Micro-fluidic chip

Abstract

The utility model provides a micro-fluidic chip, relates to the technical field of micro-fluidic detection, and solves the technical problem that a centrifugal micro-fluidic chip in the prior art cannot directly observe whether the sample size in a sample adding groove is proper or not. The device comprises a chip substrate and a sealing membrane packaged on the upper surface of the substrate, a flat long groove is formed along the upper surface of the substrate, the flat long groove and the sealing membrane form a sample adding groove, a sample adding opening is formed in the sealing membrane, and the sample adding opening is communicated with the sample adding groove and used for injecting a sample into the sample adding groove; and a supporting device is arranged and is used for supporting the sealing membrane on the sample adding groove. By designing the structure of the sample adding groove, an operator can visually observe whether the sample adding process and the sample adding amount are appropriate or not.

Description

TECHNICAL FIELD

[0001] The utility model relates to microfluidic detection technical field especially is related to a microfluidic chip. BACKGROUND

[0002] Microfluidics chip technology occupies an extremely important position in the biomedical field, especially in nucleic acid testing (NAT), due to its unique advantages of miniaturization and high integration. The essence of this technology lies in the integration of sample pretreatment, mixing, chemical reaction, separation, and detection on one or more tiny chips, thus creating a miniature laboratory. Microfluidic chips greatly reduce the sample and reagent requirements, simplify the operation process, and significantly shorten the detection period, while effectively avoiding various errors that may occur during manual operation in traditional laboratories. Therefore, microfluidic technology has become the preferred technology in many fields such as chemical analysis, DNA sequencing, protein analysis, single-cell and single-molecule analysis, food safety monitoring, environmental monitoring, and drug screening. With continuous research and development, the application range of microfluidic chips is expanding, and their potential is enormous, making them a key technology that may have a significant impact on human lifestyle in the future.

[0003] The current microfluidic chip has a planar structure as shown in Figure 1 The upper layer and the lower layer are water-tight connected, the chip upper layer is equipped with a group of sample adding through holes for sample adding, and the lower layer corresponding to the sample adding through hole is provided with a sample adding groove for accommodating the sample injection through hole. During centrifugation, the liquid sample enters the detection hole through various functional grooves and different shaped microflow channels provided in the lower layer under the action of centrifugal force, and receives optical detection. In this process, the sample needs to pass through a long channel and multiple separations, and only by injecting a sufficient amount of sample can the sample amount entering the detection hole be sufficient for effective detection. Therefore, the existing sample adding groove is as large as possible in depth, and its capacity is generally greater than the sample amount required for one detection. In order to prevent sample overflow, the volume of the sample adding groove designed in the existing chip is much larger than the required sample volume. After sample adding, the sample falls at the bottom of the sample adding groove, and it is not possible to visually observe whether the sample adding amount is appropriate. Too much or too little sample amount will affect the function of the microfluidic chip, and thus affect the detection result. Therefore, there is an urgent need to develop a new type of microfluidic chip to solve the above technical problems. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a microfluidic chip to solve the related technical problems in the prior art. The preferred technical solutions in the many technical solutions provided by the utility model can produce many technical effects, which are described in detail below.

[0005] To achieve the above object, the utility model provides the following technical scheme:

[0006] The utility model provides a kind of microfluidic chip, comprising: chip substrate, sample addition groove is opened on it, for temporary storage sample;Sealing diaphragm, it is covered on chip substrate, sample addition port is opened on it, the sample addition port is communicated with the sample addition groove, for injecting sample to the sample addition groove;Multiple detection holes, it is evenly arranged along the circumferential direction of chip substrate, for accommodating sample to be detected;Microflow channel, for connecting sample addition groove and detection hole;The sample addition groove is extended and opened along the upper surface of the chip substrate, and it is overall in long and flat groove shape;The substrate and the sealing diaphragm constitute the sample addition groove.

[0007] According to one embodiment of the utility model, support assembly is provided, the support assembly includes the support ring for supporting sealing diaphragm installed on chip substrate, the outer circumferential side wall of the support ring is fixedly connected on the side wall of sample addition groove, through cavity is arranged in the center of the support ring, the sample addition port is communicated with the upper end of through cavity, the lower end of through cavity is communicated with sample addition groove.

