PCR detector

Abstract

The utility model discloses a PCR (Polymerase Chain Reaction) detector and belongs to the technical field of biological and medical detection. The device is used for PCR test tube detection, in the device, an upper plate is arranged above a detection module, a bracket in a pressing plate assembly is clamped in a first through hole of the upper plate, a support connected in the bracket is connected with a pressing plate, a second through hole is formed in a supporting platform, and the upper plate comprises a rotating shaft fixing seat and a torsional spring groove; a rotating shaft on the pressing plate is fixed to the upper plate through a rotating shaft fixing base, one end of one torsional spring rotating arm is clamped in the torsional spring groove, and one end of the other torsional spring rotating arm is clamped in the pressing plate. The test tube body enters the test tube groove through the second through hole in the supporting platform, the test tube cover is clamped on the supporting platform, the pressing plate transmits pressing force to the test tube cover through the supporting platform, so that the PCR detection test tube is driven to pop up, the torsional spring provides elastic force for the pressing plate, and the pressing plate is restored to the original position after the PCR detection test tube is taken out. The effect of quickly and simply taking out the PCR detection test tube is achieved.

Description

Technical Field

[0001] This utility model relates to the fields of biology and medical testing technology, and in particular to a PCR testing instrument. Background Technology

[0002] Polymerase Chain Reaction (PCR) is a biological technique that amplifies DNA fragments by artificially controlling external conditions. A PCR instrument is the core equipment for implementing PCR technology. The sample to be tested is placed in a test tube, and then the test tube is placed in the PCR instrument. The presence of the virus is confirmed by amplifying and analyzing the viral nucleic acid.

[0003] Because PCR testing instruments have the characteristics of rapid heating and cooling, the heating pads and heat sinks in the instrument need to be close to the test tubes to achieve this effect. However, when the test tubes are inserted into the instrument, the heating pads and heat sinks are in close contact with the test tubes, making it difficult to remove the test tubes when the test is finished. Utility Model Content

[0004] This invention provides a PCR detection instrument that solves the problem of difficult-to-remove test tubes in related technologies. The technical solution is as follows:

[0005] According to a first aspect of the present invention, a PCR detection instrument is provided for PCR detection test tubes, the PCR detection test tubes including a test tube cap and a test tube body for loading samples, the test tube cap being connected to the test tube body, the test tube cap having a dimension larger than the test tube body in the direction perpendicular to the length of the test tube body, and the PCR detection instrument including: a detection component and a pressure plate component;

[0006] The detection component includes a detection module and an upper plate. The detection module has a test tube groove, and the upper plate is disposed above the test tube groove. The upper plate has a first through hole, and the orthographic projection of the groove opening of the test tube groove on the upper plate is located in the first through hole.

[0007] The pressure plate assembly includes a pressure plate, a bracket, and a torsion spring. The bracket is engaged in the first through hole. The bracket includes a connected support and a support platform. The support is connected to the pressure plate. The support platform has a second through hole adapted to the shape of the test tube body. When the test tube body passes through the second through hole and enters the detection module, the test tube cap is engaged on the support platform.

[0008] The upper plate includes a rotating shaft fixing seat and a torsion spring groove. The pressure plate includes a rotating shaft located on the side of the pressure plate near the upper plate. Both ends of the rotating shaft are connected to the rotating shaft fixing seat. The torsion spring includes an integrally wound torsion spring coil and two torsion spring arms. One end of one torsion spring arm is engaged in the torsion spring groove, and one end of the other torsion spring arm is engaged on the side of the pressure plate away from the upper plate. The torsion spring coil is located between the pressure plate and the upper plate.

[0009] Optionally, the bracket further includes two fixing posts located at the end of the connected bracket away from the support platform;

[0010] The pressure plate has two fixing slots, which are rotatably connected to two fixing columns, and the axis of rotation is parallel to the support platform.

[0011] Optionally, the bracket and the pressure plate are an integral structure.

[0012] Optionally, the detection assembly further includes a housing, which is fitted over the detection module and connected to the upper plate. The housing has a third through hole located directly above the first through hole.

[0013] The pressure plate assembly is located within the space enclosed by the housing and the upper plate.

[0014] Optionally, the housing further includes a sliding cover and a sliding groove, the sliding groove being located inside the housing, the sliding cover cooperating with the sliding groove to move at the third through hole, the sliding groove having a first position and a second position, the sliding cover covering the third through hole when the sliding cover is in the first position, and the third through hole being open when the sliding cover is in the second position.

[0015] Optionally, the housing further includes two latches, and the upper plate further includes two protrusions located at both ends of the upper plate. The housing is engaged with the two protrusions of the upper plate by means of the two latches.

