Portable biological experiment detection box

Abstract

The utility model provides a portable biological experiment detection box which comprises a box body, a first reaction tank, a second reaction tank and a detection tank are arranged in the box body, the first reaction tank, the second reaction tank and the detection tank are all provided with air vents which are communicated with the interiors of the first reaction tank, the second reaction tank and the detection tank and can be closed, and the detection tank is provided with electrode insertion holes into which electrodes can be inserted; inlets of the first reaction tank, the second reaction tank and the detection tank are connected with sample adding pipelines with sample adding ports, valves for controlling connection and disconnection are arranged on the sample adding pipelines and the connecting pipelines, and the sample adding pipelines are further connected in series with an air inlet connected with a pneumatic driving mechanism. Positive-pressure airflow is injected from different air inlets through the pneumatic driving mechanism, and liquid is pushed to flow into the first reaction tank, the second reaction tank and the detection tank by controlling opening and closing of valves on the sample adding pipeline and the connecting pipeline, so that part of solution is prevented from being remained in the sample adding pipeline, the connecting pipeline, the first reaction tank and the second reaction tank; the detection precision is improved.

Description

Technical Field

[0001] This utility model belongs to the field of medical technology, specifically relating to a portable biological experimental detection kit. Background Technology

[0002] Early diagnosis of prostate cancer relies on the discovery and precise detection of highly specific biomarkers. α-Methylacyl-CoA racemic enzyme (AMACR), a metabolic enzyme that is significantly overexpressed in prostate cancer tissues, has an expression level closely related to tumor progression and has become a more specific molecular marker than traditional PSA.

[0003] The CRISPR system was initially discovered as an adaptive immune mechanism in bacteria, with its core components including Cas proteins (such as Cas9, Cas12, and Cas13) and guide RNA (gRNA). In recent years, the CRISPR-Cas system has been redesigned as a molecular diagnostic tool, overcoming the limitations of traditional nucleic acid detection technologies and providing a revolutionary solution for developing next-generation AMACR detection tools.

[0004] A MIA (Multiplexed Immunosensor Array) electrochemical analyzer is a multi-channel electrochemical biosensor platform that uses probes (such as antibodies or nucleic acids) immobilized on electrode surfaces to capture target molecules and detect changes in current, voltage, or impedance caused by binding events. Detecting the novel prostate cancer biomarker AMACR using an MIA electrochemical analyzer involves a complex detection process, requires specialized laboratory personnel, and has a high technical threshold. Utility Model Content

[0005] To address the technical problems existing in the prior art, this utility model provides a portable biological experimental detection kit.

[0006] In this embodiment of the present invention, a portable biological experimental detection kit includes a box body in which a first reaction chamber, a second reaction chamber, and a detection chamber are installed. The outlet of the first reaction chamber is connected to the inlet of the second reaction chamber, and the outlet of the second reaction chamber is connected to the inlet of the detection chamber via connecting pipes. The first reaction chamber, the second reaction chamber, and the detection chamber each have a vent that can be sealed and communicates with their interiors. The detection chamber is provided with an electrode insertion hole for inserting an electrode into it. The inlets of the first reaction chamber, the second reaction chamber, and the detection chamber are all connected to a sample dispensing pipe with a sample dispensing port. The sample dispensing pipe and the connecting pipe are provided with valves for controlling the on / off state. The sample dispensing pipe is also connected in series with an air inlet located upstream for connection to a pneumatic drive mechanism.

[0007] Compared with the prior art, the beneficial effects of the superior technical solution of this utility model include:

[0008] 1) The detection kit of this utility model integrates the CRISPR-Cas12a non-nucleic acid target detection process. Compared with the detection of AMACR, a novel biomarker for prostate cancer, by using the MIA electrochemical analyzer, this solution simplifies the complex detection process. Users can add the corresponding detection reagents according to the detection steps and complete the detection with the assistance of the electrochemiluminescence detector without the need for professional training.

[0009] 2) This utility model breaks through the dependence on professional equipment, and the operator does not need to receive professional training. This convenient design significantly lowers the threshold of molecular diagnostic technology and provides an innovative solution for home health monitoring.

