Deep hole plate

Abstract

The utility model discloses a deep hole plate which comprises a shell and a plurality of extraction units assembled in the shell, each extraction unit is composed of a plurality of deep hole grooves, the plurality of extraction units are sequentially arranged in the longitudinal direction, and the deep hole groove of each extraction unit comprises a stirring sleeve hole, a cracking hole, a washing unit and an elution hole which are sequentially arranged in the transverse direction at intervals. The cracking hole is of a long-strip-groove-shaped structure allowing the stirring sleeve to horizontally move, the washing unit comprises a plurality of washing holes, and the volume of the cracking hole is larger than that of the washing holes; the interior of the stirring sleeve hole is longitudinally and sequentially provided with a plurality of stirring sleeve containing cavities at intervals, and the number of the stirring sleeve containing cavities is matched with that of the extraction units; the stirring sleeve is placed in the stirring sleeve hole in advance, so that the additional insertion process of the stirring sleeve is simplified, trouble and labor are saved, and packaging and transportation are facilitated; the cracking hole facilitates cracking of a large-system sample, and the stirring sleeve can horizontally move back and forth while rotating and stirring in the cracking hole, so that full cracking is facilitated.

Description

Technical Field

[0001] This utility model relates to the field of in vitro diagnostic consumables technology, specifically to a deep-well plate adapted to a small-to-medium throughput nucleic acid testing pipeline. Background Technology

[0002] Nucleic acids are the carriers of genetic information, the most important biological information molecules, and a primary object of molecular biology research. Therefore, nucleic acid extraction is the most important and fundamental operation in molecular biology experimental techniques. Compared with traditional nucleic acid extraction methods, magnetic bead-based nucleic acid extraction technology has advantages such as high throughput, automation, and high product purity, and is widely used in various fields such as emergency epidemic prevention and control, clinical disease diagnosis, and molecular biology research. In the process of magnetic bead-based nucleic acid extraction, deep-well plates are the most commonly used consumable container for the lysis, transfer, and storage of test samples, and are compatible with various automated nucleic acid detection instruments.

[0003] Currently, common deep-well plates are 96-well and 384-well plates. For example, Chinese patent CN209412193U describes a stackable 96-well deep-well plate, comprising a plate body and a cover. The cover is positioned above the plate body, and the plate body contains multiple sets of deep holes. As shown in the attached diagram, each hole in this deep-well plate has a uniform volume. Furthermore, the cover is required to stack two deep-well plates during use. Therefore, this type of deep-well plate struggles to simultaneously achieve good mixing and avoid liquid residue issues during magnetic bead processing. Additionally, the number of holes in the deep-well plate cannot meet the needs of flexible detection at small to medium throughput. Another example is Chinese patent CN206279194U, which discloses a novel 24-well deep-well plate with a magnetic sleeve. While this reduces the sample throughput per test, it requires the magnetic rod to be fitted with the sleeve, and the uniform volume of all 24 holes makes the operation cumbersome and the extraction efficiency poor. For example, Chinese patent number CN206529485U discloses a deep-hole plate structure for enhancing mass transfer. It proposes to enhance the mixing effect of liquid in narrow deep holes by setting baffles at the bottom of the holes of existing deep-hole plates or by using stirring balls. However, the above design is prone to causing residue of extracted products inside the deep-hole plate, reducing product yield. At the same time, obstacles are set inside each hole, which inevitably increases the processing difficulty and production cost.

[0004] In summary, those skilled in the art urgently need to develop a deep-well plate adapted to low-to-medium throughput nucleic acid testing pipelines to address the shortcomings of existing technologies. Utility Model Content

[0005] The purpose of this invention is to address the aforementioned problems by providing a deep-well plate. This plate integrates a stirring sleeve hole, a lysis hole, a washing hole, and an elution hole, all housed within the plate. The stirring sleeve is pre-placed within the stirring sleeve hole, allowing for direct nucleic acid extraction. This simplifies the additional insertion process of the stirring sleeve, saving time and effort, and facilitating packaging and transportation. Furthermore, the deep-well plate's elongated, groove-shaped lysis holes not only meet the lysis requirements of large-scale sample systems but also allow the stirring sleeve to rotate and move horizontally within the lysis holes, promoting thorough lysis.

