Separated square culture dish

Abstract

The separated square culture dish comprises a dish body with an opening in the upper portion and a cover body used for sealing the opening, a plurality of partition plates are arranged in the dish body at intervals and divide the dish body into a plurality of long-strip-shaped culture cavities, and a through hole communicated with the culture cavities on the two sides of the partition plate is formed in the bottom or the position close to the bottom of each partition plate. The utility model not only solves the problem that the common culture dish is easy to cross when the liquid is dropped into the culture dish and inclines, but also improves the experiment efficiency and ensures the reliability of the experiment result, and has the characteristics of simple structure, low cost, convenience and practicability.

Description

Technical Field

[0001] This invention provides a partitioned square petri dish, specifically relating to experimental equipment for culturing microorganisms on solid culture media, and belongs to the field of experimental equipment technology. Background Technology

[0002] In the development of bacterial vaccines, it is usually necessary to evaluate the immunogenicity of serum after immunization of animals or humans. A key experiment is to detect the level of vaccine-induced bactericidal antibody titers against the target bacteria in the serum. In the bactericidal antibody titer detection experiment, bacteria, complement, and serially diluted serum are typically mixed first. The incubated mixture is then added dropwise to a culture plate containing a specific antibiotic. The plate is tilted to allow the liquid to flow in a strip, and after incubation for the set experimental time, surviving bacteria form visible colonies. The number of surviving colonies is obtained using an automatic counter or manually. The bactericidal titer is defined as the highest serum dilution that kills 50% of the bacteria, based on the colony count of the wells containing complement without serum.

[0003] However, in the actual operation of the above experiments, ordinary round petri dishes can only use the wider middle part, and usually only a limited number of mixed droplets can be added, resulting in low detection throughput. In contrast, when ordinary square petri dishes are tilted after adding mixed droplets, it is usually difficult to ensure that the droplets flow steadily and evenly to form a uniformly distributed colony band. Droplets at different positions are very likely to cross after tilting, resulting in unclear colony bands after incubation, which seriously affects the judgment of experimental results.

[0004] Therefore, a culture dish suitable for the above experimental requirements is needed to solve the above problems.

[0005] In existing technologies, most segmented porous culture dishes use a grid-like arrangement of culture wells. Even those with a few strip-shaped wells often employ a two-dimensional distribution. Using these existing well types requires sequentially adding culture medium to each well, a cumbersome process that reduces experimental efficiency and can lead to inconsistent amounts of culture medium in each well, affecting subsequent experimental results. This is especially problematic when using heat-melted solid culture medium, as inconsistent solidification times can further impact experimental outcomes. For example, utility model CN219385111U discloses a culture dish that simplifies the experiment by adding a separable partition to the culture chamber, improving efficiency. However, its grid-like arrangement of culture wells is unsuitable for detecting bactericidal antibody titers. Therefore, existing technologies do not provide solutions to the aforementioned or related problems. Utility Model Content

[0006] The purpose of this invention is to utilize a system of partitions within a petri dish, spaced apart to divide the dish into several strip-shaped culture chambers. During use, droplets are poured into different culture chambers, solving the problem of droplets from different positions easily crossing when tilted in ordinary square petri dishes used for antibacterial antibody titer detection, leading to unclear colony bands and severely affecting the interpretation of experimental results. This ensures the reliability of experimental results. Furthermore, this invention provides through-holes at the bottom or near the bottom of each partition, connecting the culture chambers on both sides. This allows the poured culture medium to flow through these holes between different culture chambers and spread to the same liquid level, facilitating the pouring of melted solid culture medium into the dish in one go. It eliminates the need to pour liquid into each culture chamber individually, resulting in a uniform culture medium thickness and solidification time in each chamber of the final culture plate. This significantly simplifies the operation of adding culture medium into the dish, improving experimental efficiency and the quality of the obtained culture plates.

[0007] This utility model is achieved through the following technical solution:

[0008] A partitioned square culture dish includes a dish body with an opening at the top and a lid for sealing the opening. Several partitions are provided inside the dish body, and the partitions are spaced apart inside the dish body to divide the dish body into several elongated culture chambers. Each partition has a through hole at the bottom or near the bottom that connects the culture chambers on both sides of the partition.

