Plasma free DNA enrichment device built in blood vessel

Abstract

The utility model discloses a blood vessel built-in plasma free DNA enrichment device, which comprises a probe body, a clamping groove, an assembly structure and a rubber plug, through the arrangement of the assembly structure, a worker pushes two groups of push plates inwards to enable two groups of movable structures to slide towards the inner side so as to extrude two groups of first springs, so that the two groups of first springs are separated from each other; when the probe body is assembled, the first spring contracts and drives the two limiting structures to move at the same time, after the movable structure moves in place, limiting on the probe body is relieved, the clamping block is separated from the clamping groove, then the probe body can be separated from the assembly structure, and the effect of conveniently disassembling and assembling the probe body and the assembly structure is achieved; through standardized design, replacement of probe bodies with different magnetic bead amounts and probe sizes is facilitated, and mutual replacement of an assembly structure and probe bodies produced by different manufacturers is facilitated.

Description

Technical Field

[0001] This invention relates to the field of cell-free DNA, and more particularly to an intravascular plasma cell-free DNA enrichment device. Background Technology

[0002] Cell-free DNA extraction can be used for purposes such as prenatal paternity testing and prenatal non-invasive DNA testing. Prenatal paternity testing refers to the use of genetic technology to identify the genetic father of the fetus. When the genetic father of the fetus is unknown, prenatal paternity testing can extract fetal DNA from the amniotic fluid or venous blood of the pregnant woman and the fetal villi, and confirm the kinship by identifying the fetal DNA.

[0003] CN208617837U discloses a device for enriching circulating cell-free DNA, comprising a probe, multiple magnetic beads, and connecting tubes. The probe includes a straight tube, a conical block, and multiple connecting tubes. The straight tube is a hollow cylinder open at both ends, with an annular cavity formed inside. Multiple filter holes are evenly distributed on the wall of the straight tube, with one end of the filter hole connected to the outside and the other end connected to the annular cavity. The conical block has a hollow internal structure and is coaxially arranged with the straight tube, and the conical block is connected to the annular cavity. The diameter of the conical block increases along the direction close to the straight tube. One end of the connecting tube is connected to the straight tube, and the other end passes through the outer wall of the conical block. Multiple connecting tubes are evenly distributed around the circumference of the conical block. Multiple magnetic beads are evenly embedded in the annular cavity. One end of the connecting tube is connected to the conical block. The probe and magnetic beads are placed into a central vein to directly enrich cell-free DNA in the vein without the need to draw blood, thus avoiding the impact of cell rupture on cell-free DNA enrichment after blood extraction.

[0004] Although the aforementioned device for enriching circulating cell-free DNA has certain advantages in terms of enrichment capabilities, it lacks a standardized design. For example, parameters such as the amount of magnetic beads and the size of the probe may need to be adjusted according to different application scenarios, making it inconvenient to interchange components produced by different manufacturers. Utility Model Content

[0005] Therefore, in order to overcome the above-mentioned shortcomings, this utility model provides a blood vessel-embedded plasma free DNA enrichment device.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a blood vessel-embedded plasma free DNA enrichment device, comprising a probe body, slots, an assembly structure, and a rubber stopper. Slots are provided on both the left and right sides of the upper end face of the probe body, and the slots are connected to the assembly structure. A rubber stopper is provided at the upper end of the assembly structure. The assembly structure includes a mounting plate, a first sliding groove, a second sliding groove, a third sliding groove, a movable structure, and a first spring. The first sliding groove is provided on both the left and right sides of the upper end face of the mounting plate. A second sliding groove is provided on the lower end face of the first sliding groove, and a third sliding groove is provided on the lower end face of the second sliding groove. The inner wall of the third sliding groove is slidably engaged with the movable structure. The inner wall of the movable structure is elastically connected to the inner wall of the second sliding groove via the first spring. The lower end face of the mounting plate is in contact with the probe body.

[0007] Optionally, the movable structure includes a rectangular plate, a push plate, a first sliding plate, a movable plate, a second sliding plate, a moving plate, and a limiting structure. The push plate is installed on the upper end face of the rectangular plate, the first sliding plate is installed on the lower end face of the rectangular plate, the movable plate is installed on the lower end face of the first sliding plate, the second sliding plate is installed on the lower end face of the movable plate, the moving plate is installed on the lower end face of the second sliding plate, a limiting structure is provided on the lower side of the moving plate, and the inner sidewall of the movable plate is connected to a first spring.

[0008] Optionally, the limiting structure includes a rectangular groove, a limiting block, a second spring, and a locking block. The limiting block is provided inside the rectangular groove. One end of the limiting block is elastically connected to the inner wall of the rectangular groove through the second spring. The other end of the limiting block is equipped with a locking block, which extends out of the moving plate. The rectangular groove is opened on the lower inner side of the moving plate.

