Accurate temperature control device of NAD + biosynthesis fermentation system

Abstract

The utility model relates to the technical field of bioengineering, and particularly discloses a precise temperature control device of an NAD + biosynthesis fermentation system, which comprises a water storage tank, a loopback conveying mechanism and a stirring mechanism, and a heat preservation mechanism formed by a heater and a heat preservation layer is arranged outside the water storage tank. The loopback conveying mechanism is composed of a loopback pipe rotationally connected to the top and the bottom of the inner side of the fermentation tank, circulating water is driven by a water pump to flow between the water storage tank and the loopback pipe to form closed-loop dynamic heat exchange, a first gear is fixed to the end of the loopback pipe, and a fixing plate is arranged on the outer side of a bottom connector; a driving motor, a rotating shaft and a second gear are mounted on the fixing plate, a loopback pipe is driven to rotate slowly through gear engagement, fermentation liquor is continuously disturbed, local temperature gradient is broken, liquid convection is enhanced in cooperation with a bottom stirring assembly, uniform distribution of temperature in the tank is achieved, a temperature sensor monitors and feeds back and adjusts water temperature in real time, and accurate temperature control is ensured; according to the device, through the synergistic effect of dynamic temperature control of circulating water and mechanical stirring, the efficiency of NAD < + > synthesis reaction and the product stability are remarkably improved, and meanwhile, the energy consumption is reduced.

Description

Technical Field

[0001] This utility model relates to the field of bioengineering technology, and more specifically, to a precise temperature control device for an NAD+ biosynthesis fermentation system. Background Technology

[0002] NAD+ (nicotinamide adenine dinucleotide) is a key coenzyme in cell metabolism and is widely used in biomedicine, food additives, and cosmetics. Traditional NAD+ biosynthesis mainly relies on microbial fermentation technology. However, insufficient temperature control precision and uneven temperature distribution within the fermentation vessel are the main problems restricting reaction efficiency and product stability during fermentation.

[0003] Existing technologies mostly use external heating or cooling devices for temperature control, but these have problems such as low heat transfer efficiency and lag in temperature response, making it difficult to meet the requirements of high-precision fermentation processes. In addition, although traditional stirring devices can improve temperature distribution, they consume a lot of energy and are prone to causing mechanical damage to microbial cells, affecting synthesis efficiency. Therefore, in order to address the above technical problems, a precise temperature control device for NAD+ biosynthesis fermentation system is proposed. Utility Model Content

[0004] The purpose of this invention is to provide a precise temperature control device for an NAD+ biosynthesis fermentation system. Through the synergistic effect of dynamic temperature control of circulating water and gear-driven rotation of the loop pipe, the temperature inside the fermenter is precisely controlled and local temperature differences are eliminated. The heater combined with the insulation layer efficiently maintains the water temperature. The loop pipe rotates slowly with the gear meshing, continuously agitating the fermentation broth and enhancing heat transfer. The bottom stirring component simultaneously enhances liquid convection, significantly improving the uniformity of temperature distribution. This optimizes NAD+ synthesis efficiency while reducing overall energy consumption.

[0005] This utility model is achieved through the following technical solution:

[0006] A precise temperature control device for an NAD+ biosynthesis fermentation system includes:

[0007] The water storage tank is installed outside the fermenter and is equipped with an external heat preservation mechanism.

[0008] A circular conveying mechanism is installed inside the fermenter, and a circulating conveying mechanism is installed inside the water storage tank.

[0009] The first gear is fixedly connected to the outside of one end of the loop pipe. A fixing plate is fixedly connected to the outside of the connector located near the bottom of the fermenter. An agitation mechanism is installed on the outside of the fixing plate.

[0010] Preferably, an inlet pipe is fixedly connected to the upper side of the fermentation tank, and an outlet pipe is fixedly connected to the lower side of the fermentation tank. Both the inlet pipe and the outlet pipe are threadedly connected to threaded caps at their ends.

[0011] Preferably, the water storage tank is externally fixedly connected to an inlet pipe, and the end of the inlet pipe is threadedly connected to a cap.

[0012] Preferably, the heat preservation mechanism includes a heater and a heat preservation layer. The heater is fixedly connected to the outside of the water storage tank. The heating part of the heater is located inside the water tank for heating, and the heat dissipation part of the heater is located outside the water tank for heat exchange. The heat preservation layer is laid on the outer surface of the water storage tank and completely covers the water storage tank.

