Cell culture frame

Abstract

The utility model relates to the field of cell culture, and discloses a cell culture shelf which comprises a detection system for analyzing components of a culture solution, a culture system for culturing cells and a microscopic system for observing cell states, and the culture system is connected with the detection system through a transmission system; the culture system comprises a liquid storage cylinder connected with the transmission system, a liftable culture box is arranged in the liquid storage cylinder, and a separation plate is arranged at the bottom of the culture box. A culture solution is injected into a liquid storage cylinder of the culture system, and the culture solution can enter a culture box through a separation plate to realize cell culture; the culture solution can also enter the detection system through the transmission system for component analysis, so that the pollution problem in the process can be avoided; the culture box can be lifted, so that the culture solution is convenient to replace; the device is simple in structure and convenient to operate, and can effectively solve the problems of culture solution replacement, component detection and real-time observation in the cell culture process.

Description

Technical Field

[0001] This utility model relates to the field of cell culture, specifically to a cell culture rack. Background Technology

[0002] In cell biology and medical research, cell culture, as a fundamental experimental technique, is widely used in fields such as cell biology, drug screening, and tissue engineering. Cell culture racks are key tools in the laboratory, used to provide the support and environment required for cell growth, ensuring the smooth progress of cell growth, division, and subsequent experimental procedures.

[0003] For example, Chinese Patent Publication No. CN221254570U, published on July 2, 2024, discloses a novel cell comparison detection device, including a device body with inlets on both sides. The front and rear sides of the bottom of the inner wall of the device body are provided with clamping and easily replaceable structures. A culture dish is disposed inside the clamping and easily replaceable structures, and a structure for maintaining stability is provided inside the clamping and easily replaceable structures. This device can conveniently detect multiple cell culture dishes. Its advantage lies in solving the problem in existing technologies where cell sample culture dishes are difficult to remove from the detection range of an electron microscope, while also facilitating the replacement of different cell sample culture dishes.

[0004] However, the aforementioned device cannot avoid contamination problems during the process of placing the culture dish into the detection device; at the same time, the device can only be observed and detected through a microscope, and cannot meet the needs of real-time observation during cell growth; therefore, there is an urgent need for a new technical solution to effectively solve the problems of changing culture medium, detecting components, and real-time observation during cell culture. Utility Model Content

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a cell culture rack to effectively solve the problems of changing culture medium, detecting components, and real-time observation during cell culture.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows: a cell culture rack, comprising a detection system for analyzing the components of the culture medium, a culture system for culturing cells, and a microscopic system for observing the cell state. The microscopic system is located above the culture system, the culture system is located above the detection system, and the culture system and the detection system are connected by a transmission system. The culture system includes a liquid storage cylinder connected to the transmission system, and a culture box that can be raised and lowered is provided inside the liquid storage cylinder. A separation plate for separating the culture medium and cells is provided at the bottom of the culture box.

[0007] Optionally, the culture box includes a box body, the bottom of which is connected to a culture section for culturing cells, and the separation plate is installed at the bottom of the culture section.

[0008] Optionally, a first connecting block is installed on the top of the liquid storage cylinder, and a second connecting block corresponding to the position and number of the first connecting block is installed on the top of the box body, and the corresponding first connecting block and second connecting block are connected by a connecting rope.

[0009] Optionally, a partition is installed on the inner side of the culture section, and the partition divides the inner cavity of the culture section into two independent chambers.

[0010] Optionally, the transmission system includes a transmission conduit for connecting the liquid storage cylinder and the detection system, with a liquid-blocking plate rotatably mounted at the end of the transmission conduit for blocking or connecting the transmission conduit, and a second adjustment knob for driving the liquid-blocking plate to rotate is mounted on the outside of the transmission conduit.

[0011] Optionally, the detection system includes a housing, on which a detector for detecting the components of the culture medium is mounted. A liquid guide tube connected to the transmission conduit is mounted on the top of the housing. A detection conduit is connected to the side of the liquid guide tube, and the end of the detection conduit opposite to the liquid guide tube is connected to the detector.

