Stock solution preparation device for DNA (deoxyribonucleic acid) ploidy staining solution

Abstract

The utility model provides a device for preparing a DNA (deoxyribonucleic acid) ploidy staining solution stock solution. The device comprises a grinding and filtering assembly, a storage assembly and a conveying assembly, the grinding and filtering assembly comprises an outer shell, an inner shell is arranged in the outer shell, a gravity sensor is connected between the lower end of the inner shell and the inner bottom of the outer shell, a grinder is connected to the top of the inner shell, a filter is arranged at the lower end of the outer shell, and a guide pipe is connected between the filter and the inner shell. And a support is arranged on the outer surface of the shell. When the device is used, by arranging the material box, the alcohol barrel, the spiral conveying roller, the water conveying pipe, the inner shell, the gravity sensor, the proportional valve, the grinder and the filter, raw materials can automatically enter the inner shell, the gram weight of the raw materials is weighed, then grinding, mixing and filtering are carried out, and finally a DNA ploidy staining solution stock solution is prepared. The configuration efficiency is improved, and errors caused by manual operation are avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biotechnology, in particular to a kind of for DNA diploid staining liquid stock solution configuration device. BACKGROUND

[0002] DNA diploid staining liquid is an important reagent for cytogenetic research, mainly used for the staining of human cell DNA diploid. DNA diploid staining liquid, through specific staining mechanism, utilizes the interaction of chemical substances and DNA to stain and mark the DNA diploid state in cells for observation and analysis. It plays an important role in early diagnosis of malignant tumors, tracking follow-up and early treatment, and helps to improve the cure rate and survival rate of tumors.

[0003] In the prior art, the commonly used DNA diploid staining liquid is thionine staining liquid, which needs to be configured. A certain amount of thionine powder (such as 4g) is placed in a mortar, and 100ml of 50% ethanol is added for grinding and dissolving. This process is manually operated, and the weighing, grinding and filtering steps need to be operated separately using different instruments. The operation process is relatively complicated, and the solution is easy to spill, which causes the proportion of raw materials to be out of balance, thereby affecting the configuration of thionine staining liquid.

[0004] Therefore, we propose a DNA diploid staining liquid stock solution configuration device to solve the problem of large error and poor mixing uniformity of the stock solution in the prior art. UTILITY MODEL CONTENTS

[0005] In view of the above-mentioned shortcomings of the prior art, the purpose of the utility model is to provide a DNA diploid staining liquid stock solution configuration device to solve the problem of large error and poor mixing uniformity of the stock solution in the prior art.

[0006] To achieve the above-mentioned purposes and other related purposes, the utility model provides a DNA diploid staining liquid stock solution configuration device, comprising: a grinding and filtering assembly, a storage assembly and a conveying assembly.

[0007] The grinding and filtering assembly comprises a shell, an inner shell is provided in the inside of the shell, a gravity sensor is connected between the lower end of the inner shell and the inner bottom of the shell, a grinder is connected to the top of the inner shell, a filter is provided at the lower end of the shell, a conduit is connected between the filter and the inner shell, and a support is provided on the outer surface of the shell.

[0008] The storage assembly comprises a material box and an alcohol barrel respectively located at both ends of the shell.

[0009] The conveying assembly is used to convey the raw materials in the material box and the alcohol barrel to the inner shell.

[0010] Preferably, the grinder includes a motor, the output end of which passes through the top of the inner shell and is connected to a guide rail. The lower end of the guide rail is provided with a groove, and an electric push rod is connected to the inner side wall of the groove. The telescopic end of the electric push rod is connected to a slider that matches the groove, and the lower end of the slider is elastically connected to a grinding pestle.

[0011] Preferably, the upper end of the guide rail is rotatably connected to the inner top of the inner shell;

[0012] The slider is slidably connected to the grinding pestle, and a spring is sleeved on the outer surface of the connection.

[0013] Preferably, the filter includes a filter cylinder, the filter cylinder having a filter screen inside, and the lower end of the filter cylinder being connected to a discharge port.

