Photochemical analysis test strip structure

Abstract

The utility model provides a photochemical analysis test strip structure which comprises a test strip body, a sample introduction area, a sample introduction flow channel and a reaction detection area which are sequentially communicated are arranged in the test strip body, and a sample introduction port is formed in the test strip body; a hydrophobic layer is arranged at the bottom of the reaction detection area, a notch and a guide hole which are sequentially communicated are formed in the hydrophobic layer, and the guide hole and the reaction detection area are concentrically arranged; the part, located above the hydrophobic layer, of the reaction detection area is an edge area, the other part is a detection center area, and a reagent is arranged in the detection center area; a plurality of first air holes communicated with the edge area are formed in the test strip body. Through the arrangement of the hydrophobic layer, a sample entering the reaction detection area preferentially fills the detection center area and then diffuses to the edge area, the sample is guided to flow orderly, and bubbles are forced to migrate to the edge area and are discharged from the first air hole in the flowing process of the sample, so that the bubble interference of the detection center area is effectively eliminated, and the detection accuracy is further improved.

Description

Technical Field

[0001] This utility model relates to the field of body fluid analysis technology, and in particular to a photochemical analysis strip structure. Background Technology

[0002] Photochemical analysis strips are rapid diagnostic tools based on optical detection principles. They work by reacting specific chemical reagents loaded on the strips with the target analyte to produce color changes, fluorescence, or chemiluminescence signals. These signals are then combined with optical detection instruments (such as spectrophotometers and fluorescence detectors) to achieve detection and analysis.

[0003] For example, Chinese patent application CN119574538A discloses a blood lipid test card based on photochemical principles, including a card body. The card body has a sample application port, a calibration area, and at least one test area. The sample application port is connected to both the calibration area and the test area. Both the calibration area and the test area have pores for communication with the outside. The calibration area has a reaction membrane and a hemolytic agent, and the test area has an enzyme layer corresponding to the test item and an interference-removing membrane. The card body includes a base plate and a top cover that can be attached together. The base plate has several grooves that correspond to the calibration area and the test area. The top cover has a notch and several flow channels. The notch forms the sample application port. One end of each flow channel is connected to the notch, and the other end of each flow channel is connected to a groove. In the above-mentioned prior art test card, when in use, the sample flows into the test area through the sample application port and flow channels. Air bubbles in the sample in the test area are not easily expelled, affecting the accuracy of the test.

[0004] Therefore, existing technologies still need to be improved and developed. Utility Model Content

[0005] The purpose of this invention is to provide a photochemical analysis strip structure that addresses the shortcomings and deficiencies of existing technologies.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0007] This utility model provides a photochemical analysis test strip structure, including a test strip body, wherein the test strip body is provided with a sample injection area, a sample injection channel and a reaction detection area connected sequentially along the sample flow direction, and the test strip body is provided with a sample inlet connected to the sample injection area;

[0008] The bottom of the reaction detection area is provided with a hydrophobic layer, and the hydrophobic layer has a notch and a guide hole that are connected sequentially along the direction of sample flow. The guide hole is concentrically arranged with the reaction detection area. The part of the reaction detection area above the hydrophobic layer is the edge area, and the rest is the detection center area. The detection center area contains reagents.

[0009] The test strip body is provided with a plurality of first air holes that communicate with the edge region.

[0010] It is understood that the reagents can be attached to the testing center through processes such as freeze drying and screen printing deposition.

[0011] By setting up a hydrophobic layer, the sample entering the reaction detection zone first fills the detection center area before spreading to the edge area, guiding the sample to flow in an orderly manner. During the flow, the sample forces the bubbles to migrate to the edge area and be discharged from the outside through the first pore, thereby effectively eliminating bubble interference in the detection center area and improving the accuracy of the detection.

[0012] According to the above scheme, multiple first pores are evenly distributed above the edge area.

[0013] According to the above scheme, the first vent is configured as a conical orifice, with the side of the first vent with a smaller diameter facing outwards. This configuration helps to increase the speed at which gas is discharged to the outside.

[0014] According to the above scheme, the sample inlet channel is set to a serpentine shape.

[0015] The sample inlet channel connecting the sample inlet area and the reaction detection area is designed as a serpentine shape. The continuous bending design of this serpentine shape will cause frequent changes in the flow direction of the sample entering the sample inlet channel, thereby increasing the speed at which the sample flows into the reaction detection area and promoting the mixing efficiency of reagents and samples.

[0016] According to the above scheme, the outlet of the sample injection area is connected to the first end of the sample injection channel, and the outlet of the sample injection area is configured as a tapered structure facing the sample injection channel.

