Rapid separation and culture box for immature embryos of watermelons

Abstract

The utility model relates to the technical field of immature embryo rapid separation and culture, in particular to a watermelon immature embryo rapid separation and culture box which comprises a box body, a fixing plate is fixedly arranged in the box body and divides the box body into a separation chamber and a culture chamber, and a clamp is arranged in the separation chamber and comprises an upper clamping plate and a lower clamping plate. The upper clamping plate and the lower clamping plate are each provided with a plurality of grooves used for containing immature watermelon embryos, a cutting component is further arranged in the separation chamber in a sliding mode, the cutting component at least comprises a first cutter and a plurality of second cutters, and the first cutters and the second cutters are perpendicularly arranged and designed to be provided with arc-shaped cutting edges. Transverse and longitudinal cutting of immature watermelon embryos is achieved, germs are effectively exposed, a plurality of partition plates are arranged in the culture chamber, a drip irrigation component is arranged at the top of the culture chamber, a plurality of culture media are arranged at the bottom of the culture chamber, and exhaust components used for adjusting gas pressure in the box body are fixedly connected to the top of the separation chamber and the side wall of the culture chamber.

Description

Technical Field

[0001] This utility model relates to the field of rapid separation and culture technology of immature embryos, and in particular to a rapid separation and culture box for immature watermelon embryos. Background Technology

[0002] Watermelon in vitro culture technology, as an advanced propagation method, enables rapid propagation and genetic improvement by culturing watermelon tissues or cells under sterile conditions. However, some technical challenges remain in the rapid isolation and culture of immature watermelon embryos, such as low isolation efficiency and difficulty in controlling culture conditions.

[0003] Chinese Patent CN1146928A discloses a method and apparatus for buffered separation of plant seeds. This method uses the buffering effect of the buffer medium in the leaf-type separation device to alleviate the mechanical force exerted on the seeds by the working parts of the equipment, thereby achieving the separation of the seed coat, embryo, and endosperm, maintaining the integrity of the endosperm and embryo, improving the yield of the endosperm and embryo, simplifying the separation process and equipment, and reducing energy consumption. However, when dealing with immature watermelon embryos, the complex cutting operation, the complex embryo removal, and the high air pressure inside the culture box, problems such as low separation efficiency and unstable culture environment occur. In view of this, a rapid separation method and culture box for immature watermelon embryos is provided. Utility Model Content

[0004] The main purpose of this invention is to provide a rapid separation and culture box for immature watermelon embryos, in order to solve the problems of low separation efficiency and unstable culture environment caused by complex cutting operations, complex embryo removal, and high air pressure in the culture box for immature watermelon embryos in related technologies.

[0005] To achieve the above objectives, according to one aspect of the present invention, a rapid separation and cultivation box for immature watermelon embryos is provided, comprising a box body, a fixing plate fixedly disposed inside the box body, the fixing plate dividing the box body into a separation chamber and a cultivation chamber, a clamp for fixing immature watermelon embryos disposed in the separation chamber, and a cutting component slidably disposed in the separation chamber, a plurality of partitions disposed in the cultivation chamber, a drip irrigation component for providing nutrient solution to immature watermelon embryos disposed at the top of the cultivation chamber, a plurality of culture media disposed at the bottom of the cultivation chamber, and an exhaust component fixedly connected to the top of the separation chamber and the side wall of the cultivation chamber;

[0006] The clamp includes an upper clamping plate and a lower clamping plate, and both the upper and lower clamping plates are provided with a plurality of grooves for placing immature watermelon embryos. The cutting component includes at least a first cutter and a plurality of second cutters. The first cutter is used to cut the immature watermelon embryos horizontally, and the second cutters are used to cut the immature watermelon embryos vertically.

[0007] Furthermore, a connecting rod is provided through the upper clamping plate and the lower clamping plate. One end of the connecting rod is rotatably installed in the lower clamping plate, and the other end of the connecting rod is threadedly connected to the upper clamping plate. Several inclined grooves communicating with the groove are opened on one side of the lower clamping plate, and a long groove is also opened on the same side of the lower clamping plate.

[0008] Furthermore, the cutting component also includes a blade holder, with slide rods fixedly connected to both ends of the bottom of the blade holder. One end of the slide rod is slidably mounted on a fixed plate. The first blade is mounted on one side of the blade holder, and the second blade is fixedly connected to the lower side wall of the first blade. The first blade and the second blade are arranged perpendicularly.

[0009] Furthermore, a connecting plate is fixedly connected to the side wall of the first cutter, and the second cutter is also fixedly connected to the side wall of the connecting plate. A receiving plate is snapped into the lower end of the connecting plate, and one end of the receiving plate is inserted into the long groove.

