Cell culture bottle and feeding mechanism

Abstract

The utility model discloses a cell culture bottle and a feeding mechanism, and relates to the technical field of cell culture. A first bottle cap is installed on a bottle opening of the cell culture bottle in a threaded mode, feeding pipes are arranged on the left side and the right side of the culture bottle, second bottle caps are installed on the feeding pipes in a threaded mode, and filtering membranes are arranged in the feeding pipes. The feeding mechanism comprises a bottom plate, a material box fixedly installed at the top of the bottom plate, a stirring mechanism arranged on the material box and a guide pipe capable of being communicated with the culture bottle, and a placing frame is fixedly installed on the bottom plate and used for fixing the culture bottle. A supplemented culture medium in a material box enters a culture bottle through a guide pipe and is filtered by a filtering membrane, so that pollution to cells can be prevented, and a stirring mechanism in a feeding mechanism can quickly turn over the supplemented culture medium at the bottom to the top through rotation, so that the supplemented culture medium can be uniformly mixed, and the feeding efficiency is improved. In addition, an ultraviolet lamp strip can better disinfect the fed culture medium, and the practicability of the device is improved.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a cell culture flask and a feeding mechanism. Background Technology

[0002] Cell culture flasks play a crucial role in cell culture, providing a stable, safe, and efficient environment for cell growth and proliferation. In research fields such as biology, medicine, and biotechnology, cell culture flasks are indispensable experimental tools. Modern cell culture flasks consist of two parts: the cap and the body. One cell culture method is fed-batch culture, which requires long-term incubation periods of 14 to 16 days. This involves multiple additions of culture medium during the process. If cell contamination occurs during these steps, the experiment will fail, significantly increasing both time and financial costs and resulting in substantial losses.

[0003] To prevent cell culture medium from contaminating cells, existing cell culture flasks typically filter and sterilize the culture medium before adding it. Ultraviolet (UV) sterilization, due to its high stability, is often used to sterilize this medium. While this helps prevent cell contamination, it doesn't consider that the culture medium is usually a nutrient solution for cells, typically transparent or pale yellow. Although the high transparency of UV light slightly enhances its penetration, the effective penetration depth is only about 1-2 cm. Furthermore, if the culture medium contains amino acids, vitamins, or organic acids, these substances may absorb UVC energy, reducing the actual dose of UV light reaching deeper layers. This means the culture medium at the bottom cannot be properly sterilized, thus reducing the sterilization effect of the device.

[0004] Therefore, a cell culture flask and feeding mechanism are proposed. Utility Model Content

[0005] The purpose of this invention is to provide a cell culture flask and feeding mechanism that can solve the problem that some devices cannot effectively sterilize the feeding culture medium and that cell contamination is easily caused when feeding the culture medium, while also ensuring the uniformity of mixing during feeding.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a cell culture flask and a feeding mechanism, wherein a first cap is threaded onto the mouth of the culture flask, and feed pipes are provided on both the left and right sides of the culture flask. A second cap is threaded onto the feed pipe, and a filter membrane is provided inside the feed pipe. The feeding mechanism includes a base plate and a material box fixedly installed on the top of the base plate, as well as a stirring mechanism and a conduit that can communicate with the culture flask on the material box. A placement rack is fixedly installed on the base plate for fixing the culture flask.

[0007] Preferably, the agitation mechanism includes a motor fixedly installed on the front side of the material box, and also includes a mounting frame, an agitator wheel, a baffle, a transmission box, a first transmission gear, and a second transmission gear. A set of mounting frames is rotatably installed on the inner walls of both the front and rear sides of the material box, and an agitator wheel is rotatably installed between the mounting frames. The output end of the motor extends into the interior of the material box and is fixedly connected to the mounting frame. A transmission box is fixedly installed on the rear side of the material box, and a through groove is provided on the rear side of the material box. The interior of the material box communicates with the interior of the transmission box through the through groove. A baffle is rotatably installed on the inner wall of the front side of the transmission box. One end of the agitator wheel extends through the through groove through the baffle and extends into the interior of the transmission box, where a second transmission gear is fixedly installed. A first transmission gear is fixedly installed on the inner wall of the rear side of the transmission box, and one end of the first transmission gear is rotatably connected to the baffle. The first transmission gear meshes with the second transmission gear.

