Extraction module

Abstract

The utility model discloses an extraction module, which relates to the technical field of medical instruments and information intelligent equipment, and is characterized in that a first lifting substrate and a second lifting substrate are respectively connected with a magnetic bar unit and a magnetic bar sleeve loading unit, and the two lifting substrates are connected to the same vertical slide rail; the first substrate driving motor and the second substrate driving motor respectively drive the two lifting substrates to move, so that the magnetic bar unit can penetrate through the hollow part of the magnetic bar sleeve loading unit to be inserted into different positions in the connected magnetic bar sleeve. Therefore, the magnetic field around the magnetic bar sleeve is changed to meet different requirements of mixing and magnetic bead adsorption transfer in nucleic acid extraction operation. Besides, the vertical base plate can be driven by the transverse driving mechanism, and then the two connected lifting base plates are driven to synchronously and transversely move, so that transverse movement control of the two different functional units is simpler and more reliable.

Description

Technical Field

[0001] This utility model relates to the field of medical devices and intelligent information equipment, specifically to an extraction module. Background Technology

[0002] Diagnostic medical technology is the prerequisite for the implementation of precision medicine projects that benefit people's livelihoods. Precision medicine can largely avoid the serious problems of various microbial resistance caused by overuse of drugs. Molecular diagnostics, an important branch of diagnostic technology, has completely changed the detection of pathogenic or non-pathogenic microorganisms in industrial sectors such as food, clinical, water treatment, and agriculture through the advancement of PCR and its different variants for nucleic acid detection. Especially in clinical and disease control scenarios related to human life and health, molecular diagnostics can accurately identify earlier stages of infection by detecting nucleic acids. However, in current diagnostic technologies, nucleic acid extraction and amplification equipment are usually separate instruments, and the operation process requires a large amount of manual intervention. On the one hand, some target detection poses a certain risk of contamination to the operator; on the other hand, it also requires higher skill levels from the operator. Currently, extracting nucleic acids from samples containing nucleic acids and ensuring that the extracted base sequence length is qualified is the ultimate goal pursued by various enterprises, which is also conducive to obtaining high-accuracy amplification detection results.

[0003] In the design of various automated equipment, the extraction quality of the automated extraction module directly affects the quality of amplification results. To support high-throughput automated biosample processing systems for fully automated molecular diagnostics, domestic and international manufacturers have designed different types of extraction procedures. US Patent 20240279639A1 discloses a fully automated sample-to-result processing device with integrated consumables. This device integrates pipetting consumables, extraction kits, and the final amplification detection kit. The extraction technology uses magnetic beads, but the magnetic force is applied to the wall of the extraction well. To improve extraction efficiency, this technology requires an additional mixing and stirring unit, but the actual extraction efficiency is difficult to improve. US Patent 20240255536A1 discloses a special type of adsorption scheme for extracting nucleic acid sequences from samples. This device requires a metal, non-disposable sample transfer needle. While the extraction method is novel and simple to operate, it is not suitable for samples with a high risk of cross-contamination. Chinese invention patents... The scheme disclosed in CN108588066B involves loading magnetic units onto the wall of a deep-well plate consumable, configured as an array of multiple magnetic pillar structures that can be inserted into the wall of each well of the deep-well plate consumable. To ensure thorough mixing of the magnetic beads with reagents or samples during the extraction process, a vibration device is also included to vibrate for a preset time when mixing is required. The scheme disclosed in US Patent US9650626B2 designs a magnetic bead extraction scheme that uses a magnetic rod and a magnetic rod sleeve. To reduce contamination during the mixing process, the edge area of ​​the magnetic rod sleeve is enlarged and designed as a cover structure that can be used with the extraction reaction wells to perform a low-contamination extraction operation with the inserted magnetic rod. However, in actual use, this magnetic rod sleeve occupies too much storage space, and to improve extraction efficiency, the magnetic rod sleeve generally needs to perform complex trajectory movements, which causes motion interference from the cover, and its contamination protection effect is not good. These domestic and foreign manufacturers have explored different types of extraction schemes, hoping to obtain a low-contamination, high-efficiency, and automated extraction scheme; however, the results are still far from the expected values.

[0004] Therefore, it is necessary to optimize the design of the nucleic acid extraction module, focusing on aspects such as control simplicity and efficiency, final product compliance and higher yield. Designing an extraction module that can automatically perform all extraction operations and further adapting it to perform high-throughput detection within an automated biological sample processing system is an urgent technical problem to be solved. Utility Model Content

[0005] The purpose of this utility model is to address the aforementioned problems by providing an extraction module that employs two movable lifting base plates, each equipped with a magnetic rod loading unit and a magnetic rod unit, and allows both to slide on the same common vertical slide rail. This naturally makes the motion reference of the different lifting base plates the same, which is more conducive to standardized control to meet the high-precision coordination requirements of the magnetic rod unit and the magnetic rod loading unit during the extraction process.

