Raw material dissolving device for preparing cell culture medium

Abstract

The utility model relates to the field of cell culture media, in particular to a raw material dissolving device for preparing a cell culture medium, which comprises a dissolving barrel, a main body for bearing raw materials and a sealing cover, the sealing cover is fixedly arranged above the dissolving barrel and is used for sealing a dissolving space, and rotating shafts are fixedly mounted on two sides of the outer part of the dissolving barrel. The raw material dissolving device for preparing the cell culture medium is provided with the dissolving barrel, the adjusting frame, the angle frame, the pushing frame, the telescopic air cylinder, the steam box, the steam ring, the multi-hole spray head, the dissolving wall knife and the striving rotating cylinder, when the raw material dissolving device for preparing the cell culture medium is used, raw materials in the dissolving barrel are not stirred and dissolved at a fixed angle, and the raw materials can be stirred and dissolved in the stirring process; along with stretching and retracting of the telescopic air cylinder, the dissolving barrel is driven by the pushing frame and the angle frame to conduct angle inclination on the adjusting frame, then quantitative dissolving liquid can be injected into the steam box, an electric heating pipe heats the dissolving liquid through the interval heat conduction plate, and the dissolving liquid is heated to rise into steam.

Description

Technical Field

[0001] This utility model relates to the field of cell culture media, and specifically to a raw material dissolution device for preparing cell culture media. Background Technology

[0002] Cell culture medium is an artificial nutrient environment that simulates the growth of cells in vivo. It is used to provide the nutrients required for cell survival, growth and reproduction. It is the foundation of cell culture and directly affects the growth status of cells and the reliability of experimental results. The raw material dissolving device for cell culture medium preparation is used to dissolve and mix various raw materials such as amino acids, vitamins, inorganic salts, carbon sources, serum, growth factors and so on evenly during the preparation of cell culture medium.

[0003] A search revealed a device for dissolving raw materials for serum-free cell culture medium, with publication number CN221062496U. Specifically, this device includes a housing and a motor. The motor is fixedly connected to the upper end of the housing, and a first gear is fixedly connected to the outer side of the motor's main shaft. The outer side of the first gear meshes with the outer side of a rotating column. A ventilation shell is rotatably connected to the upper end of the rotating column, and a fan is fixedly connected to one side of the ventilation shell. A material box is fixedly connected to one side of the fan, and a first rotating wheel is fixedly connected to the outer side of the motor's main shaft. In this invention, the fan conveys the raw materials, and the fan drives the device to rotate under air pressure, thereby driving the spiral blades to rotate. While conveying the raw materials, the device simultaneously crushes any agglomerated material. The raw materials fall evenly from the outlet, achieving rapid and uniform dissolution with the solution. Simultaneously, the solution is stirred, and agglomerated raw materials are broken up by the device, improving the dissolution effect.

[0004] Existing cell culture medium preparation raw material dissolution devices suffer from low dissolution efficiency due to restricted flow and mixing of raw materials within the dissolution tank, resulting in increased dead zones. This makes the overall dissolution process inadequate. Furthermore, the cell culture medium preparation raw material dissolution devices in the aforementioned comparative case also lack methods to improve the completeness of raw material dissolution. Long-term use can lead to uneven dispersion of raw materials in the solvent, resulting in unstable dissolution quality and damaging cell viability.

[0005] Therefore, it is necessary to invent a raw material dissolution device for cell culture medium preparation to solve the above problems. Utility Model Content

[0006] The purpose of this invention is to provide a raw material dissolving device for cell culture medium preparation. Through a dissolving tank, adjusting frame, angle frame, pushing frame, telescopic cylinder, steam box, steam ring, multi-hole nozzle, dissolving wall blade, and rotating drum, the device achieves more comprehensive dissolution of raw materials, promoting mixing and reducing dead zones. The assistance of dissolving steam, through heat and kinetic energy, further accelerates dissolution and improves dissolution quality. This synergistic operation ensures both macroscopic agitation and microscopic mixing during the stirring process, improving dissolution efficiency and helping to reduce shear forces on cells during stirring. This invention aims to protect cell viability and address the shortcomings of existing cell culture medium preparation raw material dissolution devices. In these devices, the flow and mixing of raw materials within the dissolution tank are restricted, leading to increased dead zones and low dissolution efficiency. Consequently, the overall dissolution of cell culture medium is incomplete. Furthermore, the aforementioned comparative case also lacks methods to enhance the comprehensiveness of raw material dissolution. Long-term use can result in uneven dispersion of raw materials in the solvent, leading to unstable dissolution quality and damage to cell viability.

