Breathable membrane cell culture bottle

Abstract

The utility model relates to the technical field of cell culture devices, and provides a gas-permeable membrane cell culture bottle, which at least comprises a bottle bottom provided with a first clamping body; the bottle bottom fixing frame is arranged outside the bottle bottom in a sleeving mode, a second clamping body matched with the first clamping body is arranged on the bottle bottom fixing frame, and during assembling, the first clamping body and the second clamping body are clamped with each other so that the bottle bottom can be prevented from falling off from the bottle bottom fixing frame. According to the breathable membrane cell culture bottle, the first clamping body is designed at the bottle bottom, the second clamping body is arranged at the corresponding position of the bottle bottom fixing frame, and during assembly, the first clamping body and the second clamping body are clamped with each other, so that stable connection between the bottle bottom and the bottle bottom fixing frame is ensured, and the bottle bottom is prevented from falling off from the bottle bottom fixing frame.

Description

Technical Field

[0001] This utility model relates to the field of cell culture device technology, specifically to a breathable membrane cell culture flask. Background Technology

[0002] A breathable membrane cell culture flask is a laboratory consumable specifically designed for cell culture. Its bottom utilizes breathable membrane technology to optimize gas exchange and improve cell culture efficiency. These breathable membrane-based cell culture flasks offer significant advantages in improving gas exchange efficiency, reducing contamination risks, and optimizing the cell growth environment, and are widely used in fields such as immunotherapy, stem cell research, and antibody development. A breathable membrane cell culture flask mainly consists of a tall body, a cap with gas-liquid tubing, a breathable membrane base, and several interfaces. In use, cells and sufficient culture medium are added to the flask in one go, and it can then be placed in an incubator for static culture without frequent medium changes. During culture, oxygen and carbon dioxide are automatically exchanged through the bottom breathable membrane. Cells obtain nutrients from the culture medium without the need for shaking or stirring; cells can grow on the breathable membrane. After proliferation is complete, approximately 90% of the cell-free culture medium at the top is removed, and the cells are then collected and processed for reuse.

[0003] The existing permeable membrane cell culture flask mainly includes a cap, a body, a permeable membrane, a bottom, and a bottom fixing frame. The bottom fixing frame has a protruding structure. During assembly, the edge of the bottom is pressed against the inside of the protruding structure. However, the permeable membrane cell culture flask with this assembly structure design has high requirements for injection molding precision, and the bottom is prone to falling off the bottom fixing frame. Utility Model Content

[0004] Therefore, this utility model aims to solve the problem that in the prior art, the permeable membrane cell culture bottle has a protruding structure on the bottle bottom fixing frame, and the bottom edge of the bottle is pressed against the inside of the protruding structure during assembly. The permeable membrane cell culture bottle with this assembly structure design has high requirements for injection molding precision, and the bottle bottom is easy to fall off the bottle bottom fixing frame. Therefore, this utility model provides a permeable membrane cell culture bottle.

[0005] To solve the above-mentioned technical problems, the technical solution of this utility model is as follows:

[0006] This utility model provides a breathable membrane cell culture flask, comprising at least: a flask bottom, on which a first clip is provided; and a flask bottom fixing frame, sleeved on the outside of the flask bottom, wherein a second clip adapted to the first clip is provided on the flask bottom fixing frame, wherein during assembly, the first clip and the second clip engage with each other to prevent the flask bottom from falling off the flask bottom fixing frame.

[0007] Furthermore, the first card body is a raised structure, and the second card body is a hollow groove; and / or, the first card body is a hollow groove, and the second card body is a raised structure.

[0008] Furthermore, when the first card body is a raised structure and the second card body is a hollow groove, the first card body is disposed on the outer side wall of the bottle bottom along the circumferential direction of the bottle bottom, and the hollow groove is disposed on the side wall of the bottle bottom fixing frame along the circumferential direction of the bottle bottom fixing frame; when the first card body is a hollow groove and the second card body is a raised structure, the hollow groove is disposed on the side wall of the bottle bottom along the circumferential direction of the bottle bottom, and the first card body is disposed on the inner side wall of the bottle bottom fixing frame along the circumferential direction of the bottle bottom fixing frame.