[0017] The above structural design helps to gradually accelerate the sample as it flows from the outlet of the injection zone into the injection channel, thus allowing the sample to enter the injection channel more smoothly and effectively avoiding the formation of air bubbles.

[0018] According to the above scheme, the cone angle α of the tapered structure is 5-30°. By controlling the cone angle within 5-30°, it is ensured that the sample can obtain sufficient acceleration during outflow, while avoiding the increase in flow resistance caused by an excessively large cone angle.

[0019] According to the above scheme, the test strip body includes a light-transmitting film, a double-sided adhesive layer and a hydrophilic film arranged sequentially from bottom to top, and the hydrophobic layer is disposed between the light-transmitting film and the double-sided adhesive layer;

[0020] The double-sided adhesive layer is provided with a sample inlet, a sample guide groove, and a detection hole connected in sequence, and the hydrophilic membrane is provided with the sample inlet; the sample inlet, the transparent membrane, and the hydrophilic membrane form the sample inlet area, the sample guide groove, the transparent membrane, and the hydrophilic membrane form the sample inlet channel, and the detection hole, the hydrophobic layer, the transparent membrane, and the hydrophilic membrane form the reaction detection area.

[0021] By using a test strip body composed of a light-transmitting film, a hydrophobic layer, a double-sided adhesive layer, and a hydrophilic film stacked from top to bottom, the production process can be simplified.

[0022] According to the above scheme, a superhydrophilic guide band is provided on the bottom surface of the hydrophilic membrane, and the superhydrophilic guide band covers the outlet of the sample inlet and the sample inlet guide groove. Through the above structural arrangement, the sample in the sample inlet area is rapidly flowed into the sample inlet channel under the siphon effect of the sample inlet channel and the action of the superhydrophilic guide band, and then rapidly flows out to the reaction detection area.

[0023] According to the above scheme, the test strip body also includes a cover film disposed on the bottom surface of the light-transmitting membrane, and the cover film has light-transmitting holes corresponding to the detection holes. The cover film provides support, which helps to improve the mechanical properties of the test strip body.

[0024] According to the above scheme, the test strip body also includes a waste liquid storage area, which is connected to and located downstream of the reaction detection area; the test strip body also has a second vent hole connected to the reaction detection area. The waste liquid storage area is used to store the waste liquid after the reaction, preventing backflow from affecting the detection accuracy.

[0025] The beneficial effects of this utility model are as follows:

[0026] This invention features a hydrophobic layer at the bottom of the reaction detection zone. By using this hydrophobic layer, the sample entering the reaction detection zone first fills the detection center area before spreading to the edge area, guiding the sample to flow in an orderly manner. During the flow, the sample forces the bubbles to migrate to the edge area and be discharged from the outside through the first pore, thereby effectively eliminating bubble interference in the detection center area and improving the accuracy of the detection. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the structure of this utility model;

[0028] Figure 2 This is an exploded structural diagram of the present invention;

[0029] Figure 3 This is a schematic diagram of the structure of the hydrophobic layer described in this utility model;

[0030] Figure 4 This is a schematic diagram of the structure of the double-sided adhesive layer described in this utility model;

[0031] Figure 5 This is a schematic diagram of the structure of the hydrophilic membrane described in this utility model.

[0032] In the diagram: 1. Test strip body; 11. Sample injection area; 111. Tapered structure; 12. Sample injection channel; 13. Reaction detection area; 131. Detection center area; 132. Edge area; 14. Waste liquid storage area; 2. Cover film; 21. Light-transmitting hole; 3. Light-transmitting film; 4. Hydrophobic layer; 41. Notch; 42. Guide hole; 5. Double-sided adhesive layer; 51. Sample injection hole; 52. Sample injection guide groove; 53. Detection hole; 6. Hydrophilic film; 61. Sample inlet; 62. First pore; 63. Superhydrophilic guide band; 64. Second pore. Detailed Implementation

[0033] The technical solution of this utility model will be described below with reference to the accompanying drawings and embodiments.

[0034] like Figure 1-5 As shown, this utility model provides a photochemical analysis test strip structure, including a test strip body 1. The test strip body 1 has a sample injection area 11, a sample injection channel 12, and a reaction detection area 13 that are sequentially connected along the sample flow direction. The test strip body 1 has a sample inlet 61 that communicates with the sample injection area 11. The bottom of the reaction detection area 13 has a hydrophobic layer 4. The hydrophobic layer 4 has a notch 41 and a guide hole 42 that are sequentially connected along the sample flow direction. The guide hole 42 is concentrically arranged with the reaction detection area 13. The part of the reaction detection area 13 above the hydrophobic layer 4 is an edge area 132, and the remaining part is a detection center area 131. The detection center area 131 contains reagents. The test strip body 1 has a plurality of first pores 62 that communicate with the edge area 132.

