In-vitro culture equipment for lung cancer tissues

Abstract

The utility model provides lung cancer tissue in-vitro culture equipment which comprises a culture box, one side of the culture box is fixedly connected with an observation window, and the bottom of the culture box is fixedly connected with a bottom support frame; according to the utility model, the heights of the four supporting blocks are respectively adjusted, so that the inclination of the incubator can be adjusted, the horizontality of the incubator on different inclined placing surfaces is kept, and the influence of inclination on the activity of cells is reduced; a disinfectant flowing out of the side liquid spraying pipe is sprayed out in an atomized mode through the atomizing nozzle, disinfection work is conducted on the interior of the incubator, the workload of operators is reduced, the incubator does not need to be opened, and the situation that external pollutants are brought in during manual disinfection is reduced; meanwhile, a rotating connecting rod can drive a transmission fan to rotate along the inner side of a top mounting hole, gas in the incubator is pumped out, volatilization of a disinfectant in the incubator is accelerated, residues are reduced, the disinfection effect in the incubator is improved, and the stability after a sealing plate and a metal side plate are connected is improved through magnetic attraction of a side mounting hole and a magnetic attraction plate.

Description

Technical Field

[0001] This utility model relates to the field of cell culture, and in particular to an in vitro culture device for lung cancer tissue. Background Technology

[0002] Lung cancer tissue is a malignant tumor originating from the epithelium of the bronchi, bronchioles, or alveoli. In vitro culture equipment provides a controllable experimental platform for lung cancer research by simulating the human microenvironment. By using patient tumor cells to construct "surrogates," the genetic heterogeneity and microenvironment interaction of tumors can be replicated. In vitro models reduce the need for animal experiments, shorten the research and development cycle, and reduce the cost of new drug development.

[0003] In the prior art, patent application document "CN218115463U" discloses an "in vitro culture device for lung cancer tissue"; including a culture dish, a base at the lower end of the culture dish, a support and clamping structure at the lower end of the base, and a positioning structure at the upper end of the base; a cap, which is placed on the outer side of the upper end of the culture dish and positioned by the positioning structure, and a sealing structure at the upper end of the cap, with a handle fixedly connected to the middle of the upper end of the cap. The beneficial effects are: this utility model can increase the sealing effect of the cap by setting the sealing structure and the fastening structure, and can fasten the cap after it is put on, so as to avoid the cap shaking and gaps affecting the sealing effect. It also has a detachable support and clamping structure, which can clamp and fasten the cell culture membrane to the bottom of the culture dish, preventing the cell culture membrane from wrinkling during the experimental operation and affecting the culture effect. Moreover, it can facilitate the disassembly and replacement of the cell culture membrane, increasing the convenience of use.

[0004] The aforementioned “in vitro culture device for lung cancer tissue” still has some drawbacks. For example, existing in vitro cell culture devices need to be placed horizontally during use. If the device is tilted during placement, liquid will accumulate on one side, causing cells to be exposed to air or have excessive or insufficient nutrients, affecting proliferation and activity. Furthermore, after completing the in vitro culture experiment on lung cancer tissue, the chamber needs to be manually disinfected, which may introduce the risk of bacteria.

[0005] To address these issues, an in vitro culture device for lung cancer tissue is proposed here. Utility Model Content

[0006] The technical problem to be solved by this utility model is to overcome the defects of the existing technology. This utility model proposes an in vitro culture device for lung cancer tissue.

[0007] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is: an in vitro culture device for lung cancer tissue, including a culture chamber, an observation window fixedly connected to one side of the culture chamber, a bottom support frame fixedly connected to the bottom of the culture chamber, side lifting frames opened on both sides of the bottom support frame, a bottom bearing plate movably connected to the bottom of the culture chamber, a gripping plate movably connected to the bottom of the bottom bearing plate, a threaded column fixedly connected to the bottom of the gripping plate, support blocks provided on both sides of the bottom support frame, and threaded holes opened on the surface of the support blocks.

[0008] Preferably, the side lifting frame has side slide rails on both sides, and a sliding block is fixedly connected to one side of the support block, with the surface of the sliding block slidably connected to one side of the side slide rail.

