Chromatographic column device
By designing the distributor and sieve plate, the problem of uneven fluid distribution is solved, enabling larger-scale and more efficient fluid distribution, reducing the risk of cross-contamination, improving separation and production efficiency, and reducing the difficulty and cost of wastewater treatment.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- HISUN PHARMACEUTICAL (HANGZHOU) CO LTD
- Filing Date
- 2025-04-25
- Publication Date
- 2026-04-17
AI Technical Summary
Existing ion exchange column designs result in uneven fluid distribution, increasing equipment investment and operating costs, affecting separation efficiency, increasing the difficulty of wastewater treatment, and posing a risk of cross-contamination.
The system employs a distributor and sieve plate structure. The distributor has large-diameter distribution holes, while the sieve plate has small-diameter sieve holes, which enables uniform distribution of fluid and reduces the risk of cross-contamination.
It improves the uniformity of fluid distribution, reduces the risk of cross-contamination, increases separation and production efficiency, reduces wastewater volume, and lowers equipment investment and maintenance costs.
Smart Images
Figure CN224126620U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of chromatography technology, and in particular to a chromatography column apparatus. Background Technology
[0002] In fermentation broth extraction production lines, the design of ion exchange columns is crucial, directly impacting separation efficiency, production capacity, and cost. Currently, the upper and lower end caps of the column typically use a perforated plate with a filter cap. However, this design has several drawbacks during industrial scale-up: The perforated plate and filter cap may lead to uneven fluid distribution across the column bed. To ensure synchronization across the entire horizontal plane during loading, washing, and elution, a liquid layer of at least 1 meter above the column bed is required. This design limits the column diameter, increases the required plant area, and significantly increases equipment investment and operating costs. After production begins, more manpower is needed for operation and maintenance, increasing workload and related expenses. The presence of a liquid layer leads to cross-contamination between the loading solution and the eluent, causing sample dilution at the column tip, affecting separation efficiency, and resulting in product tailing and insufficient purity. Low separation efficiency results in large wastewater volumes, increasing the difficulty of wastewater treatment and the environmental burden.
[0003] In summary, how to effectively address the shortcomings in the design of ion exchange columns in current fermentation broth extraction production lines is a problem that urgently needs to be solved by those skilled in the art. Utility Model Content
[0004] The purpose of this invention is to provide a chromatography column device that achieves larger-scale, more efficient, and more uniform fluid distribution, which helps to improve the performance and production efficiency of ion exchange columns, while reducing the risk of cross-contamination.
[0005] To solve the above-mentioned technical problems, this utility model provides the following technical solution:
[0006] A chromatography column apparatus includes a column tube, an upper cover assembly and a lower cover assembly respectively installed at the upper and lower ends of the column tube. The upper cover assembly and the lower cover assembly include a cover plate, a distributor, and a sieve plate. The cover plate, the distributor, and the sieve plate are distributed sequentially from the outside to the inside. The upper cover plate has a mobile phase material inlet, and the lower cover plate has a mobile phase material outlet. The distributor includes a feed port communicating with the mobile phase material inlet or the mobile phase material outlet, multiple flow channels formed on the plate surface and communicating with the feed port, and multiple distribution holes provided on the flow channels. The flow channels of the upper distributor face the upper cover plate, and the flow channels of the lower distributor face the lower sieve plate. The sieve plate includes multiple sieve holes communicating with the distribution holes, and the diameter of the distribution holes is larger than the diameter of the sieve holes.
[0007] Optionally, the sieve plate has multiple screening areas with intervals between adjacent screening areas, and multiple sieve holes are distributed in each screening area. The distributor has multiple distribution areas, and the distribution areas are the same as the vertical projection areas of the screening areas. The number of distribution holes contained in the distribution areas is proportional to the area of the distribution areas.
