High-throughput single cell screening and purifying device

Abstract

The utility model discloses a high-flux single cell screening and purifying device which comprises a purifying mechanism, the purifying mechanism comprises a constant-temperature box, two guide sleeves installed on one side of the constant-temperature box, an experiment table connected between the two guide sleeves in a sliding mode, and two supporting legs movably connected with the bottom of the experiment table. According to the utility model, after the experiment table is horizontally placed, the supporting legs are opened, the experiment table is moved downwards to enable the supporting legs to be attached to the table top, then the constant temperature box is opened, a culture dish containing samples in the constant temperature box is directly placed on the experiment table for observation, the samples are qualified if the colors of the samples are white, other colors of the samples are not qualified, and then an alcohol lamp is used for performing over-fire sterilization on an inoculating loop to complete sterile treatment. The method comprises the following steps: firstly, preparing a culture dish, then dividing the culture dish into four areas by adopting a flat plate scribing method, completing inoculation, and finally putting the culture dish into a constant-temperature box for 1-2 days, so that repeated carrying of the culture dish is avoided and the working efficiency is remarkably improved due to the collaborative design of an experiment table and the constant-temperature box.

Description

Technical Field

[0001] This utility model relates to the field of purification device technology, specifically a high-throughput single-cell screening and purification device. Background Technology

[0002] Cell purification refers to the technical process of isolating specific cell types from complex biological samples (such as tissues, blood, or mixed cell cultures) to obtain a high-purity target cell population. Its core purpose is to remove impurity cells (such as fibroblasts, erythrocytes, and other immune cells) to ensure the accuracy and reliability of subsequent experiments or treatments.

[0003] Before performing single-cell purification, the inoculation loop needs to be heated with an alcohol lamp. Then, a single colony is picked and inoculated onto a good culture dish. Next, the streak plate method is used to draw four regions in the culture dish. After completion, the culture dish is placed in an incubator for 24-48 hours to complete the purification process. Throughout the process, the experimenter needs to move the culture dish back and forth, which not only reduces work efficiency but also makes it easy for the culture dish to fall, causing the purification process to be interrupted. Utility Model Content

[0004] This utility model aims to solve one of the technical problems existing in the prior art or related technologies.

[0005] Therefore, the technical solution adopted by this utility model is as follows:

[0006] A high-throughput single-cell screening and purification device includes a purification mechanism, which includes a constant temperature chamber, two guide sleeves installed on one side of the constant temperature chamber, an experimental platform slidably connected between the two guide sleeves, two support legs movably connected to the bottom of the experimental platform, and an alcohol lamp movably connected to the experimental platform via a rotating shaft. The experimental platform has an opening suitable for the rotation of the alcohol lamp.

[0007] By adopting the above technical solution, after placing the experimental table horizontally, the support legs are opened, and then the experimental table is lowered so that the support legs are in contact with the tabletop. Then, the incubator is opened, and the petri dishes containing the samples are placed directly on the experimental table for observation. White samples are considered acceptable, while other colors are unacceptable. The inoculation loop is then sterilized by passing it over an alcohol lamp to complete the aseptic process. The streak plate method is then used to divide the petri dish into four areas for inoculation. Finally, the petri dishes are placed in the incubator for 1 to 2 days. The coordinated design of the experimental table and the incubator avoids repeated handling of the petri dishes and significantly improves work efficiency.

[0008] In a preferred embodiment, the present invention can be further configured such that: notches are provided on both the front and rear sides of the experimental platform, and the length of the notches is equal to the height of the guide sleeve.

[0009] In a preferred embodiment, the present invention can be further configured such that the support legs are shaped like a "「", with the bottom ends of the two support legs located on the same horizontal plane.

[0010] In a preferred embodiment, the present invention can be further configured such that a gap is formed between the constant temperature chamber and the experimental table, the width of which is greater than the diameter of the alcohol lamp body.