[0006] The technical solution adopted in this utility model is as follows:

[0007] A deep-hole plate includes a housing and multiple extraction units assembled within the housing. Each extraction unit is composed of multiple deep-hole grooves, arranged longitudinally. Each extraction unit's deep-hole groove includes, in a transverse direction, mutually spaced stirring sleeve holes, pyrolysis holes, washing units, and elution holes. The pyrolysis holes are elongated groove-shaped structures that allow the stirring sleeve to move horizontally. The washing unit includes several washing holes, and the volume of the pyrolysis holes is larger than the volume of the washing holes. The interior of each stirring sleeve hole is longitudinally arranged with multiple mutually spaced stirring sleeve receiving cavities. The number of stirring sleeve receiving cavities matches the number of extraction units, and each stirring sleeve receiving cavity can be used to hold a single stirring sleeve.

[0008] Furthermore, the bottom of the pyrolysis hole has a flat bottom structure.

[0009] Furthermore, the bottom end of the elution hole has a conical structure.

[0010] Furthermore, the upper parts of the stirring sleeve receiving cavities are interconnected, the middle part of the stirring sleeve receiving cavity and the side wall corresponding to the adjacent stirring sleeve receiving cavity are shared wall structures, the lower end of the stirring sleeve receiving cavity is an independent non-shared wall structure, and a stirring sleeve is placed inside the stirring sleeve receiving cavity.

[0011] Furthermore, each of the stirring sleeve receiving cavities has multiple vertical ridges evenly distributed circumferentially, and the multiple vertical ridges cooperate with each other to vertically restrict the stirring sleeve within the stirring sleeve receiving cavity; the upper part of the stirring sleeve receiving cavity adjacent to the outer shell is also provided with an outwardly extending clearance structure.

[0012] Furthermore, the height of the stirring sleeve receiving cavity is greater than the height of the stirring sleeve; the lower outer wall of the stirring sleeve receiving cavity is also provided with reinforcing ribs.

[0013] Furthermore, the opening end of the deep hole groove is higher than the outer shell to form a protruding ridge; the bottom of the outer shell is provided with a base, and the bottom end of the base is provided with a groove platform that can cooperate with the protruding ridge.

[0014] Furthermore, the sidewalls of the housing are provided with slots that can match the mechanical grippers, and at least a pair of slots are symmetrically arranged on opposite sides of the housing.

[0015] Furthermore, several transition ribs are provided at the junction of the outer shell and the base.

[0016] Furthermore, the stirring sleeve hole, pyrolysis hole, washing hole, and elution hole each include an upper end and a lower end. The upper end is a rectangular opening structure, and the sidewalls of adjacent upper ends are shared. The sidewalls of the lower ends of the stirring sleeve hole, pyrolysis hole, washing hole, and elution hole are independent non-shared wall structures.

[0017] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0018] 1. This utility model sets the stirring sleeve hole together with the lysis hole, washing hole and elution hole in the deep well plate. At the same time, the stirring sleeve is pre-placed in the stirring sleeve hole, which can be directly put into the machine to complete the nucleic acid extraction work. This simplifies the extra insertion process of the stirring sleeve, saves time and effort, and facilitates packaging and transportation.

[0019] 2. The deep hole plate provided by this utility model, by setting mutually spaced stirring sleeve receiving cavities and reserving a certain amount of clearance structure, can not only ensure that the stirring sleeve is placed vertically in it, but also facilitate the gripping of the stirring sleeve.

[0020] 3. The height of the mixing sleeve cavity of this utility model is greater than the height of the mixing sleeve, which avoids the mixing sleeve protruding from the surface of the deep hole plate, making it convenient to carry out the coating process on the surface of the deep hole plate.

[0021] 4. The deep-hole plate provided by this utility model has a long groove-shaped pyrolysis hole with a large volume that not only meets the pyrolysis requirements of large system samples, but also facilitates the horizontal back-and-forth movement of the stirring sleeve while it rotates and stirs in the pyrolysis hole, which is conducive to thorough pyrolysis.

[0022] 5. The present invention designs the bottom of the pyrolysis hole as a flat-bottom structure, which can reduce the liquid level while keeping the volume of the pyrolysis system unchanged, thereby achieving a certain anti-contamination effect. At the same time, the flat-bottom design of the bottom of the pyrolysis hole can also increase the contact area between the liquid in the pyrolysis hole and the heating block, thereby improving the heating efficiency accordingly.

[0023] 6. The deep well plate provided by this utility model can be set with any number of extraction units according to needs to meet the application requirements of small and medium throughput nucleic acid detection pipelines, making it more flexible to use, more efficient and able to produce results quickly.

