Cell tolerance testing device

Abstract

The utility model discloses a cell tolerance testing device, which belongs to the technical field of cell culture and comprises a shell, a switch door is arranged on one side of the shell, a liquid injection device is fixedly connected onto the shell, a receiving device and a rotating device are mounted in the shell, the liquid injection device comprises a first motor and an injection cylinder, and the first motor is connected with the rotating device. The output end of the first motor is fixedly connected with a piston, and the piston is arranged in the injection cylinder. According to the utility model, when virus inoculation liquid needs to be inoculated into cell liquid, the virus liquid only needs to be injected through the one-way water inlet valve, in the operation process, the first motor is started, the motor drives the piston to axially move along the inner part of the injection cylinder and applies certain pressure to the virus inoculation liquid in the injection cylinder, and the virus inoculation liquid is inoculated into the cell liquid under the action of the pressure; and the virus inoculation liquid is discharged through the liquid outlet nozzle, then flows into the flow guide nozzle and is finally injected into the corresponding measuring cup below, so that pollution-free and quantitative injection of the virus inoculation liquid is realized.

Description

Technical Field

[0001] This invention belongs to the field of cell culture technology, and in particular relates to a cell tolerance testing device. Background Technology

[0002] Cell culture and viral process research is one of the core areas of modern biopharmaceutical, vaccine development, and virology research. It aims to achieve efficient and controllable virus production processes by optimizing cell growth environment and virus proliferation conditions. This research field is widely used in vaccine preparation, viral vector production, antiviral drug evaluation, molecular virology research, and many other areas. In viral vaccine production, the selection of host cells and culture methods (such as adherent culture or suspension culture) have a decisive impact on viral replication efficiency and product quality. Taking mammalian cells as an example (such as Vero, BHK-21, MDCK cells, etc.), their sensitivity to infection of specific viral strains, replication support capacity, and tolerance to viral load directly determine the stability and economy of the process.

[0003] In existing technologies, cell tolerance testing typically requires the inoculation of a viral inoculum into the cell culture system to assess the cell's growth status and metabolic response under viral infection conditions. However, in practice, the viral inoculum is often added by opening the cap or through an exposed sample addition method, which easily exposes the system to the air environment. This not only increases the risk of contamination but may also affect the inoculation efficiency and the stability of the cell response due to fluctuations in gas composition, microbial interference, or changes in temperature and humidity. Consequently, it can cause uncontrollable interference with the experimental results, affecting the accuracy and repeatability of the test.

[0004] Based on this, the present invention designs a cell tolerance testing device to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to address the problem that in the prior art, cell tolerance testing usually requires the inoculation of a virus into the cell culture system to assess the cell's growth status and metabolic response under virus infection conditions. However, in actual operation, the virus inoculation solution is often added by opening the cap or by exposing the sample, which easily exposes the system to the air environment. This not only increases the risk of contamination but may also affect the inoculation efficiency and the stability of the cell response due to fluctuations in gas composition, microbial interference, or changes in temperature and humidity. Therefore, this invention proposes a cell tolerance testing device.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A cell tolerance testing device includes a housing, a switch door on one side of the housing, an injection device fixedly connected to the housing, and a receiving device and a rotating device installed inside the housing.

[0008] The injection device includes a first motor and an injection cylinder. A piston is fixedly connected to the output end of the first motor. The piston is disposed inside the injection cylinder. An outlet nozzle is fixedly connected to the bottom of the injection cylinder. A one-way water inlet valve is connected through the injection cylinder.

[0009] As a further description of the above technical solution:

[0010] The injection device also includes a fixing plate, which is fixedly connected to the upper surface of the housing, and the injection cylinder is fixedly connected to the front of the fixing plate.

[0011] As a further description of the above technical solution:

[0012] A locking plate is fixedly connected to the front of the fixing plate, and the first motor is fixedly connected to the upper surface of the locking plate.

[0013] As a further description of the above technical solution:

[0014] The receiving device includes four crossbars, which are symmetrically installed on the inner wall of the housing. A disc is fixedly connected to each of the four crossbars, and a guide nozzle is connected through the disc.

[0015] As a further description of the above technical solution:

[0016] The diameter of the cross-section of the guide nozzle is larger than the diameter of the cross-section of the outlet nozzle, and the guide nozzle and the outlet nozzle are positioned correspondingly.

[0017] As a further description of the above technical solution:

[0018] The rotating device includes four limiting rods and a mounting plate. The four limiting rods are symmetrically installed on the inner wall of the housing. One end of each limiting rod is rotatably connected to a roller, and the same turntable is mounted on the four rollers.

