Isothermal amplification device
By designing an isothermal amplification device that includes a heating pack and a placement mechanism, the problem of existing devices requiring power and water bath equipment is solved, realizing a convenient amplification process without external equipment and reducing costs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-03
- Publication Date
- 2026-04-07
AI Technical Summary
Existing isothermal amplification devices require power supplies and small water baths, which affects the convenience of on-site testing.
An isothermal amplification device was designed, comprising a chamber, a heating pack, a temperature sensor, and a display screen. The heating pack generates heat by reacting with water, the temperature is controlled by the temperature sensor, and the test tubes are fixed by a placement mechanism, thus realizing an amplification process without the need for an external power source.
It enables on-site amplification without the need for external equipment, improving the convenience of testing and reducing costs.
Smart Images
Figure CN224091858U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of experimental instrument technology, specifically to an isothermal amplification device. Background Technology
[0002] Isothermal amplification is a relatively new nucleic acid amplification method that is simple, rapid, and highly specific. Compared with conventional PCR, it does not require template thermal denaturation, temperature cycling, electrophoresis, or ultraviolet observation. This technology can rival or even surpass PCR in terms of sensitivity, specificity, and detection range. It can achieve high-throughput rapid detection on-site without relying on any specialized instruments or equipment, and the detection cost is far lower than that of quantitative real-time PCR.
[0003] However, existing isothermal amplification devices still have shortcomings. Specifically, existing isothermal amplification devices also require power supplies and small water bath equipment, which greatly reduces the convenience of on-site testing.
[0004] Therefore, an isothermal amplification device is needed to solve the problems mentioned in the background art. Utility Model Content
[0005] The purpose of this invention is to provide an isothermal amplification device to solve the problems mentioned in the background art.
[0006] To achieve the above objectives, this utility model provides the following technical solution:
[0007] An isothermal amplification device includes a housing, an internal placement mechanism, a heating pack located inside the housing and below the placement mechanism, a temperature sensor fixedly connected inside the housing and at the connection point to the heating pack, a display screen mounted on the front of the housing, a lid connected to the top of the housing via a hinge, and a battery installed inside the housing.
[0008] The placement mechanism includes a test tube rack slidably connected inside the box body. The test tube rack has a test tube slot inside. A guide rod is slidably connected inside the test tube rack near the test tube slot. A support plate is fixedly connected to the bottom end of the guide rod below the test tube rack. A return spring is fixedly connected to the outer wall of the guide rod above the support plate. A locking block is slidably connected inside the test tube rack near the guide rod. An inner spring is fixedly connected to the outer wall of the locking block inside the test tube rack. A slot is formed inside the guide rod above the test tube rack. A push plate is slidably connected to the top of the locking block. An adjusting ring is fixedly connected to the top of the push plate above the test tube rack. An outer spring is fixedly connected to the bottom of the adjusting ring. A buffer pad is fixedly connected to the top of the support plate.
[0009] As a preferred embodiment of this utility model, both the box body and the box lid are made of polypropylene plastic, and the outer walls of both the box body and the box lid are coated with thermal insulation coating.
[0010] As a preferred embodiment of this utility model, the heating pack is a food self-heating pack, and the temperature sensor, display screen and battery are all connected electrically.
[0011] As a preferred embodiment of this utility model, the card block and the push plate are both made of aluminum alloy, and the reset spring, inner spring and outer spring are all fixedly connected to the test tube rack.
[0012] As a preferred embodiment of this utility model, the guide rod extends through and beyond the guide rod, and the connection between the push plate and the test tube rack is a sliding connection.
[0013] As a preferred embodiment of this utility model, the tray has a U-shaped structure design, and the shape of the slot is adapted to the shape of the card block.
[0014] As a preferred embodiment of this utility model, the pusher plate has a trapezoidal structure design, and the test tube groove has three different diameter specifications.
[0015] As a preferred embodiment of this utility model, the test tube rack, guide rod, tray, and adjusting ring are all made of polypropylene plastic, and multiple sets of the test tube groove, guide rod, tray, and locking block are provided.
[0016] As a preferred embodiment of this utility model, the push plate passes through the card block and extends to the outside of the test tube rack, and the guide rod has a T-shaped structure design.
[0017] As a preferred embodiment of this utility model, the cushioning pad is made of silicone, and the shape of the cushioning pad is adapted to the shape of the tray.
