Cell immunofluorescence detection antibody incubation device based on laser confocal culture dish

Abstract

The utility model relates to a cell immunofluorescence detection antibody incubation device based on a laser confocal culture dish, which comprises the laser confocal culture dish, the inner bottom surface of the laser confocal culture dish is covered with a detachable incubation cover, the bottom surface of the incubation cover is provided with an incubation groove, and the incubation groove is communicated with the incubation cover. A closed incubation space is defined by the incubation groove and the inner bottom surface of the laser confocal culture dish. Antibody incubation is carried out in the incubation space, so that the antibody dosage can be reduced, and the cost is reduced; the concentration gradient formation caused by antibody evaporation can be prevented, and the experiment repeatability is improved; the design of a cover on the top realizes that the antibody solution uniformly covers the sample, and the dyeing consistency is improved; operation is simple, and errors caused by manual intervention are avoided; due to the detachable design of the incubation cover, the incubation cover can be repeatedly used, and the incubation cover is suitable for cell immunofluorescence, in-situ hybridization and other experimental scenes needing high-precision antibody incubation of laser confocal culture dishes, and is particularly suitable for experiments of precious antibodies.

Description

TECHNICAL FIELD

[0001] The utility model relates to a cell immunofluorescence detection antibody incubation device based on laser confocal culture dish. BACKGROUND

[0002] In the cell immunofluorescence experiment, the following technical defects exist in the antibody incubation link, and the specific problems can be evidenced by the following existing technical patent cases:

[0003] 1) Serious waste of antibodies:

[0004] Traditional open incubation box such as CN 221822224 U needs to cover excess antibody solution (200-300ul / sample), and the reagent is wasted due to the inability to accurately control the liquid distribution. Patent CN 118707090 A points out that the design of large-volume incubation cavity needs to rely on gravity diffusion, and the amount of antibody is forced to increase to cover the sample edge.

[0005] 2) Antibodies are easy to evaporate and have uneven concentration:

[0006] Evaporation problem: the existing device has poor sealing, and long-time incubation (> 30 minutes) leads to evaporation of the antibody solution, significantly changing the concentration. The humidity of the open culture plate in CN 221822224 U is uncontrollable, and the evaporation rate is as high as 15% / hour, while the antibody incubation of traditional cell immunofluorescence often needs 14 hours or even longer.

[0007] Concentration gradient formation: Patent CN 116626279 A mentions that evaporation is concentrated on the edge of the solution during static incubation, forming a center-edge concentration difference (experiments show that the edge fluorescence signal intensity decreases by 20%-30%).

[0008] 3) Complicated operation and pollution risk:

[0009] CN 221822224 U shows that traditional culture plates need to be frequently opened to take and place glass slides, which is easy to introduce external pollutants or cause solution evaporation interference.

[0010] In order to solve the above technical problems, the cell immunofluorescence detection antibody incubation device based on laser confocal culture dish is proposed. Utility model content

[0011] In view of the deficiencies of the prior art, the technical problem to be solved by the utility model is to provide a cell immunofluorescence detection antibody incubation device based on laser confocal culture dish.

[0012] In order to solve the above technical problems, the technical scheme of the utility model is: a cell immunofluorescence detection antibody incubation device based on a laser confocal culture dish, which comprises a laser confocal culture dish, a detachable incubation cover is arranged on the inner bottom surface of the laser confocal culture dish, an incubation groove is arranged on the bottom surface of the incubation cover, and the incubation groove and the inner bottom surface of the laser confocal culture dish jointly form a closed incubation space.

[0013] Preferably, the incubation groove is concave upwards along the bottom surface of the incubation cover.

[0014] Preferably, the bottom surface of the incubation cover is a smooth surface used for being pressed and attached to the inner bottom surface of the laser confocal culture dish.

[0015] Preferably, an elastic member is connected to the incubation cover, and the elastic member is used to provide the pressure of the incubation cover arranged on the inner bottom surface of the laser confocal culture dish.