[0006] The technical solution adopted in this utility model is as follows:

[0007] An extraction module includes an extraction module body. The extraction module body contains a first lifting base plate, a first base plate drive motor, a first base plate transmission screw, a second lifting base plate, a second base plate drive motor, a second base plate transmission screw, a vertical slide rail, and a vertical base plate. A magnetic rod unit is connected to the first lifting base plate, and a magnetic rod sleeve loading unit is connected to the second lifting base plate. The magnetic rod sleeve loading unit is hollow, and one end can be connected to a magnetic rod sleeve. The magnetic rod unit can pass through the magnetic rod sleeve loading unit and extend into the magnetic rod sleeve. The vertical slide rail is connected to the vertical base plate. The first and second lifting base plates are movably connected to the vertical slide rail via sliders, and the first lifting base plate is always positioned at a higher position relative to the second lifting base plate. The first base plate drive motor is mounted on the second lifting base plate and connected to the first lifting base plate via the first base plate transmission screw. The first base plate drive motor can drive the first base plate to move relative to the second base plate along the vertical slide rail, changing the relative distance between the first and second lifting base plates.

[0008] It also includes a second substrate drive motor and a second substrate transmission screw. The second substrate drive motor is mounted on the vertical substrate and connected to the second lifting substrate through the second substrate transmission screw. The second substrate drive motor can drive the first substrate and the second substrate to move synchronously along the vertical slide rail. The first substrate transmission screw and the second substrate transmission screw are arranged in opposite directions in an inverted manner.

[0009] Furthermore, the extraction module body also includes a horizontal driving mechanism, which is connected to the vertical substrate and can drive the vertical substrate to move horizontally. The horizontal driving mechanism includes a horizontal driving motor, a horizontal lead screw, a horizontal slide rail, a horizontal connecting block, and a horizontal slider. The horizontal driving motor is connected to the horizontal lead screw, and the horizontal lead screw is threadedly connected to the horizontal connecting block. The bottom end of the horizontal connecting block is connected to the horizontal slider, and the top end is connected to the vertical substrate. The horizontal slider is connected to the horizontal slide rail arranged parallel to the horizontal lead screw, and the horizontal slider can move along the extension direction of the horizontal slide rail under the drive of the horizontal driving motor.

[0010] Furthermore, the extraction module body also includes a box loading position, which includes a loading platform that can move horizontally and enter and exit the extraction module body, and a loading drive mechanism that can drive the loading platform to move. The top of the loading platform is equipped with a box limiting member, which can limit the extraction reagent kit inside.

[0011] Furthermore, the loading drive mechanism includes a loading drive motor, a loading transmission gear, and a loading transmission rack. The loading drive motor is connected to the loading transmission gear, and the loading transmission rack is disposed on the side wall of the loading platform. The loading transmission gear meshes with the loading transmission rack.

[0012] Furthermore, the extraction module body is equipped with a shell composed of thin-shell components, and the shell has a storage opening, on which a module compartment door is elastically fastened; the loading platform has rollers at the bottom; when the loading platform moves outward from the shell, the module compartment door can be pushed open by the loading platform, and the rollers at the bottom of the loading platform can slide on the module compartment door with low resistance; when the loading platform retracts into the shell, the module compartment door can be fastened to the storage opening on the shell under the action of elastic force.

[0013] Furthermore, a fan assembly is disposed on the top of the housing, and a filter element is provided at the air outlet of the fan assembly.

[0014] Furthermore, the box-limiting component is also equipped with a pyrolysis heating element and an elution heating element.

[0015] Furthermore, the transverse lead screw is arranged along the Y-axis, the loading transmission rack is arranged along the X-axis, and the drive shaft of the loading drive motor is inverted downwards and connected to the loading transmission gear.

[0016] Furthermore, the second lifting base plate is also equipped with a rotary drive mechanism, which includes a rotary drive motor, a rotary drive gear, an intermediate gear and a rotary transmission gear. The rotary transmission gear is located at the end of the magnetic rod sleeve loading unit. Several magnetic rod sleeve loading units are arranged side by side, and the rotary transmission gears corresponding to adjacent magnetic rod sleeve loading units mesh with each other. The rotary drive gear is meshed with the intermediate gear, and the intermediate gear is meshed with any rotary transmission gear.

[0017] Furthermore, a detection assembly plate is connected to the second lifting base plate. The detection assembly plate has a sensing sheet on the side close to the second lifting base plate along its length and a detector on the side away from the second lifting base plate. A sensing element is arranged on the second base plate drive motor. The sensing sheet and the sensing element cooperate with each other to limit the highest position of the second lifting base plate. The detector is spaced at a preset distance from the second lifting base plate. The detector can be matched with the first lifting base plate to limit the maximum distance between the first lifting base plate and the second lifting base plate.

