Tissue culture container

By designing a multi-layered folded tissue culture container assembly and a rotatable disk structure, the problems of limited culture capacity and inconvenient operation of existing containers have been solved, enabling efficient and convenient observation and cultivation of multiple cultures.

CN224205885UActive Publication Date: 2026-05-08YANBIAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
YANBIAN UNIV
Filing Date
2024-03-05
Publication Date
2026-05-08

AI Technical Summary

Technical Problem

Existing tissue culture containers have a limited capacity for culture, low efficiency, and cannot simultaneously observe multiple cultures, and are inconvenient to operate.

Method used

A multi-layered folded tissue culture container assembly and a rotatable disk structure were designed. The rotatable disk assembly, which includes multiple placement holes, can simultaneously hold multiple culture containers. Stable rotation observation is ensured by magnetic pads and limiting pivots. Labels are attached for easy identification, and a threaded rotating structure achieves container sealing and isolation.

Benefits of technology

It improves the yield and operational efficiency of culture containers, allows simultaneous observation of multiple cultures, and is easy to rotate, with easy-to-identify labels, good sealing, and simple assembly.

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Abstract

The utility model relates to the technical field of tissue culture containers, and discloses a tissue culture container which comprises a plurality of placing hole rotatable disc assemblies, and a plurality of multi-layer folding tissue culture container assemblies are placed on the tops of the placing hole rotatable disc assemblies in an annular mode. The multi-layer folding tissue culture device is reasonable in structure, the eight culture container placing holes are annularly formed in the top surface of the rotatable disc assembly through the placing holes, eight multi-layer folding tissue culture container assemblies can be placed in the multi-layer folding tissue culture device at the same time, the yield and efficiency are improved, a plurality of cultured tissues can be cultured and observed at the same time, and the culture efficiency is improved. The whole device can be rotated leftwards and rightwards by using a rotating disc, a limiting rotating shaft and an arc-shaped handle at the bottom, so that different culture containers can be watched in the same visual field, in addition, the multi-layer folding tissue culture container assembly is formed by vertically folding three culture containers, three tissues can be cultured at the same time, and the multi-layer folding tissue culture container assembly is convenient to assemble.
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Description

Technical Field

[0001] This utility model relates to the field of tissue culture container technology, specifically a tissue culture container. Background Technology

[0002] Plant tissue culture, or tissue culture for short, is one of the important forms of agricultural modernization. It is increasingly widely used in agricultural production and plays an irreplaceable role in the cultivation of horticultural plants, medicinal herbs, and rare plants, especially in the cultivation of tissue culture seedlings of medicinal Dendrobium officinale, Notopterygium incisum, strawberry, virus-free potato, sugarcane, grape, banana, and various flowers.

[0003] Tissue culture flasks are the culture containers for plant tissue growth. They are the most important and widely used production equipment in industrialized tissue culture, affecting the efficiency and quality of tissue culture production. Currently, tissue culture flasks used in industrialized production are generally divided into two types: Erlenmeyer flasks and cylindrical flasks. Erlenmeyer flasks have a large body and a small mouth, while cylindrical flasks have an enlarged mouth. Both types of tissue culture flasks are relatively tall.

[0004] When culturing existing embryonic tissues, they are usually cultured in petri dishes. However, the number of petri dishes that can be cultured is limited. Using only one culture container to culture one substance reduces efficiency and makes it impossible to rotate the container. Experimenters cannot directly operate on other parts of the petri dish, and it is also easy to bump into the container during operation, making it very inconvenient to use.

[0005] A plant tissue culture container disclosed in Chinese utility model patent application publication CN211510154U, although made of polypropylene material for the body, cover and separator ring, is inexpensive, the overall culture capacity is limited, the efficiency is low, it can only culture one substance at a time, and the storage and observation structure is lacking. Utility Model Content

[0006] (a) Technical problems to be solved

[0007] To address the shortcomings of existing technologies, this invention provides a tissue culture container that solves the problems of limited overall culture capacity, low efficiency, the ability to culture only one type of substance at a time, and a lack of storage and observation structures.