[0010] 3) The detection box is equipped with an air inlet connected to the sample dispensing pipe. A pneumatic drive mechanism injects positive pressure airflow from different air inlets. At the same time, by controlling the opening and closing of valves on the sample dispensing pipe and the connecting pipe, the liquid is pushed into the first reaction chamber, the second reaction chamber and the detection chamber, so as to avoid residual solution in the sample dispensing pipe, the connecting pipe, the first reaction chamber and the second reaction chamber, and improve the detection accuracy.

[0011] 4) Staged pneumatic actuation can accommodate diverse detection schemes. For example, in a small number of experimental research designs, the actual order of adding reagents may need to be fine-tuned, and valves and pneumatic actuation can accommodate more complex detection processes. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the structure of a CRISPR-Cas12a non-nucleic acid substance detection kit according to an embodiment.

[0013] Figure 2 This is a schematic diagram of the valve in one embodiment, with the valve in the open state.

[0014] The reference numerals in the accompanying drawings of the instruction manual include: 1. Box body; 2. First reaction cell; 3. Second reaction cell; 4. Detection cell; 5. First connecting pipe; 6. Second connecting pipe; 7. Vent hole; 8. Electrode insertion hole; 9. First sample addition pipe; 10. Second sample addition pipe; 11. Third sample addition pipe; 12. Fourth sample addition pipe; 13. First sample addition port; 14. Second sample addition port; 15. Fourth sample addition port; 16. First valve; 17. Second valve; 18. Third valve; 19. Fourth valve; 20. Fifth valve; 21. First air inlet; 22. Second air inlet; 23. Third air inlet; 24. Fourth air inlet; 25. Sixth valve; 26. Air tube; 27. Flexible hose; 28. Sliding rod; 29. ​​Buckle; 30. Button; 31. Valve core; 32. Spring; 33. Support column; 34. Detailed Implementation

[0015] The embodiments of this utility model are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0016] This embodiment provides a portable biological experimental detection kit, such as Figure 1 As shown, in a preferred embodiment, the detection box includes a box body 1, in which a first reaction tank 2, a second reaction tank 3, and a detection tank 4 are installed sequentially from left to right. The outlet of the first reaction tank 2 is connected to the inlet of the second reaction tank 3, and the outlet of the second reaction tank 3 is connected to the inlet of the detection tank 4 via connecting pipes. The first reaction tank 2, the second reaction tank 3, and the detection tank 4 each have a vent 7 that can be sealed and communicates with their interiors. The vent 7 is normally open. The detection tank 4 is provided with an electrode insertion hole 8 for inserting an electrode into it. The vent 7 of the first reaction tank 2 and the second reaction tank 3 are located at the top, and the electrode insertion hole 8 is located at the top of the detection tank 4, occupying the top position. The vent 7 of the detection tank 4 can be located on its side wall.

[0017] The inlets of the first reaction tank 2, the second reaction tank 3, and the detection tank 4 are all connected to sample dispensing pipes with sample dispensing ports. Valves for controlling the on / off state are installed on the sample dispensing pipes and connecting pipes. An air inlet located upstream of the sample dispensing pipe is also connected in series for connection to a pneumatic drive mechanism. The sample dispensing port and air inlet are exposed outside the housing 1. The valve operating buttons are located outside the housing 1; for example, the sample dispensing port is located at the top of the housing 1, and the air inlet extends from the side wall of the housing 1 outside the housing 1. The housing 1 encapsulates the first reaction tank 2, the second reaction tank 3, the detection tank 4, the sample dispensing pipes, and the connecting pipes, making it easy to carry.

[0018] Specifically, the sample dispensing conduit includes a first sample dispensing conduit 9 with a first sample dispensing port 13 and a second sample dispensing conduit 10 with a second sample dispensing port 14, connected in parallel to the first reaction cell 2; a third sample dispensing conduit 11 with a third sample dispensing port 15, connected to the second reaction cell 3; and a fourth sample dispensing conduit 12 with a fourth sample dispensing port 16, connected to the detection cell 4. The four sample dispensing ports are respectively connected to the outlets of four pipettes, allowing for sample dispensing via pipettes or manually via syringe. Preferably, the sample dispensing ports are all upright, funnel-shaped, and connected to the sample dispensing conduit. The sample dispensing conduit is connected to the upper part or top of the first reaction cell 2, the second reaction cell 3, and the detection cell 4, above the liquid level within them, to facilitate the propulsion of liquid by a positive pressure airflow.

