Polypeptide extraction device

Abstract

The utility model relates to the technical field of polypeptide extraction, and discloses a polypeptide extraction device which comprises an extractor body, a feeding port formed in the top surface of the extractor body, a limiting ring attached to the outer side is arranged on the top surface of the extractor body, and an inlet closing assembly covering the feeding port is arranged in the limiting ring. And a detachable raw material freezing and thawing assembly is arranged on the limiting ring. The inlet closing assembly is arranged at the feed port of the extractor body, so that the feed port is accurately sealed, and pollution caused when the raw material freezing and thawing assembly is mounted and dismounted is avoided; the raw material freezing and thawing assembly is arranged at the feeding port, polypeptide raw materials can be subjected to freezing and thawing treatment and directly fed into the extractor body to be extracted, the pollution phenomenon is avoided, and daily maintenance is facilitated through the raw material freezing and thawing assembly which can be quickly disassembled.

Description

Technical Field

[0001] This utility model relates to the field of polypeptide extraction technology, specifically to a polypeptide extraction device. Background Technology

[0002] Polypeptides are compounds formed by 2-50 amino acids linked by peptide bonds. They are widely involved in physiological processes such as signal transduction, immune regulation, and enzyme catalysis in organisms. Polypeptide extraction is mainly achieved through natural extraction, chemical synthesis, and gene recombination. Strict temperature control is required during extraction, such as low-temperature cell disruption and appropriate temperature enzymatic hydrolysis or synthesis, to avoid peptide denaturation. Chromatographic separation and freeze-drying techniques are also used to ensure purity and activity, ultimately leading to applications in medicine, food, and cosmetics.

[0003] Peptide extraction requires freeze-thaw treatment of the sample during the cell disruption stage. This involves repeatedly freezing and thawing the cells within a temperature range of -20°C to 37°C to disrupt the cell membrane structure, release intracellular peptides, and prevent peptide denaturation due to high temperatures. Current peptide extraction methods separate the freeze-thaw treatment from the extraction process, and then transfer the sample using other tools after the freeze-thaw treatment. This operation is complex, and the sample may be exposed to room temperature or high temperatures during transfer, leading to peptide denaturation or degradation. It may also come into contact with environmental microorganisms or impurities, introducing contamination and reducing peptide purity. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a polypeptide extraction device that can freeze-thaw polypeptide raw materials and directly feed them into the extractor body for extraction, thus avoiding contamination.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a polypeptide extraction device, comprising an extractor body, an inlet provided on the top surface of the extractor body, a limiting ring provided on the top surface of the extractor body to fit the outer side, an inlet closure component covering the inlet provided inside the limiting ring, and a detachable raw material freeze-thaw component provided on the limiting ring.

[0006] Furthermore, the inlet closure assembly includes electric telescopic rods disposed on both sides of the inner wall of the limiting ring. The ends of the electric telescopic rods are connected to closing plates. The symmetrically arranged closing plates extend and retract under the drive of the electric telescopic rods. When closed, they fit together to cover the feed inlet. The top surface of the extractor body is provided with limiting strips that fit both sides of the closing plates.

[0007] Furthermore, the raw material freeze-thaw assembly includes a freeze-thaw shell attached to the top surface of the limiting ring, a limiting block attached to the outside of the limiting strip on the bottom surface of the freeze-thaw shell, a feeding valve pipe located downwards in the middle of the bottom surface of the freeze-thaw shell, the feeding valve pipe being placed inside the feeding port, a freeze-thaw chamber fixedly connected to the feeding valve pipe inside the freeze-thaw shell, the lower end of the freeze-thaw chamber being frustoconical and the upper end being cylindrical, a heating platform attached to the outer side of the lower end of the freeze-thaw chamber, a cooling annular plate attached to the outer side of the upper end of the freeze-thaw chamber, temperature sensors on the inner walls of both the upper and lower ends of the freeze-thaw chamber, six sets of temperature sensors evenly arranged circumferentially on the inner wall of the freeze-thaw chamber, an adding port leading to the freeze-thaw chamber on one side of the top surface of the freeze-thaw shell, and a temperature display panel connected to the temperature sensors located next to the adding port.