[0014] Preferably, the filter cartridge includes an upper cover connected to a conduit, a rotating ring threadedly connected to the lower end of the upper cover, the rotating ring being detachably connected to the filter screen, and the lower end of the rotating ring being threadedly connected to the lower cover.

[0015] Preferably, the support includes a collar surrounding the outer surface of the housing, one end of the collar being connected to a support post, and the lower end of the support post being connected to a base.

[0016] Preferably, an electric telescopic rod is connected between the lower middle part of the outer shell and the upper middle part of the upper cover, and the electric telescopic rod is used to control the up and down movement of the filter.

[0017] Preferably, the upper end of the base is provided with a groove directly below the filter, the groove being used for placing an external collection bottle.

[0018] Preferably, the conveying assembly includes an inclined conveying pipe connected to the bottom of the material box, the other end of the inclined conveying pipe passing through the outer shell and connected to the top of the inner shell, a second motor connected to the bottom of the conveying pipe, and the output end of the second motor extending into the conveying pipe and connected to a spiral conveying roller.

[0019] Preferably, the delivery assembly further includes a pump body connected to the alcohol barrel, the output end of the pump body is connected to a water pipe, the other end of the water pipe passes through the outer shell and communicates with the inner shell, and a metering valve is installed on the water pipe.

[0020] As described above, the present invention discloses a device for preparing DNA ploidy staining stock solution, which has the following beneficial effects: The present invention, by setting up a material box, an alcohol tank, a spiral conveying roller, a water pipe, an inner shell, a gravity sensor, a metering valve, a grinder, and a filter, can automatically feed the raw materials into the inner shell and weigh them, then grind, mix, and filter them to finally prepare the DNA ploidy staining stock solution. The device realizes automated preparation of DNA ploidy staining stock solution, improves preparation efficiency, and avoids errors caused by manual operation.

[0021] Meanwhile, the device can also control the lifting and lowering of the filter, so that the outlet is inserted into the mouth of the collection bottle to prevent the DNA ploidy staining solution from spilling. It also makes it easy to disassemble the filter, replace the filter screen and clean the filter together, avoiding filter screen blockage caused by long-term use and affecting filtration efficiency.

[0022] Therefore, this utility model effectively overcomes the various shortcomings of the prior art and has high industrial application value. Attached Figure Description

[0023] Figure 1 The image shown is a perspective view of a device for preparing DNA ploidy staining stock solution according to this invention.

[0024] Figure 2 The image shown is a cross-sectional view of the outer casing of a DNA ploidy staining solution stock solution preparation device according to this invention.

[0025] Figure 3 The image shown is a cross-sectional view of a grinder used in a DNA ploidy staining solution preparation device according to this invention.

[0026] Figure 4 The image shown is an exploded view of a filter used in a DNA ploidy staining solution preparation device according to this invention.

[0027] Figure 5 The image shown is a cross-sectional view of the delivery tube of a DNA ploidy staining solution stock solution preparation device according to this invention.

[0028] Component designation explanation

[0029] 1. Grinding and filtering assembly; 10. Outer shell; 11. Inner shell; 12. Gravity sensor; 13. Grinder; 14. Filter; 15. Conduit; 16. Support;

[0030] 130. Motor 1; 131. Guide rail; 132. Slide groove; 133. Electric push rod; 134. Slider; 135. Grinding pestle; 136. Spring;

[0031] 140. Filter cylinder; 141. Filter screen; 142. Discharge port; 1400. Top cover; 1401. Rotary ring; 1402. Bottom cover; 144. Electric telescopic rod;

[0032] 160. Ring; 161. Support; 162. Base; 163. Groove;

[0033] 2. Storage components; 20. Material boxes; 21. Alcohol containers;

[0034] 3. Conveying assembly; 30. Conveying pipe; 31. Motor II; 32. Screw conveying roller; 33. Pump body; 34. Water delivery pipe; 35. Metering valve. Detailed Implementation

[0035] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification.

