Auxiliary positioning sample adding plate

Abstract

The utility model relates to the field of biomedical experiment auxiliary tools, and discloses an auxiliary positioning sample adding plate which comprises a sample adding main plate, one side of the sample adding main plate is provided with tooth protrusions used for being embedded into corresponding tooth grooves of a glue making comb, and the tooth protrusions are distributed on the edge of a sample adding area on the upper portion of the sample adding main plate at equal intervals along a straight line. And the comb teeth of the glue making comb are correspondingly embedded into a gap between two adjacent tooth protrusions. Tooth protrusions of the sample adding main plate are clamped with tooth grooves of the glue making comb, positioning can be achieved in the glue making process, and it can be ensured that after the glue making comb is taken out, visible reference is provided for the sample adding hole; the identification frame is arranged on the sample adding main board, a clear sample adding area boundary is formed, an experimenter can be guided to accurately position the sample loading hole under the condition that the sample adding plate is not removed, and under the positioning of the odontoid, it can be ensured that the hole position of the sample loading hole accurately corresponds to the identification frame; the problem that the sample loading hole is difficult to position due to manual experience-dependent positioning is solved, and the sample loading accuracy and the operation efficiency are improved.

Description

Technical Field

[0001] This utility model relates to the field of biomedical experimental auxiliary tools, specifically to an auxiliary positioning and template. Background Technology

[0002] In Western blot experiments, the loading of protein electrophoresis gel is one of the key steps, and the accuracy of the operation directly affects the subsequent electrophoretic separation effect and result analysis. In the protein electrophoresis stage of the experiment, glass plates are the key equipment used in the gel preparation process. They are usually used in pairs, with two plates fixed together by gel clamps or fixtures to form a "sandwich" structure that is sealed at the bottom and sides, with a gel interlayer in the middle and an opening at the top for pouring gel solution into the interlayer.

[0003] Current status of Western blot experiments:

[0004] (1) Protein electrophoresis sample loading is often done manually. The experimenter aligns the sample well with the naked eye. The accuracy of sample loading depends on personal experience. However, manual positioning is highly subjective and there is a sample loading deviation.

[0005] (2) The glass plate is not marked, which can easily lead to inaccurate sample addition and sample omission; or the sticker / hand-drawn marking is used, but it has the defects of being easy to fall off, not being washable and having poor repeatability;

[0006] (3) The operation of multi-well samples is not numbered, and the disordered loading of samples can easily lead to sample confusion and recording difficulties.

[0007] One approach is to use a glue-forming comb to create holes during glue preparation. However, after combing, there is no visible reference, and the glue and glass are prone to misalignment, which can easily lead to misalignment of the sample holes. Therefore, there is an urgent need to propose a sample plate with auxiliary positioning and numbering functions to solve the above problems. Utility Model Content

[0008] The purpose of this utility model is to overcome the shortcomings of the existing technology and provide an auxiliary positioning template. The template uses the toothed protrusions on its side to engage with the toothed grooves of the glue-making comb to achieve the positioning of the glue-making comb during the glue-making process and ensure that the sample hole position is accurately aligned after the glue-making comb is removed, without any misalignment.

[0009] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0010] An auxiliary positioning sample loading plate includes a sample loading main plate. One side of the sample loading main plate is provided with tooth protrusions for embedding into the corresponding tooth grooves of a glue-making comb. The tooth protrusions are distributed at equal intervals along a straight line at the edge of the sample loading area on the upper part of the sample loading main plate, and the comb teeth of the glue-making comb are correspondingly embedded in the gap between two adjacent tooth protrusions.

[0011] Optionally, the toothed protrusion and the sample loading main board adopt an integral molding structure.

[0012] Optionally, the toothed protrusion can be detachably installed on the inside of the sample application mainboard.

[0013] Optionally, the sample loading motherboard is made of borosilicate glass or tempered glass.

[0014] Optionally, the sample loading main board is provided with an identification frame for marking the outline and position of the sample loading hole, and the identification frame has a U-shaped structure.

[0015] Optionally, a number is provided below the identification frame, and the number corresponds one-to-one with the position of the sample loading hole.

[0016] Optionally, the identification frame and the number are both located on the inner side of the sample loading motherboard. The number and identification frame can be formed by laser etching or screen printing, and have good corrosion resistance, cleaning resistance and clarity, making them suitable for high-frequency reusable experimental environments.

[0017] Optionally, the length, width, and thickness of the sample loading board are 100mm, 80mm, and 2-3mm, respectively, and the height and width of the toothed protrusions are 0.3mm and 0.5mm, respectively.

