Cell culture fluid infusion device

Abstract

The utility model discloses a fluid infusion device for cell culture, which particularly relates to the technical field of cell culture and comprises a main box body, an upper isolation plate, a middle isolation plate and a lower isolation plate are sequentially fixed in the main box body from top to bottom, and a stirring component is mounted at the top end of the middle isolation plate. An intermittent liquid supplementing assembly is arranged in the main box body and located below the lower isolation plate. The auxiliary box body is fixed to the bottom of the lower isolation plate, a limiting block is arranged in the auxiliary box body, when liquid needs to be added, an auxiliary shaft motor is started, the auxiliary shaft motor drives an auxiliary rotating shaft to enable a driving wheel to rotate, the driving wheel drives a synchronous wheel to enable a rotating block to rotate, and the rotating block enables a supporting plate to move up and down; the supporting plate drives the supporting rod to enable the limiting block to move up and down, the limiting block moves up and down with frequency to enable mixed liquid to be supplemented through the liquid discharging pipe, liquid supplementing is automatically conducted, the liquid supplementing efficiency is improved, and manpower waste is reduced.

Description

Technical Field

[0001] This utility model relates to the field of cell culture, specifically to a cell culture fluid replenishment device. Background Technology

[0002] Cell culture refers to a method of enabling organisms to survive, grow, reproduce, and maintain their main structures and functions by simulating the in vivo environment (sterile, suitable temperature, pH, and certain nutritional conditions, etc.) outside the body. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technique. Whether for bioengineering as a whole or for biological cloning technology, cell culture is an indispensable process; cell culture itself is the large-scale cloning of cells.

[0003] Therefore, in order to maintain the normal functioning of cells, it is necessary to replenish the cells with fluid regularly. However, in actual use, the main technical problem is that traditional fluid replenishment devices often require manual replenishment at regular intervals. This often cannot effectively control the amount and time of replenishment, and also wastes a lot of manpower and reduces the efficiency of replenishment. Utility Model Content

[0004] The purpose of this invention is to provide a cell culture fluid replenishment device to address the aforementioned shortcomings in the technology.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a cell culture fluid replenishment device, comprising a main body, wherein an upper isolation plate, a middle isolation plate and a lower isolation plate are fixed inside the main body from top to bottom, a stirring assembly is installed at the top of the middle isolation plate, and an intermittent fluid replenishment assembly is provided inside the main body and below the lower isolation plate;

[0006] The secondary housing is fixed to the bottom of the lower partition plate, and a limit block is provided inside the secondary housing.

[0007] Preferably, the intermittent fluid replenishment assembly includes a secondary shaft motor, which is fixed inside the main housing and located below the lower isolation plate. A secondary rotating shaft is located inside the main housing and below the lower isolation plate. The output shaft of the secondary shaft motor is connected to the secondary rotating shaft. A drive wheel is fixed to the other end of the secondary rotating shaft. A rotating rod is mounted inside the main housing and below the lower isolation plate via a bearing. A driven wheel is fixedly connected to the surface of the rotating rod at its middle section. A synchronous belt is mounted on the surfaces of the drive wheel and the driven wheel. A rotating block is fixed to the surface of the rotating rod in front of the driven wheel. A support rod is fixedly connected to the bottom of the limiting block. The bottom end of the support rod extends to the lower part of the secondary housing and is fixed with a support plate. The bottom of the support plate contacts the rotating block. A spring is installed outside the support rod and below the secondary housing.

[0008] Preferably, the stirring assembly includes a main shaft motor, which is fixed to the top of the middle partition plate. A main rotating shaft is provided inside the main housing. The output shaft of the main shaft motor is connected to the main rotating shaft. The other end of the main rotating shaft extends to the top of the upper partition plate and is fixed with stirring blades.

[0009] Preferably, a funnel is fixed to the top of the main housing, an inlet valve is installed on the outside of the funnel, and the bottom end of the funnel extends into the interior of the main housing.

[0010] Preferably, a connecting pipe is fixed to the top of the middle isolation plate, a solenoid valve is installed on the outside of the connecting pipe, and both ends of the connecting pipe extend to the top of the upper isolation plate and the bottom of the middle isolation plate. A drain pipe is fixed to the outside of the main housing, and one end of the drain pipe extends into the interior of the main housing.