[0009] Furthermore, the breathable membrane cell culture flask also includes a body, which at least partially extends into the bottom support frame and is connected to the bottom of the flask.

[0010] Furthermore, the breathable membrane cell culture flask also includes a breathable membrane disposed between the body and the bottom of the flask.

[0011] Furthermore, the breathable membrane cell culture flask also includes a support mesh disposed on the bottom of the flask, and the breathable membrane is disposed on the side of the support mesh facing the body of the flask.

[0012] Furthermore, the outer diameter of the bottle body is equal to the inner diameter of the bottle bottom fixing bracket; a first sealing ring is provided on the outer peripheral edge of the breathable membrane, and the bottom end face of the bottle body is pressed onto the first sealing ring.

[0013] Furthermore, the outer diameter of the bottle body is smaller than the inner diameter of the bottle bottom fixing bracket, and an extension is provided on the bottom outer wall of the bottle body, the outer diameter of the extension being equal to the inner diameter of the bottle bottom fixing bracket; a first sealing ring and a second sealing ring are provided on the outer peripheral edge of the breathable membrane, the bottom end face of the bottle body is pressed against the first sealing ring, and the bottom end face of the extension is pressed against the second sealing ring.

[0014] Furthermore, a protruding pressing part is provided on the bottom end face of the bottle body, and a groove adapted to the pressing part is provided on the side of the first sealing ring facing the bottle body; when the bottom end face of the bottle body is pressed onto the first sealing ring, the pressing part is embedded in the groove.

[0015] Furthermore, the bottle bottom is provided with a plurality of first ventilation openings; the bottle bottom fixing bracket is provided with a plurality of second ventilation openings.

[0016] The technical solution of this utility model has the following advantages:

[0017] The breathable membrane cell culture flask provided by this utility model has a first locking body at the bottom and a second locking body at the corresponding position of the bottom fixing frame. During assembly, the first locking body and the second locking body engage with each other to ensure a stable connection between the bottom of the flask and the bottom fixing frame, thereby preventing the bottom of the flask from falling off the bottom fixing frame. Attached Figure Description

[0018] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the bottom of the breathable membrane cell culture flask and the bottom fixing frame in an embodiment of the present invention;

[0020] Figure 2 This is a schematic diagram of the bottom of the breathable membrane cell culture flask in an embodiment of this utility model;

[0021] Figure 3 This is a schematic diagram of the bottom fixing frame of the breathable membrane cell culture flask in an embodiment of the present invention;

[0022] Figure 4 This is an exploded view of a breathable membrane cell culture flask according to one embodiment of the present invention.

[0023] Figure 5 for Figure 4 A cross-sectional view of a breathable membrane cell culture flask.

[0024] Figure 6 for Figure 5 A magnified schematic diagram of a local structure;

[0025] Figure 7 This is an exploded view of a breathable membrane cell culture flask in another embodiment of the present invention.

[0026] Figure 8 for Figure 7 A cross-sectional view of the body of a breathable membrane cell culture flask.

[0027] Figure 9 for Figure 7 A magnified schematic diagram of a partial cross-sectional structure of a breathable membrane cell culture flask.

[0028] Explanation of reference numerals in the attached figures:

[0029] 1. Bottle bottom; 2. First clamping body; 3. Bottle bottom fixing bracket; 4. Second clamping body; 5. First venting notch; 6. Second venting notch; 7. Bottle body; 8. Ventilation membrane; 9. Support mesh; 10. First sealing ring; 11. Second sealing ring; 12. Extension; 13. Pressing part. Detailed Implementation

[0030] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0031] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0032] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0033] Furthermore, the technical features involved in the different embodiments of this utility model described below can be combined with each other as long as they do not conflict with each other.