[0008] Optionally, a dividing rib is provided between the sieve plate and the distributor, one end of the dividing rib abutting against the surface of the distributor and the other end abutting against the surface of the sieve plate, the sieve plate being parallel to the distributor.
[0009] Optionally, the sieve plate and the dividing rib are integrally formed, and the screening area is divided into independent areas by the dividing rib.
[0010] Optionally, the mating surface between the dispenser and the sieve plate is a plane, and the cover plate is fitted to the mating surface of the dispenser and connected to the column tube by fasteners.
[0011] Optionally, the dispenser has a groove on the surface facing the sieve plate, the plane is disposed on the bottom surface of the groove, and the sieve plate is embedded in the groove.
[0012] Optionally, the depth of the groove is equal to the thickness of the sieve plate.
[0013] Optionally, the groove is clearance-fitted with the sieve plate, and the sidewall of the groove or the sidewall of the sieve plate has a sealing groove, in which a sealing ring is provided.
[0014] Optionally, the mounting portion of the sieve plate has a through hole, and the bottom surface of the groove is provided with a threaded hole that matches the through hole. The sieve plate and the distributor are connected by a connector.
[0015] Optionally, the mating surfaces of the sieve plate and the distributor are provided with interlocking concave and convex surfaces.
[0016] The chromatography column apparatus provided by this utility model has an upper cover assembly installed at the upper end of the column tube and a lower cover assembly installed at the lower end of the column tube. The upper cover assembly and the lower cover assembly have similar structures, both including a cover plate, a distributor and a sieve plate, and the cover plate, distributor and sieve plate are distributed from the outside to the inside.
[0017] The distributor includes a feed inlet, flow channels, and distribution holes. The feed inlet is connected to either the inlet or outlet of the mobile phase material. Multiple flow channels are formed on the distributor plate; the flow channels of the upper distributor face the upper cover plate, and the flow channels of the lower distributor face the lower screen plate. The inlet of each flow channel is connected to the feed inlet, and the material flows along the flow channel. Each flow channel has several branches, and each branch ends in a distribution hole, thus distributing the material passing through the distributor.
[0018] The sieve plate is located inside the distributor and is covered with sieve holes. These sieve holes are connected to the distribution holes on the distributor, and the diameter of the sieve holes is smaller than that of the distribution holes.
[0019] When the mobile phase enters the distributor from the feed inlet, it flows along the flow channel and enters the screen holes evenly through the distribution holes, and then passes through the column bed. Before the mobile phase flows out from the mobile phase material outlet, the material first enters the flow channel through the screen plate, flows along the flow channel to the feed inlet, and flows out of the column bed from the mobile phase material outlet.
[0020] A liquid layer is laid above the column bed to keep it moist and prevent the stationary phase from drying out and affecting the separation effect. Because the distributor and sieve plate are used in a stacked manner, only a 20cm-50cm liquid layer needs to be maintained between the column bed and the top cover plate after column loading. This allows space for volume changes of the ion exchange resin in acid and alkali conditions, while further improving the synchronicity of column loading, washing, and elution across the entire horizontal plane, and enhancing internal adjustability.
[0021] The chromatography column apparatus provided by this invention features a distributor with distribution holes that work in conjunction with sieve holes on a sieve plate to distribute the material at the inlet and outlet, working together to distribute and collect the mobile phase. The larger diameter of the distribution holes allows for the containment of a certain amount of mobile phase, guiding it to the sieve plate and acting as a flow divider to ensure uniform distribution of the fluid across the entire column bed, reducing efficiency losses caused by uneven fluid distribution. Conversely, the smaller diameter of the sieve holes allows for uniform dispersion of the mobile phase across the entire column bed cross-section, ensuring sufficient contact between the mobile and stationary phases, thereby achieving effective separation of sample components. Attached Figure Description
[0022] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0023] Figure 1 A schematic diagram of the structure of a chromatography column apparatus provided in a specific embodiment of this utility model;
[0024] Figure 2 This is a front view of the chromatography column apparatus;
[0025] Figure 3 for Figure 2 Enlarged view of point A in the middle;
[0026] Figure 4 This is a schematic diagram of the distributor's structure;
[0027] Figure 5 This is a schematic diagram of the sieve plate structure.