[0012] Optionally, a waste liquid cylinder for collecting culture medium is provided below the liquid guide tube, and the waste liquid cylinder can be detachably installed inside the housing.

[0013] Optionally, a display screen for displaying detection results is mounted on the outside of the detector, and the data display surface of the display screen is located on the outside of the housing.

[0014] Optionally, the microscope system includes a connecting plate, on one side of which a microscope tube is mounted and on the other side which a plurality of objective lenses are mounted. An eyepiece is mounted at the end of the microscope tube, and the microscope tube is connected to the housing via a support system.

[0015] Optionally, the support system includes a base for supporting the housing, a support rod fixedly mounted on the base, an adjustable rod capable of being raised and lowered mounted on the support rod, and the end of the adjustable rod being fixedly connected to the lens barrel; wherein, the support rod is perpendicular to the base, the adjustable rod is perpendicular to the support rod, and a first knob for driving the adjustable rod to be raised and lowered is also mounted on the support rod.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0017] (1) In this utility model, the culture medium is injected into the storage tank of the culture system. The culture medium can enter the culture box through the separation plate to realize cell culture. The culture medium can also enter the detection system through the transmission system for component analysis, which can avoid the pollution problem in the process. The culture box can be raised and lowered to facilitate the replacement of culture medium. The device has a simple structure and is easy to operate. It can effectively solve the problems of culture medium replacement, component detection and real-time observation in the cell culture process.

[0018] (2) For the above detection system, the culture medium can enter the built-in detector through the detection tube with the side opening of the liquid guide tube, and analyze the metabolites and waste components in the liquid in real time, providing accurate environmental monitoring and data support for the experiment;

[0019] (3) In this utility model, the efficient waste liquid cleaning system, which consists of the connecting block, connecting rope, culture box and liquid storage tank in the culture system and the transmission conduit, liquid blocking plate and second adjustment knob in the transmission system, can remove metabolic waste and attachments generated by cells during the culture process in real time and optimize the cell growth environment.

[0020] (4) In this utility model, the integrated design of the culture rack and the microscope system not only simplifies the experimental operation, but also avoids the frequent replacement and accuracy problems caused by the separate use of traditional cell culture racks and microscopes. Users can conduct microscopic observation of cells in real time and conveniently. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of the cell culture rack in an embodiment of this utility model;

[0022] Figure 2 This is a side view of the cell culture rack in an embodiment of the present invention;

[0023] Figure 3 This is a schematic diagram of the structure of the microscopic system in an embodiment of this utility model;

[0024] Figure 4 This is a schematic diagram of the transmission system in an embodiment of this utility model;

[0025] Figure 5 This is a schematic diagram of the structure of the culture box in an embodiment of this utility model;

[0026] Figure 6 This is a schematic diagram of the detection system in an embodiment of this utility model;

[0027] Wherein, 1a, base; 1b, support rod; 1c, first adjustment knob; 1d, adjustment rod;

[0028] 2. Detection system; 2a. Detector; 2a1. Display screen; 2b. Waste liquid tank; 2c. Detection conduit; 2d. Liquid guide tube;

[0029] 3. Transmission system; 3a. Transmission conduit; 3b. Liquid-blocking plate; 3c. Second adjustment knob;

[0030] 4a. Storage tank; 4b. Culture box; 4b1. Separation plate; 4b2. Partition; 4b3. Culture section; 4b4. Box body; 4c1. Lower left connecting block; 4c2. Lower right connecting block; 4c3. Left connecting rope; 4c4. Right connecting rope; 4c5. Upper left connecting block; 4c6. Upper right connecting block;

[0031] 5a. Connecting plate; 5b1. 4x scope; 5b2. 10x scope; 5b3. 40x scope; 5c. Scope tube; 5c1. Eyepiece. Detailed Implementation

[0032] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. These drawings are simplified schematic diagrams, which are only used to illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0033] like Figure 1 and Figure 2 As shown, a cell culture rack includes a support system, a detection system 2, a transport system 3, a culture system, and a microscopic system. The support system is used to connect the microscopic system and the detection system 2, the transport system 3 is used to connect the detection system 2 and the culture system, the detection system 2 is used to analyze the composition of the culture medium, the culture system is used for cell culture, and the microscopic system is used to observe the growth status of the cells.