[0035] This embodiment takes the detection of hemoglobin as an example. The reagent contains a hemolytic agent and is attached to the detection center area 131 by a freeze-drying process.

[0036] By setting the hydrophobic layer 4, the sample entering the reaction detection zone 13 first fills the detection center zone 131 before spreading to the edge zone 132, guiding the sample to flow in an orderly manner. During the flow, the sample forces the bubbles to migrate to the edge zone 132 and be discharged from the outside through the first pore 62, thereby effectively eliminating the bubble interference in the detection center zone 131 and improving the accuracy of the detection.

[0037] Furthermore, a plurality of the first pores 62 are evenly distributed above the edge region 132.

[0038] Furthermore, the first vent 62 is configured as a tapered vent, with the side of the first vent 62 with a smaller diameter facing outwards. This configuration helps to increase the speed at which gas is discharged to the outside.

[0039] Furthermore, the sample inlet channel 12 is configured as a serpentine shape.

[0040] The sample inlet channel 12 connecting the sample inlet area 11 and the reaction detection area 13 is set as a serpentine shape. The continuous bending design of this serpentine shape will cause frequent changes in the flow direction of the sample entering the sample inlet channel 12, thereby increasing the speed at which the sample flows into the reaction detection area 13 and promoting the mixing efficiency of reagents and samples.

[0041] Furthermore, the outlet of the sample inlet area 11 is connected to the first end of the sample inlet channel 12, and the outlet of the sample inlet area 11 is configured as a tapered structure 111 facing the sample inlet channel 12.

[0042] The above structural design helps to gradually accelerate the sample as it flows from the outlet of the injection zone 11 into the injection channel 12, thereby allowing the sample to enter the injection channel 12 more smoothly and effectively avoiding the formation of air bubbles.

[0043] Furthermore, the cone angle α of the tapered structure 111 is 5-30°. By controlling the cone angle α within 5-30°, it is ensured that the sample can obtain sufficient acceleration during outflow, while avoiding the increase in flow resistance caused by an excessively large cone angle.

[0044] Furthermore, the test strip body 1 includes a light-transmitting membrane 3, a double-sided adhesive layer 5, and a hydrophilic membrane 6 arranged sequentially from bottom to top. The hydrophobic layer 4 is disposed between the light-transmitting membrane 3 and the double-sided adhesive layer 5. The double-sided adhesive layer 5 is provided with a sample inlet 51, a sample guide groove 52, and a detection hole 53 connected in sequence. The hydrophilic membrane 6 is provided with the sample inlet 61. The sample inlet 51, the light-transmitting membrane 3, and the hydrophilic membrane 6 form the sample inlet area 11. The sample guide groove 52, the light-transmitting membrane 3, and the hydrophilic membrane 6 form the sample inlet channel 12. The detection hole 53, the hydrophobic layer 4, the light-transmitting membrane 3, and the hydrophilic membrane 6 form the reaction detection area 13.

[0045] In this embodiment, a hydrophobic ink is applied to the light-transmitting film 3 using a screen printing method, thereby forming a hydrophobic layer 4 with a notch 41 and a guide hole 42 on the light-transmitting film 3. By using a test strip body 1 composed of a light-transmitting film 3, a hydrophobic layer 4, a double-sided adhesive layer 5, and a hydrophilic film 6 in a layered structure from top to bottom, the production process is simplified.

[0046] Furthermore, a superhydrophilic guide band 63 is provided on the bottom surface of the hydrophilic membrane 6, which covers the outlet of the sample inlet 51 and the sample inlet guide groove 52. Through this structural arrangement, the sample in the sample inlet area 11 flows rapidly into the sample inlet channel 12 under the siphon effect of the sample inlet channel 12 and the action of the superhydrophilic guide band 63, and then rapidly flows out into the reaction detection area 13.

[0047] Furthermore, the test strip body 1 also includes a cover film 2 disposed on the bottom surface of the light-transmitting film 3, and the cover film 2 has a light-transmitting hole 21 corresponding to the detection hole 53. By providing the cover film 2, a supporting function is provided, which is beneficial to improving the mechanical properties of the test strip body 1.

[0048] Furthermore, the test strip body 1 is also provided with a waste liquid storage area 14, which is connected to and located downstream of the reaction detection area 13; the test strip body 1 is provided with a second vent 64 that is connected to the reaction detection area 13. The waste liquid storage area 14 is used to store the waste liquid after the reaction to prevent backflow from affecting the detection accuracy.