[0028] Figure label:
[0029] 1-Column tube; 2-Upper cover assembly; 3-Lower cover assembly; 4-Cover plate; 5-Distributor; 6-Sieve plate; 7-Resin filler outlet; 8-Resin filler inlet; 9-Mobile phase material inlet; 10-Mobile phase material outlet; 11-Observation window; 51-Flow channel; 52-Distribution hole; 53-Material outlet; 61-Sieve hole. Detailed Implementation
[0030] The core of this invention is to provide a chromatography column device that achieves larger-scale, more efficient, and more uniform fluid distribution, which helps to improve the performance and production efficiency of ion exchange columns, while reducing the risk of cross-contamination.
[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0032] Please refer to Figures 1 to 5 , Figure 1 A schematic diagram of the structure of a chromatography column apparatus provided in a specific embodiment of this utility model; Figure 2 This is a front view of the chromatography column apparatus; Figure 3 for Figure 2 Enlarged view of point A in the middle; Figure 4 This is a schematic diagram of the distributor's structure; Figure 5 This is a schematic diagram of the sieve plate structure.
[0033] In one specific embodiment, the chromatography column device provided by this utility model includes a column tube 1, an upper cover assembly 2 and a lower cover assembly 3 respectively installed at the upper and lower ends of the column tube 1. The upper cover assembly 2 and the lower cover assembly 3 include a cover plate 4, a distributor 5 and a sieve plate 6. The cover plate 4, the distributor 5 and the sieve plate 6 are distributed sequentially from the outside to the inside. The upper cover plate is provided with a mobile phase material inlet 9 and the lower cover plate is provided with a mobile phase material outlet 10. The distributor 5 includes a material inlet 53 communicating with the mobile phase material inlet 9 or the mobile phase material outlet 10, multiple flow channels 51 opened on the plate surface and communicating with the material inlet 53, and multiple distribution holes 52 provided on the flow channels 51. The flow channels 51 of the upper distributor face the upper cover plate, and the flow channels 51 of the lower distributor face the lower sieve plate. The sieve plate 6 includes multiple sieve holes 61 communicating with the distribution holes 52. The diameter of the distribution holes 52 is larger than the diameter of the sieve holes 61.
[0034] In the above structure, the chromatography column apparatus includes a column tube 1, an upper cover assembly 2, and a lower cover assembly 3. The column tube 1, as the main body of the chromatography column, is usually a vertically placed cylindrical tube. The diameter of the column is increased when the sample volume is large. The inside of the column tube 1 is filled with a stationary phase to achieve the separation of the components in the sample.
[0035] The upper cover assembly 2 is installed at the upper end of the column tube 1, and the lower cover assembly 3 is installed at the lower end of the column tube 1. The upper cover assembly 2 and the lower cover assembly 3 have similar structures, both including a cover plate 4, a distributor 5, and a sieve plate 6, and the cover plate 4, distributor 5, and sieve plate 6 are distributed from the outside to the inside. Specifically, the upper cover assembly 2 consists of the following components from top to bottom: upper cover plate, upper distributor, and upper sieve plate; the lower cover assembly 3 consists of the following components from top to bottom: lower sieve plate, lower distributor, and lower cover plate.
[0036] The cover plate 4 is the outermost component of the upper cover assembly 2 and the lower cover assembly 3, serving to seal and support. The upper cover plate is provided with a mobile phase material inlet 9, through which materials such as the mobile phase and sample solution enter the column tube 1; the lower cover plate is provided with a mobile phase material outlet 10, through which materials such as the mobile phase and separated components after passing through the column bed are discharged.