[0018] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0019] 1. This utility model connects a magnetic rod unit and a magnetic rod sleeve loading unit via a first lifting base plate and a second lifting base plate, respectively. The two lifting base plates are connected to the same vertical slide rail, ensuring consistent motion reference and precision. The first and second base plate drive motors drive the two lifting base plates, allowing the magnetic rod unit to pass through the hollow portion of the magnetic rod sleeve loading unit and insert into different positions within the connected magnetic rod sleeve. This alters the magnetic field around the magnetic rod sleeve to accommodate different requirements in nucleic acid extraction operations, such as mixing and magnetic bead adsorption and transfer. Furthermore, the vertical base plate can be driven by a lateral drive mechanism, causing the two connected lifting base plates to move laterally synchronously. This simplifies and simplifies the lateral movement control of the two different functional units.

[0020] 2. The first and second lifting base plates are driven by a first base plate drive motor and a second base plate drive motor, respectively. The second lifting base plate is also connected to the first base plate drive motor, resulting in higher control precision for the lifting motion and simplifying the implementation of synchronous actions in nucleic acid extraction scenarios (synchronous lifting and lowering of the magnetic rod sleeve and the magnetic rod can be achieved with a single motor drive). A vertical slide rail is also arranged between the two lifting base plates, enabling them to be driven precisely without deflection during lifting and lowering.

[0021] 3. The rotary drive mechanism arranged on the second lifting base plate can convert the motion output by the rotary drive motor into the rotational motion of the magnetic rod sleeve loading unit, which in turn drives the connected magnetic rod sleeve to rotate. This introduces a specific rotational motion into the extraction operation, allowing the magnetic beads and liquid to be more thoroughly mixed. The high-precision gear transmission formed by the rotary drive motor-driven rotary drive gear, intermediate gear, and rotary transmission gear can precisely control the rotational speed, achieving sufficient vortexing without generating excessive turbulence that could cause splashing. The number of magnetic rod sleeve loading units and magnetic rod units is equal and multiple, enabling the simultaneous extraction of more objects to achieve high-throughput extraction processing.

[0022] 4. The detectors connected at fixed intervals on the second lifting plate can precisely limit the maximum distance between the first and second lifting plates, thus accurately and consistently limiting the distance the magnetic rod is pulled away from the bottom of the magnetic rod sleeve. This makes the demagnetization state of the magnetic rod sleeve more reliable and prevents interference with the magnetic beads in the holes during the mixing process. The cassette loading position can be automatically driven by the loading drive mechanism, cooperating with the loading of the extraction kit, enabling the extraction module to operate autonomously in conjunction with the automated system. Attached Figure Description

[0023] Figure 1 This is a diagram showing the overall structure of the extraction module;

[0024] Figure 2 This is a schematic diagram of the rotary drive mechanism;

[0025] Figure 3 This is a schematic diagram of the interlocking structure of rotary transmission gears;

[0026] Figure 4 This is a state diagram of the magnetic rod unit passing through the hollow magnetic rod sleeve loading unit;

[0027] Figure 5 This is a schematic diagram of the lateral movement drive structure;

[0028] Figure 6 This is a structural diagram of the box loading bit;

[0029] Figure 7 This is a diagram illustrating the process by which the box loading position is driven to extend beyond the main body of the extraction module block;

[0030] Figure 8 This is a diagram illustrating the process of receiving and extracting the kit at the loading position.

[0031] Figure 9 This is a diagram illustrating the process of the box loading position being driven back to the main body of the extraction module;

[0032] Figure 10 This is a diagram illustrating the process by which the magnetic rod sleeve loading unit is driven to take up the loaded magnetic rod sleeve within the extraction kit 30.

[0033] Figure 11 This is a diagram illustrating the process of the magnetic rod sleeve loading unit completing the loading of the magnetic rod sleeve.

[0034] Figure 12 This is a diagram showing the lysis process performed when a magnetic rod is placed inside the lysis well of the extraction kit.

[0035] Figure 13 This is a diagram showing the elution process as the loaded magnetic rod is placed inside the elution well of the extraction kit.

[0036] Figure 14 This is a complete picture of the extraction module configured inside the casing.

