Pathogen targeted sequencing sample preparation system

Abstract

The utility model relates to the field of medical instruments and discloses a pathogen targeted sequencing sample preparation system which comprises a frame assembly, a mechanical arm assembly, a gas circulation assembly and a shell assembly. The working platform is provided with a sample adding needle box mounting module, a sample plate mounting module, a normal-temperature reagent plate mounting module, a cold storage reagent plate mounting module, a fluorescence detection module, a magnetic attraction module, an oscillation incubation module, a PCR module, a waste needle and waste liquid receiving structure and a cover plate receiving module, and the mechanical arm assembly comprises a pipetting mechanical arm, a plate grabbing and moving mechanical arm and a first driving assembly. The gas circulation assembly comprises a gas inlet assembly and a gas exhaust assembly which are mounted on the frame assembly and / or the shell assembly, the gas outlet end of the gas inlet assembly corresponds to the PCR module in position, and the gas inlet end of the gas exhaust assembly corresponds to the PCR module in position. The pathogen targeted sequencing sample preparation system disclosed by the utility model can prevent hot air generated by the operation of the PCR module from running inside the system.

Description

Technical Field

[0001] This utility model relates to the field of medical devices, specifically to a pathogen-targeted sequencing sample preparation system. Background Technology

[0002] Pathogen-targeted sequencing (tNGS) is a next-generation sequencing technology that combines ultra-multiplex PCR amplification (or targeted capture) with high-throughput sequencing. It can directly enrich dozens to hundreds of known pathogenic microorganisms and their virulence and / or drug resistance genes in clinical samples without relying on traditional microbial culture. High-throughput sequencing is then performed, and the results are compared with a database to determine the types of pathogenic microorganisms present in the sample. Targeted sequencing can rapidly and objectively detect a large number of pathogenic microorganisms in clinical samples, providing a basis for clinical diagnosis. It is particularly advantageous for detecting low concentrations of pathogenic microorganisms, especially their virulence and / or drug resistance genes. However, current applications typically use general-purpose gene sequencing sample preparation systems to prepare tNGS samples. Existing gene sequencing sample preparation systems usually only have one air intake component at the top. During the preparation of tNGS samples, the large amount of hot air generated by the PCR module can circulate within the system, posing a risk of cross-contamination of the experimental samples.

[0003] In view of this, there is a need in the art for a pathogen-targeted sequencing sample preparation system that can prevent the hot air generated by the operation of the PCR module from running wildly inside the system. Summary of the Invention

[0004] The purpose of this invention is to provide a pathogen-targeted sequencing sample preparation system that can prevent the hot air generated during PCR module operation from escaping within the system.

[0005] To achieve the above objectives, this utility model provides a pathogen-targeted sequencing sample preparation system, comprising: a frame assembly, the frame assembly having an internal working platform, the working platform having a sample dispensing needle box mounting module, a sample plate mounting module, a room temperature reagent plate mounting module, a refrigerated reagent plate mounting module, a fluorescence detection module, a magnetic suction module, a shaking incubation module, a PCR module, a waste needle and waste liquid receiving structure, and a cover plate receiving module for receiving and storing PCR cover plates; and a robotic arm assembly, the robotic arm assembly being disposed inside the frame assembly and located above the working platform. The robotic arm assembly includes a pipetting robotic arm for transferring liquid samples by attaching a sampling needle, a plate gripping and transferring robotic arm for holding and transferring a deep well plate or a PCR cover plate, and a first drive assembly for driving the pipetting robotic arm and the plate gripping and transferring robotic arm to move along a first direction; a housing assembly mounted on the frame assembly; and a gas circulation assembly including an inlet assembly and an exhaust assembly mounted on the frame assembly and / or the housing assembly, wherein the outlet end of the inlet assembly corresponds to the position of the PCR module, and the inlet end of the exhaust assembly corresponds to the position of the PCR module.

[0006] Specifically, the frame assembly has a platform back plate perpendicular to the working platform inside, the air intake assembly is installed on the platform back plate, the platform back plate has a grille air intake opening corresponding to the air intake assembly, and the rear of the outer shell assembly has a first clearance opening corresponding to the air intake assembly.