[0016] Optionally, the slide cover also includes a handle located on the side of the slide cover away from the pressure plate assembly.

[0017] Optionally, the pressure plate further includes a pressing part located on the side of the pressure plate away from the upper plate, and the pressing part includes a plurality of anti-slip particles arranged in a circular pattern.

[0018] The beneficial effects of the technical solution provided by this utility model embodiment include at least the following:

[0019] In the PCR detector provided in this embodiment, the detection module has a test tube slot, and an upper plate is positioned above the test tube slot. The upper plate has a first through hole, and the orthographic projection of the test tube slot opening onto the upper plate is located within the first through hole. The bracket of the pressure plate assembly is engaged in the first through hole, and the bracket is connected to the pressure plate via a connected support. The support platform of the bracket has a second through hole adapted to the shape of the test tube body. The pressure plate is connected to a rotating shaft fixing seat located on the upper plate via a rotating shaft. Of the two torsion spring rotating arms, one end of one torsion spring rotating arm is engaged in a torsion spring groove, and one end of the other torsion spring rotating arm is engaged on the side of the pressure plate away from the upper plate. The torsion spring coil is located between the pressure plate and the upper plate. When the PCR test tube is inserted into the PCR detector, the test tube body enters the test tube slot through the second through hole on the support platform, and the test tube cap is engaged on the support platform. When removing the PCR test tube, pressing the pressure plate transmits the pressure through the support platform to the tube cap, causing the PCR test tube to pop out. A torsion spring provides elasticity to the pressure plate, ensuring it returns to its original position after the PCR test tube is removed. This solves the problem of difficult tube removal in related technologies, achieving a quick and easy removal of the PCR test tube. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a schematic diagram of the structure of a PCR detection instrument shown in an embodiment of the present invention;

[0022] Figure 2 for Figure 1 Side view of the PCR testing instrument shown;

[0023] Figure 3 for Figure 1 An exploded view of a portion of the PCR detection instrument shown.

[0024] Figure 4 for Figure 1 A partial structural diagram of the PCR detection instrument shown;

[0025] Figure 5 for Figure 4 A side view of a partial structure of the PCR detection instrument shown;

[0026] Figure 6 for Figure 4 A top view of a portion of the PCR detection instrument shown;

[0027] Figure 7 for Figure 1 A partial structural diagram of another PCR detection instrument is shown;

[0028] Figure 8 for Figure 7 A side view of a partial structure of the PCR detection instrument shown;

[0029] Figure 9 for Figure 7 A side view of a partial structure of the PCR detection instrument shown;

[0030] Figure 10 for Figure 1 The diagram shows a partial structural schematic of the PCR detection instrument.

[0031] Explanation of reference numerals in the attached figures:

[0032] 1-Test tube, 11-Test tube cap, 12-Test tube body;

[0033] 2-Detection component, 21-Detection module, 211-Test tube slot, 22-Upper plate, 221-Rotating shaft fixing seat, 222-Torsion spring groove, 223-First through hole, 23-Housing shell, 231-Third through hole, 232-Sliding cover, 2321-Handle, 233-Sliding groove;

[0034] 3-Pressure plate assembly, 31-Pressure plate, 311-Rotating shaft, 312-Fixing groove, 32-Bracket, 321-Bracket, 322-Supporting platform, 323-Fixing column, 3221-Second through hole, 33-Torsion spring, 331-Torsion spring ring, 332-Torsion spring rotating arm.

[0035] The accompanying drawings have illustrated specific embodiments of the present invention, which will be described in more detail below. These drawings and descriptions are not intended to limit the scope of the present invention in any way, but rather to illustrate the concept of the present invention to those skilled in the art through reference to specific embodiments. Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of this utility model clearer, the embodiments of this utility model will be described in further detail below with reference to the accompanying drawings.

[0037] Figure 1 This is a schematic diagram of the structure of a PCR detection instrument shown in an embodiment of the present invention. Figure 2 for Figure 1 The side view of the PCR detector shown is shown. Figure 3 for Figure 1 The image shows an exploded view of a portion of the PCR detection instrument. Figure 4 for Figure 1 The diagram shows a partial structural representation of the PCR detector, combined with... Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, the PCR instrument is used for PCR test tube 1. The PCR test tube 1 includes a tube cap 11 and a tube body 12 for loading samples. The tube cap 11 is connected to the tube body 12. The dimension of the tube cap 11 in the direction perpendicular to the length of the tube body 12 is larger than that of the tube body 12. The tube body 12 is located in the detection module of the PCR instrument during the detection process. The detection module detects the sample in the tube body 12. After the tube cap 11 is connected to the tube body 12, it can seal the tube body, thereby preventing the sample in the tube body 12 from being contaminated.