[0034] As described above, the microscopic system is located above the culture system 2, which in turn is located above the detection system 2. The culture system and the detection system 2 are connected via a transmission system 3. This integrated design of the culture rack and microscopic system not only simplifies experimental operations but also avoids the frequent switching and accuracy issues associated with using separate cell culture racks and microscopes in traditional methods. Users can perform real-time and convenient microscopic observation of cells.

[0035] like Figure 2 and Figure 5 As shown, the culture system includes a storage tank 4a connected to the transfer system 3. Inside the storage tank 4a is a culture box 4b that can be raised and lowered. At the bottom of the culture box 4b is a separation plate 4b1 for separating the culture medium and cells. When the culture medium is injected into the storage tank 4a, it can flow from bottom to top into the culture box 4b through the separation plate 4b1 to achieve cell culture.

[0036] As described above, the culture box 4b includes a box body 4b4, a culture section 4b3, and a separation plate 4b1 connected sequentially from top to bottom. That is, the bottom of the box body 4b4 is connected to the culture section 4b3 for culturing cells, and the separation plate 4b1 is installed at the bottom of the culture section 4b3.

[0037] A first connecting block is installed on the top of the liquid storage cylinder 4a, and a second connecting block corresponding to the position and number of the first connecting block is installed on the top of the box body 4b4, and the corresponding first connecting block and second connecting block are connected by a connecting rope.

[0038] A partition 4b2 is installed on the inner side of the culture section 4b3, dividing the inner cavity of the culture section 4b3 into two independent chambers. The surfaces of the culture box 4b, except for the bottom, the reservoir 4a, and the partition 4b2 can all be made of polystyrene (PS). The separation plate 4b1 can be made of polyethersulfone (PES), which has good barrier properties, effectively isolating cells, and has high permeability to cell culture medium and small molecule products. The cell culture section 4b3 of the culture box 4b is divided into two parts by the partition 4b2, thus allowing for the simultaneous culture and comparative observation of two batches of cells.

[0039] Specifically, the upper edge of the upper box 4b4 is tightly connected to the two first connecting blocks with strong glue. The middle culture section 4b3 is divided into two parts by the centrally erected partition 4b2. The partition 4b2 is embedded in the groove inside the culture section 4b3 and can be disassembled. The lower separation plate 4b1 is the bottom of the culture box 4b.

[0040] The first connecting block consists of two parts: the upper left connecting block 4c5 and the upper right connecting block 4c6. The length of the line connecting the two parts is the diameter of the top circle of the liquid storage cylinder 4a. They are tightly connected to the liquid storage cylinder 4a with strong adhesive and are adjacent to the two connecting blocks below, meaning that the two pairs of connecting blocks are closest to each other. The second connecting block consists of two parts: the lower left connecting block 4c1 and the lower right connecting block 4c2. The length of the line connecting the two parts is the diameter of the top circle of the culture box 4b. They are tightly connected to the culture box 4b with strong adhesive.

[0041] Similarly, there are two connecting ropes, namely the left connecting rope 4c3 and the right connecting rope 4c4. The two connecting ropes are respectively emitted from the two first connecting blocks, and the ends of the ropes are fixed to the two upper first connecting blocks. By rotating the two lower second connecting blocks, the length of the ropes can be adjusted, thereby realizing the function of raising and lowering the culture box 4b.

