Centrifugal tube for rapidly separating peripheral blood bone marrow mononuclear cells

Abstract

The utility model relates to the technical field of cell separation, and discloses a centrifugal tube for quickly separating peripheral blood bone marrow mononuclear cells, which comprises a centrifugal tube, an upper internal thread is arranged on the inner wall of the centrifugal tube, and a lower internal thread is arranged on the inner wall of the centrifugal tube. Blood and ficoll liquid are isolated through the filter membrane sheet and are prevented from being mixed too much to affect the separation effect, pure mononuclear cells can be simply, conveniently and rapidly collected in the centrifugal process due to the isolation effect of the inert separation gel and the blocking function of the filter membrane sheet, and after centrifugation is completed, the mononuclear cells are collected in a direct and rapid pouring mode, so that the separation effect is greatly improved. The inert separation gel is used for isolating centrifuged red blood cells, preventing the red blood cells from being mixed into separated mononuclear cells when supernatant is poured out, improving the separation method of the mononuclear cells, improving the separation efficiency of the mononuclear cells, reducing the experiment difficulty, saving the experiment time and guaranteeing the reliability of experiment results.

Description

Technical Field

[0001] This invention relates to the field of cell separation technology, and in particular to a centrifuge tube for rapidly separating peripheral blood bone marrow mononuclear cells. Background Technology

[0002] In the field of cell separation technology, traditional methods for isolating mononuclear cells mainly include density gradient centrifugation, primarily using Ficoll or Percoll solutions. However, these traditional methods require extreme care during the blood addition and mononuclear cell extraction processes, significantly increasing the difficulty and time consumption of the experiments. Furthermore, these methods carry the risk of red blood cell contamination in later stages and may result in incomplete mononuclear cell collection. Therefore, we propose a centrifuge tube for the rapid isolation of peripheral blood mononuclear cells (PBMCs). Utility Model Content

[0003] To solve the above-mentioned technical problems, this utility model provides a centrifuge tube for rapid separation of peripheral blood bone marrow mononuclear cells.

[0004] This utility model is achieved using the following technical solution: a centrifuge tube for rapidly separating peripheral blood bone marrow mononuclear cells, comprising a centrifuge tube, wherein the inner wall of the centrifuge tube is provided with an upper internal thread, the inner wall of the centrifuge tube is provided with a lower internal thread, the inner wall of the centrifuge tube is provided with a movable groove, the centrifuge tube is provided with a filter assembly inside, and the bottom of the inner wall of the centrifuge tube is provided with an inert separation gel.

[0005] The filter assembly includes a frame with external threads on its surface and a plurality of filter membranes fixedly connected to the inner wall of the frame.

[0006] The above technical solution isolates blood and Ficoll solution using a filter membrane to prevent excessive mixing that could affect the separation effect. During centrifugation, the inert separating gel and the filter membrane provide a simple and rapid way to collect pure mononuclear cells. After centrifugation, the mononuclear cells are collected by direct and rapid pouring. The inert separating gel isolates the centrifuged red blood cells, preventing them from mixing into the separated mononuclear cells when the supernatant is poured. This improves the mononuclear cell separation method, increases the separation efficiency, reduces experimental difficulty, saves experimental time, and ensures the reliability of the experimental results.

[0007] As a further improvement to the above solution, the external thread is adapted to the dimensions of the upper internal thread and the lower internal thread.

[0008] Through the above technical solution, the external thread can rotate inside the upper or lower internal thread, allowing the skeleton to move.

[0009] As a further improvement to the above scheme, small holes are formed between several of the filter membranes.

[0010] Using the above technical solution, several filter membranes are spliced ​​together to form a complete circle, with the small holes located in the middle of the filter membrane.

[0011] As a further improvement to the above solution, the skeleton is made of polypropylene and has a diameter of 15mm.

[0012] The above technical solution uses a frame to install and fix the filter membrane, ensuring stability during use.

[0013] As a further improvement to the above scheme, the pore size of the filter membrane is 20 μm.

[0014] With the above technical solution, since the filter membrane has a pore size of 20um, its function is to prevent too much mixing of blood and density gradient centrifugation liquid when blood is added rapidly, thus avoiding affecting the separation efficiency.

[0015] As a further improvement to the above solution, the diameter of the small hole is 0.3 mm.

[0016] The above technical solution allows for the easy addition of inert separating gel to the bottom of the centrifuge tube through a small hole.

[0017] As a further improvement to the above solution, the movable groove is located between the upper internal thread and the lower internal thread, and the length of the movable groove is 0.7cm.

[0018] With the above technical solution, the skeleton can be locked in place by the presence of the upper and lower internal threads, allowing the skeleton to move within the inner wall of the sliding groove. When there is a lot of blood, more inert separation gel can be added.

[0019] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0020] This invention improves the separation method of mononuclear cells by setting up centrifuge tubes and a filter assembly. Specifically, it uses a filter membrane to isolate blood and Ficoll solution, preventing excessive mixing of the two and affecting the separation effect. During centrifugation, due to the isolation effect of the inert separating gel and the blocking function of the filter membrane, pure mononuclear cells can be collected easily and quickly. After centrifugation, the mononuclear cells are collected by direct and rapid pouring. The inert separating gel isolates the centrifuged red blood cells, preventing them from mixing into the separated mononuclear cells when pouring the supernatant. This improves the separation method of mononuclear cells, increases the separation efficiency, reduces experimental difficulty, saves experimental time, and ensures the reliability of experimental results. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0022] Figure 2 This is a schematic cross-sectional view of the present invention.