[0010] Furthermore, the partition divides the culture chamber into several culture areas. The partition is a breathable membrane. Each culture area has an insert plate at the bottom. The culture medium is placed on the insert plate. The bottom of the box has a heating chamber and a heat insulation chamber.

[0011] Furthermore, a nutrient solution box is fixedly installed at the bottom of the fixing plate, and the drip irrigation component includes a liquid outlet pipe. A connecting pipe is fixedly connected to the center of the bottom of the liquid outlet pipe. The connecting pipe communicates with the nutrient solution box, and several nozzles are connected to the bottom of the liquid outlet pipe.

[0012] Furthermore, the exhaust component includes a first exhaust pipe and a second exhaust pipe. A first through hole is provided through the first exhaust pipe, and a second through hole is provided through the second exhaust pipe. The diameter of the first through hole is smaller than the diameter of the second through hole, and a float ball is provided in the second through hole. A filter plate is also fixedly provided at the upper end of the second exhaust pipe.

[0013] Furthermore, the box body has through-holes on both sides of the separation chamber, and elastic sealing rings communicating with the through-holes are provided on both sides of the separation chamber.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. In this rapid separation and cultivation box for immature watermelon embryos, the vertical setting of the first and second blades and the arc-shaped blade design enable the horizontal and vertical cutting of the immature watermelon embryos, effectively exposing the embryo. The receiving plate facilitates the collection of the cut embryos and reduces damage to the embryos during the transfer process.

[0016] 2. The watermelon immature embryo rapid separation and culture box is equipped with an exhaust component. The gas pressure inside the culture box is automatically regulated by a float. When the gas pressure increases, the float rises to open the exhaust channel and release excess gas. When the gas pressure is within the normal range, the float moves down to block the exhaust channel, preventing external air from entering, maintaining the stability of the culture environment, and ensuring the normal growth and development of the embryo. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of the rapid separation and culture box for immature watermelon embryos in a preferred embodiment of this utility model;

[0018] Figure 2 This is a schematic diagram of the overall structure of the clamp in a preferred embodiment of the present invention;

[0019] Figure 3 This is a preferred embodiment of the present invention. Figure 2 Enlarged schematic diagram of the structure at point A in the middle;

[0020] Figure 4 This is a schematic diagram of the overall structure of the cutting component in a preferred embodiment of the present invention;

[0021] Figure 5 This is a partial structural diagram of the watermelon immature embryo rapid separation and culture box in a preferred embodiment of the present invention;

[0022] Figure 6 This is a schematic diagram of the planar structure of the culture chamber in a preferred embodiment of the present invention;

[0023] Figure 7 This is a preferred embodiment of the present invention. Figure 6 Enlarged schematic diagram of the structure at point B;

[0024] Figure 8 This is a schematic diagram of the overall structure of the drip irrigation component in a preferred embodiment of the present invention;

[0025] Figure 9 This is a schematic diagram of the overall structure of the exhaust component in a preferred embodiment of the present invention.

[0026] Illustration:

[0027] 1. Box body; 11. Separation chamber; 111. Column hole; 112. Elastic sealing ring; 12. Culture chamber; 13. Fixing plate; 14. Nutrient solution box; 15. Partition plate; 16. Insert plate; 17. Culture medium; 18. Heating chamber; 19. Insulation chamber;

[0028] 2. Fixture; 21. Upper clamping plate; 22. Lower clamping plate; 221. Groove; 222. Inclined groove; 223. Long groove; 23. Connecting rod;

[0029] 3. Cutting component; 31. Tool holder; 32. Slide bar; 33. First tool; 34. Second tool; 35. Connecting plate; 36. Receiving plate;

[0030] 4. Drip irrigation components; 41. Liquid outlet pipe; 42. Connecting pipe; 43. Sprinkler head;

[0031] 5. Exhaust components; 51. First exhaust pipe; 511. First through hole; 52. Second exhaust pipe; 521. Second through hole; 53. Float ball; 54. Filter plate. Detailed Implementation

[0032] To further illustrate the technical means and effects adopted by this utility model in order to achieve the intended utility model purpose, the following detailed description of the specific implementation methods, structure, features and effects of this utility model is provided in conjunction with the accompanying drawings and preferred embodiments.

