Tumor cell culture device

Abstract

The utility model relates to the technical field of tumor cell culture devices, in particular to a tumor cell culture device which comprises a base and a heating plate, the bottom of the inner wall of the base is attached to the bottom of the heating plate, the top of the base is connected with a separation structure, and the top of the separation structure is connected with a plurality of control structures. The left side and the right side of the separation structure are connected with curved pipes correspondingly, and the tops of the curved pipes communicate with second valves. Quantitative carbon dioxide is fed into the partition structure through the gas pipeline, then the first valve is opened to enable the carbon dioxide and oxygen to enter the shell, and the partition plates in the shell divide the inner wall into four independent spaces; therefore, the tumor cells in each culture dish can be ensured to be in contact with quantitative 5% of carbon dioxide and oxygen, the use difficulty of the tumor cell culture device is reduced, and the normal use of the tumor cell culture device is ensured.

Description

Technical Field

[0001] This utility model relates to the technical field of tumor cell culture devices, specifically a tumor cell culture device. Background Technology

[0002] Tumor cell culture devices can be used for tumor diagnosis, prognostic assessment, and drug screening after tumor cells are cultured.

[0003] For example, a tumor cell culture device with publication number "CN119040125A" uses a heating coil to increase the heat at the bottom of the humidification box, allowing the clean water inside to evaporate and form tiny water vapor particles, increasing air humidity. A condenser plate cools the hot water vapor, lowering its temperature and allowing it to condense into water droplets for reuse, reducing waste. However, in the use of this tumor cell culture device, multiple culture dishes are in the same space. When operating on one dish, tumor cells in other dishes are exposed to a large area of ​​air. Furthermore, tumor cell culture requires 5% carbon dioxide, and controlling the carbon dioxide content in multiple dishes in the same space is difficult, increasing the difficulty of using the device and affecting its normal operation. Utility Model Content

[0004] The purpose of this invention is to solve the problem of increased difficulty in using tumor cell culture devices, which affects the normal use of tumor cell culture devices, and to propose a tumor cell culture device.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A tumor cell culture device is designed, including a base and a heating plate. The bottom of the inner wall of the base is attached to the bottom of the heating plate. A partition structure is connected to the top of the base. Multiple control structures are connected to the top of the partition structure. Curved tubes are connected to the left and right sides of the partition structure, and a second valve is connected to the top of the curved tubes.

[0007] Preferably, the partition structure includes an outer shell and a partition plate. The bottom of the outer shell is fixedly connected to the top of the base, the inner wall of the outer shell is fixedly connected to the end of the partition plate, and the front and rear sides of the outer shell are respectively connected to a first valve, and the outer side of the first valve is connected to a straight pipe.

[0008] Preferably, the bottom of the outer casing is attached to the top of the heating plate.

[0009] Preferably, the left and right sides of the outer shell are respectively connected to the inner sides of multiple curved tubes.

[0010] Preferably, the control structure includes a bracket and a transparent plate. The outer wall of the bracket is inserted into the top opening of the outer shell, the top opening of the bracket is fixedly connected to the outer wall of the transparent plate, a sleeve plate is fixedly connected to the top of the outer wall of the bracket, and the bottom of the sleeve plate is attached to the top of the outer shell.

[0011] Preferably, a handle is fixed to the rear top of the support, a temperature and humidity detector is fixed to the front round opening of the top of the support, and a culture dish is attached to the bottom of the inner wall of the support.

[0012] The tumor cell culture device proposed in this utility model has the following advantages: a fixed amount of carbon dioxide is introduced into the gas pipe through the partition structure, and then the first valve is opened to allow carbon dioxide and oxygen to enter the outer shell. Due to the partition inside the outer shell dividing the inner wall into four independent spaces, it can be ensured that the tumor cells in each culture dish are in contact with a fixed amount of 5% carbon dioxide and oxygen, which reduces the difficulty of using the tumor cell culture device and ensures the normal use of the tumor cell culture device. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of this utility model;

[0014] Figure 2 for Figure 1 A schematic diagram showing the connection structure between the central support, the transparent plate, and the sleeve plate;

[0015] Figure 3 for Figure 1 A schematic diagram showing the connection relationship between the outer shell, partition, and valves;

[0016] Figure 4 for Figure 1 A schematic diagram showing the connection structure between the base and the heating plate.