[0013] Preferably, the loop conveying mechanism includes connectors and a loop pipe. There are two sets of connectors, which are fixedly connected to the top and bottom of the fermenter. The loop pipe is rotatably connected between the two sets of connectors and has a loop structure.

[0014] Preferably, the circulating conveying mechanism includes a water pump, an input pipe, and an output pipe. The input pipe is fixedly connected between the upper side of the water storage tank and the upper side of the fermentation tank. One end of the input pipe is located inside the water storage tank, and the other end of the input pipe passes through the outside of the fermentation tank and the connector and is located inside the connector. The output pipe is fixedly connected between the lower side of the water storage tank and the lower side of the fermentation tank. One end of the output pipe is located inside the water storage tank, and the other end of the output pipe passes through the outside of the fermentation tank and the connector and is located inside the connector. The water pump is fixedly connected to the inner side of the water storage tank, and the water pump and the input pipe are fixedly connected.

[0015] Preferably, the agitation mechanism includes a drive motor, a rotating shaft, and a second gear. The drive motor is fixedly connected to the outside of the fixed plate, the rotating shaft is fixedly connected to the upper side of the drive motor, and the second gear is fixedly connected to the outside of the rotating shaft, and the second gear meshes with the first gear.

[0016] Preferably, the inner bottom of the fermenter is fixedly connected to the mounting cylinder, and the connector and stirring mechanism located at the bottom are both located inside the mounting cylinder. The loop pipe and the mounting cylinder are rotatably connected, and a temperature sensor is fixedly connected to the inner side of the fermenter.

[0017] The technical solution of this utility model has at least the following beneficial effects:

[0018] The precise temperature control device of this NAD+ biosynthesis fermentation system significantly improves the temperature control accuracy and uniformity of the NAD+ biosynthesis fermentation process through the synergistic effect of dynamic temperature control of circulating water and gear-driven rotation of the loop pipe. Temperature sensors monitor the internal temperature of the fermenter in real time, while heaters and insulation layers efficiently regulate the temperature of the circulating water. Water pumps drive water flow to form a closed loop within the loop pipe, facilitating efficient heat exchange between the pipe wall and the fermentation broth, ensuring that the temperature inside the tank quickly stabilizes to the target range. Simultaneously, the loop pipe rotates slowly through gear meshing, continuously agitating the fermentation broth and breaking up local temperature gradients. Combined with the bottom stirring component, this further enhances liquid convection, achieving uniform temperature distribution within the tank. This not only improves the efficiency of the NAD+ synthesis reaction and the stability of the product but also significantly reduces energy consumption. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 2 for Figure 1 Enlarged view of A in the middle;

[0021] Figure 3 for Figure 1 Enlarged view of B in the middle;

[0022] Figure 4 for Figure 1 Enlarged view of C in the middle;

[0023] Icons: 1. Fermentation tank; 2. Feed pipe; 3. Discharge pipe; 4. Threaded cap; 5. Water storage tank; 6. Liquid inlet pipe; 7. Sealing cap; 8. Heater; 9. Insulation layer; 10. Connector; 11. Loop pipe; 12. Water pump; 13. Input pipe; 14. Output pipe; 15. First gear; 16. Fixing plate; 17. Drive motor; 18. Rotating shaft; 19. Second gear; 20. Mounting cylinder; 21. Temperature sensor. Detailed Implementation

[0024] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0025] Example:

[0026] Please see Figures 1-4This application proposes a precise temperature control device for an NAD+ biosynthesis fermentation system, comprising a water storage tank 5, a loop conveying mechanism, and a first gear 15 installed outside the fermenter 1. The water storage tank 5 is equipped with an insulation mechanism. The loop conveying mechanism is installed inside the fermenter 1, and a circulation conveying mechanism is installed inside the water storage tank 5. The first gear 15 is fixedly connected to the outside of one end of the loop pipe 11. A fixing plate 16 is fixedly connected to the outside of a connector 10 located near the bottom of the fermenter 1, and an agitation mechanism is installed on the outside of the fixing plate 16.

[0027] The upper side of the fermentation tank 1 is fixedly connected to the feed pipe 2, and the lower side of the fermentation tank 1 is fixedly connected to the discharge pipe 3. The ends of both the feed pipe 2 and the discharge pipe 3 are threaded with threaded caps 4. During fermentation, the threaded caps 4 on the outside of the feed pipe 2 are opened to allow the raw materials to be fed into the fermentation tank 1 for fermentation. After fermentation is complete, the threaded caps 4 at the end of the discharge pipe 3 can be unscrewed to discharge the raw materials.