[0019] As a further description of the above technical solution:

[0020] The mounting plate is fixedly connected inside the housing, and a second motor is fixedly connected to the mounting plate.

[0021] As a further description of the above technical solution:

[0022] The output end of the second motor is fixedly connected to the lower surface of the turntable, and several measuring cups are installed inside the turntable.

[0023] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0024] 1. In this utility model, when it is necessary to add virus inoculation solution to cell fluid, the virus solution only needs to be injected through the one-way water inlet valve. During the operation, the first motor is started, which drives the piston to move axially along the inside of the injection cylinder and applies a certain pressure to the virus inoculation solution in the injection cylinder. Under the action of this pressure, the virus inoculation solution is discharged through the outlet, then flows into the guide nozzle, and finally injected into the corresponding measuring cup below, thereby realizing the pollution-free and quantitative injection of virus inoculation solution.

[0025] 2. In this invention, when different doses of viral inoculation solution need to be added to different measuring cups, dose difference control can be achieved by starting a second motor. The second motor drives the turntable to rotate. During the rotation, the turntable contacts and cooperates with four rollers set at its bottom to ensure stable and reliable rotation. As the turntable rotates, multiple measuring cups move synchronously in the horizontal direction. When the designated measuring cup rotates to the position directly below the guide nozzle, the quantitative injection of viral inoculation solution can be completed as needed. By controlling the rotation angle of the turntable in conjunction with the injection volume, the differential setting of the viral liquid volume in different measuring cups can be achieved, meeting the parallel needs of multiple groups of cell tolerance tests. Attached Figure Description

[0026] Figure 1 This is a three-dimensional structural diagram of a cell tolerance testing device proposed in this utility model;

[0027] Figure 2 This is a three-dimensional cross-sectional structural diagram of the housing of a cell tolerance testing device proposed in this utility model.

[0028] Figure 3 This is a three-dimensional structural diagram of the liquid injection device of the cell tolerance testing device proposed in this utility model;

[0029] Figure 4 This invention proposes a cell tolerance testing device. Figure 2 An enlarged structural diagram of part A in the middle.

[0030] Legend:

[0031] 1. Housing; 2. Opening / closing door; 3. Injection device; 301. Fixing plate; 302. Locking plate; 303. Injector; 304. Piston; 305. First motor; 306. Dispensing nozzle; 307. One-way inlet valve; 4. Receiving device; 401. Crossbar; 402. Disc; 403. Guide nozzle; 5. Rotating device; 501. Limiting rod; 502. Roller; 503. Mounting plate; 504. Second motor; 505. Turntable; 506. Measuring cup. Detailed Implementation

[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0033] Please see Figures 1-4 ,

[0034] First embodiment:

[0035] This utility model provides a technical solution: a cell tolerance testing device, including a shell 1, a switch door 2 on one side of the shell 1, an injection device 3 fixedly connected to the shell 1, and a receiving device 4 and a rotating device 5 installed inside the shell 1.

[0036] The injection device 3 includes a first motor 305 and an injection cylinder 303. The output end of the first motor 305 is fixedly connected to a piston 304, which is disposed inside the injection cylinder 303. The piston 304 is located inside the injection cylinder 303, and the two work together to form a controllable closed cavity. Driven by the first motor 305, the piston 304 can reciprocate inside the injection cylinder 303, thereby applying a stable thrust to the virus inoculation solution, realizing a uniform and controllable pressurized injection process, and avoiding the impact of injection fluctuations on the test results.

[0037] A dispensing nozzle 306 is fixedly connected to the bottom of the syringe 303, and a one-way water inlet valve 307 is connected through the syringe 303.

[0038] Specifically, such as Figure 2-3 As shown, the injection device 3 also includes a fixing plate 301, which is fixedly connected to the upper surface of the housing 1. The injection cylinder 303 is fixedly connected to the front of the fixing plate 301. A locking plate 302 is fixedly connected to the front of the fixing plate 301. The first motor 305 is fixedly connected to the upper surface of the locking plate 302. The receiving device 4 includes four crossbars 401, which are symmetrically installed on the inner wall of the housing 1. A disc 402 is fixedly connected to the four crossbars 401. The four crossbars 401 are symmetrically distributed on the inner wall of the housing 1 to stably support the disc 402 and ensure that it remains flat in the horizontal plane. This mating structure improves the accuracy of the guide nozzle 403 in aligning with the measuring cup 506 below, avoids injection deviation, and enhances the load-bearing stability of the disc 402.