[0008] According to another embodiment of the utility model, support assembly is provided, the support assembly includes the support ring for supporting sealing diaphragm installed on chip substrate, one end of the support ring is abutted on the groove bottom of sample addition groove and is fixedly connected on chip substrate, the other end of the support ring is abutted on sealing diaphragm, through cavity is arranged in the center of the support ring, the bottom end of the support ring is opened and communicated with through cavity along the direction perpendicular to through cavity, the sample addition port is communicated with the upper end of through cavity, the sample addition groove is communicated with through cavity by gap.

[0009] According to another embodiment of the utility model, support assembly is provided, the support assembly includes the support ring for supporting sealing diaphragm installed on chip substrate, one end of the support ring is abutted on the groove bottom of sample addition groove and is fixedly connected on chip substrate, the other end of the support ring is abutted on sealing diaphragm, the support ring is arranged as half circular ring, through cavity is arranged in the center of the support ring, the circumferential side of the support ring is opened and communicated with through cavity along through cavity penetration direction, the sample addition port is communicated with the upper end of through cavity, the sample addition groove is communicated with through cavity by gap.

[0010] Further, the gap is radially expanded outward, i.e., the inner port distance of the gap is less than the outer port distance of the gap.

[0011] Preferably, the top surface of the support ring and the sealing diaphragm are fixedly connected with each other.

[0012] According to another embodiment of the utility model, the sample addition groove is arranged in an arc shape starting from the sample addition port, and the sample addition groove is arranged along the circumferential direction of the chip substrate.

[0013] Further, the support assembly further comprises a plurality of support pieces arranged uniformly along the extension direction of the sample adding groove, one end of the support piece being fixedly connected to the chip substrate, and the other end of the support piece being fixedly connected to the sealing membrane.

[0014] Further, the support assembly further comprises a support piece arranged along the extension direction of the sample adding groove, the support piece being arranged in an arc shape and located at the middle of the sample adding groove.

[0015] According to another embodiment of the present application, a quantitative line is arranged on the sealing membrane, for indicating the sample quantity.

[0016] The following are the main technical effects of the present application:

[0017] The present application arranges the sample adding port and the flat-groove arc-shaped sample adding groove on the sealing membrane, so that the operator can intuitively observe the sample adding process and whether the quantity is appropriate, thereby ensuring that an appropriate amount of sample is injected each time, and improving the success rate of detection and the accuracy of the result.

[0018] The present application introduces a support assembly to support the sealing membrane, especially below the sample adding port, effectively preventing the deformation or collapse of the sealing membrane, and avoiding the problem of insufficient sample adding due to the deformation of the membrane.

[0019] In summary, the present application innovatively improves the prior art, solves the technical problems that are prone to occur in the sample adding process of the existing microfluidic chip, provides a new solution for the development of microfluidic chip technology, and is expected to greatly improve the efficiency and result accuracy of related project detection in the field of biological detection. BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or prior art description. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0021] Figure 1 is a plan view of the sample adding structure of the centrifugal microfluidic chip in the prior art;

[0022] Figure 2 is a schematic diagram of the overall structure provided by embodiment 1 of the present application;

[0023] Figure 3 is a schematic diagram of the internal structure provided by embodiment 1 of the present application;

[0024] Figure 4 is a part structure schematic view provided by the embodiment 1 of the utility model;

[0025] Figure 5 is a part structure schematic view provided by the embodiment 2 of the utility model;

[0026] Figure 6 is a part structure schematic view provided by the embodiment 3 of the utility model;

[0027] Figure 7 is a part structure schematic view provided by the embodiment 4 of the utility model;

[0028] Figure 8 is a part structure schematic view provided by the embodiment 5 of the utility model;

[0029] Figure 9 is a part structure schematic view provided by the embodiment 6 of the utility model;

[0030] Figure 10 is a part structure schematic view provided by the embodiment 6 of the utility model;

[0031] The figure mark explanation: 100, chip substrate;110, sealing diaphragm;120, detection hole;130, sample adding groove;140, micro flow channel;150, sample adding port;200, support assembly;210, support ring;220, through cavity;230, notch;240, support sheet;250, quantitative line. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical scheme and advantage of the utility model more clear, the technical scheme of the utility model will be described in detail below. Obviously, the described embodiment is only a part of the embodiment of the utility model, not all the embodiment. Based on the embodiment in the utility model, all other embodiments obtained by the ordinary skill in the art without making creative labor belong to the range of the utility model.