[0008] Furthermore, an observation window is provided on the top surface of the freeze-thaw shell on the symmetrical side of the filling port.

[0009] Furthermore, the bottom surface of the freeze-thaw shell is symmetrically provided with a fixing block that fits against the inner wall of the limiting ring, and the fixing block and the limiting ring are fixedly connected by a pin.

[0010] Furthermore, a stirring motor is installed in the middle of the top surface of the freeze-thaw chamber. The drive shaft of the stirring motor is connected to a stirring rod, which is installed inside the freeze-thaw chamber. The stirring rod at the upper end of the freeze-thaw chamber is equipped with arc-shaped stirring blades, and the stirring rod at the lower end of the freeze-thaw chamber is equipped with scraper-type stirring blades that fit against the inner wall of the freeze-thaw chamber.

[0011] Furthermore, control boxes are provided on both sides of the top surface of the freeze-thaw chamber, and a pressure stirrer is provided on the bottom surface of the control box, which is attached to the inner wall of the upper end of the freeze-thaw chamber. The pressure stirrer and the arc-shaped stirring blades are kept in non-contact.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] By setting an inlet closure component at the feed port of the extractor body, the feed port is precisely sealed, avoiding contamination during the installation and disassembly of the raw material freeze-thaw assembly. By setting the raw material freeze-thaw assembly at the feed port, the peptide raw material can be freeze-thawed and directly fed into the extractor body for extraction, avoiding contamination. The quick-release raw material freeze-thaw assembly facilitates daily maintenance.

[0014] With the cooperation of the heating platform and the cooling ring plate, the peptide raw materials can be heated and frozen in a cycle to achieve freeze-thaw operation. Six sets of temperature sensors are arranged circumferentially on the freeze-thaw chamber to achieve three-dimensional modeling of the temperature field and ensure stable temperature changes. The observation window allows for real-time observation of the freeze-thawed raw materials to ensure the smooth progress of the freeze-thaw process. The raw materials are stirred by a stirring motor and stirring blades to ensure the uniformity of the freeze-thaw process. At the same time, with the assistance of the downward stirring device, the speed of material exchange between the upper and lower parts of the raw material in the chamber is increased, further improving the uniformity of freeze-thaw operation. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the entire utility model;

[0016] Figure 2 This is a three-dimensional structural diagram of the extractor body and the inlet closure assembly of this utility model;

[0017] Figure 3 This is a schematic diagram of the structure of the integral raw material freeze-thaw assembly of this utility model after being cut open and disassembled;

[0018] Figure 4 This is a three-dimensional structural diagram of the raw material freeze-thaw assembly of this utility model after being partially cut open and removed.

[0019] In the diagram: 1. Extractor body; 101. Feed inlet; 102. Limiting ring; 103. Limiting strip; 2. Inlet closing assembly; 201. Electric telescopic rod; 202. Closing plate; 3. Raw material freeze-thaw assembly; 301. Freeze-thaw shell; 302. Limiting block; 303. Discharge valve pipe; 304. Freeze-thaw chamber; 305. Heating platform; 306. Cooling ring plate; 307. Temperature sensor; 308. Addition port; 309. Temperature display panel; 310. Observation window; 311. Fixing block; 312. Stirring motor; 313. Stirring rod; 314. Arc-shaped stirring blade; 315. Scraper-type stirring blade; 316. Control box; 317. Downward stirring device. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0021] like Figures 1 to 4 As shown, a polypeptide extraction device includes an extractor body 1, an inlet 101 on the top surface of the extractor body 1, a limiting ring 102 on the top surface of the extractor body 1 that fits the outer side, an inlet closure component 2 covering the inlet 101 inside the limiting ring 102, and a detachable raw material freeze-thaw component 3 on the limiting ring 102.

