Driving gradient elution device applied to chromatographic column

Abstract

The utility model relates to the field of chromatographic equipment, and discloses a driving gradient elution device applied to a chromatographic column, which comprises a pump parallel system and a gas filtering system used for eliminating bubbles in a mixed solvent, the pump parallel system comprises a multi-way pipe, a mixing pipeline and at least two groups of constant-speed conveying parts, the constant-speed conveying parts are communicated with the multi-way pipe, and the mixing pipeline is communicated with the multi-way pipe. The multi-way pipe comprises at least one output port and at least two input ports, the output end of the multi-way pipe is connected into the gas filtering system through the mixing pipeline, and the gas filtering system is further provided with a liquid outlet, so that the gradient elution device capable of accurately regulating and controlling the proportion of a mixed solvent is provided.

Description

Technical Field

[0001] This utility model relates to the field of chromatography equipment, specifically to a driven gradient elution device applied to a chromatography column. Background Technology

[0002] Chromatography, a separation method based on the interaction between the mobile and stationary phases, has been widely applied in chemical analysis, pharmaceuticals, biology, and environmental monitoring. During chromatography, gradient elution effectively improves separation efficiency and enhances the ability to resolve complex samples. Currently, non-instrumental chromatography typically uses isocratic elution. Traditional non-instrumental gradient elution devices often rely on manual or simple tubing mixing, making precise solvent ratio control difficult. This leads to gradient distortion during mixing of samples with stringent elution conditions due to inaccurate ratio control and the generation of small bubbles, resulting in deviations in separation results and affecting the accuracy and reproducibility of the experiment. Utility Model Content

[0003] The present invention aims to provide a gradient elution device for use in chromatography columns, so as to provide a gradient elution device that can more accurately control the ratio of mixed solvents.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: a driven gradient elution device for a chromatography column, comprising a pump parallel system and a filtration system for eliminating bubbles in a mixed solvent. The pump parallel system comprises a multi-port pipe, a mixing pipe, and at least two sets of uniform speed conveying units, all of which are connected to the multi-port pipe. The multi-port pipe comprises at least one output port and at least two input ports. The output end of the multi-port pipe is connected to the filtration system through the mixing pipe. The filtration system is also provided with a liquid outlet.

[0005] The beneficial effects of this plan are:

[0006] This technical solution includes a parallel pump system with at least two uniform-speed delivery units. Each unit is connected to a multi-port pipe and delivers liquid in parallel. In practical use, different solvents are introduced into the uniform-speed delivery units. Each unit delivers liquid at a set speed, ensuring a continuous and stable mixing ratio of the two solvents and allowing for precise control of the gradient changes in the mixed solvent ratio. A filtration system is also included to eliminate small bubbles generated during solvent mixing. These bubbles can cause uneven solvent mixing, potentially interfering with the flow stability of the liquid during elution and affecting the separation behavior of substances within the chromatography column. The filtration system further ensures the reliability and stability of the chromatographic results, improving the application effect of the chromatography column.

[0007] Preferably, as an improvement, the gas filtration system includes a mixing bottle filled with molecular sieves, and an outlet located at the bottom of the mixing bottle with an outlet pipe connected to the outlet.

[0008] The beneficial effects are as follows: when molecular sieves are filled into the mixing bottle, their porous structure can capture and retain tiny air bubbles in the mixture. Because the tiny air bubbles in the mixture are captured and adsorbed by the pores of the molecular sieves, they eventually aggregate into large air bubbles and remain on the top of the mixing bottle. At the same time, it helps the two solvents to fully mix and contact in the mixing bottle, promotes the full mixing and degassing of the fluid, and ensures that the gradient eluent is both uniform and free of air bubbles.

[0009] Preferably, as an improvement, the number of inlets is the same as the number of uniform speed conveying units, and each inlet is provided with a rotating guide unit that drives the passing fluid to rotate.

