Device for detecting repeated sample suction precision of pipettor

Abstract

The device comprises a horizontal moving structure, a vertical cantilever structure, a telescopic arm structure and a control part, a moving seat horizontally moving along the horizontal moving structure is arranged on the upper portion of the horizontal moving structure, one end of the vertical cantilever structure is fixedly connected with the moving seat, and the other end of the vertical cantilever structure is fixedly connected with the telescopic arm structure. The other end of the vertical cantilever structure is movably connected with the upper portion of the telescopic arm structure, the vertical cantilever structure and the horizontal moving structure are perpendicularly crossed through a moving seat, and a connecting part is arranged on the lower portion of the telescopic arm structure, detachably connected with a pipettor and capable of moving up and down in a telescopic mode along the telescopic arm structure. The control part is connected with the horizontal moving structure, the moving seat, the vertical cantilever structure, the telescopic arm structure and the connecting part in a signal control manner; and the control part can perform signal control on the horizontal moving structure, the moving seat, the vertical cantilever structure, the telescopic arm structure and the connecting part. The device can efficiently detect whether the sample suction precision of the pipettor meets the precision requirement or not, and has the advantages of simple structure and simplicity and convenience in operation.

Description

Technical Field

[0001] This utility model relates to the field of fluorescence immunoassay quantitative analysis technology, and in particular to a device for detecting the accuracy of repeated pipette sampling. Background Technology

[0002] Fluorescence immunoassay combines the high sensitivity of fluorescence technology with the high specificity of immunological techniques, providing a unique and irreplaceable detection method for immunology, clinical histochemistry, and laboratory diagnostics. Automated fluorescence immunoassay analyzers, as the application platform for fluorescence immunoassay, are widely used not only for the identification of bacteria, viruses, protozoa, worms, and fungi, and the diagnosis of related diseases, but also for the detection of serum antibodies (including autoantibodies), the diagnosis and research of autoimmune diseases, and the diagnosis and research of tumor immunology.

[0003] In operation, the automated fluorescence immunoassay analyzer uses a pipette to transfer a certain amount of sample to the reagent card, then drops the quantitative sample onto the reagent card for quantitative analysis and detection.

[0004] The accuracy of pipette aspiration is directly related to the accuracy of quantitative analysis by the analyzer. Especially after initial use and subsequent use, it is necessary to test the pipette aspiration accuracy. This involves confirming that the volume aspirated by the pipette is consistent each time and that the pipette is consistently inserted below the liquid surface to ensure the accuracy of the analyzer's quantitative analysis. Therefore, in practical use of the analyzer, it is frequently necessary to quickly test the pipette aspiration accuracy to guarantee the accuracy of the analyzer's quantitative analysis.

[0005] This application discloses a device for detecting the repeatability of pipette sampling, which aims to efficiently detect whether the pipette sampling accuracy meets the accuracy requirements and ensure the accuracy of quantitative analysis by the analyzer. Utility Model Content

[0006] To address the problems in related technologies, this application discloses a device for detecting the repeatability of pipette sampling, which can efficiently detect whether the pipette sampling accuracy meets the accuracy requirements.

[0007] To achieve the above objectives, this application provides the following technical solution:

[0008] A device for detecting the repeatability of pipette sampling includes a horizontal moving structure, a vertical cantilever structure, a telescopic arm structure, and a control unit. The upper part of the horizontal moving structure has a movable seat that moves horizontally along the structure. One end of the vertical cantilever structure is fixedly connected to the movable seat, and the other end is movably connected to the upper part of the telescopic arm structure. The vertical cantilever structure and the horizontal moving structure intersect perpendicularly through the movable seat. The lower part of the telescopic arm structure has a connecting part that detachably connects to the pipette and can extend and retract along the telescopic arm structure. The control unit is signal-controlled connected to the horizontal moving structure, the movable seat, the vertical cantilever structure, the telescopic arm structure, and the connecting part, and can perform signal control on these components.

[0009] The working principle of this application is as follows:

[0010] Parameter setting stage: First, the control unit moves the horizontal moving structure and the vertical cantilever structure to move the pipette to the detection point and the measurement point. A detection measuring cup containing a certain amount of liquid is placed at the detection point, and an empty measurement measuring cup is placed at the measurement point. The controller determines the positioning coordinates of the detection point and the measurement point and sets the number of repetitions of the detection. After the parameter setting is completed, automatic detection begins.