[0022] like Figure 1 As shown, the main improvement of this invention lies in solving the problems of external contamination and temperature changes caused by separating freeze-thaw processing and peptide extraction, such as... Figures 1 to 4As shown, in this utility model, a polypeptide extraction device can be quickly installed on the extractor body 1 by aligning the limiting block 302 with the limiting strip 103 before use. During the placement of the raw material freeze-thaw assembly 3, when the discharge valve pipe 303 approaches the feed inlet 101, the electric telescopic rod 201 will extend outward, causing the closing plate 202 on the feed inlet 101 to unfold, ensuring that the discharge valve pipe 303 is placed smoothly. After the raw material freeze-thaw assembly 3 is placed, the raw material freeze-thaw assembly 3 can be quickly fixed on the extractor body 1 by passing a pin through the limiting ring 102 and the fixing block 311, ensuring structural stability and completing the installation.

[0023] In use, raw materials are first added to the freeze-thaw chamber 304 through the addition port 308. Then, the heating platform 305 and cooling ring plate 306 on the outside of the freeze-thaw chamber 304 are controlled. With the cooperation of the temperature sensor 307 and temperature display panel 309, the raw materials undergo a heating-freezing cycle to ensure that the temperature change is within a suitable range, thus achieving freeze-thaw treatment of the peptide raw materials. During the processing, when the extract is still liquid, the stirring motor 312 drives the stirring rod 313 to rotate, which in turn drives the arc-shaped stirring blade 314 and the scraper-type stirring blade 315 to slowly stir within the freeze-thaw chamber 304, achieving two-stage stirring of the extract. This ensures the uniformity of the freeze-thaw process and improves mixing efficiency. While the stirring motor 312 is working, the control box 316 operates the downward stirring device 317 to stir the raw materials in the freeze-thaw chamber 304 vertically, increasing the speed of vertical exchange of raw materials within the freeze-thaw chamber 304 and further improving the uniformity of the freeze-thaw process. After processing, the feed valve 303 in the feed inlet 101 will open, and the frozen and thawed raw material in the freeze-thaw chamber 304 will be sent into the extractor body 1 for other processes, ultimately achieving the extraction of peptides.

[0024] like Figure 2 As shown, the inlet closure assembly 2 includes an electric telescopic rod 201 disposed on both sides of the inner wall of the limiting ring 102. The end of the electric telescopic rod 201 is connected to a closing plate 202. The symmetrically arranged closing plates 202 extend and retract under the drive of the electric telescopic rod 201. When closed, they fit together to cover the feed inlet 101. The top surface of the extractor body 1 is provided with a limiting strip 103 that fits both sides of the closing plate 202.

[0025] Specifically, when the raw material freeze-thaw assembly 3 needs to be cleaned and maintained, as the raw material freeze-thaw assembly 3 is disassembled and removed, the discharge valve pipe 303 will be taken out from the feed port 101. Then, the symmetrically arranged closing plates 202 will extend and retract inward under the drive of the electric telescopic rod 201. When closed, they will fit together to cover the feed port 101, thereby sealing the feed port 101 and preventing contamination. The setting of the limiting strip 103 can limit the closing plate 202, prevent misalignment when the closing plate 202 extends and retracts, and improve the structural stability.

[0026] like Figure 1 , Figure 3 and Figure 4 As shown, the raw material freeze-thaw assembly 3 includes a freeze-thaw shell 301 fitted to the top surface of the limiting ring 102. A limiting block 302 fitted to the outside of the limiting strip 103 is located on the bottom surface of the freeze-thaw shell 301. A discharge valve pipe 303 is disposed downwards in the middle of the bottom surface of the freeze-thaw shell 301 and is placed inside the feed inlet 101. A freeze-thaw chamber 304 fixedly connected to the discharge valve pipe 303 is disposed inside the freeze-thaw shell 301. The lower end of the freeze-thaw chamber 304 is frustoconical and the upper end is cylindrical. A heating platform 305 is fitted to the lower outer side of the freeze-thaw chamber 304, and a cooling ring plate 306 is fitted to the upper outer side of the freeze-thaw chamber 304. Temperature sensors 307 are installed on the inner walls of both the upper and lower ends of the freeze-thaw chamber 304. Six sets of temperature sensors 307 are evenly arranged circumferentially on the inner wall of the freeze-thaw chamber 304. A filling port 308 leading to the freeze-thaw chamber 304 is provided on one side of the top surface of the freeze-thaw outer shell 301. A temperature display panel 309 connected to the temperature sensors 307 is provided next to the filling port 308.