[0010] The beneficial effects are as follows: by setting a rotating guide at the inlet, the fluid will rotate and flow as it passes through, breaking the laminar flow state, increasing the contact area between the two solvents, promoting the two fluids to complete the initial premixing before entering the filter mixing bottle, and improving the overall mixing efficiency.

[0011] Preferably, as an improvement, the rotating guide section includes a rotating ring rotatably disposed on the inner wall of the inlet, and the inner wall of the rotating ring is spirally provided with multiple guide plates along the flow direction, the guide plates being planar plates.

[0012] The beneficial effects are as follows: the rotating ring rotates in conjunction with the inlet, and when the fluid flows through, it contacts the guide plate, generating a tangential force that drives the rotating ring to rotate. This rotation of the rotating ring, in turn, causes the fluid to rotate and flow within the multi-pass pipe for initial mixing.

[0013] Preferably, as an improvement, the mixing bottle is an inverted wide-mouth bottle, and the opening of the wide-mouth bottle is provided with a sealing rubber stopper to facilitate the entry of the liquid outlet pipe and the mixing pipe into the mixing bottle.

[0014] The beneficial effect is that the sealing of the wide-mouth bottle is ensured by setting a sealing rubber stopper.

[0015] Preferably, as an improvement, the outlet end of the mixing pipe is higher than the inlet end of the outlet pipe.

[0016] The beneficial effects are as follows: the liquid outlet end of the mixing pipe is higher than the liquid inlet end of the liquid outlet pipe, so that the mixed solvent enters and flows from top to bottom through the molecular sieve bed, causing the microbubbles to rise naturally along the wall, accelerating the separation of bubbles from the liquid phase, and the solvent that eliminates bubbles flows out under the action of gravity.

[0017] Preferably, as an improvement, the constant speed delivery unit is a peristaltic pump.

[0018] The beneficial effects are as follows: the peristaltic pump delivers liquid by squeezing the tubing with rollers, and the flow rate is only related to the pump speed, independent of changes in the viscosity or pressure of the liquid, ensuring uniform liquid delivery and meeting the requirements of gradient elution for precise flow rate control.

[0019] Preferably, as an improvement, the peristaltic pump is an adjustable-speed peristaltic pump.

[0020] Preferably, as an improvement, it also includes a chromatography column, with the other end of the outlet tube connected to the chromatography column and into which a mixed solvent is introduced. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of an embodiment of the present utility model;

[0022] Figure 2 This is a top view schematic diagram of an embodiment of the present utility model;

[0023] Figure 3 for Figure 1 Enlarged view of point A in the middle;

[0024] Figure 4 This is a schematic diagram of the multi-port pipe structure according to an embodiment of the present invention;

[0025] Figure 5 This is a cross-sectional view of the input port in an embodiment of this utility model.

[0026] The reference numerals in the accompanying drawings include: iron stand 1, molecular sieve 2, multi-port pipe 3, mixing pipe 4, uniform speed conveying unit 5, mixing bottle 6, liquid outlet pipe 7, output port 8, input port 9, rotating ring 10, guide plate 11, sealing rubber stopper 12, first solvent pool 13, and second solvent pool 14. Detailed Implementation

[0027] The following detailed description is provided through specific implementation methods and examples:

[0028] The preferred embodiments of this utility model are basically as shown in the appendix. Figure 1-5 As shown, Figure 1 and Figure 2The gradient elution device applied to a chromatography column shown includes a parallel pump system and a filtration system for eliminating air bubbles in the mixed solvent. The parallel pump system includes a multi-port pipe 3, a mixing pipe 4, and at least two sets of uniform speed delivery units 5. There are various mechanisms that can achieve uniform speed fluid delivery, such as screw pumps and gear pumps. To make the structure simple, reliable, and easy to assemble, the preferred embodiment of this invention is as follows: taking the mixing of two solvents as an example, a first solvent pool 13 and a second solvent pool 14 are provided. The uniform speed delivery units 5 are all peristaltic pumps. The peristaltic pumps deliver liquid by squeezing the tubing with rollers. The two peristaltic pumps deliver the solvents in the first solvent pool 13 and the second solvent pool 14 respectively. The flow rate of the peristaltic pump is only related to the pump speed and is independent of the viscosity or pressure changes of the liquid, ensuring uniform speed liquid delivery and meeting the requirements of gradient elution for precise flow rate control. The uniform speed delivery units 5 are all connected to the multi-port pipe 3 and deliver liquid to it. The other end of the multi-port pipe 3 is connected to the filtration system through the mixing pipe 4. The filtration system is also provided with a liquid outlet. To enable this device to be applicable to various working conditions and application scenarios, the preferred embodiment of this utility model is that the peristaltic pump selected in this utility model is an adjustable speed peristaltic pump.