[0036] Please see Figures 1 to 5 It should be understood that the structures, proportions, sizes, etc., illustrated in the accompanying drawings are merely for illustrative purposes to aid those skilled in the art and are not intended to limit the scope of this invention. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in proportions, or adjustments to size, without affecting the effectiveness and purpose of this invention, should still fall within the scope of the technical content disclosed in this invention. Furthermore, the terms "upper," "lower," "left," "right," "middle," and "one" used in this specification are merely for clarity and are not intended to limit the scope of this invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of this invention.

[0037] like Figures 1-5 As shown, this utility model provides a device for preparing DNA ploidy staining stock solution, including: a grinding and filtering component 1, a storage component 2, and a delivery component 3;

[0038] The grinding and filtering assembly 1 includes a housing 10, inside which is an inner housing 11. A gravity sensor 12 is connected between the lower end of the inner housing 11 and the inner bottom of the housing 10. By directly placing the raw material into the inner housing 11, the gravity sensor 12 weighs it, eliminating the need for material transfer, reducing material loss, and lowering the risk of accidents. A grinder 13 is connected to the top of the inner housing 11, used for grinding and mixing the raw material. A filter 14 is located at the lower end of the housing 10, and a conduit 15 connects the filter 14 to the inner housing 11. The mixed solution enters the filter 14 through the conduit 15, allowing the filter 14 to filter the solution and remove impurities and undissolved anthraquinone powder. A support 16 is provided on the outer surface of the housing 10; the support 16 supports the entire grinding and filtering assembly 1.

[0039] The storage component 2 includes a material box 20 and an alcohol container 21 located at both ends of the outer casing 10. The material box 20 is used to store thion powder, and the alcohol container 21 is used to store 50% ethanol.

[0040] The conveying assembly 3 is used to convey the raw materials in the material box 20 and alcohol barrel 21 to the inner shell 11.

[0041] In one embodiment, please refer to Figures 2-3 The grinder 13 includes a motor 130. The output end of the motor 130 passes through the top of the inner shell 11 and is connected to a guide rail 131. The lower end of the guide rail 131 is provided with a groove 132. An electric push rod 133 is connected to the inner wall of the groove 132. The telescopic end of the electric push rod 133 is connected to a slider 134 that matches the groove 132. The lower end of the slider 134 is elastically connected to a grinding pestle 135. After the raw materials are added, the motor 130 is started, which drives the guide rail 131 to rotate. The guide rail 131 further drives the grinding pestle 135 to rotate through the slider 134. At the same time, the electric push rod 133 is started, which pushes the slider 134 to move left and right along the groove 132 in the guide rail 131, thereby controlling the movement range of the grinding pestle 135 and achieving the effect of effective grinding within the inner shell 11.

[0042] In one embodiment, please refer to Figures 2-3 The upper end of the guide rail 131 is rotatably connected to the inner top of the inner shell 11; the inner shell 11 supports the guide rail 131. The slider 134 is slidably connected to the grinding pestle 135, and a spring 136 is sleeved on the outer surface of the connection. The grinding pestle 135 is smaller at the top and larger at the bottom, and the outer edge of the bottom is an inclined arc surface. When the grinding pestle 135 encounters an obstacle, the arc surface at the bottom changes the direction of the resistance, compresses the spring 136, causes the spring 136 to contract, and drives the grinding pestle 135 to rise, thereby avoiding the obstacle and extending the service life of the grinder 13.

[0043] In one embodiment, please refer to Figure 4The filter 14 includes a filter cylinder 140, inside which a filter screen 141 is provided, and the lower end of the filter cylinder 140 is connected to a discharge port 142. The mixed solution is transported into the filter cylinder 140 by a conduit 15, and impurities and undissolved thion powder are filtered out by the filter screen 141. The filtered solution is discharged from the discharge port 142 for collection.