[0033] In the description of the utility model, it is necessary to explain that, unless otherwise stated, the meaning of "multiple" is two or more than two;The orientation or position relation indicated by the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail" and the like is based on the orientation or position relation shown in the drawing, only for the convenience of describing the utility model and simplifying the description, and not indicating or implying that the indicated device or element must have a particular orientation, a particular orientation and operation, therefore, it cannot be understood as the limitation of the utility model. In addition, the terms "first", "second", "third" and the like are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0034] In the description of the utility model, still need to explain, unless another explicit provision and limitation, term " install ", " link ", " connection " should do broad sense understanding, for example, can be fixed connection, also can be detachable connection, or integrally connected;Can be mechanical connection, also can be electrical connection;Can be direct connection, also can through the intermediate medium indirectly connect.For ordinary skilled person in the art, the above-mentioned term can be understood according to the specific circumstances the specific meaning in the utility model.

[0035] The following will be described in detail in combination with the accompanying drawings Figures 2-10 Further detailed description of the present application, the embodiment of the present application discloses a kind of microfluidic chip.It includes chip substrate and the sealing membrane piece packaged in substrate upper layer, substrate is opened with various functional grooves and different shape microflow channel, for placing and carrying the required test sample, in order to facilitate operation and improve space utilization, this chip substrate is preferably designed as disc shape.

[0036] Specifically, as Figures 2-3 Indicated, chip substrate 100 is opened with sample addition groove 130 close to the position of substrate center, and multiple detection holes 120 are opened on chip substrate 100.Multiple detection holes 120 are close to the outer edge of chip substrate 100 and are uniformly arranged along the circumferential direction of chip substrate 100, which ensures that the sample (such as biological liquid sample of blood or urine) can be uniformly distributed between each detection point, improving the consistency and reliability of detection.

[0037] Chip substrate 100 is further designed with microflow channel 140 connecting sample addition groove 130 and multiple detection holes 120.The design of microflow channel 140 not only ensures that the liquid sample can smoothly flow from sample addition groove 130 to each detection hole 120, but also can flexibly adjust the flow characteristics and residence time of the sample according to different detection requirements by adjusting the design (such as width, depth and path) of microflow channel 140, thereby optimizing the detection effect.In addition, the notch of sample addition groove 130, the hole end of detection hole 120 and the opening of microflow channel 140 are all located on the top surface of chip substrate 100, which facilitates the operator to add sample and observe.

[0038] Sealing membrane piece 110 is made of transparent material and is glued and fixed on the upper surface of substrate 100, which constitutes a sample temporary storage area with sample addition groove 130 on substrate 100, maintaining the sealing and stability of the microfluidic chip.Sealing membrane piece 110 is opened with sample addition port 150, which is communicated with sample addition groove 130, allowing the operator to inject the sample to be detected into sample addition groove 130 through the sample addition port 150.This design not only ensures the leak-free addition of sample, but also simplifies the operation steps and improves the work efficiency.

[0039] The sample adding slot 130 is long and flat as a whole, i.e. the length and width extend along the upper surface of the chip substrate 100, the height is along the thickness direction of the chip, and the width is greater than the height, and the cross section is a long rectangle or a similar long rectangle. In this way, the long and flat sample adding slot 130 is formed between the chip substrate 100 and the sealing film 110. After the sample is added to the sample adding slot 130, the sample is in contact with the surface of the substrate 100 and the sealing film 110. Under the action of capillary force, the sample can flow along the slot wall of the sample adding slot 130 and the surface of the sealing film 110 to the end of the sample adding slot 130 away from the sample adding port 150, until the entire sample adding slot 130 is filled. In this way, the operator can directly observe the amount of liquid sample added. By accurately designing the capacity of the sample adding slot 130, the amount of sample added can be ensured to be close to the required amount of sample for detection.

[0040] The material of the sealing film 110 can be plastic, glass or quartz, etc. In an embodiment, the sealing film 110 can be a plastic film which has a certain elasticity and can be deformed to a certain extent without being torn under the action of appropriate external force. In this embodiment, the size of the sample adding hole 150 is designed to be matched with the sample adding gun head. When the sample adding gun head is inserted into the sample adding hole 150, the sample adding hole 150 and the outer wall of the sample adding gun head form a close fit, thereby sealing the sample adding hole 150, sealing one end of the sample adding slot 130. When adding the sample, under the action of liquid pressure, the sample flows to the other end of the sample adding slot 130, so that the sample quickly fills the entire sample adding slot 130.