[0042] When the culture medium needs to be changed, rotate the lower left connecting block 4c1 and the lower right connecting block 4c2 to shorten the lengths of the left connecting rope 4c3 and the right connecting rope 4c4. This allows the culture box 4b to move upward, and the culture medium flows out naturally through the separation plate 4b1, remaining in the reservoir 4a. The separation plate 4b1, carrying the cultured cells, moves upward and separates from the culture medium. After the culture medium has completely flowed out of the reservoir 4a, new culture medium can be added through the funnel between the reservoir 4a and the culture box 4b.

[0043] like Figure 2 and Figure 4 As shown, the transmission system 3 includes a transmission conduit 3a for connecting the liquid storage cylinder 4a and the detection system 2. A liquid blocking plate 3b for blocking or connecting the transmission conduit 3a is rotatably installed at the end of the transmission conduit 3a, and a second adjustment knob 3c for driving the liquid blocking plate 3b to rotate is installed on the outside of the transmission conduit 3a.

[0044] The transmission conduit 3a in the transmission system 3 is connected to the upper liquid storage cylinder 4a through the opening in the center of the bottom of the liquid storage cylinder 4a, and the two are tightly connected; at the same time, the transmission conduit 3a is also connected to the lower detection system 2; a liquid blocking plate 3b is provided in the transmission conduit 3a close to the upper liquid storage cylinder 4a.

[0045] The liquid blocking plate 3b is used to control the opening and closing of the transmission conduit 3a, and its radius is the same as that of the transmission conduit 3a; the second adjusting knob 3c is located on the outer wall of the transmission conduit 3a, and its head passes through the transmission conduit 3a and is located at the same horizontal position as the liquid blocking plate 3b inside, and is tightly connected; there is no gap between the head of the second adjusting knob 3c and the transmission conduit 3a, and it is fixed to the wall of the transmission conduit 3a by screws; the liquid blocking plate 3b can be controlled by the second adjusting knob 3c, and rotates in the horizontal direction with the head of the second adjusting knob 3c as the base point.

[0046] Specifically, the cell culture rack has an internal liquid channel; the liquid-blocking plate 3b in the transfer conduit 3a acts as a switch. When the second adjustment knob 3c on the transfer conduit 3a is rotated to move the liquid-blocking plate 3b away, the switch is turned on, and the liquid can be naturally transferred from the reservoir 4a to the detector 2a through the transfer conduit 3a. When the liquid-blocking plate 3b is completely in the transfer conduit 3a, the liquid cannot flow, and the switch is turned off.

[0047] The transmission conduit 3a, the liquid conduit 2d, and the detection conduit 2c can all be made of polytetrafluoroethylene (PTFE), while the liquid blocking sheet 3b can be made of thermoplastic elastomer (TPE) to improve its sealing performance and durability.

[0048] like Figure 1 , Figure 2 and Figure 6As shown, the detection system 2 includes a housing, on which a detector 2a for detecting the components of the culture medium is installed. A liquid guide tube 2d connected to a transmission conduit 3a is installed on the top of the housing. A detection conduit 2c is connected to the side of the liquid guide tube 2d, and the end of the detection conduit 2c opposite to the liquid guide tube 2d is connected to the detector 2a. A display screen 2a1 for displaying the detection results is installed on the outside of the detector 2a, and the data display surface of the display screen 2a1 is located on the outside of the housing.

[0049] As described above, a short vertical liquid guide tube 2d is connected to the central opening at the top of the housing of the detection system 2, and is tightly connected to the upper transmission conduit 3a; a detection conduit 2c is provided on the side of the liquid guide tube 2d to facilitate the introduction of liquid into the detector 2a for component analysis.

[0050] The detector 2a is located close to the housing and serves as its display screen 2a1. An opening is formed at the outermost junction of the housing and the display screen 2a1, exposing the data display surface of the display screen 2a1. The detector 2a is inserted into the housing via two slots on the inside, making it a detachable structure.

[0051] Furthermore, a waste liquid cylinder 2b for collecting culture medium is provided below the liquid guide tube 2d, and the waste liquid cylinder 2b can be detachably installed inside the shell; the top opening of the waste liquid cylinder 2b is connected to the liquid guide tube 2d but not tightly connected, the front side of the waste liquid cylinder 2b is close to the detector 2a, and the left, lower and rear sides are close to the shell respectively.

