Microbial agent efficient expanding culture device for macrobrachium breeding

By designing a microbial inoculant propagation device for prawn seedling cultivation, which includes components such as a temperature-regulating water tank, an aeration mechanism, and a stirring device, the problems of insufficient temperature, dissolved oxygen, and pH regulation were solved, thus improving propagation efficiency and quality.

CN224186154UActive Publication Date: 2026-05-01GUANGXI GRAIN & OIL SCI RES INST CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
GUANGXI GRAIN & OIL SCI RES INST CO LTD
Filing Date
2025-05-22
Publication Date
2026-05-01

AI Technical Summary

Technical Problem

Existing high-efficiency propagation devices for microbial agents used in prawn breeding lack the function of regulating propagation temperature, dissolved oxygen, and pH, making operation inconvenient.

Method used

A device was designed that includes components such as a temperature-controlled water tank, a culture tank, a stirring mechanism, an aeration mechanism, and a dosing mechanism. The temperature is adjusted by the temperature-controlled water tank, the dissolved oxygen is increased by the aeration mechanism, nutrients are added by the dosing mechanism, the bacterial solution is stirred by the stirring mechanism, and the controller performs the overall operation.

Benefits of technology

It enables precise control of propagation temperature, dissolved oxygen, and pH, thereby improving the propagation efficiency and quality of microbial agents.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to the technical field of microbial agent expanding culture, and discloses a microbial agent efficient expanding culture device for macrobrachium breeding. According to the utility model, the cabinet body; the temperature adjusting water tank is mounted on the inner wall of the bottom of the cabinet body; the expanding culture tank is arranged on the temperature adjusting water tank; the partition plate is fixedly arranged on the inner wall of the cabinet body; the adding mechanism is mounted on the partition plate and is used for adding a carbon source, a nitrogen source and trace elements into the expanding culture tank; the aeration mechanism is mounted on the cabinet body and is used for increasing dissolved oxygen of the bacterial liquid in the propagation tank; the temperature adjusting mechanism is arranged on the temperature adjusting water tank and is used for adjusting the propagation temperature; and the stirring mechanism is arranged at the bottom of the expanding culture tank and is used for stirring the bacterial liquid.
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Description

A high-efficiency propagation device for microbial inoculants used in prawn seedling cultivation Technical Field

[0001] This utility model relates to the field of microbial agent propagation technology, and in particular to a high-efficiency propagation device for microbial agents used in prawn seedling cultivation. Background Technology

[0002] Microbial inoculant propagation is a highly technical and demanding task. Its core objective is to achieve efficient proliferation of inoculants by optimizing culture conditions, while strictly controlling aseptic operation, environmental parameters, and process flow.

[0003] However, existing high-efficiency propagation devices for microbial agents used in prawn breeding often lack the function of regulating propagation temperature, dissolved oxygen, and pH, which is inconvenient. Summary of the Invention

[0004] To address the technical problem that existing high-efficiency propagation devices for microbial agents used in prawn breeding often lack the function of regulating propagation temperature, dissolved oxygen, and pH, this utility model provides a high-efficiency propagation device for microbial agents used in prawn breeding.

[0005] This utility model is achieved using the following technical solution: a high-efficiency microbial inoculant propagation device for prawn seedling cultivation, comprising: a cabinet; a temperature-regulating water tank installed on the inner wall of the bottom of the cabinet; a propagation tank installed on the temperature-regulating water tank; a partition fixedly installed on the inner wall of the cabinet; an addition mechanism installed on the partition for adding carbon source, nitrogen source and trace elements into the propagation tank; an aeration mechanism installed on the cabinet for increasing the dissolved oxygen in the bacterial solution in the propagation tank; a temperature regulating mechanism installed on the temperature-regulating water tank for adjusting the propagation temperature; a stirring mechanism located at the bottom of the propagation tank for stirring the bacterial solution; and a light control device installed on the inner wall of the top of the propagation tank.

[0006] Through the above technical solution, the water in the temperature-regulating water tank can be used to adjust the culture temperature of the bacterial solution in the expansion tank, the expansion tank can be used to expand the microbial agent, the addition mechanism can add carbon source, nitrogen source and trace elements to the microbial solution in the expansion tank, the aeration mechanism can diffuse air into the bacterial solution in the expansion tank, thereby increasing the dissolved oxygen content of the bacterial solution, the temperature regulation mechanism can regulate the water temperature in the temperature-regulating water tank, and the stirring mechanism can stir the bacterial solution in the expansion tank.

[0007] As a further improvement to the above solution, the feeding mechanism includes: multiple storage tanks installed on the partition; multiple discharge pipes respectively installed at the bottom of the multiple storage tanks and connected to the expansion tank; and multiple material valves respectively installed on the multiple discharge pipes.

