Protein enrichment device

Abstract

The utility model provides a protein enrichment device which comprises a heat preservation shell and a test tube rack, the test tube rack is arranged in the heat preservation shell, and a gap is formed between the outer wall of the test tube rack and the inner wall of the heat preservation shell; a plurality of test tube channels for placing test tubes are transversely arranged in the test tube rack; and a test tube rack body is arranged among the test tube channels and is made of a heat-conducting material. According to the device provided by the utility model, the separation between the target protein and the target protein antibody beads caused by liquid dripping is reduced, so that the enrichment efficiency of the protein is effectively improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological storage container technical field, concretely relates to a protein enrichment device. BACKGROUND

[0002] Protein enrichment is to selectively separate and concentrate specific proteins or protein populations with specific properties from complex biological samples to effectively study the properties of these target proteins. In the prior art, the antibody is coupled by using small-sized beads (Beads) such as agarose beads or magnetic beads; specifically, by mixing and incubating these Beads with the extracted total protein solution, the antibody on the Beads is combined with the target protein, and then the target protein is enriched by collecting the Beads.

[0003] Currently, affinity chromatography columns with sieve plates and homogenizers are usually used, that is, the sieve plate structure is used to increase the contact area of Beads antibody and target protein, and the combination of target protein and Beads is promoted by vertical reciprocating rotation to improve the combination efficiency of Beads and target protein.

[0004] However, due to the high price of empty column tubes and their related reagents, and the fact that empty column tubes cannot be reused, the experimental cost is high; and when the homogenizer is vertically reciprocated, although it is beneficial to the combination of target protein and Beads, the reciprocating dripping of liquid will impact the combination rate of target protein and Beads, causing some Beads that have combined target protein to separate, affecting the enrichment efficiency.

[0005] Therefore, how to efficiently, conveniently and low-costly realize the enrichment of target protein has become a technical problem to be solved in the field. UTILITY MODEL CONTENT

[0006] In view of the above-mentioned shortcomings of the prior art, the purpose of the utility model is to provide a protein enrichment device to solve the problem that the existing protein enrichment device is affected by the reciprocating dripping of liquid to impact the combination rate of target protein and Beads, resulting in reduced enrichment efficiency.

[0007] To achieve the above-mentioned purpose and other related purposes, the utility model provides a protein enrichment device, which comprises: a heat preservation shell, the shell is made of heat preservation material, which can realize heat preservation of the internal space; a test tube rack is placed in the interior of the heat preservation shell, and a gap for placing a cold / heat source is provided between the outer wall of the test tube rack and the inner wall of the heat preservation shell; a plurality of test tube channels for placing test tubes are horizontally arranged in the interior of the test tube rack; the test tube channels are connected by a test tube rack body, and the material of the body is heat conductive material.

[0008] In an embodiment of the present application, one end of each of the test tubes is open at a side end surface of the test tube rack, and the other end is closed inside the test tube rack to form an open-ended accommodation space; the inner diameter of the accommodation space is larger than the outer diameter of the test tube, and the channel depth is greater than the length of the test tube.

[0009] In an embodiment of the present application, each of the test tube channels is arranged in parallel in the test tube rack, and the spacing between adjacent two test tube channels is the same.

[0010] In an embodiment of the present application, the test tube channels are arranged at an inclined angle in the test tube rack.

[0011] In an embodiment of the present application, the inclined angle of the test tube channels is any angle in the range of 20-30 degrees.

[0012] In an embodiment of the present application, the test tube rack is provided with an extension at the opening of the test tube channel, which protrudes from the side wall of the test tube rack, and the upper end surface of the extension is semicircular arc-shaped and seamlessly connected with the side wall of the test tube channel to extend the test tube channel to the outside of the test tube rack.

[0013] In an embodiment of the present application, the test tube channels are arranged in a bidirectional symmetry in the test tube rack, and a partition is arranged between the two oppositely arranged test tube channels.

[0014] In an embodiment of the present application, a resilient ring is fixed inside the opening of each of the test tube channels, and a plurality of open slits are arranged on the inner ring of the resilient ring in the circumferential direction.

[0015] In an embodiment of the present application, the test tube rack is adhesively or detachably connected with the bottom of the heat preservation shell.

[0016] In an embodiment of the present application, a plurality of channel partitions are arranged in the test tube rack; the diameters and / or lengths of the test tube channels in different channel partitions are different.

[0017] In an embodiment of the present application, an anti-skid layer is arranged on the outer bottom surface of the heat preservation shell.

