Disposable sterile sample incubator
By using modularly designed aseptic isolation tubes and filter membrane structures, the problems of microbial invasion and resource waste in existing culture devices have been solved. This enables the rapid establishment of a sterile environment and flexible adjustment of various experimental methods, improving experimental accuracy and reducing costs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-23
- Publication Date
- 2026-04-10
AI Technical Summary
Existing culture devices are prone to microbial invasion during gas exchange, filter membranes are easily clogged and have low replacement efficiency, and the device's functional modules cannot be quickly adjusted, leading to sample contamination and resource waste.
A disposable sterile sample incubator was designed, which adopts a modular sterile isolation tube and filter membrane structure. Gas filtration and sample isolation are achieved through the connection of bent tubes and threaded sleeves. Combined with a sealing plate and a partition plate to control gas flow, a sterile environment can be quickly established and samples can be protected.
It effectively prevents microbial contamination, improves experimental accuracy, reduces experimental costs, and allows for flexible adjustment of various experimental methods.
Smart Images
Figure CN224105818U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to sample culture technical field, concretely is disposable sterile sample incubator. BACKGROUND
[0002] In the field of biological medicine, food detection and environmental monitoring, sample culture is the core link of microbial experiment, and traditional culture device (such as thermostat incubator, anaerobic culture tank) adopts closed or semi-closed structure, and maintains sterile environment through physical isolation or chemical reagent.
[0003] In the prior art, the conventional incubator relies on the sealing property to block the external microorganism, but when the door is frequently opened and closed or gas exchange (such as oxygen / carbon dioxide adjustment) is carried out, the external microorganism is easy to invade through the gap, leading to sample pollution, and the influence on sensitive samples such as anaerobic bacteria and sterile body fluid is significant, and the existing device usually adopts one-way valve or simple filter membrane to realize gas flow, but when the filter membrane aperture is uneven or the valve sealing property is insufficient, microorganisms can still enter the culture area along with the airflow, and the filter membrane is easy to be blocked after long-term use, but due to the use position of the filter membrane, the efficiency of replacing the filter membrane is extremely low.
[0004] The gas treatment unit and the culture cavity of the existing device are usually integrated, so that the whole device needs to be repaired or replaced when damaged, and the functional modules cannot be quickly adjusted according to the experimental requirements, resulting in resource waste and increasing the experimental cost. UTILITY MODEL CONTENTS
[0005] In view of the above technical problems, the utility model provides the following technical scheme:
[0006] The disposable sterile sample incubator comprises a bed body, a partition plate is fixedly installed in the bed body, the partition plate divides the bed body into two parts, one part is a sample storage chamber for storing samples for culture, and the other part is a gas exchange chamber, a ventilation pipe is fixedly installed on one side of the bed body, one end of the ventilation pipe is arranged in the gas exchange chamber, and the other end of the ventilation pipe is arranged outside the bed body, and a sterile isolation pipe can be further arranged on the ventilation pipe of the bed body.
[0007] Further, the sterile isolation pipe comprises an elbow pipe, a threaded sleeve is rotatably installed at one end of the elbow pipe, a cover switch is threadedly installed at the other end of the threaded sleeve, the threaded sleeve is connected with the ventilation pipe through threads, a plurality of air filters for filtering microorganisms are fixedly installed in the elbow pipe, a sealing ring is fixedly installed on the inner wall of the threaded sleeve, a sealing film is arranged in the sealing ring, the material of the sealing film is polyethylene material, an outer ring filter screen is fixedly installed on the inner wall of the one end of the elbow pipe provided with the cover switch, an inner rotating filter screen is rotatably installed in the outer ring filter screen, the inner rotating filter screen is fixedly connected with the cover switch, and the hole positions of the inner rotating filter screen and the outer ring filter screen are staggered in the initial state.
[0008] Further, the bed body is slidably provided with a sealing plate, one end of the sealing plate is provided with a pulling handle, a plurality of limiting grooves are arranged on the sealing plate, and the sealing plate is a sealing part of a sample storage chamber in the bed body.
[0009] Further, the partition plate is fixedly provided with a clamping strip, and the clamping strip is made of elastic plastic material.
[0010] Further, two protrusions are arranged at the end of the clamping strip away from the partition plate, the protrusions are matched with the limiting grooves on the sealing plate, and the position of the sealing plate is limited when the protrusions are matched with the limiting grooves.