[0052] To facilitate the disassembly and assembly of the waste liquid cylinder 2b, the right side of the housing is an open side, allowing for quick removal or insertion of the waste liquid cylinder 2b from or into the housing. The waste liquid cylinder 2b can be made of polyvinyl chloride (PVC), while the housing can be made of high-density polyethylene (HDPE) to enhance its corrosion resistance and durability.

[0053] The efficient waste liquid cleaning system, consisting of the connecting block, connecting rope, culture box 4b, and liquid storage tank 4a in the culture system, as well as the transfer conduit 3a, liquid blocking plate 3b, and second adjustment knob 3c in the transfer system 3, can remove metabolic waste and attachments generated by cells during the culture process in real time, thus optimizing the cell growth environment.

[0054] When the culture medium needs to be replaced, the second adjusting knob 3c on the transmission conduit 3a can be rotated to partially remove the liquid blocking plate 3b, allowing the culture medium to flow into the detector 2a along the transmission conduit 3a. The detection conduit 2c on the side of the liquid guide tube 2d in the detection system 2 guides the liquid into the detector 2a. The detector 2a can be selected to perform a detection, and the detection result is displayed on the display screen 2a1 on the detector 2a. At the same time, most of the liquid flows into the waste liquid tank 2b through the liquid guide tube 2d. The culture medium can be completely removed by periodically replacing the waste liquid tank 2b in the detection system 2.

[0055] like Figure 1 , Figure 2 and Figure 3 As shown, the microscope system includes a connecting plate 5a, a microscope tube 5c is mounted on one side of the connecting plate 5a, and multiple objectives are mounted on the other side. An eyepiece 5c1 is mounted at the end of the microscope tube 5c, and the microscope tube 5c is connected to the housing through a support system.

[0056] The microscope's connecting plate 5a can be rotated to switch between different objectives. Three objectives are available: a 4x objective 5b1, a 10x objective 5b2, and a 40x objective 5b3. Users can choose the appropriate objective according to their needs. The objectives are tightly connected to the upper connecting plate 5a, and the centers of the three objectives form an equilateral triangle, with the geometric center of the triangle being the center of the base of the connecting plate 5a. The connecting plate 5a is tightly connected to the microscope tube 5c located at the center of its top surface. The eyepiece 5c1 is installed at the top of the microscope tube 5c, and the two are detachably and tightly connected by threads.

[0057] The aforementioned support system includes a base 1a for supporting the housing, a support rod 1b fixedly mounted on the base 1a, an adjustable rod 1d capable of being raised and lowered mounted on the support rod 1b, and the end of the adjustable rod 1d fixedly connected to the lens barrel 5c, that is, the middle part of the outer side of the lens barrel 5c is fixedly connected to the adjustable rod 1d by strong glue.

[0058] Among them, the support rod 1b is perpendicular to the base 1a, the adjusting rod 1d is perpendicular to the support rod 1b, and the support rod 1b is also equipped with a first adjusting knob 1c for driving the adjusting rod 1d to rise and fall; the first adjusting knob 1c and the connecting plate 5a can both be made of stainless steel to ensure their durability and stability.

[0059] The integrated design of the culture rack and microscope system not only simplifies experimental operations but also avoids the frequent switching and accuracy problems caused by the separate use of traditional cell culture racks and microscopes. Users can conduct real-time and convenient microscopic observation of cells.

[0060] When using this cell culture rack, first check the second adjustment knob 3c in the transfer system 3 to ensure that the liquid-blocking plate 3b is completely inside the transfer tube 3a; then, use a funnel to inject the cell culture medium into the storage tank 4a through the gap between the culture box 4b and the storage tank 4a; subsequently, place the cells to be cultured at the bottom of the culture box 4b, i.e., on the separation plate 4b1.

