Exosome culture equipment

Abstract

The utility model relates to the technical field of exosome culture, and discloses exosome culture equipment which comprises a culture chamber, a partition plate is fixedly mounted in the culture chamber, a sealing cover is fixedly mounted at the top of the culture chamber, a sample outlet hole is formed in the partition plate, a bearing table is rotatably mounted in the culture chamber, and a sample outlet hole is formed in the bearing table. A plurality of bearing columns are slidably mounted at the top of the bearing table, culture dishes are arranged at the tops of the bearing columns, and the outer side walls of the bearing columns are attached to the inner walls of the sample outlet holes; in the process of producing exosomes through cell culture, when a series of operations such as observation of culture dishes are needed, the selected culture dishes are rotated to the position under the sample outlet holes by rotating the bearing table, then the bearing columns are lifted in a sliding mode, so that the culture dishes extend out of the sample outlet holes, and the outer side walls of the bearing columns are attached to the inner walls of the sample outlet holes; when the sealing cover is opened, the culture room is still in a separated state from the outside, and the external environment cannot influence the environment in the culture room.

Description

TECHNICAL FIELD

[0001] The utility model relates to exosome culture technical field especially relates to a kind of exosome culture equipment. BACKGROUND

[0002] Exosome is the biological active material of lipid, protein and nucleic acid etc. that cell secretes naturally, is the vesicle of 30-150nm in diameter, as stem cell plays the important way of biological effect, compared with stem cell has low immunogenicity, high stability, nanometer can pass through blood-brain barrier, high activity, not easy to tumorigenicity etc. advantage, through a variety of mechanisms such as target cell binding, endocytosis, membrane fusion, and then donor cell expression information is transmitted to receptor cell, target cell reprogramming and play biological regulation function, it has been confirmed that exosome has the mechanism characteristics such as regulating inflammatory response, promoting cell proliferation migration, promoting angiogenesis and regulating matrix reconstruction.

[0003] The existing exosome culture equipment needs to observe the cell culture dish during the exosome culture process, and the existing exosome culture equipment is mostly a unified culture chamber. When the culture equipment is opened, the culture chamber exchanges substances with the outside world, which may introduce microbial contamination, cause evaporation of the culture medium and fluctuation of the gas concentration, and interfere with cell metabolism. UTILITY MODEL CONTENT

[0004] The utility model aims at providing an exosome culture equipment to solve the above-mentioned deficiencies in the prior art.

[0005] Technical scheme: an exosome culture equipment, comprising a culture chamber, a partition plate is fixedly installed inside the culture chamber, a sealing cover is fixedly installed on the top of the culture chamber, a sample outlet is formed in the partition plate, a bearing table is rotatably installed inside the culture chamber, a plurality of bearing columns are slidably installed on the top of the bearing table, a plurality of culture dishes are arranged on the top of the bearing columns, and the outer wall of the bearing column is matched with the inner wall of the sample outlet.

[0006] Further description of the above technical scheme: a motor is fixedly installed on the bottom of the culture chamber, a gear is fixedly installed on the output end of the motor and penetrates the bottom of the culture chamber, a gear ring is fixedly installed on the bottom of the bearing table, the gear ring is engaged with the gear, and the gear and the gear ring are both rotatably installed on the bottom of the culture chamber.

[0007] Further description of the above technical scheme: a sliding block is fixedly installed on the bottom side wall of the bearing column, the sliding block penetrates the inner wall of the bearing table and is slidably matched with the bearing table, a gas cylinder is fixedly installed on the bottom of the culture chamber, an adapter block is fixedly installed on the output end of the gas cylinder and penetrates the bottom of the culture chamber, and the sliding block is connected with the adapter block.

[0008] As the further description of the above technical scheme: the top of the inner wall of the sample outlet is fixedly installed with a rubber ring, and an expansion opening is formed in the bottom of the sample outlet.

[0009] As the further description of the above technical scheme: the bottom of the sealing cover is fixedly installed with a filling block, and the filling block is attached to the top of the culture chamber and the partition plate.

[0010] As the further description of the above technical scheme: a plurality of damping rods are fixedly installed on the bearing table, and the plurality of damping rods are respectively in sliding fit with the plurality of bearing columns.

[0011] As the further description of the above technical scheme: an observation window is rotatably installed on the outer side wall of the top of the culture chamber.

[0012] As the further description of the above technical scheme: a support frame is fixedly installed at the bottom of the culture chamber.