[0028] The water storage tank 5 is externally fixedly connected to an inlet pipe 6, and the end of the inlet pipe 6 is threadedly connected to a cap 7. By opening the cap 7, circulating water or other heat exchange media can be injected into the water storage tank 5 to ensure the normal operation of the temperature control system.

[0029] The insulation mechanism includes a heater 8 and an insulation layer 9. The heater 8 is fixedly connected to the outside of the water storage tank 5. The heating part of the heater 8 is located inside the water tank for heating, and the heat dissipation part of the heater 8 is located outside the water tank for heat exchange. The insulation layer 9 is laid on the outer surface of the water storage tank 5 and completely wraps the water storage tank 5. Through the cooperation of the heater 8 and the insulation layer 9, heat loss is effectively reduced and temperature control efficiency is improved.

[0030] The loop conveying mechanism includes connectors 10 and loop pipes 11. There are two sets of connectors 10, which are fixedly connected to the top and bottom of the fermenter 1 respectively. The loop pipes 11 are rotatably connected between the two sets of connectors 10 and have a loop structure. Through the closed-loop flow of the loop pipes 11, the temperature inside the fermenter 1 can be efficiently controlled and evenly distributed.

[0031] The circulating conveying mechanism includes a water pump 12, an input pipe 13, and an output pipe 14. The input pipe 13 is fixedly connected between the upper side of the water storage tank 5 and the upper side of the fermentation tank 1. One end of the input pipe 13 is located inside the water storage tank 5, and the other end of the input pipe 13 passes through the fermentation tank 1 and the outside of the connector 10, located inside the connector 10. The output pipe 14 is fixedly connected between the lower side of the water storage tank 5 and the lower side of the fermentation tank 1. One end of the output pipe 14 is located inside the water storage tank 5, and the other end of the output pipe 14 passes through the fermentation tank 1 and the outside of the connector 10, located inside the connector 10. The water pump 12 is fixedly connected to the inner side of the water storage tank 5, and the water pump 12 is fixedly connected to the input pipe 13. The water pump 12 drives the circulating water to flow in the loop pipe 11, thereby realizing the dynamic adjustment of the temperature inside the fermentation tank 1.

[0032] The stirring mechanism includes a drive motor 17, a rotating shaft 18, and a second gear 19. The drive motor 17 is fixedly connected to the outside of the fixed plate 16, the rotating shaft 18 is fixedly connected to the upper side of the drive motor 17, and the second gear 19 is fixedly connected to the outside of the rotating shaft 18. The second gear 19 meshes with the first gear 15. The drive motor 17 drives the second gear 19 to rotate, which in turn drives the loop pipe 11 to rotate slowly, thereby achieving uniform stirring of the fermentation liquid.

[0033] The bottom inner side of the fermenter 1 is fixedly connected to the mounting cylinder 20, and the connector 10 and the stirring mechanism located at the bottom are both located inside the mounting cylinder 20. The loop pipe 11 is rotatably connected to the mounting cylinder 20. A temperature sensor 21 is fixedly connected to the inner side of the fermenter 1. The temperature sensor 21 monitors the temperature inside the fermenter 1 in real time to ensure the accurate operation of the temperature control system.

[0034] The working principle of the precise temperature control device for the NAD+ biosynthesis fermentation system based on the embodiment is as follows: This device integrates circulating temperature control and mechanical stirring functions to achieve high-precision temperature regulation and uniform distribution of the NAD+ biosynthesis fermentation system. After the device is started, the temperature sensor 21 collects the internal temperature data of the fermentation tank 1 in real time. If the temperature deviates from the set value, the heater 8 heats or dissipates heat to the circulating water in the water storage tank 5. At the same time, the insulation layer 9 wraps the water storage tank 5 to minimize heat loss. Then, the water pump 12 injects the heated water into the top connector 10 through the input pipe 13, which pushes the circulating water in the return pipe 11 to form a closed loop flow along the inner wall of the fermentation tank 1. The water undergoes efficient heat exchange with the fermentation liquid through the pipe wall. The low-temperature water then flows back to the water storage tank 5 through the bottom output pipe 14 for reheating, forming a dynamic temperature control cycle to ensure that the temperature inside the tank quickly stabilizes to the target range.