[0011] Further, a plurality of air holes for filtering dust are arranged at the end of the partition plate away from the liquid guide slope, and a partition sliding plate is slidably arranged on the partition plate, the air holes are closed by the partition sliding plate in an initial state, the air holes are connecting channels of a gas exchange chamber and the sample storage chamber, and the partition sliding plate is a switch of the connecting channels of the gas exchange chamber and the sample storage chamber.
[0012] Further, a sealing cover is threadedly arranged at the end of the air pipe outside the bed body, the sealing cover is used for sealing the air pipe and the gas exchange chamber, and a sealing cover for crushing is fixedly arranged in the air pipe.
[0013] The beneficial effects of the present application compared with the prior art are as follows: (1) the device is provided with a sterile isolation pipe on one side of the bed body, and the air filter membrane in the isolation pipe is used for filtering microorganisms, so that the conditions of gas circulation during sample cultivation are met, the entry of microorganisms during sample cultivation is reduced, the process of sample cultivation is prevented from being changed by microorganisms, and the experimental accuracy is improved; (2) the sterile isolation pipe and the bed body are modularly designed, so that the device has multiple experimental modes, and the experimental cost of the laboratory is reduced. BRIEF DESCRIPTION OF DRAWINGS
[0014] Figure 1 It is a whole structure schematic view of the present application.
[0015] Figure 2 It is a structure schematic view of the bed body, the sealing plate and the partition plate after being cut.
[0016] Figure 3 It is a structure schematic view of the sealing plate, the partition plate and the rubber pad after being cut.
[0017] Figure 4 It is a structure schematic view of the sterile isolation pipe.
[0018] Figure 5 It is a structure schematic view of the elbow pipe, the threaded sleeve, the inner rotating filter screen, the sealing cover switch and the outer ring filter screen after being cut.
[0019] Figure 6 This is a cross-sectional structural diagram of the bent pipe, threaded sleeve, sealing switch, and outer ring filter screen of this utility model.
[0020] Figure 7 This is a cross-sectional structural diagram of the outer ring filter, inner rotating filter, and sealing switch of this utility model.
[0021] Figure 8 This is a schematic diagram of the aseptic isolation tube structure of this utility model after it is connected to the bed.
[0022] Figure 9 This is a cross-sectional structural diagram of the bed, air pipe, bend, and threaded sleeve of this utility model.
[0023] Reference numerals: 101-Bed; 102-Sealing plate; 103-Positioning strip; 1031-Protrusion; 104-Divider plate; 105-Divider slide plate; 106-Ventilation hole; 107-Rubber pad; 108-Drainage screen; 109-Liquid guiding slope; 110-Ventilation pipe; 111-Spiked claw; 112-Sealing cover; 201-Bend; 202-Threaded sleeve; 203-Rubber ring; 204-Sealing ring; 205-Air filter membrane; 206-Outer ring filter; 207-Inner rotating filter; 208-Sealing switch. Detailed Implementation
[0024] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.
[0025] like Figure 1 and Figure 2 As shown, a disposable sterile sample incubator includes a bed 101, which is mainly a storage container. The bed 101 is generally made of glass, plastic (such as polystyrene), etc., and is usually transparent for easy observation by the experimenter. A partition 104 is fixedly installed inside the bed 101, which divides the bed 101 into two parts: a sample storage chamber for storing samples for incubation and a gas exchange chamber. The sample storage chamber occupies nine-tenths of the space of the bed 101, and the gas exchange chamber occupies one-tenth of the space of the bed 101.
[0026] like Figures 1 to 3As shown, the bed body 101 is slidingly mounted with a sealing plate 102, one end of the sealing plate 102 is provided with a pull handle, the edge of the sealing plate 102 in contact with the bed body 101 is provided with a rubber strip, so as to enhance the sealing of the bed body 101 and the sealing plate 102, the partition plate 104 is provided with a rubber pad 107, the rubber pad 107 can be in contact with the sealing plate 102, the rubber pad 107 is used to close the gap between the sealing plate 102 and the partition plate 104, the sealing plate 102 is a sealing part of the sample storage chamber in the bed body 101, when the sealing plate 102 and the rubber pad 107 on the partition plate 104 are in close contact, the sample storage chamber on the bed body 101 will be sealed, so as to culture some special samples by anaerobic method.