[0037] In the diagram, the markings are as follows: 10-Magnetic rod unit, 11-First substrate drive motor, 12-First substrate transmission screw, 13-First lifting substrate, 14-Sensing sheet, 15-Detector, 20-Magnetic rod sleeve loading unit, 21-Second substrate drive motor, 22-Second substrate transmission screw, 23-Second lifting substrate, 24-Sensing sheet, 25-Sensing element, 30-Extraction kit, 31-Rotary drive motor, 32-Rotary drive gear, 34-Intermediate gear, 331, 332, 333, 334-Rotary transmission gears, 41-Loading drive unit. 42-Loading transmission gear, 43-Loading transmission rack, 50-Box loading position, 51-Horizontal drive motor, 52-Horizontal lead screw, 53-Horizontal connecting block, 100-Outer shell, 151-Detection assembly plate, 201-Hollow section, 202-Bearing, 501-Roller, 502-Module compartment door, 503-Pyrolysis heating element, 504-Eluting heating element, 505-Box limiting element, 60-Vertical base plate, 601-Vertical slide rail, 602-Horizontal slider, 603-Horizontal slide rail, 70-Fan assembly, 701-Filter element. Detailed Implementation

[0038] The present invention will now be described in detail with reference to the accompanying drawings.

[0039] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0040] Example 1

[0041] An extraction module, such as Figure 1-5 As shown, Figure 1This is an overall structural diagram of the extraction module. The extraction module includes an upper half capable of lifting and lowering, and a lower half capable of telescopic movement. The upper half includes a vertical base plate 60, on which a vertical slide rail 601 is fixedly connected. The preset extension height of the vertical base plate 60 can be set to 1.5 to 3 times the length of the magnetic rod unit 10 connected to it. This ensures that the magnetic rod unit 10 has sufficient lifting stroke while preventing the lifting time from being too long and affecting extraction efficiency or control accuracy. At different positions on the vertical slide rail 601, a first lifting base plate 13 and a second lifting base plate 23 are respectively connected by two sliding blocks. To ensure that the first lifting base plate 13 and the second lifting base plate 23 operate accurately and without interference on the same vertical slide rail 601, the first lifting base plate 13 and the second lifting base plate 23 have a relative height configuration. The first lifting base plate 13 is always positioned at a higher position on the vertical slide rail 601 than the second lifting base plate 23. A magnetic rod unit 10 is fixedly connected to the first lifting base plate 13, and the second lifting base plate 23... A magnetic rod sleeve loading unit 20 is fixedly connected to the substrate 23. This allows the first lifting substrate 13 at a higher position to rise independently while keeping the second lifting substrate 23 at a lower position stationary. Under basically static conditions, the magnetic field around the magnetic rod sleeve connected to the magnetic rod sleeve loading unit 20 can be weakened or even completely demagnetized in the bottom area, making it more suitable for efficient extraction operations. The first lifting substrate 13 is driven by the first substrate drive motor 11. Here, the output of the first substrate drive motor 11 is connected to the first substrate drive screw 12. The first substrate drive screw 12 is also directly or indirectly threadedly connected to the first lifting substrate 13. For example, the first lifting substrate 13 can be locally configured with threads to directly connect to the first substrate drive screw 12, or a threaded connecting block can be connected to the first lifting substrate 13 to indirectly connect to the first substrate drive screw 12. In this way, the rotational motion of the first substrate drive motor 11 can be converted into the motion of the first lifting substrate 13 sliding up and down along the vertical slide rail 601.The second lifting base plate 23 employs a similar lifting motion drive. Here, the output of the second base plate drive motor 21 is connected to the second base plate transmission screw 22. The second base plate transmission screw 22 is also directly or indirectly threadedly connected to the second lifting base plate 23, which can convert the rotational motion of the second base plate drive motor 21 into the up-and-down sliding motion of the second lifting base plate 23 along the vertical slide rail 601. The second lifting base plate 23 is also fixedly connected to the first base plate drive motor 11. Thus, the second lifting base plate 23 can also drive the first lifting base plate 13 to move synchronously at a fixed interval. This enables the magnetic rod unit 10 and the magnetic rod sleeve to move synchronously during extraction operations, such as magnetic bead transfer and mixing. The magnetic rod unit 10 is connected to the magnetic rod sleeve loading unit 20. The magnetic field generated around the connected magnetic rod sleeve will not change. Using a single motor to achieve synchronous lifting of the first lifting base plate 13 and the second lifting base plate 23 is low-difficulty and highly reliable. The first base plate drive motor 11 and the second base plate drive motor 21 are arranged in an opposite orientation so that the connected first base plate transmission screw 12 and the second base plate transmission screw 22 face opposite directions, improving the space utilization of the limited space. The second base plate drive motor 21 is fixedly connected to the vertical base plate 60 of the fixed vertical slide rail 601. A sensor 25 is also arranged on the second base plate drive motor 21. The sensor 25 can acquire distance data from the second lifting base plate 23 to the second base plate drive motor 21, and limit the second lifting base plate 23 based on the acquired distance data. The highest position of the lifting base plate 23 can also be used to calibrate the movement position of the second lifting base plate 23. The second lifting base plate 23 is also fixedly connected to the detection assembly plate 151. The detection assembly plate 151 and the reference surface of the second lifting base plate 23 are fixedly connected to the detector 151 at a preset distance. When the first lifting base plate 13 moves to the same height as the detector 15, the detector 15 is triggered by the sensing plate 14 connected to the first lifting base plate 13, thereby limiting the maximum distance between the first lifting base plate 13 and the second lifting base plate 23. The maximum distance here is set to the maximum distance of the magnetic rod unit 10 pulling away from the magnetic rod sleeve, which can be 10-20 times the average hydraulic diameter of the magnetic rod sleeve, so that the magnetic rod sleeve is demagnetized more thoroughly, and can also serve as the first lifting base plate 13. 3. The reference calibrator position for the lifting motion: To simplify control, the sensing plate 24 that cooperates with the sensing element 25 is fixedly connected to the detection assembly plate 151. Of course, a part of the detection assembly plate 151 can also be directly processed into the sensing plate 24. The second lifting base plate 23 is also equipped with a rotary drive mechanism. The rotary drive mechanism includes a rotary drive motor 31, which can drive the magnetic rod sleeve loading unit 20 to rotate through a rotary transmission mechanism, thereby driving the connected magnetic rod sleeve to rotate. By changing the speed of the drive motor 31, different speeds can be output to adapt to the magnetic bead release sub-step and the magnetic bead mixing sub-step. The magnetic bead release sub-step can be configured with a higher first speed, and the mixing sub-step with a lower second speed.The lower half of the extraction module also includes a cassette loading position 50, which can be driven by the loading drive motor 41 to extend beyond the main body of the extraction module to receive the extraction reagent kit 30. To reduce the movement resistance of the cassette loading position 50, at least one roller 501 is also provided at its bottom. The vertical base plate 60 of the extraction module can be threadedly connected to the horizontal lead screw 52 of the horizontal drive mechanism via a horizontal connecting block 53, allowing the vertical base plate 60 to slide along the extension direction of the horizontal slide rail 603. Here, the vertical base plate 60 can also engage with the horizontal slide rail 603 via a horizontal slider 602.

