Bioreactor for stem cell exosome culture

Abstract

The utility model relates to the technical field of exosome preparation, in particular to a bioreactor for stem cell exosome culture, which comprises a working table, a control panel is fixedly connected to the front surface of the working table, a rotating disc is mounted on the top surface of the working table, a driving motor is arranged at the lower end of the rotating disc, and the driving motor is connected with the control panel. And the driving motor is mounted in the workbench, a mounting column and a positioning rod are sequentially and fixedly connected to the top face of the rotating disc from left to right, and a mounting base is mounted on the top face of the mounting column and the top face of the positioning rod. According to the cell culture device, the mounting base and the culture cylinder can be driven to rotate stably through the rotating disc, and then the mounting motor is started to drive the rotating shaft and the stirring assembly to rotate, so that a culture solution and cells in the culture cylinder can be uniformly rotated through the design of the rotating shaft and the stirring assembly; therefore, the culture solution and the cells can be uniformly mixed.

Description

Technical Field

[0001] This utility model relates to the field of exosome preparation technology, specifically a bioreactor for culturing stem cell exosomes. Background Technology

[0002] Stem cell exosomes are tiny vesicles, 40-100 mm in diameter, that can transfer biomolecules such as lipids, carbohydrates, proteins, mRNA, miRNA, and DNA from one cell to another, thereby exchanging genetic information, reprogramming the host cell, and facilitating intercellular communication. Stem cell exosomes possess important biological functions such as reducing apoptosis, alleviating inflammation, promoting angiogenesis, inhibiting fibrosis, and enhancing tissue repair potential.

[0003] In response, Chinese patent application number CN219156893U discloses a bioreactor for stem cell exosome culture, comprising a cell culture chamber and a base. A gear ring is fixedly mounted on the base, and a first gear and a drive mechanism for driving the first gear are rotatably mounted within the gear ring on the base. The rotation axis of the first gear is aligned with the height direction of the base. A second gear is rotatably mounted between the gear ring and the first gear, and the second gear meshes with both the first gear and the gear ring. The cell culture chamber is fixedly mounted on the second gear. This application has the effect of improving the inadequate mixing of culture medium and cells.

[0004] However, in actual use, the culture chamber can only be rotated by external rotational power, which cannot evenly mix the culture medium and cells inside. At the same time, the culture medium also needs to be evenly distributed when it is added. Therefore, improving and perfecting the above-mentioned problems has become an urgent issue to be solved. Utility Model Content

[0005] The purpose of this invention is to provide a bioreactor for stem cell exosome culture, in order to solve the problems mentioned in the background art, where the culture chamber can only be rotated by external rotational power, making it impossible to uniformly mix the culture medium and cells inside, and the culture medium also needs to be uniformly added when it is added.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a bioreactor for stem cell exosome culture, comprising a workbench, a control panel fixedly connected to the front surface of the workbench, a rotating disk mounted on the top surface of the workbench, a drive motor provided at the lower end of the rotating disk, the drive motor being installed inside the workbench, and mounting columns and positioning rods fixedly connected sequentially from left to right on the top surface of the rotating disk, with mounting bases mounted on the top surfaces of the mounting columns and positioning rods.

[0007] Preferably, the lower end of the mounting base is provided with a groove that is compatible with the mounting column and the positioning rod, and a culture tube is mounted on the top surface of the mounting base.

[0008] Preferably, a top plate is installed on the top surface of the inner wall of the culture tube, and a motor and a feed tube are installed sequentially from left to right on the top surface of the top plate.

[0009] Preferably, the output end of the motor is mounted with a rotating shaft that penetrates the top surface of the top plate, and a stirring assembly is mounted on the outer surface of the rotating shaft.

[0010] Preferably, a solenoid valve is installed on the outer surface of the feed cylinder, and a discharge pipe is installed at the lower end of the feed cylinder through the top surface of the top plate.