[0008] Through the above technical solution, carbon source, nitrogen source and trace elements can be added to the microbial liquid in the expansion tank through multiple storage tanks, multiple feeding pipes and multiple feeding valves.

[0009] As a further improvement to the above solution, the aeration mechanism includes: an aeration pipe installed on the inner wall of the expansion tank and extending to the outside of the cabinet; a plurality of aeration holes opened on the aeration pipe; and an air pump installed on one side of the cabinet and connected to the aeration pipe.

[0010] The above technical solution allows for monitoring of dissolved oxygen content in the bacterial solution within the expansion tank using a dissolved oxygen sensor. When the dissolved oxygen content in the bacterial solution is lower than a preset threshold, an air pump is activated. The air pump diffuses air into the bacterial solution within the expansion tank through an aeration pipe and multiple aeration holes, thereby increasing the dissolved oxygen content of the bacterial solution.

[0011] As a further improvement to the above solution, the temperature control mechanism includes: an electric heating tube installed on the inner wall of the temperature-controlled water tank; a chiller installed on one side of the cabinet; a first circulation pipe and a second circulation pipe installed on the chiller and connected to the temperature-controlled water tank; and a water temperature sensor installed on the inner wall of the temperature-controlled water tank.

[0012] Through the above technical solution, the water in the temperature-controlled water tank can be used to adjust the culture temperature of the bacterial solution in the culture tank. The water temperature sensor can monitor the water temperature in the temperature-controlled water tank. When the water temperature in the temperature-controlled water tank is lower than the preset threshold, the controller controls the start of the electric heating tube, which can raise the water temperature in the culture tank. When the water temperature in the culture tank is higher than the preset threshold, the controller controls the start of the chiller, which cools the water in the temperature-controlled water tank through the first circulation pipe and the second circulation pipe.

[0013] As a further improvement to the above solution, the stirring mechanism includes: a stirring rod rotatably mounted on the inner wall of the bottom of the expansion tank; a housing fixedly mounted on the bottom of the expansion tank; and a drive motor fixedly mounted on the inner wall of the housing and connected to the stirring rod.

[0014] The above technical solution uses a drive motor to rotate a stirring rod, which in turn stirs the bacterial solution in the culture tank.

[0015] As a further improvement to the above solution, a liquid extraction pipe is installed on the expansion tank, one end of which extends to the outside of the cabinet, and a liquid extraction valve is provided on the liquid extraction pipe.

[0016] The above technical solution allows for the collection of cultured bacterial solution via a collection tube and a collection valve.

[0017] As a further improvement to the above solution, a dissolved oxygen sensor and a pH sensor are installed on the inner wall of the expansion tank, and an exhaust pipe is installed on the expansion tank, with one end of the exhaust pipe extending to the outside of the cabinet.

[0018] The above technical solution allows for the monitoring of dissolved oxygen content in the bacterial culture tank using a dissolved oxygen sensor, and the monitoring of pH in the bacterial culture tank using a pH sensor.

[0019] As a further improvement to the above solution, a cabinet door is hinged to the cabinet body, and a controller is installed on the cabinet door.

[0020] The device can be operated and controlled by the controller through the above technical solution.

[0021] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0022] This invention allows for the adjustment of the culture temperature of the bacterial solution in the expansion tank via a temperature-controlled water tank. The expansion tank facilitates the expansion of microbial agents. A dosing mechanism adds carbon, nitrogen, and trace elements to the bacterial solution within the expansion tank. An aeration mechanism diffuses air into the bacterial solution, increasing its dissolved oxygen content. A temperature-controlled mechanism regulates the water temperature in the tank, and a stirring mechanism agitates the bacterial solution. Multiple storage tanks, feeding pipes, and valves allow for the addition of carbon, nitrogen, and trace elements. A dissolved oxygen sensor monitors the dissolved oxygen content of the bacterial solution. When the dissolved oxygen content falls below a preset threshold, an air pump is activated. The air pump diffuses air into the bacterial solution through aeration pipes and multiple aeration holes. The system is designed to increase the dissolved oxygen content of the bacterial solution. The temperature of the bacterial solution in the expansion tank can be adjusted by the water in the temperature-controlled water tank. A water temperature sensor monitors the water temperature in the temperature-controlled water tank. When the water temperature in the temperature-controlled water tank is lower than a preset threshold, the controller activates the electric heating element to raise the water temperature in the expansion tank. When the water temperature in the expansion tank is higher than the preset threshold, the controller activates the chiller. The chiller cools the water in the temperature-controlled water tank through the first and second circulation pipes. A drive motor rotates the stirring rod, which agitates the bacterial solution in the expansion tank. The expanded bacterial solution is collected through a dispensing pipe and a dispensing valve. A dissolved oxygen sensor monitors the dissolved oxygen content of the bacterial solution in the expansion tank, and a pH sensor monitors the pH of the bacterial solution. The controller allows for operation and control of the device. Attached Figure Description

[0023] Figure 1 is a schematic diagram of the overall structure of the high-efficiency propagation device for microbial inoculants for prawn seedling cultivation provided in Embodiment 1 of this utility model.