[0008] Preferably, multiple sets of ultraviolet lamp strips are fixedly installed on the inner top of the material box and are distributed in a front-to-back manner.

[0009] Preferably, a liquid inlet pipe is fixedly installed on the left side of the material box, the liquid inlet pipe is connected to the inside of the material box, and a protective cover is provided at the other end of the liquid inlet pipe.

[0010] Preferably, a liquid pump is fixedly installed on the right side of the material box. The input end of the liquid pump is connected to the inside of the material box, and a conduit is fixedly installed on the output end of the liquid pump. The other end of the conduit is connected to a third bottle cap. The third bottle cap can be used interchangeably with the second bottle cap and can be threadedly connected to the feed pipe, so that the conduit is connected to the inside of the feed pipe.

[0011] Preferably, the first bottle cap, the second bottle cap, and the third bottle cap are all provided with sealing rubber strips inside.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] (1) The cell culture flask, through the combined use of the first cap, feed tube, second cap, third cap, sealing rubber strip and filter membrane, enables the device to filter the supplementary culture medium entering the culture flask through the filter membrane to prevent impurities that may exist inside from contaminating the cells. At the same time, the setting of the sealing rubber strip improves the sealing performance of the device, thereby further preventing the cells from being contaminated when the supplementary culture medium is added, and improving the practicality of the device.

[0014] (2) The cell culture flask and feeding mechanism are equipped with a material box and a stirring mechanism. The stirring mechanism is used in combination with a motor, mounting frame, stirring wheel, baffle, transmission box, first transmission gear and second transmission gear. This allows the device to start the stirring wheel by controlling the motor to rotate and revolve at the same time. Through the two rotations, the feeding culture medium located at the bottom of the material box can be quickly turned to the top, ensuring the uniformity of feeding and mixing. At the same time, the ultraviolet lamp can better disinfect the feeding culture medium, improving the practicality of the device. Attached Figure Description

[0015] The present invention will be further described below with reference to the accompanying drawings and embodiments:

[0016] Figure 1 This is a front view of the cell culture flask and feeding mechanism in this utility model;

[0017] Figure 2 This is a first cross-sectional view of the cell culture flask and feeding mechanism of this utility model;

[0018] Figure 3 This is a second sectional view of the cell culture flask and feeding mechanism in this utility model;

[0019] Figure 4 for Figure 2 Enlarged view of part A in the image;

[0020] Figure 5 for Figure 2 Enlarged view of part B in the image;

[0021] Figure 6 for Figure 3 Enlarged view of section C in the image;

[0022] Figure 7 This is a front view of the cell culture flask in this utility model.

[0023] Reference numerals: 1. Base plate; 2. Placement rack; 3. Culture flask; 4. Material bin; 5. Liquid inlet pipe; 6. Protective cap; 7. First cap; 8. Stirring mechanism; 801. Motor; 802. Mounting frame; 803. Stirring wheel; 804. Baffle; 805. Transmission box; 806. First transmission gear; 807. Second transmission gear; 9. Guide tube; 10. Feed pipe; 11. Second cap; 12. Sealing rubber strip; 13. Ultraviolet lamp strip; 14. Filter membrane; 15. Liquid pump; 16. Third cap. Detailed Implementation

[0024] This section will describe in detail the specific embodiments of the present utility model. The preferred embodiments of the present utility model are shown in the accompanying drawings. The purpose of the drawings is to supplement the textual description with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present utility model, but they should not be construed as limiting the scope of protection of the present utility model.