[0009] Preferably: an inner connecting frame is provided on the inner side of the bottom support frame, and a side connecting groove is provided on the other side of the bottom support frame. A liquid storage tank is fixedly connected to the inner side of the inner connecting frame. An inlet pipe is fixedly connected to one side of the liquid storage tank. A bottom water pump is fixedly connected to the other side of the liquid storage tank. An outlet pipe is provided on one side of the bottom water pump. A top water tank is fixedly connected to one end of the outlet pipe. A top water pump is fixedly connected to one side of the top water tank. A side spray pipe is provided on one side of the top water pump. An atomizing nozzle is fixedly connected to one end of the side spray pipe. A top connecting hole is provided on the top of the incubator. A heat sink is fixedly connected to one side of the top water tank.

[0010] Preferably, an inner fixing plate is fixedly connected to the inner side of the top water tank, a top bearing plate is movably connected to one side of the inner fixing plate, a transmission plate is movably connected to one side of the top bearing plate, a transmission rod is fixedly connected to one side of the transmission plate, and an agitator is fixedly connected to one end of the transmission rod.

[0011] Preferably, the incubator has a top protective cylinder on its top, a top mounting hole is fixedly connected to the inner side of the top protective cylinder, a top fixing frame is fixedly connected to the top of the top mounting hole, a motor body is fixedly connected to one side of the top fixing frame, a connecting rod is fixedly connected to the output shaft of the motor body, and a transmission fan is fixedly connected to one end of the connecting rod.

[0012] Preferably, a metal side plate is fixedly connected to the top of the top mounting hole, a side mounting hole is opened on one side of the metal side plate, a rubber ring is fixedly connected to the top of the metal side plate, a sealing plate is provided on one side of the metal side plate, a friction block is fixedly connected to the top of the sealing plate, a magnetic suction plate is fixedly connected to one side of the sealing plate, and an arc-shaped groove is opened on one side of the sealing plate and one side of the metal side plate.

[0013] Preferably, a side bearing disk is movably connected to one side of the transmission fan, a bottom fixing frame is movably connected to one side of the side bearing disk, and the two sides of the bottom fixing frame are fixedly connected to the inner side of the top mounting hole.

[0014] Compared with the prior art, the beneficial effects of this utility model include:

[0015] 1. Utilizing the threaded connection between the threaded column and the threaded hole, the rotation of the threaded column will drive the support block to move vertically up and down along the inner side of the side lifting frame. By adjusting the height of the four support blocks respectively, the inclination of the incubator can be adjusted to maintain the horizontality of the incubator on different inclined placement surfaces, reducing the impact of tilt on cell activity.

[0016] 2. By utilizing the opening of the top connecting hole, one end of the side spray pipe is fixed inside the incubator. At this time, the disinfectant flowing out of the side spray pipe is sprayed out in atomized form through the atomizing nozzle to disinfect the incubator. This reduces the workload of the operators and eliminates the need to open the incubator, thus reducing the possibility of introducing external contaminants during manual disinfection.

[0017] 3. When the disinfectant enters the top water tank, it impacts the transmission plate. The fan-shaped transmission plate with an inclined angle rotates along the top bearing plate after being impacted by the liquid. The rotating transmission plate drives the transmission rod to rotate, and the rotating transmission rod drives the agitator to agitate the disinfectant in the top water tank, which improves the activity of the disinfectant, reduces sedimentation, and improves the disinfection effect when the disinfectant is sprayed out along the atomizing nozzle.

[0018] 4. The motor body drives the connecting rod to rotate. The rotating connecting rod will drive the transmission fan to rotate along the inner side of the top mounting hole. At the same time, when the transmission fan rotates, it will be movably supported on one side of the bottom fixed frame through the side bearing plate, which improves the stability of the transmission fan rotation, and extracts the gas in the incubator, accelerates the evaporation of the disinfectant in the incubator, reduces residue, and improves the disinfection effect in the incubator. In addition, the magnetic attraction between the side mounting hole and the magnetic suction plate improves the stability of the connection between the sealing plate and the metal side plate. Attached Figure Description

