Mesenchymal stem cell culture device for arthritis treatment

Abstract

The utility model discloses a mesenchymal stem cell culture device for arthritis treatment, which comprises a culture box and a mesenchymal stem cell culture temperature adjusting mechanism, and a mesenchymal stem cell separation adjusting mechanism capable of rotating and moving is arranged above the inner wall of the culture box. The mesenchymal stem cell culture temperature adjusting mechanism is fixedly mounted on one side of the outer wall of the culture box, a culture solution replacing mechanism for mounting is arranged below the outer wall of the culture box, and a feeding pipe is connected and mounted on one side of the inner wall of the culture box. According to the mesenchymal stem cell culture device for treating arthritis, the mesenchymal stem cell separation and adjustment mechanism is arranged, so that mesenchymal stem cells can be separated, and when the mesenchymal stem cells are cultured, the mesenchymal stem cells can be compared; the mesenchymal stem cells are placed from the inner wall of the feeding pipe, so that the mesenchymal stem cells can be uniformly placed.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of arthritis, concretely is a kind of mesenchymal stem cell treatment culture device for arthritis treatment. BACKGROUND

[0002] Arthritis refers to the inflammation, infection, degeneration, trauma or other factors caused by inflammation in the joints and its surrounding tissues, which can be divided into dozens of kinds. There are more than 100 million arthritis patients in China, and the number is increasing. According to statistics, half of the population over 50 years old in China suffers from osteoarthritis, and 90% of women and 80% of men over 65 years old suffer from osteoarthritis. The prevalence rate in China is 0.34%-0.36%, and the life expectancy of severe patients is shortened by 10-15 years. At present, mesenchymal stem cells are often used in the treatment of arthritis, and therefore culture devices are often used for comparative observation of mesenchymal stem cells during research.

[0003] The mesenchymal stem cell culture device currently used is not convenient for comparing mesenchymal stem cells during culture, and the mesenchymal stem cell culture solution cannot be directly replaced. UTILITY MODEL CONTENT

[0004] The utility model aims to provide a kind of mesenchymal stem cell treatment culture device for arthritis treatment, to solve the problem of the mesenchymal stem cell culture device currently used in the above background technology, which is not convenient for comparing mesenchymal stem cells during culture, and the mesenchymal stem cell culture solution cannot be directly replaced.

[0005] To achieve the above purpose, the utility model provides the following technical scheme: a kind of mesenchymal stem cell treatment culture device for arthritis treatment, including incubator and mesenchymal stem cell culture temperature adjusting mechanism, the inner wall of the incubator is provided with rotatable movable mesenchymal stem cell separation adjusting mechanism, the mesenchymal stem cell culture temperature adjusting mechanism is fixedly installed on the outer wall of incubator one side, the outer wall of the incubator is provided with culture solution replacement mechanism for installation below, the inner wall of the incubator is connected and installed with feed pipe, the inner wall of the incubator is provided with permeable membrane for installation below.

[0006] Preferably, the mesenchymal stem cell separation adjusting mechanism includes a fixed ring, a shaft, a sleeve ring and a separation plate, the fixed ring is movably connected to the inner wall of the incubator below, the inner wall of the fixed ring is provided with a rotatable shaft, the outer surface of the shaft is provided with a sleeve ring with matching size, and the outer wall of the sleeve ring is connected and installed with a separation plate.

[0007] Preferably, the sleeve is rotatably connected to the fixing ring through a rotating shaft, and the outer wall of the rotating shaft is tightly attached to the inner wall of the sleeve.

[0008] Preferably, the mesenchymal stem cell culture temperature adjusting mechanism comprises a heater, a connecting block, an air outlet and barrier material, the heater is fixedly installed on one side of the outer wall of the incubator, the connecting block is fixedly installed on one side of the inner wall of the incubator, an air outlet is formed on the outer wall of the connecting block, and the inner surface of the air outlet is provided with the barrier material for installation.

[0009] Preferably, the culture solution replacing mechanism comprises a discharging port, a filter screen, a cover plate and a buckle, the discharging port is formed in the lower portion of the outer wall of the incubator, the lower portion of the inner surface of the discharging port is provided with the filter screen for installation, the lower surface of the discharging port is provided with the cover plate matched in size, and the cover plate is connected to the discharging port through the buckle.

[0010] Preferably, the buckle is connected to the incubator through the discharging port, and the outer wall of the buckle is tightly attached to the inner wall of the discharging port.

[0011] Compared with the prior art, the mesenchymal stem cell culture device for treating arthritis has the following beneficial effects:

[0012] 1. The mesenchymal stem cell culture device for treating arthritis is provided with a mesenchymal stem cell separation adjusting mechanism, so that the mesenchymal stem cells can be separated, compared and placed uniformly.

