NK cell culture device

Abstract

The utility model discloses an NK cell culture device which comprises a supporting frame, a first culture bottle, a second culture bottle and a culture bag are sequentially distributed on the supporting frame from top to bottom, an auxiliary support is arranged on the supporting frame, the auxiliary support is rotatably connected with the first culture bottle, a supporting seat is connected to the supporting frame in a sliding mode, and the supporting seat supports the bottom face of the first culture bottle. A liquid outlet is formed in the front side wall of the first culture bottle, a first hose is connected between the liquid outlet and a liquid inlet of the second culture bottle, and a second hose is connected between the second culture bottle and the culture bag. The cell transfer device is novel in structure, does not need to be taken out in the process of transferring cells twice, reduces the risk of pollution, is safe and reliable, reduces the use cost of relevant transfer consumables and instruments, and is easy for experimenters to master and operate.

Description

Technical Field

[0001] This invention belongs to the field of cell culture technology, and particularly relates to an NK cell culture device. Background Technology

[0002] NK cells, or natural killer cells, are important immune cells in the body. They are not only involved in anti-tumor, anti-viral infection, and immune regulation, but also participate in hypersensitivity reactions and the occurrence of autoimmune diseases in certain circumstances. They can recognize target cells and kill mediators.

[0003] Traditional NK cell culture typically involves transferring NK cells from a small number to a large number in a T25 culture flask, then to a T175 culture flask, and finally to a cell culture bag, based on the cell growth rate. During this transfer process, pipettes are used for extraction, which makes the cells susceptible to contamination and requires skilled operators. Furthermore, the numerous consumables and instruments involved contribute to the high cost. Summary of the Invention

[0004] The purpose of this invention is to solve the above-mentioned technical problems existing in the prior art and to provide an NK cell culture device with a novel structure. It eliminates the need for cell removal during the two cell transfer processes, reducing the risk of contamination, ensuring safety and reliability, and lowering the cost of transfer-related consumables and instruments. It is also easy for experimental personnel to operate.

[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0006] An NK cell culture device includes: a support frame, on which a first culture flask, a second culture flask, and a culture bag are arranged sequentially from top to bottom. The device is characterized by: an auxiliary support on the support frame, the auxiliary support being rotatably connected to the first culture flask; a support base slidably connected to the support frame, the support base supporting the bottom surface of the first culture flask; an outlet on the front side wall of the first culture flask; a first flexible tube connecting the outlet to the inlet of the second culture flask; and a second flexible tube connecting the second culture flask to the culture bag. This culture device has a novel structure, eliminating the need for removal operations during two cell transfer processes, reducing the risk of contamination, ensuring safety and reliability, and lowering the cost of culture-related consumables and instruments. It is also easy for researchers to operate.

[0007] Furthermore, the support frame is equipped with hooks, and the culture bags have ears with corresponding hanging holes. The culture bags are hung by hooking them, making loading and unloading convenient and improving usability.

[0008] Furthermore, the support frame is equipped with a support platform, and the support platform has a limiting groove corresponding to the second culture flask. The limiting groove has a fixing through hole for the drain port at the bottom of the second culture flask to pass through. The limiting groove can restrict the lateral movement of the second culture flask, thereby improving the placement stability of the second culture flask.

[0009] Furthermore, the support platform is equipped with a barrier to prevent the second culture flask from tipping over freely. When the second culture flask tipps over due to an accidental impact, the barrier can provide support to the second culture flask and prevent it from falling directly off the support platform.

[0010] Furthermore, the auxiliary support is equipped with a positioning block to prevent the first culture flask from tilting. When the first culture flask is rotated backward to contact the positioning block, the tilting angle of the second culture flask is optimal, which facilitates the rapid outflow of liquid from the outlet of the first culture flask and reduces the difficulty of operation for the experimenter.

[0011] Furthermore, one end of the first hose is provided with a first connector, and an outer ring is provided outside the liquid outlet. An annular groove is formed between the outer ring and the liquid outlet, and an internal thread is provided in the annular groove to engage with the external thread of the first connector. After the first connector is connected, the first connector enters the annular groove, which extends the sealing path, provides good sealing performance, prevents liquid from leaking out from the connection between the liquid outlet and the first connector, ensures reliable connection, facilitates disassembly, and makes it easy to replace the first hose.

[0012] Furthermore, one end of the second tubing is provided with a second connector that is screwed into the drain port at the bottom of the second culture flask, ensuring reliable connection, good sealing, easy disassembly, and convenient replacement of the second tubing.