[0025] Please see Figure 1-7 This utility model provides a technical solution: a cell culture flask and a feeding mechanism. The culture flask 3 has a first cap 7 threaded onto its neck. Feed pipes 10 are provided on both the left and right sides of the culture flask 3. Second caps 11 are threaded onto the feed pipes 10. A filter membrane 14 is installed inside the feed pipes 10. When adding supplementary culture medium, the filter membrane 14 can filter the supplementary culture medium to prevent any impurities that may be present inside from contaminating the cells. The feeding mechanism includes a base plate 1, a material box 4 fixedly installed on the top of the base plate 1, a stirring mechanism 8 installed on the material box 4, and a conduit 9 that connects to the culture flask 3. A placement rack 2 is fixedly installed on the base plate 1 for placing... The frame 2 is used to fix the culture bottle 3. The stirring mechanism 8 includes a motor 801 fixedly installed on the front side of the material box 4. The stirring mechanism 8 also includes a mounting frame 802, a stirring wheel 803, a baffle 804, a transmission box 805, a first transmission gear 806 and a second transmission gear 807. This allows the device to drive the stirring wheel 803 to rotate and revolve simultaneously by controlling the motor 801 to start. Through these two rotations, the device can quickly turn the feed culture medium located at the bottom to the top. This not only makes the feed culture medium evenly mixed, but also allows the ultraviolet lamp strip 13 to better sterilize the feed culture medium, thus improving the practicality of the device.

[0026] like Figure 3 As shown, multiple sets of ultraviolet lamp strips 13 are fixedly installed on the inner top of the material box 4 and are distributed in a front-to-back manner. This allows the device to sterilize the supplementary culture medium by controlling the activation of the ultraviolet lamp strips 13, preventing it from contaminating the cells and improving the practicality of the device.

[0027] like Figure 4 and Figure 5 As shown, the first cap 7, the second cap 11, and the third cap 16 are all equipped with sealing rubber strips 12 inside. The sealing rubber strips 12 improve the sealing performance of the device, reduce the possibility of cell contamination, and improve the practicality of the device.

[0028] like Figure 2As shown, an inlet pipe 5 is fixedly installed on the left side of the material tank 4, communicating with the interior of the material tank 4. A protective cap 6 is provided at one end of the inlet pipe 5. A liquid pump 15 (with a switch button) is fixedly installed on the right side of the material tank 4. The input end of the liquid pump 15 communicates with the interior of the material tank 4, and a conduit 9 is fixedly installed at the output end of the liquid pump 15. The other end of the conduit 9 is connected to a third cap 16. The third cap 16 can be replaced with the second cap 11 and can be threadedly connected to the inlet pipe 10, allowing the conduit 9 to communicate with the interior of the inlet pipe 10. When the cell culture flask needs to be replenished with culture medium, the second cap 11 is replaced with the third cap 16, and the culture flask 3 is placed on the placement rack 2. The placement rack 2 supports and fixes the culture flask 3. Then, by activating the stirring mechanism and the liquid pump 15, the device can automatically draw the replenished culture medium into the culture flask 3 via the liquid pump 15, reducing cell contamination during operation and improving the practicality of the device.

[0029] like Figure 7 As shown, after the feeding culture medium is added, the third cap 16 is replaced with the second cap 11, and the culture bottle 3 is removed from the placement rack 2 of the feeding mechanism. Then, the culture bottle 3 is placed in the cell culture incubator to continue subsequent culture.