[0034] like Figures 1 to 3As shown, this embodiment provides a breathable membrane cell culture flask, comprising at least: a flask bottom 1, which may be a circular plate-shaped structure, with a first retaining body 2 disposed on the flask bottom 1; and a flask bottom fixing frame 3, which may be a circular ring or cylindrical structure, fitted over the flask bottom 1, with a second retaining body 4 disposed on the flask bottom fixing frame 3 that is adapted to the first retaining body 2. During assembly, the first retaining body 2 and the second retaining body 4 engage with each other to prevent the flask bottom 1 from falling off the flask bottom fixing frame 3. The first retaining body 2 and the second retaining body 4 may be in a groove and protrusion configuration, a threaded configuration, a pin and hole configuration, or other structures that allow for a stable connection between the flask bottom 1 and the flask bottom fixing frame 3, etc.

[0035] The breathable membrane cell culture flask provided in this embodiment has a first locking body 2 designed at the bottom 1, and a second locking body 4 opened at the corresponding position of the bottom fixing frame 3. During assembly, the first locking body 2 and the second locking body 4 engage with each other to ensure that the connection between the bottom 1 and the bottom fixing frame 3 is stable, so as to prevent the bottom 1 from falling off the bottom fixing frame 3.

[0036] For example, the first card body 2 is a raised structure, and the second card body 4 is a hollow groove; and / or, the first card body 2 is a hollow groove, and the second card body 4 is a raised structure. The raised structure can be strip-shaped, plate-shaped, block-shaped, or other structures that protrude outwards relative to the bottle bottom 1, etc. The hollow groove can be a through hole, notch, slot, etc., adapted to the shape and size of the raised structure. After the bottle bottom 1 is assembled with the bottle bottom fixing bracket 3, the raised structure is inserted into the hollow groove so that the bottle bottom 1 cannot detach relative to the bottle bottom fixing bracket 3 in the axial direction along the bottle bottom 1.

[0037] In one embodiment, when the first card body 2 is a raised structure and the second card body 4 is a hollow groove, the first card body 2 is disposed on the outer side wall of the bottle bottom 1 along the circumferential direction of the bottle bottom 1, and the hollow groove is disposed on the side wall of the bottle bottom fixing frame 3 along the circumferential direction of the bottle bottom fixing frame 3. For example, multiple raised structures can be evenly spaced on the bottle bottom 1, and correspondingly, multiple hollow grooves can be evenly spaced on the bottle bottom fixing frame 3.

[0038] In one embodiment, when the first card body 2 is a hollow groove and the second card body 4 is a raised structure, the hollow groove is disposed on the side wall of the bottle bottom 1 along the circumferential direction of the bottle bottom 1, and the first card body 2 is disposed on the inner side wall of the bottle bottom fixing frame 3 along the circumferential direction of the bottle bottom fixing frame 3. For example, multiple hollow grooves can be evenly spaced on the bottle bottom 1, and correspondingly, multiple raised structures can be evenly spaced on the bottle bottom fixing frame 3.

[0039] In one embodiment, the bottle bottom 1 is provided with both a hollow groove and a raised structure; correspondingly, the bottle bottom fixing bracket 3 is also provided with both a hollow groove and a raised structure.

[0040] like Figure 4 , Figure 5 As shown, the breathable membrane cell culture flask also includes a body 7, which at least partially extends into the bottom support 3 and is connected to the bottom 1. The body 7 can be a columnar structure with an internal cavity for accommodating the target organism and the required culture medium.

[0041] The cell culture flask also includes a breathable membrane 8, disposed between the flask body 7 and the flask bottom 1. For example, the breathable membrane 8 can be a silicone breathable membrane 8, with a thickness ranging from 0.15mm to 0.25mm, and a height from the breathable membrane 8 to the lower edge of the flask bottom fixing bracket 3 ranging from 1.5cm to 2.5cm. This configuration allows for the free exchange of oxygen and carbon dioxide, eliminating the need for frequent culture medium replacements, supporting high-density cell culture, and reducing the risk of contamination caused by manual operation.

[0042] The breathable membrane cell culture flask also includes a support net 9, which is disposed on the bottom 1 of the flask. The breathable membrane 8 is disposed on the side of the support net 9 facing the body 7 of the flask. The support net 9 can support the breathable membrane 8 above.

