Localizable fast-assembly Western Blot film rotating clamp system

Abstract

The utility model relates to the technical field of molecular biology experimental equipment, in particular to a locating and fast-assembling Western Blot film transferring clamp system which comprises a film transferring clamp base body, a buckle connecting piece capable of being fast connected is installed between the opening ends of the film transferring clamp base body, a sponge layer is arranged in the film transferring clamp base body, and a locating piece is arranged on the sponge layer. A first pressure-sensitive adhesive tape is bonded between the corner of the bottom of the sponge layer and the bottom of the transfer film clamp base body, filter paper is placed on the top of the sponge layer, a second pressure-sensitive adhesive tape is bonded between the corner of the top of the sponge layer and the bottom of the filter paper, and a polyvinylidene fluoride film is placed above the filter paper. Through a bonding, locking and scale cooperative positioning mechanism, the membrane transfer success rate and the experiment repeatability are remarkably improved, meanwhile, the operation complexity is reduced, and a reliable tool is provided for standardization of the western blot technology.

Description

Technical Field

[0001] This utility model relates to the field of molecular biology experimental equipment technology, specifically a positionable quick-release Western blotting membrane transfer clamp system. Background Technology

[0002] In recent years, Western blotting technology has been widely used in disease mechanism exploration and drug target verification as a key tool in molecular biology research. As the core consumable of this technology, the assembly accuracy and operation efficiency of the transfer clamp directly affect the transfer effect and experimental repeatability. Currently, the mainstream transfer clamp systems on the market generally have the following technical bottlenecks: (1) Difficulty in aligning multi-layer components: Traditional transfer clamps require manual alignment of filter paper, sponge and membrane components. Especially in the solvent-wetting environment, the filter paper is prone to sliding and shifting, resulting in misalignment of the transfer area and an increased failure rate of the experiment; (2) Insufficient reliability of the fixing method: Relying on simple buckles or bolts for locking, the locking force is easily reduced due to vibration or solvent swelling during long-term electro-transfer, which leads to an increase in the gap between components and uneven electric field distribution. Utility Model Content

[0003] The purpose of this invention is to provide a positionable and quick-release Western Blot transfer clamp system to solve the problems mentioned in the background art.

[0004] The technical solution of this utility model is: a positionable and quick-installation Western Blot transfer clamp system, including a transfer clamp base, with quick-connect snap fasteners installed between the open ends of the transfer clamp base, a sponge layer disposed inside the transfer clamp base, a first pressure-sensitive adhesive tape bonded between the bottom corner of the sponge layer and the bottom of the transfer clamp base, a filter paper placed on top of the sponge layer, a second pressure-sensitive adhesive tape bonded between the top corner of the sponge layer and the bottom of the filter paper, and a polyvinylidene fluoride membrane placed on top of the filter paper.

[0005] The aforementioned components achieve the following effects: the first pressure-sensitive adhesive tape fixes the filter paper to the inside of the transfer clamp substrate, and the second pressure-sensitive adhesive tape fixes the filter paper to the top of the sponge layer. Peelable pressure-sensitive adhesive strips are used for pre-fixation of the components between the transfer clamp substrate, the sponge layer, and the filter paper, avoiding manual alignment errors. Then, the SDS-PAGE gel is placed on the center mark of the filter paper before the polyvinylidene fluoride (PVDF) membrane is placed. Finally, snap-fit ​​connectors quickly lock the assembled transfer clamp substrate. Through the adhesive, locking, and scale-coordinated positioning mechanism, the success rate of transfer and experimental repeatability are significantly improved, while reducing operational complexity, providing a reliable tool for the standardization of protein blotting technology.

[0006] Preferably, the transfer clamp substrate includes a clamping piece one and a clamping piece two, with two flexible connectors fixed between the clamping piece one and the clamping piece two, and the snap-fit ​​connectors fixed to the inner side of the end of the clamping piece one and the clamping piece two away from the flexible connectors.

[0007] The effect achieved by the above components is that the positioning and clamping of the sponge layer, filter paper and polyvinylidene fluoride membrane can be completed by using clamping plate one and clamping plate two, flexible connector and snap-fit ​​connector.

[0008] Preferably, the flexible connector includes a connecting strap, and both ends of the connecting strap are fitted with connecting knobs that connect to the first clamp and the second clamp.

[0009] The above components achieve the following effect: the connecting strap and the connecting knob can fix the clip one and clip two together.

[0010] Preferably, the buckle connector includes a mushroom-shaped buckle fixed on the first clamp piece, the protruding end of the mushroom-shaped buckle having an elastic groove, and the second clamp piece having a buckle groove adapted to the mushroom-shaped buckle.

