Full-automatic nucleic acid extractor for pathological tissue slices

Abstract

The utility model provides a full-automatic extraction instrument for pathological tissue slice nucleic acid, which relates to the technical field of extraction instruments and comprises an extraction instrument body, a first mechanical arm, a second mechanical arm and a third mechanical arm are respectively arranged in the extraction instrument body, a dewaxing integrated kit is arranged on one side of the first mechanical arm, and a dewaxing integrated kit is arranged on the other side of the second mechanical arm. A slicing frame is installed on one side of the dewaxing integrated kit, a scraping mechanism is arranged on one side of the second mechanical arm, an EP pipe containing box is installed on one side of the second mechanical arm, a scanning assembly is installed on one side of the EP pipe containing box, and an EP pipe transferring box is installed on one side of the third mechanical arm. In the process of slicing and extracting a nucleic acid sample, manual operation is not needed, mechanical sampling in the whole process is achieved, the sampling efficiency is indirectly improved, pollution possibly caused by manual operation is effectively avoided, the labor cost of workers is reduced, and the practicability is greatly improved through the design.

Description

Technical Field

[0001] This utility model belongs to the field of extraction instrument technology, and more specifically, it relates to a fully automated nucleic acid extraction instrument for pathological tissue sections. Background Technology

[0002] Pathological tissue sections are a type of pathological specimen, prepared by taking a lesion tissue of a certain size and using pathological histological methods. Typically, the lesion tissue is embedded in a paraffin block, cut into thin sections using a microtome, stained with hematoxylin and eosin (HE), and finally examined under a microscope to observe pathological changes and make a pathological diagnosis.

[0003] Based on the above, the inventors have discovered the following problems: In the process of extracting nucleic acid samples, most of the existing extraction instruments are operated manually. However, manual operation may lead to low extraction efficiency and indirectly increase the labor cost for staff, which is quite inconvenient.

[0004] Therefore, in view of this, we have studied and improved the existing structure and its deficiencies, and provided a fully automated nucleic acid extraction instrument for pathological tissue sections, in order to achieve a more practical value. Utility Model Content

[0005] The purpose and efficacy of this utility model, a fully automated nucleic acid extraction instrument for pathological tissue sections, are achieved through the following specific technical means:

[0006] An automated nucleic acid extraction instrument for pathological tissue sections includes an extraction instrument body. The extraction instrument body is internally equipped with robotic arms one, two, and three. A dewaxing kit is mounted on one side of robotic arm one, and a slide holder is mounted on one side of the dewaxing kit. A scraping mechanism is mounted on one side of robotic arm two, and an EP tube placement box is mounted on one side of the EP tube placement box. A scanning component is mounted on one side of robotic arm three. An EP tube transport box is mounted on one side of robotic arm three. The extraction instrument body is internally equipped with a mechanism for moving the EP tube transport box, and an EP tube holder is mounted on the side of robotic arm three away from the EP tube transport box.

[0007] Furthermore, the top of the extractor body is fitted with several sets of L-shaped transparent protective doors via hinges, and a handle is installed on one side of each L-shaped transparent protective door.

[0008] Furthermore, the scraping mechanism includes a support platform with a placement groove on its surface. One end of the support platform is connected to an electric cylinder via a drive shaft, and one end of the electric cylinder is equipped with a scraper, which is located above the placement groove.

[0009] Furthermore, the top of the scanning component is provided with an HE slice mounting slot.

[0010] Furthermore, the mechanism for moving the EP tube transfer box includes a threaded rod, on which a slider is threadedly installed. A groove is provided on one side of the robotic arm three, and one end of the slider passes through the groove and is connected to the EP tube transfer box.

[0011] Furthermore, a drive motor is installed on one side of the extractor body, and the output end of the drive motor passes through the extractor body to the interior and forms a transmission connection with the threaded rod.

[0012] Furthermore, a control panel is provided on one side of the extraction instrument body, and a parameter comparison module is provided inside the control panel.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] Through the cooperation of robotic arms one, two, and three, staff can extract nucleic acid samples without manual operation, achieving fully mechanized sampling. This indirectly improves sampling efficiency, effectively avoids contamination that may be caused by human operation, reduces labor costs for staff, and greatly enhances practicality.

[0015] With the design of the L-shaped transparent protective door, staff can observe the inside of the extraction instrument in real time. When manual operation or internal maintenance is required, the L-shaped transparent protective door can be opened by the handle, making the operation very simple. Attached Figure Description

[0016] Figure 1 This is a three-dimensional schematic diagram of a fully automated nucleic acid extraction instrument for pathological tissue sections according to this utility model.

[0017] Figure 2 This is a three-dimensional schematic diagram of a partial structure of a fully automated nucleic acid extraction instrument for pathological tissue sections according to this utility model.

[0018] Figure 3 This is a three-dimensional schematic diagram of a partial structure of a fully automated nucleic acid extraction instrument for pathological tissue sections according to this utility model.