[0044] In one embodiment, please refer to Figure 4 The filter cartridge 140 includes an upper cover 1400 connected to the conduit 15. A rotating ring 1401 is threadedly connected to the lower end of the upper cover 1400. The rotating ring 1401 is detachably connected to the filter screen 141. The lower end of the rotating ring 1401 is threadedly connected to a lower cover 1402. By threading the two ends of the rotating ring 1401 to the upper cover 1400 and the lower cover 1402 respectively, it is easy to disassemble and clean, and it is also convenient to remove the filter screen 141 for cleaning and replacement.

[0045] In one embodiment, please refer to Figure 1 The support 16 includes a collar 160 surrounding the outer surface of the housing 10, one end of which is connected to a support column 161, and the lower end of the support column 161 is connected to a base 162. The support 16 can support the entire grinding and filtering assembly 1.

[0046] In one embodiment, please refer to Figure 1 and Figure 4 An electric telescopic rod 144 is connected between the lower middle part of the outer casing 10 and the upper middle part of the upper cover 1400. The electric telescopic rod 144 is used to control the up and down movement of the filter 14. This causes the outlet 142 of the filter 14 to move downward and insert into the mouth of the collection bottle, preventing solution leakage.

[0047] In one embodiment, please refer to Figure 1 The upper end of the base 162 is located directly below the filter 14 and has a groove 163. The groove 163 is used to place the external collection bottle so that the collection bottle is located directly below the filter 14. It should be noted that the bottom shape of the collection bottle matches the groove 163 and can be customized according to the actual situation.

[0048] In one embodiment, please refer to Figure 1 and Figure 5The conveying assembly 3 includes an inclined conveying pipe 30 connected to the bottom of the material box 20. The other end of the inclined conveying pipe 30 passes through the outer shell 10 and connects to the top of the inner shell 11. A second motor 31 is connected to the bottom of the conveying pipe 30. The output end of the second motor 31 extends into the conveying pipe 30 and is connected to a spiral conveying roller 32. In use, the second motor 31 is started, and the second motor 31 drives the spiral conveying roller 32 to rotate. The spiral conveying roller 32 conveys the anthurium powder flowing out of the material box 20 from the lower end to the inner shell 11. Because the conveying pipe 30 is inclined, the conveying can be stopped in time after the specified weight is reached, preventing the anthurium powder from continuing to discharge due to its own gravity, thus improving the accuracy of the discharge.

[0049] In one embodiment, please refer to Figure 1 and Figure 5 The delivery assembly 3 also includes a pump body 33 connected to the alcohol tank 21. The output end of the pump body 33 is connected to a water pipe 34, the other end of which passes through the outer shell 10 and connects to the inner shell 11. A metering valve 35 is installed on the water pipe 34. During use, based on the weight detected by the gravity sensor 12, the required proportion of 50% ethanol is calculated. The pump body 33 is then activated, drawing 50% ethanol solution from inside the alcohol tank 21 and delivering it to the inner shell 11 through the water pipe 34. During delivery, the metering valve 35 measures the amount of 50% ethanol solution, facilitating the direct and rapid delivery of the specified weight, thus improving preparation efficiency and accuracy.

[0050] The specific usage process of this utility model is as follows: Based on actual needs, a corresponding weight of raw material is determined. Motor 31 is started, driving the spiral conveyor roller 32 to rotate. The spiral conveyor roller 32 tilts and conveys the anthurium powder flowing from the material box 20 upwards into the inner shell 11. The gravity sensor 12 at the lower end of the inner shell 11 weighs the anthurium powder. After reaching the specified weight, motor 31 is turned off to stop the conveying of the anthurium powder. Pump 33 is then started, drawing 50% ethanol solution from the alcohol tank 21 and conveying it to the inner shell 11 through the water pipe 34. Simultaneously, a metering valve 35 is used for metering to ensure accurate dispensing. After both the anthurium powder and the 50% ethanol solution have been conveyed into the inner shell 11, the grinder 13 is started to grind and mix the raw materials. The uniformly mixed solution is conveyed to the filter 14 through the conduit 15, filtered by the filter 14, and finally flows into a collection bottle for storage and use.

[0051] The above embodiments are merely illustrative of the principles and effects of this utility model and are not intended to limit the scope of this utility model. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of this utility model. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this utility model should still be covered by the claims of this utility model.