[0018] As described above, the protein enrichment device of the present application, by arranging the test tube channels transversely inside the test tube rack, allows the test tubes to be placed in the test tube rack in a nearly horizontal manner, greatly reducing the liquid drop distance, thereby weakening the impact when the liquid drops, effectively reducing the separation between the target protein and the Beads caused by the liquid drop, and effectively improving the protein enrichment efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 The structure schematic view (front view) of the protein enrichment device in an embodiment of the present application is shown.

[0020] Figure 2 A structural schematic view (rear view) of the protein enrichment device is shown in an embodiment of the present application.

[0021] Figure 3 A side view of the test tube rack is shown in an embodiment of the present application.

[0022] Figure 4 A schematic view of the test tube channel is shown in an embodiment of the present application.

[0023] Figure 5 A schematic view of the test tube channel is shown in another embodiment of the present application.

[0024] Figure 6 A schematic view of the channel partition is shown in an embodiment of the present application.

[0025] Element number explanation

[0026] 10 - protein enrichment device; 100 - incubation box shell; 200 - test tube rack; 210 - test tube channel; 220 - test tube rack body; 230 - extension; 240 - partition; 250 - channel partition; 300 - test tube. DETAILED DESCRIPTION

[0027] The embodiments of the present application will be described in detail with specific examples. Those skilled in the art can easily understand other advantages and effects of the present application from the content disclosed in the specification. The present application can also be implemented or applied in other different embodiments, and each detail in the specification can be modified or changed based on different viewpoints and applications without departing from the spirit of the present application. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0028] It should be noted that the drawings provided in the following embodiments only schematically illustrate the basic concept of the present application, and only the components related to the present application are shown in the drawings, not the number, shape and size of the components when actually implemented. The shape, number and ratio of each component when actually implemented can be arbitrarily changed, and the layout pattern of the components can also be more complex.

[0029] It should be noted that when a component is referred to as being "connected" to another component, it can be directly connected to the other component or there can be a middle component. When a component is referred to as being "disposed on" another component, it can be directly disposed on the other component or there can be a middle component.

[0030] In view of the fact that the existing protein enrichment device is affected by the impact of the reciprocating dripping of liquid on the binding rate of the target protein and the beads, resulting in reduced enrichment efficiency and other problems, the protein enrichment device provided by the application greatly reduces the impact force of liquid dripping by laterally arranging test tube channels in the test tube rack, effectively reduces the impact of the impact force on the binding rate, and thus improves the protein binding enrichment efficiency.

[0031] Referring to Figures 1 to 3 , which shows the structural schematic diagram of the protein enrichment device provided by the application in an embodiment; wherein, Figure 1 is a front view of the protein enrichment device, Figure 2 is a rear view of the protein enrichment device, Figure 3 is a side view of the test tube rack.

[0032] As Figure 1 and Figure 2 show, the protein enrichment device 10 comprises a heat preservation shell 100 and a test tube rack 200.

[0033] The heat preservation shell 100 has an accommodation space inside, and the shell is made of heat preservation material to achieve heat preservation of the internal space of the shell; for example, the heat preservation shell 100 is made of polyurethane foam, polystyrene foam or other existing heat preservation materials.

[0034] The height of the heat preservation shell 100 is not less than the height of the test tube rack 200, so that the test tube rack 200 is completely accommodated in the internal space of the heat preservation shell 100, to ensure that the entire test tube rack 200 is in the same temperature environment, thereby achieving better heat preservation effect.

[0035] The test tube rack 200 is placed in the internal space of the heat preservation shell 100, and an accommodation gap is formed between the outer wall of the test tube rack 200 and the inner wall of the heat preservation shell, for accommodating a cold / heat source, such as an ice bag / heating bag, to heat or cool the test tube rack 200, so as to ensure that the test tube rack 200 is in a uniform temperature environment.

[0036] The test tube rack 200 is provided with a plurality of laterally arranged test tube channels 210, each of which is used for laterally placing a test tube 300 for protein enrichment; the test tube channels 210 are connected by a test tube rack body 220, and the material of the body is a material with high heat conduction efficiency, such as copper, aluminum or other metal materials, to ensure that the temperature around each test tube 300 is close to the temperature around the test tube rack 200, and to realize heating or cooling of the placed test tube 300.

[0037] In some alternative embodiments, a flow guide channel (not shown) is further arranged around each of the test tube channels 210 in the test tube rack body 220, and each of the flow guide channels is connected to each other to form a network of interconnected flow guide channels for flowing refrigerant / heat medium in each of the flow guide channels, so that cold or heat energy can be quickly transferred when the refrigerant / heat medium flows in the flow guide channels, thereby achieving rapid cooling or heating of the test tube rack.

[0038] In use, after the test tube rack is pre-cooled / pre-heated, the test tubes are inserted into each of the test tube channels of the test tube rack, and the test tube rack is placed in the insulation shell to achieve insulation of each of the test tubes in the test tube rack, so as to achieve the protein enrichment process in the insulation environment.

