Colorectal cancer micro-tumor cell culture device

Abstract

The utility model discloses a colorectal cancer micro tumor cell culture device which comprises a culture dish and a sealing cover, the sealing cover covers and presses the top of the culture dish, the outer walls of the culture dish and the sealing cover are sleeved with two semi-arc-shaped cladding plates, the interiors of the two semi-arc-shaped cladding plates are of a cavity structure, and the two semi-arc-shaped cladding plates are arranged in the cavity structure. And the internal cavities of the two semi-arc cladding plates are filled with purified water. According to the utility model, purified water filled in the semi-arc-shaped cladding plate is used as a high-specific-heat-capacity heating medium, and a closed-loop temperature control system constructed by the electric heating wire and the top thermometer is combined, so that the temperature of the inner cavity of the culture dish can be continuously maintained after the culture dish is separated from an incubator environment, and the interference of external environment temperature fluctuation is effectively resisted; the method is especially suitable for cross-laboratory cooperation or in-situ detection scenes requiring frequent sample transfer; through the collaborative design of the arc-shaped through groove and temperature control, the proper temperature can be ensured while the colorectal cancer cells are observed, and temperature loss is avoided.

Description

Technical Field

[0001] This utility model relates to the field of cell culture equipment technology, and more specifically, to a colorectal cancer microtumor cell culture device. Background Technology

[0002] In studies on tumor microenvironment and drug sensitivity, three-dimensional culture of colorectal cancer microtumor cells needs to be carried out under isothermal conditions that simulate the in vivo microenvironment.

[0003] Traditional culture dishes rely on incubators to maintain a constant temperature of 37°C. Once removed from the incubator (such as for inter-laboratory transport, in-situ microscopic detection, or immediate sample analysis), fluctuations in the ambient temperature will cause a sudden drop in the culture medium temperature, which will interfere with the culture of colon cancer cells, causing stress to the colon cancer cells and thus causing experimental data to deviate from the true biological characteristics.

[0004] Therefore, this application proposes a colorectal cancer microtumor cell culture device to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to provide a colorectal cancer microtumor cell culture device to address the aforementioned shortcomings in the technology.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A colorectal cancer microtumor cell culture device includes a culture dish and a sealing cap. The sealing cap is placed on top of the culture dish. Two semi-circular covering plates are sleeved on the outer wall of the culture dish and the sealing cap. The interior of the two semi-circular covering plates is hollow. The interior cavities of the two semi-circular covering plates are filled with purified water. Heating wires are installed in the interior cavities of the two semi-circular covering plates. Arc-shaped through grooves are opened at the corresponding central positions of the two semi-circular covering plates. The two arc-shaped through grooves are combined to form a circular structure.

[0008] Preferably, the top outer wall of the culture dish is provided with a sealing ring, and the bottom inner wall of the sealing cap is provided with a sealing groove, the inner wall contour of the sealing groove being adapted to the outer wall contour of the sealing ring.

[0009] Preferably, a docking block is fixedly provided on one side of the outer wall of the two semi-circular covering plates, and a permanent magnet is fixedly provided on one side of the outer wall of the docking block through a slot, and the outer walls of the corresponding permanent magnets are attracted to each other.

[0010] Preferably, the top of the two semi-circular covering plates is respectively fixed with a liquid inlet pipe and a vent pipe through an opening. The liquid inlet pipe and the vent pipe are respectively connected to the cavity structure provided inside the semi-circular covering plate. The top outer wall of the liquid inlet pipe and the vent pipe are equipped with a matching thermometer.

[0011] Preferably, a thermometer is installed on the top outer wall of the two semi-circular covering plates, and the detection end of the thermometer is located inside the cavity.

[0012] Preferably, a power interface is installed on the outer wall of one corresponding side of the two semi-circular covering plates.

[0013] Preferably, rubber pads are adhered to the bottom outer walls of the two semi-circular covering plates.

[0014] The technical effects and advantages provided by this utility model in the above technical solution are as follows:

[0015] 1. The culture device of this utility model uses pure water filled inside the semi-arc-shaped covering plate as a high specific heat capacity heat medium, combined with a closed-loop temperature control system constructed by heating wire and top thermometer. It can continuously maintain the temperature inside the culture dish after leaving the incubator environment, effectively resisting the interference of external environmental temperature fluctuations. It is especially suitable for cross-laboratory collaboration or in-situ detection scenarios that require frequent sample transfer.