[0024] 7. This utility model has a simple overall structure, good compatibility, and low manufacturing cost, and has good application prospects. Attached Figure Description

[0025] Figure 1 A perspective view of the deep hole plate provided by this utility model;

[0026] Figure 2 A top view of the deep hole plate provided by this utility model;

[0027] Figure 3 A cross-sectional view of the deep hole plate provided by this utility model;

[0028] Figure 4 A partial structural diagram of the interior of the stirring sleeve hole of the deep hole plate provided by this utility model;

[0029] Figure 5 A schematic diagram of the bottom structure of the deep hole plate provided by this utility model;

[0030] Figure 6 A perspective view of the stirring sleeve placed inside the deep hole plate provided by this utility model;

[0031] Figure 7 The cross-section of the deep-hole plate provided by this utility model for holding the stirring sleeve. Figure 1 ;

[0032] Figure 8 A perspective view of multiple deep-hole plates provided by this utility model stacked together;

[0033] Figure 9 The cross-section of the deep-hole plate provided by this utility model for holding the stirring sleeve. Figure 2 ;

[0034] Reference numerals: 10-outer shell, 11-groove, 12-protruding ridge, 13-base, 14-foolproof part, 15-transition rib, 16-grooved platform, 20-stirring sleeve hole, 201-vertical ridge, 202-baffle, 203-void-proof structure, 204-reinforcing rib, 21-cracking hole, 22-washing unit, 221-first washing hole, 222-second washing hole, 223-third washing hole, 23-elution hole, 24-stirring sleeve. Detailed Implementation

[0035] The present invention will now be described in detail with reference to the accompanying drawings.

[0036] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0037] Example 1

[0038] This utility model provides a deep-well plate adapted to low-to-medium throughput nucleic acid testing pipelines. Please refer to [link to relevant documentation]. Figure 1 , Figure 2 , Figure 3 , Figure 4 The device includes a base 13, a shell 10, and several deep-hole grooves. These deep-hole grooves are arranged horizontally in a series of spaced-apart stirring sleeve holes 20, lysis holes 21, washing units 22, and elution holes 23. Each horizontally arranged stirring sleeve hole 20, lysis hole 21, washing unit 22, and elution hole 23 forms an extraction unit, enabling the extraction of nucleic acid from a sample. Specifically, a certain amount of sample is added to the lysis hole 21 to contact the lysis buffer inside, achieving the release of nucleic acid and its binding with magnetic beads. The washing unit 22 is used to wash away excess impurities and includes several horizontally arranged washing holes. Then, the thoroughly washed nucleic acid is separated from the magnetic beads in the elution hole 23. In this embodiment, the number of extraction units is four. Compared to high-throughput nucleic acid extraction deep-well plates, the deep-well plate provided in this embodiment is suitable for application scenarios of small-to-medium throughput nucleic acid detection pipelines. It eliminates the need for staff to wait longer, enabling faster result output. Furthermore, it offers more flexible and convenient operation, avoiding the waste of consumables caused by using high-throughput nucleic acid extraction deep-well plates when the sample quantity is small. The stirring sleeve hole 20 is used to house the stirring sleeve 24, facilitating packaging and transportation, and eliminating the need for staff to manually add stirring sleeves one by one after installing the deep-well plate on the extractor. To avoid mutual interference between multiple stirring sleeves 24, the interior of the stirring sleeve hole 20 is longitudinally arranged with multiple spaced-apart stirring sleeve receiving cavities. In this embodiment, the number of stirring sleeve receiving cavities is four, matching the number of extraction units. Figure 4 and Figure 9 As shown, adjacent stirring sleeve cavities are separated by baffles 202. Specifically, the highest point of baffle 202 is lower than the highest point of the transition section between the upper and lower sections of the stirring sleeve 24. Here, the upper section of the stirring sleeve is used for assembly with the equipment, and the lower section is used for stirring. Therefore, the diameter of the upper section is larger than that of the lower section, and a transition section is formed between the upper and lower sections. The highest point of baffle 202 is located at the transition section, which can avoid the upper section of the stirring sleeve, so that the distance between the upper sections of the adjacent stirring sleeves is less than the thickness of baffle 202. This allows multiple stirring sleeves to be compactly arranged in the stirring sleeve holes 20, which correspondingly reduces the gaps of other deep-cavity grooves in the extraction unit, achieving a compact design. Each stirring sleeve cavity can hold one stirring sleeve, and each stirring sleeve corresponds to one extraction unit. Specifically, the upper parts of the stirring sleeve cavities are interconnected, the middle part of the stirring sleeve cavity and the sidewalls corresponding to the adjacent stirring sleeve cavities are shared-wall structures, and the lower ends of the stirring sleeve cavities are independent non-shared-wall structures. In addition, in order to meet the nucleic acid extraction requirements of large-volume samples, such as Figure 3 and Figure 4 As shown, the volume of the lysis well 21 is greater than the volume of the washing well. Specifically, the length of the bottom groove of the lysis well 21, i.e., the distance between AA', is 19±0.1mm; the width of the bottom groove of the lysis well 21, i.e., the distance between BB', is 5.8±0.1mm; and the height of the bottom groove of the lysis well 21, i.e., the distance between CC', is 9.4±0.1mm. Accordingly, it can achieve sufficient lysis of a system with a sample volume and a total volume of lysis reagent of ≤5ml, and the washing well can achieve washing of a 1ml system.