[0019] The disclosure of this utility model is illustrated with reference to the accompanying drawings. It should be understood that the drawings are for illustrative purposes only and are not intended to limit the scope of protection of this utility model. In the drawings, the same reference numerals are used to refer to the same parts. Wherein:

[0020] Figure 1 The schematic diagram shows a structural schematic of an observation window according to one embodiment of the present invention;

[0021] Figure 2 The schematic diagram shows a top protective cylinder structure according to one embodiment of the present invention;

[0022] Figure 3 The schematic diagram shows a structural schematic of a support block according to one embodiment of the present invention;

[0023] Figure 4 The schematic diagram shows a structural schematic of an outlet pipe according to one embodiment of the present invention;

[0024] Figure 5 The schematic diagram shows a structural schematic of a top water tank according to one embodiment of the present invention;

[0025] Figure 6 The schematic diagram shows a structural schematic of a side bearing disc according to one embodiment of the present invention.

[0026] Numbering on the map:

[0027] 1. Incubator; 2. Observation window; 301. Bottom support frame; 302. Side lifting frame; 303. Bottom bearing plate; 304. Holding plate; 305. Threaded column; 306. Support block; 307. Threaded hole; 308. Sliding block; 309. Side slide rail; 401. Inner connecting frame; 402. Liquid storage tank; 403. Side connecting groove; 404. Liquid inlet pipe; 405. Bottom water pump; 406. Liquid outlet pipe; 407. Top water tank; 408. Heat sink; 409. Top water pump; 4010. Side spray pipe; 4011. Top connecting hole; 40 12. Atomizing nozzle; 501. Inner fixing plate; 502. Top bearing plate; 503. Transmission plate; 504. Transmission rod; 505. Stirring frame; 601. Top protective cylinder; 602. Top mounting hole; 603. Top fixing frame; 604. Motor body; 605. Connecting rod; 606. Transmission fan; 607. Side bearing plate; 608. Bottom fixing frame; 609. Metal side plate; 6010. Rubber ring; 6011. Side mounting hole; 6012. Sealing plate; 6013. Friction block; 6014. Magnetic suction plate; 6015. Arc groove. Detailed Implementation

[0028] It is readily understood that, based on the technical solution of this utility model, those skilled in the art can propose various interchangeable structural methods and implementations without altering the essential spirit of this utility model. Therefore, the following detailed embodiments and accompanying drawings are merely illustrative descriptions of the technical solution of this utility model and should not be considered as the entirety of this utility model or as limitations or restrictions on the technical solution of this utility model.

[0029] According to one embodiment of the present invention, in conjunction with Figures 1 to 6The diagram shows an in vitro culture device for lung cancer tissue, including an incubator 1. An observation window 2 is fixedly connected to one side of the incubator 1. A bottom support frame 301 is fixedly connected to the bottom of the incubator 1. Side lifting frames 302 are provided on both sides of the bottom support frame 301. A bottom bearing plate 303 is movably connected to the bottom of the incubator 1. A gripping plate 304 is movably connected to the bottom of the bottom bearing plate 303. A threaded column 305 is fixedly connected to the bottom of the gripping plate 304. Support blocks 306 are provided on both sides of the bottom support frame 301. Threaded holes 307 are provided on the surface of the support blocks 306.

[0030] It should be noted that by utilizing the threaded connection between the threaded post 305 and the threaded hole 307, the rotation of the threaded post 305 will drive the support block 306 to move vertically up and down along the inner side of the side lifting frame 302.

[0031] In this embodiment, side slide rails 309 are provided on both sides of the side lifting frame 302, and a sliding block 308 is fixedly connected to one side of the support block 306. The surface of the sliding block 308 is slidably connected to one side of the side slide rail 309.

[0032] It should be noted that when the support block 306 moves vertically, it will cause the sliding block 308 to slide along the inner side of the side slide rail 309, which improves the stability of the support block 306 when it moves vertically.