[0030] Working principle: In use, open the first cap 7 and place the cells into the culture flask 3. When it is necessary to add supplemental culture medium after a period of culture, place the culture flask 3 on the rack 2 for support and fixation. Then, open the protective cap 6 so that the operator can add supplemental culture medium into the material tank 4 through the inlet pipe 5. Start the ultraviolet lamp 13 to sterilize the supplemental culture medium. Start the motor 801 so that the motor 801 can drive the stirring wheel 803 to rotate through the mounting frame 802. Through the meshing of the first transmission gear 806 and the second transmission gear 807, the stirring wheel 803 rotates. 03. While revolving around the sun, the device also rotates on its own axis, thus reciprocating to agitate the culture medium. This agitates the culture medium at the bottom to the top, improving sterilization and mixing, thereby increasing the sterilization efficiency and uniformity of the culture medium. The second cap 11 of culture flask 3 is then replaced with the third cap 16, connecting culture flask 3 to the feed tank 4 of the feeding mechanism. The liquid pump 15 is then activated, drawing the sterilized culture medium through the conduit 9 into the feed pipe 10. The filter membrane 14 further filters the culture medium, preventing cell contamination from impurities. Finally, the culture medium is automatically added to culture flask 3. After adding the culture medium, the third cap 16 is replaced with the second cap 11, and culture flask 3 is removed from the feed mechanism's rack 2. Culture flask 3 is then placed in the cell culture incubator for further culture.

[0031] The embodiments of the present utility model have been described in detail above with reference to the accompanying drawings. However, the present utility model is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present utility model.

Claims

1. A cell culture flask and a feeding mechanism, characterized in that, The culture flask (3) has a first cap (7) threaded onto its mouth. The culture flask (3) has a feed pipe (10) on both the left and right sides. The feed pipe (10) has a second cap (11) threaded onto its surface. The feed pipe (10) has a filter membrane (14) inside its interior. The feeding mechanism includes a base plate (1) and a material box (4) fixedly mounted on the top of the base plate (1), as well as a stirring mechanism (8) and a conduit (9) that can connect to the culture flask (3) on the material box (4). A placement rack (2) is fixedly mounted on the base plate (1) and is used to fix the culture flask (3).

2. The cell culture flask and feeding mechanism according to claim 1, characterized in that: The agitation mechanism (8) includes a motor (801) fixedly installed on the front side of the material box (4), and also includes a mounting frame (802), an agitator (803), a baffle (804), a transmission box (805), a first transmission gear (806), and a second transmission gear (807). A set of mounting frames (802) is rotatably installed on the inner walls of both the front and rear sides of the material box (4). An agitator (803) is rotatably installed between the mounting frames (802). The output end of the motor (801) extends into the interior of the material box (4) and is fixedly connected to the mounting frame (802). A transmission box (805) is fixedly installed on the rear side of the material box (4). A through groove is provided on the rear side of (4), and the interior of the material box (4) is connected to the interior of the transmission box (805) through the through groove. A baffle (804) is rotatably installed on the front inner wall of the transmission box (805). One end of the agitator (803) extends through the through groove through the baffle (804) to the interior of the transmission box (805) and is fixedly installed with a second transmission gear (807). A first transmission gear (806) is fixedly installed on the rear inner wall of the transmission box (805). One end of the first transmission gear (806) is rotatably connected to the baffle (804), and the first transmission gear (806) meshes with the second transmission gear (807).

3. The cell culture flask and feeding mechanism according to claim 1, characterized in that: Multiple sets of ultraviolet lamp strips (13) are fixedly installed on the inner top of the material box (4) and are distributed in a front-to-back manner.

4. The cell culture flask and feeding mechanism according to claim 1, characterized in that: A liquid inlet pipe (5) is fixedly installed on the left side of the material box (4). The liquid inlet pipe (5) is connected to the inside of the material box (4). A protective cover (6) is provided at the other end of the liquid inlet pipe (5).

5. The cell culture flask and feeding mechanism according to claim 4, characterized in that: A liquid pump (15) is fixedly installed on the right side of the material box (4). The input end of the liquid pump (15) is connected to the inside of the material box (4). A conduit (9) is fixedly installed on the output end of the liquid pump (15). The other end of the conduit (9) is connected to a third bottle cap (16). The third bottle cap (16) can be used interchangeably with the second bottle cap (11) and can be threadedly connected to the feed pipe (10) so that the conduit (9) is connected to the inside of the feed pipe (10).

6. The cell culture flask and feeding mechanism according to claim 5, characterized in that: The first bottle cap (7), the second bottle cap (11), and the third bottle cap (16) are provided with sealing rubber strips (12).

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