[0007] Specifically, the air intake assembly includes: a first housing, the interior of which is provided with an air intake channel, and the first housing is mounted on the platform back plate; a first filter element, which is mounted inside the first housing and located in the air intake channel; and a first fan assembly, which is mounted inside the first housing and located in the air intake channel.

[0008] Specifically, the exhaust assembly is mounted on the top of the housing assembly, and the top of the frame assembly is provided with a second clearance opening corresponding to the exhaust assembly.

[0009] Specifically, the exhaust assembly includes: a second housing with an exhaust channel inside, the second housing being mounted on top of the outer casing assembly; a second filter element installed inside the second housing and located in the exhaust channel; and a second fan assembly installed inside the second housing and located in the exhaust channel.

[0010] Specifically, the pipetting robot arm includes a first robot arm frame assembly movably mounted on the frame assembly along the first direction, a pipetting pump module movably mounted on the first robot arm frame assembly along the second direction, and a pipetting pump module drive assembly for driving the pipetting pump module to move along the second direction. The pipetting pump module is used to transfer liquid samples by installing a sampling needle and can extend and retract along a third direction. The first direction, the second direction, and the third direction are perpendicular to each other.

[0011] Specifically, the gripping and moving robotic arm includes a second robotic arm frame assembly movably mounted on the frame assembly along the first direction, a clamping module movably mounted on the second robotic arm frame assembly along the second direction, and a clamping module driving assembly for driving the clamping module to move along the second direction. The clamping module is used to clamp a deep well plate or a PCR cover plate and can extend and retract along the third direction.

[0012] Specifically, the first robotic arm frame assembly and the second robotic arm frame assembly are fastened together.

[0013] The pathogen-targeted sequencing sample preparation system of this invention incorporates a gas circulation component for the pathogen-targeted sequencing sample preparation process. This component guides the large volume of hot air generated by the PCR module within the system to flow in a predetermined direction, preventing the hot air from wandering within the system and thus avoiding the risk of cross-contamination of the experimental samples.

[0014] Other features and advantages of this invention will be described in detail in the following detailed description section. Attached Figure Description

[0015] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the following detailed description to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0016] Figure 1 This is a perspective view of a specific embodiment of the pathogen-targeted sequencing sample preparation system according to this utility model;

[0017] Figure 2 yes Figure 1 A three-dimensional view of the pathogen-targeted sequencing sample preparation system from another perspective;

[0018] Figure 3 yes Figure 1 A 3D view of the pathogen-targeted sequencing sample preparation system after the outer shell components have been hidden.

[0019] Figure 4 yes Figure 3 An exploded view of the pathogen-targeted sequencing sample preparation system in the image;

[0020] Figure 5 yes Figure 3 An exploded view of the pathogen-targeted sequencing sample preparation system from another perspective;

[0021] Figure 6 yes Figure 3 An exploded view of the robotic arm component in the pathogen-targeted sequencing sample preparation system.

[0022] Explanation of reference numerals in the attached figures

[0023] 1. Frame assembly 2. Robotic arm assembly

[0024] 3. Housing assembly; 4. Gas circulation assembly

[0025] 11. Work platform 12. Platform backplane

[0026] 13. Second clearance opening

[0027] 21. Liquid pipetting robot arm; 22. Plate-grabbing robot arm

[0028] 23. First drive component

[0029] 31. First clearance opening

[0030] 41. Intake assembly 42. Exhaust assembly

[0031] 111. Sample dispensing needle box mounting module; 112. Sample plate mounting module

[0032] 113. Room temperature reagent plate mounting module; 114. Refrigerated reagent plate mounting module

[0033] 115. Fluorescence detection module; 116. Magnetic module

[0034] 117. Shaking Incubation Module; 118. PCR Module

[0035] 119. Waste needle and waste liquid receiving structure; 120. Cover plate receiving module

[0036] 121. Grille air intake opening

[0037] 211. First robotic arm frame assembly; 212. Pipette pump module

[0038] 213. Pipette pump module drive assembly

[0039] 221. Second robotic arm frame assembly; 222. Clamping module

[0040] 223. Clamping Module Driver Component

[0041] 411. First shell

[0042] 421. Second shell

[0043] A. First direction B. Second direction

[0044] C. Third-party orientation. Detailed Implementation

[0045] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are only for illustration and explanation of the embodiments of this application and are not intended to limit the embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application.