[0018] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0019] In this invention, the toothed protrusions of the sample loading main board engage with the toothed grooves of the glue-making comb, enabling positioning during the glue-making process. Furthermore, it provides a visible reference for the sample loading hole after the glue-making comb is removed, while preventing misalignment between the sample loading hole on the colloid and the sample loading main board.

[0020] (2) In this utility model, an identification frame is set on the sample loading board to form a clear sample loading area boundary. This can guide the experimenter to accurately position the sample loading hole without removing the sample loading board. Furthermore, with the positioning of the toothed protrusion, it can ensure that the position of the sample loading hole corresponds precisely with the identification frame. This solves the problem of difficulty in positioning the sample loading hole caused by manual positioning based on experience, and improves the accuracy of sample loading and operational efficiency.

[0021] (3) The number in this utility model corresponds one-to-one with the position of the sample well, and the number is located below the identification frame. By numbering each sample well, the problem of sample confusion caused by lack of sequential numbering can be solved, and the accuracy of multi-well sample operation and the convenience of data recording can be improved.

[0022] (4) In this utility model, the sample loading main board and the toothed protrusion adopt an integral molding structure, which can ensure the overall structural strength of the sample loading board. The sample loading main board and the toothed protrusion adopt a detachable split structure, which can achieve the same positioning effect as the integral structure. Moreover, the split toothed protrusion is easier to replace to adapt to different sample loading hole sizes and quantities, thereby improving the applicability of the device. Attached Figure Description

[0023] Figure 1 This is an assembly diagram of the auxiliary positioning template, the template sub-plate, and the glue-making comb in an embodiment of this utility model;

[0024] Figure 2 This is an assembly diagram of the auxiliary positioning template and the glue-making comb in an embodiment of this utility model;

[0025] Figure 3 This is a schematic diagram of the engagement between the toothed protrusion and the toothed groove in an embodiment of this utility model;

[0026] Figure 4 This is a side view of the sample-adding motherboard in an embodiment of this utility model.

[0027] Figure 5 This is a schematic diagram of the inner and outer sides of the sample loading motherboard in an embodiment of this utility model;

[0028] Among them, 1. Sample loading main plate; 101. Tooth protrusion; 102. Identification frame; 2. Sample loading auxiliary plate; 3. Glue comb; 301. Comb teeth; 302. Tooth groove. Detailed Implementation

[0029] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. These drawings are simplified schematic diagrams, which are only used to illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0030] The gel preparation process in electrophoresis experiments requires a sample loading plate, a sample loading sub-plate 2, a gel casting comb 3, and other commonly used equipment. The process is as follows:

[0031] Sample assembly: Before making the glue, clean the sample plate and sample sub-plate 2, and after drying, assemble them in parallel onto the corresponding glue making frame to form a "sandwich" structure with a sealed bottom and sides, a gel interlayer in the middle, and an opening at the top for injecting glue into the interlayer.

[0032] Adding the lower layer adhesive: First, inject the prepared lower layer mixture into the interlayer between the two plates, then add the upper layer mixture to form the lower layer adhesive. Then, slightly lift the adhesive preparation frame and shake it until the lower layer adhesive surface is horizontal.

[0033] Adding the upper layer adhesive: Add the prepared upper layer adhesive mixture to the interlayer between the two plates, above the lower layer adhesive solution, until the interlayer space is full, and shake the adhesive preparation frame until the liquid level of the upper layer adhesive is horizontal;

[0034] After the gel solution is added, insert the gel-forming comb 3 vertically from top to bottom between the two plates, so that the comb teeth 301 of the gel-forming comb 3 are immersed in the gel solution between the layers. After the gel solution solidifies to form a colloid, pull out the gel-forming comb 3. The original position of the comb teeth 301 is hollow, thus forming a sample loading hole. Finally, put the two plates and the colloid into the electrophoresis apparatus. After adding the sample, the electrophoresis experiment can be carried out. Example 1

[0035] like Figures 1-4 As shown, an auxiliary positioning sample loading plate includes a sample loading main plate 1. The inner side of the sample loading main plate 1 (the side closer to the colloid) is provided with a plurality of toothed protrusions 101. The plurality of toothed protrusions 101 are distributed at equal intervals along a straight line on the upper part of the sample loading main plate 1 and are located at the edge of the sample loading area.

[0036] The rubber comb 3 includes multiple parallel comb teeth 301, and the multiple comb teeth 301 are distributed at equal intervals along a horizontal straight line, so that a tooth groove 302 is formed between two adjacent comb teeth 301.

