Self-adaptive feeding cell culture device

Abstract

The utility model discloses a self-adaptive feeding cell culture device, belongs to the related technical field of cell culture, and aims to solve the problems that in the prior art, generally, an operator needs to suck added feeding materials into a rubber head dropper firstly and then drop the feeding materials into a cell culture dish, but when various feeding materials need to be added to cells, the operation is inconvenient, and the cost is low. The utility model discloses a cell culture device, which solves the problem that the working effect of the cell culture device is reduced due to the fact that an operator needs to collect supplemented materials for multiple times and then drip the supplemented materials, the operator cannot conveniently supplement the cells in time, and the cell culture device comprises a box body, the outer wall of the box body is rotatably connected with a knob through a bearing, and the lower end of the knob is fixedly connected with a turntable; and the lower end of the rotating disc communicates with a plurality of storage pipes, the lower ends of the multiple baffles are fixedly connected with one ends of the corresponding springs correspondingly, the other ends of the multiple springs are fixedly connected with the corresponding fixing plates correspondingly, and the outer walls of the multiple fixing plates are fixedly connected with the corresponding round pipes correspondingly.

Description

Technical Field

[0001] This invention belongs to the field of cell culture technology, and more specifically, relates to an adaptive feeding cell culture device. Background Technology

[0002] Cell culture is a technique that simulates the in vivo environment outside the body, enabling cells to survive, grow, multiply, and maintain their main structures and functions. During culture, cells need to be provided with nutrients regularly to promote their growth.

[0003] Current cell culture devices typically require operators to first draw the feed into a dropper before adding it to the cell culture dish when cells need to be fed. However, when multiple feeds are required, operators need to collect the feed multiple times before adding it, which makes it inconvenient for operators to feed the cells in a timely manner and reduces the working efficiency of the cell culture device. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides an adaptive feeding cell culture device. This solves the problem that existing technologies generally require operators to first draw the added feed into a dropper and then drip it into the cell culture dish. However, when multiple feeds are needed for the cells, operators need to collect the feed multiple times before dripping it in, which is inconvenient for operators to feed the cells in a timely manner, thus reducing the working efficiency of the cell culture device.

[0005] To achieve the above objectives, this utility model provides the following technical solution: an adaptive feeding cell culture device, comprising a housing, the outer wall of which is rotatably connected to a knob via a bearing, the lower end of which is fixedly connected to a turntable, the lower end of which is connected to multiple storage tubes, the connection areas of which are made of hard rubber, the multiple storage tubes being threadedly connected to the turntable, the lower ends of which are fixedly connected to corresponding round tubes, the other areas of which are made of soft rubber, labels affixed to the outside of which are multiple storage tubes, the inner walls of which are fixedly connected to corresponding washers, the outer walls of which are abutted against corresponding baffles, the lower ends of which are fixedly connected to one end of corresponding springs, the other ends of which are fixedly connected to corresponding fixing plates, and the outer walls of which are fixedly connected to corresponding round tubes.

[0006] As a preferred technical solution of this utility model, a motor is installed on the inner wall of the box, the output shaft of the motor is fixedly connected to the first connecting rod, the end of the first connecting rod is rotatably connected to the second connecting rod through a pin, the end of the second connecting rod is rotatably connected to the lifting plate through a pin, the inner wall of the lifting plate is slidably connected to four columns through a through hole, and the upper and lower ends of the four columns are fixedly connected to the box.

[0007] As a preferred embodiment of this invention, a cell culture dish is placed above the lifting plate.

[0008] As a preferred embodiment of this utility model, a temperature sensor is installed on the lower inner wall of the box, and a heating block is installed on the upper inner wall of the box.

[0009] As a preferred embodiment of this utility model, the upper end of the box is provided with a feed inlet, the size of which matches the size of the opening of the storage tube on the turntable.

[0010] As a preferred embodiment of this utility model, the outer wall of the box is equipped with two sliding doors.

[0011] This invention provides an adaptive fed cell culture device, which has the following beneficial effects:

[0012] 1. This new device comprises a housing, knob, turntable, storage tube, round tube, washer, baffle, spring, and fixing plate. When it is necessary to add supplementary material to the cell culture dish, the operator turns the knob, which drives the turntable to rotate. The turntable then drives the storage tube below to rotate. When the storage tube containing the required supplementary material rotates to the top of the cell culture dish, the operator stops turning the knob, opens the sliding door, and presses the storage tube. The supplementary material in the storage tube moves downward, pushing open the baffle. At this time, the spring is compressed, and a gap appears between the washer and the baffle, allowing the supplementary material to fall into the cell culture dish. After the supplementary material is added, the operator releases the storage tube, and the spring returns to its original position, pressing the baffle against the washer to prevent the supplementary material from falling further. This method allows for quick location and addition of the required supplementary material when needed, avoiding the need for multiple collections and additions when multiple supplementary materials are required, thus improving the working efficiency of the cell culture device.