[0038] The PCR testing instrument includes: a testing component 2 and a plate pressing component 3;

[0039] The detection component 2 includes a detection module 21 and an upper plate 22. The detection module 21 has a test tube groove 211. The upper plate 22 is disposed above the test tube groove 211. The upper plate 22 has a first through hole 223. The orthographic projection of the groove opening of the test tube groove 211 on the upper plate 22 is located in the first through hole 223.

[0040] The pressure plate assembly 3 includes a pressure plate 31, a bracket 32, and a torsion spring 33. The bracket 32 ​​is snapped into the first through hole 223. The bracket 32 ​​includes a connected support 321 and a support platform 322. The support 321 is connected to the pressure plate 31. The support platform 322 has a second through hole 3221 that is adapted to the shape of the test tube body 12. When the test tube body 12 passes through the second through hole 3221 and enters the detection module 21, the test tube cap 11 is snapped onto the support platform 322.

[0041] The upper plate 22 includes a rotating shaft fixing seat 221 and a torsion spring groove 222. The pressure plate 31 includes a rotating shaft 311, which is located on the side of the pressure plate 31 near the upper plate 22. Both ends of the rotating shaft 311 are connected to the rotating shaft fixing seat 221. The torsion spring 33 includes an integrally wound torsion spring coil 331 and two torsion spring rotating arms 332. One end of one torsion spring rotating arm 332 is engaged in the torsion spring groove 222, and the other end of the torsion spring rotating arm 332 is engaged on the side of the pressure plate 31 away from the upper plate 22. The torsion spring coil 331 is located between the pressure plate 31 and the upper plate 22.

[0042] When using a PCR testing instrument, the PCR test tube 1 passes through the second through-hole 3221, the first through-hole 223, and the slot of the test tube slot 211 into the testing module 21. At this time, the test tube body 12, used to load the sample, is located in the test tube slot 211. The test tube cap 11, which is connected to the test tube body 12, is larger in size in the direction perpendicular to the length of the test tube body 12 and is snapped onto the support platform 322. The test tube body 12 and the test tube cap 11 can be sealed or connected in other ways, which are not limited in this invention. When the PCR test tube 1 is removed after testing, the pressure plate 31 is pressed. The pressure plate 31, through the rotation of the pivot 311, drives the bracket 32 ​​to lift. The support platform 322 applies force to the test tube cap 11, pulling the test tube body 12 out of the test tube slot 211. After the test tube 1 is removed, the pressure plate 31 returns to its initial position under the action of the torsion spring 33, and the pressing point of the pressure plate 31 is parallel to the upper plate 22.

[0043] In summary, the PCR detector provided in this embodiment of the present invention includes a test tube slot on the detection module, an upper plate positioned above the test tube slot, and a first through hole on the upper plate. The orthographic projection of the test tube slot opening onto the upper plate is located within the first through hole. A bracket of the pressure plate assembly is engaged in the first through hole, and the bracket is connected to the pressure plate via a connected support. The support platform of the bracket has a second through hole adapted to the shape of the test tube body. The pressure plate is connected to a rotating shaft fixing seat located on the upper plate via a rotating shaft. Of the two torsion spring arms, one end of one torsion spring arm is engaged in a torsion spring groove, and one end of the other torsion spring arm is engaged on the side of the pressure plate away from the upper plate. The torsion spring coil is located between the pressure plate and the upper plate. When the PCR test tube is inserted into the PCR detector, the test tube body enters the test tube slot through the second through hole on the support platform, and the test tube cap is engaged on the support platform. When removing the PCR test tube, pressing the pressure plate transmits the pressure through the support platform to the tube cap, causing the PCR test tube to pop out. A torsion spring provides elasticity to the pressure plate, ensuring it returns to its original position after the PCR test tube is removed. This solves the problem of difficult tube removal in related technologies, achieving a quick and easy removal of the PCR test tube.