[0016] 2. The synergistic design of the arc-shaped through groove and temperature control in this invention can ensure a suitable temperature while observing colorectal cancer cells, avoid temperature loss, and improve the stability of cell experimental data. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0018] Figure 1 This is a schematic diagram of the unfolded structure of a colorectal cancer microtumor cell culture device according to the present invention;

[0019] Figure 2 This is a schematic diagram of the culture dish structure of a colorectal cancer microtumor cell culture device according to the present invention;

[0020] Figure 3 This is a schematic diagram of the semi-arc-shaped covering plate structure of a colorectal cancer microtumor cell culture device according to the present invention;

[0021] Figure 4 This is a schematic diagram of the cross-sectional structure of the semi-arc-shaped covering plate of the colorectal cancer microtumor cell culture device of this utility model;

[0022] Figure 5 This is a schematic diagram of the semi-arc-shaped covering plate assembly structure of a colorectal cancer microtumor cell culture device according to the present invention.

[0023] Explanation of reference numerals in the attached figures:

[0024] 1. Petri dish, 2. Sealing cap, 3. Semi-circular covering plate, 4. Connecting block, 5. Permanent magnet, 6. Heating wire, 7. Liquid addition tube, 8. Exhaust tube, 9. Thermometer, 10. Rubber pad, 11. Power interface, 12. Arc-shaped through groove. Detailed Implementation

[0025] The following drawings will disclose several embodiments of this utility model. For clarity, many physical details will be described in the following description. However, it should be understood that these physical details should not be used to limit this utility model. That is, in some embodiments of this utility model, these physical details are not essential. In addition, for the sake of simplicity, some conventional structures and components will be shown in the drawings in a simple schematic manner.

[0026] Furthermore, in this utility model, the use of terms such as "first" and "second" is for descriptive purposes only and does not specifically refer to any order or sequence, nor is it intended to limit the utility model. They are merely used to distinguish components or operations described with the same technical terms and should not be construed as indicating or implying their relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions of various embodiments can be combined with each other, but only if they are feasible for those skilled in the art. If a combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0027] Example 1: Refer to Figures 1-5 As shown, the present embodiment provides a colorectal cancer microtumor cell culture device, which mainly consists of a culture dish 1, a sealing cap 2, and two semi-circular covering plates 3. The culture dish 1 is placed stably, and the sealing cap 2 is placed on top of the culture dish 1. The outer wall of the top of the culture dish 1 is provided with a sealing ring, and the inner wall of the bottom of the sealing cap 2 is provided with a matching sealing groove. Through the tight fit between the sealing ring and the sealing groove, the sealing between the culture dish 1 and the sealing cap 2 can be effectively enhanced, preventing external impurities from entering the culture environment and providing a stable and clean culture space for colorectal cancer microtumor cells.

[0028] Example 2: Based on Example 1, in this example, two semi-circular covering plates 3 are then installed and fitted onto the outer walls of the culture dish 1 and the sealing cap 2. The two semi-circular covering plates 3 have a hollow internal structure. Before assembly, the cavities are filled with purified water, which acts as a heat conduction medium, facilitating the uniform transfer of heat. A heating wire 6 is also installed within the cavity. When energized, the heating wire 6 generates heat, which is then uniformly conducted to the culture dish 1 through the purified water, providing a suitable culture temperature for colorectal cancer microtumor cells.

[0029] Example 3: Based on Example 1, the arc-shaped through groove 12 in the center of the two semi-arc-shaped covering plates 3 in this example form an annular observation channel. This channel is coaxial with the inner cavity of the petri dish 1, and its width is adapted to the outer diameter of a common microscope objective. A docking block 4 is fixedly installed on the outer wall of the corresponding side of the two semi-arc-shaped covering plates 3. A permanent magnet 5 is fixedly installed on the outer wall of the docking block 4 through a groove. When the two semi-arc-shaped covering plates 3 are close to each other, the outer walls of the corresponding permanent magnets 5 attract each other, so that the two semi-arc-shaped covering plates 3 are tightly connected.

[0030] Example 4: Based on Example 1, to facilitate operation and monitoring of the internal cavity of the semi-circular covering plate 3, a liquid inlet pipe 7 and an exhaust pipe 8 are fixedly installed on the top of the two semi-circular covering plates 3 through openings, respectively. The liquid inlet pipe 7 and the exhaust pipe 8 are connected to the internal cavity structure of the semi-circular covering plate 3. When it is necessary to add or replace purified water into the cavity, it can be done through the liquid inlet pipe 7; the exhaust pipe 8 is used to discharge gas from the cavity to prevent excessive internal pressure. At the same time, a matching thermometer 9 is provided on the top outer wall of the liquid inlet pipe 7 and the exhaust pipe 8. The temperature of the liquid flowing through the liquid inlet pipe 7 and the exhaust pipe 8 can be monitored in real time through the thermometer 9, indirectly understanding the temperature of the liquid in the cavity. In addition, thermometers 9 are also installed on the top outer wall of the two semi-circular covering plates 3. The detection end of the thermometer 9 is located inside the cavity, which can directly and accurately measure the temperature of the liquid in the cavity, so as to adjust the heating power of the heating wire 6 in a timely manner and stabilize the culture temperature within the suitable range for the growth of colorectal cancer microtumor cells.