[0033] Please see Figures 1-9 As shown, the purpose of this embodiment is to provide a rapid separation and cultivation box for immature watermelon embryos, including a box body 1. A fixing plate 13 is fixedly installed inside the box body 1, which divides the box body 1 into a separation chamber 11 and a cultivation chamber 12. A clamp 2 for fixing immature watermelon embryos is installed in the separation chamber 11, and a cutting component 3 is also slidably installed inside the separation chamber 11. Several partitions 15 are installed inside the cultivation chamber 12. A drip irrigation component 4 for providing nutrient solution to the immature watermelon embryos is installed at the top of the cultivation chamber 12. Several culture media 17 are installed at the bottom of the cultivation chamber 12. An exhaust component 5 is fixedly connected to the top of the separation chamber 11 and the side wall of the cultivation chamber 12.

[0034] The clamp 2 includes an upper clamping plate 21 and a lower clamping plate 22, and both the upper clamping plate 21 and the lower clamping plate 22 are provided with a plurality of grooves 221 for placing immature watermelon embryos.

[0035] The cutting component 3 includes at least a first cutter 33 and a plurality of second cutters 34. The first cutter 33 is used to cut the immature watermelon embryos horizontally, and the second cutters 34 are used to cut the immature watermelon embryos vertically.

[0036] A connecting rod 23 is provided through the upper clamping plate 21 and the lower clamping plate 22. One end of the connecting rod 23 is rotatably installed in the lower clamping plate 22, and the other end of the connecting rod 23 is threadedly connected to the upper clamping plate 21. Therefore, when the connecting rods 23 on both sides are rotated at the same time, the upper clamping plate 21 can be moved up or down relative to the lower clamping plate 22. Two nuts are threaded on each connecting rod 23, and the two nuts are located on both sides of the upper clamping plate 21. By tightening the two nuts, they are made to fit tightly against the side wall of the upper clamping plate 21 to fix the distance between the upper clamping plate 21 and the lower clamping plate 22. Several inclined grooves 222 communicating with the grooves 221 are opened on one side of the lower clamping plate 22. A long groove 223 is also opened on the same side of the lower clamping plate 22, and the long groove 223 is located below the inclined grooves 222.

[0037] The cutting component 3 also includes a blade holder 31. Slide rods 32 are fixedly connected to both ends of the bottom of the blade holder 31. One end of the slide rod 32 is slidably mounted on a fixed plate 13. A matching groove is provided on the fixed plate 13. One end of the slide rod 32 is slidably mounted in the groove. The sliding end of the slide rod 32 is inverted T-shaped to ensure stability during the sliding process. The first cutter 33 is fixedly mounted on one side of the blade holder 31. The second cutter 34 is fixedly connected to the lower side wall of the first cutter 33. The first cutter 33 and the second cutter 34 are set perpendicularly. The cutting end of the first cutter 33 is provided with several arc-shaped blades. The cutting end of the second cutter 34 is also provided with arc-shaped blades.

[0038] The first cutting tool 33 is fixedly connected to the side wall of the connecting plate 35, and the second cutting tool 34 is also fixedly connected to the side wall of the connecting plate 35 to ensure the stability of the second cutting tool 34. The lower end of the connecting plate 35 is snapped with a receiving plate 36, and one end of the receiving plate 36 is inserted into the long groove 223.

[0039] When it is necessary to cut and separate the immature watermelon embryo, the sliding rod 32 brings the cutter closer to the immature watermelon embryo in the clamp 2. At this time, the receiving plate 36 is inserted into the long groove 223. When the first cutter 33 contacts the immature watermelon embryo, it first makes a transverse cut, dividing the embryo into two parts. Since the second cutter 34 is fixedly connected to the lower side wall of the first cutter 33 and the two are set perpendicularly, the second cutter 34 will simultaneously act on the lower end of the transversely cut immature watermelon embryo to make a longitudinal cut, further dividing it into two parts, thereby exposing the embryo. Then, the cutting component 3 is slid back to the initial position along the slide groove, and the receiving plate 36 gradually detaches from the long groove 223. As the blade moves away, since the embryo part has been cut and separated from the main body of the immature watermelon embryo, and under the action of gravity, the exposed embryo will slide smoothly down the inclined groove 222 on the lower clamping plate 22 and finally fall onto the receiving plate 36. Then, the receiving plate 36 can be pulled out from the connecting plate 35 to send the embryo to the cultivation chamber 12. In order to prevent the damaged embryo from being damaged during the fall, the receiving plate 36 is made of rubber.