[0013] Furthermore, a flow regulator is connected to the second tubing. When the flow regulator is closed, the second tubing is completely closed, and the liquid will not flow into the culture bag; when the flow regulator is open, the flow rate of the liquid in the second tubing can be flexibly adjusted, making it flexible and convenient.

[0014] This utility model, by adopting the above-mentioned technical solution, has the following beneficial effects:

[0015] When transferring cells from the first culture flask, first slide the support horizontally to remove it from contact with the first culture flask, then rotate the first culture flask backward so that the outlet faces downward. The liquid in the first culture flask flows out from the outlet and into the second culture flask through the first tubing. When transferring cells from the second culture flask, turn on the flow regulator. The liquid in the second culture flask flows out from the drain outlet and into the culture bag through the second tubing. No removal is required during the two cell transfers, reducing the risk of contamination, ensuring safety and reliability, and lowering the cost of transfer-related consumables and instruments. The operation is also easy for researchers to learn. Attached Figure Description

[0016] The present invention will be further described below with reference to the accompanying drawings:

[0017] Figure 1 This is a schematic diagram of the structure of an NK cell culture device according to the present invention;

[0018] Figure 2 This is a schematic diagram showing the first culture flask in an inverted state in this utility model;

[0019] Figure 3 for Figure 1 The main view;

[0020] Figure 4 This is a schematic diagram of the connection between the second culture flask and the culture bag in this utility model;

[0021] Figure 5 This is a schematic diagram of the support platform in this utility model;

[0022] Figure 6 This is a schematic diagram of the connection between the first culture flask and the first tubing in this utility model;

[0023] Figure 7 This is a schematic diagram of the structure of the second culture flask in this utility model;

[0024] Figure 8 This is a schematic diagram of the connection between the second hose and the flow regulator in this utility model.

[0025] In the diagram: 1-Support frame; 2-First culture flask; 3-Second culture flask; 4-Culture bag; 5-Support platform; 6-Limiting groove; 7-Fixing through hole; 8-Enclosure part; 9-Hook; 10-Ear part; 11-Auxiliary bracket; 12-Support base; 13-Outlet; 14-First hose; 15-Second hose; 16-U-shaped rod; 17-Positioning block; 18-First connector; 19-Outer ring part; 20-Ring groove; 21-Second connector; 22-Drainage port; 23-Flow regulator. Detailed Implementation

[0026] like Figures 1 to 8 As shown, this utility model discloses an NK cell culture device, comprising: a support frame 1, wherein a first culture flask 2, a second culture flask 3, and a culture bag 4 are arranged sequentially from top to bottom on the support frame 1; a support platform 5 is provided on the support frame 1; a limiting groove 6 corresponding to the second culture flask 3 is provided on the support platform 5; a fixing through hole 7 is provided in the limiting groove 6 for the drainage port 22 at the bottom of the second culture flask 3 to pass through; the limiting groove 6 can restrict the lateral movement of the second culture flask 3, thereby improving the placement stability of the second culture flask 3.

[0027] The support platform 5 is provided with a barrier 8, which is used to prevent the second culture bottle 3 from tipping over freely. When the second culture bottle 3 tipps over due to an accidental impact, the barrier 8 can provide support for the second culture bottle 3 and prevent the second culture bottle 3 from falling directly from the support platform 5.

[0028] Hooks 9 are symmetrically distributed on the bottom surface of the support platform 5, and the culture bag 4 is provided with ears 10, with hanging holes corresponding to the hooks 9. The culture bag 4 is hung by hooking it with the hooks 9, making it easy to load and unload and improving convenience.

[0029] The first culture flask 2 is wider at the top and narrower at the bottom. NK cell culture medium is injected into the inlet of the first culture flask 2, and then a cap with a vent is placed on the inlet of the first culture flask 2. This is existing technology and will not be described in detail here.

[0030] The support frame 1 is provided with an auxiliary support 11, which is rotatably connected to the first culture flask 2. The support frame 1 is slidably connected with a support base 12, which supports the bottom surface of the first culture flask 2, so that the first culture flask 2 can be placed stably.

[0031] The first culture flask 2 has an outlet 13 on its front side wall. A first hose 14 is connected between the outlet 13 and the inlet of the second culture flask 3. A second hose 15 is connected between the second culture flask 3 and the culture bag 4.

[0032] One end of the first hose 14 is provided with a first connector 18, and an outer ring 19 is provided outside the outlet 13. An annular groove 20 is formed between the outer ring 19 and the outlet 13. An internal thread is provided in the annular groove 20 to engage with the external thread of the first connector 18.