[0017] In the diagram: 1. Base, 2. Dividing structure, 201. Outer shell, 202. Partition plate, 203. First valve, 204. Straight pipe, 3. Control structure, 301. Support, 302. Transparent plate, 303. Sheet plate, 4. Heating plate, 5. Curved pipe, 6. Second valve, 7. Petri dish, 8. Temperature and humidity detector, 9. Handle. Detailed Implementation

[0018] The present invention will be further described below with reference to the accompanying drawings:

[0019] Please see Figure 1-4In this embodiment, a tumor cell culture device includes a base 1 and a heating plate 4. The bottom of the inner wall of the base 1 is attached to the bottom of the heating plate 4. A partition structure 2 is connected to the top of the base 1. Multiple control structures 3 are connected to the top of the partition structure 2. Curved tubes 5 are connected to the left and right sides of the partition structure 2 respectively. A second valve 6 is connected to the top of the curved tube 5. The second valve 6 is a one-way valve and the model is selected according to the usage requirements.

[0020] The partition structure 2 includes an outer shell 201 and a partition 202. The bottom of the outer shell 201 is fixedly connected to the top of the base 1. The inner wall of the outer shell 201 is fixedly connected to the end of the partition 202. The partition 202 forms four isolation spaces inside the outer shell 201. The front and rear sides of the outer shell 201 are respectively connected to a first valve 203. The first valve 203 is a one-way valve model selected according to the usage requirements. The outer side of the first valve 203 is connected to a straight pipe 204. The bottom of the outer shell 201 is attached to the top of the heating plate 4. The left and right sides of the outer shell 201 are respectively connected to the inner side of multiple curved pipes 5.

[0021] A fixed amount of carbon dioxide is introduced through the gas pipe in the partition structure 2. Then, the first valve 203 is opened to allow carbon dioxide and oxygen to enter the outer shell 201. Because the partition 202 inside the outer shell 201 divides the inner wall into four independent spaces, it can ensure that the tumor cells in each culture dish 7 are in contact with a fixed amount of five percent carbon dioxide and oxygen, which reduces the difficulty of using the tumor cell culture device and ensures the normal use of the tumor cell culture device.

[0022] The control structure 3 includes a support 301 and a transparent plate 302. The outer wall of the support 301 is inserted into the top opening of the outer shell 201. The top opening of the support 301 is fixed to the outer wall of the transparent plate 302. The transparent plate 302 is a glass plate. A sleeve 303 is fixed to the top of the outer wall of the support 301. The sleeve 303 is made of rubber. The bottom of the sleeve 303 is attached to the top of the outer shell 201. A handle 9 is fixed to the rear of the top of the support 301. The handle 9 makes it easy to pull the support 301 upward. A temperature and humidity detector 8 is fixed to the round opening at the front of the top of the support 301. The model of the temperature and humidity detector 8 is selected according to the usage requirements. A petri dish 7 is attached to the bottom of the inner wall of the support 301.

[0023] Working principle:

[0024] Tumor cell culture device for tumor cell placement:

[0025] Hold handle 9 and pull upwards. Handle 9 moves the transparent plate 302 upwards, and the moving transparent plate 302 moves the support 301 upwards through the top opening of the outer shell 201. At this time, the sleeve 303 detaches from the top of the outer shell 201 until the culture dish 7 inside the support 301 is exposed. Then, take out the culture dish 7 and place culture medium (to supply nutrients to the cells) and tumor cells on the inner wall of the culture dish 7. Then, put the culture dish 7 back into the support 301. Next, move handle 9 downwards so that the transparent plate 302 and the support 301 return to the outer shell 201 until the bottom of the sleeve 303 is in contact with the top of the outer shell 201. The sleeve 303 seals the shell with its own rubber material.