[0046] It should be noted that if the embodiments of this application involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, features defined with "first" or "second" may explicitly or implicitly include at least one of those features. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. If the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed in this application.

[0047] This utility model provides a pathogen-targeted sequencing sample preparation system, specifically, as follows: Figures 1 to 6As shown, the pathogen-targeted sequencing sample preparation system includes: a frame assembly 1, which has a working platform 11 inside. The working platform 11 includes a sample needle holder mounting module 111, a sample plate mounting module 112, a room temperature reagent plate mounting module 113, a refrigerated reagent plate mounting module 114, a fluorescence detection module 115, a magnetic suction module 116, a shaking incubation module 117, a PCR module 118, a waste needle and waste liquid receiving structure 119, and a cover plate receiving module 120 for receiving and storing PCR cover plates; and a robotic arm assembly 2, which is located inside the frame assembly 1 and above the working platform 11. The robotic arm assembly 2 includes components for... A pipetting robotic arm 21 for transferring liquid samples by attaching a sampling needle; a plate-gripping robotic arm 22 for gripping and transferring a deep-well plate or PCR cover plate; and a first drive assembly 23 for driving the pipetting robotic arm 21 and the plate-gripping robotic arm 22 to move along a first direction A; a housing assembly 3, which is mounted on the frame assembly 1; and a gas circulation assembly 4, which includes an air inlet assembly 41 and an air outlet assembly 42 mounted on the frame assembly 1 and / or the housing assembly 3, wherein the air outlet of the air inlet assembly 41 corresponds to the position of the PCR module 118, and the air inlet of the air outlet assembly 42 corresponds to the position of the PCR module 118.

[0048] The pathogen-targeted sequencing sample preparation system of this invention incorporates a gas circulation component for the pathogen-targeted sequencing sample preparation process. This allows the gas circulation component 4 to guide the large amount of hot air generated by the PCR module 118 in the pathogen-targeted sequencing sample preparation system to flow in a predetermined direction, preventing the hot air from wandering around inside the system and thus avoiding the risk of cross-contamination of the experimental samples.

[0049] It should be noted that the specific structural forms or types of the frame component 1, working platform 11, sample needle box installation module 111, sample plate installation module 112, room temperature reagent plate installation module 113, refrigerated reagent plate installation module 114, fluorescence detection module 115, magnetic suction module 116, shaking incubation module 117, PCR module 118, waste needle and waste liquid receiving structure 119, cover plate receiving module 120, pipetting robotic arm 21, plate gripping and transferring robotic arm 22, first drive component 23, outer shell component 3, air inlet component 41 and air outlet component 42 can all be set as needed, and these all fall within the technical concept scope of this utility model. It should also be noted that the specific structural forms of the above-mentioned sample loading needle box installation module 111, sample plate installation module 112, room temperature reagent plate installation module 113, refrigerated reagent plate installation module 114, fluorescence detection module 115, magnetic suction module 116, shaking incubation module 117, PCR module 118, waste needle and waste liquid receiving structure 119, and cover plate receiving module 120 can adopt existing forms in this or similar fields, and therefore will not be described in detail.