[0033] After the first connector 18 is connected, the first connector 18 enters the annular groove 20, which extends the sealing path, provides good sealing performance, and prevents liquid from leaking out from the connection between the outlet 13 and the first connector 18. The connection is reliable, disassembly is convenient, and the first hose 14 is easy to replace.

[0034] One end of the second hose 15 is provided with a second connector 21. The second connector 21 has an internal thread that is screwed into the external thread of the drain port 22. The threaded connection is reliable, has good sealing performance, is easy to disassemble, and is convenient to replace the second hose 15.

[0035] A flow regulator 23 is connected to the second tubing 15. When the flow regulator 23 is closed, the second tubing 15 is completely closed by the pulley, preventing liquid from flowing into the culture bag 4. When the flow regulator 23 is open, manually pushing the pulley to squeeze the second tubing 15 can change the flow rate of the liquid inside the second tubing 15, making adjustment convenient. The structure of the flow regulator 23 is prior art and will not be described in detail here.

[0036] When it is necessary to transfer cells from the first culture flask 2, first slide the support base 12 horizontally so that it is no longer in contact with the first culture flask 2, then rotate the first culture flask 2 backward so that the outlet 13 faces downward. The liquid in the first culture flask 2 flows out from the outlet 13 and into the second culture flask 3 through the first tubing 14. When it is necessary to transfer cells from the second culture flask 3, turn on the flow regulator 23. The liquid in the second culture flask 3 flows out from the drain 22 and into the culture bag 4 through the second tubing 15. There is no need to remove the cells during the two cell transfer processes, which reduces the risk of contamination, is safe and reliable, and reduces the cost of transfer-related consumables and instruments. It is also easy for experimental personnel to operate.

[0037] The auxiliary support 11 is provided with a U-shaped rod 16, and a positioning block 17 is provided on the bottom surface of the U-shaped rod 16. The positioning block 17 is located on the rear side of the first culture bottle 2.

[0038] When the first culture flask 2 is rotated backward to contact the positioning block 17, the tilting angle of the second culture flask 3 is optimal, which facilitates the rapid flow of liquid from the outlet 13 in the first culture flask 2, reducing the difficulty of operation for the experimenter; the positioning block 17 is made of rubber to prevent cracks from occurring after the first culture flask 2 collides with the positioning block 17.

[0039] The first culture flask 2 has a volume of 5-7 ml, the second culture flask 3 has a volume of 15-20 ml, and the culture bag 4 has a volume of 1.8 L.

[0040] The above are merely specific embodiments of this utility model, but the technical features of this utility model are not limited thereto. Any simple changes, equivalent substitutions, or modifications made based on this utility model to solve essentially the same technical problems and achieve essentially the same technical effects are all covered within the protection scope of this utility model.

Claims

1. An NK cell culture device, comprising: A support frame, wherein a first culture flask, a second culture flask, and a culture bag are arranged sequentially from top to bottom. Its features are: The support frame is provided with an auxiliary bracket, which is rotatably connected to the first culture flask. A support base is slidably connected to the support frame, which supports the bottom surface of the first culture flask. The front side wall of the first culture flask is provided with a liquid outlet. A first hose is connected between the liquid outlet and the liquid inlet of the second culture flask. A second hose is connected between the second culture flask and the culture bag.

2. The NK cell culture device of claim 1, wherein: The support frame is provided with hooks, and the culture bag is provided with ears, with hanging holes corresponding to the hooks on the ears.

3. The NK cell culture device of claim 1, wherein: The support frame is provided with a support platform, and the support platform is provided with a limiting groove corresponding to the second culture flask. The limiting groove is provided with a fixing through hole for the drain port at the bottom of the second culture flask to pass through.

4. The NK cell culture device of claim 3, wherein: The support platform is provided with a barrier to prevent the second culture flask from tipping over freely.

5. The NK cell culture device of claim 1, wherein: The auxiliary support is equipped with a positioning block to block the first culture flask.

6. The NK cell culture device of claim 1, wherein: One end of the first hose is provided with a first connector, and an outer ring is provided outside the liquid outlet. An annular groove is formed between the outer ring and the liquid outlet. An internal thread is provided in the annular groove to engage with the external thread of the first connector.

7. The NK cell culture device of claim 1, wherein: One end of the second tubing is provided with a second connector that is screwed into the drain port at the bottom of the second culture flask.

8. The NK cell culture device of claim 1, wherein: A flow regulator is connected to the second hose.

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