[0040] The partition 15 divides the culture chamber 12 into several culture areas. The partition 15 is a breathable membrane. Each culture area has an insert plate 16 inserted at the bottom. The culture medium 17 is placed on the insert plate 16, and the embryo is placed on the culture medium 17. This design facilitates the replacement of the culture medium 17. The bottom of the box body 1 has a heating chamber 18 and a heat insulation chamber 19. The heating chamber 18 is located below the insert plate 16 to provide a good temperature culture environment for the embryo. The heat insulation chamber 19 is located below the heating chamber 18 to prevent heat loss.

[0041] A nutrient solution box 14 is fixedly installed at the bottom of the fixed plate 13. The drip irrigation component 4 includes an outlet pipe 41. A connecting pipe 42 is fixedly connected to the center of the bottom of the outlet pipe 41. The connecting pipe 42 is connected to the nutrient solution box 14. Several nozzles 43 are connected to the bottom of the outlet pipe 41. The nozzles 43 are evenly distributed in each culture area. The outlet pipe 41 passes through the partition plate 15.

[0042] It should be noted that a booster pump is fixedly installed inside the nutrient solution box 14 to pressurize the nutrient solution so that it can be sprayed out from the nozzle 43.

[0043] Excess gas (such as carbon dioxide or metabolic waste gas) is generated during the separation and cultivation of immature watermelon embryos. In order to maintain the pressure balance of the cultivation environment, an exhaust component 5 is set up. The exhaust component 5 includes a first exhaust pipe 51 and a second exhaust pipe 52. A first through hole 511 is opened through the first exhaust pipe 51, and a second through hole 521 is opened through the second exhaust pipe 52. The first through hole 511 and the second through hole 521 are connected, and the diameter of the first through hole 511 is smaller than the diameter of the second through hole 521. A float ball 53 is set in the second through hole 521. A filter plate 54 is also fixedly set at the upper end of the second exhaust pipe 52 to prevent dust and other impurities from entering.

[0044] The upper end of the first through hole 511 is a sealing ring. When the gas pressure inside the culture box is within the normal range, the float 53 is located at the lower end of the second through hole 521. At this time, since the diameter of the float 53 is slightly larger than the diameter of the first through hole 511, and the sealing ring at the upper end of the first through hole 511 forms a tight contact with the float 53, it effectively prevents the exchange of external air or gas inside the culture box through the first through hole 511. However, when the gas pressure inside the culture box rises above the set value due to the accumulation of carbon dioxide produced by the respiration of the embryo, the float 53 will be pushed upward by the air pressure. As the float 53 rises, it gradually leaves the sealing ring at the upper end of the first through hole 511, thereby opening the exhaust channel. At this time, the excess gas inside the culture box can be smoothly discharged into the external environment through the through hole, providing a stable and adjustable gas environment for the culture box and ensuring the normal growth and development of the embryo.

[0045] The box body 1 has through holes 111 on both sides of the separation chamber 11. Both sides of the separation chamber 11 are provided with elastic sealing rings 112 that communicate with the holes 111. When the user is separating the parts, he / she can insert his / her hand into the holes 111 on both sides to operate, and the elastic sealing rings 112 fit the wrist.

[0046] In practical use, the culture medium 17 is placed on the insert plate 16, and the insert plate 16 is inserted into the bottom of the culture chamber 12. The user's hand is inserted into the separation chamber 11 through the column hole 111. The immature watermelon embryo is placed in the groove 221 of the upper clamping plate 21 and the lower clamping plate 22 of the clamp 2. The distance between the upper clamping plate 21 and the lower clamping plate 22 is adjusted by rotating the connecting rod 23, and the nut is tightened to ensure that the immature watermelon embryo is firmly clamped. The sliding rod 32 is slid to bring the first cutter 33 and the second cutter 34 closer to the immature watermelon embryo in the clamp 2. When the first cutter 33 contacts the immature watermelon embryo, it makes a transverse cut. At the same time, the second cutter 34 makes a longitudinal cut, separating the immature watermelon embryo and exposing the embryo. After the cut is completed, the cutting component 3 is slid in the opposite direction to make the receiving plate 3... 6. The germ detaches from the long groove 223 and slides down the inclined groove 222 onto the receiving plate 36 under the action of gravity. The receiving plate 36 is pulled out from the connecting plate 35, and the germ is transferred to the culture medium 17 in the culture chamber 12. Nutrient solution is dripped into the culture chamber 12 through the drip irrigation component 4. When the gas pressure in the culture box rises above the set value, the float 53 will be pushed upward by the gas pressure and gradually move away from the sealing ring at the upper end of the first through hole 511. At this time, the excess gas in the culture box can be smoothly discharged into the external environment through the through hole. When the gas pressure in the culture box is within the normal range, the float 53 loses the gas thrust and moves downward to block the first through hole 511 and comes into close contact with the sealing ring, thereby effectively preventing the exchange of external air or gas in the culture box through the first through hole 511.