[0027] As shown in the drawings, Figures 1 to 3 The bottom of the sample storage chamber of the bed body 101 is provided with a liquid leakage net 108, the sample storage chamber is provided with a liquid collection groove, the liquid leakage net 108 is arranged above the collection groove for filtering liquid, and an inclined liquid guide slope 109 is arranged, the lowest end of the liquid guide slope 109 is arranged on the side close to the liquid leakage net 108, so that the sample storage chamber can collect liquid when storing some samples which are easy to seep liquid and need to separate liquid and solid in time, in this embodiment, the liquid leakage net 108 and the collection groove can be removed, so as to change the application range of the product and reduce the production cost or selling price of the product.
[0028] As shown in the drawings, Figures 1 to 3 The sealing plate 102 is provided with a plurality of limiting grooves, the partition plate 104 is fixedly mounted with a clamping strip 103, the clamping strip 103 is made of elastic plastic material, the end of the clamping strip 103 away from the partition plate 104 is elastic, according to the material of the clamping strip 103, the end of the clamping strip 103 away from the partition plate 104 can be displaced at a certain angle, the end of the clamping strip 103 away from the partition plate 104 is provided with two protrusions 1031, the protrusions 1031 can be engaged with the limiting grooves on the sealing plate 102, the position of the sealing plate 102 on the bed body 101 is limited by the clamping strip 103 or the protrusions 1031, when the protrusions 1031 are engaged with the limiting grooves, the movement of the sealing plate 102 is limited, when the user pulls the end of the clamping strip 103 away from the partition plate 104, the protrusions 1031 on the clamping strip 103 are out of contact with the limiting grooves, at this time, the user can pull the sealing plate 102 to slide on the bed body 101, so as to open or close the sample storage chamber.
[0029] As shown in the drawings, Figures 1 to 3 And Figure 9As shown, the partition plate 104 is provided with a plurality of air holes 106 for filtering dust at one end away from the liquid guide slope 109, and a partition sliding plate 105 is slidingly installed on the partition plate 104, the partition sliding plate 105 closes the air holes 106 in the initial state, and the contact surface of the partition sliding plate 105 with the partition plate 104 is provided with rubber material to improve the sealing effect, the air holes 106 are the connecting channels of the gas exchange chamber and the sample storage chamber, and the partition sliding plate 105 is the switch of the connecting channels of the gas exchange chamber and the sample storage chamber, and after the partition sliding plate 105 closes the air holes 106, the gas exchange chamber and the sample storage chamber are separated.
[0030] As shown in the drawings, Figures 1 to 3 With Figure 9 As shown, the bed body 101 is fixedly installed with an air pipe 110 at one side, one end of the air pipe 110 is arranged in the gas exchange chamber, and the other end of the air pipe 110 is arranged outside the bed body 101, a closing cover 112 is threadedly installed at the end of the air pipe 110 outside the bed body 101, the closing cover 112 is used to close the air pipe 110 and the gas exchange chamber, and a closing cover 112 for crushing is fixedly installed in the air pipe 110, the closing cover 112 is used when the device is connected with external equipment, and when the user does not need to close the sample storage chamber, the closing cover 112 can be unscrewed from the air pipe 110, so that the closing cover 112 is separated from the air pipe 110, so that the gas can enter the gas exchange chamber naturally, and then the user pulls the partition sliding plate 105 to open the connecting channel air holes 106 between the gas exchange chamber and the sample storage chamber, so that the oxygen in the gas exchange chamber enters the sample storage chamber, so that the oxygen in the sample storage chamber is replaced, and in some experiments of periodically replacing the gas in the sample storage chamber, the channel at the air holes 106 can be intermittently closed or opened to achieve the effect, and when the device needs to inject a specific gas into the sample storage chamber, the pipeline for injection can be directly connected with the air pipe 110 through screw connection, and then the specific gas is injected, and after completion, the channel at the air holes 106 is closed, and finally the closing cover 112 is connected with the air pipe 110.
[0031] As shown in the drawings, Figures 4 to 6 With Figure 8 , Figure 9 As shown, the air pipe 110 of the bed body 101 can also be provided with a sterile isolation pipe, the sterile isolation pipe comprises an elbow pipe 201, one end of the elbow pipe 201 is rotatably installed with a threaded sleeve 202, the other end of the threaded sleeve 202 is threadedly installed with a cover switch 208, and the threaded sleeve 202 is connected with the air pipe 110 through screw connection.