[0037] The distributor 5 includes a feed inlet 53, flow channels 51, and distribution holes 52. The feed inlet 53 is connected to the mobile phase material inlet 9 or the mobile phase material outlet 10. Multiple flow channels 51 are formed on the surface of the distributor 5. The flow channels 51 of the upper distributor face the upper cover plate, and the flow channels 51 of the lower distributor face the lower sieve plate. The inlet of each flow channel 51 is connected to the feed inlet 53, and the material flows along the flow channels 51. Each flow channel 51 has several branches, and each branch end has a distribution hole 52, thereby achieving a dispersed arrangement of the distribution holes 52 to disperse the material passing through the distributor 5. Preferably, the distributor 5 is an H-TREE distributor 5, which is a fluid distribution device used in a liquid chromatography system.
[0038] The sieve plate 6 is located inside the distributor 5 and is covered with sieve holes 61, which communicate with the distribution holes 52 on the distributor 5. The diameter of the sieve holes 61 is smaller than that of the distribution holes 52, and the diameter of the sieve holes 61 is very small, for example, 50µm.
[0039] When the mobile phase enters the distributor 5 from the feed inlet 53, it flows along the flow channel 51 and enters the sieve hole 61 evenly through the distribution hole 52, and then passes through the column bed. During this process, the distributor 5 ensures that the mobile phase is evenly distributed on the cross-section of the column bed, avoiding problems such as the mobile phase directly impacting the column bed or excessively high local flow velocity. The sieve plate 6 can further improve the separation effect by making the mobile phase more evenly dispersed when entering the column bed.
[0040] Before the mobile phase flows out of the mobile phase material outlet 10, the material first enters the flow channel 51 through the screen plate 6, flows along the flow channel 51 to the feed port 53, and flows out of the column bed from the mobile phase material outlet 10. In this process, the screen plate 6 can make the mobile phase more evenly collected when entering the column bed, and the distributor 5 not only supports the screen plate 6, but also makes the mobile phase flow out evenly.
[0041] A liquid layer is laid above the column bed to keep it moist and prevent the stationary phase from drying out and affecting the separation effect. Because the distributor 5 and sieve plate 6 are used in a stacked manner, only a 20cm-50cm liquid layer needs to be maintained between the column bed and the top cover plate after column loading. For example, 20cm allows space for volume changes of the ion exchange resin in acid and alkali conditions, while also further improving the synchronicity of column loading, washing, and elution across the entire horizontal plane and enhancing internal adjustability.
[0042] The chromatography column apparatus provided by this invention allows the mobile phase to enter the column bed uniformly through the feed inlet 53 of the upper distributor during chromatography, passing sequentially through the flow channel 51, distribution hole 52, and sieve hole 61. The separated mobile phase and components then enter the distribution hole 52, flow channel 51, and feed inlet 53 of the lower distributor through the sieve hole 61 of the lower sieve plate, and finally exit from the mobile phase material outlet 10 of the lower cover plate. During this process, the distribution hole 52 on the distributor 5 and the sieve hole 61 on the sieve plate 6 work together to distribute the material at the inlet and outlet, working together to distribute and collect the mobile phase. The larger diameter of the distribution hole 52 allows it to hold a certain amount of mobile phase and guide it to the sieve plate 6, acting as a diverter to ensure uniform distribution of the fluid throughout the column bed, reducing efficiency losses caused by uneven fluid distribution. The smaller diameter of the sieve hole 61 allows the mobile phase to be evenly dispersed across the entire column bed cross-section, ensuring sufficient contact between the mobile phase and the stationary phase, thereby achieving effective separation of sample components.
[0043] Based on the above specific embodiments, the sieve plate 6 has multiple screening areas with intervals between adjacent screening areas, and multiple sieve holes 61 are distributed in each screening area. The distributor 5 has multiple distribution areas, and the vertical projection area of the distribution area is the same as that of the screening area. The number of distribution holes 52 contained in the distribution area is proportional to the area of the distribution area.