[0049] In use, the photochemical analysis strip structure of this invention involves adding the sample into the injection area 11 through the injection port 61. Under the siphon effect of the injection channel 12 and the action of the superhydrophilic guide band 63, the sample rapidly enters the injection channel 12 from the outlet of the injection area 11. After being accelerated by the continuous bending design of the injection channel 12, the sample quickly enters the reaction detection area 13 and dissolves and mixes with the reagent. The sample entering the reaction detection area 13, under the hydrophobic effect of the hydrophobic layer 4, preferentially fills the detection center area 131 and then diffuses to the edge area 132, guiding the orderly flow of the sample. During the flow, the sample forces the bubbles to migrate to the edge area 132 and exit from the outside through the first pore 62. The sample reacts with the reagent in the reaction detection area 13, and the red blood cells in the sample are destroyed to release hemoglobin. The waste liquid after the reaction flows into the waste liquid storage area 14, and the gas in the waste liquid storage area 14 is discharged from the outside through the second pore 64. Then, the strip body 1 is placed into an optical detection instrument to irradiate and detect the sample in the detection center area 131.

[0050] The above description is only a preferred embodiment of the present utility model. Therefore, all equivalent changes or modifications made to the structure, features and principles described in the claims of the present utility model patent application are included in the scope of the present utility model patent application.

Claims

1. A photochemical analysis strip structure, comprising a strip body (1), wherein the strip body (1) is provided with an injection zone (11), an injection channel (12), and a reaction detection zone (13) sequentially connected along the direction of sample flow, and the strip body (1) is provided with an injection port (61) connected to the injection zone (11), characterized in that, The bottom of the reaction detection area (13) is provided with a hydrophobic layer (4). The hydrophobic layer (4) has a notch (41) and a guide hole (42) connected sequentially along the direction of sample flow. The guide hole (42) is concentrically arranged with the reaction detection area (13). The part of the reaction detection area (13) above the hydrophobic layer (4) is the edge area (132), and the rest is the detection center area (131). The detection center area (131) contains reagents. The test strip body (1) is provided with a plurality of first air holes (62) that communicate with the edge area (132).

2. The photochemical analysis strip structure according to claim 1, characterized in that, Multiple first pores (62) are evenly distributed above the edge region (132).

3. The photochemical analysis strip structure according to claim 2, characterized in that, The first vent (62) is configured as a tapered vent, with the side of the first vent (62) with a smaller diameter facing outward.

4. The photochemical analysis strip structure according to claim 1, characterized in that, The sample inlet channel (12) is configured as a serpentine shape.

5. The photochemical analysis strip structure according to claim 1, characterized in that, The outlet of the injection zone (11) is connected to the first end of the injection channel (12), and the outlet of the injection zone (11) is configured as a tapered structure (111) facing the injection channel (12).

6. The photochemical analysis strip structure according to claim 5, characterized in that, The cone angle α of the tapered structure (111) is 5-30°.

7. The photochemical analysis strip structure according to any one of claims 1-6, characterized in that, The test strip body (1) includes a light-transmitting film (3), a double-sided adhesive layer (5) and a hydrophilic film (6) arranged sequentially from bottom to top, and the hydrophobic layer (4) is disposed between the light-transmitting film (3) and the double-sided adhesive layer (5); The double-sided adhesive layer (5) is provided with a sample inlet (51), a sample guide groove (52) and a detection hole (53) connected in sequence, and the hydrophilic membrane (6) is provided with the sample inlet (61); The sample inlet (51), the transparent membrane (3) and the hydrophilic membrane (6) form the sample inlet area (11), the sample inlet guide groove (52), the transparent membrane (3) and the hydrophilic membrane (6) form the sample inlet channel (12), and the detection hole (53), the hydrophobic layer (4), the transparent membrane (3) and the hydrophilic membrane (6) form the reaction detection area (13).

8. The photochemical analysis strip structure according to claim 7, characterized in that, The bottom surface of the hydrophilic membrane (6) is provided with a superhydrophilic guide band (63), which covers the outlet of the injection port (51) and the injection guide groove (52).

9. The photochemical analysis strip structure according to claim 7, characterized in that, The test strip body (1) also includes a cover film (2) disposed on the bottom surface of the light-transmitting film (3), and the cover film (2) has a light-transmitting hole (21) corresponding to the detection hole (53).

10. The structure of the photochemical analysis strip according to claim 1, characterized in that, The test strip body (1) is also provided with a waste liquid storage area (14), which is connected to the reaction detection area (13) and located downstream of the reaction detection area (13); the test strip body (1) is provided with a second vent (64) that is connected to the reaction detection area (13).

Images