Centrifugal micro-fluidic chip

Abstract

The utility model relates to the technical field of micro-fluidic detection, and provides a centrifugal micro-fluidic chip which comprises a chip substrate, and a micro-fluidic channel is arranged on the chip substrate; a plurality of through holes arranged along the circumferential direction of the chip substrate; the top film and the bottom film are respectively arranged on the upper surface and the lower surface of the chip substrate; the top film, the bottom film and the through hole form a reaction detection groove for storing and injecting a sample; a plurality of areas with different thicknesses are arranged in the circumferential direction of the chip substrate, so that the sample quantities stored and injected in the reaction detection grooves of the areas are different. According to the utility model, the chip substrate is divided into a plurality of thickness areas, so that the sample quantities stored and injected in the reaction detection grooves of the areas are different, a plurality of sample detection areas are formed, one chip substrate can adapt to the analysis function of multiple indexes, meanwhile, the material selection is more diversified, the use amount is reduced, and the cost is reduced. And the manufacturing and detection cost can be effectively reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of micro -fluidic detection, specifically is centrifugal micro -fluidic chip. BACKGROUND

[0002] Micro -fluidic chip is the hot spot field of current micro total analysis system development. Micro -fluidic chip integrates the sample detection of biological, chemical, medical analysis process to a small chip. Micro -fluidic chip has liquid flow controllable, consumes sample and reagent little, analysis speed etc. characteristics, it can carry out the simultaneous analysis of hundreds of samples in a few minutes even shorter time, and can realize the pretreatment and analysis whole process of sample on line.

[0003] At present, micro -fluidic chip has the advantages of high integration, automation, miniaturization and parallel detection multiple samples or index, has become the important branch in the technical field of micro -fluidic chip. But the micro -fluidic chip of prior art adopts the manufacturing method of one-piece forming, the requirement to material is higher, leading to high cost. Therefore, aiming at the above problems, it is to be improved and developed. UTILITY MODEL CONTENT

[0004] The utility model provides centrifugal micro -fluidic chip, make above -mentioned problem get effective solution, specific implementation mode is as follows:

[0005] Centrifugal micro -fluidic chip, including chip substrate, be provided with micro -flow channel on the chip substrate;

[0006] Along the circumference of the chip substrate and set up multiple through -holes;

[0007] Respectively set up in the top membrane and bottom membrane of the upper surface and the lower surface of chip substrate, the top membrane and bottom membrane with the through -hole constitute the reaction detection groove for storing sample;

[0008] Along the circumferential direction of the chip substrate and set up multiple regions of different thicknesses, so that the sample amount stored in the reaction detection groove of each region is different.

[0009] As the further scheme of the utility model, the region of same thickness is symmetrically arranged along the central axis of the chip substrate.

[0010] As the further scheme of the utility model, the groove depth of the reaction detection groove of different regions is arranged between 2.0mm-5.0mm.

[0011] As the further scheme of the utility model, the chip substrate is provided with multiple liquid adding structures, and the liquid adding structures are communicated with the micro -flow channel and the reaction detection groove respectively.

[0012] As a further scheme of the utility model, the groove depths of the reaction detection grooves in different regions are set as 2.1mm, 3.1mm, 4.3mm or 2.9mm, 4.0mm, 5.0mm.

[0013] As a further scheme of the utility model, the regions include a first region, a second region and a third region, and a transition structure is arranged between two adjacent regions.

[0014] As a further scheme of the utility model, the transition structure is in a stepped shape.

[0015] As a further scheme of the utility model, the two adjacent regions are connected by a circular arc.

[0016] As a further scheme of the utility model, the number of reaction detection grooves in different regions is equal or not equal.

[0017] Due to the above technical scheme, the utility model has the beneficial technical effects that:

[0018] The chip substrate is divided into multiple thickness regions, and the reaction detection grooves are formed by upper and lower film covering, so that the sample amounts stored in the reaction detection grooves in each region are different, multiple sample detection regions are formed, one chip substrate can adapt to the analysis function of multiple indexes, the material selection is widened, the amount of substrate material is reduced, and the manufacturing and detection costs can be reduced at the same time. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a structure schematic view of the centrifugal microfluidic chip after film covering in the embodiment of the utility model;

[0020] Figure 2 It is a structure schematic view of the centrifugal microfluidic chip in the embodiment of the utility model;

[0021] Figure 3 It is a structure schematic view of the centrifugal microfluidic chip fan in the embodiment of the utility model;

[0022] Figure 4 It is a local sectional view of the utility model Figure 3 in which different regions are respectively covered with bottom films;

[0023] Figure 5 It is a local enlarged view of the centrifugal microfluidic chip in the embodiment of the utility model.