[0011] Automatic detection phase: After the parameters are set, automatic detection begins. The pipette moves repeatedly between the detection point and the measurement point, automatically completing the aspiration and dispensing process according to the preset number of detection repetitions until the detection is completed.

[0012] Testing Process: During the automatic testing phase, the testing personnel observe whether the depth of the pipette tip penetrating the liquid surface each time during liquid aspiration meets the requirements. By evaluating a certain number of liquid aspiration processes, it is possible to quickly determine whether the depth of the pipette penetrating the liquid surface each time is qualified, thus solving the problem of difficulty in quantitatively analyzing the depth of each pipette penetration. After the automatic testing is completed, the average liquid volume per aspiration is quickly estimated by measuring the volume of liquid collected in the measuring cup placed at the measuring point, thus solving the problem of difficulty in rapid quantitative evaluation due to the small amount of liquid aspirated per pipette.

[0013] As a further embodiment of this application, the horizontally moving structure includes a horizontally moving frame, a horizontally moving track, and a horizontally moving power device. The horizontally moving power device includes a horizontally moving drive motor and a horizontally moving belt structure. The moving seat is slidably connected to the horizontally moving track, and a fixing plate is provided at the bottom of the moving seat, which is fixedly connected to the horizontally moving belt structure. When the horizontally moving power device drives the horizontally moving belt mechanism to move, the fixing plate moves together with the horizontally moving belt structure, causing the moving seat to slide along the horizontally moving track.

[0014] As a further embodiment of this application, the movable base includes an upper movable connecting plate and a lower movable slot. The movable slot is matched with a horizontal moving track, and the movable connecting plate is fixedly connected to a vertical cantilever structure. When the horizontal moving power device drives the fixed plate at the bottom of the movable base to move, the movable slot at the lower end of the movable base slides along the horizontal moving track, causing the movable base, the upper movable connecting plate, and the vertical cantilever structure to slide together along the horizontal moving track.

[0015] As a further embodiment of this application, two horizontal moving tracks and moving slots are provided, respectively located on both sides of the lower end of the moving seat. This embodiment is beneficial for the horizontal moving power device to drive the vertical cantilever structure to slide along the horizontal moving track more smoothly.

[0016] As a further embodiment of this application, the vertical cantilever structure includes a cantilever frame, a cantilever moving track, and a cantilever moving power device. The cantilever moving power device includes a cantilever moving drive motor and a cantilever moving belt structure, and the cantilever moving track is slidably connected to the telescopic arm structure.

[0017] As a further embodiment of this application, the telescopic arm structure includes a telescopic frame, with a telescopic moving slot and a telescopic fixing plate at the top of the frame. The telescopic moving slot is matched with the cantilever moving track, and the telescopic fixing plate is fixedly connected to the cantilever moving belt structure. It also includes a telescopic power device, with a connecting part at its moving end that can be connected to a pipette. The telescopic power device is a lead screw motor structure. When the cantilever moving drive motor drives the cantilever moving belt structure to move, the telescopic fixing plate moves together with the cantilever moving belt structure, causing the telescopic arm structure to slide along the cantilever moving track.

[0018] As a further embodiment of this application, two cantilever moving tracks and telescopic moving slots are provided, arranged in parallel on the cantilever frame. This embodiment is beneficial for the telescopic arm structure to run more smoothly when sliding along the cantilever moving track.

[0019] As a further embodiment of this application, the connecting part includes a connecting plate with multiple connecting holes, and connecting screws are used to detachably connect to the pipette through the connecting holes.

[0020] By adopting the above technical solution, the position coordinates of the detection point and the measurement point can be quickly obtained by using a two-dimensional plane coordinate system composed of a horizontal moving structure and a vertical cantilever structure; the visual inspection method can be used to quantitatively detect the depth of the pipette into the liquid surface each time and the volume of liquid aspirated each time. It has the advantages of simple structure, convenient operation and easy implementation.

[0021] In summary, the beneficial effects of this application are as follows:

[0022] 1. Utilizing a two-dimensional plane coordinate system composed of a horizontal moving structure and a vertical cantilever structure, the placement position of the measuring cup and the detection cup can be quickly located. The coordinates can be easily adjusted according to the operating environment. There is no need to pre-fix the position, and it can be used immediately after placement. The telescopic arm structure enables the pipette to move vertically, realizing the downward and upward operation of the pipette needle during the aspiration and dispensing process. The entire aspiration and dispensing process is automated and easy to operate.