[0047] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model in any way. Although the present utility model has been disclosed above with reference to preferred embodiments, it is not intended to limit the present utility model. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present utility model. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present utility model without departing from the scope of the present utility model shall still fall within the scope of the present utility model.

Claims

1. A rapid separation and culture box for immature watermelon embryos, comprising a box body (1), wherein a fixing plate (13) is fixedly disposed inside the box body (1), the fixing plate (13) dividing the box body (1) into a separation chamber (11) and a culture chamber (12), characterized in that, The separation chamber (11) is equipped with a clamp (2) for fixing the immature watermelon embryos, and a cutting component (3) is also slidably installed in the separation chamber (11). The culture chamber (12) is equipped with several partitions (15). The top of the culture chamber (12) is equipped with a drip irrigation component (4) for providing nutrient solution to the immature watermelon embryos. The bottom of the culture chamber (12) is equipped with several culture media (17). The top of the separation chamber (11) and the side wall of the culture chamber (12) are both fixedly connected with exhaust components (5). The clamp (2) includes an upper clamp (21) and a lower clamp (22). Both the upper clamp (21) and the lower clamp (22) are provided with a plurality of grooves (221) for placing immature watermelon embryos. The cutting component (3) includes at least a first cutter (33) and a plurality of second cutters (34). The first cutter (33) is used to cut the immature watermelon embryos horizontally, and the second cutters (34) are used to cut the immature watermelon embryos vertically.

2. The rapid separation and culture box for immature watermelon embryos according to claim 1, characterized in that, A connecting rod (23) is provided through the upper clamping plate (21) and the lower clamping plate (22). One end of the connecting rod (23) is rotatably installed in the lower clamping plate (22), and the other end of the connecting rod (23) is threadedly connected to the upper clamping plate (21). Several inclined grooves (222) communicating with the groove (221) are opened on one side of the lower clamping plate (22), and a long groove (223) is also opened on the same side of the lower clamping plate (22).

3. The rapid separation and culture box for immature watermelon embryos according to claim 2, characterized in that, The cutting component (3) also includes a blade holder (31). The blade holder (31) has slide rods (32) fixedly connected to both ends of its bottom. One end of the slide rods (32) is slidably mounted on the fixing plate (13). The first blade (33) is mounted on one side of the blade holder (31). The second blade (34) is fixedly connected to the lower side wall of the first blade (33). The first blade (33) and the second blade (34) are arranged perpendicularly.

4. The rapid separation and culture box for immature watermelon embryos according to claim 3, characterized in that, The first cutting tool (33) is fixedly connected to a connecting plate (35) on its side wall, and the second cutting tool (34) is also fixedly connected to the side wall of the connecting plate (35). A receiving plate (36) is snapped into the lower end of the connecting plate (35), and one end of the receiving plate (36) is inserted into the long groove (223).

5. The rapid separation and culture box for immature watermelon embryos according to claim 1, characterized in that, The partition (15) divides the culture chamber (12) into several culture areas. The partition (15) is a breathable membrane. Each culture area has an insert plate (16) inserted at the bottom. The culture medium (17) is placed on the insert plate (16). The bottom of the box (1) is provided with a heating chamber (18) and a heat insulation chamber (19).

6. The rapid separation and culture box for immature watermelon embryos according to claim 1, characterized in that, The bottom of the fixed plate (13) is fixedly provided with a nutrient solution box (14). The drip irrigation component (4) includes an outlet pipe (41). A connecting pipe (42) is fixedly connected at the center of the bottom of the outlet pipe (41). The connecting pipe (42) is connected to the nutrient solution box (14). Several nozzles (43) are connected to the bottom of the outlet pipe (41).

7. The rapid separation and culture box for immature watermelon embryos according to claim 1, characterized in that, The exhaust component (5) includes a first exhaust pipe (51) and a second exhaust pipe (52). A first through hole (511) is provided in the first exhaust pipe (51), and a second through hole (521) is provided in the second exhaust pipe (52). The diameter of the first through hole (511) is smaller than the diameter of the second through hole (521), and a float ball (53) is provided in the second through hole (521). A filter plate (54) is also fixedly provided at the upper end of the second exhaust pipe (52).

8. The rapid separation and culture box for immature watermelon embryos according to claim 1, characterized in that, The box body (1) has through holes (111) on both sides of the separation chamber (11), and elastic sealing rings (112) communicating with the holes (111) are provided on both sides of the separation chamber (11).

Images