[0044] In one specific embodiment, the sieve plate 6 is divided into multiple screening zones, which may be regularly arranged, such as circular or square areas. There is a certain interval between adjacent screening zones to avoid mutual interference between different screening zones and to ensure that the mobile phase can be distributed and flow independently in each screening zone.
[0045] Multiple sieve holes 61 are evenly distributed within each screening area. The sieve holes 61 have a small aperture and can be of uniform size to ensure a uniform velocity and flow rate distribution of the mobile phase as it passes through the sieve holes 61. The density of the sieve holes 61 can be adjusted according to actual needs to meet different separation precision and flow rate requirements.
[0046] The distributor 5 is divided into multiple distribution zones corresponding to the screening area of the sieve plate 6. The shape and position of the distribution zones match the vertical projection area of the screening area, that is, the vertical projection of each distribution zone can accurately cover the corresponding screening area, so as to ensure that the mobile phase can enter the corresponding screening area uniformly from the distribution zone.
[0047] Within each distribution area, multiple distribution holes 52 are distributed. The number of distribution holes 52 is directly proportional to the area of the distribution area; that is, a larger distribution area contains more distribution holes 52, and vice versa. This ensures that the mobile phase is evenly distributed into each sieve hole 61 within distribution areas of different sizes, avoiding the problem of uneven mobile phase distribution caused by different numbers of distribution holes 52 in different areas.
[0048] During chromatography, after the mobile phase enters the feed inlet 53 of the distributor 5, it first flows through the distribution holes 52 in the distribution area. Since the number and distribution of the distribution holes 52 match the area of the distribution area, the mobile phase can flow uniformly from the distribution holes 52 and enter the corresponding sieving area. In the sieving area, the mobile phase is evenly distributed into the column bed through multiple sieve holes 61, ensuring uniform distribution of the mobile phase upon entering the column bed and improving separation efficiency and effect. The spacing between adjacent sieving areas prevents short-circuiting or mixing of the mobile phase between different sieving areas, ensuring the stability and reliability of the separation process.
[0049] Based on the above specific embodiments, a dividing rib is provided between the sieve plate 6 and the distributor 5. One end of the dividing rib abuts against the surface of the distributor 5, and the other end abuts against the surface of the sieve plate 6. The sieve plate 6 is parallel to the distributor 5.
[0050] In one specific embodiment, the dividing rib can be rectangular or trapezoidal, with one end tightly abutting against the surface of the distributor 5 and the other end tightly abutting against the surface of the sieve plate 6, thereby dividing and supporting the sieve plate 6 and the distributor 5.
[0051] The height of the dividing ribs is equal, ensuring that the distributor 5 is parallel to the sieve plate 6 and that the spacing between the distributor 5 and the sieve plate 6 is uniform. This ensures that the mobile phase can pass through each sieve hole 61 evenly into the column bed during the distribution process, avoiding uneven distribution of the mobile phase caused by uneven spacing, and improving the stability and repeatability of the separation effect.
[0052] Based on the above specific embodiments, the sieve plate 6 and the dividing ribs are integrally formed, and the screening area is divided into independent areas by the dividing ribs.
[0053] In one specific embodiment, the sieve plate 6 and the dividing ribs are integrally formed, and the dividing ribs enhance the strength and structural stability of the sieve plate 6. During the chromatography process, the flow of the mobile phase will generate certain pressure and impact forces, and the dividing ribs can effectively disperse these forces, reduce the deformation of the sieve plate 6 and the distributor 5, and extend the service life of the device.
[0054] The sieve plate 6 can be an integral structure or a split structure, such as being composed of 4 distributed small sieve plates spliced together. Multiple small sieve plates can be connected by welding or mated to the surface of the distributor 5.