[0013] 2. The mesenchymal stem cell culture device for treating arthritis is provided with a mesenchymal stem cell culture temperature adjusting mechanism, so that the temperature for culturing the mesenchymal stem cells can be slowly adjusted to control the living environment of the mesenchymal stem cells within a certain range, and a culture solution replacing mechanism is arranged, so that the culture solution for culturing the mesenchymal stem cells can be conveniently replaced. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a front view structural schematic diagram of the utility model;

[0015] Figure 2 It is a mesenchymal stem cell separation adjusting mechanism structural schematic diagram of the utility model;

[0016] Figure 3 It is a mesenchymal stem cell culture temperature adjusting mechanism structural schematic diagram of the utility model;

[0017] Figure 4The utility model discloses culture solution replacement mechanism structure schematic diagram.

[0018] In the drawing: 1, incubator;2, mesenchymal stem cell separation adjusting mechanism;201, fixed ring;202, pivot;203, sleeve ring;204, separation board;3, mesenchymal stem cell culture temperature adjusting mechanism;301, heater;302, connecting block;303, gas outlet;304, barrier material;4, culture solution replacement mechanism;401, blanking port;402, filter screen;403, apron;404, buckle;5, feed pipe;6, osmotic membrane. DETAILED DESCRIPTION

[0019] The technical scheme in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative work belong to the protection scope of the utility model.

[0020] Please refer to Figures 1-4The utility model provides a technical scheme: a mesenchymal stem cell culture device for arthritis treatment, including incubator 1 and mesenchymal stem cell culture temperature adjusting mechanism 3, the upper side of the inner wall of incubator 1 is equipped with rotatable movable mesenchymal stem cell separation adjusting mechanism 2, mesenchymal stem cell separation adjusting mechanism 2 includes fixed ring 201, pivot 202, collar 203 and partition plate 204, the inner wall lower side of incubator 1 is movably connected with fixed ring 201, the inner wall center of fixed ring 201 is equipped with rotatable pivot 202, the outer surface of pivot 202 is equipped with the collar 203 of size matching, the outer wall one side of collar 203 is connected with the installation of partition plate 204, and rotating collar 203 makes it drive partition plate 204 to rotate, so this can adjust the interval between fixed ring 201, and the angle formed by partition plate 204 is adjusted, so that the mesenchymal stem cells can be separated, which can be compared and cultured for mesenchymal stem cells, and the mesenchymal stem cells are placed into the inner wall of feed pipe 5 into the inner surface position of fixed ring 201, and rotating pivot 202 makes it drive fixed ring 201 to rotate, so that the mesenchymal stem cells can be placed in each position of fixed ring 201, and the position of the mesenchymal stem cells can be stirred, so that they can be evenly distributed on the position of fixed ring 201, preventing the accumulation of mesenchymal stem cells, and collar 203 forms a rotating structure with fixed ring 201 through pivot 202, and the outer wall of pivot 202 is closely attached to the inner wall of collar 203, and collar 203 is rotatably connected to the surface position of pivot 202 in an up-down structure, so that the position of collar 203 on fixed ring 201 can be adjusted, so that the two collars 203 can determine the area of the mesenchymal stem cell growth environment;

[0021] Mesenchymal stem cell culture temperature adjusting mechanism 3 is fixedly installed on the outer wall of incubator 1, and mesenchymal stem cell culture temperature adjusting mechanism 3 includes a heater 301, a connecting block 302, an air outlet 303 and a barrier material 304, the heater 301 is fixedly installed on the outer wall of the incubator 1, and the connecting block 302 is fixedly installed on the inner wall of the incubator 1, the air outlet 303 is formed on the outer wall of the connecting block 302, and the barrier material 304 is arranged on the inner surface of the air outlet 303, the power of the heater 301 is turned on, so that the hot air can be discharged from the air outlet 303 installed on the outer wall of the connecting block 302, and the hot air can slowly flow through the inner wall of the barrier material 304, so that the environment of the mesenchymal stem cells during culture can be changed, so that the mesenchymal stem cells can be directly and stably controlled within a certain range;

[0022] The outer wall of the incubator 1 is provided with a culture solution replacement mechanism 4 for installation, which comprises a discharge port 401, a filter screen 402, a cover plate 403 and a buckle 404. The discharge port 401 is arranged on the lower side of the outer wall of the incubator 1, and the filter screen 402 is arranged on the inner surface of the discharge port 401. The cover plate 403 is arranged on the lower surface of the discharge port 401, and the buckle 404 is connected to the discharge port 401. The buckle 404 is arranged on the outer wall of the cover plate 403, and the outer wall of the buckle 404 is arranged on the two sides of the outer wall of the discharge port 401. In this way, the mesenchymal stem cell culture solution can be replaced from the lower side of the filter screen 402, and the mesenchymal stem cells can be isolated on the lower side of the fixing ring 201. The buckle 404 is connected to the incubator 1 through the discharge port 401, and the outer wall of the buckle 404 is tightly attached to the inner wall of the discharge port 401. In this way, the culture solution can be directly placed on the cover plate 403, so that the culture solution can be directly placed on the lower side of the mesenchymal stem cells.