[0044] Figure 5 for Figure 4 The diagram shows a side view of a portion of the PCR detection instrument. Figure 6 for Figure 4 The diagram shows a top view of a portion of the PCR detection instrument. Figure 4 The diagram shown is of the structure when the bracket and pressure plate are not connected. Figure 5 The image shown is a side view after the pressure plate and bracket are connected. Figure 6 The image shown is a top view of the pressure plate and bracket after they are connected. Figure 4 , Figure 5 , Figure 6 As shown:

[0045] The bracket 32 ​​also includes two fixing posts 323, located at the end of the connected support 321 away from the support platform 322. The pressure plate 31 has two fixing grooves 312, which are rotatably connected to the two fixing posts 323, with the axis of rotation parallel to the support platform 322. This rotatable connection between the fixing grooves 312 and the fixing posts 323 allows the bracket 32 ​​and the pressure plate 31 to rotate relative to each other, making the pressure plate assembly more flexible. The support platform 322 has a second through hole 3221, the shape of which matches the shape of the test tube. The fixing posts can be semi-circular, and the fixing grooves are semi-circular grooves adapted to the semi-circular fixing posts. The fixing posts and fixing grooves can also have other shapes; this embodiment of the invention is not limited to these shapes.

[0046] Figure 7 for Figure 1 The diagram shows a partial structure of another PCR detection instrument. Figure 8 for Figure 7 The diagram shows a side view of a portion of the PCR detection instrument. Figure 9 for Figure 7 The diagram shows a side view of a portion of the PCR detection instrument. Figure 7 The diagram shows a structural representation of another connection method between the bracket and the pressure plate. Figure 8 What is shown is Figure 7 The side view showing the connection method. Figure 9 What is shown is Figure 7 A top view showing the connection method. (See attached image.) Figure 7 , Figure 8 , Figure 9 As shown, the bracket 32 ​​and the pressure plate 31 are an integral structure. The bracket 32 ​​and the pressure plate 31 can be manufactured using a one-piece molding process, eliminating the need to manufacture connecting parts between the bracket and the pressure plate, simplifying the structure of the pressure plate assembly. Furthermore, the integral structure of the pressure plate and bracket eliminates concerns about loosening at the connection point during repeated test tube removal, thus reducing the probability of maintenance of the pressure plate assembly. Other connection methods can also be used for the bracket and pressure plate in the pressure plate assembly; this embodiment of the invention does not limit the specific connection methods described herein.

[0047] Figure 10 for Figure 1 The diagram shows a partial structural schematic of the PCR detection instrument. Figure 10 A structural diagram of a shell is shown, combined with Figure 1 and Figure 10As shown, the detection assembly also includes a housing 23, which is fitted over the detection module 21 and connected to the upper plate 22. The housing has a third through hole 231, located directly above the first through hole 223. The pressure plate assembly is located within the space enclosed by the housing 23 and the upper plate 22. The detection module may include a thermal cycling system, an optical detection system, a control system, and a light source system. The light source system provides excitation light for fluorescence detection. The optical detection system collects and analyzes the fluorescence signal emitted by the sample, converts the light signal into an electrical signal, and transmits it to a computer for data processing and analysis. The thermal cycling system is used to precisely control the reaction temperature and time. The control system coordinates the operation of each component of the detection module and collects, processes, and analyzes the detection data. The detection module may also include other systems, which are not limited in this embodiment.

[0048] Optionally, the housing 23 also includes a sliding cover 232 and a sliding groove 233. The sliding groove 233 is located inside the housing 23. The sliding cover 232 and the sliding groove 233 cooperate to move at the third through hole 231. The sliding groove 233 has a first position and a second position. When the sliding cover 232 is in the first position, the sliding cover 232 covers the third through hole 231. When the sliding cover 232 is in the second position, the third through hole 231 is open.

[0049] Optionally, the sliding cover 232 also includes a handle 2321, which is located on the side of the sliding cover 232 away from the pressure plate assembly. By sliding the handle, the sliding cover 232 moves back and forth at the third through hole. When a test tube needs to be inserted, the sliding cover 232 is moved to the second position of the slide groove; when performing testing or not using PCR testing, the sliding cover 232 is moved to the first position of the slide groove. The sliding cover covers the first through hole, the second through hole, the third through hole 231, and the opening of the test tube groove, thereby preventing dust and other contaminants from entering the test tube groove through the aforementioned through holes. In addition, the side of the sliding cover 232 away from the pressure plate assembly may also include a protrusion or other components that facilitate pushing the sliding cover; this embodiment of the present invention is not limited thereto.

[0050] like Figure 3 As shown, the housing 23 also includes two latches 234. Figure 3 (Only one buckle is shown in the image). The upper plate 22 also includes two protrusions 223, located at both ends of the upper plate 22. The housing 23 is engaged with the two protrusions 223 of the upper plate by two buckles 234. The housing can be divided into a main housing, a front cover, and a rear cover. The main housing is a housing with a sliding cover and a sliding groove. The front cover and the rear cover are each provided with two buckles, which are engaged with the two protrusions of the upper plate. Figure 10 As shown, Figure 10 The diagram shows the main housing 234 and the front cover 235, with the rear cover located on the other side of the main housing. Other connection methods, such as snap-fit ​​connections, can also be used between the housing and the upper plate; this embodiment of the invention is not limited to these methods.