[0055] The dividing ribs are arranged according to certain rules between the sieve plate 6 and the distributor 5, and their layout corresponds to the screening area on the sieve plate 6 and the distribution area on the distributor 5. The dividing ribs can be straight, curved, or broken, etc., and are designed according to actual needs to achieve effective control of the distribution of the mobile phase.
[0056] The dividing ribs separate the space between the sieve plate 6 and the distributor 5 into multiple independent regions, each corresponding to a screening area and a distribution area. This prevents the mobile phase from flowing laterally and mixing between different screening areas, ensuring that the mobile phase can only follow a predetermined path, thus enhancing the uniformity and stability of the mobile phase distribution and further improving the separation efficiency.
[0057] Based on the above specific embodiments, the mating surfaces of the distributor 5 and the sieve plate 6 are flat, ensuring a tight fit between the two and thus guaranteeing a uniform pressure distribution within the column. The cover plate 4 is mated to the distributor 5 and connected to the column tube 1 by fasteners, allowing for detachable connection and easy disassembly. This ensures a tight fit between the sieve plate 6, distributor 5, and cover plate 4, reducing gaps between the distributor 5 and the sieve plate 6, and between the distributor 5 and the cover plate 4, improving the sealing of the chromatography column, preventing mobile phase leakage, and enhancing the stability of the device to ensure the smooth progress of the chromatography process.
[0058] Based on the above specific embodiments, the dispenser 5 has a groove on the surface facing the sieve plate 6, with a plane on the bottom surface of the groove, and the sieve plate 6 is embedded in the groove.
[0059] In one specific embodiment, the distributor 5 has a groove on the side facing the sieve plate 6. The shape and size of the groove match the size of the sieve plate 6 to accommodate it. A flat surface is located on the bottom surface of the groove, serving as the contact surface between the distributor 5 and the sieve plate 6, ensuring that the sieve plate 6 can be placed flat on the distributor 5 and providing stable support. The sieve plate 6 is embedded within the groove of the distributor 5, increasing the contact area between them and resulting in a tighter connection, which helps improve the sealing and stability of the entire device.
[0060] Based on the above specific embodiments, the depth of the groove is equal to the thickness of the sieve plate 6. After the sieve plate 6 is embedded, the sieve plate 6 and the distributor 5 are tightly fitted together, and the surface of the sieve plate 6 is flush with the surface of the distributor 5. The groove limits the outer periphery of the sieve plate 6, ensuring the stability of the sieve plate 6 in the groove. This ensures that the stationary phase will not be disturbed by the displacement of the sieve plate 6 during the chromatography process, thereby improving the stability of the chromatography column.
[0061] Based on the above specific embodiments, the groove and the sieve plate 6 are fitted with a clearance, and the side wall of the groove or the side wall of the sieve plate 6 has a sealing groove, and a sealing ring is provided in the sealing groove.
[0062] Based on the above specific embodiments, the side wall of the groove or the side wall of the sieve plate 6 is provided with a sealing groove, and a sealing ring is installed in the sealing groove to further enhance the sealing performance and prevent the mobile phase from leaking from the gap between the sieve plate 6 and the distributor 5.
[0063] Based on the above specific embodiments, the mounting part of the sieve plate 6 has a through hole, and the bottom surface of the groove is provided with a threaded hole that matches the through hole. The sieve plate 6 and the distributor 5 are connected by a connector.
[0064] Based on the above specific embodiments, during installation, the sieve plate 6 is placed in the groove of the distributor 5, and bolts or other connecting parts are passed through the through holes of the sieve plate 6 and screwed into the threaded holes of the distributor 5 to achieve a tight connection between the sieve plate 6 and the distributor 5. Then, the sieve plate 6 and the distributor 5, together with the cover plate 4, are fixed to the column tube 1 with fasteners to ensure the stability and sealing of the entire structure.