[0050] In the pathogen-targeted sequencing sample preparation system of this invention, specifically, as follows: Figure 2 and Figure 4 As shown, the frame assembly 1 has a platform back plate 12 perpendicular to the working platform 11 inside. The air intake assembly 41 is installed on the platform back plate 12. The platform back plate 12 has a grille air intake opening 121 corresponding to the air intake assembly 41. The rear of the outer shell assembly 3 has a first clearance opening 31 corresponding to the air intake assembly 41. This allows the air intake assembly 41 to be positioned at the rear of the pathogen-targeted sequencing sample preparation system of this invention, facilitating air intake while also possessing the advantages of a reasonable and simple structural design. More specifically, as... Figure 5 As shown, the air intake assembly 41 includes: a first housing 411, the first housing 411 having an air intake channel inside, and the first housing 411 being mounted on the platform back plate 12; a first filter element (not shown in the figure), the first filter element being mounted inside the first housing 411 and located in the air intake channel; and a first fan assembly (not shown in the figure), the first fan assembly being mounted inside the first housing 411 and located in the air intake channel.

[0051] In the aforementioned pathogen-targeted sequencing sample preparation system, more specifically, such as Figure 1 and Figure 2As shown, the exhaust assembly 42 is installed on the top of the outer shell assembly 3. The top of the frame assembly 1 is provided with a second clearance opening 13 corresponding to the exhaust assembly 42. This allows the exhaust assembly 42 to be positioned on top of the pathogen-targeted sequencing sample preparation system of this invention, facilitating air venting and avoiding interference from external exhaust flow to experimental personnel, while also possessing the advantages of a reasonable and simple structural design. More specifically, the exhaust assembly 42 includes: a second housing 421, with an exhaust channel inside the second housing 421, and the second housing 421 is installed on the top of the outer shell assembly 3; a second filter element, installed inside the second housing 421 and located within the exhaust channel; and a second fan assembly, installed inside the second housing 421 and located within the exhaust channel.

[0052] In the aforementioned pathogen-targeted sequencing sample preparation system, more specifically, such as Figure 6 As shown, the pipetting robotic arm 21 includes a first robotic arm frame assembly 211 movably mounted on the frame assembly 1 along the first direction A, a pipetting pump module 212 movably mounted on the first robotic arm frame assembly 211 along the second direction B, and a pipetting pump module drive assembly 213 for driving the pipetting pump module 212 to move along the second direction B. The pipetting pump module 212 is used to transfer liquid samples by attaching a sampling needle and can extend and retract along a third direction C. The first direction A, the second direction B, and the third direction C are perpendicular to each other. The plate-gripping robotic arm 22 includes a second robotic arm frame assembly 221 movably mounted on the frame assembly 1 along the first direction A, a clamping module 222 movably mounted on the second robotic arm frame assembly 221 along the second direction B, and a clamping module drive assembly 223 for driving the clamping module 222 to move along the second direction B. The clamping module 222 is used to clamp a deep well plate or a PCR cover plate and can extend and retract along the third direction C.

[0053] It should be noted that the specific structural forms of the first robotic arm frame assembly 211, the pipetting pump module 212, the pipetting pump module drive assembly 213, the second robotic arm frame assembly 221, the clamping module 222, and the clamping module drive assembly 223 can adopt existing forms in this or similar fields, and therefore will not be described in detail.

[0054] In the above-mentioned pathogen-targeted sequencing sample preparation system, preferably, the first robotic arm frame assembly 211 and the second robotic arm frame assembly 221 are fastened to each other, so that the first driving assembly 23 can be used to drive the pipetting robotic arm 21 and the gripper-plate transfer robotic arm 22 to move along the first direction A, thereby achieving the purpose of reducing costs (avoiding the need to set up two sets of driving assemblies to drive the pipetting robotic arm 21 and the gripper-plate transfer robotic arm 22 to move along the first direction A).

[0055] The preferred embodiments of this application have been described in detail above with reference to the accompanying drawings. However, this application is not limited to the specific details of the above embodiments. Within the scope of the technical concept of this application, various simple modifications can be made to the technical solution of this application, and these simple modifications all fall within the protection scope of this application.

[0056] It should also be noted that the various specific technical features described in the above embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, this application will not describe the various possible combinations separately.

[0057] Furthermore, various different implementations of this application can be combined in any way, as long as they do not violate the spirit of this application, they should also be regarded as the content disclosed in this application.