[0039] The protein enrichment device provided by the embodiment greatly reduces the falling distance of the liquid, thereby weakening the impact of the liquid when falling, by arranging each test tube channel in a transverse direction and placing the test tubes in the test tube rack in a nearly horizontal manner. Therefore, compared with the vertical arrangement of the test tube channel in the prior art, the impact of the liquid falling on the influence of the target protein and the Beads combination can be reduced, thereby effectively improving the protein enrichment efficiency.

[0040] In some specific embodiments, one end of each of the test tubes is open at one side end surface of the test tube rack, and the other end is closed inside the test tube rack to form an open-ended accommodation space; the inner diameter of the accommodation space is larger than the outer diameter of the test tube, and the channel depth is greater than the length of the test tube, so as to facilitate the insertion and removal of the test tube.

[0041] Further, each of the test tube channels is arranged in parallel in the test tube rack, and the spacing between two adjacent test tube channels is the same, so as to ensure that the temperature around each test tube is the same or similar.

[0042] During the protein enrichment process, the test tubes need to be shaken horizontally. In order to avoid each test tube from being shaken out of or away from the test tube channel during the horizontal shaking process, in some specific embodiments, as shown in Figure 4 The test tube channel 210 is arranged at an inclined angle in the test tube rack 200, so that the test tube is placed horizontally after being inserted, thereby further ensuring the stability of the test tube during shaking.

[0043] More specifically, the inclined angle of the test tube channel is any angle in the range of 20-30 degrees.

[0044] Further, in order to ensure that the test tube wall can be in complete contact with the side wall of the test tube channel when the test tube is inclined, as shown in Figure 4 The test tube rack is provided with an extension 230 at the opening of the test tube channel.

[0045] Specifically, the extension 230 protrudes from the side wall of the test tube rack 200, and the upper surface of the extension 230 is designed as a semi-circular arc-shaped platform, so that the upper surface of the extension 230 can be seamlessly connected with the side wall of the test tube channel 210, forming a portion of the test tube channel 210 extending outward from the test tube rack 200. This ensures that the outer surface of the test tube is fully covered when the test tube is tilted, maintaining the stability of the test tube while further improving the mixing efficiency, thereby enhancing the protein enrichment effect.

[0046] In one optional embodiment, the test tube channels are arranged symmetrically in both directions within the test tube rack, such as... Figure 5 As shown, each of the test tube channels is arranged in a mirror image on both sides of the test tube rack, which improves the stability and balance of the test tube rack and increases its utilization rate. Furthermore, a partition 240 is provided between the two test tube channels 210 arranged in opposite directions to separate the two test tube channels 210, preventing test tubes in different channels from colliding directly when shaken, thereby reducing the risk of test tube breakage and protecting the samples inside the test tubes from damage.

[0047] It should be noted that in some other embodiments, the test tube channels are arranged in a crisscross pattern on both sides of the test tube rack; specifically, the test tube channels arranged on the first side are spaced apart, and the test tube channels arranged on the second side are filled in the gaps between the test tube channels arranged on the first side. This not only enhances the stability of the test tubes during shaking, but also effectively improves the space efficiency of the test tube rack, making it easier for experimenters to insert and remove test tubes from different directions and angles, thus improving the convenience of operation.

[0048] In one optional embodiment, to achieve compatibility with test tubes of different sizes, the test tube rack is provided with several channel partitions 250; the test tube channels in different channel partitions 250 have different diameters and / or extension lengths to accommodate test tubes of different sizes, thereby improving the versatility of the protein enrichment device. More specifically, the test tube rack can be compatible with test tubes of different sizes ranging from 200 μL to 50 mL.

[0049] In one optional embodiment, to better accommodate test tubes of different sizes (diameter or length), an elastic ring is fixed inside the opening of each test tube channel; wherein, the inner ring of the elastic ring has several openings arranged circumferentially, and when inserting the test tube, the test tube is inserted into the test tube channel after passing through the inner ring of the elastic ring, so that after insertion, the inner ring wall of the elastic ring will tightly abut against the test tube wall, thereby supporting the test tube, ensuring the stability of the test tube in the test tube channel, and preventing the test tube from being displaced under shaking or other external forces; and, the elastic properties of the elastic ring enable it to adapt to test tubes of different diameters or lengths, while providing uniform support force after the test tube is inserted.

[0050] In an alternative embodiment, the test tube rack is a square housing and is vertically placed inside the incubation housing.