[0043] like Figure 6 As shown, in one embodiment, the outer diameter of the bottle body 7 is equal to the inner diameter of the bottle bottom fixing bracket 3; a first sealing ring 10 is provided on the outer peripheral edge of the breathable membrane 8, and the bottom end face of the bottle body 7 is pressed onto the first sealing ring 10. For example, the first sealing ring 10 can be a silicone sealing ring, and the first sealing ring 10 can be an integral structure with the breathable membrane 8. During assembly, the support net 9 is first placed on the bottle bottom 1, the breathable membrane 8 with the first sealing ring 10 is installed on the bottle bottom 1, the bottle bottom fixing bracket 3 is inserted into the bottle body 7 from above, the bottle bottom fixing bracket 3 is aligned with the bottle bottom 1, and the bottle body 7 is pressed down. The protruding structure of the bottle bottom 1 moves relative to the bottle bottom fixing bracket 3, and the protruding structure is inserted into the hollow groove of the bottle bottom fixing bracket 3. The bottle bottom 1 is fixed in position and cannot move, so there is no risk of falling off.

[0044] like Figure 7 , Figure 9As shown, in one embodiment, the outer diameter of the bottle body 7 is smaller than the inner diameter of the bottle bottom fixing bracket 3. An extension 12 is provided on the bottom outer wall of the bottle body 7, and the outer diameter of the extension 12 is equal to the inner diameter of the bottle bottom fixing bracket 3. A first sealing ring 10 and a second sealing ring 11 are concentrically arranged on the outer peripheral edge of the breathable membrane 8. The bottom end face of the bottle body 7 is pressed onto the first sealing ring 10, and the bottom end face of the extension 12 is pressed onto the second sealing ring 11. For example, the extension 12 can be an annular plate-like structure extending radially along the bottle body 7, and the edge of the extension 12 can be provided with a stepped surface to facilitate connection with the bottle bottom fixing bracket 3. For example, both the first sealing ring 10 and the second sealing ring 11 can be silicone sealing rings, and the second sealing ring 11 can be an integral structure with the breathable membrane 8. With this configuration, the extension 12 on the outside of the bottle body 7 is used to widen the bottle body 7 so that its outer diameter is the same as that of the wide diameter bottle body 7. The bottle bottom fixing frame 3, the breathable membrane 8, and the bottle bottom 1 can be shared, reducing the number of molds and manufacturing costs.

[0045] like Figure 8 , Figure 9 As shown, the bottom surface of the bottle body 7 has a protruding pressing portion 13, and the side of the first sealing ring 10 facing the bottle body 7 has a groove adapted to the pressing portion 13. When the bottom surface of the bottle body 7 is pressed onto the first sealing ring 10, the pressing portion 13 is embedded in the groove. For example, the pressing portion 13 can be a dot-shaped, block-shaped, or strip-shaped structure, and the pressing portion 13 can be continuously distributed around the circumference of the bottle body 7. For example, the shape and size of the groove are adapted to the pressing portion 13. With this configuration, the bottom of the bottle body 7 is pressed onto the first sealing ring 10 by the pressing portion 13, preventing external air from entering the interior of the bottle body 7 through the gap between the bottle body 7 and the breathable membrane 8, thereby improving the sealing effect.

[0046] The bottle bottom 1 has several first ventilation holes 5, and the bottle bottom fixing frame 3 has several second ventilation holes 6. For example, several first ventilation holes 5 can be provided on the bottom and side surfaces of the bottle bottom 1, and several second ventilation holes 6 can be provided on the side and bottom surfaces of the bottle bottom fixing frame 3. For example, the first ventilation holes 5 and second ventilation holes 6 can be circular, elliptical, semi-circular, or other irregularly shaped holes or grooves. The number of first ventilation holes 5 and second ventilation holes 6 can be selected as needed, for example, up to 20. This arrangement ensures sufficient gas exchange between the inside and outside of the bottle.

[0047] The permeable membrane cell culture flask in this application should also include a cap, gas-liquid pipelines, and other structures, which are existing technologies known to those skilled in the art and will not be described in detail here.