[0019] The valves include a first valve 17 on the first sample addition pipe 9, a sixth valve 26 on the second sample addition pipe 10, a fifth valve 21 on the third sample addition pipe 11, a fourth valve 20 on the fourth sample addition pipe 12, a second valve 18 on the first connecting pipe 5 connecting the first reaction tank 2 and the second reaction tank 3, and a third valve 19 on the second connecting pipe 6 connecting the second reaction tank 3 and the detection tank 4.

[0020] The air inlets include a first air inlet 22 connected to the first sample dispensing pipe 9, a second air inlet 23 connected to the second sample dispensing pipe 10, a third air inlet 24 connected to the third sample dispensing pipe 11, and a fourth air inlet 25 connected to the fourth sample dispensing pipe 12. The air inlets are connected to a pneumatic drive mechanism, which can be a micro-pump or a syringe. Positive pressure airflow is injected through the pneumatic drive mechanism to drive the liquid flow. Preferably, the air inlets are connected to the sample dispensing pipes via an air pipe 27, which is a tapered tube with an inlet end larger than an outlet end to maintain a pressure difference between the two ends and prevent leakage.

[0021] When using this test kit for biological experiments, such as using the CRISPR-Cas12a non-nucleic acid target to detect the novel biomarker AMACR for prostate cancer.

[0022] Open the first valve 17, and close the second valve 18, third valve 19, fourth valve 20, fifth valve 21, and sixth valve 26. Add human serum containing AMACR through the first sample application port 13. After the liquid is added, most of the liquid will flow rapidly into the first reaction chamber 2 through capillary action. Due to the seal, air cannot escape, causing droplet obstruction. This includes opening the first valve 17 and closing the other valves, while keeping the vent 7 of the first reaction chamber 2 open. The positive pressure airflow generated by the pneumatic drive mechanism enters through the first air inlet 22, pushing the residual liquid in the first sample application tube 9 into the first reaction chamber 2. It should be noted that all sample application ports have a T-shaped structure to increase resistance. When air enters through the first air inlet 22, the resistance at the sample application port is high, and the residual liquid in the first sample application tube 9 can only enter the first reaction chamber 2. The subsequent process is the same.

[0023] Using the same method, a series of probes containing SNAs are added to the first reaction cell 2 through the second sample loading port 14. The sixth valve 26 is opened and the other valves are closed, so that the vent 7 on the first reaction cell 2 is opened. The positive pressure airflow generated by the pneumatic drive mechanism enters from the second air inlet 23, pushing the remaining probes in the second sample loading pipe 10 into the first reaction cell 2. After the probe is adapted to AMACR, it dissociates and releases a series of activators. After a period of reaction, the solution containing DNA activators is collected using the existing magnetic separation method (because the solution contains an enzyme of about 200 nm, which can be loaded onto a magnet, such as onto a ferromagnetic material, iron(III) oxide, so it can be separated by magnetic adsorption + semi-permeable membrane filtration. The specific separation using a semi-permeable membrane + magnet is an existing technology and will not be detailed here). The second valve 18 is opened and the other valves are closed. The vent 7 of the first reaction pool 2 is blocked with a finger, and the vent 7 of the second reaction pool 3 is opened. The positive pressure airflow generated by the pneumatic drive mechanism enters from the second air inlet 23, pushing the mixture (containing activators, etc.) in the first reaction pool 2 into the second reaction pool 3.

[0024] After the liquid in the first reaction chamber 2 has been completely transferred to the second reaction chamber 3, CRISPR-Cas12a reaction solution is added through the third sample loading port 15 in the same manner. The following reaction mixture (1 μM of Cas12a (cpf1), 0.9 μL of crRNA (1 μM), 3 μL of NEB buffer, and 5.2 μL of RNase-Free) is added to the second reaction chamber 3 for further reaction. The fifth valve 21 is opened, and the other valves are closed, opening the vent 7 on the first reaction chamber 2. A positive pressure gas flow generated by the pneumatic drive mechanism enters through the third air inlet 24, pushing the residual liquid in the third sample loading tube 11 into the second reaction chamber 3. It should be noted that the CRISPR-Cas12a reaction solution can also be replaced with pre-prepared CRISPR-Cas12a lyophilized solution. This lyophilized solution is prepared using a vacuum low-temperature freeze dryer. The activity of the lyophilized solution is generally restored by adding water, and the enzyme activity is maintained by trehalose within the lyophilized solution.