[0023] The inner wall of the incubator 1 is provided with an inlet pipe 5, and the lower side of the inner wall of the incubator 1 is provided with a permeable membrane 6.

[0024] Working principle: first, place the device in the designated position, rotate the collar 203 to drive the partition plate 204 to rotate, so as to adjust the interval between the fixing rings 201, so as to separate the mesenchymal stem cells, so as to compare the culture of the mesenchymal stem cells, place the mesenchymal stem cells from the inner wall of the inlet pipe 5 into the inner surface of the fixing ring 201, rotate the shaft 202 to drive the fixing ring 201 to rotate, so as to place the mesenchymal stem cells on each position of the fixing ring 201, prevent the accumulation of mesenchymal stem cells, and the culture solution can fall into the inner surface of the discharge port 401 through the permeable membrane 6. The buckle 404 arranged on the outer wall of the cover plate 403 is arranged on the two sides of the outer wall of the discharge port 401. In this way, the mesenchymal stem cell culture solution can be replaced from the lower side of the filter screen 402, and the mesenchymal stem cells can be isolated on the lower side of the fixing ring 201. Turn on the power of the heater 301, so that the hot air can be discharged from the air outlet 303 arranged on the outer wall of the connecting block 302, and the hot air can slowly flow through the inner wall of the blocking material 304, so as to change the environment of the mesenchymal stem cells during culture. In this way, the mesenchymal stem cell culture can be directly and stably controlled within a certain range, so as to complete the operation process of the mesenchymal stem cell culture device for treating arthritis.

[0025] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A mesenchymal stem cell culture device for the treatment of arthritis, characterized in that, The system includes a culture chamber (1) and a mesenchymal stem cell culture temperature regulation mechanism (3). The upper part of the inner wall of the culture chamber (1) is provided with a rotatable mesenchymal stem cell separation regulation mechanism (2). The mesenchymal stem cell culture temperature regulation mechanism (3) is fixedly installed on one side of the outer wall of the culture chamber (1). The lower part of the outer wall of the culture chamber (1) is provided with a culture medium replacement mechanism (4) for installation. The inner wall of the culture chamber (1) is connected to and installed with a feed pipe (5). The lower part of the inner wall of the culture chamber (1) is provided with a permeable membrane (6) for installation.

2. The mesenchymal stem cell culture device for arthritis treatment according to claim 1, characterized in that: The mesenchymal stem cell separation and adjustment mechanism (2) includes a fixed ring (201), a rotating shaft (202), a collar (203), and a separator (204). The fixed ring (201) is movably connected to the lower inner wall of the incubator (1). The center of the inner wall of the fixed ring (201) is provided with a rotatable rotating shaft (202). The outer surface of the rotating shaft (202) is provided with a collar (203) of a matching size. The separator (204) is connected and installed on one side of the outer wall of the collar (203).

3. The mesenchymal stem cell culture device for arthritis treatment according to claim 2, characterized in that: The collar (203) forms a rotating structure with the fixed ring (201) through the rotating shaft (202), and the outer wall of the rotating shaft (202) is closely fitted with the inner wall of the collar (203). The collar (203) is rotatably connected to the surface position of the rotating shaft (202) in an up-down structure.

4. The mesenchymal stem cell culture device for treating arthritis according to claim 1, characterized in that: The mesenchymal stem cell culture temperature regulation mechanism (3) includes a heater (301), a connecting block (302), an air outlet (303), and a barrier material (304). The heater (301) is fixedly installed on one side of the outer wall of the culture box (1). The connecting block (302) is fixedly installed on one side of the inner wall of the culture box (1). An air outlet (303) is provided on the upper side of the outer wall of the connecting block (302). The inner surface of the air outlet (303) is provided with a barrier material (304) for installation.

5. A mesenchymal stem cell culture device for treating arthritis according to claim 1, characterized in that: The culture medium replacement mechanism (4) includes a discharge port (401), a filter screen (402), a cover plate (403), and a buckle (404). The discharge port (401) is located below the outer wall of the incubator (1). A filter screen (402) for installation is provided below the inner surface of the discharge port (401). A cover plate (403) of matching size is provided on the lower surface of the discharge port (401). The cover plate (403) is connected to the discharge port (401) by the buckle (404).

6. A mesenchymal stem cell culture device for treating arthritis according to claim 5, characterized in that: The buckle (404) is engaged with the incubator (1) through the discharge port (401), and the outer wall of the buckle (404) is tightly fitted with the inner wall of the discharge port (401).

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