[0033] In this embodiment, an inner connecting frame 401 is provided on the inner side of the bottom support frame 301, and a side connecting groove 403 is provided on the other side of the bottom support frame 301. A liquid storage tank 402 is fixedly connected to the inner side of the inner connecting frame 401. An inlet pipe 404 is fixedly connected to one side of the liquid storage tank 402. A bottom water pump 405 is fixedly connected to the other side of the liquid storage tank 402. An outlet pipe 406 is provided on one side of the bottom water pump 405. A top water tank 407 is fixedly connected to one end of the outlet pipe 406. A top water pump 409 is fixedly connected to one side of the top water tank 407. A side spray pipe 4010 is provided on one side of the top water pump 409. An atomizing nozzle 4012 is fixedly connected to one end of the side spray pipe 4010. A top connecting hole 4011 is provided on the top of the incubator 1. A heat sink 408 is fixedly connected to one side of the top water tank 407.

[0034] It should be noted that the disinfectant in the top water tank 407 is drawn out along the side spray pipe 4010 by the top water pump 409. By opening the top connecting hole 4011, one end of the side spray pipe 4010 is fixed inside the incubator 1. At this time, the disinfectant flowing out of the side spray pipe 4010 is sprayed out in an atomized form by the atomizing nozzle 4012. The heat dissipation fin 408 on one side of the top water tank 407 increases the heat dissipation area, reduces the temperature of the disinfectant in the top water tank 407, and improves the stability of the disinfectant.

[0035] In this embodiment, an inner fixing plate 501 is fixedly connected to the inner side of the top water tank 407, a top bearing plate 502 is movably connected to one side of the inner fixing plate 501, a transmission plate 503 is movably connected to one side of the top bearing plate 502, a transmission rod 504 is fixedly connected to one side of the transmission plate 503, and an agitator 505 is fixedly connected to one end of the transmission rod 504.

[0036] It should be noted that the rotating transmission plate 503 will drive the transmission rod 504 to rotate, and the rotating transmission rod 504 will drive the agitator 505 to agitate, thereby agitating the disinfectant in the top water tank 407 and improving the activity of the disinfectant.

[0037] In this embodiment, the top of the incubator 1 is provided with a top protective cylinder 601, the inner side of the top protective cylinder 601 is fixedly connected with a top mounting hole 602, the top of the top mounting hole 602 is fixedly connected with a top fixing bracket 603, one side of the top fixing bracket 603 is fixedly connected with a motor body 604, the output shaft of the motor body 604 is fixedly connected with a connecting rod 605, and one end of the connecting rod 605 is fixedly connected with a transmission fan 606.

[0038] It should be noted that the rotating connecting rod 605 will drive the transmission fan 606 to rotate along the inner side of the top mounting hole 602, and extract the gas in the incubator 1, accelerate the evaporation of the disinfectant in the incubator 1, reduce residue, and improve the disinfection effect in the incubator 1.

[0039] In this embodiment, a metal side plate 609 is fixedly connected to the top of the top mounting hole 602. A side mounting hole 6011 is provided on one side of the metal side plate 609. A rubber ring 6010 is fixedly connected to the top of the metal side plate 609. A sealing plate 6012 is provided on one side of the metal side plate 609. A friction block 6013 is fixedly connected to the top of the sealing plate 6012. A magnetic suction plate 6014 is fixedly connected to one side of the sealing plate 6012. An arc-shaped groove 6015 is provided on one side of the sealing plate 6012 and one side of the metal side plate 609.

[0040] It should be noted that after the metal side plate 609 and the sealing plate 6012 are installed, the two arc-shaped grooves 6015 will fit with the connecting rod 605, and the rubber ring 6010 on the top of the metal side plate 609 will seal the arc-shaped grooves 6015, which will improve the sealing degree of the top mounting hole 602 after the sealing plate 6012 and the metal side plate 609 are installed. Furthermore, the magnetic attraction between the side mounting hole 6011 and the magnetic plate 6014 will improve the stability of the connection between the sealing plate 6012 and the metal side plate 609.

[0041] In this embodiment, a side bearing disk 607 is movably connected to one side of the transmission fan 606, and a bottom fixing frame 608 is movably connected to one side of the side bearing disk 607. The two sides of the bottom fixing frame 608 are fixedly connected to the inner side of the top mounting hole 602.

[0042] It should be noted that when the drive fan 606 rotates, it is movably supported on one side of the bottom fixed frame 608 via the side bearing plate 607, which improves the stability of the rotation of the drive fan 606.