[0011] The technical effects and advantages provided by this utility model in the above technical solution are as follows:

[0012] 1. When liquid needs to be added, the speed of the secondary shaft motor is adjusted according to the required amount. The secondary shaft motor is started, and the secondary shaft motor drives the secondary rotating shaft, which in turn drives the driving wheel to rotate. The driving wheel drives the synchronous belt, which in turn drives the driven wheel to rotate. The driven wheel drives the rotating rod, which in turn drives the rotating block to rotate. The rotating block causes the support plate to move up and down. The support plate drives the support rod, which in turn causes the limit block to move up and down. The limit block moves up and down at a frequency, allowing the mixed liquid to be replenished through the drain pipe. This automated replenishment improves replenishment efficiency and reduces manpower waste.

[0013] 2. By pouring the prepared replenishing material into the funnel and starting the main shaft motor, the main shaft motor drives the stirring blades to rotate, thus mixing the material. The ratio of the replenishing material can be controlled automatically, making it convenient to adjust the material ratio according to different situations. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the front cross-sectional structure of this utility model;

[0017] Figure 3 This is a partial front view cross-sectional structural schematic diagram of the present invention;

[0018] Figure 4This is a schematic diagram of the drive wheel structure of this utility model;

[0019] Figure 5 This is a schematic diagram of the liquid inlet valve structure of this utility model.

[0020] Explanation of reference numerals in the attached figures:

[0021] 1. Main housing; 2. Upper partition plate; 3. Middle partition plate; 4. Lower partition plate; 5. Funnel; 6. Inlet valve; 7. Main shaft motor; 8. Main rotating shaft; 9. Stirring blade; 10. Connecting pipe; 11. Solenoid valve; 12. Secondary shaft motor; 13. Secondary rotating shaft; 14. Driving wheel; 15. Driven wheel; 16. Synchronous belt; 17. Rotating rod; 18. Drain pipe; 19. Secondary housing; 20. Limit block; 21. Rotating block; 22. Support plate; 23. Support rod; 24. Spring. Detailed Implementation

[0022] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings.

[0023] This utility model provides, for example Figures 1-5 The cell culture replenishment device shown includes a main chamber 1, inside which an upper isolation plate 2, a middle isolation plate 3 and a lower isolation plate 4 are fixed from top to bottom. A stirring assembly is installed at the top of the middle isolation plate 3. An intermittent replenishment assembly is installed inside the main chamber 1 and below the lower isolation plate 4. A secondary chamber 19 is fixed to the bottom of the lower isolation plate 4. A limit block 20 is installed inside the secondary chamber 19.

[0024] Furthermore, the intermittent fluid replenishment assembly includes a secondary shaft motor 12, which is fixed inside the main housing 1 and located below the lower isolation plate 4. A secondary rotating shaft 13 is provided inside the main housing 1 and below the lower isolation plate 4. The output shaft of the secondary shaft motor 12 is connected to the secondary rotating shaft 13. A drive wheel 14 is fixed to the other end of the secondary rotating shaft 13. A rotating rod 17 is installed inside the main housing 1 and below the lower isolation plate 4 via a bearing. A driven wheel 15 is fixedly connected to the surface of the rotating rod 17 at its middle position. A synchronous belt 16 is installed on the surfaces of the drive wheel 14 and the driven wheel 15. A rotating block 21 is fixed to the surface of the rotating rod 17 and in front of the driven wheel 15. A support rod 23 is fixedly connected to the bottom of the limiting block 20. The bottom end of the support rod 23 extends to the lower part of the secondary housing 19 and is fixed with a support plate 22. The bottom of the support plate 22 contacts the rotating block 21. A spring 24 is installed on the outside of the support rod 23 and below the secondary housing 19.

[0025] Furthermore, the stirring assembly includes a main shaft motor 7, which is fixed at the top of the middle partition plate 3. A main rotating shaft 8 is provided inside the main housing 1. The output shaft of the main shaft motor 7 is connected to the main rotating shaft 8. The other end of the main rotating shaft 8 extends to the top of the upper partition plate 2 and is fixed with stirring blades 9.

[0026] Furthermore, a funnel 5 is fixed to the top of the main housing 1, and an inlet valve 6 is installed on the outside of the funnel 5. The bottom end of the funnel 5 extends into the interior of the main housing 1.