Claims

1. A device for preparing stock solution of DNA ploidy staining solution, characterized in that, include: A grinding and filtering assembly (1), a storage assembly (2), and a conveying assembly (3); The grinding and filtering assembly (1) includes a housing (10), an inner shell (11) is provided inside the housing (10), a gravity sensor (12) is connected between the lower end of the inner shell (11) and the inner bottom of the housing (10), a grinder (13) is connected to the top of the inner shell (11), a filter (14) is provided at the lower end of the housing (10), a conduit (15) is connected between the filter (14) and the inner shell (11), and a support (16) is provided on the outer surface of the housing (10). The storage component (2) includes a material box (20) and an alcohol container (21) located at both ends of the outer shell (10); The conveying assembly (3) is used to convey the raw materials in the material box (20) and alcohol barrel (21) to the inner shell (11).

2. The apparatus for preparing DNA ploidy staining stock solution according to claim 1, characterized in that: The grinder (13) includes a motor (130), the output end of which passes through the top of the inner shell (11) and is connected to a guide rail (131). The lower end of the guide rail (131) is provided with a groove (132). An electric push rod (133) is connected to the inner side wall of the groove (132). The telescopic end of the electric push rod (133) is connected to a slider (134) that matches the groove (132). The lower end of the slider (134) is elastically connected to a grinding pestle (135).

3. The apparatus for preparing DNA ploidy staining stock solution according to claim 2, characterized in that: The upper end of the guide rail (131) is rotatably connected to the inner top of the inner shell (11); The slider (134) is slidably connected to the grinding pestle (135), and a spring (136) is sleeved on the outer surface of the connection.

4. The apparatus for preparing DNA ploidy staining stock solution according to claim 1, characterized in that: The filter (14) includes a filter cylinder (140), the filter cylinder (140) is provided with a filter screen (141) inside, and the lower end of the filter cylinder (140) is connected to a discharge port (142).

5. The apparatus for preparing DNA ploidy staining stock solution according to claim 4, characterized in that: The filter cartridge (140) includes an upper cover (1400) connected to a conduit (15), and a rotating ring (1401) is threadedly connected to the lower end of the upper cover (1400). The rotating ring (1401) is detachably connected to the filter screen (141), and the lower end of the rotating ring (1401) is threadedly connected to a lower cover (1402).

6. The apparatus for preparing DNA ploidy staining stock solution according to claim 1, characterized in that: The support (16) includes a collar (160) surrounding the outer surface of the housing (10), one end of which is connected to a support column (161), and the lower end of the support column (161) is connected to a base (162).

7. The apparatus for preparing DNA ploidy staining stock solution according to claim 5, characterized in that: An electric telescopic rod (144) is connected between the lower middle part of the outer shell (10) and the upper middle part of the upper cover (1400), and the electric telescopic rod (144) is used to control the up and down movement of the filter (14).

8. The apparatus for preparing DNA ploidy staining stock solution according to claim 6, characterized in that: The upper end of the base (162) is provided with a groove (163) located directly below the filter (14), and the groove (163) is used for placing the external collection bottle.

9. The apparatus for preparing stock solution of DNA ploidy staining solution according to claim 1, characterized in that: The conveying assembly (3) includes an inclined conveying pipe (30) connected to the bottom of the material box (20). The other end of the inclined conveying pipe (30) passes through the outer shell (10) and is connected to the top of the inner shell (11). The bottom of the conveying pipe (30) is connected to a second motor (31). The output end of the second motor (31) extends into the conveying pipe (30) and is connected to a spiral conveying roller (32).

10. The apparatus for preparing DNA ploidy staining stock solution according to claim 1, characterized in that: The delivery assembly (3) also includes a pump body (33) connected to the alcohol barrel (21). The output end of the pump body (33) is connected to a water pipe (34). The other end of the water pipe (34) passes through the outer shell (10) and communicates with the inner shell (11). A metering valve (35) is installed on the water pipe (34).

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