[0051] In an alternative embodiment, the test tube rack is adhesively or detachably connected to the bottom of the incubation housing, and the relative positions of the two are fixed when the test tube rack is placed in the incubation housing, so that the positions of the two do not deviate when the protein enrichment device is shaken. When the test tube is replaced, the test tube rack can be taken out of the incubation housing, achieving the effects of reusability and convenient replacement, effectively improving the service life of the protein enrichment device and reducing the use cost.

[0052] In an alternative embodiment, the outer bottom surface of the incubation housing is provided with an anti-skid layer to prevent the incubation housing from sliding when it is horizontally shaken on a shaking table.

[0053] For ease of understanding, the use of the above protein enrichment device is described below by taking the protein induced by the PET28a(+) prokaryotic expression vector as an example.

[0054] Taking this embodiment as an example, the specific implementation process is as follows:

[0055] Step 1: After inducing the expression of the prokaryotic recombinant protein (220 rpm, 3 h, 37°C), collect the bacterial cells (5000 g, 10 min, 4°C) and discard the supernatant;

[0056] Step 2: Add 1 mL of protein extraction solution to suspend the bacterial solution, and use an ultrasonic cell disruptor to break the cells;

[0057] Step 3: Centrifuge (13000 g, 30 min, 4°C) and take the supernatant;

[0058] Step 4: 100 μL His-tag Beads are added to 1 mL of non-denaturing lysis solution, balanced for 3 times, centrifuged (2500 g, 2 min, 4°C), and the supernatant is discarded;

[0059] Step 5: Add 1 mL of protein sample (c) to each test tube, place the test tubes evenly in the protein enrichment device, and ensure that the interval distance of each test tube in the device is similar; horizontally shake the protein enrichment device (60 rpm, 2 h), centrifuge (2500 g, 2 min, 4°C), and discard the supernatant;

[0060] Step 6: Add 500 μL of 10 mM non-denaturing washing solution to resuspend the gel, centrifuge (2500 g, 2 min, 4°C), and discard the supernatant.

[0061] Step 7, add 500 μL 50 mM non-denaturing eluent, centrifuge (2500 g, 2 min, 4°C), collect the supernatant;

[0062] Step 8, detect the target protein sample collected in the elution.

[0063] The technical features of the above-described embodiments can be combined in any manner. For the sake of brevity, not all possible combinations of the technical features in the above-described embodiments are described, however, as long as the combination of the technical features does not result in a contradiction, it shall be considered within the scope of the present disclosure. When the technical features in different embodiments are embodied in the same drawing, it can be considered that the drawing also discloses the combination of the embodiments involved.

[0064] The above-described embodiments only exemplarily illustrate the principles and effects of the present application, and are not used to limit the present application. Any person skilled in the art can modify or change the above-described embodiments without departing from the spirit and scope of the present application. Therefore, all equivalent modifications or changes made by those skilled in the art without departing from the spirit and technical concept disclosed by the present application shall be covered by the claims of the present application.

Claims

1. A protein enrichment device, characterized by, The application relates to a heat-preservation test tube rack. The heat-preservation test tube rack comprises a heat-preservation shell, a test tube rack arranged in the heat-preservation shell, and a heat-conducting body arranged between the test tube rack and the heat-preservation shell. Each test tube channel is open at one end and closed at the other end to form an open-end accommodation space.

2. The protein enrichment device of claim 1, wherein, The test tube channels are arranged in parallel in the test tube rack, and the distance between two adjacent test tube channels is the same.

3. The protein enrichment device of claim 1, wherein, The test tube channels are arranged at an inclined angle in the test tube rack.

4. The protein enrichment device of claim 1, wherein, The inclined angle of the test tube channels is any angle between 20 and 30 degrees.

5. The protein enrichment device of claim 4, wherein, The test tube rack is provided with an extension part at the opening of the test tube channel, which protrudes from the side wall of the test tube rack, and the upper end surface of the extension part is semicircular and seamlessly connected with the side wall of the test tube channel, so that the test tube channel extends to the outside of the test tube rack.

6. The protein enrichment device of claim 4 or 5, wherein, The test tube channels are arranged in a bidirectional symmetry in the test tube rack, and a partition part is arranged between two oppositely arranged test tube channels.

7. The protein enrichment device of claim 1, wherein, An elastic ring is fixed in the opening of each test tube channel, and a plurality of opening slits are arranged on the inner ring of the elastic ring in the circumferential direction.

8. The protein enrichment device of claim 1, wherein, The test tube rack and the bottom of the heat-preservation shell are adhesively connected or detachably connected.

9. The protein enrichment device of claim 1, wherein, A plurality of channel partitions are arranged in the test tube rack, and the diameters and / or lengths of the test tube channels in different channel partitions are different.

10. The protein enrichment device of claim 1, wherein, ​

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