[0025] Following the same procedure, open the fifth valve 21 and the third valve 19, and close the other valves to close the vent 7 of the second reaction tank 3 and open the vent 7 of the detection tank 4. The positive pressure airflow generated by the pneumatic drive mechanism enters through the third air inlet 24, pushing the mixture in the second reaction tank 3 into the detection tank 4. Then close the fifth valve 21 and the third valve 19. After the mixture has incubated in the detection tank 4 for two hours, add S2O8 through the fourth sample addition port 16 in the same manner. 2-Add buffer solution to detection cell 4, open fourth valve 20, close other valves, so that vent 7 on detection cell 4 is open, and positive pressure airflow generated by pneumatic drive mechanism enters from fourth air inlet 25, removing residual S2O8 in fourth sample addition pipeline 12. 2- The buffer solution is pushed into the detection cell 4. The modified electrode (e.g., a glassy carbon electrode modified with AuAgNCs-MOF-5) is inserted into the electrode insertion hole 8 above the detection cell 4 and immersed in the detection solution in the detection cell 4. The modified electrode is connected to an electrochemiluminescence detector (specifically, an MPI-E ECL analysis system, such as that from Anruimai Analytical Instruments Co., Ltd.), and the electrochemiluminescence detector detects the detection solution on the modified electrode.

[0026] The detection kit of this invention can be combined with an MIA analyzer or an electrochemical sensing analysis system to realize the process of data acquisition, sample addition, automatic analysis and diagnosis, which simplifies the complex detection process and eliminates the need for professional training; moreover, based on this detection kit, automated control programs can be designed and applied.

[0027] In this invention, the inner surfaces of the sample addition pipe and the connecting pipe are made of hydrophobic material to prevent the detection liquid and reaction liquid from being left in the pipe.

[0028] In another preferred embodiment, the first reaction tank 2, the second reaction tank 3, and the detection tank 4 are made of quartz glass and are detachably connected to the housing 1 (e.g., by snap-fit; specifically, the housing 1 may have a retaining edge for securing the first reaction tank 2, the second reaction tank 3, and the detection tank 4). The lids of the first reaction tank 2, the second reaction tank 3, and the detection tank 4 are also detachable for easy disassembly and cleaning. Furthermore, the detachable structure allows the detection box to be recycled and reused, significantly reducing detection and development costs.

[0029] More preferably, the first connecting pipe 5 connecting the first reaction tank 2 and the second reaction tank 3 is inclined, and the first connecting pipe 5 slopes upward from the bottom of the first reaction tank 2 to connect to the top of the second reaction tank 3. This is to ensure that all the liquid in the first reaction tank 2 flows into the second reaction tank 3 to avoid leakage and measurement deviation, and also to ensure that the liquid in the second reaction tank 3 does not flow back into the first reaction tank 2. Similarly, the second connecting pipe 6 connecting the second reaction tank 3 and the detection tank 4 is also inclined, and the second connecting pipe 6 slopes upward from the bottom of the second reaction tank 3 to connect to the top of the detection tank 4. This is to ensure that all the liquid in the second reaction tank 3 flows into the detection tank 4 to avoid leakage and measurement deviation, and also to ensure that the liquid in the detection tank 4 does not flow back into the second reaction tank 3. Preferably, the inclination angle of the first connecting pipe 5 and the second connecting pipe 6 is 25° to 30°, which maintains the liquid surface seal while preventing liquid from prematurely entering the next reaction chamber (including the second reaction tank 3 and the detection tank 4).

[0030] More preferably, the box body 1 is equipped with a temperature control device, or the box body 1 is a constant temperature box that can maintain a constant temperature of 37°C (the fixed temperature of the CRISPR system), which meets the needs of nursing points and the feasibility of popularization and promotion. Specifically, a partition can be added to the bottom of the box body 1 to set up a chamber, and a sliding drawer can be connected in the chamber. The drawer can be used to place a small temperature control device. When collecting the solution containing DNA activators by magnetic separation, the enzyme-loaded magnet (permanent magnet) is also placed in the drawer.