[0043] The specific usage and function of this embodiment: The operator rotates the four gripping discs 304 according to the level of the placement surface. The rotation of the four gripping discs 304 drives the threaded column 305 to rotate. The bottom bearing disc 303 is used to movably connect the gripping discs 304 to the bottom of the incubator 1, maintaining the stability of the gripping discs 304 and the threaded column 305 during rotation. The threaded connection between the threaded column 305 and the threaded hole 307 causes the support block 306 to move vertically up and down along the inner side of the side lifting frame 302 when the threaded column 305 rotates. When the support block 306 moves vertically, it causes the sliding block 308 to slide along the inner side of the side slide rail 309, improving the stability of the support block 306 during vertical movement. By adjusting the height of the four support blocks 306 respectively, the tilt of the incubator 1 can be adjusted to maintain the levelness of the incubator 1 on different tilted placement surfaces, reducing the impact of tilt on cell activity.

[0044] Meanwhile, by fixing the storage tank 402 inside the inner connecting frame 401 and adding disinfectant through the inlet pipe 404, the disinfectant will flow into the storage tank 402 along the inlet pipe 404. When disinfecting the incubator 1 after the culture experiment is completed, the bottom water pump 405 is started to pump the disinfectant in the storage tank 402 into the top water tank 407 along the outlet pipe 406. The top water pump 409 is started to pump the disinfectant in the top water tank 407 out along the side spray pipe 4010. By opening the top connecting hole 4011, one end of the side spray pipe 4010 is fixed inside the incubator 1. At this time, the disinfectant flowing out of the side spray pipe 4010 is sprayed out in an atomized form through the atomizing nozzle 4012 to disinfect the incubator 1. This reduces the workload of the operators and eliminates the need to open the incubator 1, reducing the possibility of introducing external pollutants during manual disinfection.

[0045] Simultaneously, when the disinfectant enters the top water tank 407 along the outlet pipe 406, it impacts the transmission plate 503. The fan-shaped transmission plate 503 with an inclined angle rotates along the top bearing plate 502 after being impacted by the liquid. The transmission plate 503 is connected to the inner fixed plate 501 to maintain the free and stable rotation of the transmission plate 503 in the top water tank 407. The rotating transmission plate 503 drives the transmission rod 504 to rotate, and the rotating transmission rod 504 drives the agitator 505 to agitate the disinfectant in the top water tank 407, thereby increasing the activity of the disinfectant, reducing sedimentation, and improving the disinfection effect when the disinfectant is sprayed out along the atomizing nozzle 4012.

[0046] After disinfection, pressing the friction block 6013 pushes the sealing plate 6012. The magnetic plate 6014 is magnetically connected to the side mounting hole 6011. Applying external force causes the magnetic plate 6014 to separate along the inner side of the side mounting hole 6011. After separation, the sealing plate 6012 separates along one side of the metal side plate 609. After removing the sealing plate 6012 along the top of the top mounting hole 602, the motor body 604 is started. The motor body 604 drives the connecting rod 605 to rotate. The rotating connecting rod 605 drives the transmission fan 606 to rotate along the inner side of the top mounting hole 602, extracting the gas in the incubator 1, accelerating the evaporation of the disinfectant in the incubator 1, and reducing residue. To improve the disinfection effect inside the incubator 1, after the operation is completed, stop the rotation of the drive fan 606 and install the sealing plate 6012 and the metal side plate 609. Both the sealing plate 6012 and the metal side plate 609 have arc-shaped grooves 6015 on one side. After the metal side plate 609 and the sealing plate 6012 are installed, the two arc-shaped grooves 6015 will fit with the connecting rod 605, and the rubber ring 6010 on the top of the metal side plate 609 will seal the arc-shaped grooves 6015, which improves the sealing degree of the top mounting hole 602 after the sealing plate 6012 and the metal side plate 609 are installed. In addition, the magnetic attraction between the side mounting hole 6011 and the magnetic plate 6014 improves the stability of the connection between the sealing plate 6012 and the metal side plate 609.

[0047] The contents not described in detail in this specification are existing technologies known to those skilled in the art and are not key to this utility model, therefore they will not be elaborated upon.