[0019] Figure 4 This utility model relates to a fully automated nucleic acid extraction instrument for pathological tissue sections. Figure 2 Enlarged view of point A.

[0020] In the diagram, the correspondence between component names and drawing numbers is as follows:

[0021] 1. Extraction instrument body; 2. L-shaped transparent protective door; 3. Handle; 4. Robotic arm one; 5. Slice holder; 6. Dewaxing kit; 7. Scanning component; 8. HE slice mounting slot; 9. EP tube placement box; 10. Slide groove; 11. Drive motor; 12. EP tube transfer box; 13. Robotic arm two; 14. Robotic arm three; 15. EP tube fixing bracket; 16. Threaded rod; 17. Slider; 18. Support platform; 19. Electric cylinder; 20. Scraper; 21. Placement slot; 22. Control panel. Detailed Implementation

[0022] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0023] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In addition, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0024] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances. Example

[0025] As attached Figure 1 To be continued Figure 4 As shown:

[0026] This utility model provides a fully automated nucleic acid extraction instrument for pathological tissue sections, including an extraction instrument body 1. The extraction instrument body 1 is internally equipped with a first robotic arm 4, a second robotic arm 13, and a third robotic arm 14. A dewaxing kit 6 is mounted on one side of the first robotic arm 4, and a slide holder 5 is mounted on one side of the dewaxing kit 6. A scraping mechanism is mounted on one side of the second robotic arm 13, and an EP tube placement box 9 is mounted on one side of the EP tube placement box 9. A scanning component 7 is mounted on one side of the third robotic arm 14. An EP tube is mounted on one side of the third robotic arm 14. The transfer box 12 is provided inside the extractor body 1, which has a mechanism for moving the EP tube transfer box. The EP tube fixing frame 15 is installed on the side of the robotic arm 14 away from the EP tube transfer box 12. Through the cooperation between robotic arm 1, robotic arm 2, and robotic arm 3, the staff does not need to perform manual operation during the extraction of nucleic acid sample slices, realizing fully mechanized sampling, which indirectly improves sampling efficiency, effectively avoids contamination that may be caused by human operation, and reduces labor costs for staff. Moreover, this design greatly improves practicality.

[0027] The top of the extraction instrument body 1 is fitted with several sets of L-shaped transparent protective doors 2 via hinges, and a handle 3 is installed on one side of each L-shaped transparent protective door 2. Through the design of the L-shaped transparent protective door 2, the staff can observe the interior of the extraction instrument body 1 in real time. When manual operation or internal maintenance is required, the L-shaped transparent protective door 2 can be opened through the handle 3, making the operation very simple.

[0028] The scraping mechanism includes a support platform 18, on the surface of which a placement groove 21 is provided. One end of the support platform 18 is connected to an electric cylinder 19 via a drive shaft. One end of the electric cylinder 19 is provided with a scraper 20, which is located above the placement groove 21.

[0029] The scanning component 7 has an HE slice mounting slot 8 at its top.

[0030] The mechanism for moving the EP tube transfer box includes a threaded rod 16, on which a slider 17 is threadedly installed. A groove 10 is provided on one side of the robotic arm 14, and one end of the slider 17 passes through the groove 10 and is connected to the EP tube transfer box 12.

[0031] The extraction instrument body 1 is equipped with a drive motor 11 on one side, and the output end of the drive motor 11 passes through the extraction instrument body 1 to the inside and forms a transmission connection with the threaded rod 16.

[0032] The extraction instrument body 1 has a control panel 22 on one side, and the control panel 22 has a parameter comparison module inside.

[0033] The specific usage and function of this embodiment are as follows:

[0034] Before installation and use, the operator needs to inspect the internal components or structure of the extraction instrument body 1. After the inspection is completed, an external power supply can be connected for normal use. First, the operator opens the L-shaped transparent protective door 2 through handle 3 and sets the internal slide parameters through the control panel 22. Then, the HE slide is placed inside the HE slide mounting slot 8, and the EP tube is placed inside the EP tube placement box 9. Afterward, the operator closes the L-shaped transparent protective door 2 again through handle 3 and inputs the command through the control panel 22. During sampling, the robotic arm 4 clamps the slide holder 5 and places it inside the dewaxing integrated reagent kit 6. (Note that the dewaxing integrated reagent kit 6...) The reagent kit 6 contains a dewaxing solution chamber, a 100% ethanol chamber, an 85% ethanol chamber, and a 70% ethanol chamber. Robotic arm 4 controls the slide holder 5 to sequentially place the slides into the dewaxing solution chamber for 15 minutes, the 100% ethanol chamber for 5 minutes, the 85% ethanol chamber for 2 minutes, and the 70% ethanol chamber for 2 minutes. Then, scanning component 7 scans the HE slides on the HE slide mounting slot 8 and determines the scraping location. Next, robotic arm 13 places the white slides from inside the slide holder 5 into the placement slot 21, simultaneously gripping the EP tube from inside the EP tube placement box 9 and placing it to one side of the white slide. Then, the electric cylinder 19 is activated, and according to the instructions from the parameter comparison module, the electric cylinder 19 is adjusted via the drive shaft. The height of the scraper 20 can be adjusted to effectively control the thickness of the scraped tissue from the white film according to the instructions. This allows the tissue from the white film surface to be scraped into the EP tube. After scraping, the robotic arm 2 13 will place the EP tube into the EP tube transfer box 12. Then, under the action of the drive motor 11, the threaded rod 16 will rotate, which will move the slider 17 to move the EP tube transfer box 12. When the EP tube transfer box 12 moves to one side of the robotic arm 3 14, the robotic arm 3 14 will clamp the EP tube containing the nucleic acid sample inside the EP tube transfer box 12 and place it into the EP tube holder 15. Then, the staff can use the magnetic... The bead extraction kit extracts nucleic acid samples from EP tubes on the EP tube holder 15. Simultaneously, the L-shaped transparent protective door 2 allows staff to observe the interior of the extraction instrument 1 in real time. When manual operation or internal maintenance is required, the L-shaped transparent protective door 2 can be opened via the handle 3, making the operation very simple. Through this process, staff can extract nucleic acid samples without manual intervention, achieving fully mechanized sampling, indirectly improving sampling efficiency, effectively avoiding contamination that may be caused by human operation, reducing labor costs for staff, and significantly enhancing practicality.