[0023] 2. The method of visual inspection is used to directly determine whether the liquid level of the pipette is qualified each time, and the method of calculating the average liquid volume is used to quickly assess whether the liquid volume drawn by the pipette each time is qualified. The detection process is simple and fast. Attached Figure Description

[0024] The accompanying drawings are provided to further understand this application and form part of the specification. They are used together with the embodiments of this application to explain this application and do not constitute a limitation thereof.

[0025] In the attached diagram:

[0026] Figure 1 This is a schematic diagram of a device for detecting the repeatability of pipette sampling according to this application.

[0027] Figure 2 This is a top view of the horizontal moving structure of this application.

[0028] Figure 3 This is a bottom view of the horizontal moving structure of this application.

[0029] Figure 4 This is a structural diagram of the vertical cantilever structure and its adjacent structures in this application.

[0030] Figure 5 This is a schematic diagram of the telescopic arm structure of this application.

[0031] Figure 6 This is a schematic diagram of the device for detecting the repeatability of pipette sampling accuracy according to this application, in its working state.

[0032] Figure 7 This is a schematic diagram of the control signal connection of a device for detecting the repeatability of pipette sampling according to this application.

[0033] Figure label annotations:

[0034] 1. Horizontal moving structure; 11. Horizontal moving frame; 12. Horizontal moving track; 13. Horizontal moving power unit; 14. Horizontal moving drive motor; 15. Horizontal moving belt structure; 2. Moving seat; 21. Fixing plate; 22. Moving slot; 23. Moving slot; 3. Vertical cantilever structure; 31. Cantilever frame; 32. Cantilever moving track; 33. Cantilever moving power unit; 34. Cantilever moving drive motor; 35. Cantilever moving belt structure; 4. Telescopic arm structure; 41. Telescopic frame; 42. Telescopic moving slot; 43. Telescopic power unit; 44. Telescopic fixing plate; 5. Connecting part; 51. Connecting plate; 52. Connecting hole; 6. Control part. Detailed Implementation

[0035] Exemplary embodiments will now be described in detail, examples of which are illustrated in the accompanying drawings. When the following description relates to the drawings, unless otherwise indicated, the same numbers in different drawings denote the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with this application. Rather, they are merely examples of apparatuses and methods consistent with some aspects disclosed in this embodiment as detailed in the appended claims.

[0036] It should be noted that all directional indicators in the embodiments (such as up, down, left, right, front, back, etc.) are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicator will also change accordingly.

[0037] Furthermore, the use of terms such as "first" and "second" in the embodiments is for descriptive purposes only and does not specifically refer to any order or sequence, nor is it intended to limit this application. It is merely to distinguish components or operations described using the same technical terms and should not be construed as indicating or implying their relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions of the various embodiments can be combined with each other, but only if they are feasible for those skilled in the art. If a combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed in this application.

[0038] To further understand the content, features, and effects of this application, the following embodiments are provided, and detailed descriptions are given below in conjunction with the accompanying drawings: Specific Implementation

[0039] like Figure 1 , Figure 7As shown, a device for detecting the repeatability of pipette sampling includes a horizontal moving structure 1, a vertical cantilever structure 3, a telescopic arm structure 4, and a control unit 6. The horizontal moving structure 1 has a movable seat 2 that moves horizontally along it. One end of the vertical cantilever structure 3 is fixedly connected to the movable seat 2, and the other end is movably connected to the upper part of the telescopic arm structure 4. The vertical cantilever structure 3 and the horizontal moving structure 1 intersect perpendicularly through the movable seat 2. The lower part of the telescopic arm structure 4 has a connecting part 5, which is detachably connected to the pipette. The connecting part 5 can extend and retract along the telescopic arm structure 4. The control unit 6 is signal-controlled connected to the horizontal moving structure 1, the movable seat 2, the vertical cantilever structure 3, the telescopic arm structure 4, and the connecting part 5. The control unit 6 can perform signal control on the horizontal moving structure 1, the movable seat 2, the vertical cantilever structure 3, the telescopic arm structure 4, and the connecting part 5.