[0048] The technical scope of this utility model is not limited to the content described above. Those skilled in the art can make various modifications and variations to the above embodiments without departing from the technical concept of this utility model, and all such modifications and variations should fall within the protection scope of this utility model.

Claims

1. An in vitro culture device for lung cancer tissue, comprising an incubator (1), characterized in that: An observation window (2) is fixedly connected to one side of the incubator (1). A bottom support frame (301) is fixedly connected to the bottom of the incubator (1). Side lifting frames (302) are provided on both sides of the bottom support frame (301). A bottom bearing plate (303) is movably connected to the bottom of the incubator (1). A grip plate (304) is movably connected to the bottom of the bottom bearing plate (303). A threaded column (305) is fixedly connected to the bottom of the grip plate (304). Support blocks (306) are provided on both sides of the bottom support frame (301). Threaded holes (307) are provided on the surface of the support blocks (306).

2. The in vitro culture device for lung cancer tissue according to claim 1, characterized in that: The side lifting frame (302) has side slide rails (309) on both sides, and a sliding block (308) is fixedly connected to one side of the support block (306). The surface of the sliding block (308) is slidably connected to one side of the side slide rail (309).

3. The in vitro culture device for lung cancer tissue according to claim 2, characterized in that: An inner connecting frame (401) is provided on the inner side of the bottom support frame (301), and a side connecting groove (403) is provided on the other side of the bottom support frame (301). A liquid storage tank (402) is fixedly connected to the inner side of the inner connecting frame (401). An inlet pipe (404) is fixedly connected to one side of the liquid storage tank (402), and a bottom water pump (405) is fixedly connected to the other side of the liquid storage tank (402). An outlet pipe (406) is provided on one side of the bottom water pump (405). One end of the liquid outlet pipe (406) is fixedly connected to a top water tank (407), a top water pump (409) is fixedly connected to one side of the top water tank (407), a side spray pipe (4010) is provided on one side of the top water pump (409), an atomizing nozzle (4012) is fixedly connected to one end of the side spray pipe (4010), a top connecting hole (4011) is opened on the top of the incubator (1), and a heat sink (408) is fixedly connected to one side of the top water tank (407).

4. The in vitro culture device for lung cancer tissue according to claim 3, characterized in that: An inner fixing plate (501) is fixedly connected to the inner side of the top water tank (407). A top bearing plate (502) is movably connected to one side of the inner fixing plate (501). A transmission plate (503) is movably connected to one side of the top bearing plate (502). A transmission rod (504) is fixedly connected to one side of the transmission plate (503). An agitator (505) is fixedly connected to one end of the transmission rod (504).

5. The in vitro culture device for lung cancer tissue according to claim 4, characterized in that: The incubator (1) has a top protective cylinder (601) on its top. The top protective cylinder (601) has a top mounting hole (602) fixedly connected to its inner side. The top mounting hole (602) has a top fixing frame (603) fixedly connected to its top. The top fixing frame (603) has a motor body (604) fixedly connected to one side. The output shaft of the motor body (604) has a connecting rod (605) fixedly connected to it. One end of the connecting rod (605) has a transmission fan (606) fixedly connected to it.

6. The in vitro culture device for lung cancer tissue according to claim 5, characterized in that: A metal side plate (609) is fixedly connected to the top of the top mounting hole (602). A side mounting hole (6011) is opened on one side of the metal side plate (609). A rubber ring (6010) is fixedly connected to the top of the metal side plate (609). A sealing plate (6012) is provided on one side of the metal side plate (609). A friction block (6013) is fixedly connected to the top of the sealing plate (6012). A magnetic suction plate (6014) is fixedly connected to one side of the sealing plate (6012). An arc-shaped groove (6015) is opened on one side of the sealing plate (6012) and one side of the metal side plate (609).

7. The in vitro culture device for lung cancer tissue according to claim 6, characterized in that: A side bearing plate (607) is movably connected to one side of the transmission fan (606), and a bottom fixing frame (608) is movably connected to one side of the side bearing plate (607). The two sides of the bottom fixing frame (608) are fixedly connected to the inside of the top mounting hole (602).

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