[0008] (II) Technical Solution

[0009] To achieve the above objectives, this utility model provides the following technical solution: a tissue culture container comprising multiple rotatable disc assemblies with placement holes, wherein several multi-layered folded tissue culture container assemblies are arranged in a ring shape on the top of the multiple rotatable disc assemblies with placement holes. Each multi-layered folded tissue culture container assembly includes a culture container A, the bottom of which is covered by a magnetic base. A thread A is formed on the interior of the culture container A near its top edge. The top of the culture container A is covered by a culture container B, the top surface of which has a threaded ring A, the bottom surface of which has a threaded ring B. The top of the culture container B is covered by a culture container C, the interior of which has threads B near its top edge and bottom surface, respectively. The top of the culture container C is covered by a culture container sealing cap, the bottom surface of which has a threaded ring C.

[0010] Optionally, the plurality of rotatable disc assemblies with placement holes includes a disc base, and a rotary adjustment nut is connected to the center of the top surface of the disc base.

[0011] Optionally, the circular base has several label openings in a ring shape on its four sides, and several culture container placement holes in a ring shape on its top surface.

[0012] Optionally, the interior of all the culture container placement holes is covered with a magnetic pad, and a threaded rotating rod passes through the center of the disc base.

[0013] Optionally, a turntable is connected to the bottom of the threaded rod, and the surface of the turntable is provided with several connecting holes in an annular shape.

[0014] Optionally, a limiting shaft passes through the interior of all the connecting holes, and an arc-shaped handle is connected to the bottom of all the limiting shafts.

[0015] Optionally, the number of the multi-layer folded tissue culture container assemblies is eight, and all the multi-layer folded tissue culture container assemblies are placed in a ring on the top surface of the multiple placement hole rotatable disc assemblies.

[0016] In summary, the technical effects and advantages of this utility model are as follows:

[0017] 1. This utility model has a reasonable structure. The top surface of the rotatable disc assembly with multiple placement holes has eight culture container placement holes in a ring shape. Eight multi-layer folded tissue culture container assemblies can be placed inside at the same time, which improves the yield and efficiency. Multiple tissues can be cultured and observed at the same time. The bottom turntable, limiting shaft and arc handle can be used to rotate the entire device left and right to view different culture containers in the same field of view. In addition, the multi-layer folded tissue culture container assembly is composed of three culture containers folded up and down, which can culture three tissues at the same time, and it is easy to assemble.

[0018] 2. In this utility model, the top surface of the rotatable disc assembly with multiple placement holes has eight culture container placement holes in a ring shape. Each culture container placement hole is equipped with a magnetic pad, and a magnetic base is also provided at the bottom of the culture container. They can attract each other and are not easy to fall or shake during rotation. Labeling openings are provided around the placement holes to indicate which tissue is placed inside the corresponding culture container. Finally, the turntable can be rotated to rotate the threaded rod. Rotating the thread inside the rotating adjusting nut in one direction will rotate the top disc base upward, and rotating it in the other direction will rotate the top disc base downward. Holding the arc-shaped handle directly drives the entire top device to rotate without moving up and down. The limiting pivot restricts the arc-shaped handle to extend outward by 90°, so that the top device can be rotated without being hindered by the wobbling of the arc-shaped handle.

[0019] 3. In this utility model, the multi-layer folded tissue culture container assembly has a total of three culture containers, namely culture container A, culture container B and culture container C. A culture container sealing cap is placed on the top of culture container C to seal culture container C. The two containers below are sealed and isolated by a structure that can be installed by screw rotation, and are made of a material that is used for bacterial isolation. The number of folds of culture containers B and C can be customized according to the height limit. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of this utility model;

[0021] Figure 2 Schematic diagram of the extended rotating structure of a rotatable disk assembly with multiple placement holes;

[0022] Figure 3 A schematic diagram of the combined structure of a multi-layered folded tissue culture container assembly;

[0023] Figure 4 An exploded structural diagram of a rotatable disk assembly with multiple placement holes;

[0024] Figure 5 This is a schematic diagram of the exploded structure of a multi-layered folded tissue culture container assembly.