[0031] Example 5: Based on Example 1, in this example, a power interface 11 is installed on the outer wall of one side of the two semi-circular covering plates 3. The power interface 11 is connected to an external power source to provide stable power support for the heating wire 6. Rubber pads 10 are adhered to the bottom outer wall of the two semi-circular covering plates 3. The rubber pads 10 can play a role in buffering and anti-slip, so as to avoid the device from being affected by vibration or sliding during placement.

[0032] Working principle:

[0033] Reference Figures 1-5As shown, in use, the culture medium containing colorectal cancer microtumor cells is injected into the culture dish 1 and the sealing cap 2 is tightened. The sealing ring and the sealing groove are fitted together to form a sterile closed cavity. Then, two semi-arc-shaped covering plates 3 pre-filled with pure water and with built-in heating wires 6 are symmetrically fastened to the outer wall of the assembly. The magnetic attraction of the permanent magnet 5 on the docking block 4 makes the two covering plates fit tightly together. The arc-shaped through groove 12 is automatically aligned to form an annular observation channel. The inner diameter of the channel is adapted to the microscope objective lens, and the objective lens can penetrate unobstructed to the bottom glass of the culture dish 1.

[0034] The power interface 11 is connected to activate the heating wire 6. Pure water serves as the heat medium, evenly transferring heat to the culture dish 1. A top thermometer 9 monitors the temperature in real-time, maintaining a constant environment of 37℃±0.5℃. A bottom rubber pad 10 cushions the vibrations from the microscopic focusing. During culture, the microscope objective lens directly contacts the bottom of the culture dish 1 through the arc-shaped through-slot 12, allowing for in-situ dynamic observation of the tumor spheres using transmitted light or phase contrast mode. Cell morphology, proliferation rate, and changes in the invasion front are recorded simultaneously. After the experiment, the power is turned off, the coating plate 3 is separated, and the culture dish 1 is removed for cell harvesting or fixation, completing a single culture-observation cycle. This device integrates thermal control and observation functions through the arc-shaped through-slot 12, overcoming the mutual limitations of temperature maintenance and microscopic monitoring in traditional culture devices. It provides a continuous observation platform for studying the heterogeneity and drug sensitivity of colorectal cancer tumors.

[0035] The foregoing description only illustrates certain exemplary embodiments of the present invention. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A colorectal cancer microtumor cell culture device comprising a culture dish (1) under a sealing cover (2), said sealing cover (2) being pressed on top of the culture dish (1), characterized in that, The culture dish (1) and the outer wall of the sealing cap (2) are fitted with two semi-arc-shaped covering plates (3). The interior of the two semi-arc-shaped covering plates (3) is hollow. The interior cavities of the two semi-arc-shaped covering plates (3) are filled with pure water. The interior cavities of the two semi-arc-shaped covering plates (3) are provided with heating wires (6). The two semi-arc-shaped covering plates (3) are respectively provided with arc-shaped through grooves (12) at their central positions. The two arc-shaped through grooves (12) are combined to form a circular structure.

2. The colorectal cancer micrometastasis cell culture device of claim 1, wherein, The top outer wall of the culture dish (1) is provided with a sealing ring, and the bottom inner wall of the sealing cover (2) is provided with a sealing groove, the inner wall contour of the sealing groove being adapted to the outer wall contour of the sealing ring.

3. The colorectal cancer micrometastasis cell culture device of claim 1, wherein, A docking block (4) is fixedly provided on one side of the outer wall of the two semi-circular covering plates (3). A permanent magnet (5) is fixedly provided on one side of the outer wall of the docking block (4) through a slot. The outer walls of the corresponding permanent magnets (5) are attracted to each other.

4. The colorectal cancer micrometastasis cell culture device of claim 1, wherein, The top of the two semi-circular covering plates (3) are respectively fixed with a liquid filling pipe (7) and an exhaust pipe (8) through openings. The liquid filling pipe (7) and the exhaust pipe (8) are respectively connected to the cavity structure provided inside the semi-circular covering plate (3). The top outer wall of the liquid filling pipe (7) and the exhaust pipe (8) are equipped with a matching thermometer (9).

5. The colorectal cancer micrometastasis cell culture device of claim 1, wherein, Thermometers (9) are installed on the top outer walls of the two semi-circular covering plates (3), and the detection end of the thermometers (9) is located inside the cavity.

6. The colorectal cancer micrometastasis cell culture device of claim 1, wherein, A power interface (11) is installed on the outer wall of one side of each of the two semi-circular covering plates (3).

7. The colorectal cancer micrometastasis cell culture device of claim 1, wherein, Rubber pads (10) are bonded to the bottom outer walls of the two semi-circular covering plates (3).

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