[0041] In addition, in order to make the sample fill the sample adding slot 130 more smoothly and prevent the sample from accumulating or even overflowing at the sample adding port 150, in one of the embodiments, the surface of the substrate 100 and the surface of the sealing film 110 on the inner wall of the sample adding slot 130 are treated to be hydrophilic, for example, a hydrophilic agent is coated on the surface of the substrate 100 and / or the surface of the sealing film 110.

[0042] In addition, in order to accurately control the amount of sample added, the present application also provides a sample adding quantitative line 250. The quantitative line 250 can be arranged on the bottom of the sample adding slot 130, or on the sealing film 110 and arranged along the extension direction of the fan shape. When the sample is added to the quantitative line 250, it indicates that the amount of sample added after passing through the microfluidic channel and the functional groove is sufficient to fill all the detection holes 120 without overflowing, which achieves the ideal liquid amount required for sample detection. This design not only simplifies the operation process and avoids the situation of adding too much or too little sample, but also ensures the consistency and accuracy of each detection, greatly improves the reliability and repeatability of the detection results.

[0043] Figure 10 is a schematic diagram for comparing the technical effects of the present application and the prior art sample adding slot technology. As Figure 10A, the existing sample adding slot is deep, and the operator cannot observe the sample amount and cannot directly observe whether the sample amount is sufficient; Figure 10 B, in the improved structure of the present application, after the sample adding gun adds the sample from the sample adding port, the operator can directly observe the sample adding amount change from one side of the sealing film, for example, whether the sample adding amount reaches the quantitative line position, and accurately master the sample adding amount.

[0044] Referring to Figure 3 and Figure 4 , in one embodiment of the present application, the sample adding slot 130 is designed to extend in the circumferential direction of the chip substrate 100 in an arc shape, and its top view presents a fan-shaped distribution. That is, the sample adding slot 130 has a first arc-shaped side wall close to the center of the substrate and a second arc-shaped side wall away from the center of the substrate, wherein the second arc-shaped side wall gradually moves away from the center of the chip along one end (the sample adding port end) of the sample adding slot 130 to the other end, so that the sample adding slot 130 gradually moves away from the center of the substrate from the sample adding port. This unique design not only increases the physical capacity of the sample adding slot 130, so that the sample adding slot 130 can more efficiently accommodate all the samples required in the detection process, but also enables all the samples in the sample adding slot 130 to enter the functional groove and the microfluidic channel during centrifugation, reducing sample waste and improving detection efficiency. In addition, the cross-sectional area of the sample adding slot 130 gradually increases from one end to the other end, which is beneficial to the rapid flow of the sample into the sample adding slot, thereby avoiding the aggregation of the sample near the sample adding port during sample adding. In addition, such a design also makes it easier for the operator to observe the sample adding condition, enhancing the intuitiveness and convenience of the operation.

[0045] It is worth noting that when the upper layer of the microfluidic chip is packaged with a film material, the film material is easily deformed under the extrusion of the sample adding gun during sample adding, and the actual capacity of the deformed sample adding slot is less than the designed capacity, which is easy to cause insufficient sample amount. In view of the above shortcomings, the following embodiments will detail various specific embodiments of the present application.

[0046] Embodiment 1

[0047] Referring to Figure 3 and Figure 4 , in one embodiment of the present application, the chip substrate 100 is also provided with a support assembly 200. The support assembly 200 includes a support ring 210 fixedly installed on the chip substrate 100, the bottom end of the support ring 210 is fixedly connected to the groove bottom of the sample adding slot 130, and the top end is fixedly connected to the sealing film 110, and the support ring 210 and the sealing film 110 can be connected to each other by means of gluing, ensuring the stability of the structure. And the support ring 210 is arranged at one end of the sample adding slot 130 away from the communication point of the microfluidic channel 140, avoiding interference with the microfluidic channel 140 during sample adding, and ensuring the smooth flow of the sample flow path.

[0048] The center of the support ring 210 is provided with a through cavity 220, and the top end of the through cavity 220 corresponds to and communicates with the sample adding port 150 on the sealing diaphragm 110. The sample adding gun or sample containing tube can smoothly add the sample into the through cavity 220 through the sample adding port 150 and guide the sample into the inside of the sample adding groove 130. The side wall of the support ring 210 is provided with a notch 230 arranged in the height direction and communicating with the through cavity 220, that is, the support ring 210 is arranged in a semi-circular ring. Such a design not only provides a direct inflow path for the sample, but also plays a guiding role in the sample adding process to prevent the sample from overflowing or splashing out, thereby ensuring the cleanliness and safety of the sample adding process.