[0029] This technical solution includes a parallel pump system with at least two uniform speed delivery units 5. Each uniform speed delivery unit 5 is connected to a multi-port pipe 3 and delivers liquid to it in parallel. In actual use, different solvents are introduced into the uniform speed delivery units 5. Each uniform speed delivery unit 5 delivers liquid at a set speed to ensure that the two solvents and the mixing ratio remain stable and can accurately control the gradient change of the mixed solvent ratio. At the same time, a gas filtration system is also provided to eliminate small bubbles generated during solvent mixing. Small bubbles can cause uneven solvent mixing, which may interfere with the flow stability of the liquid during elution and affect the separation behavior of substances in the chromatography column. By setting up a gas filtration system, the reliability and stability of the chromatography experimental results can be further guaranteed, and the application effect of the chromatography column can be improved.

[0030] There are various methods for eliminating air bubbles, such as vacuuming or ultrasonic vibration. To ensure a simple, reliable structure and ease of assembly, the preferred embodiment of this invention is as follows: Figure 3 As shown, the filtration system includes a mixing bottle 6 filled with a molecular sieve 2. The outlet is located at the bottom of the mixing bottle 6 and connected to an outlet pipe 7. The molecular sieve 2, filling the mixing bottle 6, has a porous structure that captures and retains microbubbles in the mixture. These microbubbles, captured and adsorbed by the pores of the molecular sieve 2, eventually aggregate into larger bubbles that remain at the top of the mixing bottle 6. This process also facilitates thorough mixing and contact of the two solvents within the mixing bottle 6, promoting complete mixing and degassing of the fluid and ensuring a uniform and bubble-free gradient eluent. To ensure a simple, reliable, and easy-to-assemble structure, the preferred embodiment of this invention further includes a chromatography column. The other end of the outlet pipe 7 is connected to the chromatography column, through which the mixed solvent is introduced.

[0031] To ensure that this equipment can be stably applied to various working conditions, the preferred embodiment of this utility model is as follows: Figure 4 As shown, the multi-port pipe 3 includes at least one output port 8 and at least two input ports 9. The number of input ports 9 is the same as the number of uniform speed conveying units 5. Each input port 9 is equipped with a rotating guide that drives the passing fluid to rotate. By setting the rotating guide, the fluid rotates as it passes through, breaking the laminar flow state, increasing the contact area between the two solvents, and promoting the two fluids to complete preliminary premixing before entering the filter mixing bottle 6, thereby improving the overall mixing efficiency. There are various ways to implement the rotating guide, such as using a series of rotating bodies that can generate tangential force with the fluid, such as a spiral ring. To make the structure simple, reliable, and easy to assemble, the preferred embodiment adopted by this utility model is as follows: Figure 5 As shown, the rotating guide section includes a rotating ring 10 rotatably disposed on the inner wall of the inlet 9. Multiple guide plates 11 are spirally arranged on the inner wall of the rotating ring 10 along the flow direction. Specifically, the length direction of the guide plates 11 is not parallel to the fluid flow direction. To enable better flow guidance, the preferred embodiment of this invention is that the guide plates 11 are planar plates, and the rotating ring 10 is rotatably engaged with the inlet 9. The rotatable engagement form is not limited; for example, the rotating ring 10 can be embedded in the inner wall of the inlet 9, or the inner wall of the inlet 9 can be provided with limiting rings at both ends, with the rotating ring 10 disposed between the limiting rings. Through the above arrangement, when the fluid flows through, it contacts the guide plates 11, generating a tangential force that drives the rotating ring 10 to rotate, thereby causing the rotating ring 10 to rotate and the fluid to rotate and flow within the multi-pass pipe 3 for initial mixing.