[0027] Specifically, when it is necessary to freeze-thaw the peptide raw material, the raw material is first added to the freeze-thaw chamber 304 through the addition port 308. Then, the heating platform 305 and the cooling ring plate 306 set on the outside of the freeze-thaw chamber 304 are controlled. With the cooperation of the temperature sensor 307 and the temperature display panel 309, the raw material is heated-frozen in a cycle to realize the freeze-thaw treatment of the peptide raw material. After the treatment is completed, the discharge valve pipe 303 in the feed port 101 will be opened to send the frozen and thawed raw material in the freeze-thaw chamber 304 into the extractor body 1 for other processes, and finally realize the extraction of peptides.

[0028] like Figure 3 As shown, an observation window 310 is provided on the top surface of the freeze-thaw shell 301 on the symmetrical side of the addition port 308.

[0029] Specifically, by setting up an observation window 310, operators can observe the extraction process in real time and adjust the operating parameters in a timely manner.

[0030] like Figure 1 , Figure 3 and Figure 4As shown, the bottom surface of the freeze-thaw shell 301 is symmetrically provided with a fixing block 311 that fits the inner wall of the limiting ring 102. The fixing block 311 and the limiting ring 102 are fixedly connected by a pin.

[0031] Specifically, with the cooperation of the fixing block 311 and the limiting ring 102, the two can be fixed by using a pin, which can realize the quick fixing and disassembly of the extractor body 1 and the raw material freeze-thaw assembly 3, which is convenient for cleaning and maintenance and improves the efficiency of equipment use.

[0032] like Figure 3 As shown, a stirring motor 312 is provided in the middle of the top surface of the freeze-thaw shell 301. The drive shaft of the stirring motor 312 is connected to a stirring rod 313. The stirring rod 313 is located inside the freeze-thaw chamber 304. An arc-shaped stirring blade 314 is provided on the stirring rod 313 at the upper end of the freeze-thaw chamber 304, and a scraper-type stirring blade 315 that fits against the inner wall of the freeze-thaw chamber 304 is provided on the stirring rod 313 at the lower end of the freeze-thaw chamber 304.

[0033] Specifically, during the freeze-thaw process, when the extract is still in a liquid state, the stirring rod 313 drives the arc-shaped stirring blade 314 and the scraper-type stirring blade 315 to slowly stir the extract through the driving of the stirring motor 312. This achieves two-stage stirring of the extract, improves mixing efficiency, and ensures the uniformity of the raw material freeze-thaw process.

[0034] like Figure 3 As shown, control boxes 316 are provided on both sides of the top surface of the freeze-thaw shell 301. A pressure stirrer 317 is provided on the bottom surface of the control box 316 and is attached to the upper inner wall of the freeze-thaw chamber 304. The pressure stirrer 317 and the arc-shaped stirring blade 314 are kept in non-contact.

[0035] Specifically, the pressure mixer 317 is controlled by the control box 316. When the stirring motor 312 is working, the pressure mixer 317 will stir the raw materials in the freeze-thaw chamber 304 up and down, thereby increasing the speed of the exchange of raw materials in the freeze-thaw chamber 304 and further improving the uniformity of the freeze-thaw process. The pressure mixer 317 will not come into contact with the arc-shaped stirring blade 314, thus avoiding damage caused by collision.

[0036] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A polypeptide extraction device, comprising an extractor body (1), wherein a feed inlet (101) is provided on the top surface of the extractor body (1), characterized in that, The extractor body (1) has a limiting ring (102) on its top surface that fits the outer side. The limiting ring (102) has an inlet closing component (2) that covers the feed inlet (101) inside. The limiting ring (102) has a detachable raw material freeze-thaw component (3).