[0009] Preferably, the partition is arranged parallel to the side wall of the dish, and the culture chambers formed by the partition are of equal volume.

[0010] Preferably, the spacing between adjacent partitions is 5-25 mm, and more preferably 10-20 mm.

[0011] Preferably, the distance between the top of the partition and the opening of the dish is L, where 0≤L≤2mm, and more preferably, 0<L≤1mm.

[0012] Preferably, the height of the through hole is no more than one-third of the depth of the dish.

[0013] Preferably, the height of the through hole is 2-10 mm, and more preferably 2-5 mm.

[0014] Preferably, the through hole is square or circular in shape, with square being the preferred shape.

[0015] Preferably, all through holes have the same height.

[0016] Preferably, each partition plate has 1 to 10 through holes, more preferably 2 to 5, and even more preferably 2 to 3.

[0017] Preferably, the through holes on the same partition are evenly distributed.

[0018] Preferably, the through holes on adjacent partitions are correspondingly arranged to form liquid flow channels.

[0019] Preferably, both the dish body and the partition are made of plastic or glass.

[0020] Preferably, the partition is integrally formed with the dish body.

[0021] Compared with the prior art, this utility model has the following advantages and beneficial effects:

[0022] (1) This utility model divides the dish into several long strip-shaped culture chambers by setting several partitions in the dish body and the partitions are spaced apart in the dish body, thereby ensuring that the colony bands formed in the culture chamber during the experiment are clear and do not cross, thus ensuring the reliability of the experimental results.

[0023] (2) By setting through holes at the bottom or near the bottom of each partition to connect the culture chambers on both sides of the partition, the present invention facilitates the staff to pour the heated culture medium at one time. The poured culture medium can flow and spread freely between different culture chambers, eventually reaching the same liquid level. After cooling, it solidifies at the same time. This not only greatly simplifies the staff's operation steps and improves the experimental efficiency, but also improves the consistency of the culture plates obtained between each culture chamber (consistent height and consistent solidification time), thereby improving the reliability of the experimental results.

[0024] (3) By setting the partition parallel to the side wall of the dish and making the volume of each culture chamber formed by the partition equal, this utility model facilitates the formation of multiple culture spaces of the same size and shape that are independent of each other in the dish, thereby ensuring that the colony bands formed in the culture space are clear and do not cross each other, and further ensuring the reliability of the experimental results.

[0025] (4) By setting the distance between the top of the partition plate and the opening of the dish body to within 2 mm, preferably greater than zero and within 1 mm, this utility model ensures that the opening of the dish body is sealed by the cover body after the cover body is closed, thereby ensuring that the culture space inside the dish body is sealed during the experiment.

[0026] (5) By setting the height of the through hole to no more than one-third of the height inside the dish, or setting the height of the through hole to 2~10mm, the liquid level of the hot-melted culture medium poured into the dish can be higher than the highest point of the through hole, and the poured culture medium liquid can flow freely between different culture chambers through the through hole, thereby ensuring that the height of the culture medium in different culture spaces separated by the partition is equal after the culture medium solidifies, and the thickness of the culture medium after solidification is higher than the highest point of the through hole to form independent culture spaces, thereby ensuring that the formed colony bands are highly consistent, clear and without crossover, thus ensuring the reliability of the experimental results.

[0027] (6) This utility model provides 1 to 10 through holes on each of the partitions to facilitate the rapid flow and diffusion of the culture medium after heat melting.

[0028] (7) By setting the through holes on adjacent partitions in a corresponding manner (aligned setting), the present invention forms a channel for liquid flow, thereby avoiding flow obstruction caused by misalignment of through holes with liquid; at the same time, the uniform distribution of through holes on the same partition can also make the liquid flow smoother.

[0029] (8) The present invention uses the dish body and the partition inside it made of plastic or glass; usually disposable plastic material (e.g., polystyrene, i.e. PS) is used, which is convenient for industrial low-cost production and irradiation sterilization, etc., and glass material can also be used, which is convenient for high temperature and high pressure sterilization and reuse.

[0030] (9) By setting the partition and the dish body as an integral molding, this utility model avoids gaps at the connection between the partition and the dish body, thereby avoiding incomplete disinfection due to gap structure during disinfection. It also avoids the internal structure of the culture dish from detaching due to loose connection during movement or tilting, which would cause the cultured colony bands to cross-influence the experimental results.