[0042] Figure 2 This is a schematic diagram of the rotary drive mechanism. Figure 3 This is a schematic diagram of the interlocking structure of the rotary transmission gears. The second lifting base plate 23 is also fixedly connected to a rotary drive motor 31. For ease of installation and reliable transmission, the rotary drive motor 31 is inverted and mounted on the second lifting base plate 23. The distance between the rotary drive motor 31 and the plane of the second lifting base plate 23 is preset. The output shaft of the rotary drive motor 31 is connected to a rotary drive gear 32. To obtain a suitable transmission ratio and more reliable and efficient transmission, the rotary drive gear 32 is also meshed with an intermediate gear 34. The intermediate gear 34 can directly mesh with the rotary transmission gears 331, 332, 333, or 334 mounted on one end region of any magnetic rod sleeve loading unit 20. There are four magnetic rod sleeve loading units 20, each with a rotary transmission gear 331, 332, 333, and 334 connected to one end region. The four rotary transmission gears 331, 332, 333, and 334 are connected in pairs. In order to reduce the resistance in the rotational motion of the magnetic rod sleeve loading unit 20, a bearing 202 is also arranged in the end area of ​​each magnetic rod sleeve loading unit 20 connected to the rotary transmission gears 331, 332, 333 and 334. In order to cooperate with the magnetic rod unit 10 to perform nucleic acid extraction operation, each magnetic rod sleeve loading unit 20 has a hollow part 201 inside, so that the magnetic rod unit 10 can pass through the magnetic rod sleeve loading unit 20. The number of magnetic rod units 10 and magnetic rod sleeve loading units 20 is the same, which can be 3, 4, 5, 6, 7, 8, etc. Here, the rotary drive gear 32 is indirectly meshed with each intermediate gear 34 and connected to any one of the four rotary transmission gears 331, 332, 333 or 334. In this way, the rotational motion output by the rotary drive motor 31 can be converted into the rotational motion of the multiple magnetic rod sleeve loading units 20 connected by the rotary transmission gears 331, 332, 333 and 334.