2. The polypeptide extraction device according to claim 1, characterized in that, The inlet closing assembly (2) includes an electric telescopic rod (201) disposed on both sides of the inner wall of the limiting ring (102). The end of the electric telescopic rod (201) is connected to a closing plate (202). The symmetrically arranged closing plates (202) extend and retract under the drive of the electric telescopic rod (201). When closed, they fit together to cover the feed inlet (101). The top surface of the extractor body (1) is provided with limiting strips (103) that fit both sides of the closing plate (202).

3. The polypeptide extraction device according to claim 2, characterized in that, The raw material freeze-thaw assembly (3) includes a freeze-thaw shell (301) fitted to the top surface of the limiting ring (102), a limiting block (302) fitted to the outside of the limiting strip (103) on the bottom surface of the freeze-thaw shell (301), a discharge valve pipe (303) disposed downward in the middle of the bottom surface of the freeze-thaw shell (301), the discharge valve pipe (303) being placed inside the feed inlet (101), and a freeze-thaw chamber (304) fixedly connected to the discharge valve pipe (303) inside the freeze-thaw shell (301). The freeze-thaw chamber (304) has a frustum shape at the lower end and a cylindrical shape at the upper end. A heating platform (305) is provided on the outer side of the lower end of the freeze-thaw chamber (304), and a cooling ring plate (306) is provided on the outer side of the upper end of the freeze-thaw chamber (304). Temperature sensors (307) are provided on the inner walls of both the upper and lower ends of the freeze-thaw chamber (304). Six sets of temperature sensors (307) are evenly arranged circumferentially on the inner wall of the freeze-thaw chamber (304). A filling port (308) leading to the freeze-thaw chamber (304) is provided on one side of the top surface of the freeze-thaw shell (301). A temperature display panel (309) connected to the temperature sensors (307) is provided next to the filling port (308).

4. The polypeptide extraction device according to claim 3, characterized in that, The top surface of the freeze-thaw shell (301) is provided with an observation window (310) on the symmetrical side of the addition port (308).

5. A polypeptide extraction device according to claim 3 or 4, characterized in that, The freeze-thaw shell (301) is symmetrically provided with a fixing block (311) that fits the inner wall of the limiting ring (102) and the fixing block (311) and the limiting ring (102) are fixedly connected by a pin.

6. A polypeptide extraction device according to claim 3 or 4, characterized in that, A stirring motor (312) is provided in the middle of the top surface of the freeze-thaw shell (301). The drive shaft of the stirring motor (312) is connected to a stirring rod (313). The stirring rod (313) is located inside the freeze-thaw chamber (304). An arc-shaped stirring blade (314) is provided on the stirring rod (313) at the upper end of the freeze-thaw chamber (304), and a scraper-type stirring blade (315) that fits against the inner wall of the freeze-thaw chamber (304) is provided on the stirring rod (313) at the lower end of the freeze-thaw chamber (304).

7. The polypeptide extraction device according to claim 5, characterized in that, A stirring motor (312) is provided in the middle of the top surface of the freeze-thaw shell (301). The drive shaft of the stirring motor (312) is connected to a stirring rod (313). The stirring rod (313) is located inside the freeze-thaw chamber (304). An arc-shaped stirring blade (314) is provided on the stirring rod (313) at the upper end of the freeze-thaw chamber (304), and a scraper-type stirring blade (315) that fits against the inner wall of the freeze-thaw chamber (304) is provided on the stirring rod (313) at the lower end of the freeze-thaw chamber (304).

8. The polypeptide extraction device according to claim 6, characterized in that, Control boxes (316) are provided on both sides of the top surface of the freeze-thaw shell (301). A pressure stirrer (317) is provided on the bottom surface of the control box (316) and is attached to the inner wall of the upper end of the freeze-thaw chamber (304). The pressure stirrer (317) and the arc-shaped stirring blade (314) are kept in non-contact.

9. The polypeptide extraction device according to claim 7, characterized in that, Control boxes (316) are provided on both sides of the top surface of the freeze-thaw shell (301). A pressure stirrer (317) is provided on the bottom surface of the control box (316) and is attached to the inner wall of the upper end of the freeze-thaw chamber (304). The pressure stirrer (317) and the arc-shaped stirring blade (314) are kept in non-contact.

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