96-well microwell plate sealing and puncturing film structure
By designing a puncture-friendly sealing membrane structure, the problem of complex operation of the sealing membrane for 96-well microplates was solved, achieving convenient puncture and maintaining sealing effect, and preventing reagent tube contamination.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- AISAI BIOTECHNOLOGY (SHENZHEN) CO LTD
- Filing Date
- 2025-04-25
- Publication Date
- 2026-04-14
AI Technical Summary
The existing 96-well microplate sealing membrane is complicated to operate and difficult to puncture efficiently and conveniently, which affects the sealing effect and operation efficiency of the reagent tube.
Design a sealing film structure comprising a protective film, a film substrate, a double-sided adhesive layer, and a release film. The film substrate has an easy-pierce structure, the double-sided adhesive layer has through holes, and the protective film is adhesive. During operation, first tear off the release film, attach the easy-pierce structure, and then tear off the protective film, simplifying the piercing process.
It enables convenient puncture of the sealing membrane, maintains the sealing effect, reduces operational complexity, and protects the membrane's adhesiveness to prevent reagent tube contamination.
Smart Images

Figure CN224122603U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of 96-hole microplate sealing and puncture technology, specifically a 96-hole microplate sealing and puncture membrane structure. Background Technology
[0002] In various detection experiments using 96-well microplate reagent encapsulation, the reagent is usually added to the tube of the 96-well microplate first, and then the 96-well microplate is placed in the test apparatus for reaction or temporary storage. In order to prevent sample loss and cross-contamination caused by evaporation or leakage of the test reagent, a thin film is sealed on the 96-well microplate to encapsulate the reagent in its respective tube.
[0003] When applying the sealing film, the 96-well microplate is usually completely sealed with a thin film. When it is necessary to take or add liquid into the reagent tube, the sealing film needs to be pierced with a needle, which is complicated, labor-intensive and inconvenient. Utility Model Content
[0004] The purpose of this invention is to provide a 96-well microporous plate sealing and puncture membrane structure to solve the problems mentioned in the background art.
[0005] To achieve the above objectives, this utility model provides the following technical solution: a 96-hole microporous plate sealing and puncture membrane structure, comprising a sealing membrane body, wherein the sealing membrane body comprises a protective film, a thin film substrate, a double-sided adhesive layer, and a release film arranged in sequence; the protective film is used to protect the entire sealing membrane body, the inner surface of the protective film is adhesive, the inner surface of the protective film is adhered to the first surface of the thin film substrate, the thin film substrate has an easy-puncture structure, the second surface of the thin film substrate is adhered to the double-sided adhesive layer, the double-sided adhesive layer is provided with through holes, the through holes are arranged opposite to the easy-puncture structure, and the release film is adhered to the double-sided adhesive layer.
[0006] Preferably, the puncture-resistant structure is a star-shaped insertion port provided on the film substrate. The star-shaped insertion port includes multiple contact pieces, which are not connected but abut against each other.
[0007] Furthermore, there are multiple puncture-resistant structures, and the number of through holes on the double-sided adhesive layer is the same as the number of puncture-resistant structures.
[0008] Furthermore, the protective film has a first protrusion that is not directly opposite the film substrate, and the release film has a second protrusion that is not directly opposite the double-sided adhesive layer.
[0009] Furthermore, the first protrusion and the second protrusion are arranged facing each other.
[0010] Compared with existing technologies, the 96-well microplate sealing and puncture membrane structure provided in this application allows for easy application. First, the release film is peeled off, allowing the double-sided adhesive layer to be applied to the testing fixture. The puncture-easy structure is then aligned with the PCR reagent tube. Next, the protective film is peeled off. Due to the puncture-easy structure, the sealing film can be easily penetrated without affecting its sealing effect. The operation is simple and convenient. Furthermore, because the protective film is adhesive, it can be further adhered to a film substrate, preventing contamination inside the reagent tube. Attached Figure Description
[0011] Figure 1 This is a schematic diagram of the structure of this utility model;
[0012] Figure 2 This is an exploded structural diagram of the present invention. Detailed Implementation
[0013] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, not all embodiments. In addition, the terms "first", "second", "third", "upper", "lower", "left", "right", etc. are used for descriptive purposes only and should not be construed as indicating or implying relative importance. At the same time, in the description of the present utility model, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present utility model.