[0048] In summary, the body 7, the breathable membrane 8, and the bottom 1 of the cell culture flask in this application are pressed together by the bottom fixing bracket 3 to form a more stable connection; the bottom is equipped with a silicone breathable membrane 8, the outer periphery of which is a silicone sealing ring, and a support net 9 is provided between the breathable membrane 8 and the bottom 1, allowing free exchange of oxygen and carbon dioxide, eliminating the need for frequent culture medium replacement, supporting high-density cell culture, and reducing the risk of contamination caused by manual operation.

[0049] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the protection scope of this invention.

Claims

1. A breathable membrane cell culture flask, characterized in that, At least including: Bottle bottom (1), on which a first card body (2) is provided; A bottle bottom fixing bracket (3) is fitted over the bottle bottom (1). The bottle bottom fixing bracket (3) is provided with a second locking body (4) that is compatible with the first locking body (2). During assembly, the first locking body (2) and the second locking body (4) engage with each other to prevent the bottle bottom (1) from falling off the bottle bottom fixing bracket (3).

2. The gas-permeable membrane cell culture flask according to claim 1, characterized in that, The first card body (2) is a raised structure, and the second card body (4) is a hollow groove; And / or, the first card body (2) is a hollow groove, and the second card body (4) is a raised structure.

3. The gas-permeable membrane cell culture flask according to claim 2, characterized in that, When the first card body (2) is a protruding structure and the second card body (4) is a hollow groove, the first card body (2) is arranged on the outer side wall of the bottle bottom (1) along the circumferential direction of the bottle bottom (1), and the hollow groove is arranged on the side wall of the bottle bottom fixing frame (3) along the circumferential direction of the bottle bottom fixing frame (3). When the first card body (2) is a hollow groove and the second card body (4) is a protruding structure, the hollow groove is set on the side wall of the bottle bottom (1) along the circumferential direction of the bottle bottom (1), and the first card body (2) is set on the inner side wall of the bottle bottom fixing frame (3) along the circumferential direction of the bottle bottom fixing frame (3).

4. The gas-permeable membrane cell culture flask according to claim 1, characterized in that, It also includes a bottle body (7), which at least partially extends into the bottle bottom fixing frame (3) and is connected to the bottle bottom (1).

5. The gas-permeable membrane cell culture flask according to claim 4, characterized in that, It also includes a breathable membrane (8) disposed between the bottle body (7) and the bottle bottom (1).

6. The gas-permeable membrane cell culture flask according to claim 5, characterized in that, It also includes a support net (9) disposed on the bottom of the bottle (1), and the breathable membrane (8) is disposed on the side of the support net (9) facing the bottle body (7).

7. The gas-permeable membrane cell culture flask according to claim 5, characterized in that, The outer diameter of the bottle body (7) is equal to the inner diameter of the bottle bottom fixing bracket (3); The outer periphery of the breathable membrane (8) is provided with a first sealing ring (10), and the bottom end face of the bottle body (7) is pressed onto the first sealing ring (10).

8. The gas-permeable membrane cell culture flask according to claim 5, characterized in that, The outer diameter of the bottle body (7) is smaller than the inner diameter of the bottle bottom fixing bracket (3), and an extension (12) is provided on the bottom outer side wall of the bottle body (7), the outer diameter of the extension (12) being equal to the inner diameter of the bottle bottom fixing bracket (3); The outer periphery of the breathable membrane (8) is provided with a first sealing ring (10) and a second sealing ring (11) arranged concentrically. The bottom end face of the bottle body (7) is pressed onto the first sealing ring (10), and the bottom end face of the extension (12) is pressed onto the second sealing ring (11).

9. The gas-permeable membrane cell culture flask according to any one of claims 7 or 8, characterized in that, The bottom surface of the bottle body (7) is provided with a protruding pressing part (13), and the side of the first sealing ring (10) facing the bottle body (7) is provided with a groove that matches the pressing part (13). When the bottom end face of the bottle body (7) is pressed onto the first sealing ring (10), the pressing part (13) is embedded in the groove.

10. The breathable membrane cell culture flask according to claim 1, characterized in that, The bottle bottom (1) is provided with several first ventilation openings (5); The bottle bottom fixing bracket (3) is provided with several second ventilation notches (6).

Images