[0061] During cell culture, the cell state can be observed in real time using a microscope system. By rotating the first adjustment knob 1c in the support system, the microscope tube 5c and the connecting plate 5a tightly connected to it move up and down, thereby adjusting the three objective lenses up and down until the image is clear; at this point, tighten the first adjustment knob 1c to fix the position for observation.

[0062] The lifting principle of the connecting plate 5a and the microscope tube 5c is the same as that of the coarse and fine focus adjustment knobs in existing microscopes. They are connected by a gear and rack transmission device. When the first adjustment knob 1c is turned, the gear connected to it will rotate. Since the gear and rack mesh with each other, the rack will drive the microscope tube 5c and the connecting plate 5a to move up and down through the adjustment rod 1d, thereby completing the focus adjustment and allowing the user to see the image in the eyepiece 5c1. Since the principle is the same as that of the prior art, its internal structure is not shown.

[0063] During cell culture, cell metabolites can be monitored in real time. By rotating the second adjustment knob 3c in the transmission system 3, the liquid-blocking plate 3b is rotated horizontally. After some cell culture medium flows out, the second adjustment knob 3c is adjusted again to return the liquid-blocking plate 3b to its original position. The cell culture medium enters the liquid guide tube 2d in the detection system 2 through the misaligned gap between the transmission conduit 3a and the liquid-blocking plate 3b, and then enters the detector 2a for analysis through the branch on the side of the liquid guide tube 2d (i.e., the detection conduit 2c). Specific operations and data display can be completed through the display screen 2a1 exposed on the side of the detection system 2.

[0064] When the culture medium needs to be changed, the lower left connecting block 4c1 and the lower right connecting block 4c2 can be rotated to adjust the length of the left connecting rope 4c3 and the right connecting rope 4c4, causing the culture box 4b inside the storage tank 4a to move upward. Both the lower left connecting block 4c1 and the lower right connecting block 4c2 are cable reeling and unloading devices. Their outer shells can be made of stainless steel, and the internal connecting ropes can be made of ultra-high molecular weight polyethylene fiber (UHMWPE) to enhance flexibility and corrosion resistance.

[0065] Cells in culture section 4b3 are blocked by separation plate 4b1 and move upward with culture box 4b, while cell culture medium remains in reservoir 4a through separation plate 4b1, thus achieving separation of cells and cell culture medium. Afterwards, the adjustment knob in transfer system 3 is rotated to remove the liquid-blocking plate 3b, allowing the cell culture medium to enter detection system 2 through transfer conduit 3a.

[0066] Most of the liquid is guided to the waste liquid tank 2b through the liquid guide tube 2d. After the cell culture medium has completely flowed out of the storage tank 4a, the second adjusting knob 3c is turned to reset the liquid-blocking plate 3b. At this time, the user can inject new culture medium into the storage tank 4a again through the funnel.

[0067] By rotating the lower left connecting block 4c1 and the lower right connecting block 4c2, some of the connecting ropes are released, extending the length of the left connecting rope 4c3 and the right connecting rope 4c4. This causes the culture box 4b to descend, ensuring that the culture section 4b3 is completely immersed in the new culture medium. If necessary, the above steps can be repeated to thoroughly clean the separation plate 4b1 and any residual cell culture medium on the cell surface.

[0068] When the waste liquid tank 2b is nearly full, it can be pulled out through the right side of the detection system 2, the waste cell culture medium inside can be poured out, and the waste liquid tank 2b can be put back into its original position.

[0069] Furthermore, when cleaning detector 2a is required, the user can pull out detector 2a, which is stuck in the slot, through the right side of detection system 2 for cleaning. If detector 2a needs to be replaced (e.g., by a flow cytometer, enzyme-linked immunosorbent assay (ELISA) instrument, or high-performance liquid chromatography (HPLC) instrument), the old detector 2a can also be pulled out through the right side of detection system 2 and the new detector 2a installed, while ensuring that display screen 2a1 can be exposed through the opening on the side of detection system 2.