[0011] Preferably, a sealing plug is installed inside the feed cylinder, and a sealing cap is installed on the top surface of the sealing plug.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] 1. This bioreactor for stem cell exosome culture, through its rotating disk, drive motor, mounting column, positioning rod, mounting base, culture cylinder, top plate, mounting motor, feed cylinder, rotating shaft, and stirring assembly, operates as follows: When the culture medium and cells are inside the culture cylinder, the drive motor is first activated, causing the rotating disk to rotate. The mounting column and positioning rod then position the mounting base on the top surface of the rotating disk. The rotating disk enables the mounting base and culture cylinder to rotate stably. Activating the mounting motor then drives the rotating shaft and stirring assembly to rotate, ensuring uniform mixing of the culture medium and cells inside the culture cylinder. This results in a good overall mixing effect, demonstrating the practicality of the design.

[0014] 2. This bioreactor for stem cell exosome culture, equipped with a feed cylinder, solenoid valve, discharge pipe, sealing plug, and sealing cap, allows for the addition of culture medium into the feed cylinder during use. The feed cylinder is then sealed with the sealing plug and cap. The solenoid valve controls the discharge of the culture medium from the feed cylinder, which then flows through the discharge pipe into the culture cylinder to mix with the cells. As the discharge pipe falls, the rotating shaft and stirring components inside the culture cylinder are activated, and the rotating disk also drives the culture cylinder to rotate. This design allows for the precise dispensing of culture medium, resulting in better subsequent mixing and demonstrating the design's functionality. Attached Figure Description

[0015] Figure 1 This is a three-dimensional schematic diagram of the structure of this utility model;

[0016] Figure 2 This is a schematic diagram showing the disassembled structure of the rotating disk and culture tube of this utility model;

[0017] Figure 3 This is a schematic diagram showing the disassembled structure of the culture tube and top plate of this utility model;

[0018] Figure 4 This is a three-dimensional schematic diagram of the feed cylinder and discharge pipe structure of this utility model.

[0019] In the diagram: 1. Workbench; 2. Control panel; 3. Rotary disc; 4. Drive motor; 5. Mounting column; 6. Positioning rod; 7. Mounting base; 8. Culture cylinder; 9. Top plate; 10. Mounting motor; 11. Feed cylinder; 12. Rotating shaft; 13. Stirring assembly; 14. Solenoid valve; 15. Discharge pipe; 16. Sealing plug; 17. Sealing cover. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] Please see Figure 1-4 One embodiment provided by this utility model:

[0022] A bioreactor for stem cell exosome culture is disclosed in this application. The control panel 2, drive motor 4, mounting motor 10, and solenoid valve 14 used in this application are all commercially available products. Their principles and connection methods are well-known prior art and will not be described in detail here. The bioreactor includes a worktable 1, with the control panel 2 fixedly connected to its front surface. A rotating disk 3 is mounted on the top surface of the worktable 1, and a drive motor 4 is located at the lower end of the rotating disk 3. The drive motor 4 is installed inside the worktable 1. From left to right, mounting posts 5 and positioning rods 6 are fixedly connected to the top surface of the rotating disk 3. A mounting base 7 is installed on the top surface of the container. The lower end of the mounting base 7 has a groove that matches the mounting column 5 and the positioning rod 6. A culture cylinder 8 is installed on the top surface of the mounting base 7. A top plate 9 is installed on the top surface of the inner wall of the culture cylinder 8. A motor 10 and a feed cylinder 11 are installed sequentially from left to right on the top surface of the top plate 9. A rotating shaft 12 is installed through the output end of the motor 10, penetrating the top surface of the top plate 9. A stirring assembly 13 is installed on the outer surface of the rotating shaft 12. A solenoid valve 14 is installed on the outer surface of the feed cylinder 11. A discharge pipe 15 is installed through the lower end of the feed cylinder 11, penetrating the top surface of the top plate 9. A sealing plug 16 is installed inside the feed cylinder 11. A sealing cap 17 is installed on the top surface of the 16. When the culture medium and cells are inside the culture tube 8, the drive motor 4 is first started, which drives the rotating disk 3 to rotate. Then, the mounting base 7 is positioned on the top surface of the rotating disk 3 by the design of the mounting column 5 and the positioning rod 6. The rotating disk 3 can drive the mounting base 7 and the culture tube 8 to rotate stably. Then, the mounting motor 10 is started, which drives the rotating shaft 12 and the stirring assembly 13 to rotate. Thus, the design of the rotating shaft 12 and the stirring assembly 13 can also rotate the culture medium and cells inside the culture tube 8 evenly, thereby making the culture medium and cells inside the culture tube 8 rotate evenly. The culture medium and cells are mixed evenly, and the overall mixing effect is good. The culture medium is added into the feed cylinder 11, and then the feed cylinder 11 is sealed with the sealing plug 16 and sealing cap 17. Then, the culture medium inside the feed cylinder 11 can be controlled by the solenoid valve 14 to feed the culture medium. Then, the culture medium will fall into the culture cylinder 8 through the feed pipe 15 to mix with the cells. When the feed pipe 15 falls, the rotating shaft 12 and the stirring component 13 inside the culture cylinder 8 are stirring and rotating. At the same time, the rotating disk 3 will also drive the culture cylinder 8 to rotate. This design can quantitatively add the culture medium, resulting in better subsequent mixing effect.