[0032] As shown in the drawings, Figures 4 to 6 WithFigure 9 As shown in the figure, the inner wall of the threaded sleeve 202 is fixedly provided with a sealing ring 204, and the sealing ring 204 is provided with a sealing film. The material of the sealing film is polyethylene material, and the sealing film can be pierced by the sharp claw 111 in the breather pipe 110. Before being pierced, the sealing film is a device for isolating the parts in the elbow pipe 201 from the outside, so as to protect the parts in the elbow pipe 201 from being contaminated by bacteria. The threaded sleeve 202 is fixedly provided with a rubber ring 203. The rubber ring 203 is in contact with the breather pipe 110 when the sterile isolation pipe is installed, so as to enhance the sealing performance of the breather pipe 110 after being connected with the threaded sleeve 202.
[0033] As shown in the figure, Figure 5 , Figure 6 , Figure 9 As shown in the figure, the elbow pipe 201 is fixedly provided with a plurality of air filter membranes 205 for filtering microorganisms. Generally, two or more air filter membranes 205 are arranged in the elbow pipe 201, and the number of air filter membranes 205 in the elbow pipe 201 can be set according to different product models. Generally, the more the number of air filter membranes 205 in the elbow pipe 201, the longer the use time of the whole sterile isolation pipe will be. The air filter membrane 205 is composed of polypropylene (PP) or polyester non-woven fabric, and other mature filter membranes on the market can also be used for replacement.
[0034] As shown in the figure, Figures 4 to 9 As shown in the figure, the elbow pipe 201 is provided with an outer ring filter screen 206 fixedly arranged on one end of the inner wall of the cover switch 208. The inner rotating filter screen 207 is rotatably arranged in the outer ring filter screen 206. The inner rotating filter screen 207 is fixedly connected with the cover switch 208. In the initial state, the inner rotating filter screen 207 is offset from the hole position of the outer ring filter screen 206, as shown in the figure, Figure 7 As shown in the figure, the inner rotating filter screen 207 and the outer ring filter screen 206 isolate the inside and outside of the elbow pipe 201 in the unused state. Only when the elbow pipe 201 is in the used state and the threaded sleeve 202 is connected with the breather pipe 110, the cover switch 208 is rotated, and the cover switch 208 rotates with the inner rotating filter screen 207. In the range of 20 degrees to 25 degrees, the channel between the inner rotating filter screen 207 and the outer ring filter screen 206 is in the maximum state. In the specific use process, after the channel between the inner rotating filter screen 207 and the outer ring filter screen 206 is opened, the external normal gas enters the elbow pipe 201, and then passes through the plurality of air filter membranes 205 in the elbow pipe 201 for filtering, so as to reduce the microorganisms in the gas. The gas enters the gas exchange chamber through the breather hole 106, and then enters the sample storage chamber. In this way, the sample storage chamber can provide sterile oxygen. The air filter membrane 205 can also prevent the microorganisms generated in the sample storage chamber from escaping from the device to affect the external storage environment.
[0035] The device is used in three states: the first is to culture samples using the oxygen-free method; the second is to culture samples using the normal method, in which the samples are in contact with external oxygen; and the third is to culture samples using the sterile method, in which the samples are in a sterile environment and the oxygen they contact is filtered. Only the third method requires the use of a sterile isolation tube, so the sterile isolation tube is made separately from the bed 101 to reduce experimental costs. It should be noted that the device is a disposable product, and the bed 101 and the sterile isolation tube are stored separately in independent plastic packages. In particular, the sterile isolation tube requires a more airtight packaging material. The service life of the sterile isolation tube is limited by the service life of the air filter 205, and the service life of the air filter 205 can be changed by using different materials to adapt to different product use locations.
[0036] The first and second methods of using the device are to place the sample directly in the sample storage chamber after opening the sealing plate 102, and then push the sealing plate 102 into contact with the rubber pad 107 to close the sample storage chamber. The change in ventilation is achieved by manually opening the closure cover 112 and then pulling or closing the partition slide 105. The general use method is to open the partition slide 105 periodically to ventilate the sample, and then close the partition slide 105 when the ventilation time is reached to continue to close the sample in the sample storage chamber. It should be noted that when the closure cover 112 is detached from the ventilation pipe 110, the partition slide 105 controls the switch for the entry and exit of gas. In this case, the gas exchange chamber may contain microorganisms, so it is not suitable to install a sterile isolation tube for the third use method. Therefore, the third use method requires the installation of a sterile isolation tube for sample culture from the beginning.