[0011] The effect achieved by the above components is that the mushroom-shaped buckle can be squeezed and deformed when the clip one and clip two are pressed and then inserted into the buckle groove, thus completing the quick and easy fixed connection of clip one and clip two.

[0012] Preferably, the number of mushroom head buckles is at least two, which are distributed in a straight line on the clip one, and the buckle groove corresponds to the number of mushroom head buckles.

[0013] The effect achieved by the above components is that the connection strength between clip one and clip two can be increased by multiple mushroom-head buckles and matching slots.

[0014] Preferably, the upper surface of the filter paper is provided with scale lines made of UV-cured waterproof ink.

[0015] The effect achieved by the above components is that the scale lines made of UV-cured waterproof ink can withstand the corrosion of solvents such as methanol.

[0016] This invention provides an improved, positionable, quick-release Western blotting transfer clamp system, which, compared with the prior art, has the following improvements and advantages:

[0017] This invention uses a first pressure-sensitive adhesive tape to fix the filter paper to the inside of the transfer clamp substrate, and then uses a second pressure-sensitive adhesive tape to fix the filter paper to the top of the sponge layer. A peelable pressure-sensitive adhesive strip is used for pre-fixation of the components between the transfer clamp substrate, the sponge layer, and the filter paper, avoiding manual alignment errors. Then, the SDS-PAGE gel is placed on the center mark of the filter paper before the polyvinylidene fluoride (PVDF) membrane is placed. Finally, a snap-fit ​​connector is used to quickly lock the assembled transfer clamp substrate. Through the adhesive, locking, and scale-coordinated positioning mechanism, the success rate of transfer and experimental repeatability are significantly improved, while reducing operational complexity, providing a reliable tool for the standardization of protein blotting technology. Attached Figure Description

[0018] The present invention will be further explained below with reference to the accompanying drawings and embodiments:

[0019] Figure 1 This is a cross-sectional structural schematic diagram of the present invention;

[0020] Figure 2 This is a schematic diagram of the unfolded structure of the transfer membrane clip substrate of this utility model;

[0021] Figure 3 This is a schematic diagram of the planar structure of the filter paper in this utility model.

[0022] Explanation of reference numerals in the attached figures:

[0023] 1. Transfer clamp substrate; 101. Clip 1; 102. Clip 2; 103. Connecting knob; 104. Connecting strap; 2. Mushroom head buckle; 3. Buckle groove; 4. Sponge layer; 5. First pressure-sensitive adhesive tape; 6. Second pressure-sensitive adhesive tape; 7. Filter paper; 8. Polyvinylidene fluoride membrane; 9. Scale lines. Detailed Implementation

[0024] The present invention will now be described in detail, and the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention.

[0025] This utility model provides an improved, positionable, quick-release Western blotting transfer clamp system. The technical solution of this utility model is as follows:

[0026] In embodiments of this utility model, such as Figures 1-3As shown, the positionable quick-release Western blotting transfer clamp system includes a transfer clamp base 1. The transfer clamp base 1 includes clamping piece 101 and clamping piece 102. Two flexible connectors are fixed between clamping piece 101 and clamping piece 102. A snap-fit ​​connector is fixed to the inner side of the end of clamping piece 101 and clamping piece 102 away from the flexible connector. Through clamping piece 101, clamping piece 102, and the flexible connectors in conjunction with the snap-fit ​​connector, the positioning and clamping of the sponge layer 4, filter paper 7, and polyvinylidene fluoride membrane 8 can be completed. The flexible connector includes a connecting strap 104, with connecting strips at both ends of the connecting strap 104 that connect to clamping piece 101 and clamping piece 102. The knob 103, via the connecting strap 104 and the connecting knob 103, enables the fixed connection between clamp 101 and clamp 102. A quick-connect snap-fit ​​connector is installed between the open ends of the transfer clamp base 1. The snap-fit ​​connector includes a mushroom-head snap-fit ​​2 fixed to clamp 101. The protruding end of the mushroom-head snap-fit ​​2 has an elastic groove. Clamp 102 has a snap-fit ​​groove 3 that matches the mushroom-head snap-fit ​​2. When clamp 101 and clamp 102 are pressed, the mushroom-head snap-fit ​​2 is squeezed and deformed, locking into the snap-fit ​​groove 3, thus completing the quick and secure connection between clamp 101 and clamp 102. At least two mushroom-head clips 2 are arranged in a straight line on clip 101. The number of clips 3 corresponds to the number of mushroom-head clips 2. The connection strength between clip 101 and clip 2 is increased by multiple mushroom-head clips 2 and matching clips 3. A sponge layer 4 is provided inside the transfer clamp base 1. A first pressure-sensitive adhesive tape 5 is bonded between the bottom corner of the sponge layer 4 and the bottom of the transfer clamp base 1. Filter paper 7 is placed on top of the sponge layer 4 and fixed to the inside of the transfer clamp base 1 by the first pressure-sensitive adhesive tape 5. Then, the filter paper 7 is fixed to the top of the sponge layer 4 by the second pressure-sensitive adhesive tape 6. The transfer clamp base 1, sponge... Layer 4 and filter paper 7 are pre-fixed using a peelable pressure-sensitive adhesive strip. A second pressure-sensitive adhesive tape 6 is bonded between the top corner of the sponge layer 4 and the bottom of the filter paper 7. The upper surface of the filter paper 7 is provided with scale lines 9 made of UV-cured waterproof ink. The scale lines 9 made of UV-cured waterproof ink can withstand the corrosion of solvents such as methanol. The scale lines 9 include a main scale (1cm) and a secondary scale (1mm). They are laser micro-engraved with a depth of 0.2mm. The engraved area is silanized to withstand the corrosion of solvents such as methanol and achieve precise alignment at the ±0.05mm level. A polyvinylidene fluoride membrane 8 is placed on top of the filter paper 7.