[0024] Figure 2 is a schematic diagram of the front sectional view of the structure in Figure 1;

[0025] Figure 3 is an enlarged structural diagram of point A in Figure 2.

[0026] Explanation of key symbols:

[0027] 1. Cabinet; 2. Temperature-controlled water tank; 3. Propagation tank; 4. Partition; 5. Storage tank; 6. Feed pipe; 7. Feed valve; 8. Aeration pipe; 9. Aeration hole; 10. Air pump; 11. Electric heating element; 12. Chiller; 13. First circulation pipe; 14. Second circulation pipe; 15. Water temperature sensor; 16. Stirring rod; 17. Housing; 18. Drive motor; 19. Liquid extraction pipe; 20. Liquid extraction valve; 21. Dissolved oxygen sensor; 22. pH sensor; 23. Exhaust pipe; 24. Cabinet door; 25. Controller; 26. Light control device. Detailed Implementation

[0028] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0029] Example:

[0030] Referring to Figures 1-3, the high-efficiency propagation device for microbial agents used in the breeding of freshwater prawns in this embodiment includes: a cabinet 1; a temperature-regulating water tank 2 installed on the inner wall of the bottom of the cabinet 1; a propagation tank 3 installed on the temperature-regulating water tank 2; a partition 4 fixedly installed on the inner wall of the cabinet 1; a dosing mechanism installed on the partition 4 for adding carbon source, nitrogen source and trace elements into the propagation tank 3; an aeration mechanism installed on the cabinet 1 for increasing the dissolved oxygen in the bacterial solution in the propagation tank 3; a temperature regulating mechanism installed on the temperature-regulating water tank 2 for adjusting the propagation temperature; a stirring mechanism located at the bottom of the propagation tank 3 for stirring the bacterial solution; and a light control device 26 installed on the inner wall of the top of the propagation tank 3.

[0031] The feeding mechanism includes: multiple storage tanks 5 installed on the partition plate 4; multiple discharge pipes 6 respectively installed at the bottom of the multiple storage tanks 5 and connected to the expansion tank 3; and multiple material valves 7 respectively installed on the multiple discharge pipes 6.

[0032] The aeration mechanism includes: an aeration pipe 8 installed on the inner wall of the expansion tank 3 and extending to the outside of the cabinet 1; a plurality of aeration holes 9 opened on the aeration pipe 8; and an air pump 10 installed on one side of the cabinet 1 and connected to the aeration pipe 8, wherein the air pump 10 is a HIBLOW HP-20.

[0033] The temperature control mechanism includes: an electric heating tube 11 installed on the inner wall of the temperature control water tank 2; a chiller 12 installed on one side of the cabinet 1; a first circulation pipe 13 and a second circulation pipe 14 installed on the chiller 12 and connected to the temperature control water tank 2; and a water temperature sensor 15 installed on the inner wall of the temperature control water tank 2, wherein the water temperature sensor 15 is model TS-01.

[0034] The stirring mechanism includes: a stirring rod 16 rotatably mounted on the inner wall of the bottom of the expansion tank 3; a housing 17 fixedly mounted on the bottom of the expansion tank 3; and a drive motor 18 fixedly mounted on the inner wall of the housing 17 and connected to the stirring rod 16. The model of the drive motor 18 is HTYPG90S-8.

[0035] The expansion tank 3 is equipped with a liquid extraction pipe 19, one end of which extends to the outside of the cabinet 1, and a liquid extraction valve 20 is provided on the liquid extraction pipe 19.

[0036] The inner wall of the expansion tank 3 is equipped with a dissolved oxygen sensor 21 and a pH sensor 22. The expansion tank 3 is equipped with an exhaust pipe 23, one end of which extends to the outside of the cabinet 1. The dissolved oxygen sensor 21 is a HachLDO101 and the pH sensor 2 is a METTLER TOLEDO InPro3250i.

[0037] The cabinet body 1 is hinged with a cabinet door 24, and a controller 25 is installed on the cabinet door 24. The controller 25 is model ZG-ASLM.