[0037] The auxiliary positioning sample plate is designed with tiny toothed protrusions 101 on the contact surface between the sample plate 1 and the gel, which engage with the toothed grooves 302 of the gel comb 3. This allows for positioning during the gel preparation process, ensuring that the markings on the sample plate accurately correspond to the gel holes after the comb is removed, without any misalignment.

[0038] Specifically, after the sample plate and sample auxiliary plate 2 proposed in this utility model are installed together on the glue-making frame, the sample main plate 1 and sample auxiliary plate 2 are parallel to each other, and the two ends of the toothed protrusion 101 are in contact with the inner side surfaces of the sample main plate 1 and sample auxiliary plate 2 respectively. Under the action of the glue-making frame, a sealed interlayer space is formed between the sample main plate 1 and sample auxiliary plate 2 with the bottom and sides sealed. After injecting the corresponding glue into the interlayer space, the glue-making comb 3 is vertically inserted into the upper part of the interlayer space from top to bottom.

[0039] After the gel-forming comb 3 is inserted into the interlayer space, the comb teeth 301 extend into the gel solution through the gap between two adjacent tooth protrusions 101, causing the tooth protrusions 101 and the tooth grooves 302 to engage with each other, thus providing auxiliary positioning for the insertion of the gel-forming comb 3. After the gel solution solidifies into a colloid, the gel-forming comb 3 is removed. At this time, the position where the comb teeth 301 were originally is hollow, thus forming a sample loading hole at the top of the colloid for adding samples.

[0040] The toothed protrusion 101 of the sample loading main board 1 engages with the toothed groove 302 of the glue-making comb 3, which can achieve positioning of the glue-making comb 3 during the glue-making process. Moreover, it provides a visible reference for the sample loading hole after the glue-making comb 3 is removed, and avoids misalignment between the sample loading hole on the colloid and the sample loading main board 1, which greatly improves the stability and convenience of glue-making.

[0041] The sample addition plate 2 can be a regular glass sample addition plate or the auxiliary positioning sample addition plate proposed in this utility model.

[0042] like Figure 5 As shown, an identification frame 102 is provided between two adjacent toothed protrusions 101. The identification frame 102 has a U-shaped structure and is used to mark the outline and position of the sample hole on the sample plate. A number is provided below the identification frame 102, and the number corresponds one-to-one with the position of the sample hole.

[0043] in, Figure 5 The left image shows a schematic diagram of the outer side of the sample loading plate, and the right image shows a schematic diagram of the inner side of the sample loading plate. The numbering uses uppercase English letters sequentially from left to right to mark the corresponding sample loading wells, for example, recorded as well A, well B, well C, etc. The letter numbers corresponding to the positions of comb teeth 301 are pre-engraved on the sample loading plate to permanently mark the sequential numbering of the sample loading wells, which facilitates experimental recording and sample management, and supports reuse and cleaning.

[0044] To ensure that the identification frame 102 and the number can be used repeatedly over a long period of time, both the identification frame 102 and the number are located on the inner side of the sample loading plate 1, that is, on the side close to the toothed protrusion 101. Since the sample loading plate is usually made of a transparent material, such as glass, the position of the identification frame 102 and the number can be observed from the outside of the sample loading plate, thereby determining the position and order of the sample loading holes.

[0045] An etched frame line corresponding to the edge of the gel comb 3 is set on the sample loading plate to form an identification frame 102 corresponding to the sample loading hole, thus forming a clear sample loading area boundary corresponding to the sample loading hole. This can guide the experimenter to accurately position the sample loading hole without removing the sample loading plate. Furthermore, with the positioning of the toothed protrusion 101, it can ensure that the hole position of the sample loading hole corresponds precisely to the identification frame 102, solving the problem of difficulty in positioning the sample loading hole caused by manual positioning based on experience, and improving the accuracy of sample loading and operational efficiency.

[0046] The number corresponds one-to-one with the position of the sample loading well, and the number is located below the identification box 102. By numbering each sample loading well, the problem of sample confusion caused by lack of sequential numbering can be solved, and the accuracy of multi-well sample operation and the convenience of data recording can be improved.

[0047] Furthermore, the structure of the template in this utility model includes, but is not limited to, the following design:

[0048] Geometric parameters: The material can be high-strength borosilicate glass or tempered glass (thickness 2-3mm), and the size is the standard protein electrophoresis plate size (approximately 80mm×100mm).

[0049] Etching process: The frame lines and numbering of the identification frame 102 are laser etched (line width approximately 0.2mm). The markings are located on the inside of the glass plate (near the gel surface), making them corrosion resistant and colorfast.

[0050] Positioning structure: The height of the tooth protrusion is 0.3 mm and the width is 0.5 mm, which matches the size of the comb teeth 301. They are distributed at the edge of the sample application area, in contact with the colloid but without interfering with protein migration.