[0023] Figure 3 This utility model Figure 2 Enlarged structural diagram of section A in the middle;

[0024] Figure 4 This is a schematic diagram of the filter assembly structure of this utility model;

[0025] Figure 5 This is a top view of the structure of this utility model.

[0026] Explanation of key symbols:

[0027] 1. Centrifuge tube; 2. Upper internal thread; 3. Lower internal thread; 4. Movable groove; 5. Filter assembly; 501. Frame; 502. External thread; 503. Filter membrane; 6. Small hole. Detailed Implementation

[0028] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0029] Example:

[0030] Please combine Figure 1-5 This embodiment of a centrifuge tube for rapid separation of peripheral blood bone marrow mononuclear cells includes a centrifuge tube 1, an upper internal thread 2 on the inner wall of the centrifuge tube 1, a lower internal thread 3 on the inner wall of the centrifuge tube 1, a movable groove 4 on the inner wall of the centrifuge tube 1, a filter assembly 5 inside the centrifuge tube 1, and an inert separation gel at the bottom of the inner wall of the centrifuge tube 1.

[0031] The filter assembly 5 includes a frame 501 with external threads 502 on its surface. Several filter membranes 503 are fixedly connected to the inner wall of the frame 501. The filter membranes 503 isolate blood and Ficoll solution, preventing them from mixing too much and affecting the separation effect. During centrifugation, due to the isolation effect of the inert separating gel and the blocking function of the filter membranes 503, pure mononuclear cells can be collected easily and quickly. Mononuclear cells are collected by direct and rapid pouring. The inert separating gel isolates the centrifuged red blood cells, preventing them from mixing into the separated mononuclear cells when pouring the supernatant. This improves the mononuclear cell separation method, increases the separation efficiency of mononuclear cells, reduces experimental difficulty, saves experimental time, and ensures the reliability of experimental results.

[0032] The external thread 502 is adapted to the size of the upper internal thread 2 and the lower internal thread 3. By rotating the skeleton 501, the external thread 502 can move inside the upper internal thread 2 or the lower internal thread 3.

[0033] Small holes 6 are formed between several filter membranes 503, which facilitate the addition of inert separating gel to the bottom of centrifuge tube 1.

[0034] The skeleton 501 is made of polypropylene and has a diameter of 15mm.

[0035] The pore size of filter membrane 503 is 20 μm.

[0036] The diameter of small hole 6 is 0.3mm.

[0037] The movable groove 4 is located between the upper internal thread 2 and the lower internal thread 3, and the length of the movable groove 4 is 0.7cm.

[0038] The implementation principle of a centrifuge tube for rapid separation of peripheral blood bone marrow mononuclear cells in this application embodiment is as follows: During use, inert separating gel is added to the bottom of the centrifuge tube 1 through the small hole 6. When blood is added to the inside of the centrifuge tube 1, the blood and Ficoll solution are isolated by the filter membrane 503 to prevent excessive mixing and thus maintain the separation effect. During centrifugation, due to the isolation effect of the inert separating gel and the blocking function of the filter membrane 503, pure mononuclear cells can be collected easily and quickly. After centrifugation, the mononuclear cells are collected by direct and rapid pouring. The inert separating gel isolates the centrifuged red blood cells, preventing them from mixing into the separated mononuclear cells when pouring the supernatant. This improves the mononuclear cell separation method, increases the separation efficiency, reduces experimental difficulty, saves experimental time, and ensures the reliability of experimental results.

[0039] The above embodiments are merely preferred embodiments of this utility model and should not be construed as limiting the scope of protection of this utility model. Any non-substantial changes and substitutions made by those skilled in the art based on this utility model shall fall within the scope of protection claimed by this utility model.

Claims

1. A centrifuge tube for rapidly separating peripheral blood bone marrow mononuclear cells, characterized in that, The centrifuge tube (1) is provided with an upper internal thread (2) on its inner wall, a lower internal thread (3) on its inner wall, a movable groove (4) on its inner wall, a filter assembly (5) inside the centrifuge tube (1), and an inert separating gel at the bottom of the inner wall of the centrifuge tube (1). The filter assembly (5) includes a frame (501), the surface of which is provided with external threads (502), and a plurality of filter membranes (503) are fixedly connected to the inner wall of the frame (501).

2. The centrifuge tube for rapid separation of peripheral blood bone marrow mononuclear cells as described in claim 1, characterized in that: The external thread (502) is adapted to the dimensions of the upper internal thread (2) and the lower internal thread (3).

3. The centrifuge tube for rapid separation of peripheral blood bone marrow mononuclear cells as described in claim 1, characterized in that: Small holes (6) are formed between several of the filter membrane sheets (503).

4. The centrifuge tube for rapid separation of peripheral blood bone marrow mononuclear cells as described in claim 1, characterized in that: The skeleton (501) is made of polypropylene and has a diameter of 15mm.

5. A centrifuge tube for rapid separation of peripheral blood bone marrow mononuclear cells as described in claim 3, characterized in that: The filter membrane (503) has a pore size of 20 μm.

6. The centrifuge tube for rapid separation of peripheral blood bone marrow mononuclear cells as described in claim 3, characterized in that: The diameter of the small hole (6) is 0.3 mm.

7. The centrifuge tube for rapid separation of peripheral blood bone marrow mononuclear cells as described in claim 1, characterized in that: The movable groove (4) is located between the upper internal thread (2) and the lower internal thread (3), and the length of the movable groove (4) is 0.7cm.

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