[0070] In summary, this invention provides an integrated and efficient cell culture solution that enables cell culture, observation, detection, and cleaning operations to be performed on the same platform. This solution not only improves the convenience and safety of experimental operations but also significantly reduces the risk of contamination during cell culture.

[0071] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0072] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0073] Based on the preferred embodiments of this utility model described above, those skilled in the art can make various changes and modifications without departing from the technical concept of this utility model. The technical scope of this utility model is not limited to the contents of the specification, but must be determined according to the scope of the claims.

Claims

1. A cell culture rack, characterized in that: The system includes a detection system (2) for analyzing the composition of the culture medium, a culture system for culturing cells, and a microscopic system for observing the cell state. The microscopic system is located above the culture system, and the culture system is located above the detection system (2). The culture system and the detection system (2) are connected by a transmission system (3). The culture system includes a reservoir (4a) connected to the transmission system (3). The reservoir (4a) has a culture box (4b) that can be raised and lowered inside. The bottom of the culture box (4b) has a separation plate (4b1) for separating the culture medium and cells.

2. The cell culture rack according to claim 1, characterized in that: The culture box (4b) includes a box body (4b4), the bottom of which is connected to a culture section (4b3) for culturing cells, and the separation plate (4b1) is installed at the bottom of the culture section (4b3).

3. The cell culture rack according to claim 2, characterized in that: A first connecting block is installed on the top of the liquid storage cylinder (4a), and a second connecting block corresponding to the position and number of the first connecting block is installed on the top of the box body (4b4), and the corresponding first connecting block and second connecting block are connected by a connecting rope.

4. The cell culture rack according to claim 3, characterized in that: A partition (4b2) is installed on the inner side of the culture section (4b3), and the partition (4b2) divides the inner cavity of the culture section (4b3) into two independent chambers.

5. The cell culture rack according to claim 1, characterized in that: The transmission system (3) includes a transmission conduit (3a) for connecting the liquid storage cylinder (4a) and the detection system (2). The end of the transmission conduit (3a) is rotatably equipped with a liquid blocking plate (3b) for blocking or connecting the transmission conduit (3a), and a second adjustment knob (3c) for driving the liquid blocking plate (3b) to rotate is installed on the outside of the transmission conduit (3a).

6. The cell culture rack according to claim 5, characterized in that: The detection system (2) includes a housing, on which a detector (2a) for detecting the components of the culture medium is installed. A liquid guide tube (2d) connected to the transmission conduit (3a) is installed on the top of the housing. A detection conduit (2c) is connected to the side of the liquid guide tube (2d), and the end of the detection conduit (2c) opposite to the liquid guide tube (2d) is connected to the detector (2a).

7. The cell culture rack according to claim 6, characterized in that: Below the liquid guide tube (2d) is a waste liquid cylinder (2b) for collecting culture medium, and the waste liquid cylinder (2b) can be detachably installed inside the shell.

8. The cell culture rack according to claim 7, characterized in that: The detector (2a) is equipped with a display screen (2a1) for displaying detection results on its outer side, and the data display surface of the display screen (2a1) is located on the outer side of the housing.

9. The cell culture rack according to claim 8, characterized in that: The microscopic system includes a connecting plate (5a), on one side of which a microscope tube (5c) is mounted, and on the other side which a plurality of objective lenses are mounted. An eyepiece (5c1) is mounted at the end of the microscope tube (5c), and the microscope tube (5c) is connected to the housing via a support system.

10. The cell culture rack according to claim 9, characterized in that: The support system includes a base (1a) for supporting the housing, a support rod (1b) fixedly installed on the base (1a), an adjustable rod (1d) capable of being raised and lowered installed on the support rod (1b), and the end of the adjustable rod (1d) is fixedly connected to the lens barrel (5c). The support rod (1b) is perpendicular to the base (1a), the adjusting rod (1d) is perpendicular to the support rod (1b), and a first knob for driving the adjusting rod (1d) to rise and fall is also installed on the support rod (1b).

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