[0026] Use of tumor cell culture device:

[0027] The front flange of the straight pipe 204 is fixed to the external gas pipeline. Then, the second valve 6 is opened, and water is introduced into the curved pipe 5 through the second valve 6, allowing the water to enter the outer casing 201 through the curved pipe 5. Subsequently, the second valve 6 is closed, and the heating plate 4 is powered on (by properly controlling the direction and magnitude of the current, the semiconductor element can generate heat, thereby heating the constant temperature plate), maintaining the heating plate 4 between 35-38 degrees Celsius. At this time, the temperature and humidity detector 8 can be observed (the sensor in the temperature and humidity detector 8 senses the temperature and humidity information in the environment through resistance, capacitance, electromagnetic induction, etc., and converts it into an electrical signal, which is then converted into a digital signal by an internal analog-to-digital converter and displayed on the screen). When measuring the internal temperature of the outer shell 201, check if the humidity is below 70%. Then, introduce a fixed amount of carbon dioxide through the gas pipe. Next, open the first valve 203 to allow carbon dioxide and oxygen to enter the outer shell 201. Because the partition 202 inside the outer shell 201 divides the inner wall into four independent spaces, this ensures that the tumor cells in each culture dish 7 are in contact with a fixed amount of 5% carbon dioxide and oxygen. During this period, it is necessary to observe the inside of the support 301 through the transparent plate 302 to check if a yellow liquid is produced in the culture dish. If so, it means that the culture medium inside the culture dish needs to be replaced. At this time, follow the above operation to remove the culture dish 7 and replace the culture medium inside. Finally, put the culture dish 7 back into the outer shell 201.

[0028] After the tumor cell culture of this batch was completed, the stent 301 was removed from the shell 201, and then the inside of the shell 201 and the inside of the stent 301 were disinfected and sterilized respectively.

[0029] Although the present invention has been illustrated and described with reference to preferred embodiments, those skilled in the art should understand that various changes in form and detail are possible within the scope of the claims.

Claims

1. A tumor cell culture device comprising a base (1) and a heating plate (4), the inner wall bottom of the base (1) being in contact with the bottom of the heating plate (4), characterized in that: The top of the base (1) is connected with a separation structure (2), the top of the separation structure (2) is connected with a plurality of control structures (3), the left and right sides of the separation structure (2) are respectively connected with curved pipes (5), and the top of the curved pipe (5) is communicated with a second valve (6).

2. The tumor cell culture device of claim 1, wherein: The separation structure (2) comprises an outer shell (201) and a partition plate (202), the bottom of the outer shell (201) is fixedly connected with the top of the base (1), the inner wall of the outer shell (201) is fixedly connected with the end of the partition plate (202), the front and rear sides of the outer shell (201) are respectively communicated with first valves (203), and the outer side of the first valve (203) is communicated with a straight pipe (204).

3. The tumor cell culture device of claim 2, wherein: The bottom of the outer shell (201) is matched with the top of the heating plate (4).

4. The tumor cell culture device of claim 2, wherein: The left and right sides of the outer shell (201) are respectively communicated with the inner sides of the plurality of curved pipes (5).

5. The tumor cell culture device of claim 1, wherein: The control structure (3) comprises a support (301) and a transparent plate (302), the outer wall of the support (301) is inserted with the top opening of the outer shell (201), the top opening of the support (301) is fixedly connected with the outer wall of the transparent plate (302), the outer wall top of the support (301) is fixedly connected with a cover plate (303), and the bottom of the cover plate (303) is matched with the top of the outer shell (201).

6. The tumor cell culture device of claim 5, wherein: The top rear of the support (301) is fixedly connected with a handle (9), the top front round opening of the support (301) is fixedly connected with a temperature and humidity detector (8), and the inner wall bottom of the support (301) is matched with a culture dish (7).

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