[0027] The working principle of the positioning quick-assembly Western Blot transfer clamp system provided by this utility model is as follows: The first pressure-sensitive adhesive tape 5 can be fixed inside the transfer clamp base 1, and then the filter paper 7 can be fixed on the top of the sponge layer 4 by the second pressure-sensitive adhesive tape 6. The transfer clamp base 1, the sponge layer 4 and the filter paper 7 are pre-fixed by peelable pressure-sensitive adhesive strips to avoid manual alignment errors. Then, the SDS-PAGE gel is placed on the center scale of the filter paper 7 and then the polyvinylidene fluoride membrane 8 (PVDF) is placed. Finally, the mushroom head buckle 2 can be squeezed and deformed when the clamp 1 101 and clamp 2 102 are pressed and locked into the buckle groove 3 to complete the quick fixing connection of clamp 1 101 and clamp 2 102. Through the adhesive, locking and scale collaborative positioning mechanism, the success rate of membrane transfer and experimental repeatability are significantly improved, while reducing the complexity of operation, providing a reliable tool for the standardization of protein blotting technology.

[0028] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A positionable quick-fit Western Blot membrane transfer clamp system comprising a membrane transfer clamp base (1), characterized in that: The opening end of the membrane transfer clamp base (1) is provided with a quick-connecting buckle connector, the sponge layer (4) is arranged in the membrane transfer clamp base (1), the first pressure-sensitive adhesive tape (5) is arranged between the bottom corner of the sponge layer (4) and the bottom of the membrane transfer clamp base (1), the filter paper (7) is placed on the top of the sponge layer (4), the second pressure-sensitive adhesive tape (6) is arranged between the top corner of the sponge layer (4) and the bottom of the filter paper (7), and the polyvinylidene fluoride membrane (8) is placed above the filter paper (7).

2. The positionable quick-fit Western Blot membrane transfer clamp system of claim 1, wherein: The membrane transfer clamp base (1) comprises a clamping piece one (101) and a clamping piece two (102), two flexible connectors are fixed between the clamping piece one (101) and the clamping piece two (102), and the buckle connector is fixed to the inner side of the clamping piece one (101) and the clamping piece two (102) away from the flexible connectors.

3. The positionable quick-fit Western Blot membrane transfer clamp system of claim 2, wherein: The flexible connector comprises a connecting belt (104), and the connecting belt (104) is sleeved with a connecting knob (103) connected with the clamping piece one (101) and the clamping piece two (102) at both ends.

4. The positionable quick-fit Western Blot membrane transfer clamp system of claim 2, wherein: The buckle connector comprises a mushroom head buckle (2) fixed on the clamping piece one (101), the protruding end of the mushroom head buckle (2) is provided with an elastic slot, and the clamping piece two (102) is provided with a buckle groove (3) matched with the mushroom head buckle (2).

5. The positionable quick-fit Western Blot membrane transfer clamp system of claim 4, wherein: The number of the mushroom head buckle (2) is at least two, which are arranged in a straight line on the clamping piece one (101), and the number of the buckle groove (3) corresponds to that of the mushroom head buckle (2).

6. The positionable quick-fit Western Blot membrane transfer clamp system of claim 1, wherein: The upper surface of the filter paper (7) is provided with scale lines (9) made of UV-cured waterproof ink.

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