[0023] Working Principle: When using this device, the operator first connects it to an external power source to provide electrical support. When the culture medium and cells are inside the culture cylinder 8, the drive motor 4 is activated, causing the rotating disk 3 to rotate. The mounting column 5 and positioning rod 6 then position the mounting base 7 on the top surface of the rotating disk 3. The rotating disk 3 enables the mounting base 7 and the culture cylinder 8 to rotate stably. Next, the installation motor 10 is activated, driving the rotating shaft 12 and the stirring assembly 13 to rotate. The design of the rotating shaft 12 and the stirring assembly 13 also helps to rotate the culture medium and cells. The culture medium and cells inside the culture cylinder 8 are rotated evenly, thus ensuring uniform mixing. Culture medium is added into the feed cylinder 11, and then the feed cylinder 11 is sealed with the sealing plug 16 and sealing cap 17. Subsequently, the culture medium inside the feed cylinder 11 is fed out by the solenoid valve 14. This culture medium then falls into the culture cylinder 8 through the feed pipe 15 to mix with the cells. When the feed pipe 15 falls, the rotating shaft 12 and the stirring assembly 13 inside the culture cylinder 8 are stirring and rotating. At the same time, the rotating disk 3 also drives the culture cylinder 8 to rotate. The above is the working principle of this utility model.

[0024] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A bioreactor for culturing stem cell exosomes, comprising a workbench (1), wherein a control panel (2) is fixedly connected to the front surface of the workbench (1), characterized in that: The top surface of the workbench (1) is equipped with a rotating disk (3), and the lower end of the rotating disk (3) is provided with a drive motor (4). The drive motor (4) is installed inside the workbench (1). The top surface of the rotating disk (3) is fixedly connected with a mounting column (5) and a positioning rod (6) from left to right. The top surfaces of the mounting column (5) and the positioning rod (6) are equipped with mounting seats (7).

2. The bioreactor for stem cell exosome culture according to claim 1, characterized in that: The lower end of the mounting base (7) is provided with a groove that is compatible with the mounting column (5) and the positioning rod (6), and the top surface of the mounting base (7) is provided with a culture tube (8).

3. A bioreactor for stem cell exosome culture according to claim 2, characterized in that: The top surface of the inner wall of the culture tube (8) is equipped with a top plate (9), and the top surface of the top plate (9) is equipped with a motor (10) and a feed tube (11) from left to right.

4. A bioreactor for stem cell exosome culture according to claim 3, characterized in that: The output end of the motor (10) is mounted on a rotating shaft (12) through the top surface of the top plate (9), and a stirring assembly (13) is mounted on the outer surface of the rotating shaft (12).

5. A bioreactor for stem cell exosome culture according to claim 3, characterized in that: A solenoid valve (14) is installed on the outer surface of the feed cylinder (11), and a feed pipe (15) is installed at the lower end of the feed cylinder (11) through the top surface of the top plate (9).

6. A bioreactor for stem cell exosome culture according to claim 3, characterized in that: The inside of the feed cylinder (11) is fitted with a sealing plug (16), and the top surface of the sealing plug (16) is fitted with a sealing cap (17).

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