[0027] Furthermore, a connecting pipe 10 is fixed to the top of the middle isolation plate 3, and a solenoid valve 11 is installed on the outside of the connecting pipe 10. Both ends of the connecting pipe 10 extend to the top of the upper isolation plate 2 and the bottom of the middle isolation plate 3. A drain pipe 18 is fixed to the outside of the main housing 1, and one end of the drain pipe 18 extends into the interior of the main housing 1.

[0028] Through the above technical solution:

[0029] In use, the prepared raw materials for replenishment are poured into the funnel 5, and the main shaft motor 7 is started. The main shaft motor 7 drives the main rotating shaft 8 to rotate the stirring blade 9, which stirs and mixes the raw materials. The solenoid valve 11 is opened to allow the mixture to enter between the middle isolation plate 3 and the lower isolation plate 4. When liquid needs to be added, the speed of the auxiliary shaft motor 12 is adjusted according to the required amount. The auxiliary shaft motor 12 is started, and the auxiliary shaft motor 12 drives the auxiliary rotating shaft 13 to rotate the drive wheel 14. The drive wheel 14 drives the synchronous belt 16 to rotate the driven wheel 15. The driven wheel 15 drives the rotating rod 17 to rotate the rotating block 21. The rotating block 21 causes the support plate 22 to move up and down. The support plate 21 drives the support rod 23 to move the limit block 20 up and down. The limit block 20 moves up and down at a frequency, allowing the mixture to be replenished through the drain pipe 18.

[0030] The foregoing description only illustrates certain exemplary embodiments of the present invention. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A cell culture fluid replenishment device, characterized in that, include: The main body (1) has an upper isolation plate (2), a middle isolation plate (3) and a lower isolation plate (4) fixed inside from top to bottom. A stirring assembly is installed at the top of the middle isolation plate (3). An intermittent liquid replenishment assembly is provided inside the main body (1) and below the lower isolation plate (4). Sub-box (19) is fixed to the bottom of the lower partition plate (4), and a limit block (20) is provided inside the sub-box (19).

2. The cell culture fluid replenishment device according to claim 1, characterized in that: The intermittent fluid replenishment assembly includes a secondary shaft motor (12), which is fixed inside the main housing (1) and located below the lower partition plate (4). A secondary rotating shaft (13) is provided inside the main housing (1) and below the lower partition plate (4). The output shaft of the secondary shaft motor (12) is connected to the secondary rotating shaft (13). A drive wheel (14) is fixed at the other end of the secondary rotating shaft (13). A rotating rod (17) is installed inside the main housing (1) and below the lower partition plate (4) via a bearing. The rotating rod (17) is located on the surface of the surface of the rotating rod (17) at the middle position. A driven wheel (15) is fixedly connected. A synchronous belt (16) is installed on the surface of the driving wheel (14) and the driven wheel (15). A rotating block (21) is fixed on the surface of the rotating rod (17) and in front of the driven wheel (15). A support rod (23) is fixedly connected to the bottom of the limiting block (20). The bottom end of the support rod (23) extends to the lower part of the auxiliary housing (19) and is fixed with a support plate (22). The bottom of the support plate (22) is in contact with the rotating block (21). A spring (24) is installed on the outside of the support rod (23) and below the auxiliary housing (19).

3. The cell culture fluid replenishment device according to claim 1, characterized in that: The stirring assembly includes a main shaft motor (7), which is fixed at the top of the middle partition plate (3). The main housing (1) is provided with a main rotating shaft (8). The output shaft of the main shaft motor (7) is connected to the main rotating shaft (8). The other end of the main rotating shaft (8) extends to the top of the upper partition plate (2) and is fixed with stirring blades (9).

4. The cell culture fluid replenishment device according to claim 1, characterized in that: The top of the main body (1) is fixed with a funnel (5), and an inlet valve (6) is installed on the outside of the funnel (5). The bottom end of the funnel (5) extends into the interior of the main body (1).

5. The cell culture fluid replenishment device according to claim 1, characterized in that: A connecting pipe (10) is fixed to the top of the middle isolation plate (3). A solenoid valve (11) is installed on the outside of the connecting pipe (10). Both ends of the connecting pipe (10) extend to the top of the upper isolation plate (2) and the bottom of the middle isolation plate (3). A drain pipe (18) is fixed to the outside of the main box (1). One end of the drain pipe (18) extends into the inside of the main box (1).

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