[0031] In summary, this invention features a simple structure, low cost, and ease of use. When used in experiments to detect the titer of bactericidal antibodies, it not only increases the number of culture chambers, thereby improving experimental throughput, but also solves the problem of droplet path intersection during the tilting process of ordinary culture dishes. Furthermore, by setting through holes, it simplifies the operation steps of pouring in the culture solution after the solid culture medium has been heated and melted, improving experimental efficiency and playing a role in improving quality and efficiency, thus possessing higher practical value. Attached Figure Description

[0032] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0033] Figure 2 This is a top view of the dish body in this utility model;

[0034] Figure 3 This is a cross-sectional view of the dish body in the width direction of this utility model;

[0035] Among them, 1—plate, 2—lid, 3—partition, 4—culture chamber, 5—through hole. Detailed Implementation

[0036] The present invention will be further described in detail below with reference to the embodiments, but the implementation of the present invention is not limited thereto.

[0037] Example 1:

[0038] like Figures 1 to 3 As shown in the structure, this embodiment discloses a partitioned square culture dish, including a dish body 1 with an opening at the top and a cover body 2 for closing the opening. The dish body 1 is provided with several partitions 3. The partitions 3 are spaced apart in the dish body 1 and divide the dish body 1 into several elongated culture chambers 4. The bottom of each partition 3 is provided with a through hole 5 that connects the culture chambers 4 on both sides of the partition 3.

[0039] In this embodiment, both the dish body 1 and the cover body 2 are conventional configurations. The cover body 2 simply needs to be fitted to the opening of the dish body 1, which will not be described in detail here. The partition 3 is arranged at intervals inside the dish body 1 to divide the dish body 1 into several elongated culture chambers 4, thereby forming multiple culture spaces separated by the partition 3 on the solid culture medium (i.e., culture plate) solidified inside the dish body 1.

[0040] In this embodiment, the through hole 5 is mainly used to form a culture plate that meets the experimental requirements in the dish body 1, and the height of the culture plate is greater than the height of the through hole 5, so that independent cavities (i.e., culture spaces) separated by partitions 3 are formed on the culture plate for culturing colonies, thereby ensuring that the colony bands formed on the solid culture medium in the culture cavity 4 are clear and do not cross, thus ensuring the reliability of the experimental results.

[0041] In this embodiment, in order to form clear colony bands and ensure the reliability of experimental results, the partition 3 is arranged parallel to the side wall of the dish body 1, and the culture chambers 4 formed by the partition 3 are of equal volume, so that the volume of the solid culture medium solidified in each culture chamber 4 is equal, so as to ensure that each culture chamber 4 has a culture space of the same size, shape and volume for colony formation.

[0042] Furthermore, to ensure that the culture space inside dish 1 is sealed during the experiment, the top of the partition 3 is set to be flush with or slightly lower than the opening of dish 1, and the distance between the top of the partition 3 and the opening of dish 1 is within 2mm (the optimal distance is greater than zero and within 1mm).

[0043] Furthermore, to ensure the formation of culture spaces separated by partition 3 on the solid culture medium (i.e., culture plate), thereby ensuring the formation of clear, non-crossing colony bands, this embodiment employs the following method when setting the through-holes 5:

[0044] (1) The height of the through hole 5 shall not exceed one-third of the depth of the dish body 1.

[0045] The depth of commonly used bacterial culture dishes is generally 15 mm, while the depth of bacterial culture dishes for specific purposes can reach 20-30 mm. Therefore, the specific height of the through hole 5 can be determined by using culture dishes of different depths according to different experimental requirements. The depth of the dish body 1 can be adaptively designed according to experimental requirements, and the corresponding height of the through hole 5 is set in the range of 2-10 mm. In this embodiment, the height of the dish body 1 used is 15 mm, therefore, the height of the through hole 5 is set to 2-5 mm.

[0046] (2) The number of through holes 5 provided on each of the partitions 3 is 1 to 10.