[0007] To achieve the above objectives, this utility model provides the following technical solution: a raw material dissolving device for preparing cell culture medium, comprising a dissolving tank and a main body for carrying the raw materials;

[0008] A sealing cap is fixedly installed above the dissolving tank to seal the dissolving space. Rotating shafts are fixedly installed on both sides of the outside of the dissolving tank. An adjusting frame is rotatably connected to the outside of the rotating shafts. An angle frame is threadedly connected to the outside of the dissolving tank. A pushing frame is fixedly installed at the bottom of the angle frame. A telescopic cylinder is fixedly installed on the outside of the adjusting frame.

[0009] A steam box is fixedly installed outside the dissolving tank. It has an external injection port and a spacer heat-conducting plate fixedly installed inside the steam box. An electric heating tube is fixedly installed at the bottom of each spacer heat-conducting plate. A steam filter is fixedly installed inside the steam box. A high-temperature resistant air pump is fixedly installed on the top of the steam box. A suction pipe is fixedly installed at the output end of the high-temperature resistant air pump. A steam ring is fixedly installed at the other end of the suction pipe. A multi-hole nozzle is fixedly installed on the outside of the steam ring.

[0010] A servo motor is fixedly mounted above the sealing cover. A stirring rod is fixedly installed at the output end, and a dissolving wall blade is fixedly installed on the outside of the stirring rod. A flowing drum is fixedly installed at the bottom of the stirring rod, and a jet hole is opened on the outside of the flowing drum.

[0011] Preferably, the front end of the telescopic cylinder is hinged to the bottom of the push frame, and the dissolving tank is rotatably connected to the adjustment frame.

[0012] Preferably, the steam filter is fixedly disposed above the spacer heat-conducting plate, and the steam ring is fixedly installed inside the dissolving tank.

[0013] Preferably, the number of the porous nozzles is set to multiple, and the multiple porous nozzles are distributed in a ring array on the steam ring.

[0014] Preferably, the dissolving wall blade is rotatably connected to the dissolving tank, and the rotating drum and the stirring rod are vertically distributed.

[0015] Preferably, an inner sealing ring is fixedly installed inside the sealing cover, a feeding pipe is fixedly installed above the sealing cover, a fixing ring is fixedly installed inside the feeding pipe, and a slitting blade is fixedly installed inside each fixing ring.

[0016] The technical effects and advantages provided by this utility model in the above technical solution are as follows:

[0017] This invention comprises a dissolving tank, an adjusting frame, an angle frame, a pushing frame, a telescopic cylinder, a steam box, a steam ring, a multi-hole nozzle, a dissolving wall blade, and a rotating drum. When using this raw material dissolving device for cell culture medium preparation, the raw materials inside the dissolving tank are not fixed at a single angle for stirring and dissolving. During the stirring process, as the telescopic cylinder extends and retracts, the pushing frame and angle frame cause the dissolving tank to tilt at an angle on the adjusting frame. Then, a measured amount of dissolving liquid can be injected into the steam box, allowing the electric heating tube to heat the dissolving liquid through the spaced heat-conducting plates, causing the dissolving liquid to be heated and rise into steam. The high-temperature resistant air pump then draws the dissolved steam and sprays it through the steam ring from the multi-hole nozzle. The device, in conjunction with rotating dissolving blades and a flowing drum, accelerates the dissolution of raw materials inside the dissolving tank. The vertically distributed blades and drum agitate the raw materials above and rotate and flow them below. This design allows for more comprehensive dissolution of the raw materials used in the cell culture medium preparation, promoting mixing and reducing dead zones. The auxiliary dissolving steam, through heat and kinetic energy, further accelerates dissolution and improves dissolution quality. This synergistic operation provides both macroscopic agitation and microscopic mixing during the stirring process, increasing dissolution efficiency and reducing shear forces on cells, thus protecting cell viability. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0019] Figure 1This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the adjustment frame structure of this utility model;

[0021] Figure 3 This is a schematic diagram of the steam box structure of this utility model;

[0022] Figure 4 This is a schematic diagram of the steam coil structure of this utility model;

[0023] Figure 5 This is a schematic diagram of the dissolving wall blade structure of this utility model;

[0024] Figure 6 This is a schematic diagram of the feeding tube structure of this utility model.

[0025] Explanation of reference numerals in the attached figures:

[0026] 1. Dissolving tank; 2. Sealing cap; 3. Rotating shaft; 4. Adjusting frame; 5. Angle frame; 6. Pushing frame; 7. Telescopic cylinder; 8. Steam box; 9. Injection port; 10. Spacer heat-conducting plate; 11. Electric heating tube; 12. Steam filter; 13. High-temperature resistant air pump; 14. Air extraction pipe; 15. Steam ring; 16. Multi-hole nozzle; 17. Servo motor; 18. Stirring rod; 19. Dissolving wall blade; 20. Flowing drum; 21. Spray hole; 22. Inner sealing ring; 23. Feeding pipe; 24. Fixing ring; 25. Slitting blade. Detailed Implementation

[0027] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings.

