the box (1), and the sealing gasket (18) can tightly shield the opening on the front side of the box (1).