[0018] Compared with the prior art, the beneficial effects of this utility model are:
[0019] 1. In this utility model, an isothermal amplification device is designed. A placement mechanism within the device is used to hold test tubes. The chamber lid is opened, the test tube rack is removed, and water is injected into the chamber. A heating pack reacts with the water to generate a large amount of heat, thereby heating the water inside the chamber. A temperature sensor detects the temperature inside the chamber and displays the temperature data on a screen. When the internal temperature of the chamber reaches Chel... After DNA extraction, LAMP, and RPA reactions at the required temperatures, the test tube rack is placed back into the chamber. Then, the test tube containing the sample is inserted into the test tube slot. The test tube falls onto the tray. Pushing the test tube moves the tray and guide rod downwards. When the slot on the guide rod aligns with the locking block, an inner spring pushes the locking block outwards. The outward-moving locking block inserts into the slot, locking the guide rod and thus fixing the test tube in the test tube slot. The sample in the test tube begins amplification. During the amplification process, no power supply or other equipment is required, reducing the dependence on equipment for on-site testing. This solves the problem that existing isothermal amplification devices still require power supplies and small water baths, significantly reducing the convenience of on-site testing. Attached Figure Description
[0020] Figure 1 This is a three-dimensional structural diagram of the present invention;
[0021] Figure 2 This is a front sectional view of the housing of this utility model;
[0022] Figure 3 This utility model Figure 2 Enlarged view of point A in the middle.
[0023] In the diagram: 1. Box body; 2. Placement mechanism; 3. Heating pack; 4. Temperature sensor; 5. Display screen; 6. Box cover; 7. Battery; 201. Test tube rack; 202. Test tube trough; 203. Guide rod; 204. Support plate; 205. Return spring; 206. Locking block; 207. Inner spring; 208. Locking slot; 209. Push plate; 210. Adjusting ring; 211. Outer spring; 212. Buffer pad. Detailed Implementation
[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.
[0025] To facilitate understanding of this utility model, a more comprehensive description of the utility model will be given below with reference to the accompanying drawings, and several embodiments of the utility model will be provided. However, the utility model can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the utility model more thorough and complete.
[0026] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.
[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of this invention is for the purpose of describing particular embodiments only and is not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.
[0028] For examples, please refer to Figures 1-3 This utility model provides a technical solution:
[0029] An isothermal amplification device includes a housing 1, a placement mechanism 2 inside the housing 1, a heating pack 3 inside the housing 1 and below the placement mechanism 2, a temperature sensor 4 fixedly connected inside the housing 1 and at the location where the heating pack 3 is connected, a display screen 5 installed on the front of the housing 1, a housing cover 6 connected to the top of the housing 1 by a hinge, and a storage battery 7 installed inside the housing 1.
[0030] The box body 1 and the box cover 6 are both made of polypropylene plastic. The outer walls of the box body 1 and the box cover 6 are coated with heat insulation coating. The heating pack 3 is a food self-heating pack. The temperature sensor 4, the display screen 5 and the storage battery 7 are all connected by electrical connection.
[0031] In this embodiment, reference Figure 2 and Figure 3The placement mechanism 2 includes a test tube rack 201 slidably connected inside the housing 1. The test tube rack 201 has a test tube slot 202 inside. A guide rod 203 is slidably connected inside the test tube rack 201 near the test tube slot 202. A support plate 204 is fixedly connected to the bottom end of the guide rod 203 below the test tube rack 201. A return spring 205 is fixedly connected to the outer wall of the guide rod 203 above the support plate 204. A return spring 205 is fixedly connected inside the test tube rack 201 near the guide rod 203. A locking block 206 is slidably connected to the placement, and an inner spring 207 is fixedly connected to the outer wall of the locking block 206 and inside the test tube rack 201. A slot 208 is opened inside the guide rod 203 and above the test tube rack 201. A push plate 209 is slidably connected to the top of the locking block 206. An adjusting ring 210 is fixedly connected to the top of the push plate 209 and above the test tube rack 201. An outer spring 211 is fixedly connected to the bottom of the adjusting ring 210. A buffer pad 212 is fixedly connected to the top of the support plate 204.
[0032] The locking block 206 and the push plate 209 are both made of aluminum alloy. The return spring 205, inner spring 207, and outer spring 211 are all fixedly connected to the test tube rack 201. The guide rod 203 extends through and beyond the guide rod 206. The push plate 209 is slidably connected to the test tube rack 201. The support plate 204 has a U-shaped structure design. The shape of the slot 208 matches the shape of the locking block 206. The push plate 209 has a trapezoidal structure design. The test tube slot 202 has three different diameter specifications. The test tube rack 201, guide rod 203, support plate 204, and adjusting ring 210 are all made of polypropylene plastic. The test tube rack 201 is constructed with multiple sets of components, including test tube holder 202, guide rod 203, tray 204, and locking block 206. A push plate 209 passes through the locking block 206 and extends to the outside of the test tube rack 201. The guide rod 203 has a T-shaped structure. The buffer pad 212 is made of silicone, and its shape matches the shape of the tray 204. The lid 6 is opened, the test tube rack 201 is removed, and clean water is injected into the chamber 1. The heating pack 3 reacts with the clean water to generate a large amount of heat, thereby heating the water inside the chamber 1. The temperature sensor 4 detects the temperature inside the chamber 1 and displays the temperature data on the display screen 5. When the internal temperature of the chamber 1 reaches a certain level... After DNA extraction using the LE EX-100 and the necessary temperatures for LAMP and RPA reactions, the test tube rack 201 is placed back into the housing 1. Then, the test tube containing the sample is inserted into the test tube slot 202, and the test tube falls onto the tray 204. Pushing the test tube causes the tray 204 and guide rod 203 to move downwards. When the slot 208 on the guide rod 203 aligns with the locking block 206, the inner spring 207 pushes the locking block 206 outwards. The outwardly moving locking block 206 inserts into the slot 208, locking the guide rod 203, thereby fixing the test tube in the test tube slot 202. The sample in the test tube begins to amplify upon heating.