[0028] This utility model provides, for example Figure 1-6 The apparatus shown is a raw material dissolving device for preparing cell culture medium, including a dissolving tank 1, which is a main body for holding the raw materials;

[0029] A sealing cap 2 is fixedly installed above the dissolving tank 1 to seal the dissolving space. A rotating shaft 3 is fixedly installed on both sides of the outside of the dissolving tank 1. An adjusting frame 4 is rotatably connected to the outside of the rotating shaft 3. An angle frame 5 is threadedly connected to the outside of the dissolving tank 1. A pushing frame 6 is fixedly installed at the bottom of the angle frame 5. A telescopic cylinder 7 is fixedly installed on the outside of the adjusting frame 4.

[0030] A steam box 8 is fixedly installed outside the dissolving tank 1. An injection port 9 is fixedly opened on the outside of the steam box 8. A spacer heat-conducting plate 10 is fixedly installed inside the steam box 8. An electric heating tube 11 is fixedly installed at the bottom of each spacer heat-conducting plate 10. A steam filter screen 12 is fixedly installed inside the steam box 8. A high-temperature resistant air pump 13 is fixedly installed on the top of the steam box 8. A suction pipe 14 is fixedly installed at the output end of the high-temperature resistant air pump 13. A steam ring 15 is fixedly installed at the other end of the suction pipe 14. A multi-hole nozzle 16 is fixedly installed on the outside of the steam ring 15.

[0031] A servo motor 17 is fixedly mounted above the sealing cover 2. A stirring rod 18 is fixedly mounted at the output end, and a dissolving wall blade 19 is fixedly mounted on the outside of the stirring rod 18. A flowing drum 20 is fixedly mounted at the bottom of the stirring rod 18. The flowing drum 20 has spray holes 21 on the outside. A high-temperature resistant air pump 13 draws dissolving steam and sprays it out from the multi-hole nozzle 16 through the steam ring 15. With the rotating dissolving wall blade 19 and the flowing drum 20, the raw materials inside the dissolving tank 1 are dissolved more quickly. The dissolving wall blade 19 and the flowing drum 20 are vertically distributed. The upper part stirs the raw materials, and the lower part rotates and flows the dispersed raw materials. This design makes the raw material dissolving device for cell culture medium preparation more comprehensive in dissolving raw materials.

[0032] like Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, the front end of the telescopic cylinder 7 is hinged to the bottom of the push frame 6, and the dissolving tank 1 is rotatably connected to the adjustment frame 4. The raw materials inside the dissolving tank 1 are not fixed at one angle for stirring and dissolving. During the stirring process, as the telescopic cylinder 7 extends and retracts, the push frame 6 and the angle frame 5 drive the dissolving tank 1 to tilt at an angle on the adjustment frame 4. The steam filter 12 is fixedly set above the spacer heat-conducting plate 10, and the steam ring 15 is fixedly installed inside the dissolving tank 1. A certain amount of dissolving liquid can be injected into the steam box 8, allowing the electric heating tube 11 to heat the dissolving liquid through the spacer heat-conducting plate 10, so that the dissolving liquid is heated and rises into steam, thereby assisting in dissolution. The number of multi-hole nozzles 16 is set to multiple, and the multiple multi-hole nozzles 16 are distributed in a ring array on the steam ring 15. The multi-hole nozzles 16 can be inside the dissolving tank 1, so that the dissolving steam can be evenly sprayed on the raw materials to mix with the raw materials and assist in dissolution.

[0033] like Figure 1 , Figure 5 and Figure 6As shown, the dissolving wall blade 19 is rotatably connected to the dissolving tank 1, and the flowing drum 20 and the stirring rod 18 are vertically distributed. The dissolving wall blade 19 and the flowing drum 20 are vertically distributed, stirring the raw materials above and rotating and flowing the dispersed raw materials below. An inner sealing ring 22 is fixedly installed inside the sealing cover 2, and a feeding pipe 23 is fixedly installed above the sealing cover 2. A fixing ring 24 is fixedly installed inside the feeding pipe 23, and a cutting blade 25 is fixedly installed inside each fixing ring 24. The fixing ring 24 and the cutting blade 25 in the feeding pipe 23 can cut the raw materials during the feeding process to prevent the raw materials from forming large clumps, which would affect the subsequent dissolving time.