[0040] like Figure 2 , Figure 3 As shown, the horizontal moving structure 1 includes a horizontal moving frame 11, a horizontal moving track 12, and a horizontal moving power device 13. The horizontal moving power device 13 includes a horizontal moving drive motor 14 and a horizontal moving belt structure 15. The moving seat 2 is slidably connected to the horizontal moving track 12. A fixing plate 21 is provided at the bottom of the moving seat 2, and the fixing plate 21 is fixedly connected to the horizontal moving belt structure 15.

[0041] like Figure 1 , Figure 2 , Figure 3 As shown, the movable seat 2 includes a movable connecting plate 22 at the upper end and a movable slot 23 at the lower end. The movable slot 23 is matched with the horizontal moving track 12, and the movable connecting plate 22 is fixedly connected to the vertical cantilever structure 3.

[0042] like Figure 2 As shown, the horizontal moving track 12 and the moving slot 23 are provided in two ways.

[0043] like Figure 4 As shown, the vertical cantilever structure 3 includes a cantilever frame 31, a cantilever moving track 32, and a cantilever moving power device 33. The cantilever moving power device 33 includes a cantilever moving drive motor 34 and a cantilever moving belt structure 35. The cantilever moving track 32 is slidably connected to the telescopic arm structure 4.

[0044] like Figure 4 , Figure 5 As shown, the telescopic arm structure 4 includes a telescopic frame 41, with a telescopic moving slot 42 and a telescopic fixing plate 44 at the top of the telescopic frame 41. The telescopic moving slot 42 is matched with the cantilever moving track 32, and the telescopic fixing plate 44 is fixedly connected to the cantilever moving belt structure 35. It also includes a telescopic power device 43, with a connecting part 5 at the moving end of the telescopic power device 43. The connecting part 5 can be connected to a pipette. The telescopic power device 43 is a screw motor structure.

[0045] like Figure 4 As shown, the cantilever moving track 32 and the telescopic moving slot 42 are provided in two ways.

[0046] like Figure 5 As shown, the connecting part 5 includes a connecting plate 51, which has a plurality of connecting holes 52. The connecting part 51 is detachably connected to the pipette through the connecting holes 52 by connecting screws.

[0047] The implementation principle of the device for detecting the repeatability of pipette sampling according to the embodiments of this application is as follows:

[0048] like Figure 6 , Figure 7 As shown, before starting the test, a measuring cup containing a certain amount of liquid is placed at test point A, and an empty measuring cup is placed at measurement point B. The number of repetitions N is set. The horizontal moving structure 1 of the control unit 6 moves horizontally along the X-axis, and the vertical cantilever structure 3 moves horizontally along the Y-axis, so that the pipette is moved to test point A and the XY-axis coordinate values ​​of test point A are recorded. Then, the pipette is moved to measurement point B and the telescopic arm structure 4 is lowered, so that the bottom of the pipette needle is inserted into a suitable position in the measuring cup. This suitable position should be above the liquid surface of the maximum liquid volume of the measuring cup in this test. The XY-axis coordinate values ​​of measurement point B and the depth of the pipette needle inserted into the measuring cup are recorded. After the parameter settings are completed, the automatic test begins.

[0049] After the parameters are set, automatic detection begins. The control unit 6 controls the pipette needle to enter the detection cup at detection point A, aspirate the liquid, move it to measurement point B, and drip the liquid into the measurement cup. The detection is repeated N times according to the preset settings, and the liquid aspiration and dripping processes are completed automatically until the detection ends.

[0050] During the automatic detection phase, when the pipette is in the liquid aspiration state, the tester can directly observe and quickly determine whether the depth of the pipette tip penetrating the liquid surface in the test volumetric cup each time meets the requirements based on the scale on the test volumetric cup. By evaluating a certain number of liquid aspiration processes, it is possible to quickly determine whether the depth of the pipette penetrating the liquid surface each time is qualified, thus solving the problem of difficulty in quantitatively analyzing the depth of each pipette penetration. After the automatic detection is completed, by measuring the volume of liquid collected in the measuring cup placed at the measurement point, the average volume of liquid aspiration per time can be quickly estimated, solving the problem of difficulty in rapid quantitative evaluation due to the small volume of liquid aspirated by the pipette each time.