[0035] The wiring diagrams of robotic arm 1 (4), scanning component 7, drive motor 11, robotic arm 2 (13), robotic arm 3 (14), and electric cylinder 19 in this utility model are common knowledge in the field. Their working principles are known technologies. The appropriate model is selected according to actual use. Therefore, the control methods and wiring arrangements of robotic arm 1 (4), scanning component 7, drive motor 11, robotic arm 2 (13), robotic arm 3 (14), and electric cylinder 19 will not be explained in detail.

[0036] The embodiments of this utility model are given for illustrative and descriptive purposes only, and are not intended to be exhaustive or to limit the utility model to the forms disclosed. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described in order to better illustrate the principles and practical applications of this utility model, and to enable those skilled in the art to understand this utility model and design various embodiments with various modifications suitable for a particular purpose.

Claims

1. A pathological tissue section nucleic acid automatic extraction instrument, comprising an extraction instrument body (1), characterized in that: The inside of the extraction instrument body (1) is respectively provided with a mechanical arm one (4), a mechanical arm two (13) and a mechanical arm three (14), one side of the mechanical arm one (4) is provided with a dewaxing integrated reagent box (6), one side of the dewaxing integrated reagent box (6) is installed with a slice rack (5), one side of the mechanical arm two (13) is provided with a scraping mechanism, one side of the mechanical arm two (13) is installed with an EP tube placing box (9), one side of the EP tube placing box (9) is installed with a scanning assembly (7), one side of the mechanical arm three (14) is installed with an EP tube transfer box (12), the inside of the extraction instrument body (1) is provided with a mechanism for moving the EP tube transfer box, and one side of the mechanical arm three (14) away from the EP tube transfer box (12) is installed with an EP tube fixing frame (15).

2. The fully automated nucleic acid extraction instrument for pathological tissue sections as described in claim 1, characterized in that: The top end of the extraction instrument body (1) is installed with a plurality of groups of L-shaped transparent protective doors (2) through hinges, and one side of the L-shaped transparent protective door (2) is installed with a handle (3).

3. The fully automated nucleic acid extraction instrument for pathological tissue sections as described in claim 1, characterized in that: The scraping mechanism comprises a supporting table (18), a placing groove (21) is formed in the surface of the supporting table (18), one end of the supporting table (18) is connected and installed with an electric cylinder (19) through a driving shaft, one end of the electric cylinder (19) is provided with a scraper (20), and the scraper (20) is located above the placing groove (21).

4. The fully automated nucleic acid extraction instrument for pathological tissue sections as described in claim 1, characterized in that: The top end of the scanning assembly (7) is provided with an HE slice installation groove (8).

5. The fully automated nucleic acid extraction instrument for pathological tissue sections as described in claim 1, characterized in that: The mechanism for moving the EP tube transfer box comprises a threaded rod (16), a sliding block (17) is installed on the threaded rod (16) through threads, one side of the mechanical arm three (14) is provided with a sliding groove (10), and one end of the sliding block (17) penetrates through the sliding groove (10) and is connected with the EP tube transfer box (12).

6. The fully automated nucleic acid extraction instrument for pathological tissue sections as described in claim 5, characterized in that: One side of the extraction instrument body (1) is installed with a driving motor (11), and the output end of the driving motor (11) penetrates the extraction instrument body (1) to the inside and is in transmission connection with the threaded rod (16).

7. The fully automated nucleic acid extraction instrument for pathological tissue sections as described in claim 1, characterized in that: One side of the extraction instrument body (1) is provided with a control panel (22), and the inside of the control panel (22) is provided with a parameter comparison module.

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