[0024] EXPLANATION OF REFERENCE NUMERALS:

[0025] 1, chip substrate, 2, top film, 3, bottom film,

[0026] 11, liquid tank, 12, airway, 13, microfluidic channel, 14, sample quantitative tank, 15, first flow channel, 16, storage tank, 17, first capillary channel, 18, mixing tank, 19, second capillary channel, 20, annular flow channel, 21, divergent radial flow channel, 22, reaction detection tank, 23, transition arc, 24, second flow channel. DETAILED DESCRIPTION

[0027] The specific embodiments of the present application will be described below with reference to the accompanying drawings and examples:

[0028] It should be noted that the structures, proportions, sizes, etc. shown in the drawings of the present application are merely used to cooperate with the content disclosed in the present application, so as to be understood and read by those skilled in the art, and are not used to limit the implementation conditions of the present application. Any modification of structure, change of proportion relationship or adjustment of size, which does not affect the functions and purposes of the present application, shall still fall within the scope of the technical content disclosed in the present application.

[0029] Meanwhile, the terms such as "upper", "lower", "left", "right", "middle" and "one" in the present application are merely used for clear understanding of the description, and are not used to limit the implementation range of the present application. The change or adjustment of relative relationship, without substantial change of technical content, is also regarded as the implementation scope of the present application.

[0030] In combination with Figures 1 to 5 As shown in the drawings, the present application provides a centrifugal microfluidic chip, which comprises a chip substrate 1, a microfluidic channel is arranged on the chip substrate 1, a plurality of through holes are arranged along the circumferential direction of the chip substrate 1, a top film 2 and a bottom film 3 are arranged on the upper surface and the lower surface of the chip substrate 1 respectively, and the top film 2 and the bottom film 3 and the through holes form a reaction detection tank 22 for storing and injecting samples.

[0031] The circumferential direction of the chip substrate 1 is divided into a plurality of regions with different thicknesses, which are fan-shaped regions, and each fan-shaped region has a plurality of reaction detection tanks 22 with the same depth and volume, so that the entire microfluidic chip has a plurality of reaction detection tank 22 groups, and the sample amounts stored and injected in the reaction detection tanks 22 of each region are different.

[0032] The present application covers the top film 2 on the top surface of the chip substrate 1, covers the bottom film 3 on the bottom surface of the chip substrate 1, so that the area between the top film 2 and the bottom film 3 and the through hole forms the reaction detection tank 22. Since the chip substrate 1 has different thickness regions, after covering the bottom film 3 on the bottom surface of the chip substrate 1, it has the function of storing and injecting different sample amounts, so that one chip substrate 1 can adapt to multiple index analysis functions.

[0033] In the prior art, the centrifugal microfluidic chip is mostly integrally injection molded. In order to ensure stable and accurate detection results, the light transmittance of the detection area, especially the ultraviolet band light (340 nm wavelength), is required to be high, so high-quality plastics, glass or quartz and other expensive materials are generally used. In fact, only the area of the reaction detection groove 22 requires high light transmittance, and other areas actually have no requirement for the light transmittance of the material, but still need to be made of expensive materials, resulting in high cost.

[0034] The utility model discloses to centrifugal microfluidic chip with parallel multiple project detection demand, adopt chip substrate 1 plus lower paste film structure, chip substrate 1 is divided into multiple thickness area along the circumference, utilize top film 2 and bottom film 3 and chip substrate 1 combine to form micro flow channel and reaction detection groove 22, form multiple detection areas on the same chip substrate 1, to satisfy the demand of multiple different project detection on the same chip substrate 1, on the one hand, the material selection of chip substrate 1 can be more diversified, and the amount can be greatly reduced, reduce the manufacturing cost of chip substrate 1, on the other hand, the detection area of the utility model chip substrate 1 only needs to set through -hole, utilize top film 2 and bottom film 3 and through -hole form reaction detection groove 22, and the detection result precision of different detection projects is reduced by the influence of the light transmittance of chip substrate 1.