[0009] Optionally, a sealing ring is provided at the center of the upper end face of the probe body.

[0010] Optionally, the two sets of active structures are symmetrically distributed.

[0011] Optionally, the first sliding plate, the movable plate, and the second sliding plate are slidably engaged with the first sliding groove, the second sliding groove, and the third sliding groove, respectively.

[0012] Optionally, the lower end face of the card block is inclined.

[0013] The beneficial effects of this utility model are:

[0014] This invention relates to an intravascular plasma-free DNA enrichment device. Through an assembly structure, operators push two sets of push plates inward, causing two sets of movable structures to slide inward, thereby compressing two sets of first springs. This causes the first springs to contract, simultaneously moving two sets of limiting structures. Once the movable structures are in place, they release the limiting effect on the probe body, and the locking block separates from the slot, thus separating the probe body from the assembly structure. This facilitates easy assembly and disassembly of the probe body and assembly structure. The standardized design allows for the replacement of probe bodies with different magnetic bead dosages and probe sizes, and facilitates the interchangeability of the assembly structure with probe bodies from different manufacturers. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the assembly structure of this utility model;

[0017] Figure 3 This is a three-dimensional schematic diagram of the movable structure of this utility model;

[0018] Figure 4 This is a top view of the movable structure of this utility model;

[0019] Figure 5 This is a schematic diagram of the limiting structure of this utility model.

[0020] The components include: probe body-1, slot-2, assembly structure-3, rubber plug-4, mounting plate-31, first sliding groove-32, second sliding groove-33, third sliding groove-34, movable structure-35, first spring-36, rectangular plate-351, push plate-352, first sliding plate-353, movable plate-354, second sliding plate-355, moving plate-356, limiting structure-357, rectangular groove-3571, limiting block-3572, second spring-3573, and locking block-3574. Detailed Implementation

[0021] To further explain the technical solution of this utility model, a detailed description is provided below through specific embodiments.

[0022] Please see Figure 1-2This invention provides an intravascular plasma free DNA enrichment device, comprising a probe body 1, slots 2, an assembly structure 3, and a rubber stopper 4. Slots 2 are formed on both the left and right sides of the upper end face of the probe body 1, and the slots 2 are connected to the assembly structure 3. A rubber stopper 4 is provided at the upper end of the assembly structure 3. The assembly structure 3 includes a mounting plate 31, a first sliding groove 32, a second sliding groove 33, a third sliding groove 34, a movable structure 35, and a first spring 36. The first sliding groove 32 is formed on both the left and right sides of the upper end face of the mounting plate 31, and a second sliding groove 33 is formed on the lower end face of the first sliding groove 32. Furthermore, a third sliding groove 34 is provided on the lower end face of the second sliding groove 33. The inner wall of the third sliding groove 34 is slidably engaged with the movable structure 35. The inner side wall of the movable structure 35 is elastically connected to the inner wall of the second sliding groove 33 through the first spring 36. The lower end face of the mounting plate 31 is attached to the probe body 1. A sealing ring is provided in the middle of the upper end face of the probe body 1 to prevent gaps between the probe body 1 and the mounting plate 3. The two sets of movable structures 35 are symmetrically distributed to facilitate better clamping and limiting of the probe body 1. The probe body 1 includes magnetic beads, which enrich free DNA through the magnetism of the magnetic beads.

[0023] Please see Figure 3-4 This invention provides an intravascular plasma free DNA enrichment device. The movable structure 35 includes a rectangular plate 351, a push plate 352, a first sliding plate 353, a movable plate 354, a second sliding plate 355, a moving plate 356, and a limiting structure 357. The push plate 352 is installed on the upper end face of the rectangular plate 351, the first sliding plate 353 is installed on the lower end face of the rectangular plate 351, the movable plate 354 is installed on the lower end face of the first sliding plate 353, the second sliding plate 355 is installed on the lower end face of the movable plate 354, and the moving plate 356 is installed on the lower end face of the second sliding plate 355. The limiting structure 357 is provided on the lower side of the moving plate 356. The inner sidewall of the movable plate 354 is connected to a first spring 36. The first sliding plate 353, the movable plate 354, and the second sliding plate 355 slide in cooperation with the first sliding groove 32, the second sliding groove 33, and the third sliding groove 34, respectively, which facilitates the smooth left-right sliding of the movable structure 35 as a whole.