[0039] To reduce the risk of cross-contamination, such as Figure 3 As shown, the stirring sleeve hole 20, the pyrolysis hole 21, the washing unit 22, and the elution hole 23 all include an upper end and a lower end. The upper end is a rectangular opening structure, and the sidewalls of adjacent upper ends are a common wall structure. The sidewalls of the lower ends of the stirring sleeve hole 20, the pyrolysis hole 21, the washing unit 22, and the elution hole 23 are independent non-common wall structures.

[0040] like Figure 3 and Figure 5 As shown, the bottom of the lysis orifice 21 and the washing unit 22 are flat-bottomed structures. This allows for a larger volume while maintaining the same lysis system volume, effectively reducing the liquid level and achieving a certain level of anti-contamination. Furthermore, with the same heating block enclosure height, the flat-bottom design of the lysis orifice 21 increases the contact area between the liquid inside the lysis orifice and the heating block, thereby improving heating efficiency. The bottom of the elution orifice 23 is a conical bottom structure, facilitating the sampler's deep insertion for sampling and preventing liquid residue. In addition, the volume of the lysis orifice 21 is significantly larger than that of the adjacent washing orifice. This design allows the stirring sleeve to rotate within the lysis orifice 21 while moving horizontally back and forth, enhancing mixing and achieving thorough sample lysis.

[0041] like Figure 4As shown, to ensure that each stirring sleeve inserted into the stirring sleeve hole 20 can be placed vertically to avoid tilting and facilitate the gripping and installation of the stirring sleeve, vertical ribs 201 are symmetrically arranged around the inner wall of the middle part of each stirring sleeve receiving cavity. The distance between the mutually symmetrical vertical ribs 201 inside each stirring sleeve receiving cavity is 5.8±0.05mm. To prevent the stirring sleeve from deforming outward and getting stuck when gripped, a clearance structure 203 is also provided on the upper part of the stirring sleeve receiving cavity adjacent to the outer shell 10. Specifically, the width of the upper opening end of the stirring sleeve hole 20, i.e., the distance between DD', is 10.6±0.2mm, and the diameter of the outermost ring of the opening end of the stirring sleeve is 8.3±0.2mm. The clearance structure 203 is an annular groove with a certain depth. The annular groove matches the upper pipe section of the stirring sleeve. The clearance structure 203 can also prevent the colloid from overflowing onto the stirring sleeve placed inside the stirring sleeve hole 20 during the coating process of the deep hole plate, which would cause the stirring sleeve to get stuck when gripping.

[0042] like Figure 2 As shown, in the above-mentioned deep hole plate, in order to facilitate the staff to identify the installation direction of the deep hole plate, the outer shell 10 is also provided with a foolproof part 14.

[0043] like Figure 3 and Figure 1 As shown, to facilitate the stacking of multiple deep-hole plates, the opening end of the deep-hole groove is higher than the outer shell 10, forming a protruding ridge 12; correspondingly, a groove platform 16 that can cooperate with the protruding ridge 12 is provided at the bottom end of the base 13 of the deep-hole plate. In actual use, as... Figure 8 As shown, the groove 16 of one deep-hole plate is fastened to the protrusion 12 of another deep-hole plate, enabling the stacking of two or more deep-hole plates, thereby reducing the area occupied by the deep-hole plates. Furthermore, as... Figure 6 and Figure 7 As shown, the height of the stirring sleeve hole 20 is greater than the height of the stirring sleeve 24. Specifically, the distance between the top of the stirring sleeve 24 and the opening end of the stirring sleeve hole 20 is ≥1.3mm. This design can prevent the stirring sleeve from protruding outside the deep well groove, making it convenient to perform the coating process on the surface of the deep well plate. This completely wraps the stirring sleeve in the stirring sleeve hole 20, ensuring the flatness of the deep well plate surface and facilitating subsequent transportation. At the same time, after the nucleic acid extraction process is completed, the coating process can still be performed on the surface of the deep well plate, which reduces the problem of contamination compared to directly discarding the deep well plate.