[0037] The third use method needs to take the sterile isolation tube out of the plastic package, and then install the threaded sleeve 202 on the air pipe 110. When the threaded sleeve 202 is screwed on the air pipe 110, the sharp claw 111 will slowly pierce the sealing film on the sealing ring 204. When the sharp claw 111 pierces the sealing film, the sealing film is rotated with the threaded sleeve 202 and approaches the air pipe 110, so the sealing film will be certainly pressed and broken by the sharp claw 111. After the one end of the elbow pipe 201 is opened, the preparation of the sterile sample culture of the device is completed. The device in this state is a complete device. Then the sealing plate 102 can be pulled, the sample is put into the sample storage chamber, and then the convex block 1031 is used to fix the sealing plate 102. Then the separation slide plate 105 is pulled to open the channel between the gas exchange chamber and the sample storage chamber. Finally, the cover switch 208 is rotated to open the channel between the outer ring filter screen 206 and the inner rotating filter screen 207. The device in this state is in the state during the experiment. During the culture process, the cover switch 208 can also be rotated to close the channel between the outer ring filter screen 206 and the inner rotating filter screen 207. The sample is cultivated in this state, which not only can achieve the condition of gas circulation during sample cultivation, but also can reduce the entry of microorganisms during sample cultivation, prevent the microorganisms from changing the sample cultivation process, and improve the experimental accuracy.
[0038] The above merely describes preferred embodiments of the present application, but is not intended to limit the protection scope of the present application.
Claims
1. A disposable, sterile sample incubator comprising a bed (101), characterized in that: The bed body (101) is fixedly provided with a partition plate (104), which divides the bed body (101) into two parts, one part is a sample storage chamber for storing samples for culture, and the other part is a gas exchange chamber. The sterile isolation pipe comprises a bend pipe (201), one end of the bend pipe (201) is rotatably provided with a threaded sleeve (202), the other end of the threaded sleeve (202) is threadedly provided with a cover switch (208), the threaded sleeve (202) is connected with the ventilation pipe (110) through threads, a plurality of air filters (205) for filtering microorganisms are fixedly arranged in the bend pipe (201), a sealing ring (204) is fixedly arranged on the inner wall of the threaded sleeve (202), a sealing film is arranged in the sealing ring (204), the material of the sealing film is polyethylene material, an outer ring filter screen (206) is fixedly arranged on the inner wall of one end of the bend pipe (201) provided with the cover switch (208), an inner rotating filter screen (207) is rotatably arranged in the outer ring filter screen (206), the inner rotating filter screen (207) is fixedly connected with the cover switch (208), and the inner rotating filter screen (207) is staggered with the hole position of the outer ring filter screen (206) in the initial state.
2. The disposable, sterile sample incubator of claim 1, wherein: The bed body (101) is slidably provided with a sealing plate (102), one end of the sealing plate (102) is provided with a pulling handle, a plurality of limiting grooves are arranged on the sealing plate (102), and the sealing plate (102) is a sealing part of the sample storage chamber in the bed body (101).
3. The disposable, sterile sample incubator of claim 2, wherein: The partition plate (104) is fixedly provided with a clamping strip (103), and the clamping strip (103) is made of elastic plastic material.
4. The disposable, sterile sample incubator of claim 3, wherein: Two protrusions (1031) are arranged on the end of the clamping strip (103) away from the partition plate (104), the protrusions (1031) can be engaged with the limiting grooves on the sealing plate (102), and the position of the sealing plate (102) is limited when the protrusions (1031) are engaged with the limiting grooves.
5. The disposable, sterile sample incubator of claim 1, wherein: A plurality of ventilation holes (106) for filtering dust are arranged on the end of the partition plate (104) away from the liquid guide inclined surface (109), and a partition sliding plate (105) is slidably arranged on the partition plate (104), the partition sliding plate (105) closes the ventilation holes (106) in the initial state, the ventilation holes (106) are connecting channels of the gas exchange chamber and the sample storage chamber, and the partition sliding plate (105) is a switch of the connecting channels of the gas exchange chamber and the sample storage chamber.
6. The disposable, sterile sample incubator of claim 1, wherein: A closing cover (112) is threadedly arranged on the end of the ventilation pipe (110) outside the bed body (101), and the closing cover (112) is used to close the ventilation pipe (110) and the gas exchange chamber. A closing cover (112) is fixedly arranged in the ventilation pipe (110) for crushing.