[0049] The notch 230 is radially expanded outward, that is, the inner port of the notch 230 is smaller than the outer port of the notch 230, that is, the four vertices of the notch 230 are connected to each other to form a stepped shape. In this way, the sample in the through cavity 220 can flow outward, that is, after the sample enters the through cavity 220, it can directly flow out of the notch 230 into the sample adding groove 130, avoiding the accumulation of the sample in the through cavity 220.

[0050] When the sample adding gun or sample containing tube injects the sample into the sample adding groove 130 through the sample adding port 150, the top end of the support ring 210 can rigidly support the sample adding gun or sample containing tube, effectively supporting these devices and preventing the sealing diaphragm 110 from deforming or collapsing due to uneven pressure. If the sealing diaphragm 110 deforms or collapses, unnecessary fitting between the sample adding groove 130 and the sealing diaphragm 110 can occur, hindering the normal inflow of the sample and even causing the sample adding to fail. Therefore, the design of the support ring 210 not only ensures the smooth progress of the sample adding process, but also significantly improves the safety and reliability of the operation.

[0051] In addition, a groove is arranged below the support ring 210 and in the sample adding groove 130. The design of the groove further enhances the space utilization of the sample adding groove 130 and provides additional space for the sample. When the sample is injected into the sample adding groove 130 through the sample adding port 150 and the through cavity 220, the accommodating space of the groove can prevent the added sample from flowing back and overflowing due to excessive speed and content.

[0052] The support assembly 200 can effectively support the sealing diaphragm 110, prevent the sealing diaphragm 100 from irreversibly deforming under external force, avoid the deformation or collapse of the sealing diaphragm 110 caused by external force during the liquid injection process, avoid the failure of the sample adding groove 130, and ensure the structural integrity and functional stability of the microfluidic chip during use.

[0053] Example 2

[0054] The main difference between this embodiment and embodiment 1 is the improved design of the support assembly 200, which is embodied in the use of a support sheet 240.

[0055] Referring to Figure 5 As shown in the drawings, the support assembly 200 in this embodiment includes a support sheet 240 disposed along the arc extension direction of the sample loading groove 130, one end of which is close to the support ring 210 and the other end of which extends to the communication point between the sample loading groove 130 and the microfluidic channel 140. This enables the support sheet 240 to cover the key area of the sample loading groove 130 and provide more extensive support. The bottom end of the support sheet 240 is fixedly connected to the groove bottom of the sample loading groove 130, and the top end is fixedly connected to the sealing diaphragm 110. The support sheet 240 is disposed on the center dividing line of the sample loading groove 130, ensuring the symmetry and stability of the structure.

[0056] The added support sheet 240 can provide additional support to the entire sealing diaphragm 110, effectively preventing the internal air pressure of the sealing diaphragm 110 from changing during the sample loading process, causing deformation or collapse at the non-sample loading port 150 position. The combined design of the support sheet 240 and the support ring 210 significantly enhances the structural strength of the entire support assembly 200. This enhancement not only improves the pressure resistance of the sample loading groove 130, but also to some extent guides the flow direction of the sample.

[0057] Embodiment 3

[0058] The main difference between this embodiment and embodiment 2 is the improved design of the support assembly 200, which is embodied in the reduction of the use of the support ring 210.

[0059] Referring to Figure 6 As shown in the drawings, the support assembly 200 in this embodiment only includes a support sheet 240 disposed along the arc extension direction of the sample loading groove 130, one end of which is close to the support ring 210 and the other end of which extends to the communication point between the sample loading groove 130 and the microfluidic channel 140. This enables the support sheet 240 to cover the key area of the sample loading groove 130 and provide more extensive support. The bottom end of the support sheet 240 is fixedly connected to the groove bottom of the sample loading groove 130, and the top end is fixedly connected to the sealing diaphragm 110. The support sheet 240 is disposed on the center dividing line of the sample loading groove 130, ensuring the symmetry and stability of the structure.

[0060] By canceling the support ring 210, the structure of the support assembly 200 becomes more concise. This not only reduces the manufacturing cost, but also reduces the process difficulty and improves the production efficiency.