[0025] In the diagram: 1. Rotatable disc assembly with multiple placement holes; 101. Disc base; 102. Rotary adjusting nut; 103. Labeling port; 104. Culture container placement hole; 105. Magnetic pad; 106. Threaded rotating rod; 107. Turntable; 108. Connecting hole; 109. Limiting rotating shaft; 110. Arc-shaped handle; 2. Multi-layer folded tissue culture container assembly; 201. Culture container A; 202. Magnetic base; 203. Thread A; 204. Culture container B; 205. Threaded ring A; 206. Threaded ring B; 207. Culture container C; 208. Thread B; 209. Culture container sealing cap; 210. Threaded ring C. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] Example: Reference Figure 1 , Figure 2 and Figure 4The illustrated tissue culture container includes a rotatable disc assembly 1 with multiple placement holes. Several multi-layered folded tissue culture container assemblies 2 are arranged in a ring shape on the top of the rotatable disc assembly 1. The rotatable disc assembly 1 includes a disc base 101. A rotating adjusting nut 102 is connected to the center of the top surface of the disc base 101. Several label slots 103 are arranged in a ring shape on the surrounding surface of the disc base 101. Several culture container placement holes 104 are arranged in a ring shape on the top surface of the disc base 101. Each culture container placement hole 104 has a magnetic pad 105 inside. A magnetic base 202 is also provided at the bottom of the culture container to prevent it from falling or shaking during rotation. Label slots 103 are provided around the placement holes for attaching labels. The corresponding culture container indicates which tissue is placed inside. The inside of the culture container placement hole 104 is covered with a magnetic pad 105. A threaded rotating rod 106 passes through the center of the disc base 101. The bottom of the threaded rotating rod 106 is connected to a turntable 107. The surface of the turntable 107 has several connecting holes 108 in a ring shape. The inside of the connecting holes 108 passes through a limiting rotating shaft 109. The bottom of all the limiting rotating shafts 109 is connected to an arc-shaped handle 110. There are eight multi-layer folded tissue culture container assemblies 2. All the multi-layer folded tissue culture container assemblies 2 are placed in a ring shape on the top surface of the rotatable disc assembly 1 with multiple placement holes. The bottom turntable 107, limiting rotating shafts 109 and arc-shaped handles 110 can be used to rotate the entire device left and right to view different culture containers in the same field of view.

[0028] like Figure 3 and Figure 5 As shown, in this embodiment, the multi-layer folded tissue culture container assembly 2 includes a culture container A201, the bottom of which is covered by a magnetic base 202. A thread A203 is formed on the interior of the culture container A201 near its top edge. A culture container B204 covers the top of the culture container A201. A threaded ring A205 is formed on the top surface of the culture container B204, and a threaded ring B206 is formed on the bottom surface of the culture container B204. A culture container C207 covers the top of the culture container B204. The interior of the culture container C207 near its top edge... The edge and bottom surface are respectively provided with threads B208. The top of the culture container C207 is covered with a culture container sealing cap 209. The bottom surface of the culture container sealing cap 209 is provided with a threaded ring C210. ​​The culture container sealing cap 209 is placed on the top of the culture container C207 to seal the culture container C207. The two containers below are sealed and isolated by a structure that can be installed by rotating the thread, and are made of a material that is used for bacterial isolation. The culture containers B204 and C207 can be customized in terms of the number of folds according to the height limit.