[0013] Beneficial effects: when the exosomes are taken by using the device to culture the cells, the observation window is opened, a plurality of culture dishes are placed at the top end of the bearing column, then the observation window is closed for cell culture, in the culture process, when the culture dish to be operated is taken out for operation, the culture dish to be operated is rotated to the bottom of the sample outlet, the rotation process is controlled by the motor, when the bearing column corresponding to the culture dish is rotated to the bottom of the sample outlet, the sliding block on the bearing column is clamped with the matching block, the sliding block and the matching block are driven to rise by the air cylinder, so that the bearing column is lifted, the bearing column is closely attached to the inner wall of the sample outlet, so that the inside of the culture chamber is still in a sealed environment, and external air is prevented from entering the inside of the device. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a three-dimensional structure schematic view of an exosome culture equipment according to the present application;

[0015] Figure 2 It is a three-dimensional sectional structure schematic view of the present application;

[0016] Figure 3 It is a three-dimensional sectional structure schematic view of the bearing table of the present application;

[0017] Figure 4 It is a three-dimensional sectional structure schematic view of the partition plate of the present application;

[0018] Figure 5 It is a three-dimensional structure schematic view of the sealing cover of the present application from another perspective.

[0019] LEGEND:

[0020] 1. Culture chamber; 2. Divider plate; 3. Sealing cap; 4. Sample outlet; 5. Support platform; 6. Support column; 7. Petri dish; 8. Motor; 9. Gear; 10. Gear ring; 11. Slider; 12. Cylinder; 13. Adaptor block; 14. Rubber ring; 15. Expansion port; 16. Filler block; 17. Damping rod; 18. Observation window; 19. Support frame. Detailed Implementation

[0021] To make the technical solution of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0022] Reference Figures 1-5 An exosome culture device includes a culture chamber 1, a partition plate 2 fixedly installed inside the culture chamber 1, a sealing cover 3 fixedly installed on the top of the culture chamber 1, a sample outlet hole 4 opened on the partition plate 2, a support platform 5 rotatably installed inside the culture chamber 1, a plurality of support columns 6 slidably installed on the top of the support platform 5, and a culture dish 7 is placed on the top of the plurality of support columns 6. The outer wall of the support column 6 is in contact with the inner wall of the sample outlet hole 4. During the process of cell culture to produce exosomes, when it is necessary to observe the culture dish 7 and perform other operations, the support platform 5 is rotated to rotate the selected culture dish 7 to be directly below the sample outlet hole 4. Then, the support column 6 is slid up so that the culture dish 7 extends out of the sample outlet hole 4 and the outer wall of the support column 6 is in contact with the inner wall of the sample outlet hole 4. At this time, the sealing cover 3 is opened. At this time, the culture chamber 1 is still isolated from the outside world, and the external environment will not affect the environment inside the culture chamber 1.

[0023] As a preferred embodiment, a motor 8 is fixedly installed at the bottom of the culture chamber 1, and a gear 9 is fixedly installed through the bottom of the culture chamber 1 at the output end of the motor 8. A gear ring 10 is fixedly installed at the bottom of the support platform 5, and the gear ring 10 meshes with the gear 9. Both the gear 9 and the gear ring 10 are rotatably installed at the bottom of the culture chamber 1. The motor 8 drives the gear 9 to rotate, thereby causing the gear ring 10 to rotate and thus driving the support platform 5 to rotate.

[0024] As a preferred embodiment, a slider 11 is fixedly installed on the bottom side wall of the support column 6. The slider 11 passes through the inner wall of the support platform 5 and slides with the support platform 5. A cylinder 12 is fixedly installed at the bottom of the culture chamber 1. An adapter block 13 is fixedly installed through the bottom of the culture chamber 1. The slider 11 and the adapter block 13 are engaged. The cylinder 12 and the adapter block 13 will engage with the slider 11 on the support column 6 which is rotated to the position directly below the sample outlet 4. Then, the output end of the cylinder 12 drives the adapter block 13 and the slider 11 to rise, thereby driving the support column 6 to rise.

[0025] As a preferred technical solution in this embodiment, a rubber ring 14 is fixedly installed on the top of the inner wall of the sample outlet 4, and an expansion port 15 is opened at the bottom of the sample outlet 4; the rubber ring 14 makes it fit more tightly with the inner wall of the support column 6, increasing the sealing performance, and the expansion port 15 facilitates the smooth entry of the support column 6 into the sample outlet 4.