[0043] Figure 4This is a diagram showing the state of the magnetic rod unit 10 passing through the hollow magnetic rod sleeve loading unit 20. To clearly illustrate the state of the magnetic rod unit 10 passing through the magnetic rod sleeve loading unit 20, the lower part of the magnetic rod sleeve loading unit 20 is not connected to the magnetic rod sleeve. During this process, the second substrate drive motor 21 rotates to raise the second lifting substrate 23 along the vertical slide rail 601 by a predetermined distance. Since it is also fixedly connected to the first drive motor 11, it can indirectly drive the first lifting substrate 13 to rise synchronously by a predetermined distance. Before this, the first substrate drive motor 11 can rotate to change the distance between the first lifting substrate 13 and the second lifting substrate 23. Here, the first substrate drive motor 11 can drive the first lifting substrate 13 to descend, thus... The magnetic rod unit 10 penetrates more of the hollow part of the magnetic rod sleeve loading unit 20, and more magnetic rod units 10 protrude from the lower end of the magnetic rod sleeve loading unit 20. This state can be used in the scenario of magnetic bead transfer. After the mixing is completed, the first substrate drive motor 11 can drive the first lifting substrate 13 to descend, so that the distance between the first lifting substrate 13 and the second lifting substrate 23 becomes smaller. The magnetic field of the magnetic rod sleeve connected to the lower part of the magnetic rod sleeve, especially the bottom area of ​​the magnetic rod sleeve, is enhanced, which can attract magnetic beads. After the attraction is completed, the second lifting substrate 23 can be driven to rise, thereby driving the first lifting substrate 13 to rise synchronously. Under the premise that the magnetic field around the magnetic rod sleeve remains unchanged, the magnetic beads are kept in the state of being attracted and rise.

[0044] Figure 5 This is a schematic diagram of the lateral movement drive structure; the upper part of the structure is omitted here, only the lower part of the lateral movement drive and box loading position 50 drive structure is shown. The lateral drive motor 51 is arranged horizontally and can be connected to the base plate of the extraction module via a fixed base. The output shaft of the lateral drive motor 51 is connected to the lateral lead screw 52. A lateral connecting block 53 is threaded onto the lateral lead screw 52. The bottom end of the lateral connecting block is fixedly connected to the lateral slider 602, and the top end is connected to the vertical base plate 60. The lateral slider 602 is also fitted with a lateral slide rail 603 arranged parallel to the lateral lead screw 52, ​​so that when the lateral drive motor 51 drives the lateral lead screw 52 to rotate... The transverse connecting block 53 can be driven to slide along the extension direction of the transverse slide rail 603 to cooperate in transferring between different wells of the extraction kit 30 to perform operations such as lysis, washing and elution. The use of a lead screw and slider transmission method can also make the transverse movement more accurate and adapt to the requirements of the extraction scenario. The bottom plate of the extraction module is also equipped with a loading drive motor 41. In order to configure the loading drive mechanism in a limited space, the loading drive motor 41 is also assembled in an inverted manner. Its output is connected to the loading transmission gear 42. The module door 502 is also elastically connected to the bottom plate of the extraction module, and the module door 502 can be fastened to the side wall of the outer shell 100 of the extraction module by means of elasticity.

[0045] Figure 6This is a structural diagram of the cassette loading position 50. The cassette loading position 50 includes a loading platform that can be driven to move. A loading transmission rack 43 is arranged on one side wall of the loading platform, and a cassette limiting member 505 is arranged at the top of the loading platform. The cassette limiting member 505 can accurately and reliably limit the extraction kit 30 transferred thereon within the range of the cassette limiting member 505. In order to achieve higher extraction efficiency, a lysis heating element 503 and an elution heating element 504 are also arranged within the range of the cassette limiting member 505. The lysis hole and elution hole of the extraction kit 30 can contact the corresponding heating element, so that the liquid inside can be heated to the optimal operating temperature, ensuring that the extraction process can be performed efficiently and thoroughly.

[0046] Figure 7 This is a diagram illustrating the process by which the loading position 50 of the box is driven to extend beyond the main body of the extraction module block. Figure 8 This is a process diagram of the loading position 50 receiving the extraction reagent kit 30. Figure 9 This is a diagram illustrating the process of the cassette loading position 50 being driven back into the main body of the extraction module. The entire process requires a loading drive motor 41 to drive the loading transmission gear 42 to rotate. The loading transmission rack 43, meshing with the loading transmission gear 42, can drive the connected loading platform to extend beyond the main body of the extraction module. During the movement, the module compartment door, elastically fastened to the extraction module, can be pushed open by the loading platform, and the rollers 501 at the bottom of the loading platform can slide with low resistance on the module compartment door 502. The cassette loading position 50, after exiting the compartment, can cooperate with the automatic grippers to hold the extraction reagent kit 30, placing it within the range defined by the cassette limiting member 505. This allows the lysis heating element 503 to adhere to the lysis well of the extraction reagent kit 30, and the elution heating element 504 to adhere to the elution well of the extraction reagent kit 30. Then, the loading drive motor 41 rotates in the opposite direction, driving the loading platform back into the extraction module. After the force of the loading platform is removed, the module compartment door 502, under the action of elastic force, can fasten to the storage opening on the outer shell 100.