[0011] In a preferred embodiment, the present invention can be further configured such that: a plug is installed at the top of the support leg, and the top of the plug is movably engaged with the bottom of the experimental platform.

[0012] In a preferred embodiment, the present invention can be further configured such that: a handle is provided inside the spacing, and the handle is fixedly connected to the experimental table.

[0013] In a preferred embodiment, the present invention can be further configured such that: a slot is provided on one side of the constant temperature chamber, the slot is located between two guide sleeves, and the slot is suitable for the support leg to engage.

[0014] By adopting the above technical solution, the beneficial effects achieved by this utility model are as follows:

[0015] 1. In this utility model, after placing the experimental table horizontally, the support legs are opened, and then the experimental table is lowered so that the support legs are in contact with the tabletop. Then, the incubator is opened, and the culture dish containing the sample is placed directly on the experimental table for observation. If the sample color is white, it is qualified; other colors are unqualified. Then, the inoculation loop is sterilized by passing it over an alcohol lamp to complete the aseptic treatment. Then, the streak plate method is used to divide the culture dish into four areas to complete the inoculation. Finally, the culture dish is placed in the incubator for 1 to 2 days. The coordinated design of the experimental table and the incubator avoids repeated handling of the culture dish and significantly improves work efficiency.

[0016] 2. In this invention, because the top of the alcohol lamp is small and the bottom is large, the bottom of the alcohol lamp will always face downwards, regardless of whether the experimental table is placed vertically or horizontally. This prevents alcohol leakage from the alcohol lamp on the wall and ensures experimental safety. Attached Figure Description

[0017] Figure 1 This is a perspective view of the overall structure of this utility model;

[0018] Figure 2 This is a schematic diagram of the purification mechanism of this utility model;

[0019] Figure 3 This is a schematic diagram showing the disassembly relationship between the guide sleeve and the experimental platform of this utility model;

[0020] Figure 4 This is a schematic diagram showing the connection relationship between the insertion rod and the support leg of this utility model.

[0021] Figure label:

[0022] 100. Purification mechanism; 110. Incubator; 120. Guide sleeve; 130. Laboratory table; 140. Support leg; 150. Alcohol lamp;

[0023] 200. Insert rod;

[0024] 300. Holding hands;

[0025] 400, Groove. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to specific embodiments and accompanying drawings. It should be noted that, unless otherwise specified, the embodiments and features of the present utility model can be combined with each other.

[0027] It should be understood that these descriptions are merely exemplary and not intended to limit the scope of this invention.

[0028] The following describes, with reference to the accompanying drawings, some embodiments of the high-throughput single-cell screening and purification apparatus provided by this utility model.

[0029] Example 1:

[0030] Combination Figure 1-4 As shown, the high-throughput single-cell screening and purification device provided by this utility model includes a purification mechanism 100. The purification mechanism 100 includes a constant temperature chamber 110, two guide sleeves 120 installed on one side of the constant temperature chamber 110, an experimental platform 130 slidably connected between the two guide sleeves 120, two support legs 140 movably connected to the bottom of the experimental platform 130, and an alcohol lamp 150 movably connected to the experimental platform 130 through a rotating shaft. The experimental platform 130 has an opening suitable for the rotation of the alcohol lamp 150.

[0031] Furthermore, the support leg 140 is configured in the shape of "「", with the bottom ends of the two support legs 140 located on the same horizontal plane. The structural design of the support leg 140 ensures that the experimental table 130 can be placed firmly and flat.

[0032] Furthermore, a plug rod 200 is installed at the top of the support leg 140. The top of the plug rod 200 is movably engaged with the bottom of the experimental table 130. The plug rod 200 is provided to ensure that the support leg 140 does not wobble when supporting the experimental table 130.

[0033] Furthermore, a slot 400 is provided on one side of the constant temperature chamber 110. The slot 400 is located between two guide sleeves 120. The slot 400 is suitable for engaging with the support leg 140. The slot 400 provides conditions for retracting the experimental table 130 and the support leg 140.