[0035] Specifically, the side of the chip substrate 1 with the liquid is the front side, and the back side of the chip substrate 1 is arranged in a stepped shape to adjust the thickness of each area. The front side of the chip substrate 1 is covered with a top film 2, and the back side of the chip substrate 1 is covered with a bottom film 3. The bottom film 3 is water-tightly covered on the back side of the chip substrate 1 using a gluing process or the like, so that the top film 2 and the bottom film 3 completely seal the two open ends of the reaction detection groove 22.

[0036] Specifically, the micro flow channel includes a branch flow channel and a divergent radial flow channel 21 that are sequentially connected from the proximal end to the distal end of the chip substrate 1. The branch flow channel is in communication with the liquid addition groove 11, and the liquid addition groove 11 and the branch flow channel are in communication with a second flow liquid channel 24 and a vent groove 12. The divergent radial flow channel 21 is in communication with the reaction detection groove 22. The sample is output from the divergent radial flow channel 21 to the reaction detection groove 22, reacts with the pre-added reagent in the reaction detection groove 22, and realizes multi-index quantitative detection of the sample.

[0037] Specifically, the flow distribution channel further comprises, sequentially from the proximal end to the distal end, a sample quantification groove 14, a storage groove 16, a mixing groove 18, a second capillary channel 19, and an annular flow channel 20. The micro flow channel 13 is in communication between the sample quantification groove 14 and the vent groove 12. The first flow liquid channel 15 is in communication between the sample quantification groove 14 and the storage groove 16. The mixing groove 18 is in communication with the first capillary channel 17. The annular flow channel 20 is in communication with the divergent radial flow channel 21. After the sample injection and liquid addition groove 11, the sample is output to the storage groove 16 through the second flow liquid channel 24, the vent groove 12, the micro flow channel 13, and the first flow liquid channel 15, respectively. The sample in the storage groove 16 is output to the mixing groove 18, and then output to the second capillary channel 19. The sample in the second capillary channel 19 is output to the annular flow channel 20, and then output to the reaction detection groove 22, so as to realize the multi-index quantitative detection of the sample.

[0038] Exemplarily, the annular flow channel 20 is arranged along the chip substrate 1 for one circle, so that the sample output to the annular flow channel 20 is distributed to the reaction detection groove 22.

[0039] Specifically, as shown in Figure 3 , the regions with the same thickness are symmetrically arranged about the central axis of the chip substrate 1. The central axis is the rotation axis of the chip substrate 1 in the centrifugal operation state, and the regions with the same thickness are symmetrically arranged along the rotation axis of the chip substrate 1. In this embodiment, the chip substrate 1 has three pairs of equal fan-shaped regions, and the top angle α of each pair of fan-shaped regions is 60°. The fan-shaped regions symmetrically arranged about the rotation axis of the chip substrate 1 have the same thickness, and can at least meet three different detection items. Figure 4 is Figure 3 the cross-sectional view of the reaction detection groove 22 after the chip is cut along A-A, B-B, and C-C. In this embodiment, the chip substrate 1 is provided with at least three and at least six groups of micro flow channels, and the liquid addition structure is in communication with the micro flow channel and the reaction detection groove 22, so as to form a plurality of sample detection regions on the chip substrate 1.

[0040] The optical detection obtained absorbance value is proportional to the depth of the reaction detection groove and the concentration of the detection item in the sample. The absorbance value is too large or too small, which exceeds the linear detection range of the optical detection equipment. When the detection sample is determined, the depth of the reaction detection groove can be used to adjust the size of the absorbance value, so that the absorbance values of different detection items are within the linear detection range of the optical detection equipment. Preferably, the groove depth of the reaction detection groove 22 in different regions is set to be between 2.0mm-5.0mm. When the chip substrate 1 detects the low-concentration item in the sample, the chip substrate 1 can be set to have different region thicknesses of 4.0mm, 4.3mm and 5.0mm. The deeper reaction detection groove 22 can ensure that the absorbance value obtained by the reaction detection is relatively large and within the linear detection range of the optical detection equipment. When the chip substrate 1 detects the high-concentration item in the sample, the different region thicknesses of 2.1mm and 3.1mm can be selected. The shallower reaction detection groove 22 can ensure that the absorbance value obtained by the reaction detection is relatively small and within the linear detection range of the optical detection equipment.