[0051] like Figure 4 As shown, the pressure plate 31 also includes a pressing part 313, which is located on the side of the pressure plate 31 away from the upper plate. The pressing part 313 includes multiple anti-slip particles 3131 arranged in a circular pattern. By setting multiple anti-slip and anti-wear particles 3131, the slippage and difficulty in applying force during the process of removing the test tube by pressing the pressure plate can be avoided, thereby further improving the convenience of removing the PCR test tube. In addition, the multiple anti-slip particles can also be arranged in a square, trapezoidal or other shapes, which is not limited to this embodiment of the present invention.

[0052] like Figure 3 As shown, the torsion spring 33 is made of a single elastic material. One torsion spring arm 332 on one side of the torsion spring coil is wound into a door-frame structure, which engages with a torsion spring groove on the upper plate, fixing one end of the torsion spring in the upper plate. The other torsion spring arm 332 on the other side of the torsion spring coil is wound into two parallel lines, the ends of which are engaged inside the pressure plate 31. The specific connection method is not limited in this embodiment. The two installed torsion spring arms 332 are set at an acute angle. When the pressure plate is pressed, the torsion spring undergoes elastic deformation and stores deformation energy. After pressing, the torsion spring, under the action of the stored deformation energy, drives the pressure plate back to its initial position.

[0053] In this invention, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. The term "multiple" refers to two or more unless otherwise expressly defined.

[0054] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A PCR detector, characterized by, For PCR detection test tubes, the PCR detection test tube includes a test tube cap and a test tube body for loading samples, the test tube cap is connected to the test tube body, the dimension of the test tube cap in the direction perpendicular to the length of the test tube body is larger than that of the test tube body, the PCR detection instrument includes: a detection component and a pressure plate component; The detection component includes a detection module and an upper plate. The detection module has a test tube groove, and the upper plate is disposed above the test tube groove. The upper plate has a first through hole, and the orthographic projection of the groove opening of the test tube groove on the upper plate is located in the first through hole. The pressure plate assembly includes a pressure plate, a bracket, and a torsion spring. The bracket is engaged in the first through hole. The bracket includes a connected support and a support platform. The support is connected to the pressure plate. The support platform has a second through hole adapted to the shape of the test tube body. When the test tube body passes through the second through hole and enters the detection module, the test tube cap is engaged on the support platform. The upper plate includes a rotating shaft fixing seat and a torsion spring groove. The pressure plate includes a rotating shaft located on the side of the pressure plate near the upper plate. Both ends of the rotating shaft are connected to the rotating shaft fixing seat. The torsion spring includes an integrally wound torsion spring coil and two torsion spring arms. One end of one torsion spring arm is engaged in the torsion spring groove, and one end of the other torsion spring arm is engaged on the side of the pressure plate away from the upper plate. The torsion spring coil is located between the pressure plate and the upper plate.

2. The PCR detector of claim 1, wherein, The bracket also includes two fixing posts, which are located at the end of the connected bracket away from the support platform; The pressure plate has two fixing slots, which are rotatably connected to two fixing columns, and the axis of rotation is parallel to the support platform.

3. The PCR detector of claim 1, wherein, The bracket and the pressure plate are an integral structure.

4. The PCR detector of claim 1, wherein, The detection component also includes a housing, which is fitted over the detection module and connected to the upper plate. The housing has a third through hole, which is located directly above the first through hole. The pressure plate assembly is located within the space enclosed by the housing and the upper plate.

5. The PCR detector of claim 4, wherein, The housing also includes a sliding cover and a sliding groove. The sliding groove is located inside the housing. The sliding cover and the sliding groove cooperate to move at the third through hole. The sliding groove has a first position and a second position. When the sliding cover is in the first position, the sliding cover covers the third through hole. When the sliding cover is in the second position, the third through hole is open.

6. The PCR detector of claim 4, wherein, The housing also includes two buckles, and the upper plate also includes two protrusions located at both ends of the upper plate. The housing is engaged with the two protrusions of the upper plate by means of the two buckles.

7. The PCR detector of claim 5, wherein, The sliding cover also includes a handle located on the side of the sliding cover away from the pressure plate assembly.

8. The PCR detector of claim 1, wherein, The pressure plate also includes a pressing part, which is located on the side of the pressure plate away from the upper plate. The pressing part includes multiple anti-slip particles arranged in a circular pattern.

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