[0051] Compared to existing technologies, the problems include: manual positioning is highly subjective and prone to sample loading deviation; disordered sample loading can easily lead to sample confusion and recording difficulties; and after the comb is removed, the glass has no reference and the sample loading hole cannot be aligned.

[0052] The advantages of this solution are: the identification frame 102 and the glue comb 3 are designed in a synchronized manner, making sample addition more intuitive; permanent numbering marks support high-frequency cleaning and repeated use; and the structural alignment design eliminates the source of error between the colloid and the glass plate (misalignment < ±0.1mm).

[0053] In addition to directly etching the corresponding structure on the glass plate, the following alternative technologies can be used: using a screen-printed corrosion-resistant coating instead of etched lines, which has a certain degree of durability; the tooth protrusion 101 can be adhered to the sample loading motherboard 1 by a transparent silicone strip to achieve the same positioning effect and is easy to replace.

[0054] The technical advantages of this invention in terms of positioning accuracy, operational efficiency, marker stability, experimental repeatability, and pollution control are shown in the table below:

[0055]

[0056] The results of comparing traditional technical solutions are shown in the table below:

[0057]

[0058] In addition, there is a practice on the market of dyeing / serializing the top layer of adhesive. Although this can play a certain role in assisting positioning, this method has high requirements for the pre-made adhesive. There are few products available at present, and its universality is not strong. If the adhesive is made at home, it can only be dyed by the experimenter, which increases the workload of the experimenter.

[0059] The existing pre-made adhesives with markings are limited in number and cannot cover commonly used adhesive concentrations and specifications. Furthermore, in actual use, there may be reactions between the dye and the adhesive matrix (e.g., stacked adhesives become brittle after dyeing), leading to experimental failures. If a customized solution is adopted, it will increase the laboratory's procurement costs and inventory pressure.

[0060] When staining yourself, the dye concentration needs to be repeatedly tested through preliminary experiments, which increases the preparation time. If the dye is not selected properly (such as affecting protein migration or transfer), it may lead to deviations in experimental results (such as band tailing or decreased transfer efficiency).

[0061] Therefore, the existing practices mentioned above cannot balance the relationship between "accuracy of positioning", "ease of operation" and "cost controllability". Self-made adhesive labeling requires additional steps and also relies on experience. Pre-made adhesive solutions are limited by product types and costs and have high requirements for equipment and reagents. Ultimately, when the technology is implemented, laboratories often face the dilemma of "either increasing workload, increasing costs, or sacrificing the reliability of results".

[0062] In contrast, the auxiliary positioning and template scheme proposed in this utility model can ensure accurate positioning in experiments, is easy to operate, and has controllable costs. Example 2

[0063] Based on Example 1, this invention proposes the preparation and use of a laser-etched glass sample.

[0064] Material preparation: Select a high-strength borosilicate glass plate with a thickness of 2.5mm, a size of 80mm×100mm, and a surface finish Ra<0.1μm.

[0065] Structural processing: Using laser etching equipment (model HGX-355 can be used), the sample marking frame and letter number are engraved on the inside of the glass plate. At the same time, the tooth protrusion 101 structure is processed using precision CNC micro-milling technology. The tooth protrusion 101 has a height of 0.3mm and a width of 0.5mm, and its position is aligned with the tooth groove 302 of the standard rubber comb 3.

[0066] The line width of the identification frame 102 is controlled at 0.2mm, the engraving depth is 0.05mm, and the letter numbering range is from A to O.

[0067] Usage steps:

[0068] (1) Install the sample loading main board 1 with the etched surface facing the direction of the glue-making tank, forming a gel interlayer with the bottom and sides sealed between the sample loading sub-board 2 and the glue-making frame;

[0069] (2) After adding the adhesive to the interlayer, insert the adhesive comb 3 so that the tooth protrusion 101 engages with the tooth groove 302 of the adhesive comb 3;

[0070] (3) After the adhesive solidifies into a colloid, take out the adhesive comb 3 and form a sample loading hole on the colloid corresponding to the comb teeth 301 of the adhesive comb 3.

[0071] (4) When adding samples to the sample well, refer directly to the marking line and number in the label box 102.

[0072] Effect observation:

[0073] In multiple sample addition experiments, the positioning error was ≤ ±0.1mm, the numbering was clear, and it could withstand more than 50 high-pressure washings without fading. The operation time was reduced by an average of 28% compared to unmarked glass plates.

[0074] In this embodiment, the toothed protrusion 101 and the sample loading main plate 1 adopt an integral molding structure, which can ensure the overall structural strength and stability of the sample loading plate. Example 3

[0075] Based on Example 1, this utility model also proposes an alternative to the preparation of structural glass and templates (screen printing coating scheme).