[0044] In addition, in order to facilitate the gripping and movement of the deep well plate by the mechanical gripper of the nucleic acid extraction instrument, in this embodiment, the four sides of the outer shell 10 are provided with slots 11 that can match the mechanical gripper, and the slots 11 on the opposite side walls are symmetrically arranged.

[0045] In the aforementioned deep-hole plate, the washing unit 22 has three washing holes in this embodiment, namely, a first washing hole 221, a second washing hole 222, and a third washing hole 223 arranged sequentially in the transverse direction. Specifically, the first washing hole 221, the second washing hole 222, and the third washing hole 223 have the same structure and can all achieve washing of a 1ml system.

[0046] In addition, such as Figure 1 As shown, a transition rib 15 is also provided at the junction of the bottom end of the deep hole plate outer shell 10 and the base 13 to prevent overlapping and unevenness between multiple stacked deep hole plate sets. To prevent the outer ring from deforming inwards, as... Figure 5 The lower outer wall of the stirring sleeve cavity of the deep hole plate provided in this embodiment is also provided with reinforcing ribs 204, which serve as supports.

[0047] To facilitate processing and manufacturing, the deep hole plate provided in this embodiment can be prepared using injection molding, and the material of the deep hole plate can be polypropylene.

[0048] This document uses specific embodiments to illustrate the principles and implementation methods of this utility model. The descriptions of the embodiments above are only for the purpose of helping to understand the method and core ideas of this utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made to this utility model without departing from the principles of this utility model, and these improvements and modifications also fall within the protection scope of the claims of this utility model.

[0049] In the description of this utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the utility model product is in use. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0050] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

Claims

1. A deep-hole plate, comprising a housing and a plurality of extraction units assembled within the housing, wherein each extraction unit is composed of a plurality of deep-hole grooves, characterized in that, Multiple extraction units are arranged longitudinally. Each extraction unit has a deep-hole groove comprising a stirring sleeve hole, a pyrolysis hole, a washing unit, and an elution hole arranged transversely and spaced apart. The pyrolysis hole is a long, groove-shaped structure that allows the stirring sleeve to move horizontally. The washing unit includes several washing holes, and the volume of the pyrolysis hole is larger than the volume of the washing holes. The interior of the stirring sleeve hole is longitudinally configured with multiple, spaced-apart stirring sleeve receiving cavities. The number of stirring sleeve receiving cavities matches the number of extraction units, and each stirring sleeve receiving cavity can be used to hold a single stirring sleeve.

2. The deep hole plate as described in claim 1, characterized in that, The bottom of the pyrolysis hole has a flat bottom structure.

3. The deep hole plate as described in claim 1, characterized in that, The bottom of the elution hole has a conical structure.

4. The deep hole plate according to any one of claims 1-3, characterized in that, The upper parts of the stirring sleeve receiving cavities are interconnected, the middle part of the stirring sleeve receiving cavity and the side wall corresponding to the adjacent stirring sleeve receiving cavity are shared wall structures, the lower end of the stirring sleeve receiving cavity is an independent non-shared wall structure, and a stirring sleeve is placed inside the stirring sleeve receiving cavity.

5. The deep hole plate as described in claim 4, characterized in that, Each of the stirring sleeve receiving cavities has multiple vertical ridges evenly distributed circumferentially. The multiple vertical ridges cooperate with each other to vertically restrict the stirring sleeve within the stirring sleeve receiving cavity. The upper part of the stirring sleeve receiving cavity adjacent to the outer shell is also provided with an outwardly extending clearance structure.

6. The deep hole plate as described in claim 4, characterized in that, The height of the stirring sleeve cavity is greater than the height of the stirring sleeve; the lower outer wall of the stirring sleeve cavity is also provided with reinforcing ribs.

7. The deep hole plate as described in claim 1, characterized in that, The opening end of the deep hole groove is higher than the outer shell to form a protruding ridge; the bottom of the outer shell is provided with a base, and the bottom end of the base is provided with a groove platform that can cooperate with the protruding ridge.

8. The deep hole plate as described in claim 1, characterized in that, The sidewalls of the housing are provided with slots that can match mechanical grippers, and at least a pair of slots are symmetrically arranged on opposite sides of the housing.

9. The deep hole plate as described in claim 8, characterized in that, Several transition ribs are also provided at the junction of the outer shell and the base.

10. The deep hole plate as described in claim 1, characterized in that, The stirring sleeve hole, pyrolysis hole, washing hole, and elution hole all include an upper end and a lower end. The upper end is a rectangular opening structure, and the sidewalls of adjacent upper ends are shared. The sidewalls of the lower ends of the stirring sleeve hole, pyrolysis hole, washing hole, and elution hole are independent non-shared wall structures.

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