[0038] The implementation principle of the high-efficiency propagation device for microbial inoculants used in the breeding of freshwater prawns in this embodiment is as follows:

[0039] Microbial culture solution is added to expansion tank 3. Carbon source, nitrogen source and trace elements can be added to microbial culture solution in expansion tank 3 through multiple storage tanks 5, multiple feeding pipes 6 and multiple feeding valves 7. Drive motor 18 drives stirring rod 16 to rotate, and stirring rod 16 can stir microbial culture solution in expansion tank 3. Water in temperature-controlled water tank 2 can be used to adjust the expansion temperature of microbial culture solution in expansion tank 3. Water temperature sensor 15 can monitor water temperature in temperature-controlled water tank 2. When water temperature in temperature-controlled water tank 2 is lower than preset threshold, controller 25 controls the start of electric heating tube 11, which can raise water temperature in expansion tank 2. When water temperature in expansion tank 2 is higher than preset threshold, controller 25 controls the start of chiller 12, which cools water in temperature-controlled water tank 2 through first circulation pipe 13 and second circulation pipe 14.

[0040] The dissolved oxygen sensor 21 can monitor the dissolved oxygen content of the bacterial solution in the expansion tank 3. When the dissolved oxygen content of the bacterial solution in the expansion tank 3 is lower than the preset threshold, the air pump 10 is started. The air pump 10 can diffuse air into the bacterial solution in the expansion tank 3 through the aeration pipe 8 and multiple aeration holes 9, thereby increasing the dissolved oxygen content of the bacterial solution. The expanded bacterial solution is then taken out through the liquid taking pipe 19 and the liquid taking valve 20.

[0041] The above embodiments are merely preferred embodiments of this utility model and should not be construed as limiting the scope of protection of this utility model. Any non-substantial changes and substitutions made by those skilled in the art based on this utility model shall fall within the scope of protection claimed by this utility model.

Claims

1. A high-efficiency propagation device for microbial inoculants used in prawn seedling cultivation, characterized in that, include: Cabinet body; temperature-controlled water tank installed on the inner wall of the bottom of the cabinet body; The expansion tank is installed on the temperature-controlled water tank; a partition is fixedly installed on the inner wall of the cabinet; a dosing mechanism installed on the partition for adding carbon source, nitrogen source and trace elements into the expansion tank; an aeration mechanism installed on the cabinet for increasing the dissolved oxygen in the bacterial solution in the expansion tank; a temperature control mechanism installed on the temperature-controlled water tank for adjusting the expansion temperature; a stirring mechanism located at the bottom of the expansion tank for stirring the bacterial solution; and a light control device installed on the inner wall of the top of the expansion tank.

2. The high-efficiency propagation device for microbial inoculants used in prawn seedling cultivation as described in claim 1, characterized in that, The feeding mechanism includes: multiple storage tanks installed on the partition; multiple discharge pipes installed at the bottom of the multiple storage tanks and connected to the expansion tank; and multiple material valves installed on the multiple discharge pipes.

3. The high-efficiency propagation device for microbial inoculants used in prawn seedling cultivation as described in claim 1, characterized in that, The aeration mechanism includes: an aeration pipe installed on the inner wall of the expansion tank and extending to the outside of the cabinet; a plurality of aeration holes opened on the aeration pipe; and an air pump installed on one side of the cabinet and connected to the aeration pipe.

4. The high-efficiency propagation device for microbial inoculants used in prawn seedling cultivation as described in claim 1, characterized in that, The temperature control mechanism includes: an electric heating tube installed on the inner wall of the temperature-controlled water tank; a chiller installed on one side of the cabinet; a first circulation pipe and a second circulation pipe installed on the chiller and connected to the temperature-controlled water tank; and a water temperature sensor installed on the inner wall of the temperature-controlled water tank.

5. The high-efficiency propagation device for microbial inoculants used in prawn seedling cultivation as described in claim 1, characterized in that, The stirring mechanism includes: a stirring rod rotatably mounted on the inner wall of the bottom of the expansion tank; a housing fixedly mounted on the bottom of the expansion tank; and a drive motor fixedly mounted on the inner wall of the housing and connected to the stirring rod.

6. The high-efficiency propagation device for microbial inoculants used in prawn seedling cultivation as described in claim 1, characterized in that, The expansion tank is equipped with a liquid extraction pipe, one end of which extends to the outside of the cabinet, and a liquid extraction valve is provided on the liquid extraction pipe.

7. The high-efficiency propagation device for microbial inoculants used in prawn seedling cultivation as described in claim 1, characterized in that, The inner wall of the expansion tank is equipped with a dissolved oxygen sensor and a pH sensor. The expansion tank is equipped with an exhaust pipe, one end of which extends to the outside of the cabinet.

8. The high-efficiency propagation device for microbial inoculants used in prawn seedling cultivation as described in claim 1, characterized in that, The cabinet body is hinged with a cabinet door, and the cabinet door is equipped with a controller.