[0029] The working principle of this practical application is as follows:

[0030] Firstly, the top surface of the rotatable disc assembly 1, with multiple placement holes, has eight culture container placement holes 104 arranged in a ring shape. This allows for the simultaneous placement of eight multi-layered folded tissue culture container assemblies 2, improving yield and efficiency. Multiple tissues can be cultured and observed simultaneously. The entire device can be rotated left and right using the bottom turntable 107, limiting shaft 109, and arc-shaped handle 110, enabling viewing of different culture containers from the same perspective. Furthermore, the multi-layered folded tissue culture container assembly 2 consists of three culture containers folded vertically, allowing for the simultaneous culture of three tissues and facilitating assembly. The top surface of the rotatable disc assembly 1 has eight culture container placement holes 104 arranged in a ring shape. Each placement hole 104 has a magnetic pad 105 inside, and a magnetic base 202 is also provided at the bottom of the culture container to prevent it from falling or shaking during rotation. Labeling slots 103 are provided around the placement holes to indicate the type of tissue placed inside each culture container. Finally... The turntable 107 can be rotated to rotate the threaded rod 106. Rotating the internal thread of the rotating adjusting nut 102 in one direction will rotate the top disc base 101 upward, and rotating it in the other direction will rotate the top disc base 101 downward. Holding the arc handle 110 directly will drive the entire top device to rotate, without moving it up or down. The limiting shaft 109 restricts the arc handle 110 to extend outward by 90°, preventing the top device from being unable to rotate due to the arc handle 110 shaking. Finally, through the multi-layer folded tissue culture container assembly 2 structure, there are a total of three culture containers, namely culture container A201, culture container B204 and culture container C207. A culture container sealing cap 209 is placed on the top of culture container C207 to seal it. The two containers below are sealed and isolated by a structure that can be installed by rotating the thread, and are made of a bacterial isolation material. The number of folds of culture containers B204 and C207 can be customized according to the height limit.

[0031] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A tissue culture container comprising a plurality of rotatable disk assemblies with placement holes (1), characterized in that: The top of the multiple placement hole rotatable disk assembly (1) has several multi-layer folded tissue culture container assemblies (2) arranged in a ring shape; The multi-layer folded tissue culture container assembly (2) includes a culture container A (201), the bottom of which is covered by a magnetic base (202). A thread A (203) is provided inside the culture container A (201) near the top edge. A culture container B (204) is covered on the top of the culture container A (201). A threaded ring A (205) is provided on the top surface of the culture container B (204). A threaded ring B (206) is provided on the bottom surface of the culture container B (204). A culture container C (207) is covered on the top of the culture container B (207). A threaded ring B (208) is provided inside the culture container C (207) near the top edge and on the bottom surface, respectively. A culture container sealing cap (209) is covered on the top of the culture container C (207). A threaded ring C (210) is provided on the bottom surface of the culture container sealing cap (209).

2. The tissue culture container according to claim 1, characterized in that: The plurality of mounting holes rotatable disc assembly (1) includes a disc base (101), and a rotating adjusting nut (102) is connected to the center of the top surface of the disc base (101).

3. A tissue culture container according to claim 2, characterized in that: The circular base (101) has several label openings (103) in a ring shape on its four sides, and several culture container placement holes (104) in a ring shape on its top surface.

4. A tissue culture container according to claim 3, characterized in that: The interior of all the culture container placement holes (104) is covered with magnetic pads (105), and a threaded rotating rod (106) passes through the center of the disc base (101).

5. A tissue culture container according to claim 4, characterized in that: The bottom of the threaded rotating rod (106) is connected to a turntable (107), and the surface of the turntable (107) is provided with several connecting holes (108) in an annular shape.

6. A tissue culture container according to claim 5, characterized in that: All of the connecting holes (108) have a limiting pivot (109) running through their interiors, and all of the limiting pivots (109) have an arc-shaped handle (110) connected to their bottoms.

7. A tissue culture container according to claim 1, characterized in that: The number of the multi-layer folded tissue culture container assembly (2) is eight, and all the multi-layer folded tissue culture container assemblies (2) are placed in a ring on the top surface of the multiple placement hole rotatable disc assemblies (1).

Citation Information

Patent Citations

  • Plant tissue culture container

    CN211510154U