[0065] The cover plate 4, distributor 5, and screen plate 6 are detachably connected to the column tube 1, facilitating the inspection, cleaning, and replacement of the screen plate 6 and distributor 5. Specifically, flanges are provided on the edges of the cover plate 4, the distributor 5, and the column tube 1. The flanges have through holes, through which bolts pass and are tightened with nuts, making the connection convenient and easy to disassemble and maintain.
[0066] Based on the above specific embodiments, the mating surfaces of the sieve plate 6 and the distributor 5 are provided with interlocking concave and convex surfaces. These surfaces make the mating surfaces of the sieve plate 6 and the distributor 5 tighter, enhance the sealing performance, and prevent leakage of the flowing phase. At the same time, the interlocking of the concave and convex surfaces can also play a positioning role, ensuring that the sieve plate 6 will not shift during use and improving the stability of the device.
[0067] Based on the above specific embodiments, the side wall of the column tube 1 is provided with an observation window 11, and the height of the observation window 11 is the same as the height of the observation part.
[0068] In one specific embodiment, the observation window 11 is fixedly connected to the outer wall of one side of the column tube 1. By setting the observation window 11, it is convenient for the observer to observe the liquid level, and the observation is accurate during normal operation.
[0069] The pressure relief port and the observation window 11 are located on different surfaces. Specifically, the front end of the mobile phase material inlet 9 is provided with a tee, one of which can be used as a pressure relief port for pressure relief. The pressure relief port is located on the top surface of the column tube 1, so it will not directly hit the observer, making it safer and more reliable.
[0070] The chromatography column is suspended and has four lugs. The four lugs are fixedly connected to the outer side wall of the column tube 1. The main column is suspended on the platform through the lugs on the side of the column tube 1, without occupying ground space.
[0071] Preferably, the chromatography column is made of 304 stainless steel coated with a copolymer of PTFE and ethylene, which can ensure acid and alkali compatibility under operating conditions and also ensure that the outer wall of the column tube 1 will not rust.
[0072] The side wall of column 1 is provided with resin packing inlet 8 and resin packing outlet 7. Resin is filled through resin packing inlet 8 and pumped into the chromatography column. Due to the good fluidity of the resin, waste media can flow out through resin packing outlet 7.
[0073] In one embodiment, an improved low-pressure chromatography column of φ1800*4000 is used. The low-pressure chromatography column with dimensions of φ1800*4000 consists of an upper cover assembly 2, a lower cover assembly 3, and a column tube 1, with a packing height of 3800mm. The upper cover assembly 2 and the lower cover assembly 3 adopt a design with a distributor 5 superimposed on a sieve plate 6, increasing the diameter of the chromatography column to 1800mm. During column packing, a 20cm liquid layer is left above the column bed to still ensure efficient separation of the components in the chromatography. Compared with traditional ion exchange columns, the yield is increased by 10%, wastewater is reduced by 25,000 tons per year, the plant area is only 40% of the original, the equipment investment is only 50% of the original, the labor force after production is only 1 / 3 of the original, and the equipment maintenance cost is also only 1 / 3 of the original.
[0074] By applying the technical solution provided in this embodiment of the invention, the diameter of the chromatography column can be safely increased to 1800 mm, which helps to improve throughput and production scale. Even with the increased column diameter, efficient separation between components during chromatography is still guaranteed, which is essential for maintaining product quality and yield. This facilitates the industrial-scale expansion of the process, reducing plant area requirements, equipment investment, labor requirements, and maintenance workload caused by design limitations. It also helps to reduce wastewater generation, lower the difficulty and cost of wastewater treatment, while improving product purity and yield. Furthermore, it enables a larger-scale, more efficient, and more uniform ion exchange chromatography process, contributing to the industrial upgrading of fermentation broth extraction production lines and environmental sustainability.