[0034] Example 2:

[0035] Combination Figure 1-3 As shown, based on Embodiment 1, the experimental platform 130 has notches on both the front and rear sides. The length of the notches is equal to the height of the guide sleeve 120. The notches are provided to ensure that the experimental platform 130 can be retracted smoothly.

[0036] Example 3:

[0037] Combination Figure 1-2 As shown, in the above embodiment, a gap is formed between the constant temperature chamber 110 and the experimental table 130. The width of the gap is greater than the diameter of the alcohol lamp 150. The gap is set to ensure that the alcohol lamp 150 can be placed smoothly, and at the same time to provide installation space for the handle 300.

[0038] Furthermore, a handle 300 is provided inside the gap, and the handle 300 is fixedly connected to the experimental table 130. With the handle 300 provided, the experimental table 130 can be easily pulled up when it is placed vertically, thereby improving the comfort of use.

[0039] Working principle and usage process of this utility model:

[0040] A. Initial preparation: Place the constant temperature chamber 110 on the table, and keep the experimental table 130 in a vertical position;

[0041] B. Device debugging: Flip the experimental platform 130 to a horizontal position, unfold the support leg 140, and fix the insertion rod 200 to the bottom of the experimental platform. Then, move the experimental platform 130 down along the guide sleeve 120 until the bottom of the support leg 140 is completely in contact with the tabletop to ensure stability.

[0042] C. Sample testing: Open the constant temperature incubator 110, take out the petri dish containing the sample, place it directly on the experimental table 130, and then observe the sample color. White is qualified, and other colors are unqualified.

[0043] D. Inoculation procedure: Sterilize the inoculation loop by passing it over an alcohol lamp at 150°C to complete the aseptic treatment. Then, use the streak plate method to divide the petri dish into four areas to complete the inoculation.

[0044] E. Cultivation and observation: Place the culture dish back into the incubator at 110°C and observe the results after standing for 24-48 hours.

[0045] The coordinated design of the experimental platform and the incubator avoids the repeated handling of petri dishes, significantly improving work efficiency.

[0046] Although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A high-throughput single cell screening purification device, characterized in that, Include: Purification mechanism (100), the purification mechanism (100) includes thermostat (110), two guide sleeves (120) installed on one side of the thermostat (110), the experimental table (130) slidingly connected between two guide sleeves (120), two support legs (140) movably connected with the bottom of the experimental table (130), alcohol lamp (150) movably connected with the experimental table (130) through the pivot, the opening is set on the experimental table (130) for the rotation of alcohol lamp (150).

2. The high-throughput single-cell screening and purification device of claim 1, wherein, The front and rear sides of the experimental table (130) are both provided with notches, and the length of the notches is equal to the height of the guide sleeve (120).

3. The high-throughput single-cell screening and purification device of claim 1, wherein, The support leg (140) is arranged in a " " shape, and the bottom ends of the two support legs (140) are located on the same horizontal plane.

4. The high-throughput single-cell screening and purification device of claim 1, wherein, The distance is formed between the thermostat (110) and the experimental table (130), and the width of the distance is greater than the diameter of the lamp body of the alcohol lamp (150).

5. The high-throughput single-cell screening and purification device of claim 1, wherein, The top end of the support leg (140) is provided with an insertion rod (200), and the top end of the insertion rod (200) is movably connected with the bottom of the experimental table (130).

6. The high-throughput single-cell screening and purification device of claim 4, wherein, The inside of the distance is provided with a handle (300), and the handle (300) is fixedly connected with the experimental table (130).

7. The high-throughput single-cell screening and purification device of claim 1, wherein, The side of the thermostat (110) is provided with a notch (400), the notch (400) is located between the two guide sleeves (120), and the notch (400) is suitable for the clamping of the support leg (140).

Images