[0051] For example, if the required depth of the pipette needle to be inserted is 1 mm below the liquid surface, and the volume of liquid aspirated each time is 50 μL, then during the parameter setting stage, the number of repetitions of the test can be set to 20. The coordinates of test point A and measurement point B are determined based on the positions of the test and measurement cups. The telescopic arm structure 4 is then controlled to insert the pipette needle to a suitable position in the measurement cup where the depth is above 1000 μL, ensuring that the pipette needle remains above the liquid surface in the measurement cup when the automatic test is completed. After the automatic test begins, the pipette aspirates liquid from point A and then moves to point B to dispense liquid, repeating this operation 20 times. The depth of the pipette needle is observed online to ensure it remains 1 mm below the liquid surface and is recorded. At the end of the test, the cumulative liquid volume in the measurement cup is observed to ensure it reaches 1000 μL, thus determining whether the pipette's sampling accuracy meets the requirements.

[0052] Finally, it should be noted that the above disclosure is merely a preferred embodiment of this application and is not intended to limit this application. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application. The scope of this application is limited only by the appended claims.

Claims

1. A device for detecting the accuracy of repeated pipette sampling, characterized in that: The system includes a horizontal moving structure (1), a vertical cantilever structure (3), a telescopic arm structure (4), and a control unit (6). The horizontal moving structure (1) has a movable seat (2) on its upper part that moves horizontally along it. One end of the vertical cantilever structure (3) is fixedly connected to the movable seat (2), and the other end is movably connected to the upper part of the telescopic arm structure (4). The vertical cantilever structure (3) and the horizontal moving structure (1) intersect perpendicularly through the movable seat (2). The telescopic arm structure... (4) A connecting part (5) is provided at the bottom. The connecting part (5) is detachably connected to the pipette. The connecting part (5) can move up and down along the telescopic arm structure (4). The control part (6) can be connected to the horizontal moving structure (1), the moving seat (2), the vertical cantilever structure (3), the telescopic arm structure (4), and the connecting part (5) by signal control. The control part (6) can perform signal control on the horizontal moving structure (1), the moving seat (2), the vertical cantilever structure (3), the telescopic arm structure (4), and the connecting part (5).

2. The device for detecting the repeatability of pipette sampling according to claim 1, characterized in that: The horizontal moving structure (1) includes a horizontal moving frame (11), a horizontal moving track (12), and a horizontal moving power device (13). The horizontal moving power device (13) includes a horizontal moving drive motor (14) and a horizontal moving belt structure (15). The moving seat (2) is slidably connected to the horizontal moving track (12). A fixing plate (21) is provided at the bottom of the moving seat (2). The fixing plate (21) is fixedly connected to the horizontal moving belt structure (15).

3. The device for detecting the repeatability of pipette sampling according to claim 2, characterized in that: The movable seat (2) includes a movable connecting plate (22) at the upper end and a movable slot (23) at the lower end. The movable slot (23) is matched with the horizontal moving track (12), and the movable connecting plate (22) is fixedly connected to the vertical cantilever structure (3).

4. The device for detecting the repeatability of pipette sampling according to claim 3, characterized in that: The horizontal moving track (12) and the moving slot are provided in two sets.

5. The device for detecting the repeatability of pipette sampling according to claim 1, characterized in that: The vertical cantilever structure (3) includes a cantilever frame (31), a cantilever moving track (32), and a cantilever moving power device (33). The cantilever moving power device (33) includes a cantilever moving drive motor (34) and a cantilever moving belt structure (35). The cantilever moving track (32) is slidably connected to the telescopic arm structure (4).

6. The apparatus for detecting the repeatability of pipette sampling according to claim 5, characterized in that: The telescopic arm structure (4) includes a telescopic frame (41), the top of which is provided with a telescopic moving slot (42) and a telescopic fixing plate (44). The telescopic moving slot (42) is matched with the cantilever moving track (32), and the telescopic fixing plate (44) is fixedly connected to the cantilever moving belt structure (35). It also includes a telescopic power device (43), the moving end of which is provided with the connecting part (5). The connecting part (5) can be connected to a pipette. The telescopic power device (43) is a screw motor structure.

7. The device for detecting the repeatability of pipette sampling according to claim 6, characterized in that: The cantilever moving track (32) and the telescopic moving slot (42) are provided in two ways.

8. The device for detecting the repeatability of pipette sampling according to claim 1, characterized in that: The connecting part (5) includes a connecting plate (51), which has a plurality of connecting holes (52) and is detachably connected to the pipette by means of connecting screws through the connecting holes (52).

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