[0031] More preferably, the upper end of the housing 1 is open, and a detection cover (not shown in the figure) is movably connected to the housing 1 to close the upper opening. The detection cover is connected to the housing 1 by a hinge or latch, and is openable and equipped with a locking structure, similar to the connection method of a door and a door frame. The primary function of the detection cover is to maintain the temperature of the reaction system (insulation material), to prevent liquid leakage due to tilting, and to facilitate the disassembly and cleaning of the first reaction tank 2, the second reaction tank 3, and the detection tank 4 components.

[0032] In this embodiment, the test cover is provided with four through holes that are respectively aligned with the four sample inlets. The size of the through holes is larger than the size of the sample inlets, so that sample addition is not affected when the test box is used with the test cover closed.

[0033] In this invention, the valve structure can adopt existing medical valves capable of controlling the on / off state of pipelines. Preferably, the portion of the sample delivery pipeline and connecting pipeline connected to the valve uses an elastic flexible tube (such as a rubber flexible tube), while the remaining portion uses a plastic tube. The valve structure can adopt existing valves capable of locking onto the elastic flexible tube to control its on / off state. The elastic flexible tube and plastic tube are an integral structure (e.g., they are heat-fused or glued together, which is conventional technology and will not be detailed here), which can be completed during manufacturer processing, eliminating the need for reconnection by testing personnel and avoiding leakage and length discrepancies during assembly. During assembly, only the plastic tube needs to be connected to the port of the reaction chamber (including the first reaction tank 2, the second reaction tank 3, and the detection tank 4), without needing to connect the elastic flexible tube, reducing the difficulty of connection. During disassembly, the valve is in the open state, releasing the restriction on the elastic flexible tube, facilitating the removal and insertion of the elastic flexible tube from the valve.

[0034] In one implementation, the valve may employ the pin structure of an automatic pen, such as... Figure 2As shown, the valve includes a housing, a sliding rod 29 installed in the housing and slidably connected to the inner wall of the housing, a buckle 30 connected to the upper end of the sliding rod 29, a button 31 connected to the top of the buckle 30 and extending out of the housing, a valve core 32 that abuts against the lower end of the sliding rod 29 and can slide in the housing, a spring 33 sleeved on the sliding rod 29, and a support column 34 fixed in the housing. The valve core 32 is an elastic ball, and an elastic hose 28 is located between the support column 34 and the valve core 32.

[0035] Under normal conditions, the valve core 32 is away from the support column 34, the valve core 32 does not compress the elastic hose 28, and there is a gap between the elastic hose 28 and the support column 34, so the valve is in the open state. Pressing the top button 31 causes the sliding rod 29 to push the valve core 32 closer to the support column 34. The valve core 32 presses against the elastic hose 28 against the support column 34, closing the valve and preventing the elastic hose 28 from conducting. Because it is an elastic hose 28, releasing the button 31 and the valve core 32 rebounding also ensures that the elastic hose 28 is not conducting. Therefore, most of the cross-section can be kept in a closed state, and the surface tension of the droplet will prevent it from passing through the small gap on its own, thus achieving the valve closing function. Pressing the top button 31 again moves the valve core 32 away from the support column 34, opening the valve, and the elastic hose 28 returns to its original deformation and conducts. The valve in this embodiment adopts the pin structure of an automatic pen, and its specific structure and working principle are existing technologies and will not be described in detail here.

[0036] It should be noted that the valve can also adopt other structures, such as a worm gear valve structure, as disclosed in CN114060540A. This valve uses a rotating worm to rotate two sets of worm wheels, which in turn drive two semi-rings (equivalent to valve cores) to rotate, thus opening and closing the valve. In practical applications, the two semi-rings are positioned on both sides of the flexible hose. The worm and worm wheels are mounted on a bracket fixed in the housing and supported by the bracket. Rotating the worm causes the two sets of worm wheels to rotate, which in turn drives the two semi-rings to rotate away from or towards the flexible hose. Rotating the worm moves the two semi-rings away from the flexible hose, opening the valve and allowing the flexible hose to conduct; rotating the worm in the opposite direction moves the two semi-rings towards the flexible hose and compresses it, closing the valve and de-conducting the flexible hose.