[0047] The more through holes 5 are provided on the same partition 3, the smaller the width of the through holes 5 can be. Therefore, under normal circumstances, the number of through holes 5 on the same partition 3 should not exceed 10, and a more preferred choice is 2 to 5. In this embodiment, depending on the size of the dish 1 used, 2 or 3 through holes 5 are provided on the same partition 3, thereby avoiding the flow rate of the liquid culture medium being affected by the narrow width of the through holes 5.

[0048] (3) The through holes 5 on the adjacent partitions 3 are correspondingly arranged (aligned) to form a liquid flow channel.

[0049] In this embodiment, in order to ensure that the heated and melted liquid culture medium can flow quickly into each culture chamber 4 and reach a consistent liquid level after being poured into the dish 1, the through holes 5 located at corresponding positions on different partitions 3 are aligned and located on the same straight line, thereby avoiding too many bends in the liquid culture medium in the liquid flow channel, which would affect its flow speed.

[0050] (4) The through holes 5 on the same partition 3 are evenly distributed.

[0051] By configuring the through hole 5 as described above, it can be effectively ensured that the liquid culture medium after heat melting can cover the through hole 5. This ensures that the liquid culture medium can be poured into the dish body 1 at once to form the required culture plate, and also ensures that solid culture medium of the same thickness can be formed in each culture chamber 4 after condensation. Furthermore, the culture space above the solid culture medium in each culture chamber 4 is not interconnected with the culture space in its adjacent culture chamber 4, thereby effectively ensuring that the formed colony bands are not crossed and are clear, thus ensuring the reliability of the experimental results.

[0052] In this embodiment, although the shape of the through-hole 5 will affect the flow rate of the liquid culture medium to some extent, the degree of influence is limited. Therefore, circular, square, or other shapes of through-hole 5 are all acceptable. The larger the area of ​​the through-hole 5, the faster the flow rate of the liquid culture medium. In this embodiment, the shape of the partition 3 is the same as the shape of the longitudinal section of the dish body 1, which is square. Therefore, the through-hole 5 in this embodiment is square, so that the area of ​​the through-hole 5 obtained under the same setting conditions (such as the same height, width, and spacing) is larger, thereby enabling the liquid culture medium to achieve a greater flow rate.

[0053] Furthermore, to ensure that the liquid culture medium after heat melting can solidify in the dish 1 and form a solid culture medium that meets the experimental requirements, the dish 1 and the partition 3 are made of plastic or glass.

[0054] Furthermore, to ensure that there are no gaps at the connection between the partition 3 and the dish body 1, the partition 3 and the dish body 1 are integrally formed. This prevents the connection between the partition 3 and the dish body 1 from being too loose during subsequent connections, which could affect the experimental results. The integrally formed partition 3 and dish body 1 fundamentally eliminate the possibility of loose connections, thereby effectively and clearly forming bacterial colony bands and obtaining more reliable experimental results.

[0055] The working principle of this embodiment:

[0056] (1) The partition 3 forms a separation between each culture chamber 4, thereby ensuring that when the droplets dripped into the culture chamber 4 are tilted at a set angle, they can form a clear colony band that is evenly distributed and does not cross.

[0057] (2) By setting through holes 5 at the bottom or near the bottom of the partition plate 3, the liquid culture medium after heat melting can flow and spread between different culture chambers 4 through the through holes 5, thereby forming the same liquid level in each culture chamber 4, and then forming a solid culture medium of the same thickness in the culture chamber 4 after condensation.

[0058] It should be noted that the thickness of the solid culture medium formed should be greater than the height of the apex of the through hole 5, so as to prevent droplets in the culture chamber 4 from entering other culture chambers 4 through the through hole 5.

[0059] The usage method of this practical example is as follows:

[0060] Before use, the initial state of this embodiment is as follows: Figure 1 As shown, prepare the required mixture as instructed.

[0061] Detailed operation steps:

[0062] Step 1: Pour a sufficient amount of heated and melted liquid culture medium into one or more culture chambers 4 at once.

[0063] Step 2: Allow a certain amount of time for the liquid culture medium to enter other culture chambers 4 through the through hole 5 until the liquid culture medium is evenly distributed in each culture chamber 4 and reaches the same liquid level; if necessary, the process can be accelerated by slightly tilting or shaking the dish 1.