[0047] Figure 10 This is a diagram illustrating the process by which the magnetic rod loading unit 20 is driven to take up the loaded magnetic rod within the extraction kit 30. Figure 11This is a diagram illustrating the process of the magnetic rod sleeve loading unit 20 loading the magnetic rod sleeve. The extraction kit 30 in this design is also equipped with a magnetic rod sleeve receiving hole, within which multiple magnetic rod sleeves can be arranged side-by-side, simplifying the configuration of the extraction kit 30. The second substrate drive motor 21 can drive the second lifting substrate 23 to descend, thereby causing the first lifting substrate 13 to descend synchronously, capturing the magnetic rod sleeve within the magnetic rod sleeve receiving hole, connecting the magnetic rod sleeve to the end of the magnetic rod sleeve loading unit 20. After loading the magnetic rod sleeve, the second substrate drive motor 21 rotates in the opposite direction to synchronously lift the first and second lifting substrates 13 and 23. After loading the magnetic rod sleeve, the horizontal drive motor 51... The vertical substrate 60 can be moved laterally to above the magnetic bead storage hole of the extraction kit 30. Then, the second substrate drive motor 21 can be lowered so that the magnetic rod sleeve is at least partially submerged in the magnetic bead storage solution. The first substrate drive motor 11 can also rotate to lower the first lifting substrate 13. The distance between the first lifting substrate 13 and the second lifting substrate 23 becomes smaller, and the magnetic rod unit 10 is inserted more into the magnetic rod sleeve, so that the bottom area of ​​the magnetic rod sleeve has a strong magnetic field. Then, the magnetic beads in the magnetic bead storage hole can be magnetically attracted to the outer wall surface of the bottom area of ​​the magnetic rod sleeve. Then, the magnetic beads are transferred to the lysis hole of the extraction kit 30. The transfer process is similar to the position change process after the magnetic rod sleeve is taken in.

[0048] Figure 12 The image shows the lysis state of the loaded magnetic rod sleeve within the lysis well of the extraction kit 30. After the magnetic beads are transferred into the lysis well of the extraction kit 30, the first substrate drive motor 11 drives the first lifting substrate 13 to move upward along the vertical slide rail 601. The distance between the first lifting substrate 13 and the second lifting substrate 23 increases, and more of the magnetic rod unit 10 is removed from the magnetic rod sleeve, making the magnetic field at the bottom of the magnetic rod sleeve weaker or even demagnetized. Then, the rotation drive motor 31 drives the magnetic rod sleeve to rotate, using centrifugal force to quickly and evenly disperse the previously adsorbed magnetic beads into the lysis well. Then, while maintaining a large distance between the first lifting substrate 13 and the second lifting substrate 23, the magnetic rod sleeve can be driven to perform rotational, lifting, or lateral movements, or any combination of complex movements. The lysis heating element 503 at the bottom can perform heating at a set temperature, thus enabling efficient and rapid lysis operations within the lysis well of the extraction kit 30.

[0049] Figure 13The image shows the elution process in the elution wells of the extraction kit 30 using a loaded magnetic rod sleeve. After lysis, the magnetic rod unit 10 and the magnetic rod sleeve loading unit 20 work together to transfer the magnetic beads bound to nucleic acid fragments into the washing wells for washing. There can be two or three washing wells for more thorough washing and purification. After washing, the magnetic beads bound to nucleic acid fragments are adsorbed and collected by the wall of the magnetic rod sleeve and further transferred into the elution wells. To ensure that the nucleic acid bound to the magnetic beads is fully eluted, the elution process includes a mixing step. In the mixing step, the bottom area of ​​the magnetic rod sleeve is in a weak magnetic or demagnetized state. The magnetic rod sleeve rotates, lifts, or a combination of both to ensure thorough mixing. The elution heating element 504 can output a preset heating temperature to make the elution process more efficient. The liquid in the elution wells can be heated to a temperature greater than room temperature but not exceeding 65°C. The optimal temperature range is 30-65°C to ensure elution efficiency and qualified fragment length. After elution, the magnetic rod unit 10 can be inserted more into the magnetic rod sleeve, so that the outer wall surface of the bottom area of ​​the magnetic rod sleeve has strong magnetic properties, adsorbing and collecting the used magnetic beads, which are then recycled back to the previous magnetic bead storage hole.

[0050] Figure 14 This is an overall diagram of the extraction module housed within the outer casing 100. The extraction module can be configured within the cubic space shown in the diagram. The outer casing 100, composed of thin-shell components, can be made of non-metallic materials such as plastic or metallic materials such as steel. A storage opening is provided on one side of the outer casing 100, and a module compartment door 502 is elastically fastened to the storage opening to keep the extraction module in a closed state, thereby reducing the possibility of contamination to the system containing it. More preferably, a fan assembly 70 is also provided on the top of the extraction module. The fan assembly 70 is configured to have its air intake at the top of the extraction module to form a top suction method, which also helps to form a local rotational flow within the module. A filter 701 is connected to the end of its air outlet. This filter can be a HEPA filter with a high efficiency filtration level, so that the air inside the extraction module that may have a risk of contamination is efficiently filtered before being discharged, thus reducing the risk of contamination.