Claims

1. A pathogen-targeted sequencing sample preparation system, characterized in that, The pathogen-targeted sequencing sample preparation system includes: The frame assembly (1) has a working platform (11) inside. The working platform (11) is equipped with a sample needle box installation module (111), a sample plate installation module (112), a room temperature reagent plate installation module (113), a refrigerated reagent plate installation module (114), a fluorescence detection module (115), a magnetic suction module (116), a shaking incubation module (117), a PCR module (118), a waste needle and waste liquid receiving structure (119), and a cover plate receiving module (120) for receiving and storing PCR cover plates. The robotic arm assembly (2) is disposed inside the frame assembly (1) and above the work platform (11). The robotic arm assembly (2) includes a pipetting robotic arm (21) for transferring liquid samples by installing a sampling needle, a plate gripping and transferring robotic arm (22) for gripping and transferring a deep well plate or PCR cover plate, and a first drive assembly (23) for driving the pipetting robotic arm (21) and the plate gripping and transferring robotic arm (22) to move along a first direction (A). Housing assembly (3), said housing assembly (3) being mounted on said frame assembly (1); and The gas circulation assembly (4) includes an air intake assembly (41) and an exhaust assembly (42) installed on the frame assembly (1) and / or the outer shell assembly (3), wherein the exhaust end of the air intake assembly (41) corresponds to the position of the PCR module (118), and the air intake end of the exhaust assembly (42) corresponds to the position of the PCR module (118).

2. The pathogen-targeted sequencing sample preparation system according to claim 1, characterized in that, The frame assembly (1) has a platform back plate (12) perpendicular to the working platform (11) inside. The air intake assembly (41) is installed on the platform back plate (12). The platform back plate (12) has a grille air intake opening (121) corresponding to the air intake assembly (41). The rear of the outer shell assembly (3) has a first clearance opening (31) corresponding to the air intake assembly (41).

3. The pathogen-targeted sequencing sample preparation system according to claim 2, characterized in that, The intake assembly (41) includes: The first housing (411) has an air intake channel inside and is mounted on the platform back plate (12). A first filter element is installed inside the first housing (411) and located in the air intake passage; and The first fan assembly is installed inside the first housing (411) and located in the air intake channel.

4. The pathogen-targeted sequencing sample preparation system according to claim 1 or 2, characterized in that, The exhaust assembly (42) is mounted on the top of the housing assembly (3), and the top of the frame assembly (1) is provided with a second clearance opening (13) corresponding to the exhaust assembly (42).

5. The pathogen-targeted sequencing sample preparation system according to claim 4, characterized in that, The exhaust assembly (42) includes: The second housing (421) has an exhaust channel inside and is mounted on the top of the outer casing assembly (3); A second filter element is installed inside the second housing (421) and located in the exhaust passage; and The second fan assembly is installed inside the second housing (421) and located in the exhaust passage.

6. The pathogen-targeted sequencing sample preparation system according to claim 1 or 2, characterized in that, The pipetting robot (21) includes a first robot frame assembly (211) movably mounted on the frame assembly (1) along the first direction (A), a pipetting pump module (212) movably mounted on the first robot frame assembly (211) along the second direction (B), and a pipetting pump module drive assembly (213) for driving the pipetting pump module (212) to move along the second direction (B). The pipetting pump module (212) is used to transfer liquid samples by installing a sampling needle and is capable of extending and retracting along a third direction (C). The first direction (A), the second direction (B), and the third direction (C) are perpendicular to each other.

7. The pathogen-targeted sequencing sample preparation system according to claim 6, characterized in that, The gripper and transfer robot arm (22) includes a second robot arm frame assembly (221) movably mounted on the frame assembly (1) along the first direction (A), a gripping module (222) movably mounted on the second robot arm frame assembly (221) along the second direction (B), and a gripping module drive assembly (223) for driving the gripping module (222) to move along the second direction (B). The gripping module (222) is used to grip a deep well plate or a PCR cover plate and can extend and retract along the third direction (C).

8. The pathogen-targeted sequencing sample preparation system according to claim 7, characterized in that, The first robotic arm frame assembly (211) and the second robotic arm frame assembly (221) are fastened together.

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