[0035] During this process, the first gear 15 fixed at the end of the loop pipe 11 is linked with the stirring mechanism. The drive motor 17 drives the rotating shaft 18 to rotate the second gear 19. After meshing with the first gear 15, the second gear 19 drives the loop pipe 11 to rotate slowly along the axial direction of the connector 10, causing the tube wall to disturb the fermentation liquid and break the local temperature gradient. At the same time, the stirring component at the bottom of the installation cylinder 20 further enhances the liquid convection. Combining the dual effects of the circulating heat transfer of the loop pipe 11 and the mechanical stirring, the temperature distribution inside the tank is made uniform, which significantly improves the efficiency of the NAD+ synthesis reaction and the stability of the product, while reducing energy consumption.

[0036] Although embodiments of this application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of this application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A precision temperature control device for NAD+ biosynthesis fermentation system, characterized in that, The utility model relates to a fermentation tank, which comprises a water storage tank (5) installed outside the fermentation tank (1), a heat preservation mechanism installed outside the water storage tank (5), a circulating conveying mechanism installed inside the fermentation tank (1), a first gear (15) fixedly connected to one end of the circulating pipe (11), a fixed plate (16) fixedly connected to the outside of the connector (10) near the bottom of the fermentation tank (1), and a stirring mechanism installed outside the fixed plate (16). A feeding pipe (2) is fixedly connected to the upper side of the fermentation tank (1), and a discharging pipe (3) is fixedly connected to the lower side of the fermentation tank (1). The ends of the feeding pipe (2) and the discharging pipe (3) are threadedly connected with screw caps (4). A liquid inlet pipe (6) is fixedly connected to the outside of the water storage tank (5), and the end of the liquid inlet pipe (6) is threadedly connected with a cover (7). The heat preservation mechanism comprises a heater (8) and a heat preservation layer (9). The heater (8) is fixedly connected to the outside of the water storage tank (5). The heating part of the heater (8) is located inside the water tank for heating, and the heat dissipation part of the heater (8) is located outside the water tank for heat exchange. The heat preservation layer (9) is laid on the outer surface of the water storage tank (5), and the heat preservation layer (9) wraps the water storage tank (5) on all sides.

2. The precision temperature control device for NAD+ biosynthesis fermentation system according to claim 1, wherein: The connector (10) is provided in two groups and is fixedly connected to the top and bottom of the inside of the fermentation tank (1). The circulating pipe (11) is rotatably connected between the two groups of connectors (10), and the circulating pipe (11) has a circulating structure.

3. The precision temperature control device for NAD+ biosynthesis fermentation system according to claim 1, wherein: The circulating conveying mechanism comprises a water pump (12), an input pipe (13), and an output pipe (14). The input pipe (13) is fixedly connected between the upper side of the water storage tank (5) and the upper side of the fermentation tank (1). One end of the input pipe (13) is located inside the water storage tank (5), and the other end of the input pipe (13) passes through the outside of the fermentation tank (1) and the connector (10) and is located inside the connector (10). The output pipe (14) is fixedly connected between the lower side of the water storage tank (5) and the lower side of the fermentation tank (1). One end of the output pipe (14) is located inside the water storage tank (5), and the other end of the output pipe (14) passes through the outside of the fermentation tank (1) and the connector (10) and is located inside the connector (10). The water pump (12) is fixedly connected to the inside of the water storage tank (5), and the water pump (12) is fixedly connected to the input pipe (13).

4. The precision temperature control device for NAD+ biosynthesis fermentation system according to claim 1, wherein: The stirring mechanism comprises a driving motor (17), a rotating shaft (18), and a second gear (19). The driving motor (17) is fixedly connected to the outside of the fixed plate (16). The rotating shaft (18) is fixedly connected to the upper side of the driving motor (17). The second gear (19) is fixedly connected to the outside of the rotating shaft (18), and the second gear (19) is engaged with the first gear (15).

5. The precision temperature control device for NAD+ biosynthesis fermentation system according to claim 1, wherein: ​ 6. The precision temperature control device for NAD+ biosynthesis fermentation system according to claim 5, wherein: ​ ​ 7. The precision temperature control device for NAD+ biosynthesis fermentation system according to claim 1, wherein: ​ 8. The precision temperature control device for NAD+ biosynthesis fermentation system according to claim 5, wherein: The inner bottom of the fermentation tank (1) is fixedly connected with a mounting cylinder (20), the connector (10) and the stirring mechanism located at the bottom are located inside the mounting cylinder (20), the loop pipe (11) is rotationally connected with the mounting cylinder (20), and the inner side of the fermentation tank (1) is fixedly connected with a temperature sensor (21).

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