[0076] Material preparation: The sample motherboard 1 uses a tempered glass plate of the same specification (2.8mm thick), and the surface is cleaned with deionized water.

[0077] Structural processing: The sample frame lines and numbers are printed using screen printing (ink: corrosion-resistant epoxy ink). The curing temperature is 150℃ and the time is 10 minutes. The line width of the marking frame 3 is 0.3mm. The serrations are made of transparent silicone strips with a thickness of 0.3mm and a width of 0.5mm, which are fixed to the edge of the glass plate with polymer adhesive.

[0078] Usage testing: After gel preparation and sample loading tests, the positioning error was approximately ±0.2mm; the silkscreen markings remained clear after 80 cleanings, making them suitable for medium-frequency experimental scenarios; the adhesion strength of the 101 teeth was good and they can be replaced.

[0079] In this embodiment, the toothed protrusion 101 is detachably installed on the inner side of the sample loading main board 1. That is, the sample loading main board 1 and the toothed protrusion 101 adopt a detachable split structure, which can achieve the same positioning effect as the integrated structure. Moreover, the split toothed protrusion 101 is easier to replace to adapt to different sample loading hole sizes and numbers, thereby improving the applicability of the device. Example 4

[0080] Based on the above embodiments, this utility model also conducted a comparative experiment on multi-parameter optimization design, with the aim of evaluating the impact of different structural schemes on the accuracy and repeatability of sample addition.

[0081] The specific experimental details are shown in the table below:

[0082]

[0083] Results analysis:

[0084] Example 2 performs best in terms of positioning accuracy, operational efficiency, and reusability;

[0085] Example 3 has a lower cost and is suitable for low- to medium-frequency applications;

[0086] Traditional methods have large localization errors, are prone to sample confusion, and have poor repeatability.

[0087] The testing instruments used in the above-mentioned tests may be, but are not limited to, the following models:

[0088] Etching accuracy was tested using a three-dimensional laser scanning microscope (KEYENCEVK-X200).

[0089] The cleaning durability test was conducted using a high-pressure water jet flushing machine (0.5 MPa).

[0090] The sample loading error test was performed using a gel imaging system with a scale (Bio-Rad ChemiDocMP) to analyze the deviation in sample loading position.

[0091] In summary, the Western blot glass sample loading plate proposed in this invention, through its innovative structural design, guides the sample loading process, significantly improving the standardization of experimental operations and the reliability of sample management. The above embodiments have been repeatedly verified in a laboratory environment, demonstrating strong technical feasibility and good potential for widespread application.

[0092] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0093] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0094] Based on the preferred embodiments of this utility model described above, those skilled in the art can make various changes and modifications without departing from the technical concept of this utility model. The technical scope of this utility model is not limited to the contents of the specification, but must be determined according to the scope of the claims.

Claims

1. An auxiliary positioning template, characterized in that: The sample dispensing board (1) is provided with a tooth protrusion (101) on one side for embedding in the corresponding tooth groove (302) of the glue-making comb (3). The tooth protrusion (101) is distributed at equal intervals along a straight line at the edge of the sample dispensing area on the upper part of the sample dispensing board (1), and the comb teeth (301) of the glue-making comb (3) are embedded in the gap between two adjacent tooth protrusions (101).

2. The auxiliary positioning template according to claim 1, characterized in that: The toothed protrusion (101) and the sample loading main plate (1) adopt an integral molding structure.

3. The auxiliary positioning template according to claim 1, characterized in that: The toothed protrusion (101) is detachably installed on the inside of the sample application main plate (1).

4. The auxiliary positioning template according to any one of claims 1-3, characterized in that: The sample loading motherboard (1) is made of borosilicate glass or tempered glass.

5. The auxiliary positioning template according to claim 4, characterized in that: The sample loading main board (1) is provided with an identification frame (102) for marking the outline and position of the sample loading hole, and the identification frame (102) has a U-shaped structure.

6. The auxiliary positioning template according to claim 5, characterized in that: The identification frame (102) is provided with a number below it, and the number corresponds one-to-one with the position of the sample loading hole.

7. The auxiliary positioning template according to claim 6, characterized in that: The identification frame (102) and the number are both located on the inner side of the sample addition motherboard (1).

8. The auxiliary positioning template according to claim 1, characterized in that: The sample loading main plate (1) has a length, width, and thickness of 100mm, 80mm, and 2-3mm, respectively, and the tooth protrusion (101) has a height and width of 0.3mm and 0.5mm, respectively.

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