[0064] Step 3: Let the culture medium in dish 1 stand until it has completely cooled and solidified. Then, drop the prepared mixture into one end of culture chamber 4 one by one until all the prepared mixtures have been dropped into the corresponding culture chamber 4.

[0065] Step 4: Raise one side of the dish 1 into which the mixture is dropped. When the mixture in each culture chamber 4 flows along the direction of the culture chamber 4 to the other end of the culture chamber 4 and forms the required strip-shaped liquid flow, cover it with the lid 2 and mark the relevant experimental information (experiment start time, experimental group number, etc.).

[0066] Step 5: Based on the set incubation time, detect, record, and compare the number of surviving colonies in each culture chamber 4 until the set experimental target value is achieved.

[0067] After the experiment is completed, the above-mentioned disposable experimental equipment, such as dish 1 and lid 2, must be properly disposed of in accordance with the experimental requirements to avoid environmental pollution.

[0068] It should be noted that in step 2, the liquid level of the static liquid culture medium needs to be higher than the height of the through hole 5 to ensure that each culture chamber 4 is an independent space separated by the partition 3, thereby ensuring the accuracy of the experimental results.

[0069] In this embodiment, the colony count can be detected using an automatic counter or manual counting, which is a routine operation and will not be described in detail here.

[0070] In order to avoid the influence of external factors on the experimental results during the experiment, the dish body 1 (including the partition 3 inside) and the lid 2 can be sterilized. The sterilized dish body 1 and lid 2 are then sealed in a sterile container and taken out when needed. This effectively controls the aseptic process, avoids external influence on the experimental results, and improves the accuracy of the experimental results.

[0071] The compartmentalized square petri dish can be used to fill the required culture medium for culturing experimental subjects such as microorganisms (bacteria, fungi, viruses, etc.) and animal cells; the compartmentalized square petri dish can be used once or reused multiple times after sterilization.

[0072] The series of optimization schemes provided in this embodiment do not change the usage method of this utility model, and will not be described in detail here.

[0073] It should be noted that in this embodiment, the depth of the dish 1 refers to the vertical distance between the top of the side wall of the dish 1 and the bottom plane inside the dish 1; the height of the through hole 5 is the vertical distance between the highest point of the through hole 5 and the bottom plane inside the dish.

[0074] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Any simple modifications or equivalent changes made to the above embodiments based on the technical essence of the present utility model shall fall within the protection scope of the present utility model.

Claims

1. A compartmentalized square petri dish, characterized in that, It includes a dish body (1) with an opening at the top and a cover body (2) for closing the opening. The dish body (1) is provided with several partitions (3). The partitions (3) are spaced apart in the dish body (1) and divide the dish body (1) into several long strip-shaped culture chambers (4). Each partition (3) has a through hole (5) at the bottom or near the bottom that connects the culture chambers (4) on both sides of the partition (3).

2. The partitioned square petri dish according to claim 1, characterized in that, The partition (3) is arranged parallel to the side wall of the dish body (1), and the volumes of each culture chamber (4) formed by the partition (3) are equal.

3. The partitioned square petri dish according to claim 2, characterized in that, The spacing between adjacent partitions (3) is 5~25mm.

4. The partitioned square petri dish according to claim 1, characterized in that, The distance between the top of the partition (3) and the opening of the dish (1) is L, where 0≤L≤2mm.

5. The partitioned square petri dish according to claim 1, characterized in that, The height of the through hole (5) is no more than one-third of the depth of the dish body (1).

6. The partitioned square petri dish according to claim 5, characterized in that, The height of the through hole (5) is 2~10mm.

7. The partitioned square petri dish according to claim 1, characterized in that, The number of through holes (5) on each of the partitions (3) is 1 to 10.

8. The partitioned square petri dish according to claim 7, characterized in that, The through holes (5) on the same partition (3) are evenly distributed.

9. The partitioned square petri dish according to claim 1, characterized in that, The through holes (5) on adjacent partitions (3) are aligned to form a liquid flow channel.

10. The partitioned square petri dish according to claim 1, characterized in that, Both the dish body (1) and the partition (3) are made of plastic or glass.

11. The partitioned square petri dish according to any one of claims 1 to 10, characterized in that, The partition (3) is integrally formed with the dish body (1).

Images