[0033] The working process of this utility model is as follows: When using the isothermal amplification device designed in this scheme, open the box cover 6, take out the test tube rack 201, and inject clean water into the box 1. The heating pack 3 reacts with the clean water to generate a large amount of heat, thereby heating the clean water in the box 1. The temperature sensor 4 detects the temperature inside the box 1 and displays the temperature data on the display screen 5. When the internal temperature of the box 1 reaches the temperature required for Chelex-100 DNA extraction, LAMP and RPA reactions, put the test tube rack 201 back into the box 1, and then insert the test tube containing the sample into the test tube slot 202. The test tube will fall onto the tray 204. Pushing the test tube will cause the tray 204 and guide rod 203 to move downward. When the slot 208 on the guide rod 203 aligns with the locking block 206, the inner spring 207 pushes the locking block 206 outward. The outwardly moving locking block 206 will insert into the slot 208, and the locking block 206 will lock the guide. The guide rod 203 is used to fix the test tube in the test tube slot 202. The sample in the test tube is heated and begins to amplify. After amplification, the adjusting ring 210 is pressed, which drives the push plate 209 to move downward. The descending push plate 209 pushes the locking block 206 to move inward. The locking block 206 moves inward and exits the slot 208. The locking block 206 no longer locks the guide rod 203. The reset spring 205 drives the support plate 204 and the test tube to move upward. The test tube is pushed out of the test tube rack 201 and the user takes out the test tube.
[0034] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. An isothermal amplification device, comprising a housing (1), characterized in that: The box (1) is provided with a placement mechanism (2) inside. A heating pack (3) is provided inside the box (1) and below the placement mechanism (2). A temperature sensor (4) is fixedly connected inside the box (1) and at the position where the heating pack (3) is connected. A display screen (5) is installed on the front of the box (1). A box cover (6) is connected to the top of the box (1) by a hinge. A storage battery (7) is installed inside the box (1). The placement mechanism (2) includes a test tube rack (201) slidably connected inside the housing (1). The test tube rack (201) has a test tube slot (202) inside. A guide rod (203) is slidably connected inside the test tube rack (201) near the test tube slot (202). A support plate (204) is fixedly connected to the bottom end of the guide rod (203) below the test tube rack (201). A return spring (205) is fixedly connected to the outer wall of the guide rod (203) above the support plate (204). A return spring (205) is fixedly connected inside the test tube rack (201) near the guide rod (203). A locking block (206) is slidably connected to the test tube rack (201). An inner spring (207) is fixedly connected to the outer wall of the locking block (206) and inside the test tube rack (201). A slot (208) is opened inside the guide rod (203) and above the test tube rack (201). A push plate (209) is slidably connected to the top of the locking block (206). An adjusting ring (210) is fixedly connected to the top of the push plate (209) and above the test tube rack (201). An outer spring (211) is fixedly connected to the bottom of the adjusting ring (210). A buffer pad (212) is fixedly connected to the top of the support plate (204).
2. The isothermal amplification device according to claim 1, characterized in that: The box body (1) and the box cover (6) are both made of polypropylene plastic, and the outer walls of the box body (1) and the box cover (6) are coated with heat insulation coating.
3. The isothermal amplification device according to claim 1, characterized in that: The heating pack (3) is a food self-heating pack, and the temperature sensor (4), display screen (5) and battery (7) are all electrically connected.
4. The isothermal amplification device according to claim 1, characterized in that: The card block (206) and the push plate (209) are both made of aluminum alloy. The reset spring (205), the inner spring (207) and the outer spring (211) are all fixedly connected to the test tube rack (201).
5. The isothermal amplification device according to claim 1, characterized in that: The guide rod (203) extends through and beyond the guide rod (203), and the push plate (209) is connected to the test tube rack (201) by a sliding connection.
6. The isothermal amplification device according to claim 1, characterized in that: The tray (204) has a U-shaped structure design, and the shape of the slot (208) is adapted to the shape of the block (206).
7. The isothermal amplification device according to claim 1, characterized in that: The push plate (209) has a trapezoidal structure design, and the test tube trough (202) has three different diameter specifications.
8. The isothermal amplification device according to claim 1, characterized in that: The test tube rack (201), guide rod (203), tray (204) and adjusting ring (210) are all made of polypropylene plastic, and multiple sets of test tube slots (202), guide rods (203), trays (204) and locking blocks (206) are provided.
9. The isothermal amplification device according to claim 1, characterized in that: The push plate (209) passes through the card block (206) and extends to the outside of the test tube rack (201), and the guide rod (203) has a T-shaped structure design.
10. The isothermal amplification device according to claim 1, characterized in that: The cushioning pad (212) is made of silicone and its shape is adapted to the shape of the tray (204).