[0039] A guide nozzle 403 is connected through the disc 402. The diameter of the cross-section of the guide nozzle 403 is larger than the diameter of the cross-section of the outlet nozzle 306. The guide nozzle 403 and the outlet nozzle 306 are positioned correspondingly. The outlet nozzle 306 is located below the syringe 303 and directly opposite the guide nozzle 403 on the disc 402. The cross-section of the guide nozzle 403 is larger than that of the outlet nozzle 306, which can effectively receive and guide the fluid to flow steadily into the measuring cup 506. The precise correspondence between the two positions avoids liquid dripping or splashing, improving the accuracy of liquid injection and the cleanliness of the device.

[0040] During operation, when it is necessary to add virus inoculation solution to the cell fluid, the virus solution is simply injected through the one-way water inlet valve 307. During the operation, the first motor 305 is started, which drives the piston 304 to move axially along the inside of the syringe 303 and applies a certain pressure to the virus inoculation solution in the syringe 303. Under this pressure, the virus inoculation solution is discharged through the outlet 306, then flows into the guide nozzle 403, and finally is injected into the corresponding measuring cup 506 below, thereby achieving contamination-free and quantitative injection of virus inoculation solution.

[0041] Second embodiment:

[0042] Specifically, such as Figure 4 As shown, the rotating device 5 includes four limiting rods 501 and a mounting plate 503. The four limiting rods 501 are symmetrically installed on the inner wall of the housing 1. One end of each limiting rod 501 is rotatably connected to a roller 502. The same turntable 505 is mounted on the four rollers 502. The mounting plate 503 is fixedly connected inside the housing 1. A second motor 504 is fixedly connected to the mounting plate 503. The output end of the second motor 504 is fixedly connected to the lower surface of the turntable 505. Several measuring cups 506 are installed inside the turntable 505.

[0043] During operation, when different doses of viral inoculation solution need to be added to different measuring cups 506, dose difference control can be achieved by activating the second motor 504. The second motor 504 drives the turntable 505 to rotate. During rotation, the turntable 505 contacts and engages with the four rollers 502 located at its bottom to ensure stable and reliable rotation. As the turntable 505 rotates, multiple measuring cups 506 move synchronously in the horizontal direction. When the designated measuring cup 506 rotates to the position directly below the guide nozzle 403, the quantitative injection of viral inoculation solution can be completed as needed. By controlling the rotation angle of the turntable 505 in conjunction with the injection volume, the differential setting of the viral solution volume in different measuring cups 506 can be achieved, meeting the parallel needs of multiple groups of cell tolerance tests.

[0044] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A cell tolerance testing device, comprising a housing (1), characterized in that, A switch door (2) is provided on one side of the housing (1), a liquid injection device (3) is fixedly connected to the housing (1), and a receiving device (4) and a rotating device (5) are installed inside the housing (1). The injection device (3) includes a first motor (305) and an injection cylinder (303). A piston (304) is fixedly connected to the output end of the first motor (305). The piston (304) is disposed inside the injection cylinder (303). An outlet nozzle (306) is fixedly connected to the bottom of the injection cylinder (303). A one-way water inlet valve (307) is connected through the injection cylinder (303).

2. The cell tolerance testing device according to claim 1, characterized in that, The injection device (3) also includes a fixing plate (301), which is fixedly connected to the upper surface of the housing (1), and the injection cylinder (303) is fixedly connected to the front of the fixing plate (301).

3. The cell tolerance testing device according to claim 2, characterized in that, A locking plate (302) is fixedly connected to the front of the fixing plate (301), and the first motor (305) is fixedly connected to the upper surface of the locking plate (302).

4. The cell tolerance testing device according to claim 1, characterized in that, The receiving device (4) includes four crossbars (401), which are symmetrically installed on the inner wall of the housing (1). A disc (402) is fixedly connected to the four crossbars (401), and a guide nozzle (403) is connected through the disc (402).

5. The cell tolerance testing device according to claim 4, characterized in that, The diameter of the cross-section of the guide nozzle (403) is larger than the diameter of the cross-section of the liquid outlet nozzle (306), and the guide nozzle (403) and the liquid outlet nozzle (306) are positioned opposite each other.

6. The cell tolerance testing device according to claim 1, characterized in that, The rotating device (5) includes four limiting rods (501) and a mounting plate (503). The four limiting rods (501) are symmetrically installed on the inner wall of the housing (1). One end of each limiting rod (501) is rotatably connected to a roller (502), and the same turntable (505) is attached to each of the four rollers (502).

7. The cell tolerance testing device according to claim 6, characterized in that, The mounting plate (503) is fixedly connected inside the housing (1), and a second motor (504) is fixedly connected to the mounting plate (503).

8. The cell tolerance testing device according to claim 7, characterized in that, The output end of the second motor (504) is fixedly connected to the lower surface of the turntable (505), and several measuring cups (506) are installed inside the turntable (505).

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