[0041] In a preferred embodiment of the present application, the entire chip is divided into a first region, a second region and a third region according to different thicknesses, and a transition structure is arranged between adjacent two regions. The transition structure is in a stepped shape. For example, the transition structure is arranged between the first region and the second region, between the second region and the third region, and between the third region and the first region, and the transition structure is in a stepped shape, so that the equal division regions are connected through the stepped transition structure. This design can reduce the process difficulty.

[0042] Preferably, as shown in Figure 5 The adjacent two regions are connected through a circular arc transition. The first region and the second region, the second region and the third region, and the third region and the first region are connected through a circular arc transition, which is a transition circular arc 23. The transition circular arc 23 is used to connect the first region, the second region and the third region, so that the bottom film 3 can be more closely attached to the back of the chip substrate 1.

[0043] Specifically, the number of reaction detection grooves 22 in each region is equal or unequal. The number of reaction detection grooves 22 in the first region, the second region and the third region is equal; the number of reaction detection grooves 22 in the first region, the second region and the third region is unequal. Different samples detect different items, and the number of reaction detection grooves 22 in each region can be equal or unequal.

[0044] The utility model discloses a working principle is, with blood sample detection as an example, first respectively cover top membrane 2 and bottom membrane 3 in the front and back of chip substrate 1, when the chip substrate 1 rotates, the sample that injects in liquid tank 11 is exported to sample quantitative tank 14 in second liquid passage 24 and microfluidic channel 13 to blood cell after centrifugal separation is stored in storage tank 16, and the plasma enters into mixed tank 18 in first capillary channel 17. The sample in mixed tank 18 is exported to annular flow channel 20 in second capillary channel 19, finally, the chip substrate 1 continues to rotate according to the preset speed, and the plasma in annular flow channel 20 is shunted into the respective corresponding reaction detection tank 22 of prepackaged reagent in divergent radial flow channel 21, and the corresponding detection result is obtained after reaction.

[0045] Many other changes and modifications can be made without departing from the scope and spirit of the utility model. It should be understood that the utility model is not limited to the specific embodiments, and the scope of the utility model is defined by the appended claims.

Claims

1. A centrifugal microfluidic chip, characterized by, The chip substrate (1) is provided with micro flow channels; A plurality of through holes are arranged along the circumference of the chip substrate (1); A top film (2) and a bottom film (3) are respectively arranged on the upper surface and the lower surface of the chip substrate (1), and the top film (2) and the bottom film (3) and the through holes form a reaction detection groove (22) for storing samples; A plurality of regions with different thicknesses are arranged along the circumferential direction of the chip substrate (1), so that the sample amount stored in the reaction detection groove (22) of each region is different.

2. The centrifugal microfluidic chip of claim 1, wherein, The regions with the same thickness are symmetrically arranged along the central axis of the chip substrate (1).

3. The centrifugal microfluidic chip of claim 2, wherein, The chip substrate (1) is provided with a plurality of liquid adding structures which are respectively communicated with the micro flow channels and the reaction detection groove (22).

4. The centrifugal microfluidic chip of claim 2, wherein, The groove depth of the reaction detection groove (22) of the different regions is arranged to be between 2.0mm-5.0mm.

5. The centrifugal microfluidic chip of claim 4, wherein, The groove depth of the reaction detection groove (22) of the different regions is arranged to be 2.1mm, 3.1mm, 4.3mm or 2.9mm, 4.0mm, 5.0mm.

6. The centrifugal microfluidic chip of claim 1 wherein, The regions include a first region, a second region, and a third region, and a transition structure is arranged between two adjacent regions.

7. The centrifugal microfluidic chip of claim 6, wherein, The transition structure is in a stepped shape.

8. The centrifugal microfluidic chip of claim 6, wherein, The transition between two adjacent regions is in a circular arc shape.

9. The centrifugal microfluidic chip of claim 1 wherein, The number of reaction detection grooves (22) on the different regions is equal or unequal.

Images