[0061] Although the support ring 210 is cancelled, the design of the support sheet 240 can still provide sufficient support for the sealing diaphragm 110. The two ends of the support sheet 240 are fixed on the groove bottom of the sample loading groove 130 and the sealing diaphragm 110 respectively, which ensures the stability of the sealing diaphragm 110 under pressure, prevents local deformation or collapse, and thus guarantees the stability and continuity of the sample loading process.

[0062] The extension of the support sheet 240 can provide a guiding effect for liquid flow, help the sample reach the communication point of the micro-flow channel 140 faster, reduce the residence time of the sample in the sample loading groove 130, and improve the response speed and efficiency of the whole system.

[0063] Embodiment 4

[0064] The main difference between this embodiment and embodiment 3 is the improved design of the support assembly 200, which is specifically reflected in the structural shape of the support sheet 240.

[0065] Referring to Figure 7 As shown in the figure, the support assembly 200 includes a plurality of support sheets 240 for supporting the sealing diaphragm 110, and the plurality of support sheets 240 are uniformly arranged along the extension direction of the sample loading groove 130. This ensures uniform support of the sealing diaphragm 110 along the entire length of the sample loading groove 130, avoiding deformation or damage caused by uneven local stress.

[0066] One end of each support sheet 240 is fixedly connected to the chip substrate 100, i.e. the groove bottom of the sample loading groove 130, and the other end of the support sheet 240 is fixedly connected to the sealing diaphragm 110. This fixing method ensures the stability of the support sheet 240 and the flatness of the sealing diaphragm 110.

[0067] The support sheet 240 is arranged in an arch shape, i.e. the middle lower end of the support sheet 240 is left with space, providing a smooth flow path for the sample. This not only reduces liquid surface fluctuation, but also ensures uniform distribution of the sample in the sample loading groove 130, improving the accuracy and consistency of the detection results.

[0068] Embodiment 5

[0069] The main difference between this embodiment and embodiment 1 is the improved design of the support assembly 200, which is specifically reflected in the structural shape of the support ring 210.

[0070] Referring to Figure 8As shown, the support assembly 200 in this embodiment includes a support ring 210 fixedly mounted on the chip substrate 100. The outer circumferential sidewall of the support ring 210 is fixedly connected to the sidewall of the sample loading groove 130, ensuring a stable connection between the support ring 210 and the sample loading groove 130. The bottom end of the support ring 210 is kept a certain distance from the groove bottom of the sample loading groove 130, while the top end is fixedly connected to the sealing diaphragm 110. This design not only ensures the stability of the structure, but also provides space for the flow of the sample.

[0071] By fixedly connecting the outer circumferential sidewall of the support ring 210 to the sidewall of the sample loading groove 130, this fixed manner ensures a stable connection between the support ring 210 and the sample loading groove 130. Even under external pressure or vibration, the support ring 210 can remain stable, avoiding the risk of structural loosening or deformation.

[0072] The center of the support ring 210 is provided with a through cavity 220, and the top end of the through cavity 220 corresponds to and communicates with the sample loading port 150 on the sealing diaphragm 110. The bottom end of the through cavity 220 also communicates with the groove bottom of the sample loading groove 130, so that the sample can smoothly enter the through cavity 220 through the sample loading port 150, and then flow into the groove bottom of the sample loading groove 130 from the bottom end of the through cavity 220, and flow along the extension direction of the sample loading groove 130.

[0073] The center through cavity 220 of the support ring 210 provides a clear flow path for the sample. The sample enters the through cavity 220 through the sample loading port 150, and then flows into the groove bottom of the sample loading groove 130 from the bottom end of the through cavity 220, and flows smoothly along the extension direction of the sample loading groove 130. This design reduces liquid level fluctuations, ensures uniform distribution of the sample, and improves the accuracy of the detection results.

[0074] Embodiment 6

[0075] The main difference between this embodiment and Embodiment 1 is the improved design of the support assembly 200, which is specifically manifested in the structural shape of the support ring 210.

[0076] Reference Figure 9 As shown, the support assembly 200 in this embodiment includes a support ring 210 fixedly mounted on the chip substrate 100. The bottom end of the support ring 210 is fixedly connected to the groove bottom of the sample loading groove 130, while the top end is fixedly connected to the sealing diaphragm 110, ensuring the stability of the structure. And the support ring 210 is arranged at one end of the sample loading groove 130 away from the communication point of the micro-flow channel 140, avoiding interference with the micro-flow channel 140 during the sample loading process, and ensuring the smooth flow path of the sample.