[0075] The chromatography column apparatus provided by this invention is used in a fermentation broth extraction production line. The cooperation between the sieve plate 6 and the distributor 5 maximizes the uniformity of fluid distribution and reduces efficiency loss caused by uneven distribution. It helps maintain the synchronization of the entire column bed on the horizontal plane during column loading, washing, and elution. By ensuring uniform fluid distribution and operational synchronization, separation efficiency, production efficiency, and product quality can be improved. It helps reduce the dead volume when the fluid passes through, thereby improving column efficiency. Uniform fluid distribution reduces cross-contamination between samples and improves product purity.
[0076] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.
[0077] The chromatography column apparatus provided by this utility model has been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of this utility model. The descriptions of the embodiments above are only for the purpose of helping to understand the method and core ideas of this utility model. It should be noted that those skilled in the art can make various improvements and modifications to this utility model without departing from its principles, and these improvements and modifications also fall within the protection scope of the claims of this utility model. Therefore, this utility model is not limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A chromatography column apparatus, characterized by, The system includes a column tube (1), an upper cover assembly (2) and a lower cover assembly (3) respectively installed at the upper and lower ends of the column tube (1). The upper cover assembly (2) and the lower cover assembly (3) include a cover plate (4), a distributor (5) and a sieve plate (6). The cover plate (4), the distributor (5) and the sieve plate (6) are distributed sequentially from the outside to the inside. The upper cover plate is provided with a mobile phase material inlet (9), and the lower cover plate is provided with a mobile phase material outlet (10). The distributor (5) includes components connected to the mobile phase material inlet. (9) or the material outlet (53) connected to the mobile phase material outlet (10), multiple flow channels (51) opened on the plate surface and connected to the material outlet (53), and multiple distribution holes (52) provided on the flow channels (51), the flow channels (51) of the upper distributor face the upper cover plate, the flow channels (51) of the lower distributor face the lower sieve plate, the sieve plate (6) includes multiple sieve holes (61) connected to the distribution holes (52), and the diameter of the distribution holes (52) is larger than the diameter of the sieve holes (61).
2. The chromatography column device of claim 1, wherein, The sieve plate (6) has multiple screening areas, with intervals between adjacent screening areas. Each screening area has multiple sieve holes (61). The distributor (5) has multiple distribution areas, with the distribution areas being the same as the vertical projection areas of the screening areas. The number of distribution holes (52) contained in the distribution areas is proportional to the area of the distribution areas.
3. The chromatography column device of claim 2, wherein, A dividing rib is provided between the sieve plate (6) and the distributor (5). One end of the dividing rib abuts against the surface of the distributor (5), and the other end abuts against the surface of the sieve plate (6). The sieve plate (6) is parallel to the distributor (5).
4. The chromatography column device of claim 3, wherein, The sieve plate (6) is integrally formed with the dividing ribs, and the sieve area is divided into independent areas by the dividing ribs.
5. The chromatography column device of claim 1, wherein, The mating surfaces of the distributor (5) and the sieve plate (6) are flat, and the cover plate (4) is in contact with the mating surface of the distributor (5) and connected to the column tube (1) by fasteners.
6. The chromatography column device of claim 5, wherein, The dispenser (5) has a groove on the surface facing the sieve plate (6), the plane is located on the bottom surface of the groove, and the sieve plate (6) is embedded in the groove.
7. The chromatography column device of claim 6, wherein, The depth of the groove is equal to the thickness of the sieve plate (6).
8. The chromatography column device of claim 6, wherein, The groove is fitted with the sieve plate (6) with a clearance. The side wall of the groove or the side wall of the sieve plate (6) has a sealing groove, and a sealing ring is provided in the sealing groove.
9. The chromatography column device of claim 6, wherein, The mounting part of the sieve plate (6) has a through hole, and the bottom surface of the groove is provided with a threaded hole that matches the through hole. The sieve plate (6) and the distributor (5) are connected by a connector.
10. The chromatography column apparatus of claim 7, wherein, The mating surfaces of the sieve plate (6) and the distributor (5) are provided with interlocking concave and convex surfaces.