[0032] To ensure a simple, reliable, and easy-to-assemble structure, the preferred embodiment of this invention uses an inverted wide-mouth bottle 6. The opening of the wide-mouth bottle is equipped with a sealing rubber stopper 12 to facilitate the entry of the liquid outlet pipe 7 and the mixing pipe 4 into the mixing bottle 6. The sealing rubber stopper 12 ensures the airtightness of the wide-mouth bottle. The invention also includes an iron stand 1, which supports the mixing bottle 6. To further ensure a simple, reliable, and easy-to-assemble structure, the preferred embodiment of this invention uses a liquid outlet pipe 4 located at the top of the mixing bottle 6 and higher than the inlet end of the liquid outlet pipe 7. This allows the mixed solvent to flow downwards through the molecular sieve 2 bed, causing microbubbles to rise naturally along the wall, accelerating the separation of bubbles from the liquid phase. The solvent that eliminates the bubbles flows out under gravity.

[0033] The above descriptions are merely embodiments of this utility model. Commonly known technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solution of this utility model. These modifications and improvements should also be considered within the scope of protection of this utility model, and will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A driven gradient elution device for use in chromatography columns, characterized in that: The system includes a pump parallel system and a filtration system for eliminating air bubbles in the mixed solvent. The pump parallel system includes a multi-port pipe (3), a mixing pipe (4), and at least two sets of uniform speed conveying units (5). The uniform speed conveying units (5) are all connected to the multi-port pipe (3). The multi-port pipe (3) includes at least one output port (8) and at least two input ports (9). The output end of the multi-port pipe (3) is connected to the filtration system through the mixing pipe (4). The filtration system is also provided with a liquid outlet.

2. The driven gradient elution device for a chromatography column according to claim 1, characterized in that: The gas filtration system includes a mixing bottle (6), which is filled with a molecular sieve (2). The liquid outlet is located at the bottom of the mixing bottle (6) and is connected to a liquid outlet pipe (7).

3. The driven gradient elution device for a chromatography column according to claim 1, characterized in that: The number of inlet ports (9) is the same as the number of uniform speed conveying units (5), and each inlet port (9) is provided with a rotating guide unit that drives the fluid to rotate.

4. The driven gradient elution device for a chromatography column according to claim 3, characterized in that: The rotating guide section includes a rotating ring (10) that is rotatably disposed on the inner wall of the inlet (9). Multiple guide plates (11) are spirally disposed on the inner wall of the rotating ring (10) along the flow direction. The guide plates (11) are flat plates.

5. The driven gradient elution device for a chromatography column according to claim 2, characterized in that: The mixing bottle (6) is an inverted wide-mouth bottle, and the opening of the wide-mouth bottle is provided with a sealing rubber stopper (12) to facilitate the entry of the liquid outlet pipe (7) and the mixing pipe (4) into the mixing bottle (6).

6. The driven gradient elution device for a chromatography column according to claim 2, characterized in that: The outlet end of the mixing pipe (4).

7. The driven gradient elution device for a chromatography column according to claim 1, characterized in that: The constant speed conveying unit (5) is a peristaltic pump.

8. The driven gradient elution device for a chromatography column according to claim 7, characterized in that: The peristaltic pump is an adjustable-speed peristaltic pump.

9. The driven gradient elution device for a chromatography column according to any one of claims 1 to 8, characterized in that: It also includes a chromatography column, with the other end of the outlet tube (7) connected to the chromatography column and a mixed solvent introduced into it.

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