[0016] Preferably, the elastic member comprises an elastic sheet, the head end of the elastic sheet is fixed to the outer top surface of the incubation cover, and the tail end of the elastic sheet is connected to a clamping assembly.

[0017] Preferably, the clamping assembly comprises a buckle fixed to the tail end of the elastic sheet.

[0018] Preferably, a clamping slot is arranged on the buckle, and the clamping slot is arranged downward.

[0019] Preferably, the width of the clamping slot is greater than or equal to the thickness of the sidewall of the laser confocal culture dish.

[0020] Preferably, the shape of the incubation cover is cylindrical.

[0021] Preferably, the shape of the incubation groove is circular.

[0022] Compared with the prior art, the cell immunofluorescence detection antibody incubation device based on the laser confocal culture dish has the following beneficial effects: the antibody incubation is carried out in the incubation space, the use amount of the antibody can be reduced, and the cost can be reduced; the concentration gradient caused by the evaporation of the antibody can be prevented, and the experimental repeatability can be improved; the design of the top cover (the incubation cover) can realize the uniform covering of the antibody solution on the sample, and the dyeing consistency can be improved; the operation is simple, and the error caused by manual intervention can be avoided; the detachable design of the incubation cover ensures the reusability, and the cell immunofluorescence, in situ hybridization and other experiments requiring high-precision antibody incubation of the laser confocal culture dish can be carried out, and the device is especially suitable for the experiments of precious antibodies.

[0023] The utility model will be explained in further detail in combination with the drawings and specific embodiments. BRIEF DESCRIPTION OF DRAWINGS

[0024] Fig. 1 It is a structure schematic view of the embodiment of the utility model.

[0025] Fig. 2 It is a structure schematic view of the incubation cover.

[0026] Fig. 3 It is a structure schematic view of the laser confocal culture dish. DETAILED DESCRIPTION

[0027] The utility model will be further explained in connection with the drawings and examples.

[0028] It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the present application. Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

[0029] It should be noted that the terms used herein are only for the purpose of describing the specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, the singular form is intended to include the plural form unless the context clearly indicates otherwise, and it should also be understood that when the terms "comprise" and / or "include" are used in the specification, there is a presence of a feature, step, operation, device, component and / or combination thereof.

[0030] As shown in Figs. 1-3 The embodiment provides a cell immunofluorescence detection antibody incubation device based on a laser confocal culture dish, which comprises the laser confocal culture dish 1, the inner bottom surface cover of the laser confocal culture dish is provided with a detachable incubation cover 2, the bottom surface of the incubation cover is provided with an incubation groove 3, and the incubation groove and the inner bottom surface of the laser confocal culture dish jointly form a closed incubation space.

[0031] In the embodiment of the utility model, the incubation groove is concave upwards along the bottom surface of the incubation cover by a certain depth, direct contact with cells is avoided, and meanwhile, the incubation space formed by the smaller incubation groove can save antibodies.

[0032] In the embodiment of the utility model, the bottom surface of the incubation cover is a smooth surface used for being pressed and matched with the inner bottom surface of the laser confocal culture dish.

[0033] In the embodiment of the utility model, the incubation cover is connected with an elastic member, and the elastic member is used for providing the pressure of the incubation cover on the inner bottom surface of the laser confocal culture dish.

[0034] In the embodiment of the utility model, the elastic member comprises an elastic sheet 4, the head end of the elastic sheet is fixed in the middle part of the outer top surface of the incubation cover, and the tail end of the elastic sheet is connected with a clamping assembly.

[0035] In the embodiment of the utility model, the clamping assembly includes the buckle 5 fixed on the tail end of the elastic sheet.

[0036] In the embodiment of the utility model, the buckle is provided with a clamping slot 6, and the clamping slot is provided downward.

[0037] In the embodiment of the utility model, the width of the clamping slot is slightly greater than or equal to the thickness of the sidewall of the laser confocal culture dish.

[0038] In the embodiment of the utility model, the shape of the incubation cover is cylindrical.