[0077] The center of the support ring 210 is provided with a through cavity 220, and the top end of the through cavity 220 corresponds to the sample adding port 150 on the sealing diaphragm 110 and communicates with each other. This design allows the sample adding gun or the liquid sample containing tube to smoothly add the sample into the through cavity 220 through the sample adding port 150 and guide to the inside of the sample adding groove 130.

[0078] The side wall of the support ring 210 is provided with a notch 230 communicating with the through cavity 220, the notch 230 is arranged in a direction perpendicular to the through cavity 220, and the notch 230 is also arranged at the bottom end of the support ring 210 and communicates with the sample adding groove 130. When the sample adding gun or the liquid sample containing tube injects the sample into the sample adding groove 130 through the sample adding port 150, the sample flows out from the notch 230 into the sample adding groove 130 after passing through the through cavity 220.

[0079] The center through cavity 220 of the support ring 210 is designed to allow the sample to smoothly flow from the sample adding port 150 into the through cavity 220 and then flow out from the notch 230 in the side wall into the sample adding groove 130. The vertical arrangement of the through cavity 220 and the notch 230 can reduce the turbulence and vortex generated during the flow of the liquid. When the sample flows from the through cavity 220 into the notch 230, the vertical flow reduces the horizontal disturbance, making the liquid flow more stable, reducing the formation of bubbles, and helping to accurately judge the sample addition result and avoiding the interference of bubbles.

[0080] In summary, the support assembly 200 designed with care not only solves various problems that may occur during the sample adding process, such as deformation of the sealing diaphragm 110, sample overflow, etc., but also improves the precision and stability of sample adding, laying a solid foundation for efficient and reliable biological sample detection.

[0081] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto, and any skilled person in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A microfluidic chip, characterized by, The chip substrate (100) has a sample adding groove (130) for temporarily storing a sample. A sealing film (110) covers the chip substrate (100) and has a sample adding opening (150) communicating with the sample adding groove (130) for injecting the sample into the sample adding groove (130). A plurality of detection holes (120) are uniformly arranged along the circumferential direction of the chip substrate (100) and are used for containing samples to be detected. A micro flow channel (140) is used for connecting the sample adding groove (130) and the detection hole (120). The sample adding groove (130) is formed along the upper surface of the chip substrate (100) and has a long and flat groove shape. The support assembly (200) includes a support ring (210) mounted on the chip substrate (100) and used for supporting the sealing film (110).

2. The microfluidic chip according to claim 1, wherein, The support assembly (200) includes a support ring (210) mounted on the chip substrate (100) and used for supporting the sealing film (110).

3. The microfluidic chip of claim 1, wherein, The support assembly (200) includes a support ring (210) mounted on the chip substrate (100) and used for supporting the sealing film (110).

4. The microfluidic chip of claim 1, wherein, The support assembly (200) includes a support ring (210) mounted on the chip substrate (100) and used for supporting the sealing film (110).

5. The microfluidic chip of claim 4, wherein, The gap (230) is radially outwardly expanding, and the inner port distance of the gap (230) is less than the outer port distance of the gap (230).

6. The microfluidic chip according to any one of claims 2-4, wherein, The top surface of the support ring (210) is fixedly connected with the sealing membrane (110).

7. The microfluidic chip according to claim 6, wherein, The support assembly (200) further comprises a plurality of support pieces (240) arranged uniformly along the extending direction of the sample adding groove (130), one end of the support piece (240) is fixedly connected to the chip substrate (100), and the other end of the support piece (240) is fixedly connected to the sealing membrane (110).

8. The microfluidic chip of claim 6, wherein, The support assembly (200) further comprises a support piece (240) arranged along the extending direction of the sample adding groove (130), the support piece (240) is arranged in an arc shape and located at the middle of the sample adding groove (130).

9. The microfluidic chip of claim 1, wherein, The sample adding groove (130) is arranged in an arc shape starting from the sample adding port (150) and extending along the circumferential direction of the chip substrate (100).

10. The microfluidic chip of claim 1, wherein, The sealing membrane (110) is provided with a quantitative line (250) for indicating the amount of sample to be added.

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