[0026] As a preferred technical solution in this embodiment, a filling block 16 is fixedly installed at the bottom of the sealing cover 3. The filling block 16 is in contact with the top of the culture chamber 1 and the partition plate 2. When the culture chamber 1 is closed, the filling block 16 squeezes out all the external air at the top of the partition plate 2, preventing external air from remaining between the partition plate 2 and the sealing cover 3 and entering the interior of the culture chamber 1.

[0027] As a preferred technical solution in this embodiment, a plurality of damping rods 17 are fixedly installed on the support platform 5, and the plurality of damping rods 17 are respectively slidably engaged with the plurality of support columns 6; the stability of the support columns 6 during lifting and sliding is further increased by the slidable engagement between the damping rods 17 and the support columns 6.

[0028] As a preferred technical solution in this embodiment, an observation window 18 is rotatably installed on the top outer wall of the culture chamber 1; the inside of the culture chamber 1 can be simply observed through the observation window 18. When placing the culture dish 7 for the first time, the observation window 18 can be opened to quickly place the culture dish 7 into the culture chamber 1.

[0029] As a preferred technical solution in this embodiment, a support frame 19 is fixedly installed at the bottom of the culture chamber 1; the support frame 19 supports the entire culture chamber 1.

[0030] Overall work process:

[0031] When using this device to culture cells to obtain exosomes, first open the observation window 18, place the culture dishes 7 in batches on the top of the support column 6, then close the observation window 18 to continue cell culture. During the culture process, if it is necessary to remove the culture dish 7 for operation, rotate the culture dish 7 to be operated to the bottom of the sampling well. The rotation process is controlled by the motor 8. When the support column 6 corresponding to the culture dish 7 rotates to the bottom of the sampling well, the slider 11 on the support column 6 engages with the adapter block 13. The cylinder 12 drives the slider 11 and the adapter block 13 to rise, causing the support column 6 to rise. The support column 6 is tightly attached to the inner wall of the sampling well 4, so that the inside of the culture chamber 1 remains a sealed environment, preventing external air from entering the device.

[0032] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of this utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. An exosome culture device, comprising a culture chamber (1), characterized in that, A partition plate (2) is fixedly installed inside the culture chamber (1), and a sealing cover (3) is fixedly installed on the top of the culture chamber (1). A sample outlet hole (4) is opened on the partition plate (2). A support platform (5) is rotatably installed inside the culture chamber (1). Multiple support columns (6) are slidably installed on the top of the support platform (5). A petri dish (7) is set on the top of the multiple support columns (6). The outer wall of the support column (6) is in contact with the inner wall of the sample outlet hole (4).

2. The exosome culture device according to claim 1, characterized in that, A motor (8) is fixedly installed at the bottom of the culture chamber (1). A gear (9) is fixedly installed through the bottom of the culture chamber (1) at the output end of the motor (8). A gear ring (10) is fixedly installed at the bottom of the support platform (5). The gear ring (10) meshes with the gear (9). Both the gear (9) and the gear ring (10) are rotatably installed at the bottom of the culture chamber (1).

3. The exosome culture device according to claim 2, characterized in that, A slider (11) is fixedly installed on the bottom side wall of the support column (6). The slider (11) slides through the inner wall of the support platform (5) and is in sliding cooperation with the support platform (5). A cylinder (12) is fixedly installed at the bottom of the culture chamber (1). An adapter block (13) is fixedly installed through the bottom of the culture chamber (1). The slider (11) and the adapter block (13) are engaged and connected.

4. The exosome culture device according to claim 3, characterized in that, A rubber ring (14) is fixedly installed on the top of the inner wall of the sample outlet (4), and an expansion port (15) is opened at the bottom of the sample outlet (4).

5. The exosome culture device according to claim 4, characterized in that, A filling block (16) is fixedly installed at the bottom of the sealing cover (3), and the filling block (16) is in contact with the top of the culture chamber (1) and the partition plate (2).

6. The exosome culture device according to claim 5, characterized in that, Multiple damping rods (17) are fixedly installed on the support platform (5), and the multiple damping rods (17) are respectively slidably engaged with the multiple support columns (6).

7. An exosome culture device according to claim 6, characterized in that, An observation window (18) is rotatably mounted on the top outer wall of the culture chamber (1).

8. The exosome culture device according to claim 1, characterized in that, A support frame (19) is fixedly installed at the bottom of the culture chamber (1).

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