[0039] In the embodiment of the utility model, the shape of the incubation groove is circular.

[0040] In the embodiment of the utility model, the working principle of the antibody incubation device based on the laser confocal culture dish is as follows:

[0041] In the initial state, the elastic sheet is not deformed, the incubation cover does not contact the inner bottom surface of the laser confocal culture dish, and the distance between the incubation cover and the buckle is H1.

[0042] When in use:

[0043] S1, add antibodies: use a micropipette to add 80 μL of antibody solution along the central bottom of the dish.

[0044] S2, device installation: the clamping slot of the buckle is directly inserted and installed and fixed on the sidewall of the laser confocal culture dish.

[0045] At the same time, the incubation cover is covered on the inner bottom surface of the laser confocal culture dish, and the antibody solution is contained in the incubation space. When the incubation cover is covered on the inner bottom surface of the laser confocal culture dish, the elastic sheet is bent and deformed, and provides downward pressure, so that the incubation cover is tightly covered on the inner bottom surface of the laser confocal culture dish.

[0046] At this time, the distance between the incubation cover and the buckle is H2, and H2 < H1.

[0047] S3, result detection: after reaching the incubation time (according to experimental requirements), the device is directly disassembled, and subsequent steps can be performed, and the device can be recycled and washed for reuse.

[0048] The above merely describes preferred embodiments of the present application, and is not intended to limit the present application in other forms, and any skilled person in the art can modify or change the above disclosed technical content to equivalent embodiments with equivalent changes. However, any simple modification, equivalent change and modification made to the above embodiments without departing from the technical solution of the present application, and in accordance with the technical essence of the present application, still belong to the protection scope of the technical solution of the present application.

Claims

1. A cell immunofluorescence detection antibody incubation device based on a laser confocal culture dish, comprising a laser confocal culture dish, characterized in that: The inner bottom surface of the laser confocal culture dish is covered with a removable incubation cover, and the bottom surface of the incubation cover is provided with an incubation groove. The incubation groove and the inner bottom surface of the laser confocal culture dish together form a closed incubation space.

2. The cell immunofluorescence detection antibody incubation device based on laser confocal culture dish according to claim 1, characterized in that: The incubation tank is recessed upwards along the bottom surface of the incubation cover.

3. The cell immunofluorescence detection antibody incubation device based on laser confocal culture dish according to claim 1, characterized in that: The bottom surface of the incubation cover is a smooth surface that is pressed tightly against the inner bottom surface of the laser confocal culture dish.

4. The cell immunofluorescence detection antibody incubation device based on laser confocal culture dish according to claim 1, characterized in that: An elastic member is connected to the incubation cover, which provides pressure to the inner bottom surface of the laser confocal culture dish.

5. The cell immunofluorescence detection antibody incubation device based on laser confocal culture dish according to claim 4, characterized in that: The elastic member includes an elastic sheet, the first end of which is fixed to the outer top surface of the incubation cover, and the tail end of which is connected to a snap-fit ​​assembly.

6. The cell immunofluorescence detection antibody incubation device based on laser confocal culture dish according to claim 5, characterized in that: The snap-fit ​​assembly includes a buckle fixed to the tail end of the elastic sheet.

7. The cell immunofluorescence detection antibody incubation device based on laser confocal culture dish according to claim 6, characterized in that: The buckle is provided with a snap-fit ​​groove, and the snap-fit ​​inlet and outlet of the snap-fit ​​groove are set downwards.

8. The cell immunofluorescence detection antibody incubation device based on laser confocal culture dish according to claim 7, characterized in that: The width of the snap-fit ​​groove is greater than or equal to the thickness of the sidewall of the laser confocal culture dish.

9. The cell immunofluorescence detection antibody incubation device based on laser confocal culture dish according to claim 1, characterized in that: The incubation cover is cylindrical in shape.

10. The cell immunofluorescence detection antibody incubation device based on laser confocal culture dish according to claim 1, characterized in that: The incubation tank is circular in shape.

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