[0013] 2. The device utilizes a motor, a first connecting rod, a second connecting rod, a lifting plate, and cell culture dishes. When replenishment is needed, the first motor drives the first connecting rod to rotate, which in turn drives the second connecting rod to rotate. The second connecting rod then lifts the lifting plate, which in turn lifts the cell culture dishes placed above it. This brings the cell culture dishes closer to the circular tube, reducing splashing caused by falling replenishment and improving the working efficiency of the cell culture device. Attached Figure Description

[0014] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0015] Figure 2 for Figure 1 A frontal view of the sliding door after it is opened;

[0016] Figure 3 for Figure 2 A diagram showing a view from the left, looking upwards;

[0017] Figure 4 for Figure 3 A cross-sectional view of the central circular tube.

[0018] In the diagram: 1. Box body; 2. Knob; 3. Turntable; 4. Storage tube; 5. Round tube; 6. Washer; 7. Baffle; 8. Spring; 9. Fixing plate; 10. Motor; 11. First connecting rod; 12. Second connecting rod; 13. Lifting plate; 14. Cell culture dish; 15. Column; 16. Temperature sensor; 17. Heating block; 18. Sliding door; 19. Feed inlet. Detailed Implementation

[0019] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0020] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In addition, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0021] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0022] Please see Figures 1 to 4This utility model provides a technical solution for an adaptive fed cell culture device: It includes a housing 1, the outer wall of which is rotatably connected to a knob 2 via bearings. Multiple wiring holes are machined on the surface of the housing 1, allowing wires from a motor 10, a temperature sensor 16, and a heating block 17 to be connected to the outside of the housing 1. The knob 2 rotates on the housing 1, and its lower end is fixedly connected to a turntable 3, causing the turntable 3 to rotate. The lower end of the turntable 3 is connected to multiple storage tubes 4, which in turn rotate the storage tubes 4. The connection areas between the storage tubes 4 and the turntable 3 are made of hard rubber. All storage tubes 4 are threadedly connected to the turntable 3 and can be removed from the turntable 3 for cleaning. The lower ends of each storage tube 4 are fixedly connected to corresponding round tubes 5, which move the round tubes 5. The remaining areas of the storage tubes 4 are made of soft rubber, which facilitates operation. Pressing the button causes labels to be affixed to the outside of multiple storage tubes 4, making it easy for operators to identify nutrients. The inner walls of multiple round tubes 5 are fixedly connected to corresponding washers 6. Washers 6 prevent gaps between baffles 7 and round tubes 5. The outer walls of multiple washers 6 are pressed against corresponding baffles 7. The lower ends of multiple baffles 7 are fixedly connected to one end of corresponding springs 8. Springs 8 always keep baffles 7 pressed against washers 6. The other ends of multiple springs 8 are fixedly connected to corresponding fixing plates 9. Springs 8 are fixed on fixing plates 9. The outer walls of multiple fixing plates 9 are fixedly connected to corresponding round tubes 5. When it is necessary to add feed to the cell culture dish 14, the operator turns knob 2, which drives turntable 3 to rotate. Turntable 3 then drives storage tube 4 below to rotate. When storage tube 4, which contains the feed to be added, rotates above the cell culture dish 14, the operator stops turning knob 2, then opens sliding door 18 and presses storage tube 4. The feed in storage tube 4 moves downward and pushes open baffle 7. At this time, spring 8 is pressed, and a gap appears between washer 6 and baffle 7, allowing the feed to fall into the cell culture dish 14. After the feed is added, the operator releases storage tube 4, and spring 8 returns to its original position, pressing baffle 7 against washer 6 to prevent the feed from falling further. This method allows for quick retrieval and addition of the required feed when needed, avoiding the need for multiple collections and additions when multiple feeds are required, thus improving the working efficiency of the cell culture device.