[0034] The working principle of this product is as follows: First, connect the external power supply. Then, the raw materials for preparing the cell culture medium are fed into the dissolving tank 1 through the feeding tube 23 on the sealed cover 2. The fixing ring 24 and the cutting blade 25 inside the feeding tube 23 cut the raw materials during the feeding process, preventing large clumps from entering and affecting the subsequent dissolution time. Next, turn on the servo motor 17 to drive the stirring rod 18, the dissolving wall blade 19, and the rotating drum 20 to rotate. The dissolving wall blade 19 and the rotating drum 20 are vertically distributed, stirring the raw materials from above and below. The dispersed raw materials are rotated, allowing them to flow out through the spray holes 21 of the rotating drum 20, thus creating a flowing stream. A measured amount of dissolving liquid is then injected into the steam box 8, and the electric heating tube 11 heats the dissolving liquid through the spacer heat-conducting plate 10, causing it to rise as steam. The high-temperature resistant air pump 13 is then turned on, drawing dissolving steam through the steam ring 15 and spraying it from the multi-hole nozzle 16. Combined with the rotating dissolving wall blade 19 and the rotating drum 20, this accelerates the dissolution of the raw materials inside the dissolving tank 1. During the dissolution process, the raw materials inside the dissolving tank 1 are not stirred and dissolved at a fixed angle. During stirring, the switch of the telescopic cylinder 7 can be opened, allowing the cylinder to extend and retract. This, through the push frame 6 and angle frame 5, tilts the dissolving tank 1 on the adjustment frame 4. This multi-pronged operation ensures more comprehensive dissolution of the raw materials in the cell culture medium preparation device, promoting mixing and reducing dead zones. The assistance of dissolving steam, with its heat and kinetic energy, further accelerates dissolution and improves quality. The stirring process involves both macroscopic agitation and microscopic mixing, increasing dissolution efficiency and reducing shear forces on the cells, thus protecting cell viability. The dissolved raw materials can then be drained from the pipe at the bottom of the dissolving tank 1. After completing the installation and use of the cell culture medium preparation raw material dissolution device according to the above operations, the switches of the telescopic cylinder 7, high-temperature air pump 13, and servo motor 17 should be turned off. If not used for a long period, the external power supply should be disconnected. This completes the usage process of the cell culture medium preparation raw material dissolution device.

[0035] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A cell culture media formulation raw material dissolution apparatus, characterized by: include Dissolving tank (1), the main body used to hold the raw materials; A sealing cap (2) is fixedly installed above the dissolving tank (1) to seal the dissolving space. A rotating shaft (3) is fixedly installed on both sides of the outside of the dissolving tank (1). An adjusting frame (4) is rotatably connected to the outside of the rotating shaft (3). An angle frame (5) is threadedly connected to the outside of the dissolving tank (1). A push frame (6) is fixedly installed at the bottom of the angle frame (5). A telescopic cylinder (7) is fixedly installed on the outside of the adjusting frame (4). A steam box (8) is fixedly installed outside the dissolving tank (1) and has an externally fixed liquid injection port (9). A spacer heat-conducting plate (10) is fixedly installed inside the steam box (8). An electric heating tube (11) is fixedly installed at the bottom of each spacer heat-conducting plate (10). A steam filter screen (12) is fixedly installed inside the steam box (8). A high-temperature resistant air pump (13) is fixedly installed on the top of the steam box (8). A gas extraction pipe (14) is fixedly installed at the output end of the high-temperature resistant air pump (13). A steam ring (15) is fixedly installed at the other end of the gas extraction pipe (14). A multi-hole nozzle (16) is fixedly installed on the outside of the steam ring (15). A servo motor (17) is fixedly installed above the sealing cover (2) for the output end to be fixedly installed with a stirring rod (18), and a dissolving wall blade (19) is fixedly installed on the outside of the stirring rod (18). A flowing drum (20) is fixedly installed at the bottom of the stirring rod (18), and a jet hole (21) is opened on the outside of the flowing drum (20).

2. A cell culture media formulated raw material dissolving apparatus according to claim 1, characterized in that: The front end of the telescopic cylinder (7) is hinged to the bottom of the push frame (6), and the dissolving tank (1) is rotatably connected to the adjusting frame (4).

3. A cell culture media formulation raw material dissolving apparatus according to claim 1, wherein: The steam filter (12) is fixedly disposed above the spacer heat-conducting plate (10), and the steam ring (15) is fixedly installed inside the dissolving tank (1).

4. A cell culture media formulation raw material dissolving apparatus according to claim 1, wherein: The number of the porous nozzles (16) is set to multiple, and the multiple porous nozzles (16) are distributed in a ring array on the steam ring (15).

5. A cell culture media formulation raw material dissolving apparatus according to claim 1, wherein: The dissolving wall blade (19) is rotatably connected to the dissolving tank (1), and the flowing rotating drum (20) and the stirring rod (18) are vertically distributed.

6. A cell culture media formulation raw material dissolving apparatus according to claim 1, wherein: An inner sealing ring (22) is fixedly installed inside the sealing cover (2), and a feeding pipe (23) is fixedly installed above the sealing cover (2). A fixing ring (24) is fixedly installed inside the feeding pipe (23), and a slitting blade (25) is fixedly installed inside the fixing ring (24).

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