[0024] Please see Figure 5This utility model provides an intravascular plasma free DNA enrichment device. The limiting structure 357 includes a rectangular groove 3571, a limiting block 3572, a second spring 3573, and a locking block 3574. The limiting block 3572 is disposed inside the rectangular groove 3571. One end of the limiting block 3572 is elastically connected to the inner wall of the rectangular groove 3571 through the second spring 3573. The locking block 3574 is installed at the other end of the limiting block 3572 and extends out of the moving plate 356. The rectangular groove 3571 is opened on the lower side inside the moving plate 356. The lower end face of the locking block 3574 is inclined to facilitate the rapid installation of the probe body 1 and the assembly structure 3.

[0025] The working principle is as follows:

[0026] First, the probe body 1 of the new device is embedded in the central vein, and free DNA is enriched by the magnetism of the magnetic beads. After enrichment, the probe body 1 is removed for subsequent processing.

[0027] Secondly, by simultaneously pushing two sets of push plates 352, the two sets of push plates 352 drive the rectangular plate 351 to move, the rectangular plate 351 drives the first sliding plate 353 to move, the first sliding plate 353 drives the movable plate 354 to move, the movable plate 354 drives the second sliding plate 355 to move, and the second sliding plate 355 drives the movable plate 356 to move. At the same time, the first sliding plate 353, the movable plate 354, and the second sliding plate 355 slide along the inner walls of the first sliding groove 32, the second sliding groove 33, and the third sliding groove 34, respectively. The movable plate 354 compresses the first spring 36, causing the first spring 36 to contract. At this time, the limiting of the locking block 3574 is released, making it easy to separate the locking block 3574 from the locking groove 2, thereby separating the probe body 1 and the assembly structure 3. Through standardized design, it is convenient to replace probe bodies with different magnetic bead amounts and probe sizes, and it is convenient for the assembly structure to be interchanged with probe bodies 1 produced by different manufacturers.

[0028] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A blood vessel-embedded plasma-free DNA enrichment device, characterized in that: The assembly includes a probe body (1), a slot (2), an assembly structure (3), and a rubber plug (4). The probe body (1) has slots (2) on both the left and right sides of its upper surface, which are connected to the assembly structure (3). The assembly structure (3) has a rubber plug (4) at its upper end. The assembly structure (3) includes a mounting plate (31), a first sliding groove (32), a second sliding groove (33), a third sliding groove (34), a movable structure (35), and a first spring (36). The upper end face of the 1) is provided with a first sliding groove (32) on both the left and right sides. The lower end face of the first sliding groove (32) is provided with a second sliding groove (33), and the lower end face of the second sliding groove (33) is provided with a third sliding groove (34). The inner wall of the third sliding groove (34) is slidably engaged with the movable structure (35). The inner side wall of the movable structure (35) is elastically connected to the inner wall of the second sliding groove (33) through a first spring (36). The lower end face of the mounting plate (31) is attached to the probe body (1).

2. The intravascular plasma-free DNA enrichment device according to claim 1, characterized in that: The movable structure (35) includes a rectangular plate (351), a push plate (352), a first sliding plate (353), a movable plate (354), a second sliding plate (355), a moving plate (356), and a limiting structure (357). The push plate (352) is installed on the upper end surface of the rectangular plate (351), the first sliding plate (353) is installed on the lower end surface of the rectangular plate (351), the movable plate (354) is installed on the lower end surface of the first sliding plate (353), the second sliding plate (355) is installed on the lower end surface of the movable plate (354), the moving plate (356) is installed on the lower end surface of the second sliding plate (355), and a limiting structure (357) is provided on the lower side of the moving plate (356). The inner sidewall of the movable plate (354) is connected to the first spring (36).

3. The intravascular plasma-free DNA enrichment device according to claim 2, characterized in that: The limiting structure (357) includes a rectangular groove (3571), a limiting block (3572), a second spring (3573), and a locking block (3574). The limiting block (3572) is provided inside the rectangular groove (3571). One end of the limiting block (3572) is elastically connected to the inner wall of the rectangular groove (3571) through the second spring (3573). The other end of the limiting block (3572) is equipped with a locking block (3574), and the locking block (3574) extends out of the moving plate (356). The rectangular groove (3571) is opened on the lower inside of the moving plate (356).

4. The intravascular plasma-free DNA enrichment device according to claim 1, characterized in that: A sealing ring is provided at the middle of the upper end face of the probe body (1).

5. The intravascular plasma-free DNA enrichment device according to claim 1, characterized in that: The two sets of active structures (35) are symmetrically distributed.

6. The intravascular plasma cell-free DNA enrichment device according to claim 2, characterized in that: The first sliding plate (353), the movable plate (354), and the second sliding plate (355) are respectively slidably engaged with the first sliding groove (32), the second sliding groove (33), and the third sliding groove (34).

7. The intravascular plasma-free DNA enrichment device according to claim 3, characterized in that: The lower end face of the card block (3574) is inclined.

Images