[0023] The inner wall of the housing 1 is equipped with a motor 10. The model of the motor 10 is selected according to actual needs, and only needs to meet the working requirements are required. The output shaft of the motor 10 is fixedly connected to the first connecting rod 11. The motor 10 drives the first connecting rod 11 to rotate. The end of the first connecting rod 11 is rotatably connected to the second connecting rod 12 through a pin. The first connecting rod 11 drives the second connecting rod 12 to rotate. The end of the second connecting rod 12 is rotatably connected to the lifting plate 13 through a pin. The second connecting rod 12 drives the lifting plate 13 to move. The inner wall of the lifting plate 13 is slidably connected to four columns 15 through through holes. The lifting plate 13 moves along the columns 15. The upper and lower ends of the four columns 15 are fixedly connected to the housing 1. The columns 15 are fixed on the housing 1.

[0024] Cell culture dish 14 is placed on top of lifting plate 13.

[0025] A temperature sensor 16 is installed on the lower inner wall of the chamber 1, which can detect the temperature inside the chamber 1. A heating block 17 is installed on the upper inner wall of the chamber 1, which can regulate the temperature inside the chamber 1.

[0026] The upper end of the box 1 is provided with a feed inlet 19, which feeds nutrients into the storage tube 4. The size of the feed inlet 19 matches the size of the opening of the storage tube 4 on the turntable 3.

[0027] The outer wall of the box 1 is equipped with two sliding doors 18, which insulate the interior of the box 1.

[0028] The specific usage and function of this embodiment are as follows:

[0029] In use, the operator first feeds nutrients into the storage tube 4 through the inlet 19. Then, when nutrients need to be added to the cell culture dish 14, the external power supply to the motor 10 is connected, starting the motor 10. The motor 10 drives the first connecting rod 11 to rotate, which in turn drives the second connecting rod 12 to rotate. The second connecting rod 12 then moves the lifting plate 13, which in turn moves the cell culture dish 14 above it, bringing it to the side of the circular tube 5. Afterward, the motor 10 stops, and the operator turns the knob 2. The knob 2 drives the turntable 3 to rotate, which in turn drives the storage tube 4 below to rotate. When the storage tube 4 contains the required supplementary nutrients... After rotating the knob 2 above the cell culture dish 14, the operator stops rotating the knob 2, then opens the sliding door 18 and presses the storage tube 4. The feed material in the storage tube 4 moves downward and pushes open the baffle 7. At this time, the spring 8 is pressed, and a gap appears between the washer 6 and the baffle 7, allowing the feed material to fall into the cell culture dish 14. After the feed material is added, the operator releases the storage tube 4, and the spring 8 returns to its original position, pressing the baffle 7 against the washer 6 to prevent the feed material from falling further. This method allows for quick retrieval and addition of the required feed material when needed, thus avoiding the need for multiple collections and additions when multiple feed materials are required, thereby improving the working efficiency of the cell culture device.

[0030] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. An adaptive fed-batch cell culture device, characterized in that: The device includes a housing. The outer wall of the housing is rotatably connected to a knob via a bearing. The lower end of the knob is fixedly connected to a turntable. The lower end of the turntable is connected to multiple storage tubes. The connection areas between the multiple storage tubes and the turntable are made of hard rubber. The multiple storage tubes are threadedly connected to the turntable. The lower ends of the multiple storage tubes are fixedly connected to corresponding round tubes. Other areas of the multiple storage tubes are made of soft rubber. Labels are affixed to the outside of the multiple storage tubes. The inner walls of the multiple round tubes are fixedly connected to corresponding washers. The outer walls of the multiple washers are abutted against corresponding baffles. The lower ends of the multiple baffles are fixedly connected to one end of corresponding springs. The other ends of the multiple springs are fixedly connected to corresponding fixing plates. The outer walls of the multiple fixing plates are fixedly connected to corresponding round tubes.

2. The adaptive fed cell culture device according to claim 1, characterized in that: A motor is installed on the inner wall of the housing. The output shaft of the motor is fixedly connected to the first connecting rod. The end of the first connecting rod is rotatably connected to the second connecting rod through a pin. The end of the second connecting rod is rotatably connected to the lifting plate through a pin. The inner wall of the lifting plate is slidably connected to four columns through through holes. The upper and lower ends of the four columns are fixedly connected to the housing.

3. The adaptive fed cell culture device according to claim 2, characterized in that: A cell culture dish is placed above the lifting plate.

4. The adaptive fed cell culture device according to claim 1, characterized in that: A temperature sensor is installed on the lower inner wall of the box, and a heating block is installed on the upper inner wall of the box.

5. The adaptive fed cell culture device according to claim 1, characterized in that: The upper end of the box is provided with a feed inlet, the size of which matches the size of the opening of the storage tube on the turntable.

6. The adaptive fed cell culture device according to claim 1, characterized in that: The outer wall of the box is equipped with two sliding doors.

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