[0051] This document uses specific embodiments to illustrate the principles and implementation methods of this utility model. The descriptions of the embodiments above are only for the purpose of helping to understand the method and core ideas of this utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made to this utility model without departing from the principles of this utility model, and these improvements and modifications also fall within the protection scope of the claims of this utility model.

[0052] In the description of this utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the utility model product is in use. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0053] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

Claims

1. An extraction module, comprising an extraction module body, characterized in that, The extraction module body includes a first lifting base plate, a first base plate drive motor, a first base plate transmission screw, a second lifting base plate, a vertical slide rail, and a vertical base plate. A magnetic rod unit is connected to the first lifting base plate, and a magnetic rod sleeve loading unit is connected to the second lifting base plate. The magnetic rod sleeve loading unit is hollow, and one end can be connected to a magnetic rod sleeve. The magnetic rod unit can pass through the magnetic rod sleeve loading unit and extend into the magnetic rod sleeve. The vertical slide rail is connected to the vertical base plate. The first and second lifting base plates are movably connected to the vertical slide rail via sliders, and the first lifting base plate is always positioned at a higher position relative to the second lifting base plate. The first base plate drive motor is mounted on the second lifting base plate and connected to the first lifting base plate via the first base plate transmission screw. The first base plate drive motor can drive the first base plate to move relative to the second base plate along the vertical slide rail, changing the relative distance between the first lifting base plate and the second base plate.

2. The extraction module as described in claim 1, characterized in that, It also includes a second substrate drive motor and a second substrate transmission screw. The second substrate drive motor is mounted on the vertical substrate and connected to the second lifting substrate through the second substrate transmission screw. The second substrate drive motor can drive the first substrate and the second substrate to move synchronously along the vertical slide rail. The first substrate transmission screw and the second substrate transmission screw are arranged in opposite directions in an inverted manner.

3. The extraction module as described in claim 1, characterized in that, The extraction module also includes a horizontal driving mechanism connected to the vertical base plate and capable of driving the vertical base plate to move horizontally. The horizontal driving mechanism includes a horizontal driving motor, a horizontal lead screw, a horizontal slide rail, a horizontal connecting block, and a horizontal slider. The horizontal driving motor is connected to the horizontal lead screw, and the horizontal lead screw is threadedly connected to the horizontal connecting block. The bottom end of the horizontal connecting block is connected to the horizontal slider, and the top end is connected to the vertical base plate. The horizontal slider is connected to the horizontal slide rail arranged parallel to the horizontal lead screw, and the horizontal slider can move along the extension direction of the horizontal slide rail under the drive of the horizontal driving motor.

4. The extraction module as described in claim 3, characterized in that, The extraction module body also includes a box loading position, which includes a loading platform that can move horizontally and enter and exit the extraction module body, and a loading drive mechanism that can drive the loading platform to move. The top of the loading platform is equipped with a box limiting member, which can limit the extraction reagent kit inside.

5. The extraction module as described in claim 4, characterized in that, The loading drive mechanism includes a loading drive motor, a loading transmission gear, and a loading transmission rack. The loading drive motor is connected to the loading transmission gear, and the loading transmission rack is located on the side wall of the loading platform. The loading transmission gear meshes with the loading transmission rack.

6. The extraction module as described in claim 5, characterized in that, The extraction module is equipped with a thin-shell outer shell, which has a storage opening and a module compartment door that is elastically fastened to the storage opening. The loading platform has rollers at the bottom. When the loading platform moves outward from the outer shell, the module compartment door can be pushed open by the loading platform, and the rollers at the bottom of the loading platform can slide on the module compartment door with low resistance. When the loading platform retracts into the outer shell, the module compartment door can be fastened to the storage opening on the outer shell under the action of elastic force.

7. The extraction module as described in claim 6, characterized in that, A fan assembly is provided on the top of the housing, and a filter element is provided at the air outlet of the fan assembly.

8. The extraction module as described in claim 4, characterized in that, The box is also equipped with a pyrolysis heating element and an elution heating element within its defined range.

9. The extraction module as described in claim 5, characterized in that, The transverse lead screw is arranged along the Y-axis, the loading transmission rack is arranged along the X-axis, and the drive shaft of the loading drive motor is inverted downwards and connected to the loading transmission gear.

10. The extraction module according to any one of claims 1-9, characterized in that, The second lifting base plate is also equipped with a rotary drive mechanism, which includes a rotary drive motor, a rotary drive gear, an intermediate gear and a rotary transmission gear. The rotary transmission gear is located at the end of the magnetic rod sleeve loading unit. Several magnetic rod sleeve loading units are arranged side by side, and the rotary transmission gears corresponding to adjacent magnetic rod sleeve loading units mesh with each other. The rotary drive gear is meshed with the intermediate gear, and the intermediate gear is meshed with any rotary transmission gear.

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