[0037] In practice, the worm gear valve can also be replaced with a rack and pinion valve. In this case, the rack moves linearly, causing the two sets of gears to rotate and drive the valve core to rotate, thus opening and closing the valve. This is also existing technology and will not be described in detail here.

[0038] Preferably, when the valve is closed by rotating the worm gear / sliding rack, the worm gear / rack should also be locked to prevent the elastic hose from rebounding and leaking. Specifically, a buckle can be provided on the housing. When the valve is closed, the buckle engages and locks the top surface of the worm gear / rack, or a snap-fit ​​part can be provided on the worm gear / rack to engage with the buckle to achieve locking. These are all existing technologies and will not be described in detail here.

[0039] Although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A portable biological experimental detection kit, characterized in that, The device includes a housing, in which a first reaction tank, a second reaction tank, and a detection tank are installed. The outlet of the first reaction tank is connected to the inlet of the second reaction tank, and the outlet of the second reaction tank is connected to the inlet of the detection tank, all through connecting pipes. The first reaction tank, the second reaction tank, and the detection tank each have a vent that can be sealed and communicates with their interiors. The detection tank is provided with an electrode insertion hole for inserting an electrode into it. The inlets of the first reaction tank, the second reaction tank, and the detection tank are all connected to a sample addition pipe with a sample addition port. The sample addition pipe and the connecting pipe are equipped with valves for controlling the on / off state. The sample addition pipe is also connected in series with an air inlet located upstream of it for connection to a pneumatic drive mechanism.

2. The portable biological experimental detection kit according to claim 1, characterized in that, The sampling pipeline includes a first sampling pipeline with a first sampling port and a second sampling pipeline with a second sampling port, which are connected in parallel with the first reaction cell; a third sampling pipeline with a third sampling port, which is connected to the second reaction cell; and a fourth sampling pipeline with a fourth sampling port, which is connected to the detection cell. The valves include a first valve on the first sample addition pipeline, a sixth valve on the second sample addition pipeline, a fifth valve on the third sample addition pipeline, a fourth valve on the fourth sample addition pipeline, a second valve on the first connecting pipeline connecting the first reaction tank and the second reaction tank, and a third valve on the second connecting pipeline connecting the second reaction tank and the detection tank. The air inlet includes a first air inlet connected to the first sample dispensing pipe, a second air inlet connected to the second sample dispensing pipe, a third air inlet connected to the third sample dispensing pipe, and a fourth air inlet connected to the fourth sample dispensing pipe.

3. The portable biological experimental detection kit according to claim 1, characterized in that, The sample application port is connected to the outlet of the pipette, and the sample is applied through the pipette. Alternatively, the sample can be manually added to the injection port using a syringe.

4. A portable biological experimental detection kit according to claim 1, characterized in that, The sample inlet is an upright, funnel-shaped inlet that connects to the sample dispensing pipe.

5. A portable biological experimental detection kit according to claim 1, characterized in that, The air inlet is connected to the sample addition pipeline via a gas pipe, which is a tapered pipe with the inlet end larger than the outlet end.

6. A portable biological experimental detection kit according to claim 1, characterized in that, The first connecting pipe connecting the first reaction tank and the second reaction tank is inclined and connects to the upper part of the second reaction tank from the bottom of the first reaction tank upward. The second connecting pipe connecting the second reaction tank and the detection tank is inclined, and the second connecting pipe is inclined upward from the bottom of the second reaction tank to connect to the upper part of the detection tank.

7. A portable biological experimental detection kit according to claim 1, characterized in that, The first reaction cell, the second reaction cell, and the detection cell are made of quartz glass and are detachably connected to the housing.

8. A portable biological experimental detection kit according to any one of claims 1-7, characterized in that, The box is equipped with a temperature control device, or the box is a constant temperature box.

9. A portable biological experimental detection kit according to any one of claims 1-7, characterized in that, The bottom of the box is slidably connected to a pull-out storage drawer.

10. A portable biological experimental detection kit according to any one of claims 1-7, characterized in that, The box has an opening at the top, and a detection cover that can close the opening at the top is movably connected to the box.

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