[0014] Reference Figure 1-2 As shown, this utility model provides a 96-hole microporous plate sealing and puncture membrane structure, including a sealing membrane body. The sealing membrane body includes a protective film 1, a thin film substrate 2, a double-sided adhesive layer 3, and a release film 4 arranged in sequence. The protective film 1 is used to protect the entire sealing membrane body. The inner surface of the protective film 1 is adhesive. The inner surface of the protective film 1 is adhered to the first surface of the thin film substrate 2. The thin film substrate 2 has an easy-puncture structure 21. The second surface of the thin film substrate 2 is adhered to the double-sided adhesive layer 3. The double-sided adhesive layer 3 is provided with through holes. The through holes are arranged opposite to the easy-puncture structure 21. The release film 4 is adhered to the double-sided adhesive layer 3.
[0015] This application provides a 96-well microplate sealing and puncture membrane structure. In use, the release film 4 is first torn off, allowing the double-sided adhesive layer 3 to be attached to the testing fixture, so that the easy-puncture structure 21 is aligned with the PCR reagent tube. Then, the protective film 1 is torn off. Due to the design of the easy-puncture structure 21, it is extremely easy to pass through the sealing film without affecting the sealing effect of the sealing film. The operation is simple and very convenient. Since the protective film 1 is adhesive, it can also be attached to the film substrate 2 to prevent contamination inside the reagent tube.
[0016] Preferably, the puncture-resistant structure 21 is a star-shaped insertion port provided on the thin film substrate 2. The star-shaped insertion port includes multiple contact pieces that are not connected but abut against each other. In this way, the multiple contact pieces are not connected but in contact, which facilitates puncture of the thin film substrate 2 while ensuring its sealing effect.
[0017] Furthermore, there are multiple puncture-resistant structures 21, and the number of through holes on the double-sided adhesive layer 3 is the same as the number of puncture-resistant structures 21.
[0018] Furthermore, the protective film 1 has a first protrusion, which is not directly opposite to the film substrate 2, and the release film 4 has a second protrusion, which is not directly opposite to the double-sided adhesive layer 3.
[0019] Furthermore, the first protrusion and the second protrusion are arranged facing each other.
[0020] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.
Claims
1. A 96-well microplate sealing and puncture membrane structure, comprising a sealing membrane body, characterized in that: The sealing membrane includes a protective film, a film substrate, a double-sided adhesive layer, and a release film arranged in sequence. The protective film is used to protect the entire sealing membrane. The inner surface of the protective film is adhesive. The inner surface of the protective film is adhered to the first surface of the film substrate. The film substrate has an easy-to-puncture structure. The double-sided adhesive layer is adhered to the second surface of the film substrate. The double-sided adhesive layer is provided with through holes. The through holes are arranged opposite to the easy-to-puncture structure. The release film is adhered to the double-sided adhesive layer.
2. The 96-well microporous plate sealing and puncture membrane structure according to claim 1, characterized in that: The easily puncturable structure is a star-shaped insertion port provided on the film substrate. The star-shaped insertion port includes multiple contact pieces, which are not connected but abut against each other.
3. The 96-well microporous plate sealing and puncture membrane structure according to claim 2, characterized in that: There are multiple puncture-resistant structures, and there are multiple through holes on the double-sided adhesive layer, which are the same number as the number of puncture-resistant structures.
4. The 96-well microporous plate sealing and puncture membrane structure according to claim 3, characterized in that: The protective film has a first protrusion that is not directly opposite the film substrate, and the release film has a second protrusion that is not directly opposite the double-sided adhesive layer.
5. The 96-well microporous plate sealing and puncture membrane structure according to claim 4, characterized in that: The first protrusion and the second protrusion are arranged facing each other.